PSVII-23 Experimental evaluation of the effectiveness of the Quercus cortex extract in the carp feeding system

2021 ◽  
Vol 99 (Supplement_3) ◽  
pp. 339-339
Author(s):  
Mariya Miroshnikova ◽  
Elena Miroshnikova ◽  
Alexey Sizentsov ◽  
Azamat Arinzhanov ◽  
Yuliya Kilyakova
Keyword(s):  
Calcium Content ◽  
Fish Farming ◽  
Basal Diet ◽  
Experimental Period ◽  
Atomic Emission ◽  
Essential Elements ◽  
Control Group ◽  
Metagenomic Sequencing ◽  
Microbial Profile ◽  
Combined Feed

Abstract One of the most promising ways to improve the effectiveness of fish farming is the use of phytobiotics in the diet of fish. On this basis, we set the aim to evaluate the effectiveness of the Quercus cortex extract in the concentration of 1 mg/kg on biological features and the productivity of carp. The object of the research was yearling carp grown in the conditions of Orenburgskiy Osetr LLC. Two groups (n = 20) were formed by the method of analogs to conduct the research. After the adaption period (7 days), the groups came to the experimental period (35 days). The feed KRK-110–1 produced by PJSC Orenburg Combined-Feed Plant was used as the basal diet. The live fish were monitored weekly by individual weighing during the accounting period. The elemental composition was determined by the method of atomic emission and mass spectrometry (ICP-AES and ICP-MS). The microflora analysis was carried out by the method of metagenomic sequencing. The use of the Quercus cortex extract in the diet in the studied concentration increased body weight by 15.1% (P ≤ 0.05) compared to the control group. There was experimentally revealed a stimulating effect on the population growth of individual representatives of the microbiome (Luteolibacter, Lactococcus) (P ≤ 0.05) without significantly changing the overall picture of the microbial profile, which, in our view, affects the metabolic processes, in particular, by stimulating the formation of biologically available forms of essential elements and their subsequent accumulation in the tissues of the studied fish. Thus, the experimental group found: (against the background of an increase in the total mineralization (ash residue) by 17.95 % (P ≤ 0.05)) the calcium content increased by 133.9% (P ≤ 0.05), phosphorus by 83% (P ≤ 0.05), iron by 337.7% (P ≤ 0.05), respectively, in comparison with the control group.

Effects of Compound Probiotics on Growth Performance, Intestinal Microflora and Immune Function of Broilers

2014 ◽  
Vol 997 ◽  
pp. 178-184
Author(s):  
Hong Ling Wang ◽  
Yu Hong Yang ◽  
Jia Qing Wang ◽  
Zhuo Wang ◽  
Shuang Ma
Keyword(s):  
Immune Function ◽  
Growth Performance ◽  
Broiler Chickens ◽  
Intestinal Microflora ◽  
Basal Diet ◽  
Experimental Period ◽  
Control Group ◽  
Group Iii ◽  
Serum Biochemical ◽  
Anti Body

This study aimed to investigate the efficacy of compound probiotics on broiler performance, intestinal microflora, immune function and serum biochemical indicators. Four hundred one-d-old female broiler chickens were randomly divided into 4 groups with 4 replicates in each group and 25 chickens per replication. The chickens in groupI(control group) were fed a basal diet, experimental groups II, III and IVwere fed the basal diet supplemented with 0.1%, 0.2% and 0.4% compound probiotics. The results of this work were as follows: (1) during the experimental period, compared with control group, the average daily in group III was significantly increased (P<0.05), the feed/gain and average daily feed intake were significantly lower (P<0.05); (2) at the age of 14 and 28 days, the number of cecal Lactic acid in group II and III were significantly higher than the control (P<0.05) and the content of E.coli has the opposite results. (3) the anti-body titer in group III was the highest, and significantly higher compared to the control (P<0.05), but there were no significantly differences between group IV and control group (P>0.05). (4) serum biochemical indexes of broilers in group III and IVwere significantly increased than that in control group (P<0.05). In conclusion, the supplementation of 0.2% compound probiotics could promote the growth performance, improve the cecal mico-ecosystem, enhance the anti-body titer and biochemical indexes in serum.

