scholarly journals 391 Effects of increasing carbohydrases levels on productive performance of beef cattle fed feedlot finishing diets

2019 ◽  
Vol 97 (Supplement_3) ◽  
pp. 155-155
Author(s):  
Victor V Carvalho ◽  
Alexandre Perdigão ◽  
Tiago S Acedo ◽  
Guilherme S Vasconcellos ◽  
Luis Fernando Tamassia

Abstract Our objective was to evaluate the effects of feeding increasing exogenous carbohydrases levels on cattleˈ performance, when fed a 90-d feedlot finishing diet. A total of 240 Nellore bulls (IBW = 389 ± 27 kg) were distributed in 24 pens (10 animals/pen), in a randomized complete block design (6 pens/treatment). The basal diet was composed by 15% sugarcane bagasse, 72.8% ground corn, 7.6% soybean meal and 4.6 % mineral-vitamin premix (DM basis). The treatments were: 1) control: No addition of carbohydrases blend; 2) Blend1, in g/animal/d: 1 g of Ronozyme® WX (xylanase) + 3.75 g of Ronzyme® VP (beta-glucanase); 3) Blend2, in g/animal/d: 2 g of Ronozyme® WX + 7.5 g of Ronzyme® VP and 4) Blend3, in g/animal/d: 4 g of Ronozyme® WX + 15 g of Ronzyme® VP. The animals were fed once a day and had free access to fresh water. Statistical analysis was performed using the MIXED procedure of SAS 9.4, and comparisons were performed out by orthogonal contrasts, which compared the control and treatments, and the linear and quadratic effects of the increasing carbohydrases levels. Statistical significance was declared at P < 0.05, and tendencies considered when 0.05 < P < 0.10. Animals fed carbohydrases had greater (P = 0.045) ADG (1.59 vs. 1.51 kg/d) and tended to have greater (P = 0.069) final BW (533.4 vs. 527.7 kg) compared with control; although no difference were observed on these variables among carbohydrases levels. Feeding carbohydrases increased DM intake, expressed either as kg/d (10.17 vs. 9.92; P = 0.019) and as percentage of BW (2.20 vs. 2.16 %; P = 0.038), compared to control group, respectively. However, no difference was observed among treatments on G:F, and dressing percentage. In conclusion, the addition of carbohydrases blend can improve performance of finishing cattle.

2019 ◽  
Vol 97 (Supplement_3) ◽  
pp. 392-392
Author(s):  
Guilherme S Vasconcellos ◽  
Alexandre Perdigão ◽  
Tiago S Acedo ◽  
Victor V Carvalho ◽  
Luis Fernando Tamassia

Abstract The objective was to evaluate the effects of feeding levels of an exogenous carbohydrases blend on performance and carcass characteristics of grazing Nellore beef cattle. A total of 168 bulls were distributed to twenty Brachiaria brizantha cv. Xaraés paddocks (8 or 10 animals/paddock) in a randomized complete block design, with five replicates (paddocks) per treatment. Bulls were assigned according initial BW (IBW; 369.07±21.13 kg) to receive treatments for 231 days: 1) Control (basal mineral-protein-energetic supplement, no enzyme; PES), Carb1 (PES + 0.83 g of xylanase, RONOZYME® WX; and 3.09 g of beta-glucanase, RONOZYME® VP, both offered/kg PES), Carb2 (PES + 1.65 g of xylanase and 6.18 g of beta-glucanase/kg PES) and Carb3 (PES + 3.30 g of xylanase and 12.38 g of beta-glucanase/kg PES). Evaluated parameters were final BW (FBW), average daily gain (ADG), supplement intake, hot carcass weight (HCW) and dressing %. IBW and FBW were measured after a 12-hour fastening period. Treatments were offered daily in covered group feeders located in each paddock. Animals had free access to supplements and water. Supplements and enzymes were provided by DSM Nutritional Products. Data were analyzed using MIXED procedure of SAS® 9.3, being each paddock the experimental unit. Orthogonal contrasts were used to detect linear and quadratic effects on enzyme levels, with values P ≤ 0.05 considered significant. No linear or quadratic effects (P > 0.05) were observed on supplement intake (2.11 kg/animal/d), FBW (492.03 kg) and ADG (0.532 kg/day). However, a quadratic effect was observed for HCW and dressing %, with animals fed Carb1 presenting 5.69 kg heavier carcass (272.67 vs. 266.98; P = 0.039) and 1.4 p.p. higher dressing % (55.3% vs. 53.9%, P < 0.001) compared with control group. In conclusion, to use carbohydrases enzyme blend improves carcass characteristics and is an interesting technology for Nellore beef cattle grazed in tropical conditions.


