scholarly journals Correlation of the rumen fluid microbiome and the average daily gain with a dietary supplementation of Allium mongolicum Regel extracts in sheep1

2019 ◽  
Vol 97 (7) ◽  
pp. 2865-2877 ◽  
Author(s):  
Hongxi Du ◽  
Khas Erdene ◽  
Shengyang Chen ◽  
Saruli Qi ◽  
Zhibi Bao ◽  
...  
Keyword(s):  
Rumen Fluid ◽  
Average Daily Gain ◽  
Basal Diet ◽  
Experimental Period ◽  
Control Group ◽  
Illumina Hiseq ◽  
Rumen Microbiome ◽  
Microbiome Profile ◽  
Dietary Treatments ◽  
Powder Extract

Abstract Plant extracts can affect the rumen microbiome and ADG in ruminants, and studies of the association between the rumen microbiome and ADG provide information applicable to improving ruminant growth performance. The objectives were to investigate the effects of Allium mongolicum Regel extracts on the rumen microbiome and ADG and their association in sheep. Forty healthy, male, small-tailed Han sheep (6 mo, 34 ± 3.5 kg body weight) were randomly assigned to 1 of the following 4 dietary treatments: basal diet as control group (CK, n = 10), basal diet supplemented with 3.4 g·sheep−1·d−1A. mongolicum Regel powder extract as PAM group (PAM, n = 10), basal diet supplemented with 10 g·sheep−1·d−1A. mongolicum Regel powder as AM group (AM, n = 10), and basal diet supplemented with 10 g·sheep−1·d−1A. mongolicum Regel powder extract residue as RAM group (RAM, n = 10). The ADG for individual sheep was calculated using the sum of the ADGs observed during the experimental period divided by the number of days in the experimental period. At the end of the experiment, sheep were randomly selected from each treatment for slaughter (n = 6), and the rumen fluids were collected and stored immediately at −80 °C. Illumina HiSeq was subsequently used to investigate the changes in the rumen microbiome profile, and the associations with ADG were analyzed by Spearman correlation coefficient analysis. The results demonstrated that, compared with that in CK group, the ADG in AM and RAM significantly increased (P = 0.0171). The abundances of Tenericutes and Mollicutes ([ρ] = 0.5021, P = 0.0124) were positively correlated with ADG. Within Mollicutes, the abundances of Anaeroplasmatales ([ρ] = 0.5458, P = 0.0058) and Anaeroplasmataceae ([ρ] = 0.5458, P = 0.0058) were positively correlated with ADG. The main negatively correlated bacteria were Saccharibacteria ([ρ] = −0.4762, P = 0.0187) and Betaproteobacteria ([ρ] = −0.5669, P = 0.039). Although Anaeroplasmatales and Anaeroplasmataceae were positively correlated with ADG, Saccharibacteria and Betaproteobacteria were negatively correlated with ADG. In conclusion, supplementation with A. mongolicum Regel powder and extracts will influence the rumen microbiome and increase the ADG.

scholarly journals Dietary purslane (Portulaca oleracea L.) promotes the growth performance of broilers by modulation of gut microbiota

AMB Express ◽  
2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Cong Wang ◽  
Qing Liu ◽  
Fengchun Ye ◽  
Hongbo Tang ◽  
Yanpeng Xiong ◽  
...  
Keyword(s):  
Gut Microbiota ◽  
Growth Performance ◽  
Basal Diet ◽  
Scientific Basis ◽  
Control Group ◽  
Good Growth ◽  
Feed Conversion ◽  
Illumina Hiseq ◽  
Treatment Groups ◽  
Dietary Treatments

