PSXIII-18 The effect of lead on alkaline phosphatase serum activity in European carp

2021 ◽  
Vol 99 (Supplement_3) ◽  
pp. 434-435
Author(s):  
Polina D Bokhan ◽  
Polina A Polistovskaia ◽  
Larisa Y Karpenko ◽  
Abram I Enukashvili ◽  
Alesya A Bakhta ◽  
...  
Keyword(s):  
Heavy Metals ◽  
Russian Federation ◽  
Lead Acetate ◽  
Control Group ◽  
Acetate Concentration ◽  
Permissible Exposure Limit ◽  
Serum Activity ◽  
Exposure Limit ◽  
The Russian Federation ◽  
Experimental Group

Abstract The aim of this research is to assess the toxic influence of heavy metals in fish. This study was intended to analyze alkaline phosphatase (AP) serum activity under the influence of various lead concentrations in European carp. AP is involved in the dephosphorylation processes and is an important indicator of liver and skeleton tissue metabolism. When exposed to heavy metals, a change in the activity of this enzyme can be observed. In this experiment 3 groups of fish (European carp) were formed, 10 fish in each group: control group; 1-experimental group – fish were contained in water with 0.06 mg/l lead acetate concentration (upper threshold level than fishery waters permissible exposure limit according to the laws of the Russian Federation); 2-experimental group – fish were contained in water with 0.6 mg/l lead acetate concentration (10 times higher than fishery waters permissible exposure limit according to the laws of the Russian Federation). Exposure time was 4 hours. The blood samplings were taken from heart by syringe and placed in dry test tubes. After samplings centrifugation in non-hemolysed serum samples AP activity was assessed. The study revealed the decreasing trend of AP serum activity by 1.08% (21.89±0.24 IU/l) in 1-experimental group in compare with control group (22.13±0.78 IU/l). In case where the lead acetate concentration were 10 times higher than fishery waters permissible exposure limit were meaningful decreasing of AP activity (P ≤ 0.05) by 66.23% (17.42±0.63 IU/l) accordingly in 2-experimental group in compare with control group. In conclusion, a statistically significant decrease in the AP activity in fish blood serum was found after acetate concentration 10 times higher than the permissible exposure limit in fishery waters, according to the laws of the Russian Federation. We can suppose that the activity of this enzyme is the reason for the inhibitory effect of lead on liver tissue.

scholarly journals PSXI-25 The effect of lead on aminotransferases serum activity in European carp

2019 ◽  
Vol 97 (Supplement_3) ◽  
pp. 381-382
Author(s):  
Polina A Polistovskaia ◽  
Abram I Enukashvili ◽  
Anna I Kozitcyna ◽  
Katerina P Kinarevskaia ◽  
Alesya A Bakhta ◽  
...  
Keyword(s):  
Russian Federation ◽  
Lead Acetate ◽  
Activity Level ◽  
Control Group ◽  
Acetate Concentration ◽  
Permissible Exposure Limit ◽  
Serum Activity ◽  
Exposure Limit ◽  
The Russian Federation ◽  
Experimental Group

Abstract The aim of this study is to asset toxic influence of heavy metals in fish. This research was intended to analyze aminotransferases (ALT, AST) serum activity under the influence of various lead concentrations in European carp. Intracellular enzymes activity level elevation in serum is a sign of mass cell destruction and consequently pathological process. In this experiment 3 groups of fish (European carp) were formed, 10 fish in each group: control group; 1-experimental group – fish were contained in water with 0.06 mg/l lead acetate concentration (10 times higher than fishery waters permissible exposure limit according to the laws of the Russian Federation); 2-experimental group – fish were contained in water with 0.6 mg/l lead acetate concentration (100 times higher than fishery waters permissible exposure limit according to the laws of the Russian Federation). Exposure time was 4 hours. The blood samplings were taken from heart by syringe and placed in dry test tubes. After centrifugation of the samplings in non-hemolysed serum samples ALT and AST activities were assessed by standard methods. The study revealed that there were meaningful elevation of ALT and AST serum activity (P ≤ 0.001) by 43.8% (30.83±1.14 IU/l) and 17.58% (266.52±6.37 IU/l) accordingly in 1-experimental group in compare with control group (21.44±2.17 IU/l and 226.68±4.35 IU/l). In case where the lead acetate concentration were 100 times higher than fishery waters permissible exposure limit there were meaningful elevation of ALT and AST serum activity (P ≤ 0.001) by 74.86 % (37.49±2.76 IU/l) and 66.23% (376.82±19.45 IU/l) accordingly in 2-experimental group in compare with control group. After analyzing the received data there were established meaningful aminotransferases serum activity elevation under the influence of various lead concentrations in European carp. This is possibly connected to lipid peroxygenation processes disruption in muscles and liver under the lead toxic influence.