scholarly journals Effect of the supplementation linseed oil, inulin and horse chestnut into a high fat diet on the fatty acid profile of pigs

2016 ◽  
Vol 46 (11) ◽  
pp. 1992-1997
Author(s):  
Matej Brestenský ◽  
Soňa Nitrayová ◽  
Peter Patráš ◽  
Jaroslav Heger ◽  
Jozef Nitray
Keyword(s):  
Linolenic Acid ◽  
High Fat Diet ◽  
Linseed Oil ◽  
Basal Diet ◽  
Experimental Period ◽  
Control Group ◽  
Horse Chestnut ◽  
Adaptation Period ◽  
High Fat ◽  
Lower Ratio

ABSTRACT: In the present study it was evaluated the effect of the addition of linseed oil, inulin and horse chestnut added to a high fat (HF) diet on the content of fatty acids (FAs) in musculuss longissimus dorsi (MLD) of pigs. A 5d with adaptation period was followed by a 70 d experimental period, during which the pigs were fed with a HF basal diet. The HF basal diet which served as a control (group CG) was supplemented either with linseed oil (group LG) or with inulin and horse chestnut (group IG). All of the pigs were slaughtered at the end of the experiment and samples of MLD were taken for FA analysis. The concentration of α-linolenic acid in MLD of the LG group was 58 % and 61 % higher (P˂0.05) compared to CG and IG groups, respectively. The content of eicosapentaenoic acid (EPA) was 0.03 and docosahexaenoic acid (DHA) 0.07 in LG treatment. These FAs were not detected in CG and IG. The ratio of MUFA and PUFA n-6/n-3 in the MLD was the lowest (P˂0.05) in the LG (8.84) compared to CG (14.07) or IG (14.74) groups, representing a difference of 31.2%. The n-3/saturated FA ratio was highest (P˂0.05) in LG group (0.04) when compared to CG and IG groups (0.02). The supplementation of linseed oil, into the HF diet resulted in a higher concentration of α-linolenic acid, EPA, DHA and lower ratio of n-6/n-3 FA in MLD. Inulin and horse chestnut had no effect on FA profile of MLD.

scholarly journals Dietary Inulin Supplementation Modulates Short-Chain Fatty Acid Levels and Cecum Microbiota Composition and Function in Chickens Infected With Salmonella

2020 ◽  
Vol 11 ◽  
Author(s):  
Jiao Song ◽  
Qinghe Li ◽  
Nadia Everaert ◽  
Ranran Liu ◽  
Maiqing Zheng ◽  
...  
Keyword(s):  
Basal Diet ◽  
Short Chain Fatty Acids ◽  
Nucleotide Metabolism ◽  
Short Chain ◽  
Control Group ◽  
Sequencing Analysis ◽  
Metagenomic Sequencing ◽  
Microbiome Composition ◽  
And Function ◽  
Functional Profiles

The current study investigated the effects of inulin on the gut microbiota, microbiome functions, and short-chain fatty acids (SCFAs) levels in specific pathogen-free (SPF) chickens infected with Salmonella enteritidis (SE). SPF Arbor Acres chickens (n = 240, 1-day-old) were divided into four groups: a control group (CON) fed a basal diet without inulin supplementation or SE infection, and three groups fed a basal diet supplemented with inulin 0, 0.5, and 1% (SE, 0.5%InSE, 1%InSE, respectively) up to 28-days-old, followed by SE challenge at 28 days of age. Cecal SCFA contents and microbiome composition and function were analyzed at 1-day post-infection. The results showed that SE infection significantly decreased cecal butyrate concentrations compared with the CON group (p &lt; 0.05), while inulin supplementation reversed these changes compared with the SE group (p &lt; 0.05). Inulin supplementation at 1% significantly increased the abundances of Lactobacillus and Streptococcus, and significantly decreased the abundances of Subdoligranulum and Sellimonas compared with the SE group (p &lt; 0.05). The functional profiles of microbial communities based on metagenomic sequencing analysis showed that SE infection significantly increased the abundances of pathways related to carbohydrate metabolism, amino acid metabolism, energy metabolism, metabolism of cofactors and vitamins, and glycan biosynthesis and metabolism (p &lt; 0.05), and significantly decreased the abundances of pathways related to nucleotide metabolism, translation, and replication and repair compared with the CON group (p &lt; 0.05), and these effects were reversed by inulin supplementation (0.5 and 1%) (p &lt; 0.05). In conclusion, inulin modulated the dysbiosis induced by SE infection via affecting SCFA metabolism and microbial functional profiles.