2020 ◽  
Vol 98 (Supplement_4) ◽  
pp. 302-303
Author(s):  
Tainá E Martins ◽  
Tiago S Acedo ◽  
Vinicius N Gouvea ◽  
Guilherme S Vasconcellos ◽  
Mário B Arrigoni ◽  
...  

Abstract The objective was to evaluate the effects of 25-hydroxycholecalciferol (HyD®, DSM Produtos Nutricionais Brasil S.A.) supplementation in expression of genes related to anabolism and catabolism of feedlot cattle. A total of 120 Nellore bulls (IBW = 370±20 kg) were distributed in 24 fully roofed-pens with concrete floor (5 animals/pen) in a randomized complete block design (8 pens/treatment). The treatments were T1 (control): no supplementation of HyD®; T2: HyD® supplementation at 1 mg/animal/day; T3: HyD® supplementation at 3 mg/animal/day. Basal diet was formulated to meet requirements of finishing bulls, considering an ADG of 1.6 kg/day (Level 2 Nutrition System, Fox et al., 2004). Adaptation to diets followed a step-up scheme for 14 days, with concentrate inclusion being gradually increased from 76 to 91% DM. Finishing diets were offered from 15th to 100th day of feedlot period and animals fed twice a day (0800 and 1500), with bunks managed for a maximum of 5% orts. After slaughter, muscle samples were collected for quantitative evaluation of gene expression using RT-qPCR method, considering the following genes: SOD1 (antioxidant marker), IGF1, IGF2 and MTOR (anabolism), FOXO1, MURF1, Atrogin-1 and MSTN (catabolism). Statistical analysis was performed using PROC MIXED of SAS® and means compared by Tukey test at 5% probability. No significant differences among treatments were observed for SOD1, FOXO1, MURF1 and Atrogin-1 expression (P >0.05). However, tendencies (0.05 > P ≤0.10) could be observed for IGF1, IGF2, MTOR and MSTN expression in animals receiving HyD®, regardless of dosage. These results indicate a positive effect of HyD® on muscular anabolism and protein synthesis on feedlot finishing cattle. Moreover, the greater expression on MSTN suggest a higher protein turnover and muscular growth regulation. In conclusion, HyD® supplementation increased expression of genes correlated to muscular growth and protein synthesis, being a viable technology for beef cattle finished in feedlot systems.


2019 ◽  
Vol 97 (Supplement_2) ◽  
pp. 78-79
Author(s):  
Maryane S Faria de Oliveira ◽  
John K Htoo ◽  
Caroline J González-Vega ◽  
John E Thomson ◽  
Hans H Stein

Abstract An experiment was conducted to test the hypothesis that Val from a Val-containing fermentation biomass product (Val-FB; 64.4% L-Val) has a bioavailability of 100% relative to L-Val (98% Val) when fed to weanling pigs. A Val-deficient basal diet containing 0.63% standardized ileal digestible (SID) Val was formulated. Six additional diets were prepared by supplementing the basal diet with 0.08, 0.16, or 0.24% L-Val or 0.12, 0.25, or 0.37% Val-FB to create experimental diets containing 0.71, 0.79, or 0.87% SID Val. A total of 224 weanling pigs (6.87 ± 0.64 kg) were allotted to a randomized complete block design with 7 diets and 8 replicate pens per diet. Diets were fed for 21 d and growth performance was measured on a pen basis. Orthogonal-polynomial contrasts were used to determine linear and quadratic effects of L-Val and Val-FB levels on performance and the effect of Val sources. A linear regression model based on performance was used to estimate the relative bioavailability (RBV) of Val in Val-FB relative to L-Val. The final body-weight (BW) and average-daily-gain (ADG) were greater (P < 0.01) for pigs fed diets supplemented with Val-FB than pigs fed diets supplemented with L-Val (Table 1). The average-daily-feed-intake (ADFI) decreased linearly (P < 0.01), whereas gain-to-feed ratio (G:F) increased (P < 0.01) by supplementing graded levels of both Val sources to the diets. The RBV of Val in Val-FB as determined by ADG, G:F, and final BW was 146, 135, and 143%, respectively, with 95% confidence intervals of 99 to 191%, 83 to 187%, and 70 to 217%, respectively. In conclusion, the linear regression estimated a RBV of at least 100% for Val-FB relative to L-Val, and pigs fed diets supplemented with Val-FB had greater ADG and final BW than pigs fed diets supplemented with the same amount of L-Val.


