PSV-29 Changes in the PSE and DFD porcine muscle proteome during autolysis

Abstract Despite reducing of pale, soft, exudative (PSE) pork share, its amount is 45% in Russia and 65% in Belarus of the total. Dark, firm, dry (DFD) pork occurrence increases to 20% in Russia and 30% in Belarus. Protein changes in normal (NOR), PSE and (DFD) pork muscle during 5 days ageing were studied. Were found differences in their protein profiles and potential protein markers of such raw materials were identified. Mainly in range 600–1000 Da and up to 450 Da. Analysis of the peptide composition showed two myosin light chain isoforms (MLC) (Mw/pI): MLC-like protein — 21.8/5.00 and phosphorylated MLC f -21.0/4.90, in the sample. One of which is fast, the other is slow MLC isoform. Increase in the number of fragments was noted of such proteins as pyruvatekinase, α-enolase, muscle creatine phosphokinase and GPDH, adenylatekinase, αB-crystallin. A correlation between muscle proteins breakdown (the troponin group/troponin family) and the characteristics of meat was found. In NOR samples, troponin I decomposed quite clearly. The most obvious autolysis marker is the appearance and increase of three additional fragments of fast skeletal muscle troponin T, which differ in molecular weight and pI. According to the tryptic peptides mass spectra, the C- and N-molecule terminal parts peptides are no longer detected sequentially. Moreover, in DFD samples, they are most significantly split into shorter peptides. Appearance of short N-terminal GPDH 21 kDa fragment on the 5th day of autolysis is PSE specific marker. Totally 12, 14 and 18 specific peptides were identified in chilled and 18, 32 and 38 on 5th day of autolysis in PSE, NOR and DFD pork respectively. DFD meat can be considered a source of natural specific low-molecular peptides, specifically to produce meat products with functional properties. (Research was supported by the Russian Science Foundation, project 16-16-10073)

Download Full-text

Sensitivity and specificity of cardiac troponin-T in diagnosis of acute myocardial infarction

International Journal of Advances in Medicine ◽

10.18203/2349-3933.ijam20170119 ◽

2017 ◽

Vol 4 (1) ◽

pp. 244 ◽

Cited By ~ 1

Author(s):

Dharmveer Sharma ◽

Poonam Gupta ◽

Sagar Srivastava ◽

Harshit Jain

Keyword(s):

Myocardial Infarction ◽

Acute Myocardial Infarction ◽

Cardiac Troponin ◽

Troponin I ◽

Striated Muscle ◽

Troponin T ◽

T Test ◽

Specific Marker ◽

Enzymatic Markers ◽

Better Than

Background: Myocardial Infarction is the irreversible necrosis of the heart muscle secondary to prolong lack of oxygen supply. Troponin T is a structurally bound protein found in striated muscle cells. They have rapidly attained central role in diagnosis, prognostication and planning of therapeutic strategies in MI patients. The objective of this study was to evaluate the status of Troponin T in MI patients and its role in diagnosis compare to normal subjects.Methods: The study was conducted at M. L .N. Medical College, Allahabad, Uttar Pradesh India. A total of 136 cases were included in our study. Out of these, 86 were patients of AMI and 50 were healthy controls. They were evaluated by measurement of various parameters including enzymatic markers such as CPK-MB, SGOT, LDH1, and LDH2 and non-enzymatic markers such as troponin-T and myoglobin. Apart from these, LDL, VLDL and HDL levels were also kept under evaluation.Results: Troponin-T test was better than CPK-MB or SGOT in diagnosing myocardial infarction. In our study, sensitivity (67.3%) and specificity (73.8%) of troponin-T test was higher than CPK-MB (56.2% and 45.7%) and SGOT (34.2% and 58.3%) respectively. Troponin-T test was better than CPK-MB or SGOT after 2 hours of onset of myocardial infarction. Troponin- T and I: both kind of evaluations are available and are well evaluated. However troponin T estimation is more standardized and thus more popular. The positivity of troponin-T test also varied with area of infarct.Conclusions: High LDL and VLDL were seen while at the same time HDL level was lowered. An Increase in the level of myoglobin (non-specific marker), Cardiac troponin I and T and among the enzymatic markers elevated levels of CPK-MB, LDH and SGOT were observed in patients of MI against the normal subjects. In case of LDH both LDH 1 and LDH 2 were observed and a flipped pattern was noted. Bedside troponin-T test is highly sensitive and specific in the diagnosis of acute myocardial infarction and can be used in emergency and ambulatory settings. Qualitative troponin-T test is reliable above serum level of ≥ 0.10 ng/ml.