AbstractPurslane is a widespread wild vegetable with both medicinal and edible properties. It is highly appreciated for its high nutritional value and is also considered as a high-quality feed resource for livestock and poultry. In this study, Sanhuang broilers were used to investigate the effect of feeding purslane diets on the growth performance in broilers and their gut microbiota. A total of 48 birds with good growth and uniform weight were selected and randomly allocated to four treatment groups A (control), B, C and D. Dietary treatments were fed with basal diet without purslane and diets containing 1%, 2% and 3% purslane. The 16S rDNA was amplified by PCR and sequenced using the Illumina HiSeq platform to analyze the composition and diversity of gut microbiota in the four sets of samples. The results showed that dietary inclusion of 2% and 3% purslane could significantly improve the growth performance and reduce the feed conversion ratio. Microbial diversity analysis indicated that the composition of gut microbiota of Sanhuang broilers mainly included Gallibacterium, Bacteroides and Escherichia-Shigella, etc. As the content of purslane was increased, the abundance of Lactobacillus increased significantly, and Escherichia-Shigella decreased. LEfSe analysis revealed that Bacteroides_caecigallinarum, Lachnospiraceae, Lactobacillales and Firmicutes had significant differences compared with the control group. PICRUSt analysis revealed bacteria mainly enriched in carbohydrate metabolism pathway due to the additon of purslane in the diet. These results suggest that the addition of purslane to feed could increase the abundance of Lactobacillus in intestine, modulate the environment of gut microbiota and promote the metabolism of carbohydrates to improve its growth performance. This study indicates that the effect of purslane on the growth-promoting performance of broilers might depend on its modulation on gut microbiota, so as to provide a certain scientific basis for the application of purslane in the feed industry.

scholarly journals Garlic skin induced shifts in the rumen microbiome and metabolome of fattening sheep

2020 ◽  
Author(s):  
Wen Zhu ◽  
Zhen Su ◽  
Wei Xu ◽  
Hanxue Sun ◽  
Jinfeng Gao ◽  
...  
Keyword(s):  
Growth Performance ◽  
Average Daily Gain ◽  
Basal Diet ◽  
Diversity Indices ◽  
Rumen Fermentation ◽  
Control Group ◽  
Rumen Microbiome ◽  
Volatile Fatty Acid Concentration ◽  
Α Diversity ◽  
Daily Gain

Abstract Background Garlic and its constituents exhibit activities on modifying rumen fermentation and improving growth performance. As a by-product of garlic processing, garlic skin contains similar bioactive components as garlic bulb. However, studies in ruminants using garlic skin are scarce. This study was conducted to investigate the effects of garlic skin supplementation on rumen fermentation characterizes, growth performance, ruminal microbes and metabolites in ruminants. Twelve Hu lambs were randomly assigned into one of two treatments: basal diet (CON) or basal diet supplemented with 80 g/kg DM of gallic skin (GAS). The experiment lasted for10 weeks, with the first 2 weeks for adaptation. Results The results revealed that the average daily gain and volatile fatty acid concentration were higher (P < 0.05) in lambs fed GAS than that in the control group. Garlic skin supplementation did not significantly (P > 0.10) affect the α-diversity indices. Increased (P < 0.05) abundances of Prevotella, Bulleidia, Howardella, Methanosphaera but a decreased (P < 0.05) abundance of Fretibacterium were observed in GAS-fed lambs. In addition, the garlic skin supplementation favorably regulated (P < 0.05) pyrimidine metabolism, purine metabolism, vitamin B6 and B1 metabolism. Moreover, high correlations were observed between fluctuant rumen microbiota and metabolites. Conclusions Supplementation of garlic skin improved the growth performance of sheep by modifying rumen fermentation through inducing shifts in the rumen microbiome and metabolome.

scholarly journals Preliminary assessment of probiotic Bacillus subtilis C-3102 in feces: evaluation of their survival after oral supplementation in goats

2020 ◽  
Vol 19 (2) ◽  
pp. 153-159
Author(s):  
Watcharapong Choonkham ◽  
◽  
Axel van Ruitenbeek ◽  
Jan Thomas Schonewille ◽  
Witaya Suriyasathaporn ◽  
...  
Keyword(s):  
Body Weight ◽  
Bacillus Subtilis ◽  
Rumen Fluid ◽  
Basal Diet ◽  
Experimental Period ◽  
Future Research ◽  
Adaptation Period ◽  
Fecal Samples ◽  
Oral Supplementation ◽  
Dietary Treatments

