Abscisic acid homeostasis is mediated by feedback regulation of MdMYB88 and MdMYB124

2020 ◽  
Author(s):  
Yinpeng Xie ◽  
Chana Bao ◽  
Pengxiang Chen ◽  
Fuguo Cao ◽  
Xiaofang Liu ◽  
...  
Keyword(s):  
Abscisic Acid ◽  
Woody Plants ◽  
Regulatory Mechanism ◽  
Photosynthetic Capacity ◽  
Feedback Regulation ◽  
Biosynthetic Gene ◽  
Promoter Regions ◽  
Apple Trees ◽  
Catabolic Genes ◽  
Water Transpiration

Abstract The phytohormone abscisic acid (ABA) is involved in various plant processes. In response to drought stress, plants quickly accumulate ABA, but the regulatory mechanism of ABA accumulation is largely unknown, especially in woody plants. In this study, we report that MdMYB88 and MdMYB124 are myeloblastosis (MYB) transcription factors critical for ABA accumulation in apple trees (Malus x domestica) following drought, and this regulation is negatively controlled by ABA. MdMYB88 and MdMYB124 positively regulate leaf water transpiration, photosynthetic capacity, and stress endurance in apple trees under drought conditions. MdMYB88 and MdMYB124 regulate the expression of biosynthetic and catabolic genes of ABA, as well as drought- and ABA- responsive genes. MdMYB88 associates with promoter regions of the ABA biosynthetic gene 9-cis-epoxycarotenoid dioxygenase 3 (NCED3). Finally, expression of MdMYB88 and MdMYB124 is repressed by ABA. Our results identify a feedback regulation of MdMYB88 and MdMYB124 in modulating ABA homeostasis in apple trees.

scholarly journals Plant Hormone Metabolome and Transcriptome Analysis of Dwarf and Wild-type Banana

2021 ◽  
Author(s):  
Biao Deng ◽  
Xuan Wang ◽  
Xing Long ◽  
Ren Fang ◽  
Shuangyun Zhou ◽  
...  
Keyword(s):  
Signal Transduction ◽  
Transcriptome Analysis ◽  
Regulatory Mechanism ◽  
Feedback Regulation ◽  
Wild Type ◽  
Hormone Levels ◽  
Transporter Protein ◽  
Repressor Proteins ◽  
Differential Gene ◽  
The Relationship

AbstractGibberellin (GA), auxin (IAA) and brassinosteroid (BR) are indispensable in the process of plant growth and development. Currently, research on the regulatory mechanism of phytohormones in banana dwarfism is mainly focused on GA, and few studies are focused on IAA and BR. In this study, we measured the contents of endogenous GA, IAA and BR and compared the transcriptomes of wild-type Williams banana and its dwarf mutant across five successive growth periods. We investigated the relationship between hormones and banana dwarfism and explored differential gene expression through transcriptome analysis, thus revealing the possible metabolic regulatory mechanism. We inferred a complex regulatory network of banana dwarfing. In terms of endogenous hormone levels, GA and IAA had significant effects on banana dwarfing, while BR had little effect. The key gene in GA biosynthesis of is GA2ox, and the key genes in IAA biosynthesis are TDC and YUCCA. The differential expression of these genes might be the main factor affecting hormone levels and plant height. In terms of hormone signal transduction, DELLA and AUX/IAA repressor proteins were the core regulators of GA and IAA, respectively. They inhibited the process of signal transduction and had feedback regulation on hormone levels. Finally, the transporter protein PIN, AUX1/LAX protein family and ABCB subfamily played supplementary roles in the transport of IAA. These results provide new insights into GA and IAA regulation of banana growth and a reliable foundation for the improvement of dwarf varieties.

scholarly journals Foxd4l1.1 negatively regulates transcription of neural repressor ventx1.1 during neuroectoderm formation in Xenopus embryos

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Shiv Kumar ◽  
Zobia Umair ◽  
Vijay Kumar ◽  
Santosh Kumar ◽  
Unjoo Lee ◽  
...  
Keyword(s):  
Regulatory Mechanism ◽  
Bmp Signaling ◽  
Promoter Regions ◽  
Occupied Territory ◽  
Neural Genes ◽  
Synergistic Activation ◽  
Direct Binding ◽  
Neural Ectoderm ◽  
Developmental Decision ◽  
Primary Neurogenesis

