scholarly journals PSXI-12 Effect of copper on aminotransferases serum activity in European carp

2020 ◽  
Vol 98 (Supplement_4) ◽  
pp. 390-390
Author(s):  
Artem O Taraskin ◽  
Polina A Polistovskaia ◽  
Abram I Enukashvili ◽  
Anna I Kozitcyna ◽  
Alesya A Bakhta ◽  
...  
Keyword(s):  
Russian Federation ◽  
Copper Acetate ◽  
Activity Level ◽  
Control Group ◽  
Acetate Concentration ◽  
Permissible Exposure Limit ◽  
Serum Activity ◽  
Exposure Limit ◽  
The Russian Federation ◽  
Experimental Group

Abstract The aim of this study is to assess toxic influence of heavy metals in fish. This research was intended to analyze aminotransferases (ALT, AST) serum activity under the influence of various copper concentrations in European carp. Intracellular enzymes activity level elevation in serum is a sign of hepatocites toxic destruction and an indicator of profound cell alterations. In this experiment 3 groups of fish (European carp) were formed, 10 fish in each group: control group; 1-experimental group – fish were contained in water with 0.01 mg/l copper acetate concentration (10 times higher than fishery waters permissible exposure limit according to the laws of the Russian Federation); 2-experimental group – fish were contained in water with 0.1 mg/l copper acetate concentration (100 times higher than fishery waters permissible exposure limit according to the laws of the Russian Federation). Exposure time was 4 hours. The blood samplings were taken from heart by syringe and placed in dry test tubes. After samplings centrifugation in non-hemolysed serum samples ALT and AST activities were assessed. The study revealed that there was meaningful elevation of ALT and AST serum activity (P ≤ 0.01) by 19.77% (25.68 ± 0,59 IU/l) and 16.84% (264.85 ± 7.6 IU/l) accordingly in 1-experimental group in comparison with control group (21.44 ± 0.72 IU/l и 226.68 ± 4.35 IU/l). In cases where the copper acetate concentration was 100 times higher than fishery waters permissible exposure limit there was meaningful elevation of ALT and AST serum activity (P ≤ 0.01) by 81,62 % (38.94 ± 0.83 IU/l) and 71.81% (389.46 ± 18.7 IU/l) accordingly in 2-experimental group in comparison with control group. After analyzing the received data, there were established meaningful aminotransferases serum activity elevation under the influence of various copper concentrations in European carp. This was possibly due to gluconeogenesis activation in order to support an adequate glucose level in conditions of catabolic process dominance during copper poisoning.

scholarly journals PSXI-25 The effect of lead on aminotransferases serum activity in European carp

2019 ◽  
Vol 97 (Supplement_3) ◽  
pp. 381-382
Author(s):  
Polina A Polistovskaia ◽  
Abram I Enukashvili ◽  
Anna I Kozitcyna ◽  
Katerina P Kinarevskaia ◽  
Alesya A Bakhta ◽  
...  
Keyword(s):  
Russian Federation ◽  
Lead Acetate ◽  
Activity Level ◽  
Control Group ◽  
Acetate Concentration ◽  
Permissible Exposure Limit ◽  
Serum Activity ◽  
Exposure Limit ◽  
The Russian Federation ◽  
Experimental Group

Abstract The aim of this study is to asset toxic influence of heavy metals in fish. This research was intended to analyze aminotransferases (ALT, AST) serum activity under the influence of various lead concentrations in European carp. Intracellular enzymes activity level elevation in serum is a sign of mass cell destruction and consequently pathological process. In this experiment 3 groups of fish (European carp) were formed, 10 fish in each group: control group; 1-experimental group – fish were contained in water with 0.06 mg/l lead acetate concentration (10 times higher than fishery waters permissible exposure limit according to the laws of the Russian Federation); 2-experimental group – fish were contained in water with 0.6 mg/l lead acetate concentration (100 times higher than fishery waters permissible exposure limit according to the laws of the Russian Federation). Exposure time was 4 hours. The blood samplings were taken from heart by syringe and placed in dry test tubes. After centrifugation of the samplings in non-hemolysed serum samples ALT and AST activities were assessed by standard methods. The study revealed that there were meaningful elevation of ALT and AST serum activity (P ≤ 0.001) by 43.8% (30.83±1.14 IU/l) and 17.58% (266.52±6.37 IU/l) accordingly in 1-experimental group in compare with control group (21.44±2.17 IU/l and 226.68±4.35 IU/l). In case where the lead acetate concentration were 100 times higher than fishery waters permissible exposure limit there were meaningful elevation of ALT and AST serum activity (P ≤ 0.001) by 74.86 % (37.49±2.76 IU/l) and 66.23% (376.82±19.45 IU/l) accordingly in 2-experimental group in compare with control group. After analyzing the received data there were established meaningful aminotransferases serum activity elevation under the influence of various lead concentrations in European carp. This is possibly connected to lipid peroxygenation processes disruption in muscles and liver under the lead toxic influence.