The objective of the study was to assess the presence of Bacillus subtilis C-3102 in feces after oral supplementation in goats. Six, rumen-fistulated, 3.5 year-old, non-lactating female Saanen goats (average initial body weight of 65 ± 8 kg) were assigned to two treatments 1) a basal diet (CON), and 2) a basal diet supplemented with B. subtilis C-3102 probiotic product (BS) in a cross-over design. Each experimental period lasted 21 days with was preceded by a 20 days adaptation period. On the last day of each experimental period, rumen fluid and fecal samples were collected. Body weights were recorded weekly throughout the experiment. Body weight and rumen pH were found to be similar between dietary treatments. The goats that received BS had higher numbers of B. subtilis C-3102 in fecal samples than CON. It was therefore concluded that supplemental B. subtilis C-3102 met an important precondition of probiotics in that they can survive the passage through the gastrointestinal tract without evident adverse effects. The current result provides a solid basis for future research involving any effects after supplementing probiotic B. subtilis in goats.

Milk production responses to turnips fed to dairy cows in mid lactation

1998 ◽  
Vol 38 (2) ◽  
pp. 117 ◽  
Author(s):  
P. J. Moate ◽  
D. E. Dalley ◽  
K. Martin ◽  
C. Grainger
Keyword(s):  
Dairy Cows ◽  
Milk Production ◽  
Dry Matter ◽  
Rumen Fluid ◽  
Basal Diet ◽  
Milk Composition ◽  
Control Group ◽  
Physical Form ◽  
Yield Responses ◽  
Dietary Treatments

Summary. Recently, many dairy farmers in Victoria have grown turnips as a summer fodder crop for lactating dairy cows. This paper reports on a 1 month experiment in which cows were individually fed indoors, and milk yield responses to feeding combinations of turnips and barley were measured. Forty cows in mid lactation were evenly allocated to 5 treatment groups. The control group were offered a basal diet of pasture hay and pasture silage which is similar to that available on many Victorian dairy farms during summer. Cows in the other 4 groups were offered the basal diet and various combinations of turnips and/or barley. Marginal milk responses from barley, turnips and a 50 : 50 mixture of turnips and barley were 0.62, 0.49 and 0.59 L/kg dry matter of supplement eaten. Milk composition of all groups was similar. Despite diets differing greatly in the proportions and physical form of dietary constituents, the mean milk production of each group was accurately predicted by their mean dry matter intake. Measurement of plasma minerals (calcium, magnesium, sodium, potassium and phosphorus) and rumen fluid pH showed that dietary treatments caused some significant (P<0.05) differences but all values were within the normal range. In this experiment, the milk production responses from barley, turnips and combinations of barley and turnips were similar. Since the cost of turnips is generally about half that of barley, it is concluded that turnips are an economic alternative to barley as a feed supplement for summer milk production.

scholarly journals Garlic Skin Induced Shifts in the Rumen Microbiome and Metabolome of Fattening Sheep

2020 ◽  
Author(s):  
WEN Zhu ◽  
Zhen Su ◽  
Wei Xu ◽  
Hanxue Sun ◽  
Jinfeng Gao ◽  
...  
Keyword(s):  
Growth Performance ◽  
Average Daily Gain ◽  
Basal Diet ◽  
Diversity Indices ◽  
Rumen Fermentation ◽  
Control Group ◽  
Rumen Microbiome ◽  
Volatile Fatty Acid Concentration ◽  
Α Diversity ◽  
Daily Gain

Abstract Background: Garlic and its constituents exhibit activities on modifying rumen fermentation and improving growth performance. As a by-product of garlic processing, garlic skin contains similar bioactive components as garlic bulb. However, studies in ruminants using garlic skin are scarce. This experiment was conducted to investigate the effects of garlic skin supplementation on rumen fermentation characterizes, growth performance, and involved mechanism in ruminants. Twelve Hu lambs were randomly assigned into one of two treatments: basal diet (CON) or basal diet supplemented with 80 g/kg DM of gallic skin (GAS). The experiment lasted for10 weeks, with the first 2 weeks for adaptation. Results: The results revealed that the average daily gain and volatile fatty acid concentration were higher (P < 0.05) in lambs fed GAS than that in the control group. Garlic skin supplementation did not significantly (P > 0.10) affect the α-diversity indices. Increased (P < 0.05) abundances of Prevotella, Bulleidia, Howardella, Methanosphaera but a decreased (P < 0.05) abundance of Fretibacterium were observed in GAS-fed lambs. Besides, the garlic skin supplementation favorably regulated (P < 0.05) pyrimidine metabolism, purine metabolism, vitamin B6 and B1 metabolism. Moreover, high correlations were observed between fluctuant rumen microbiota and metabolites. Conclusions: Supplementation of garlic skin improved the growth performance of sheep by modifying rumen fermentation through inducing shifts in the rumen microbiome and metabolome.