Download Full-text

MEMBRANE TESTS FOR IDENTIFICATION OF RAW MATERIALS IN MEAT PRODUCTS

10.47501/978-5-6044060-1-4.32 ◽

2021 ◽

Author(s):

Elena Anatolievna Zvereva ◽

◽

Olga Dmitrievna Hendrickson ◽

Demid Sergeevich Popravko ◽

Anatoly Vitalievich Zherdev ◽

...

Keyword(s):

Troponin I ◽

Raw Materials ◽

Meat Products ◽

Protein Biomarkers ◽

Raw Meat ◽

Test Systems

Membrane test systems have been developed for rapid out-of-laboratory identification of raw meat using immunochromatographic detection of protein biomarkers – troponin I, myoglobin, immunoglobulin. Their effectiveness has been shown for the control of various types of meat-containing products.

Download Full-text

Toward Development of a Label-Free Detection Technique for Microfluidic Fluorometric Peptide-Based Biosensor Systems

Micromachines ◽

10.3390/mi12060691 ◽

2021 ◽

Vol 12 (6) ◽

pp. 691

Author(s):

Nikita Sitkov ◽

Tatiana Zimina ◽

Alexander Kolobov ◽

Vladimir Karasev ◽

Alexander Romanov ◽

...

Keyword(s):

Troponin I ◽

Troponin T ◽

Detection System ◽

Label Free ◽

Protein Markers ◽

Peptide Aptamers ◽

Registration System ◽

Biosensor System ◽

Label Free Detection ◽

Selection Of

The problems of chronic or noncommunicable diseases (NCD) that now kill around 40 million people each year require multiparametric combinatorial diagnostics for the selection of effective treatment tactics. This could be implemented using the biosensor principle based on peptide aptamers for spatial recognition of corresponding protein markers of diseases in biological fluids. In this paper, a low-cost label-free principle of biomarker detection using a biosensor system based on fluorometric registration of the target proteins bound to peptide aptamers was investigated. The main detection principle considered includes the re-emission of the natural fluorescence of selectively bound protein markers into a longer-wavelength radiation easily detectable by common charge-coupled devices (CCD) using a specific luminophore. Implementation of this type of detection system demands the reduction of all types of stray light and background fluorescence of construction materials and aptamers. The latter was achieved by careful selection of materials and design of peptide aptamers with substituted aromatic amino acid residues and considering troponin T, troponin I, and bovine serum albumin as an example. The peptide aptamers for troponin T were designed in silico using the «Protein 3D» (SPB ETU, St. Petersburg, Russia) software. The luminophore was selected from the line of ZnS-based solid-state compounds. The test microfluidic system was arranged as a flow through a massive of four working chambers for immobilization of peptide aptamers, coupled with the optical detection system, based on thick film technology. The planar optical setup of the biosensor registration system was arranged as an excitation-emission cascade including 280 nm ultraviolet (UV) light-emitting diode (LED), polypropylene (PP) UV transparent film, proteins layer, glass filter, luminophore layer, and CCD sensor. A laboratory sample has been created.

Download Full-text

Problems of ensuring metrological traceability of measurement results of indicators of quality for food products and food raw materials

Izmeritel`naya Tekhnika ◽

10.32446/0368-1025it.2020-3-64-70 ◽

2020 ◽

pp. 64-70

Author(s):

Mariya Y. Medvedevskikh ◽

Anna S. Sergeeva

Keyword(s):