Author(s):  
Osuloye Oluwaseum Olayemi ◽  
Olojo Oluwatobi Omotola ◽  
Philip Abutu

Allium sativum commonly referred to as garlic has been known over the years for its medicinal and culinary purposes. It has also been reported to have several toxic effects when used excessively. However, the purpose of this study was to determine the histological effects of Allium sativum (garlic) powder on the liver of white rabbits at different dosages. Twenty rabbits were randomly divided int.o five groups with free access to food and water for a period of four weeks. Four groups B, C, D, and E were fed with garlic supplemented basal diet containing different concentrations of garlic powder i.e. 100mg, 200mg, 500mg, and 1000mg respectively. These groups were called the treated group. Group A was fed with basal diet only and was considered as the control group. The results obtained showed some histological changes such as the presence of cellular necrosis, vacuolations, lipofuscin pigments, pyknosis and nuclear hypertrophy which were as a result of liver glycogen depletion and hepatic cell damage which may be due to relatively high dosage of garlic used in some of the groups.


2019 ◽  
Vol 97 (Supplement_2) ◽  
pp. 138-138
Author(s):  
Thomas M Winders ◽  
Melissa L Jolly-Breithaupt ◽  
Hannah C Wilson ◽  
James C MacDonald ◽  
Galen E Erickson ◽  
...  

Abstract Two trials were conducted to evaluate the effects of biochar (0, 0.8, or 3% of diet dry matter) on diet digestibility and methane production in growing and finishing cattle diets. The growing diet consisted of 21% brome hay, 20% wheat straw, 30% corn silage, 22% wet distillers grains plus solubles (WDGS), and 7% supplement. The finishing diet consisted of 53% dry rolled corn, 15% corn silage, 25% WDGS, and 7% supplement. Biochar replaced fine ground corn in the supplement. Growing diets were evaluated over 6 periods in a switchback design, followed by the finishing trial with 3 periods in a crossover design using 6 steers (529 kg initial BW). Digestibility measures were taken over 4 d after at least 8 d of adaptation to diets followed by 2 d of gas emission measurements using headbox calorimeters. Statistical analysis included treatment and period as fixed effects and steer as a random effect with PROC IML of SAS used to generate coefficients for orthogonal contrasts. In the growing study, OM and NDF digestibility increased quadratically (P = 0.10) while OM digestibility tended to linearly decrease (P = 0.13) and NDF digestibility was not affected (P ≥ 0.39) by biochar inclusion in the finishing diet. Methane production (g/d) tended to decrease quadratically (P = 0.14) in the growing study and was decreased 10.7% for the 0.8% biochar treatment relative to the control. There were no statistical differences in methane production (g/d) in the finishing study (P ≥ 0.32). Methane production (g/kg DMI) from the 0.8% biochar treatment relative to the control was numerically reduced 9.5% and 18.4% in the growing and finishing studies, respectively (P ≥ 0.13). Although biochar is not an FDA-approved feed for cattle, initial research shows potential as a methane mitigation strategy in both growing and finishing diets.


2014 ◽  
Vol 54 (8) ◽  
pp. 1016 ◽  
Author(s):  
R. Kachuee ◽  
M. Moeini ◽  
M. Souri

This study was conducted to determine the effects of organic and inorganic selenium (Se) supplementation during late pregnancy on serum Se status, colostrum, performance and passive immunity in Merghoz goats in Iran. Thirty Merghoz goats (30 ± 3 kg) were given the same basal diet containing 0.11 mg Se/kg DM and were randomly allocated to one of three dietary treatments in a randomised complete block design. Four weeks before the expected kidding, the basal diets were supplemented with 0 (control), 0.3 mg Se/head.day in the form of L-selenomethionin (Se-Met) and 0.3 mg Se/head.day in the form of sodium selenite. Blood samples were collected from the goats in three phases: (1) a couple of days before starting the supplementation; (2) before the expected kidding; and (3) on the day of kidding. Blood samples were also taken from the newborn kids from the jugular vein at birth and 7 days of age. The serum Se and immunoglobulin G (IgG) concentrations, the white blood cells (WBC) and differential leukocyte counts were measured. The reproductive parameters such as the number of kids born per kidding, total weight of kids born per goat mating, the pregnancy period and kid birthweights were determined. The results indicated that the serum Se concentration increased in supplemented goats compared with controls. The Se concentrations also significantly increased in the colostrum of treated goats (P < 0.05). Similarly, serum Se concentrations in kids of treated goats were increased at birth. On the other hand, colostrum and daily milk production were not affected by Se supplementation. Likewise, the change in the mean serum IgG levels was not different among goats and kids (P > 0.05). However, the WBC, neutrophil and lymphocyte counts were higher in the kids of goats in the Se-Met group compared with the control group on the day of birth and 7 days of age (P < 0.05). It seemed that Se-Met could have influenced the lamb’s immunity at birth and 7 days of age. By contrast, selenite has not affected the performance and passive immunity in Merghoz goats.


