scholarly journals The wheat Seven in Absentia gene is associated with increases in biomass and yield in hot climates

2021 ◽  
Author(s):  
Pauline Thomelin ◽  
Julien Bonneau ◽  
Chris Brien ◽  
Radoslaw Suchecki ◽  
Ute Baumann ◽  
...  
Keyword(s):  
High Temperatures ◽  
Positional Cloning ◽  
Plant Biomass ◽  
Wheat Chromosome ◽  
Triticum Aestivum L ◽  
Growth Conditions ◽  
Wheat Breeding ◽  
Sequence Variant ◽  
Positive Allele ◽  
Seven In Absentia

Abstract Wheat (Triticum aestivum L.) productivity is severely reduced by high temperatures. Breeding of heat tolerant cultivars can be achieved by identifying genes controlling physiological and agronomical traits when high temperatures occur and using these to select superior genotypes, but no gene underlying genetic variation for heat tolerance has previously been described. We advanced the positional cloning of qYDH.3BL, a quantitative trait locus (QTL) on bread wheat chromosome 3B associated with increased yield in hot and dry climates. The delimited genomic region contained 12 putative genes and a sequence variant in the promoter region of one gene, Seven in absentia, TaSINA. This was associated with the QTL’s effects on early vigour, root growth, plant biomass and yield components in two distinct wheat populations grown under various growth conditions. Near isogenic lines carrying the positive allele at qYDH.3BL under-expressed TaSINA and had increased vigour and water use efficiency early in development, as well as increased biomass, grain number and grain weight following heat stress. A survey of worldwide distribution indicated that the positive allele became widespread from the 1950s through the CIMMYT wheat breeding programme but, to date, has been selected only in breeding programmes in Mexico and Australia.

scholarly journals The wheat Seven in Absentia gene is associated with increases in biomass and yield in hot climates

2019 ◽  
Author(s):  
Pauline Thomelin ◽  
Julien Bonneau ◽  
Chris Brien ◽  
Radoslaw Suchecki ◽  
Ute Baumann ◽  
...  
Keyword(s):  
Genetic Variation ◽  
Heat Stress ◽  
High Temperature ◽  
Heat Tolerance ◽  
Positional Cloning ◽  
Genomic Region ◽  
Sequence Variant ◽  
Positive Allele ◽  
Breeding Programmes ◽  
Seven In Absentia

AbstractWheat productivity is severely reduced by high temperatures. Breeding of heat tolerant cultivars can be achieved by identifying genes controlling physiological and agronomical traits with high temperature and using these to select superior genotypes, but no gene underlying genetic variation for heat tolerance has previously been described. We completed the positional cloning of qYDH.3BL, a quantitative trait locus (QTL) on bread wheat chromosome 3B associated with increased yield in hot and dry climates. The delimited genomic region contained 12 putative genes and a sequence variant in the promoter region of one gene - seven in absentia, TaSINA. This was associated with the QTL’s effects on early vigour, plant biomass and yield components in two distinct wheat populations grown under various growth conditions. Near isogenic lines carrying the positive allele at qYDH.3BL under-expressed TaSINA and had increased vigour and water use efficiency early in development, as well as increased biomass, grain number and grain weight following heat stress. A survey of worldwide distribution indicated that the positive allele became widespread from the 1950s through the CIMMYT wheat breeding programme but, to date, has been selected only in breeding programmes in Mexico and Australia.Significance statementWheat is the world’s most widely grown crop and a staple of human diet. Even brief episodes of high temperature in the growing season cause severe yield reductions. Finding and deploying genes for heat stress tolerance in new varieties is a priority for food security with climate change. We narrowed a genetic locus to a small genomic region where genetic variation was present only in one gene that showed clear differences of expression and improved yield and physiology under stress in the populations. Using diagnostic markers to track the positive haplotype in nearly 750 accessions, we found many regions where the allele could be used in breeding programmes to increase wheat’s heat tolerance.

