PSX-B-29 Using ozonized flaxseed oil for treatment of postpartum endometritis

2021 ◽  
Vol 99 (Supplement_3) ◽  
pp. 272-272
Author(s):  
Semen Nikolaev ◽  
Igor Konopeltsev

Abstract Postpartum endometritis is one of the leading causes of infertility in cattle. The study aims to investigate the possibility of using ozonized flaxseed oil (OFO) for treating postpartum purulent-catarrhal endometritis in cows. Ozone was synthesized by using medical ozone generator and chemically pure oxygen. Linseed oil (400.0 ml) was bubbled with an ozone-oxygen mixture for 4 hours through a ceramic sprayer. An ozone concentration at the outlet was 30 mg/l. The therapeutic efficacy of OFO was studied on cattle of the Kholmogory breed with a productivity of 4800 - 5800 kg of milk per year. Thirty animals with symptoms of postpartum purulent-catarrhal endometritis were divided into two groups (n = 15). All of the subjects were in the second and third lactation periods. Animals from both groups were injected with 2% sinestrol solution on days 1 and 3 of treatment. OFO was used in the experimental group (EG). The cattle of the control group (CG) received a 7.5% solution of benzethonium chloride. Drugs were administered intrauterine at a dose of 50–150 ml (depending on the uterus’s size) using a polystyrene pipette, with an interval of 48–72 hours. Cows were inseminated artificially by the cervical method. The pregnancy was determined on the 30-35th day after insemination by ultrasonography. After 5 months of observation, all animals of the CG were pregnant. The pregnancy rate in the EG was 93,3%. In the groups efficiency of the first insemination was 20% and 47%, respectively. In the EG, one impregnation required 1.8±0.2 inseminations, which is 0.5 less than in the CG (2.3±0.3). The duration from calving to impregnation in cows in the EG was 104.4±6.9, 21.2 days less (P < 0.05) than the control, where the period was 125.6±7.6. Thus, OFO can be effectively used for the treatment of endometritis in cattle.

2020 ◽  
Vol 17 ◽  
pp. 00217
Author(s):  
Semen Nikolaev ◽  
Igor Konopeltsev

The search for alternative drugs for treating inflammatory diseases of bacterial etiology in high yielding cows that do not cause antibiotic resistance and do not reduce the quality of the obtained products is an urgent task for veterinary obstetrics. As one of this remedy, the authors have tested ozonated flaxseed oil (OFO), obtained by bubbling flaxseed oil with an ozon-oxygen mixture for four hours by means of ceramic spray with the ozone concentration being 30 mg / liter. During the bacteriological studies, it was found out that a two-hour incubation of the drug with a bacterial culture inhibits the growth of Staphylococcus aureus and Escherichia coli at a dilution of 108 microbial bodies (m.b.) in ml, bacteria of the genus Citrobacter at a concentration of 106 m.b. and Streptococcus agalactiae at the dilution of 107 m.b. in ml. The therapeutic efficiency of OFO was evaluated on the basis of using it for the cows of the Kholmogory Holstein breed, with the signs of postpartum septic catarrhal endometritis. For this purpose, 2 groups of animals were formed on the basis of analogues. For treating the cows of the experimental group the OFO has been used, and the cows of the control group were treated with the antibiotic containing the drug tylosinocar. For 5 months of observation, all animals were fertilized in the control group, and in the group where OFO was used this number was 93.3 %. Moreover, in the experimental group 47 % of the cows were fertilized after the first insemination, which is 27 % more in comparison with the control group. The duration from calving to fertilization in the group where OFO was used averaged 104 days, which is 21 days less (P <0.05) compared to the control group. Studies have shown that the use of OFO did not lead to the inhibitors emergence in milk in the experimental group of cows.


2012 ◽  
Vol 81 (2) ◽  
pp. 159-162 ◽  
Author(s):  
Petra Hudečková ◽  
Lucie Rusníková ◽  
Eva Straková ◽  
Pavel Suchý ◽  
Petr Marada ◽  
...  