PSXIII-18 The effect of lead on alkaline phosphatase serum activity in European carp

2021 ◽  
Vol 99 (Supplement_3) ◽  
pp. 434-435
Author(s):  
Polina D Bokhan ◽  
Polina A Polistovskaia ◽  
Larisa Y Karpenko ◽  
Abram I Enukashvili ◽  
Alesya A Bakhta ◽  
...  
Keyword(s):  
Heavy Metals ◽  
Russian Federation ◽  
Lead Acetate ◽  
Control Group ◽  
Acetate Concentration ◽  
Permissible Exposure Limit ◽  
Serum Activity ◽  
Exposure Limit ◽  
The Russian Federation ◽  
Experimental Group

Abstract The aim of this research is to assess the toxic influence of heavy metals in fish. This study was intended to analyze alkaline phosphatase (AP) serum activity under the influence of various lead concentrations in European carp. AP is involved in the dephosphorylation processes and is an important indicator of liver and skeleton tissue metabolism. When exposed to heavy metals, a change in the activity of this enzyme can be observed. In this experiment 3 groups of fish (European carp) were formed, 10 fish in each group: control group; 1-experimental group – fish were contained in water with 0.06 mg/l lead acetate concentration (upper threshold level than fishery waters permissible exposure limit according to the laws of the Russian Federation); 2-experimental group – fish were contained in water with 0.6 mg/l lead acetate concentration (10 times higher than fishery waters permissible exposure limit according to the laws of the Russian Federation). Exposure time was 4 hours. The blood samplings were taken from heart by syringe and placed in dry test tubes. After samplings centrifugation in non-hemolysed serum samples AP activity was assessed. The study revealed the decreasing trend of AP serum activity by 1.08% (21.89±0.24 IU/l) in 1-experimental group in compare with control group (22.13±0.78 IU/l). In case where the lead acetate concentration were 10 times higher than fishery waters permissible exposure limit were meaningful decreasing of AP activity (P ≤ 0.05) by 66.23% (17.42±0.63 IU/l) accordingly in 2-experimental group in compare with control group. In conclusion, a statistically significant decrease in the AP activity in fish blood serum was found after acetate concentration 10 times higher than the permissible exposure limit in fishery waters, according to the laws of the Russian Federation. We can suppose that the activity of this enzyme is the reason for the inhibitory effect of lead on liver tissue.

scholarly journals Evaluation of the effectiveness methods of initial pilots’ proffessional training to operate the aircraft equipped with electronic flight instrument systems

2018 ◽  
Vol 21 (5) ◽  
pp. 8-22
Author(s):  
S. G. Kosachevskiy ◽  
D. V. Aidarkin ◽  
D. V. Kachan
Keyword(s):  
Russian Federation ◽  
Professional Training ◽  
Flight Training ◽  
Simulator Training ◽  
Civil Aviation ◽  
Control Group ◽  
Series Of Experiments ◽  
The Russian Federation ◽  
New Generation ◽  
Experimental Group

Nowadays, educational institutions carrying out pilots’ training in the Russian Federation use completely new generation aircraft different with high degree of control automatisation (EFIS – Electronic Flight Instrument System). At the same time, the applied methods of flight training are based on studies carried out for aircraft with analog instruments, which does not allow to use new generation aircraft and simulators wide possibilities at  their full capacity. Therefore, there is a vital necessity of enhancing the method of pilots’ initial professional training that should contain teaching rational methods of distribution and switching visual attention. In 2017 in accordance with the Order of the Federal Air Transport Agency of the Russian Federation in the Ulyanovsk Institute of Civil Aviation a complex of research was carried out that allowed developing the method of initial pilots’ flight training on the aircraft equipped with EFIS. During the research, the oculometric research methods of cadets’ distribution and switching attention (the "eye-tracking" technology) were used, which allowed a deeper study of the piloting skills formation and their impact on pilot's operation with EFIS. To assess the effectiveness of the developed methods, two series of experiments involving cadets of Ulyanovsk Institute of Civil Aviation and its branch in Sasovo were conducted. Two types of simulators: L-410 and Diamond 40 NG were used for research purposes. In this article it is proposed to use the correlation coefficient and the Manhattan distance to assess the accuracy of maintaining flight parameters during cadet simulator training. According to the results of the first series of experiments, it was found out that the cadets under the experiment showed a lower level of flight training compared to the control group of cadets. However, after training the group under the experiment on the developed method, a level of flight preparation in the both groups became equal. Statistically significant differences of initial and final levels of flight training for the cadets of the experimental group were observed while cadets of a control group completed the series of experiments without any significant changes. The second series of experiments revealed that the lack of flight practice among the cadets of the experimental group did not prevent them from demonstrating a qualitative level of professional training when practicing on the flight simulator. Such a result was achieved due to the ground preparation of cadets on the developed method what, undoubtedly, indicates its effectiveness.