Identification of microsatellite markers associated with the cereal cyst nematode resistance gene Cre3 in wheat

2004 ◽  
Vol 55 (12) ◽  
pp. 1205 ◽  
Author(s):  
E. M. Martin ◽  
R. F. Eastwood ◽  
F. C. Ogbonnaya
Keyword(s):  
Microsatellite Markers ◽  
Microsatellite Marker ◽  
Wheat Chromosome ◽  
Triticum Aestivum L ◽  
Wheat Breeding ◽  
Cyst Nematode ◽  
Cereal Cyst Nematode ◽  
Heterodera Avenae ◽  
Pcr Marker ◽  
Diverse Range

Cereal cyst nematode (CCN) is a root disease caused by the pathogen Heterodera avenae Woll. that significantly reduces wheat (Triticum aestivum L.) grain yields in temperate countries. The Cre3 gene, located on chromosome 2DL, provides high levels of resistance to the Australian pathotype and isolates from Syria and Algeria, and has become available to wheat breeders. Selection for lines carrying the Cre3 gene in Australian wheat breeding programs is currently based on a dominant PCR marker (Cre3spf/r) diagnostic for the Cre3 gene. However, this marker has limitations that increase the cost and reduce selection efficiency in screening early-generation breeding lines. Such limitations would be minimised by the identification of a microsatellite marker linked to the Cre3 gene. We have constructed 2 genetic linkage maps of wheat chromosome 2DL and identified microsatellite markers mapping closely to the diagnostic Cre3spf/r marker. These closely linked markers were validated in a diverse range of germplasm, and one microsatellite marker, Xgwm301, which mapped 4 cM from Cre3spf/r, was shown to be highly associated with the presence of the Cre3 gene. Amplification conditions for the Xgwm301 locus were optimised, and its use in marker-assisted selection to identify Cre3 CCN-resistant wheat in the Australian Grain Technologies breeding program is demonstrated.

scholarly journals Naturally Occurring Ecdysteroids in Triticum aestivum L. and Evaluation of Fenarimol as a Potential Inhibitor of Their Biosynthesis in Plants

2021 ◽  
Vol 22 (6) ◽  
pp. 2855
Author(s):  
Anna Janeczko ◽  
Jana Oklestkova ◽  
Danuše Tarkowská ◽  
Barbara Drygaś
Keyword(s):  
Triticum Aestivum ◽  
Winter Wheat ◽  
Triticum Aestivum L ◽  
Growth Conditions ◽  
Animal Kingdom ◽  
Experimental Conditions ◽  
P450 Monooxygenase ◽  
Naturally Occurring ◽  
Regulatory Effects ◽  
Potential Use

Ecdysteroids (ECs) are steroid hormones originally found in the animal kingdom where they function as insect molting hormones. Interestingly, a relatively high number of these substances can also be formed in plant cells. Moreover, ECs have certain regulatory effects on plant physiology, but their role in plants still requires further study. One of the main aims of the present study was to verify a hypothesis that fenarimol, an inhibitor of the biosynthesis of ECs in the animal kingdom, also affects the content of endogenous ECs in plants using winter wheat Triticum aestivum L. as a model plant. The levels of endogenous ECs in winter wheat, including the estimation of their changes during a course of different temperature treatments, have been determined using a sensitive analytical method based on UHPLC-MS/MS. Under our experimental conditions, four substances of EC character were detected in the tissue of interest in amounts ranging from less than 1 to over 200 pg·g−1 FW: 20-hydroxyecdysone, polypodine B, turkesterone, and isovitexirone. Among them, turkesterone was observed to be the most abundant EC and accumulated mainly in the crowns and leaves of wheat. Importantly, the level of ECs was observed to be dependent on the age of the plants, as well as on growth conditions (especially temperature). Fenarimol, an inhibitor of a cytochrome P450 monooxygenase, was shown to significantly decrease the level of naturally occurring ECs in experimental plants, which may indicate its potential use in studies related to the biosynthesis and physiological function of these substances in plants.

Application of natural and synthetic polyamines as growth regulators to improve the freezing tolerance of winter wheat (Triticum aestivum L.)

2012 ◽  
Vol 60 (1) ◽  
pp. 1-10 ◽  
Author(s):  
D. Todorova ◽  
I. Sergiev ◽  
V. Alexieva
Keyword(s):  
Winter Wheat ◽  
Foliar Application ◽  
Normal Growth ◽  
Triticum Aestivum L ◽  
Growth Conditions ◽  
Freezing Stress ◽  
Preliminary Treatment ◽  
Fresh Weight ◽  
Soil Culture ◽  
Weight Losses

Wheat cultivars were grown as soil culture under normal growth conditions. Twoweek- old seedlings were exposed to 4°C for 6 h and then transferred to −12°C for 24 h in the dark. Twenty-four hours before freezing stress, some of the plants were sprayed with aqueous solutions of spermine, spermidine, putrescine, 1,3-diaminopropane (1,3-DAP) and diethylenetriamine (DETA). The data showed that freezing stress caused a decrease in the fresh weight, chlorophyll content and plant survival rate, accompanied by a simultaneous accumulation of free proline and the enhanced leakage of electrolytes. Preliminary treatment with polyamines caused a decline in electrolyte leakage and a considerable augmentation in proline quantity, indicating that the compounds are capable of preventing frost injury. Additionally, the foliar application of polyamines retarded the destruction of chlorophyll, and lessened fresh weight losses due to freezing stress. The synthetic triamine DETA was the most effective, having the most pronounced action in all the experiments, followed by the tetraamine spermine. The application of polyamines to wheat crops could be a promising approach for improving plant growth under unfavourable growth conditions, including freezing temperatures. The results demonstrate that treatment with polyamines could protect winter wheat by reducing the stress injuries caused by subzero temperatures.