The aim of this study was to compare the effect of two different types of oils in diet on the fatty acid profile in the eggs of layers and to include a particular type of oil as a supplement of feeding mixtures for layers in order to support the development of functional foodstuffs. Thirty layers fed a diet containing soybean oil constituted the control group (soybean oil is the most frequently used oil added to feeding mixtures). In the experimental group (thirty layers), soybean oil was replaced with linseed oil at the same amount (3 kg of oil per 100 kg of feeding mixture). Feeding was provided ad libitum for all days of the month. After one month, egg yolks were analysed and the fatty acid profile was compared. Significant differences (P ≤ 0.05) were found in the concentration of myristic acid that belongs to the group of saturated fatty acids. Eggs in the experimental group showed higher concentrations of myristic acid compared to the control group (0.20 g/100 g of fat and 0.18 g/100 g of fat, respectively). Highly significant differences (P ≤ 0.01) were found for heptadecanoic acid but the trend was opposite to that of myristic acid; concentrations of heptadecanoic acid in the experimental group were lower than those in the control group. Highly significant differences (P ≤ 0.01) were found for n-9 monounsaturated fatty acids where egg yolks in eggs from layers fed linseed oil contained higher concentrations of oleic acid, myristoleic acid, and palmitoleic acid. Lower concentrations of n-6 fatty acids (P ≤ 0.01) were found after the addition of linseed oil in eggs. Linseed oil showed a positive effect on n-3 fatty acids (α-linolenic acid), its concentration in the control and experimental group was 0.82 g/100 g of fat and 5.63 g/100 g of fat, respectively. The possibility of influencing the fatty acid profile in eggs is very important for the development of functional foods.


2021 ◽  
Vol 15 (4) ◽  
pp. 565-570
Author(s):  
Suhu Duan ◽  
Zaiqiang Li ◽  
Zhenzhen Fan ◽  
Mengran Qin ◽  
Xiaoxue Yu ◽  
...  

In order to study the effect of dietary supplement of linseed oil on polyunsaturated fatty acids (PUFAs) in egg yolk of Gallus domestiaus, total 160 healthy Gallus domestiaus of 26-week old were randomly selected and divided into 4 groups, each of which included 40 chickens fed outdoors with an area of 63 m2 and free food and drinking water. The control group (CK) was fed the basic diet, and the experimental group was added 1, 3 and 5% of linseed oil in the fundamentals of diet (experimental group 1, 2 and 3, abbreviated as P1, P2, P3), respectively. The content of PUFA and the value of n-6 PUFA and n-3 PUFA in egg yolk were determined by gas chromatography with 24 eggs randomly selected in each group after 10th, 25th and 40th days of the commence of the experiment. We found that the levels of α-linolenic acid (C18:3n3, ALA) and docosahexaenoic acid (C22:6n3, DHA) in egg yolk were clearly higher than the CK when 3% linseed oil or 5% linseed oil were added in the diet during the three experiment periods, in which the contents of linoleic Acid (18:2n6c, LA) and arachidonic acid (C20:4n6, ARA) in egg yolk differ little between P1, P2 and P3 and the CK while the value of n-6 PUFA and n-3 PUFA in egg yolk of the P2 and P3 groups decreased significantly. The comprehensive comparisons suggested that the content of C18:3n3 and C22:6n3 in egg yolk could be clearly increased in the P2 while the value of n-6 PUFA and n-3 PUFA decreased. This study provides theoretical basis for the production of functional eggs enriched with C22:6n3 and C18:3n3.


2021 ◽  
Vol 7 (5) ◽  
pp. 2624-2631
Author(s):  
Wei Di ◽  
Lei Wang ◽  
Qinghong Ji ◽  
Lihui Wang ◽  
Lifang An ◽  
...  