scholarly journals PSXI-12 Effect of copper on aminotransferases serum activity in European carp

2020 ◽  
Vol 98 (Supplement_4) ◽  
pp. 390-390
Author(s):  
Artem O Taraskin ◽  
Polina A Polistovskaia ◽  
Abram I Enukashvili ◽  
Anna I Kozitcyna ◽  
Alesya A Bakhta ◽  
...  
Keyword(s):  
Russian Federation ◽  
Copper Acetate ◽  
Activity Level ◽  
Control Group ◽  
Acetate Concentration ◽  
Permissible Exposure Limit ◽  
Serum Activity ◽  
Exposure Limit ◽  
The Russian Federation ◽  
Experimental Group

Abstract The aim of this study is to assess toxic influence of heavy metals in fish. This research was intended to analyze aminotransferases (ALT, AST) serum activity under the influence of various copper concentrations in European carp. Intracellular enzymes activity level elevation in serum is a sign of hepatocites toxic destruction and an indicator of profound cell alterations. In this experiment 3 groups of fish (European carp) were formed, 10 fish in each group: control group; 1-experimental group – fish were contained in water with 0.01 mg/l copper acetate concentration (10 times higher than fishery waters permissible exposure limit according to the laws of the Russian Federation); 2-experimental group – fish were contained in water with 0.1 mg/l copper acetate concentration (100 times higher than fishery waters permissible exposure limit according to the laws of the Russian Federation). Exposure time was 4 hours. The blood samplings were taken from heart by syringe and placed in dry test tubes. After samplings centrifugation in non-hemolysed serum samples ALT and AST activities were assessed. The study revealed that there was meaningful elevation of ALT and AST serum activity (P ≤ 0.01) by 19.77% (25.68 ± 0,59 IU/l) and 16.84% (264.85 ± 7.6 IU/l) accordingly in 1-experimental group in comparison with control group (21.44 ± 0.72 IU/l и 226.68 ± 4.35 IU/l). In cases where the copper acetate concentration was 100 times higher than fishery waters permissible exposure limit there was meaningful elevation of ALT and AST serum activity (P ≤ 0.01) by 81,62 % (38.94 ± 0.83 IU/l) and 71.81% (389.46 ± 18.7 IU/l) accordingly in 2-experimental group in comparison with control group. After analyzing the received data, there were established meaningful aminotransferases serum activity elevation under the influence of various copper concentrations in European carp. This was possibly due to gluconeogenesis activation in order to support an adequate glucose level in conditions of catabolic process dominance during copper poisoning.

scholarly journals Evaluation of the effectiveness methods of initial pilots’ proffessional training to operate the aircraft equipped with electronic flight instrument systems

2018 ◽  
Vol 21 (5) ◽  
pp. 8-22
Author(s):  
S. G. Kosachevskiy ◽  
D. V. Aidarkin ◽  
D. V. Kachan
Keyword(s):  
Russian Federation ◽  
Professional Training ◽  
Flight Training ◽  
Simulator Training ◽  
Civil Aviation ◽  
Control Group ◽  
Series Of Experiments ◽  
The Russian Federation ◽  
New Generation ◽  
Experimental Group