Abstract Neuroectoderm formation is the first step in development of a proper nervous system for vertebrates. The developmental decision to form a non-neural ectoderm versus a neural one involves the regulation of BMP signaling, first reported many decades ago. However, the precise regulatory mechanism by which this is accomplished has not been fully elucidated, particularly for transcriptional regulation of certain key transcription factors. BMP4 inhibition is a required step in eliciting neuroectoderm from ectoderm and Foxd4l1.1 is one of the earliest neural genes highly expressed in the neuroectoderm and conserved across vertebrates, including humans. In this work, we focused on how Foxd4l1.1 downregulates the neural repressive pathway. Foxd4l1.1 inhibited BMP4/Smad1 signaling and triggered neuroectoderm formation in animal cap explants of Xenopus embryos. Foxd4l1.1 directly bound within the promoter of endogenous neural repressor ventx1.1 and inhibited ventx1.1 transcription. Foxd4l1.1 also physically interacted with Xbra in the nucleus and inhibited Xbra-induced ventx1.1 transcription. In addition, Foxd4l1.1 also reduced nuclear localization of Smad1 to inhibit Smad1-mediated ventx1.1 transcription. Foxd4l1.1 reduced the direct binding of Xbra and Smad1 on ventx1.1 promoter regions to block Xbra/Smad1-induced synergistic activation of ventx1.1 transcription. Collectively, Foxd4l1.1 negatively regulates transcription of a neural repressor ventx1.1 by multiple mechanisms in its exclusively occupied territory of neuroectoderm, and thus leading to primary neurogenesis. In conjunction with the results of our previous findings that ventx1.1 directly represses foxd4l1.1, the reciprocal repression of ventx1.1 and foxd4l1.1 is significant in at least in part specifying the mechanism for the non-neural versus neural ectoderm fate determination in Xenopus embryos.

scholarly journals Impaired sucrose-induction mutants reveal the modulation of sugar-induced starch biosynthetic gene expression by abscisic acid signalling

2001 ◽  
Vol 26 (4) ◽  
pp. 421-433 ◽  
Author(s):  
Fred Rook ◽  
Fiona Corke ◽  
Roderick Card ◽  
Georg Munz ◽  
Caroline Smith ◽  
...  
Keyword(s):  
Gene Expression ◽  
Abscisic Acid ◽  
Biosynthetic Gene ◽  
Sucrose Induction ◽  
Biosynthetic Gene Expression

scholarly journals Drought Response of Young Apple Trees on Three Rootstocks. II. Gas Exchange, Chlorophyll Fluorescence, Water Relations, and Leaf Abscisic Acid

1997 ◽  
Vol 122 (6) ◽  
pp. 841-848 ◽  
Author(s):  
R. Thomas Fernandez ◽  
Ronald L. Perry ◽  
James A. Flore
Keyword(s):  
Abscisic Acid ◽  
Chlorophyll Fluorescence ◽  
Drought Stress ◽  
Gas Exchange ◽  
Water Relations ◽  
Acid Content ◽  
Recovery Period ◽  
Leaf Conductance ◽  
Apple Trees ◽  
Abscisic Acid Content

`Imperial Gala' apple trees (Malus ×domestica Borkh.) on M.9 EMLA, MM.111, and Mark rootstocks were subjected to two drought-stress and recovery periods in a rainshelter. Water relations, gas-exchange parameters per unit leaf area and per tree, chlorophyll fluorescence, and leaf abscisic acid content were determined during each stress and recovery period. Whole-plant calculated gas exchange best indicated plant response to drought stress, with consistent reductions in CO2 assimilation, transpiration, and leaf conductance. Variable and maximal chlorophyll fluorescence and fluorescence quenching were not as sensitive to stress. Other fluorescence parameters showed little difference. The most consistent decreases due to stress for gas exchange per square meter were in transpiration and leaf conductance, with few differences in CO2 assimilation and fewer for mesophyll conductance, internal CO2 concentration, and water-use efficiency. Leaf water potential was consistently lower during drought stress and returned to control values upon irrigation. Leaf abscisic acid content was higher for drought-stressed trees on M.9 EMLA than control trees during the stress periods but inconsistently different for the other rootstock treatments. Trees on M.9 EMLA were least affected by drought stress, MM.111 was intermediate, and Mark was the most sensitive; these results are consistent with the growth data.

scholarly journals Effect of exogenously application selected phytohormonal substances on the physiological and morphological indicators of Philadelphus x hybrid in containers