To study application effect of accompanying nursing mode and health education on infants with hypoxic ischemic encephalopathy (HIE) treated with hyperbaric oxygen therapy (HBOT) via pure oxygen chamber. 124 cases of HIE infants treated with pure oxygen chamberHBOT admitted to our department (December 2018-December 2019) were chosen as the research samples, and split into experimental group and control group according to the order of admission, with 62 cases in each group. The control group received routine nursing while and the experimental group received accompanying nursing mode combined with health education to compare the mental development index (MDI), psychomotor development index (PDI), height, weight, neurological sequelae, efficacy and family members’ satisfaction with HBOT between the two groups. After nursing, MDI and PDI in experimental group were obviously higher compared with control group (P<0.001);After nursing, the height and weight indexes in experimental group were obviously higher compared with control group (P<0.05);After nursing, the total incidence of neurological sequelae in experimental group (3.2%) was obviously lower compared with control group (P<0.05);After nursing, the total number of effective infants in experimental group was obviously higher compared with control group (P<0.05);After nursing, the number of family members satisfied with HBOT in experimental group was obviously higher compared with control group (P<0.05).The accompanying nursing mode combined with health education can greatly improve clinical indicators of HIE infants treated with pure oxygen chamber HBOT, promote their physical and mental development, and then improve the satisfaction of the family members with pure oxygen chamber HBOT, which should be promoted and applied in clinical practice as a high-quality nursing mode.


2019 ◽  
Author(s):  
O K Gogaev ◽  
A A Abaev ◽  
A R Demurova ◽  
E T Choniashvili

The aim of this research is to study the influence of ozone on the efficiency of incubating quail eggs. The study had been conducted in the period from 2014 to 2018 under the SIE LLC “EcoDom”. In the course of the experiment the Estonian quail egg were served as an object of research. To carry out the experiment a household ozonizer ”Groza” was used. The Eggs of the first “control” group were disinfected with formaldehyde vapors according to the standard method – 35 ml of 37 % formalin solution + 20 ml of tap water + 20 g of potassium permanganate per 1 cubic metre of volume in a special chamber. The other three groups were ozonized. The eggs of the 2nd experimental group were treated for 10 minutes exposure time, the third – 20 minutes and the fourth – 30 minutes, the ozone concentration in all experimental groups was the same and was 10 mg/m3. After treatment, the eggs were placed for brooding. Biochemical parameters of blood serum were determined by the analyzer ”Microlab-300”. Ozonation of hatching eggs contributes to increase crude protein in the quail’s blood serum by 9.37, 9.65 and 8.57 % compared to the control group. Hatching conditional quails was increased by 43.35 % in comparison with the control group.