2020 ◽  
Author(s):  
Yu Zhang ◽  
Zhao-Xi Deng ◽  
Mao-Long He ◽  
Xin M. Luo ◽  
Jian-Xin Liu ◽  
...  

Abstract Background: The olive extract contains compounds with antioxidant and anti-inflammatory properties. This study was designed to investigate whether olive cake extract, enriched with maslinic acid and hydroxytyrosol, alleviates the lipopolysaccharides (LPS)-induced oxidative stress, inflammation and intestinal villus damage in piglets.Methods: Thirty weaned piglets (6.9±0.9 kg) were assigned to five groups using a randomized complete block design. Piglets were fed a basal diet before intraperitoneal (i.p.) injection of physiological saline (C); fed a basal diet alone (CL) or fed a basal diet plus olive extract (OL), antibiotics (AL), or olive extract and antibiotics (OAL) before i.p. injection of LPS. The feeding lasted for 2 weeks. Piglets were euthanized 4h after LPS injection. Systemic anti-oxidant and inflammation levels were measured and villus morphology in the intestine was examined.Results: Compared with those in the C group, piglets in the CL group had significantly lower GSH-Px, SOD, ALB levels and higher MDA, NO, LDH, ALT and AST levels in the serum (P<0.05). Compared with the CL group, piglets in OL, AL, and OAL groups had significantly higher serum GSH-Px, SOD and ALB levels and lower MDA, NO, LDH, ALT and AST levels (P<0.05). LPS administration significantly increased the serum concentration of TNF-α, IL-6, DAO and D-xylose in the CL group compared with the control group (P<0.05). Piglets in OL, AL, and OAL groups had significantly lower serum TNF-α, IL-6, DAO and D-xylose levels and higher IL-10 level (P<0.05). In the duodenum and ileum of piglets, LPS challenge led to significantly lower villus height (VH), higher crypt depth (CD) and lower VH/CD compared with the control group (P<0.05), whereas, OL, AL, and OAL groups had significantly lower CD and higher VH/CD compared with the CL group (P<0.05). Dietary inclusion of olive extract increased the relative abundance of intestinal Lactobacillus and Clostridium at genus level.Conclusion: Dietary supplementation with olive extract maslinic acid and hydroxytyrosol improved anti-oxidative and anti-inflammatory capacity, intestinal structure morphology, and increased the abundance of beneficial intestinal bacteria in weaned piglets challenged by LPS.


2021 ◽  
Vol 99 (Supplement_1) ◽  
pp. 59-60
Author(s):  
Adriana Morales Trejo ◽  
Verónica Sánchez ◽  
Bayron Pérez ◽  
Lucero R Camacho ◽  
J Caroline González-Vega ◽  
...  

Abstract The intestinal morphology can be compromised in pigs when exposed to heat stress (HS), partly due to increased production of reactive-oxygen species. Because methionine (Met) functions as intracellular antioxidant, requirement of Met may be increased in HS-pigs. The effect of dietary supplementation with DL-Met above requirement on performance and serum concentration (SC) of free AA in HS-pigs was evaluated. A basal wheat-soybean meal diet was formulated to meet 100% Met requirement with the other indispensable AA exceeding at least 20% their requirement. Sixty individually housed pigs (23.0 ± 2.4 kg BW, 12 pigs/treatment) were randomly assigned to 5 treatments: TN100, thermal-neutral (22.7 °C) housed pigs fed the basal diet; HS100, HS120, HS140, HS160; HS pigs (29.6 to 39.4°C) fed the basal diet supplemented with DL-Met to contain 0, 20, 40, and 60% DL-Met above the requirement, respectively. Pigs had free access to feed and water during the 21-d trial. Blood samples were collected on d18 to analyze the absorptive AA-SC. The effect of ambient temperature (HS100 vs. TN100), as well as the linear and quadratic effects of increasing Met levels in the diets for HS pigs were analyzed. The performance results for the TN100, HS100, HS120, HS140, HS160 pigs were: Average daily gain (ADG), 728, 612, 720, 716, 719 g/d; average daily feed intake, 1.40, 1.34, 1.30, 1.30, 1.29 kg/d; gain:feed, 0.522, 0.474, 0.569, 0.563, 0.562, respectively. The ADG reduced (P &lt; 0.01) in HS100 compared with TN100 pigs, but linearly increased in HS-pigs, besides gain:feed (P ≤ 0.05), in response to DL-Met supplementation. The SC of Ile, Leu, Lys, Phe, and Val were higher in HS100 pigs than in TN100 pigs (P &lt; 0.05). Graded supplemental DL-Met in diets for HS-pigs linearly decreased SC of Ile, Leu, and Val (P &lt; 0.05), tended to decrease His, Lys, and Thr (P &lt; 0.10), and increased Met (P &lt; 0.01). In conclusion, HS had negative effect on weight gain; however, it was ameliorated by adding 20% Met above the requirement.