scholarly journals Dietary Purslane (Portulaca Oleracea L.) Promotes the Growth Performance of Broilers by Modulation of Gut Microbiota

2020 ◽  
Author(s):  
Cong Wang ◽  
Qing Liu ◽  
Fengchun Ye ◽  
Hongbo Tang ◽  
Yanpeng Xiong ◽  
...  
Keyword(s):  
Gut Microbiota ◽  
Growth Performance ◽  
Basal Diet ◽  
Scientific Basis ◽  
Control Group ◽  
Good Growth ◽  
Feed Conversion ◽  
Illumina Hiseq ◽  
Treatment Groups ◽  
Dietary Treatments

Abstract Purslane is a widespread wild vegetable with both medicinal and edible properties. It is highly appreciated for its high nutritional value and is also considered as a high-quality feed resource for livestock and poultry. In this study, Sanhuang broilers were used to investigate the effect of feeding purslane diets on the growth performance in broilers and their gut microbiota. A total of 48 birds with good growth and uniform weight were selected and randomly allocated to four treatment groups A (control), B, C and D. Dietary treatments were fed with basal diet without purslane and diets containing 1%, 2% and 3% purslane. The 16S rDNA was amplified by PCR and sequenced using the Illumina HiSeq platform to analyze the composition and diversity of gut microbiota in the four sets of samples. The results showed that dietary inclusion of 2% and 3% purslane could significantly improve the growth performance and reduce the feed conversion ratio. Microbial diversity analysis indicated that the composition of gut microbiota of Sanhuang broilers mainly included Gallibacterium, Bacteroides and Escherichia-Shigella, etc. As the content of purslane was increased, the abundance of Lactobacillus increased significantly, and Escherichia-Shigella decreased. LEfSe analysis revealed that Bacteroides_caecigallinarum, Lachnospiraceae, Lactobacillales and Firmicutes had significant differences compared with the control group. PICRUSt analysis revealed bacteria mainly enriched in carbohydrate metabolism pathway due to the additon of purslane in the diet. These results suggest that the addition of purslane to feed could increase the abundance of Lactobacillus in intestine, modulate the environment of gut microbiota and promote the metabolism of carbohydrates to improve its growth performance. This study indicates that the effect of purslane on the growth-promoting performance of broilers might depend on its modulation on gut microbiota, so as to provide a certain scientific basis for the application of purslane in the feed industry.

Supplementation of Diet With Microbial And Thermo-Stable 6-Phytase With Recommended And Deficient CA and P Level on Broiler Performance

2021 ◽  
Vol 19 (1) ◽  
pp. 211-222
Author(s):  
MT Islam ◽  
M Habib ◽  
AJM Ferdaus ◽  
MBR Mollah ◽  
MS Ali
Keyword(s):  
Body Weight ◽  
Basal Diet ◽  
Experimental Period ◽  
Feed Consumption ◽  
Control Group ◽  
Broiler Chicks ◽  
Feed Conversion ◽  
Broiler Performance ◽  
Microbial Phytase ◽  
Dietary Treatments