scholarly journals Growth performance and antioxidant status of growing rabbits fed on diets supplemented with Eucommia ulmoides leaves

2018 ◽  
Vol 26 (1) ◽  
pp. 35 ◽  
Author(s):  
Song Li ◽  
Miaoqing Zhao ◽  
Tingting Jiang ◽  
Wenwen Lv ◽  
Shujuan Gao ◽  
...  
Keyword(s):  
Growth Performance ◽  
Feed Intake ◽  
Antioxidant Status ◽  
Basal Diet ◽  
Experimental Period ◽  
Control Group ◽  
Eucommia Ulmoides ◽  
Feed Conversion ◽  
Diet Control ◽  
And Control

<p>The present study evaluated the effect of dietary <em>Eucommia ulmoides leaves</em> (EUL) on growth performance and antioxidant status of growing rabbits under heat stress condition. Four hundred and fifty weaned New Zealand male rabbits (6 wk old) were randomly divided into 3 equal groups (150 rabbits/group) and fed with a basal diet (control, digestible energy (DE): 15.92 MJ/kg and crude protein (CP): 19.24%) or the basal diet supplemented with 1 or 5 g of EUL/kg of diet (EUL1 and EUL5), in which the same quantity of barley meal was replaced. During the 21 d of experiment (43 to 63 d of age), the temperature and relative humidity of the rabbit house ranged from 27.5 to 32.5°C and from 65 to 73%, respectively. We analysed feed intake, growth performance and antioxidant status of growing rabbits. Compared with the control group, at the end of the experimental period, EUL supplementation significantly reduced the average daily feed intake (92.0, 92.8 and 100.7 g/d for EUL1, EUL5 and control, respectively; P&lt;0.05), improved the feed conversion ratio (3.80, 3.81 and 4.59 for EUL1, EUL5 and control, respectively; P&lt;0.05), increased the activities of glutathione peroxidase (+35.5 and +35.0% in plasma and liver of rabbits in EUL5 vs. control group, respectively; P&lt;0.05) and reduced those of malondialdehyde (–12.0 and –46.0% in plasma and liver of rabbits in EUL5 vs. control group, respectively; P&lt;0.05). These results suggest that inclusion of EUL in the diet of growing rabbits improved the growth performance and antioxidant status in growing rabbits.</p>

Study The Effect of Dietary Supplementation of Crushed Seeds of Coriander (Coriandrum Sativum) on Some Physiological Parameters of Rabbits.

2018 ◽  
Vol 9 (SPL1) ◽  
Author(s):  
Sura Safi Obayes Khafaji
Keyword(s):  
Total Protein ◽  
Blood Cells ◽  
White Blood Cells ◽  
Hemoglobin Concentration ◽  
Basal Diet ◽  
Experimental Period ◽  
Physiological Parameters ◽  
Coriandrum Sativum ◽  
Control Group ◽  
T1 And T2

The aim of present research to study the effects of crushed seeds of Coriander (Coriandrum sativum) on some physiological parameters in rabbits. Thirty mature male rabbits, (aged 87-100 days),were divided randomly into three equal groups. First group (control) fed the basal diet along the experimental period,2nd and 3rd groups (T1 and T2) fed the basal diet mixed with 3% and 6%,respectively. At the end of experiment,blood samples were collected from ear vein for counting the red blood cells (RBCs) and white blood cells (WBCs),estimating Hemoglobin concentration (Hb) and packed cell volume (PVC). Total protein,albumin,globulin,blood glucose,GOT and GPT concentrations were estimated in blood serum. The results showed significant increase (P<0.05) of RBCs,WBCs,Hb and PCV levels in T1 and T2 groups when compared with control group. As well as,The results of total protein and globulin increased significantly in two treated groups in comparison with control groups. While,the results of glucose,GOT and GPT levels showed a significant decrement (P<0.05) in T1 and T2 groups compared with control group. Therefore,it was concluded that the use of Coriander seeds powder can affected positively on some physiological traits in rabbits.