Nowadays, educational institutions carrying out pilots’ training in the Russian Federation use completely new generation aircraft different with high degree of control automatisation (EFIS – Electronic Flight Instrument System). At the same time, the applied methods of flight training are based on studies carried out for aircraft with analog instruments, which does not allow to use new generation aircraft and simulators wide possibilities at  their full capacity. Therefore, there is a vital necessity of enhancing the method of pilots’ initial professional training that should contain teaching rational methods of distribution and switching visual attention. In 2017 in accordance with the Order of the Federal Air Transport Agency of the Russian Federation in the Ulyanovsk Institute of Civil Aviation a complex of research was carried out that allowed developing the method of initial pilots’ flight training on the aircraft equipped with EFIS. During the research, the oculometric research methods of cadets’ distribution and switching attention (the "eye-tracking" technology) were used, which allowed a deeper study of the piloting skills formation and their impact on pilot's operation with EFIS. To assess the effectiveness of the developed methods, two series of experiments involving cadets of Ulyanovsk Institute of Civil Aviation and its branch in Sasovo were conducted. Two types of simulators: L-410 and Diamond 40 NG were used for research purposes. In this article it is proposed to use the correlation coefficient and the Manhattan distance to assess the accuracy of maintaining flight parameters during cadet simulator training. According to the results of the first series of experiments, it was found out that the cadets under the experiment showed a lower level of flight training compared to the control group of cadets. However, after training the group under the experiment on the developed method, a level of flight preparation in the both groups became equal. Statistically significant differences of initial and final levels of flight training for the cadets of the experimental group were observed while cadets of a control group completed the series of experiments without any significant changes. The second series of experiments revealed that the lack of flight practice among the cadets of the experimental group did not prevent them from demonstrating a qualitative level of professional training when practicing on the flight simulator. Such a result was achieved due to the ground preparation of cadets on the developed method what, undoubtedly, indicates its effectiveness.

PROBLEMS OF AGROECOLOGICAL ASSESSMENT OF LAND PLOTS FOR THEIR SUITABILITY FOR ORGANIC PRODUCTION AND DETOXIFICATION OF SOILS CONTAMINATED WITH PESTICIDES AND HEAVY METALS

2020 ◽  
Author(s):  
N.N. Novikov ◽  
◽  
S.V. Mitrofanov ◽  
N.N. Grachev ◽  
M.M. Varfolomeeva ◽  
...  
Keyword(s):  
Heavy Metals ◽  
Russian Federation ◽  
Digital Technology ◽  
Environmental Safety ◽  
Organic Production ◽  
Federal Law ◽  
Physical Chemical ◽  
Organic Products ◽  
Contamination Of Soils ◽  
The Russian Federation

Based on the analysis of statistical yearbooks "state of contamination of soils and objects of the natural environment of the Russian Federation with pesticides and toxicants of industrial origin", analysis of the incidence of workers and the population from exposure to residual pesticides and heavy metals, the need to assess soil contamination for organic farming purposes in accordance with the requirements of article 9 of the Federal law of 03.08.2018 No. 280-FZ "on organic products and amendments to certain legislative acts of the Russian Federation"is justified. For evaluation, a digital technology is proposed in the management of environmental safety and labor protection in agriculture, developed by scientists of the ITOSH-branch of the FGBNU FNAC VIM. It is proposed to solve the problems of detoxification of soils contaminated with heavy metals and residual pesticides identified during the assessment using the available domestic and foreign experience by various methods: physical, physical-chemical, and biological.

scholarly journals CLINICAL AND IMMUNOLOGICAL EFFECTIVENESS OF TREATMENT OF RAGWEED POLLINOSIS IN THE SAMARA REGION

2021 ◽  
Vol 23 (4) ◽  
pp. 921-926
Author(s):  
N. N. Zhukova ◽  
M. V. Manzhos ◽  
L. R. Khabibulina ◽  
E. Yu. Syrtsova
Keyword(s):  
Allergic Rhinitis ◽  
Russian Federation ◽  
Allergic Diseases ◽  
Ambrosia Artemisiifolia ◽  
Control Group ◽  
Ambrosia Trifida ◽  
Results Of Treatment ◽  
Amb A 1 ◽  
The Russian Federation ◽  
Time Changes