Author(s):  
К.А. ГЕРЦЕВА ◽  
А.С. РОМАНОВА ◽  
Е.А. ШИШКИНА ◽  
М.В. ИЛЬИНА

Проблема и цель. Целью исследований стало изучение терапевтической эффективности комплексной терапии хронической обструктивной болезни бронхов и легких (ХОББиЛ) у лошадей и экономическое обоснование лечения. Методология. Научно-исследовательская экспериментальная работа проводилась на базе конюшни ИП Дубеев А.И. Малоярославецкого района Калужской области, ООО «Старожиловский конезавод», КСК «Успенский» Скопинского района, а также на базе частных конюшен Рязанской области в период с 2017-2019 гг. Объектом исследований являлись лошади с диагнозом ХОББиЛ. В процессе научной работы были собраны данные о распространении и манифестации ХОББиЛ на поголовье 16 лошадей различных пород, возраста и пола. На основании клинических и субклинических симптомов изучаемой патологии были составлены две схемы лечения ХОББиЛ у лошадей. В сравнительном аспекте изучена их терапевтическая эффективность, предоставлено экономическое обоснование терапии. Для этого были сформированы две аналогичные по диагнозу (ХОББиЛ) группы лошадей (n=8): контрольная и опытная. Результаты. Установлено, что заболеваемость лошадей ХОББиЛ за период с 2017 по 2019 годы составляет от 2,0 до 11,7 %. Установлена тенденция увеличения на 4,4 % случаев ХОББиЛ в 2019 году по сравнению с 2017 годом. Выявлено, что наивысший процент ХОББиЛ (53 %) наблюдался у животных старшей группы (16-19 лет). На протяжении периода наблюдения изучаемая патология в 75 % случаев была отмечена у жеребцов, в 25 % – у кобыл. Изучением манифестации проявления ХОББиЛ выявлено, что наиболее часто встречающимися симптомами во время приступов были: снижение работоспособности, снижение упитанности, абдоминальный тип дыхания, экспираторная одышка, увеличение частоты дыхания и сердцебиения в 100 %; западение межреберных промежутков, запальный желоб, выпячивание анального отверстия («игра анусом») – в 70 %, изменение пищевого поведения в 60 %, носовое истечение в виде густой катаральной пены в 50 % и лихорадка в 10 % случаев болезни у лошадей. Разработана эффективная комплексная схема терапии лошадей с ХОББиЛ. Заключение. Установлено, что при применении комплексного лечения ХОББиЛ у лошадей, включающего коррекцию условий содержания, внесение в рацион кормовых добавок «Equimins Clear Breather Supplement» и «Льняного масла», а также применение фармпрепаратов (дексаметазона, спутолизина, ингаляции беродуала) терапевтическая эффективность в опытной группе составила 87,5 %, что выше показателя контрольной группы на 12,5 %. Срок клинического выздоровления в опытной составил 9,8 суток, в контрольной группе 17,6 суток. Стоимость лечения ХОББиЛ в расчете на лошадь массой 500 кг составила 30530 руб., что в 2,2 раза больше чем в контроле. Однако это дорогостоящее лечение экономически обосновано, так как позволяет снизить сроки достижения ремиссии в 1,8 раза. Problem and goal. The aim of the research was to study the therapeutic effectiveness of complex therapy of chronic obstructive pulmonary disease (COPD) in horses with and provide an economic justification for treatment. Methodology. Research work was carried out on the basis of IP Dubeev A. I. Maloyaroslavetsky district of the Kaluga region, LLC "Starozhilovsky Stud Farm", KSK "Uspensky" of the Skopinsky district, as well as on the basis of private stables of the Ryazan region in the period from 2017-2019. The object of research was horses with a diagnosis of COPD. In the course of scientific work, data were collected on the manifestation of hobbils on the livestock of 16 horses of various breeds and ages. Based on the clinical and subclinical symptoms of the studied pathology, two treatment regimens for COPD in horses were compiled. In a comparative aspect, their therapeutic and economic effectiveness is studied. For this purpose, two groups of horses (n=8) similar in diagnosis (COPD) were formed: a control group and an experimental group. Results. It was found that the incidence of COPD horses for the period from 2017 to 2019 is from 2.0 to 11.7 %. The trend of an increase of 4.4% in cases of COPD in 2019 compared to 2017 was established. It was revealed that the highest percentage of hobbits (53 %) was observed in animals of the older group (16-19 years old). During the follow - up period, the studied pathology was observed in 75% of cases in stallions, and in 25% in mares. Studying the manifestation of the manifestation of COPD, it was revealed that the most common symptoms during seizures were: decreased performance, decreased fatness, abdominal type of breathing, expiratory shortness of breath, increased respiratory rate and heartbeat in 100 %, occlusion of the intercostal spaces, the ignition chute, protrusion of the anal opening ("playing with the anus") - in 70 %, changes in eating behavior in 60 %, nasal discharge in the form of thick catarrhal foam in 50% and fever in 10% of cases of the disease in patients with An effective comprehensive treatment regimen for horses with COPD has been developed. Conclusion. It was found that when using complex treatment of COPD in horses, including correction of conditions of detention, introduction of feed additives "Equimins Clear Breather Supplement" and "Linseed oil" into the diet, as well as the use of pharmaceuticals (dexamethasone, sputolysin, berodual inhalation), the therapeutic effectiveness in the experimental group was 87.5%, which is 12.5% higher than in the control group. The period of clinical recovery in the experimental group was 9.8 days, in the control group 17.6 days. The cost of treatment of COPD per horse weighing 500 kg was 30530 rubles, which is 2.2 times more than in the control. However, this expensive treatment is economically justified, as it reduces the time to achieve remission by 1.8 times.


2017 ◽  
Vol 73 (12) ◽  
pp. 792-796
Author(s):  
Žilvinas Vaškas ◽  
Artūras Šiukščius ◽  
Violeta Razmaitė ◽  
Alius Pockevičius ◽  
Nomeda Juodžiukynienė ◽  
...  