scholarly journals CLINICAL AND IMMUNOLOGICAL EFFECTIVENESS OF TREATMENT OF RAGWEED POLLINOSIS IN THE SAMARA REGION

2021 ◽  
Vol 23 (4) ◽  
pp. 921-926
Author(s):  
N. N. Zhukova ◽  
M. V. Manzhos ◽  
L. R. Khabibulina ◽  
E. Yu. Syrtsova
Keyword(s):  
Allergic Rhinitis ◽  
Russian Federation ◽  
Allergic Diseases ◽  
Ambrosia Artemisiifolia ◽  
Control Group ◽  
Ambrosia Trifida ◽  
Results Of Treatment ◽  
Amb A 1 ◽  
The Russian Federation ◽  
Time Changes

Allergic rhinitis and bronchial asthma are widespread respiratory allergic diseases. In some territories of the Russian Federation, the dominant cause of pollinosis is ragweed. The aim of the study was to evaluate the clinical and immunological efficacy of ASIT with the allergen Ambrosia artemisiifolia in patients sensitized to Ambrosia trifida in the Samara region. Patients with proven sensitization to Ambrosia trifida was held immunotherapy with Ambrosia artemisiifolia allergoid preseason. After treatment, patients had a decrease in the severity of symptoms of allergic rhinitis according to VAS (p = 0.00001), a decrease in the need for medications (p = 0.0003), as well as the need for corticosteroids against the background of therapy from 34.6% to 0% (p = 0.00001). In 8% of cases, the result of treatment was good, in 69% satisfactory, in 23% unsatisfactory. In the control group, there were no changes in the severity of symptoms (p = 0.858). Also, in the control group, the need for medications remained unchanged and 14.3% of patients continued to use corticosteroids.After ASIT, there was a decrease in the level of IL-4 (p = 0.002), and a decrease in the ratio of IL-4/ IL-10 (p = 0.0063); at the same time, changes in the level of other cytokines (IL-10; IFNγ) were statistically insignificant (p > 0.05). Before treatment, the levels of IL-4/ IL-10 in both groups were comparable, and after treatment, the differences became statistically significant (p = 0.031). We did not get a statistically significant change in the level of IgG4 Amb a 1 or IgG4 Amb trifida. There was no correlation between the level of individual cytokines and the results of treatment. As a result of the conducted ASIT, positive clinical and immunological results were obtained. In most patients, the disease has acquired a controlled course. At the same time, the lack of excellent and low number of good results of ASIT is probably due to the intraspecific allergenic properties of ragweed. 

ANALYSIS OF INNOVATIVE ACTIVITY OF ORGANIZATIONS OF THE RUSSIAN FEDERATION

2020 ◽  
Vol 3 (12) ◽  
pp. 99-103
Author(s):  
L. A. FEDOROVA ◽  
Keyword(s):  
Human Resources ◽  
Information And Communication Technologies ◽  
Russian Federation ◽  
Communication Technologies ◽  
Economic Effects ◽  
Activity Level ◽  
Innovative Development ◽  
Information And Communication ◽  
The Russian Federation ◽  
Science And Research

The article highlights the results of assessing the human resources potential and socio-economic effects of innovative development of the national economy of the Russian Federation for the period 2011–2019. The analysis of the indicators of the number of people employed in science and research, qualification and age structure, level of publication activity, level of development of information and communication technologies, etc. the conclusions regarding the level of achievement of target indicators of Strategy of innovative development of Russia up to 2020.

scholarly journals PSXIV-5 Results of external use of staphylococcal toxoid for the prevention of mastitis in cows

2019 ◽  
Vol 97 (Supplement_3) ◽  
pp. 315-315
Author(s):  
Dariya Kashtanova ◽  
Valerii G Skopichev ◽  
Flura Alistratova ◽  
Polina A Polistovskaia ◽  
Nataliya A Panova ◽  
...  
Keyword(s):  
Russian Federation ◽  
Economic Losses ◽  
Economic Damage ◽  
Control Group ◽  
Dry Period ◽  
North West ◽  
Lactating Cows ◽  
The North ◽  
The Russian Federation ◽  
The Cost