Allergic rhinitis and bronchial asthma are widespread respiratory allergic diseases. In some territories of the Russian Federation, the dominant cause of pollinosis is ragweed. The aim of the study was to evaluate the clinical and immunological efficacy of ASIT with the allergen Ambrosia artemisiifolia in patients sensitized to Ambrosia trifida in the Samara region. Patients with proven sensitization to Ambrosia trifida was held immunotherapy with Ambrosia artemisiifolia allergoid preseason. After treatment, patients had a decrease in the severity of symptoms of allergic rhinitis according to VAS (p = 0.00001), a decrease in the need for medications (p = 0.0003), as well as the need for corticosteroids against the background of therapy from 34.6% to 0% (p = 0.00001). In 8% of cases, the result of treatment was good, in 69% satisfactory, in 23% unsatisfactory. In the control group, there were no changes in the severity of symptoms (p = 0.858). Also, in the control group, the need for medications remained unchanged and 14.3% of patients continued to use corticosteroids.After ASIT, there was a decrease in the level of IL-4 (p = 0.002), and a decrease in the ratio of IL-4/ IL-10 (p = 0.0063); at the same time, changes in the level of other cytokines (IL-10; IFNγ) were statistically insignificant (p > 0.05). Before treatment, the levels of IL-4/ IL-10 in both groups were comparable, and after treatment, the differences became statistically significant (p = 0.031). We did not get a statistically significant change in the level of IgG4 Amb a 1 or IgG4 Amb trifida. There was no correlation between the level of individual cytokines and the results of treatment. As a result of the conducted ASIT, positive clinical and immunological results were obtained. In most patients, the disease has acquired a controlled course. At the same time, the lack of excellent and low number of good results of ASIT is probably due to the intraspecific allergenic properties of ragweed. 

scholarly journals Biotechnological aspects of transformation of heavy metal salts and their detoxication in milk of milk cows

2021 ◽  
Vol 262 ◽  
pp. 02010
Author(s):  
Boris Dzagurov ◽  
Oleg Getokov ◽  
Vladimir Gukezhev ◽  
Taladin Kokov ◽  
Vitaly Vorokov
Keyword(s):  
Heavy Metals ◽  
Dairy Cows ◽  
Particle Diameter ◽  
Bentonite Clay ◽  
The Body ◽  
Control Group ◽  
Milk Cows ◽  
Heavy Metal Salts ◽  
Cadmium Lead ◽  
Experimental Group

Based on the results of previous studies on pigs and poultry, in which encouraging results of a significant decrease in the concentration of heavy metals in the body were obtained, respectively, in pork, poultry and eggs, the study provided for the use of the bentonite clay of the Zamankul deposit (RNO-Alania) as an enterosorbent in relation to heavy metals and detoxification of the organism of dairy cows. In order to study the feasibility of using bentonite as an enterosorbent, studies were performed on dairy cows of the Simmental breed in the Kaloyev farm located in st. Zmeyskaya, Kirovsky district of North Ossetia-Alania. To conduct research on the principle of analog pairs, two experimental groups of cows (control and experimental, five cows in each group) of the 3rd and 4th lactation were formed. The experiment lasted for 305 days of lactation from March 2018 to January 2019. Herewith, the control group of cows was fed with a basic diet balanced in all nutritional elements, the experimental livestock, together with the main diet, daily in the composition of the concentrates was injected with crushed bentonite with a particle diameter of 4-6 mm based on the dry matter of the feed (137 g/animal). The study of the concentration of heavy metals (cadmium, lead and zinc) in soil, feed, blood and milk was performed in two periods of lactation (on the 250th and 300th days of lactation). Herewith, an increased content of heavy metals in the soil was established, relative to the MPL: cadmium - 10.1; lead - 7.4; zinc - 9.7 times more, in drinking water: cadmium - 2.5 times; lead - 9.0 times and zinc - 9.7 times more, in feed: cadmium - from 1.7 to 5.3 times; lead - from 1.1 to 1.7; zinc - from 1.0 to 2.5 times the MPL. The inclusion of bentonite to the ration of the animals of the experimental group, in comparison with the control group, in both study periods (250 and 300 days of lactation) contributed to a significant decrease in the concentration of the studied heavy metals in the blood from 17 to 20%, in milk - from 16 to 18 %. The transformation ration values of heavy metals from feed into the body, respectively, into milk, were also lower in the animals of the experimental group, relative to the control.