An experiment was carried out to investigate the performances with 1080 male Cobb-500 broiler chicks using different levels of a microbial phytase in plantbased diet. Six dietary treatments were formulated as follows; the first 3 diets were balanced with Ca and Av. P according to the recommendation of Cobb-500 served as basal diet without phytase enzyme (T1), basal diet with 100g phytase ton-1 feed (T2) and basal diet with 200g phytase ton-1 feed (T3), while the last 3 diets were deficient in Ca and Av. P and containing no phytase enzyme (T4), diet with 100g phytase/ton feed (T5) and diet with 200g phytase ton-1 feed (T6). All 6 treatments were replicated 6 times. Body weight, feed consumption and mortality of broilers were recorded weekly. The highest body weight was found in T3 with 200g phytase ton-1 feed with recommended Ca and Av. P level in the diet, followed by T6 with 200g phytase/ton feed with deficient in Ca and Av. P level in the diet (P<0.05). The lowest weight was found in T1 without phytase with recommended Ca and Av. P level in the diet. The feed consumption, feed conversion ratio and mortality of the birds during 35 days of experimental period did not differ significantly (P<0.05). The meat yield characteristics at 35 days of age were non-significant among different treatments (P>0.05). Significantly lower value of total ash (%), Ca (%) and total P (%) of tibia was found in control group (T1) and T4. The results of this experiment revealed that phytase supplementation at 200g ton-1 of feed improved broiler performances at either recommended or deficient level of Ca and Av. P. SAARC J. Agric., 19(1): 211-222 (2021)

scholarly journals Effects of Dietary Inclusion of Dry Hydrastis canadensis on Laying Performance, Egg Quality, Serum Biochemical Parameters and Cecal Microbiota in Laying Hens

Animals ◽  
2021 ◽  
Vol 11 (5) ◽  
pp. 1381
Author(s):  
Tzuen-Rong J Tzeng ◽  
Tzu-Yu Liu ◽  
Chiao-Wei Lin ◽  
Pei-En Chang ◽  
Pei-Xin Liao ◽  
...  
Keyword(s):  
Biochemical Parameters ◽  
Egg Production ◽  
Laying Hens ◽  
Basal Diet ◽  
Response To Treatment ◽  
Control Group ◽  
Hydrastis Canadensis ◽  
Laying Performance ◽  
Cecal Microbiota ◽  
Dietary Treatments

Alternative growth promoters are able to not only effectively replace the traditional use of antibiotics but also provide additional health benefits for livestock and reduce food safety concerns. This study investigated the effects of dry Hydrastis canadensis on the laying performance and fecal microbial community of laying hens. Twenty-four Lohmann (LSL, white layer strain) hens were reared from 40 to 48 weeks of age and randomly allotted to four dietary treatments (six birds/treatment). The dietary treatments comprised a basal diet with no treatment as control, a basal diet plus 0.6% powder of dry Hydrastis canadensis roots (R) or leaves (L), and a basal diet plus 0.6% powder of a mixture of dry Hydrastis canadensis roots and leaves (1:1, LR). No mortality was observed in the whole experimental period. The results indicated that albumen height in the LR group was significantly greater than that in the control group. The diet supplemented with Hydrastis canadensis had no significant effects on egg production rate, egg weight, eggshell strength, eggshell thickness, Haugh unit, or yolk height during the whole experimental phase. However, principal coordinate analysis, comparative heat map analysis, and cluster dendrogram analysis of cecal microbiota showed distinct clusters among the groups treated with Hydrastis canadensis and the control group. Regarding blood biochemical parameters, serum cholesterol levels were significantly lower in all Hydrastis canadensis-treated groups compared with those in the control group. Moreover, serum low-density lipoprotein levels were lower in hens supplemented with the leaf of Hydrastis canadensis. The abundances of the phyla Fusobacteria and Kiritimatiellaeota were increased (p < 0.05) in laying hens fed with 0.6% Hydrastis canadensis leaves, whereas the abundance of the phylum Firmicutes in cecum digesta decreased in response to treatment with Hydrastis canadensis roots and leaves. The relative abundance of the Fusobacterium genus was higher in the LR group compared with that in the control. On the contrary, we found a different trend in the Synergistes genus. The potential influences of these microbiota on the performance of laying hens were discussed. The results demonstrate that Hydrastis canadensis can improve the egg albumen height and modulate the cecum digesta microbiota composition of laying hens.

scholarly journals Effects of Dietary Supplementation of gEGF on the Growth Performance and Immunity of Broilers

Animals ◽  
2021 ◽  
Vol 11 (5) ◽  
pp. 1394
Author(s):  
Jianyong Zhou ◽  
Jingyi Yao ◽  
Luhong Bai ◽  
Chuansong Sun ◽  
Jianjun Lu
Keyword(s):  
Antioxidant Capacity ◽  
Growth Performance ◽  
Crude Extract ◽  
Serum Alkaline Phosphatase ◽  
Average Daily Gain ◽  
Basal Diet ◽  
Control Group ◽  
Serum Samples ◽  
Chicken Embryos ◽  
Growth Of Animals