Many researchers have pointed out that the intake of omega-3 and -6 fatty acids is beneficial to animal health and reproductive capacity, but the effect of fatty acids on the histomorphology of ram testes is still unknown. The aim of this study was to investigate the effect of linseed oil containing polyunsaturated fatty acids on the morphology and histomorphometric characteristics of ram testis and epididymis. Testes of 12 Lithuanian local coarse wool rams were investigated histomorphologically and histomorphometrically in the present work. The rams were divided into two groups, an experimental group and a control group following, the principle of analogues – the same origin, age, weight, and condition score. The experimental group (n = 6) received 2% linseed oil sediments while the control group (n = 6) received 1.2% sunflower oil for 9 months. The diameter of testicular tubules and the height of the germinal epithelium, the spermatogenic index and the number of Leydig cells were higher in the experimental group than those in the control group. In addition, the number of degenerated tubules was not significantly lower in the group that received 2% linseed oil sediments. Still, the height of the epididymal epithelium was lower than that in the control group....


Author(s):  
D.C. Dominguez ◽  
J.T. Ellzey

Peroxisomes which participate in 1ipid metabolism have been shown to be altered in several metabolic disorders and toxic conditions. In alcoholic liver disease, the single lesion most frequently found is lipid accumu1ation in hepatocytes. However, the mechanisms for this 1ipid accumu1ation are not clear. The occurrence of modifications of liver peroxisomes due to excess alcohol consumption has not been subjected to a controlled study. We utilized a combination of cytochemica1 and morphometrictechniques to study the size and number of liver peroxisomes in rats fed an alcohol-supplemented diet compared to those of matched-paired control animals.Male Sprague-Daw1ey rats (400-500 g) received a liquid diet. The experimental group (N = 5/group) was fed a diet containing 30% ethanol-derived calories (EDC) and the control group was fed an isocaloric diet to 30% EDC. A pair feeding procedure was employed to control for caloric intake. Small pieces of liver randomly selected, were fixed in 2.3% -glutaraldehyde in 0.1 M sodium cacodylate buffer, pH 7.2, incubated in a DAB medium and postfixed with. 2% aqueous osmium tetroxide. EM photographs were taken from sections of 3 tissue blocks from each sample (7,200X) with a Zeiss EM10-A (60 kV). With the use of a point counting method and a digital planimeter the volume density (Vv) and numerical density (Nv) were determined.


1999 ◽  
Vol 58 (4) ◽  
pp. 263-272
Author(s):  
Jörg Doll ◽  
Michael Dick

The studies reported here focus on similarities and dissimilarities between the terminal value hierarchies ( Rokeach, 1973 ) ascribed to different groups ( Schwartz & Struch, 1990 ). In Study 1, n = 65 East Germans and n = 110 West Germans mutually assess the respective ingroup and outgroup. In this intra-German comparison the West Germans, with a mean intraindividual correlation of rho = 0.609, perceive a significantly greater East-West similarity between the group-related value hierarchies than the East Germans, with a mean rho = 0.400. Study 2 gives East German subjects either a Swiss (n = 58) or Polish (n = 59) frame of reference in the comparison between the categories German and East German. Whereas the Swiss frame of reference should arouse a need for uniqueness, the Polish frame of reference should arouse a need for similarity. In accordance with expectations, the Swiss frame of reference significantly reduces the correlative similarity between German and East German from a mean rho = 0.703 in a control group (n = 59) to a mean rho = 0.518 in the experimental group. Contrary to expectations, the Polish frame of reference does not lead to an increase in perceived similarity (mean rho = 0.712).


Author(s):  
J. Santoantonio ◽  
L. Yazigi ◽  
E. I. Sato

The purpose of this study was to investigate the personality characteristics in adolescents with SLE. The research design is a case-control study by means of the Rorschach Method and the Wechsler Intelligence Scale. Study group: 30 female adolescents with lupus, 12–17 years of age. The SLE Disease Activity Index was administered during the period of psychological evaluation. Control group: 32 nonpatient adolescents were matched for age, sex, and socioeconomic level. In the Wechsler Intelligence Scale the mean IQ of the experimental group was significantly lower than that of the control group (77 and 98, respectively, p < .001). In the Rorschach, the lupus patients showed greater difficulty in interpersonal interactions, although they displayed the resources to process affect and to cope with stressful situations. A positive moderate correlation (p = .069) between the activity index of the disease and the affect constriction proportion of the Rorschach was observed: the higher the SLEDAI score, the lower the capacity to process affect. There is a negative correlation between the activity index of the disease and the IQ (p = .001): with a higher activity index of the disease, less intellectual resources are available.


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