scholarly journals Correlation of the rumen fluid microbiome and the average daily gain with a dietary supplementation of Allium mongolicum Regel extracts in sheep1

2019 ◽  
Vol 97 (7) ◽  
pp. 2865-2877 ◽  
Author(s):  
Hongxi Du ◽  
Khas Erdene ◽  
Shengyang Chen ◽  
Saruli Qi ◽  
Zhibi Bao ◽  
...  
Keyword(s):  
Rumen Fluid ◽  
Average Daily Gain ◽  
Basal Diet ◽  
Experimental Period ◽  
Control Group ◽  
Illumina Hiseq ◽  
Rumen Microbiome ◽  
Microbiome Profile ◽  
Dietary Treatments ◽  
Powder Extract

Abstract Plant extracts can affect the rumen microbiome and ADG in ruminants, and studies of the association between the rumen microbiome and ADG provide information applicable to improving ruminant growth performance. The objectives were to investigate the effects of Allium mongolicum Regel extracts on the rumen microbiome and ADG and their association in sheep. Forty healthy, male, small-tailed Han sheep (6 mo, 34 ± 3.5 kg body weight) were randomly assigned to 1 of the following 4 dietary treatments: basal diet as control group (CK, n = 10), basal diet supplemented with 3.4 g·sheep−1·d−1A. mongolicum Regel powder extract as PAM group (PAM, n = 10), basal diet supplemented with 10 g·sheep−1·d−1A. mongolicum Regel powder as AM group (AM, n = 10), and basal diet supplemented with 10 g·sheep−1·d−1A. mongolicum Regel powder extract residue as RAM group (RAM, n = 10). The ADG for individual sheep was calculated using the sum of the ADGs observed during the experimental period divided by the number of days in the experimental period. At the end of the experiment, sheep were randomly selected from each treatment for slaughter (n = 6), and the rumen fluids were collected and stored immediately at −80 °C. Illumina HiSeq was subsequently used to investigate the changes in the rumen microbiome profile, and the associations with ADG were analyzed by Spearman correlation coefficient analysis. The results demonstrated that, compared with that in CK group, the ADG in AM and RAM significantly increased (P = 0.0171). The abundances of Tenericutes and Mollicutes ([ρ] = 0.5021, P = 0.0124) were positively correlated with ADG. Within Mollicutes, the abundances of Anaeroplasmatales ([ρ] = 0.5458, P = 0.0058) and Anaeroplasmataceae ([ρ] = 0.5458, P = 0.0058) were positively correlated with ADG. The main negatively correlated bacteria were Saccharibacteria ([ρ] = −0.4762, P = 0.0187) and Betaproteobacteria ([ρ] = −0.5669, P = 0.039). Although Anaeroplasmatales and Anaeroplasmataceae were positively correlated with ADG, Saccharibacteria and Betaproteobacteria were negatively correlated with ADG. In conclusion, supplementation with A. mongolicum Regel powder and extracts will influence the rumen microbiome and increase the ADG.

scholarly journals Effects of High-Forage Diets Containing Raw Flaxseeds or Soybean on In Vitro Ruminal Fermentation, Gas Emission, and Microbial Profile

2021 ◽  
Vol 9 (11) ◽  
pp. 2304
Author(s):  
Xiao-Ge Sun ◽  
Yue Wang ◽  
Tian Xie ◽  
Zhan-Tao Yang ◽  
Ji-Dong Wang ◽  
...  
Keyword(s):  
Soybean Seed ◽  
Basal Diet ◽  
The Other ◽  
Control Group ◽  
Ruminal Fermentation ◽  
Gas Emission ◽  
Microbial Profile ◽  
High Forage ◽  
Forage Diets