Abstract Mastitis in cows in the farms of the Russian Federation cause great economic damage. Economic losses include the cost of treating cows and the cost of recycled milk. In the farms of the North-West region of the Russian Federation mastitis occurs in 20–30% of lactating cows. An important task is to find effective ways to prevent mastitis. The task of our research included the study of the effectiveness of external use of staphylococcal toxoid. For the experiment, 2 experimental groups of dry cows of 10 animals each were formed. For the first group of cows, 5 days before the expected date of calving, an ointment containing staphylococcal toxoid and dimethyl sulfoxide was applied to the area of the milk mirror. Udder treatment was performed once a day for five days. Cows of the second group were immunized with Starvac® vaccine in accordance with the instructions, twice during the dry period. The third group of cows (n = 20) was the control. After calving, the concentration of immunoglobulins in colostrum was examined in all cows. In cows of the first group, the concentration of immunoglobulins G, M and A in colostrum was 8.59 ± 0.9; 1.1 ± 0.13 and 5.42 ± 0.9 g / l, respectively. The concentration of immunoglobulins in cows of the first group was higher, on average, by 1.3–2.0 times in comparison with cows that were given the Starvac vaccine. In comparison with the control group, the growth of all classes of immunoglobulins was determined by 2.2 - 5.2 times (P < 0.01). The results obtained showed high efficacy of external use of staphylococcal toxoid together with dimethisulfoxide.

scholarly journals The In vivo evaluation of anticryptosporidiosis activity of the methanolic extract of the plant Umbilicus rupestris

2019 ◽  
Vol 20 (12) ◽  
Author(s):  
AFAF BENHOUDA ◽  
DJAHIDA BENHOUDA ◽  
MASSINISSA YAHIA
Keyword(s):  
Methanolic Extract ◽  
Activity Level ◽  
Female Rats ◽  
Control Group ◽  
Histopathological Study ◽  
In Vivo Evaluation ◽  
Group I ◽  
Cryptosporidium Oocysts ◽  
Experimental Group

Abstract. Benhouda A, Benhouda D, Yahia M. 2019. In vivo evaluation of anticryptosporidiosis activity of the methanolic extract of the plant Umbilicus rupestris. Biodiversitas 20: 3478-3483. Umbilicus rupestris (Crassulaceae) is a medicinal plant used in general traditional medicine to cure inflammation and irritation of the skin. The present research is aimed to evaluate the antiparasitic activity of the methanolic extract of the plant URMeOH of U. rupestris against the Cryptosporidium infection in immunocompetent and immunosuppressed rats experimentally infected. Twenty-one female rats were divided into two groups. Control group (group I) and experimental group (Group II). The group I was further divided into three equal groups (normal group infected and immunosuppressed infected group). The experimental group was divided into two immunosuppressed and four equal groups and two immunocompetent infected. Cryptosporidium oocysts were isolated from bovine species stools and used to infect rats. Experimental subgroups received URMeOH two as dose 100mg/kg b.w. and 200 mg/kg b.w. and continued until 15 days. Two weeks after the administration of URMeOH, feces of rats were examined for the detection of Cryptosporidium oocysts by Ziehl-Neelsen and immunofluorescence techniques, the animals were sacrificed; their small intestines were processed and examined for the detection of pathological lesions after histopathological study. In addition, the activity of myeloperoxidase (MPO) was measured in sections of the jejunum. Concerned the results, we observed a statistically significant (P<0.001) increase in the number of oocysts of Cryptosporidium in the stool for sub infected immunosuppressed groups and an increase of MPO activity compared to the corresponding subgroups immunocompetent subgroups. The URMeOH could remove Cryptosporidium oocysts from feces and intestinal sections subgroup infected immunocompetent rats receiving URMeOH. Moreover, the oocysts were significantly reduced in all other subgroups experimental infected compared to infected control subgroups. Intestinal sections in all subgroups received URMeOH revealed a more or less normal architecture. In addition, the reduction of MPO activity level was also detected in all experimental subgroups.

scholarly journals MIGRATION AND INVESTMENT ACTIVITY IN THE REGIONS OF THE SIBERIAN FEDERAL DISTRICT OF THE RUSSIAN FEDERATION