2012 ◽  
Vol 60 (8) ◽  
pp. 109-118
Author(s):  
Martin Jonáš ◽  
P. Salaš ◽  
T. Baltazár
Keyword(s):  
Abscisic Acid ◽  
Chlorophyll Fluorescence ◽  
Stomatal Conductance ◽  
Chlorophyll Content ◽  
Significant Influence ◽  
Woody Plants ◽  
Stress Factors ◽  
Morphological Parameters ◽  
Physiological Indicators ◽  
The Impact

The experiment was established in order to eliminate the effect of stress factors acting on woody plants cultivated in containers. The timber Philadelphus x hybrid ’Mont Blanc’. The timber was in 1.5 l containers. The experiment was evaluated effect of exogenouse application of phytohormonal substances on select physiological indicators (stomatal conductance, chlorophyll fluorescence and chlorophyll content), were measured three times during the vegetation. Morphological indicators (diameter of root neck, total length of shoots and number of shoots), were evaluated at the end of vegetation. Phytohormones affecting the impact of stress on plants were used for the purpose. Abscisic acid, 24- epibrassinolid, kinetin and spermine were applied by spraying the leaf in three concentrations (0.01 mg.l−1, 0.1 mg.l−1 a 1 mg.l−1). In the results were found highly significant differences compared to controls with other variants, especially in the evaluation of physiological parameters. The most significant influence on the stomatal conductance was observed in the variants treated with abscisic acid. Application 24- epibrassinolid significantly increased the chlorophyll content in comparison with control variant. Morphological parameters reached the best results in variants treated with 24- epibrassinolid and spermine.

scholarly journals Effect of gravity on the distribution of growth inhibitors in apple tree

2015 ◽  
Vol 26 (1) ◽  
pp. 161-165
Author(s):  
B. Borkowska
Keyword(s):  
Abscisic Acid ◽  
Apple Tree ◽  
Horizontal Position ◽  
Growth Inhibitors ◽  
Apple Trees ◽  
Asymmetrical Distribution ◽  
Short Period

The aim of this work was to find, if there is an asymmetrical distribution of growth inhibitors in apple trees being kept in horizontal position for a short period of time. Two inhibitors were found in the extract of bark and young shoots of investigated trees. These inhibitors are identical with phloridzin and abscisic acid. The content of phloridzin was higher in the samples taken from the upper side than from the lower ones. The results showed lack of asymmetry in ABA distribution.

scholarly journals The miR528-D3 module regulates plant height in rice by modulating the gibberellin and abscisic acid metabolisms

2021 ◽  
Author(s):  
Xing Liu ◽  
Juan Zhao ◽  
Mei Wang ◽  
Lingjuan Xie ◽  
Zhengxin Wu ◽  
...  
Keyword(s):  
Abscisic Acid ◽  
Transgenic Plants ◽  
Plant Height ◽  
Target Genes ◽  
Regulatory Mechanism ◽  
Agronomic Traits ◽  
Substantial Reduction ◽  
Aba Biosynthesis ◽  
Lower Plant ◽  
Ga Content

Abstract Background Plant height, as one of the important agronomic traits of rice, is closely related to yield. In recent years, plant height-related genes have been characterized and identified, among which the D3 gene is one of the target genes of miR528, and regulates rice plant height and tillering mainly by affecting strigolactone (SL) signal transduction. However, it remains unknown whether the miR528 and D3 interaction functions in controlling plant height, and the underlying regulatory mechanism in rice. Results In this study, we found that the plant height, internode length, and cell length of internodes of d3 mutants and miR528-overexpressing (OE-miR528) lines were greatly shorter than WT, D3-overexpressing (OE-D3), and miR528 target mimicry (OE-MIM528) transgenic plants. Knockout of D3 gene (d3 mutants) or miR528-overexpressing (OE-miR528) triggers a substantial reduction of gibberellin (GA) content, but a significant increase of abscisic acid (ABA) accumulation than in WT. The d3 and OE-miR528 transgenic plants were much more sensitive to GA, but less sensitive to ABA than WT. Moreover, the expression level of GA biosynthesis-related key genes, including OsCPS1, OsCPS2, OsKO2 and OsKAO was remarkably higher in OE-D3 plants, while the NECD2 expression, a key gene involved in ABA biosynthesis, was significantly higher in d3 mutants than in WT and OE-D3 plants. Conclusion The results indicate that the miR528-D3 module negatively regulates plant height in rice by modulating the GA and ABA homeostasis, thereby further affecting the elongation of internodes, and resulting in lower plant height, which adds a new regulatory role to the D3-mediated plant height controlling in rice.

Sign in / Sign up

Export Citation Format

Share Document