Lipid metabolism plays an important role in the energy economy of ruminants. However, its interactions of fat, rumen fermentation, gas emission, and microorganisms are not yet clear. This study evaluated the effect of adding raw oilseeds to high-forage diets on in vitro ruminal fermentation, gas composition, and microbial profile. Three isoenergetic and isoproteic experimental diets were designed and used as fermentation substrate: control treatment (CON group) was the basal diet lacking oilseeds, the other two treatments were the basal diet supplemented by 100 g/kg dry matter (DM) raw whole soybean (S group) and 50 g/kg DM raw flaxseed (F group), respectively. Data showed that the acetate, butyrate, and total VFA concentration of culture fluids in the S group were lower (p < 0.05) than in the F group. There was a tendency to a higher level (p = 0.094) of propionate concentration in the F group compared with the other two groups. The gas production in the F group was higher (p < 0.05) than in the control group. There was a lower abundance of Sutterella (p < 0.05) and a greater abundance of Butyrivibrio (p < 0.05) in both of the two oilseed treatments. Methanobrevibacter (p = 0.078) in the F group was the lowest. Our results suggested that CH4 emission could be inhibited with flaxseed supplementation by propionate production metabolism, biohydrogenation of unsaturated fatty acid (FA), and toxicity to Methanobrevibacter, while regarding soybean seed supplementation, the emission of CH4 was more likely to be reduced through biohydrogenation of unsaturated FA modulated by Butyrivibrio.

scholarly journals Dietary Supplementation Effects of Noni (Morinda Citrifolia L.) Fruit Flour on Uric Acid and Blood Glucose of Quails (Coturnix Coturnix Japonica) Layer Phase

2017 ◽  
Vol 2 (6) ◽  
pp. 80
Author(s):  
Lovita Adriani ◽  
Bigtha Yeane
Keyword(s):  
Uric Acid ◽  
Blood Glucose ◽  
Animal Husbandry ◽  
Basal Diet ◽  
Experimental Period ◽  
Morinda Citrifolia ◽  
Control Group ◽  
Treatment Groups ◽  
Randomized Design ◽  
Blood Uric Acid

This study was aimed to determine the effect of noni fruit flour in ration on  uric acid and blood glucose of  quails layer  phase. The research was conducted from  March to May 2015 at  Faculty of Animal Husbandry Universitas Padjadjaran. One hundred commercialquails aged 4 weeks were randomly allocated to four treatment groups as T1, T2, T3 and T4. The quails  in the control group (T1) were given normal basal diet without the addition of noni fruit flour, while as other groups (T2, T3, T4) were supplemented with0.25, 0.50, and 0.75% noni fruit flour  respectively with 25 quails per treatment group replicated five times with five quails per replicate in a Complete Randomized Design (CRD). The blood samples were randomly collected, five birds per replicate at the end experimental period (7th week) and analyzed for the estimation of uric acid and blood glucose. The results revealed that blood glucose was significantly (p>0.05) increased in the groups fed noni at various levels when compared to the control. Further, a significantly (p<0.05) proportional decreasing  in blood uric acid levels was found with increase in the level of dietary  noni fruit flour, with highest reduction in the group supplemented with 0.5% noni fruit flour (T3) compared to the control group and in the group suplemented with 0.25% (T2) for blood glucose. In conclusion, dietary inclusion of noni fruit flours had beneficial effect with regard to its ability in reducing the blood uric acid levels and increasing blood glucose of quails. Keywords: Quail, uric acid, blood glucose, Noni

scholarly journals Mineral composition of the organism tissues and honeycombs of melliferous bees under the conditions of feeding them soybean flour and citrates of Cobalt and Nickel

2019 ◽  
Vol 21 (93) ◽  
pp. 60-64
Author(s):  
A. G. Pashchenko ◽  
I. I. Kovalchuk ◽  
R. S. Fedoruk
Keyword(s):  
Inductively Coupled Plasma ◽  
Mineral Metabolism ◽  
Atomic Emission ◽  
Essential Elements ◽  
The Body ◽  
Soy Flour ◽  
Emission Spectrometry ◽  
Control Group ◽  
Soybean Flour ◽  
Cobalt And Nickel