Reference Materials ◽

Mass Fraction ◽

Nutritional Value ◽

Animal Feed ◽

Raw Materials ◽

Meat Products ◽

Metrological Traceability ◽

Food Products ◽

Freeze Dried ◽

Measurement Results

The article raises the problem of ensuring metrological traceability of the measurement results of indicators of quality and nutritional value for food products and food raw materials: water (moisture), nitrogen (protein, crude protein), fat, ash and carbohydrates. The problem under consideration can be solved by applying reference materials of food composition, traceable to state primary measurement standards GET 173-2017 and GET 176-2019 and primary reference measurement procedures (PRMP), for attestation of measurement procedures and accuracy checking of measurement results. The article discusses the results of the PRMP development of mass fraction of fat, ash and carbohydrates in food products and food raw materials, as well as mass fraction of crude fat (oil content) in oil crops seeds and products based on them. The paper also presents metrological characteristics of reference materials of composition of dry dairy products, grain-milk dry porridges for nutrition of babies, grain dry porridges for nutrition of babies, egg powder, freeze-dried meat products, animal feed. The results of the work allow for building a chain of metrological traceability from GET 173-2017, GET 176-2019 and PRMP to routine measurement procedures, thereby ensuring the uniformity of measurements of nutritional value of food products.

Download Full-text

1461-P: Cardiac Troponin T and Troponin I and Incident Diabetes in the General Population: Generation Scotland Scottish Family Health Study

Diabetes ◽

10.2337/db19-1461-p ◽

2019 ◽

Vol 68 (Supplement 1) ◽

pp. 1461-P

Author(s):

PAUL WELSH ◽

DAVID PREISS ◽

ARCHIE CAMPBELL ◽

DAVID J. PORTEOUS ◽

NICHOLAS L. MILLS ◽

...

Keyword(s):

General Population ◽

Cardiac Troponin ◽

Troponin I ◽

Troponin T ◽

Family Health ◽

Incident Diabetes ◽

Health Study ◽

Cardiac Troponin T ◽

Generation Scotland

Download Full-text

Ukraine’s foreign trade in meat and meat products: trends and geographical aspects

Socio-Economic Problems of the Modern Period of Ukraine ◽

10.36818/2071-4653-2020-3-7 ◽

2020 ◽

pp. 48-52

Author(s):

Olena Lyakhovska

Keyword(s):

Small Businesses ◽

Structural Changes ◽

Raw Materials ◽

Meat Products ◽

World Market ◽

Poultry Meat ◽

Positive Development ◽

Meat Production ◽

Meat Industry ◽

Geographical Structure

Current trends in meat and meat products exports are considered. The dynamics and structural changes in meat exports by types and degree of processing (beef and veal, pork, poultry, finished meat products) are analyzed. The paper proves that in recent years, only meat and edible poultry by-products have taken a competitive position in the world market. Accordingly, the dynamics of exports of this type of meat is positive, and the share of these products in the structure of exported meat is the largest (86.09%). Possible threats to the growth of import dependence on certain types of meat products, in particular, chilled and frozen beef and veal, pork, are indicated. The rating of the largest countries-importers of meat and meat products (by types) from Ukraine is built, their share in the structure of meat exports is determined. Changes in the geographical structure of exports have been studied, which indicate a low level of diversification of the geography of exports of certain types of meat (pork, beef). At the same time, a positive development of the geographical structure of poultry meat exports (exported to more than 100 countries) was revealed. Problems of the export potential development in the meat industry of Ukraine are revealed. In particular, the problems of livestock development (outdated technologies and production techniques that reduce the efficiency and productivity; inefficiency of the organization of purchase and processing of meat, which leads to reduced breeding in small businesses and households; inefficiency of state incentives, etc.) and industrial meat production (lack of raw materials, obsolescence of fixed assets and production technologies, poorly developed logistics, etc.). The solution of these problems requires: a systematic approach, which will encourage the cooperation of agricultural and industrial production, cooperation with importers of meat products and protection of domestic producers in the domestic and international markets, creating a favorable investment and information environment that will promote the meat industry development.