EGF has been shown to stimulate the growth of animals. In this study, the content of EGF in chicken embryos (gallus EGF, gEGF) aged from 1 to 20 days of incubation were determined by ELISA kit, and the 5-day-old chicken embryos with the highest content of 5593 pg/g were selected to make gEGF crude extracts. A total of 1500 1-day-old Xianju chickens were randomly divided into five groups with six replicates of 50 chickens each. The control group was fed a basal diet, and other treatment diets were supplemented with 4, 8, 16 and 32 ng/kg gEGF crude extract, respectively. The experiment lasted for 30 days. Chicks were harvested at the end of the experiment, and liver, spleen, thymus, bursa and serum samples were collected. Results showed that average daily gain (ADG) and average daily feed intake (ADFI) of 16 ng/kg group were higher than those in the control group (p < 0.05). The serum uric acid (UA) of the 16 ng/kg group was reduced (p < 0.01), and the serum alkaline phosphatase (AKP) of the 16 ng/kg group increased (p < 0.01). The gEGF extract also increased chick’s antioxidant capacity, decreased malondialdehyde (MDA) and increased catalase (CAT) in the liver and serum of 16 ng/kg groups in compared to the control group (p < 0.01). Furthermore, immunity was improved by the addition of gEGF to broiler diets. The serum immunoglobin A (IgA) content of 8 and 16 ng/kg groups and the serum immunoglobin M (IgM) content of 4 and 8 ng/kg groups were increased (p < 0.05) compared to the control group. The bursa index of each experimental group was higher than the control group (p < 0.01). These findings demonstrate that the crude extract of gEGF prepared in this experiment could improve the growth performance, antioxidant capacity and immunity of broilers.

PSVII-23 Experimental evaluation of the effectiveness of the Quercus cortex extract in the carp feeding system

2021 ◽  
Vol 99 (Supplement_3) ◽  
pp. 339-339
Author(s):  
Mariya Miroshnikova ◽  
Elena Miroshnikova ◽  
Alexey Sizentsov ◽  
Azamat Arinzhanov ◽  
Yuliya Kilyakova
Keyword(s):  
Calcium Content ◽  
Fish Farming ◽  
Basal Diet ◽  
Experimental Period ◽  
Atomic Emission ◽  
Essential Elements ◽  
Control Group ◽  
Metagenomic Sequencing ◽  
Microbial Profile ◽  
Combined Feed

Abstract One of the most promising ways to improve the effectiveness of fish farming is the use of phytobiotics in the diet of fish. On this basis, we set the aim to evaluate the effectiveness of the Quercus cortex extract in the concentration of 1 mg/kg on biological features and the productivity of carp. The object of the research was yearling carp grown in the conditions of Orenburgskiy Osetr LLC. Two groups (n = 20) were formed by the method of analogs to conduct the research. After the adaption period (7 days), the groups came to the experimental period (35 days). The feed KRK-110–1 produced by PJSC Orenburg Combined-Feed Plant was used as the basal diet. The live fish were monitored weekly by individual weighing during the accounting period. The elemental composition was determined by the method of atomic emission and mass spectrometry (ICP-AES and ICP-MS). The microflora analysis was carried out by the method of metagenomic sequencing. The use of the Quercus cortex extract in the diet in the studied concentration increased body weight by 15.1% (P ≤ 0.05) compared to the control group. There was experimentally revealed a stimulating effect on the population growth of individual representatives of the microbiome (Luteolibacter, Lactococcus) (P ≤ 0.05) without significantly changing the overall picture of the microbial profile, which, in our view, affects the metabolic processes, in particular, by stimulating the formation of biologically available forms of essential elements and their subsequent accumulation in the tissues of the studied fish. Thus, the experimental group found: (against the background of an increase in the total mineralization (ash residue) by 17.95 % (P ≤ 0.05)) the calcium content increased by 133.9% (P ≤ 0.05), phosphorus by 83% (P ≤ 0.05), iron by 337.7% (P ≤ 0.05), respectively, in comparison with the control group.

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