scholarly journals PSXIV-5 Results of external use of staphylococcal toxoid for the prevention of mastitis in cows

2019 ◽  
Vol 97 (Supplement_3) ◽  
pp. 315-315
Author(s):  
Dariya Kashtanova ◽  
Valerii G Skopichev ◽  
Flura Alistratova ◽  
Polina A Polistovskaia ◽  
Nataliya A Panova ◽  
...  
Keyword(s):  
Russian Federation ◽  
Economic Losses ◽  
Economic Damage ◽  
Control Group ◽  
Dry Period ◽  
North West ◽  
Lactating Cows ◽  
The North ◽  
The Russian Federation ◽  
The Cost

Abstract Mastitis in cows in the farms of the Russian Federation cause great economic damage. Economic losses include the cost of treating cows and the cost of recycled milk. In the farms of the North-West region of the Russian Federation mastitis occurs in 20–30% of lactating cows. An important task is to find effective ways to prevent mastitis. The task of our research included the study of the effectiveness of external use of staphylococcal toxoid. For the experiment, 2 experimental groups of dry cows of 10 animals each were formed. For the first group of cows, 5 days before the expected date of calving, an ointment containing staphylococcal toxoid and dimethyl sulfoxide was applied to the area of the milk mirror. Udder treatment was performed once a day for five days. Cows of the second group were immunized with Starvac® vaccine in accordance with the instructions, twice during the dry period. The third group of cows (n = 20) was the control. After calving, the concentration of immunoglobulins in colostrum was examined in all cows. In cows of the first group, the concentration of immunoglobulins G, M and A in colostrum was 8.59 ± 0.9; 1.1 ± 0.13 and 5.42 ± 0.9 g / l, respectively. The concentration of immunoglobulins in cows of the first group was higher, on average, by 1.3–2.0 times in comparison with cows that were given the Starvac vaccine. In comparison with the control group, the growth of all classes of immunoglobulins was determined by 2.2 - 5.2 times (P < 0.01). The results obtained showed high efficacy of external use of staphylococcal toxoid together with dimethisulfoxide.

Hydrocarbon- and metal-polluted soil bioremediation: progress and challenges

2018 ◽  
Vol 39 (3) ◽  
pp. 133 ◽  
Author(s):  
Maria Kuyukina ◽  
Anastasiya Krivoruchko ◽  
Irina Ivshina
Keyword(s):  
Heavy Metals ◽  
Russian Federation ◽  
Petroleum Hydrocarbons ◽  
Recent Progress ◽  
Polluted Soil ◽  
Soil Bioremediation ◽  
Microbial Enzymes ◽  
Metal Pollutants ◽  
General Metabolism ◽  
The Russian Federation

The problem of soil contamination with petroleum hydrocarbons and heavy metals is becoming particularly acute for large oil-producing countries, like the Russian Federation. Both hydrocarbon and metal contaminants impact negatively the soil biota and human health, thus requiring efficient methods for their detoxification and elimination. Bioremediation of soil co-contaminated with hydrocarbon and metal pollutants is complicated by the fact that, although the two components must be treated differently, they mutually affect the overall removal efficiency. Heavy metals are reported to inhibit biodegradation of hydrocarbons by interfering with microbial enzymes directly involved in biodegradation or through the interaction with enzymes involved in general metabolism. Here we discuss recent progress and challenges in bioremediation of soils co-contaminated with hydrocarbons and heavy metals, focusing on selecting metal-resistant biodegrading strains and biosurfactant amendments.

THE RESULTS OF MONITORING OF HEAVY METALS IN FEED AND WATER IN SOME REGIONS OF THE RUSSIAN FEDERATION

2019 ◽  
Vol 240 (4) ◽  
pp. 109-114 ◽  
Author(s):  
V.A. Konychova ◽  
◽  
I.R. Kadikov ◽  
A.A. Korchemkin ◽  
K.H. Papunidi ◽  
...  
Keyword(s):  
Heavy Metals ◽  
Russian Federation ◽  
The Russian Federation
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