Download Full-text

Postmortem Specificity of Troponin for Acute Miocard Infarction Diagnosis throug Qualitative Dosing from Pericardial Fluid

Revista de Chimie ◽

10.37358/rc.18.9.6559 ◽

2018 ◽

Vol 69 (9) ◽

pp. 2482-2486

Author(s):

Iuliana Hunea ◽

Simona Irina Damian ◽

Carmen Corina Radu ◽

Sorin Moldoveanu ◽

Tatiana Iov

Keyword(s):

Sudden Cardiac Death ◽

Cardiac Disease ◽

Cardiac Death ◽

Troponin I ◽

Troponin T ◽

Morphological Changes ◽

Pericardial Fluid ◽

Histological Changes ◽

Lethal Arrhythmia ◽

Myocardial Lesions

Cardiac disease is the leading cause of death, and sudden cardiac death occupies the first place in sudden deaths of natural causes. Sudden cardiac death due to lethal arrhythmia may be the first manifestation of a cardiac disease, such cases becoming suspect dead, thus forensic cases. The autopsy performed in such cases may reveal important cardiovascular disease but not obvious macroscopic or histological changes of acute myocardial infarction (IMA), except for cases of survival for several hours after the onset of the symptomatology. Biochemical markers were used to test for myocardial lesions in the absence of morphological changes. Methods for determining myoglobin, CK-MB, troponin T (cTn T), troponin I (cTn I) were introduced to the clinic to diagnose the condition of patients with chest pain as early as the 1990s. The lack of pathognomonic elements in corps investigations, where part of the analysis cannot be carried out, requires verification of the value of the investigations that can be carried out, with reference to the biochemical in the present case, in establishing the diagnosis with certainty.

Download Full-text

Оrganic Meat Production of Broiler Chickens Hubbard Redbro Cross

International Journal of Veterinary Science ◽

10.37422/ijvs/20.072 ◽

2020 ◽

Keyword(s):

Broiler Chickens ◽

Raw Materials ◽

Animal Health ◽

Meat Products ◽

Poultry Meat ◽

Meat Production ◽

Significant Difference ◽

Environmentally Safe ◽

The Impact

This article presents the results of studying the impact of housing and feeding conditions on broiler chickens of Hubbard RedBro cross, as well as the quality of products obtained when using floor and cage content, in a farm. It established that when receiving a mixed feed of own production using feed raw materials grown on a farm without the use of pesticides, a statistically significant decrease in potentially dangerous substances for animal health is recorded. Compared with factory feed, it has reduced the content of pesticides by 14 times, and mercury and arsenic by 24 times, cadmium by five times, and lead by ten times. The results of the study of economic indicators of growing Hubbard RedBro cross broiler chickens, as well as the chemical composition and quality of carcasses, indicated that there was no significant difference between the floor and cell conditions of keeping. Still, the use of a diet based on eco-feeds contributed to a statistically significant decrease in the concentration of toxic metals in the muscles of the poultry of the experimental groups. As a result, it found that the use of the studied compound feed in the diets of broiler chickens increased the indicators of Biosafety and ensured the production of environmentally safe ("organic") poultry meat products.

Download Full-text

Development of Immunohistochemical Methods for Casein Detection in Meat Products

Foods ◽

10.3390/foods10010028 ◽

2020 ◽

Vol 10 (1) ◽

pp. 28

Author(s):

Ludmila Kalčáková ◽

Matej Pospiech ◽

Bohuslava Tremlová ◽

Zdeňka Javůrková ◽

Irina Chernukha

Keyword(s):

Milk Protein ◽

Production Efficiency ◽

Raw Materials ◽

Meat Products ◽

Milk Proteins ◽

Increase Production Efficiency ◽

Allergenic Potential ◽

Significant Difference ◽

Dairy Ingredients ◽

Immunohistochemical Methods

To increase production efficiency of meat products, milk protein additives are often used. Despite a number of advantages, use of dairy ingredients involves a certain risk, namely the allergenic potential of milk proteins. A number of methods have been developed to detect milk-origin raw materials in foodstuffs, including immunological reference methods. This study presents newly developed immunohistochemical (IHC) methods for casein detection in meat products. Casein was successfully detected directly in meat products where sensitivity was determined at 1.21 and specificity at 0.28. The results obtained from the IHC were compared with the Enzyme-Linked Immuno Sorbent Assay (ELISA) and there was no statistically significant difference between the IHC and ELISA methods (p > 0.05). The correspondence between the methods was 72% in total. The highest correspondence was reached in frankfurters (90%), the lowest in canned pâté (44%).

Download Full-text