2020 ◽  
Vol 12 (1) ◽  
Author(s):  
Kseniya PARFENOVA ◽  
Olga GUROVA
Keyword(s):  
Economic Development ◽  
Russian Federation ◽  
Federal District ◽  
Activity Level ◽  
Siberian Federal District ◽  
Investment Activity ◽  
Irkutsk Region ◽  
Social And Economic Development ◽  
Visual Reference ◽  
The Russian Federation

This article analyses the migration and investment activity processes in constituent entities of the Russian Federation located in the Siberian Federal District (SFD). The research was performed on the basis of official statistical data with geographical visual reference. The study was performed in the mono-specialized cities of SFD regions on the basis of official statistics within the context of the creation of Priority Social and Economic Development Areas (PSEDA). A negative migration balance is registered in SFD regions and mono-specialized settlements. Investment activity in SFD territories, their regional potentials and risks were analyzed. Krasnoyarsk Territory, Tomsk Region, Irkutsk Region, Novosibirsk Region, and Kemerovo Region stand out among the SFD regions by their investment activity level and social and economic development. At the current stage of social and economic development, Priority Social and Economic Development Areas can be successfully created in the regions which are the most developed both industrially and innovatively and in the mono-specialized settlements with the most favorable start conditions.

scholarly journals Assessing the decoupling of economic growth from environmental impacts in industrial regions of the Russian Federation: an impact decoupling approach

2021 ◽  
Vol 116 ◽  
pp. 00026
Author(s):  
Anna Pepelyaeva ◽  
Aleksandra Krutova
Keyword(s):  
Russian Federation ◽  
Growth Rates ◽  
Economic Activity ◽  
Activity Level ◽  
Sustainable Growth ◽  
Pollutant Emissions ◽  
The Russian Federation ◽  
Industrial Regions ◽  
Per Capita ◽  
Decoupling Coefficient

The article analyzes the dynamics of the economic activity level in Russian industrial regions and its impact on the main environmental elements (water, air and soil) by calculating the decoupling coefficient. In the study a hierarchical cluster analysis was carried out, resulted in the formation of seven regional clusters, five of which had a significant share of the industrial sector in the gross value-added structure. Decoupling coefficients were calculated for 64 constituent entities of the Russian Federation belonging to different types of industrial clusters. The ecological and economic balance assessment of industrial regions growth showed the decoupling effect presence for most of them. The movement towards environmentally sustainable growth is more evident in the regions of the industrially diversified cluster. The analysis of three decoupling coefficients showed that in the vast majority of industrial regions the growth rates of pollutant emissions into the atmosphere and the growth rates of polluted wastewater discharges into surface water change at a lower rate than per capita GRP, which indicates a “green trend” in the region’s economic activity. However, the decoupling coefficient, showing the dynamics ratio of production and consumption waste and GRP per capita, has negative value in 37.5% of regions. The most problematic one in terms of this indicator was the Trade and manufacturing cluster, including 21 constituent entities of the Russian Federation. According to the authors, it may be related to the trade sector of these regions.

scholarly journals The Effect Of Exercise Consultation Program On Physical Activity Level In Elderly People At Darussalam Community Health Center,North Sumatra

2020 ◽  
Vol 8 (3) ◽  
pp. 417
Author(s):  
Rinco Siregar ◽  
Rumondang Gultom ◽  
Frida Liharis Saragih
Keyword(s):  
Physical Activity ◽  
Community Health ◽  
Health Center ◽  
Elderly People ◽  
Physical Activity Level ◽  
Community Health Center ◽  
Activity Level ◽  
Control Group ◽  
Significant Difference ◽  
Experimental Group

Background : It is importan to promote physical activity for elderly people, but to improve level of physical activity of elderly people is a challange for health workers. Purpose : The purpose of this quasi- experimental study was to determine the effect of exercise consultation program by using Transtheoretical Model (TTM) approach on physical activity level in elderly people at Darussalam’s Community Health Center. Research method :The two comparison groups for this study were purposively designed for pre-test/post-test procedures; sixty elderly people at Darussalam’s Community Health Center with randomly allocated into the experimental (30 people) and the control (30 people) groups. While the experimental group received the exercise consultation for 8 weeks, the control group received routine care. Physical Activity Scale for Elderly (PASE) was use to assess the the physical activity of elderly. Comparative assessments on differences in level of physical activity both within group using Wilcoxson macthed-pairs and between group using Mann Withney U Test. Results : The results of this study found that after receiving exercise consultation program, level of physical activity in elderly people significantly increased in the experimental group (P < 0.05), but there was no significant difference in the control group. Between groups,  level of physical activity significantly increased after receiving the exercise consultation while no change was found among those who did not (P < 0.05).Conclusion: The exercise consultation program by using TTM approach could increase level of physical activity of elderly people.

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