2021 ◽  
Vol 99 (Supplement_1) ◽  
pp. 128-128
Author(s):  
John Gibbons ◽  
Andrea K Watson ◽  
Galen E Erickson ◽  
Tyler Spore ◽  
Hannah Wilson ◽  
...  

Abstract An experiment was conducted to evaluate the digestibility of a novel liquid feed, Condensed Algal Residue Solubles (Veramaris, Blair, NE) in finishing cattle diets. Mass production of algae to harvest omega-3 fatty acids results in byproduct production of CARS (25.4% DM, 19.3% CP, 8.3% Fat, 9.96% Na on DM basis), made up of the de-oiled algae cells and residual fermentation substrates. The CARS product has generally recognized as safe (GRAS) status. Six ruminally and duodenally cannulated crossbred steers were assigned randomly to 1 of 3 treatments over 3 collection periods, for a 3 x 3 replicated Latin Square design. Treatments differed by increasing inclusion of CARS (0, 2.5, and 5% of diet DM) fed with CARS replacing steam flaked corn (72, 69.5, and 67% of diet DM as CARS inclusion increased). All diets contained 15% dry distillers grains, 8% alfalfa haylage, and 5% supplement. Cattle were dosed with 10 g of titanium dioxide per day. Duodenal and fecal samples were collected four times per day across four days and composited by period for each animal. Fecal samples were analyzed for titanium dioxide concentration to determine fecal output and diet digestibility. Data were analyzed with CARS inclusion and period as fixed effects and animal as a random effect. Orthogonal contrasts were used to test linear and quadratic effects of CARS inclusion. There were no significant differences for DM intake and OM intake between the different CARS inclusions (P ≥ 0.17), averaging 7.76 kg DM/d and 6.94 kg OM/d. Total tract DM digestibility was not affected by treatment (P ≥ 0.71) and averaged 73.0%. Total tract OM digestibility was also not different between treatments (P ≥ 0.93) and averaged 71.1%. Replacing steam flaked corn with CARS up to 5% of diet DM in finishing cattle diets did not affect diet digestibility.


2019 ◽  
Vol 41 (3) ◽  
pp. 262-267
Author(s):  
Maria Angélica Rates Regalla ◽  
Daniel Segenreich ◽  
Priscilla Rodrigues Guilherme ◽  
Paulo Mattos

Abstract Objectives To investigate resilience levels in adolescents with attention-deficit hyperactivity disorder (ADHD) using quantitative measures when compared to their non-affected siblings and controls. We also aimed to investigate the correlation between resilience and depression, anxiety, intelligence quotient (IQ) and socioeconomic status, which may affect resilience levels and be potential confounders. Methods Adolescents (n=45) diagnosed with ADHD referred to an outpatient ADHD clinic, and their siblings without ADHD (n=27), with ages ranging from 12 to 17 years, were interviewed along with their parents using a semi-structured interview (Children’s Interview for Psychiatric Syndromes - Parent Version). Intelligence was measured with the Block Design and Vocabulary subtests from the Wechsler Battery. Anxiety and depression were investigated using the Children State-Trait Anxiety Inventory (CSTAI) and the Child Depression Inventory (CDI), respectively. Resilience was investigated using the Resilience Scale. A control group (typically developing adolescents [TDA] and their siblings; n=39) was recruited in another outpatient facility and at two schools using the same methodology. Results Socioeconomic status and intelligence levels, which may affect resilience, were similar in all groups. Adolescents with ADHD showed lower resilience levels compared to siblings and TDA even when controlled for anxiety and depression levels, which were higher in ADHD. Resilience levels were higher in siblings than in adolescents with ADHD, and lower than in TDA – this last result without statistical significance. Conclusion In our sample, ADHD in adolescents was associated with lower resilience, even when controlled for confounders often seen in association with the disorder.


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