The inadequacy of mineral nutrition leads to inhibition of physiological and metabolic reactions in the body of honeybees. It is known that Cobalt chloride is used to activate oviposition of the queen bee. It was established that Cobalt and Nickel citrate, obtained by the method of nanotechnology, corrects the mineral metabolism and affects the metabolism of bees. It is known that Cobalt plays an important role in the work of enzymes; synthesis of vitamin B12, promotes assimilation of vitamins A, E, C; increases protein metabolism, participates in hematopoiesis. Nickel also has a pronounced effect on hemopoiesis, namely on the morphological composition of blood. But its effect on the cell and subcellular level is not well understood. The results of studies of the effect of soybean flour with the addition of Cobalt and Nickel citrates on the content of mineral elements in the tissues of the body of bees and honeycombs are given. The research was carried out in the farms in the Lviv region, in April-May at the bees of the Carpathian breed. It was established that the content of Ferrum, Cuprum and Germanium in the tissues of bees increases with the addition of soybean flour with Сobalt citrate at a dose of 2 mg per 500 g of soy flour. When Nickel citrate was added to the feed at a dose of 1 mg per 500 g of soy flour, the content of Ferrum and Cuprum increased in honeycombs. The complex combination of Citrits Co and Ni, soy flour with sugar syrup was characterized by a decrease in the level of Zn in the tissues of the bees compared to its contents in the control group samples. In samples of biological material, the content of Сobalt, Nickel and the essential elements Cuprum, Zink, Ferrum, Selenium, Germanium was determined by atomic emission spectrometry with inductively coupled plasma. Citrates of the microelements were produced by the method of M. Kosinov and V. Kaplunenkо.

scholarly journals Effects of Increasing Levels of Defatted Rice Bran on Intestinal Physical Barrier and Bacteria in Finishing Pigs

Animals ◽  
2019 ◽  
Vol 9 (12) ◽  
pp. 1039
Author(s):  
Huan Wang ◽  
Pinghua Li ◽  
Taoran Du ◽  
Guang Pu ◽  
Lijuan Fan ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Control Diet ◽  
Lactate Concentration ◽  
Basal Diet ◽  
Experimental Period ◽  
Intestinal Wall ◽  
Control Group ◽  
Finishing Pigs ◽  
Physical Barrier ◽  
Total Bacteria

The aims of this study were to assess the effects of increasing levels of DFRB as a replacement for corns on intestinal physical barrier function and bacteria of finishing pigs. A total of 35 castrated finishing pigs (age: 158.5 ± 2.0 d, initial body weight: 62.9 ± 0.8 kg) were randomly divided into five dietary treatments (seven replicates/treatment) for a 28-day experimental period, i.e., a control diet with basal diet, and four experimental diets in which maize was replaced by 7%, 14%, 21%, and 28% DFRB, respectively. The results showed that serum endotoxins concentration and diamine oxidase (DAO) activity were both increased (linear, p = 0.0004, 0.001, respectively) with DFRB level. However, compared with control group, serum endotoxins concentration and DAO activity were not different in pigs fed with 7% DFRB in the diet. There was a quadratic response in serum D-lactate concentration to the increased DFRB (quadratic, p = 0.021). In the cecum, thickness of the intestinal wall significantly increased with increasing levels of DFRB in the diets (linear, p = 0.033), while crypt depth/thickness of the intestinal wall ratio significantly decreased with increasing level of DFRB in the diets (linear, p = 0.043). In the jejunum, total bacteria, Escherichia coli, and Bifidobacterium all responded quadratically to increasing levels of DFRB in the diets (quadratic, p = 0.003, 0.001, 0.006, respectively). Additionally, there was no difference in Escherichia coli in pigs fed 0%, 7%, and 14% DFRB diets. In the colon, there were quadratic responses in C. perfringens to the increased DFRB (quadratic, p = 0.023). C. perfringens reduced as the DFRB concentration increased from 0% to 14% and then increased. When D-lactate, total bacteria, Escherichia coli, Bifidobacterium, and C. perfringens were considered, the optimal substitution level of DFRB were 12.00%, 11.84%, 7.50%, 8.92%, and 15.92%, respectively. In conclusion, 7% DFRB had a beneficial effect on intestinal wall thickness, Bifidobacterium and C. perfringens, and had no adverse effect on intestinal permeability and Escherichia coli.

Sign in / Sign up

Export Citation Format

Share Document