The Role of Leaf Epicuticular Wax in the Adaptation of Wheat ( Triticum aestivum L.) to High Temperatures and Moisture Deficit Conditions

Crop Science ◽  
2018 ◽  
Vol 58 (2) ◽  
pp. 679-689 ◽  
Author(s):  
Suheb Mohammed ◽  
Trevis D. Huggins ◽  
Francis Beecher ◽  
Chris Chick ◽  
Padma Sengodon ◽  
...  
Keyword(s):  
Triticum Aestivum ◽  
High Temperatures ◽  
Triticum Aestivum L ◽  
Epicuticular Wax ◽  
Moisture Deficit ◽  
Leaf Epicuticular Wax

scholarly journals Lr19 Resistance in Wheat Becomes Susceptible to Puccinia triticina in India

Plant Disease ◽  
2005 ◽  
Vol 89 (12) ◽  
pp. 1360-1360 ◽  
Author(s):  
S. C. Bhardwaj ◽  
M. Prashar ◽  
S. Kumar ◽  
S. K. Jain ◽  
D. Datta
Keyword(s):  
Leaf Rust ◽  
Wheat Variety ◽  
Puccinia Triticina ◽  
Triticum Aestivum L ◽  
Wheat Breeding ◽  
Adult Plant ◽  
Distribution Data ◽  
Agropyron Elongatum ◽  
Peninsular India ◽  
Slow Rusting

Lr19, a resistance gene originally transferred from Agropyron elongatum to wheat (Triticum aestivum L.), has remained effective worldwide against leaf rust (Puccinia triticina Eriks.) except in Mexico (1). This report records a new pathotype of P. triticina virulent on Lr19 from India. From 2003 to 2004, 622 wheat leaf rust samples from 14 states were subjected to pathotype analysis. Samples were established on susceptible wheat cv. Agra Local, and pathotypes were identified on three sets of differentials following binomial nomenclature (3). Virulence on Lr19 (Agatha T4 line) was observed in approximately 2% of samples. These samples were picked from Lr19 (NIL), cvs. Ajit, Lal Bahadur, Local Red, Lok1, and Nirbhay from Karnataka and Gujarat states. All Lr19 virulent isolates were identical. The reference culture is being maintained on susceptible wheat cv. Agra Local and has also been put under long-term storage in a national repository at Flowerdale. From 2004 to 2005, this pathotype was detected in 6.3% of samples from central and peninsular India. There is no wheat variety with Lr19 under cultivation in India, however, it is being used in wheat breeding programs targeted at building resistance against leaf and stem rusts. NIL's Lr19/Sr25 (LC25) and Lr19/Sr25 (82.2711) were also susceptible to this isolate, whereas Lr19/Sr25 (spring accession) was resistant. The new isolate, designated as 253R31 (77-8), appears to be close to the pathotype 109R31 (4) with additional virulence for Lr19. The avirulence/virulence formula of pathotype 253R31 is Lr9, 23, 24, 25, 26, 27+31, 28, 29, 32, 36, 39, 41, 42, 43, 45/Lr1, 2a, 2b, 2c, 3, 10, 11, 12, 13, 14a, 14b, 14ab, 15, 16, 17, 18, 20, 21, 22a, 22b, 30, 33, 34, 35, 37, 38, 40, 44, 48, and 49. To our knowledge, this is the first report of virulence on Lr19 from two states of India. On international rust differentials, it is designated as TGTTQ (2), and is different from CBJ/QQ (1), the other isolate reported virulent on Lr19 from Mexico. The Mexican isolate is avirulent on Lr1, 2a, 2b, 2c, 3ka, 16, 21, and 30 to which the Indian isolate is virulent. However, both isolates are avirulent on Lr9, 24, 26, 36, and Lr42. Among the wheat cultivars identified during the last 6 years, HD2824, HD2833, HD2864, HI1500, HS375, HUW 510, HW 2044, HW 5001, Lok 45, MACS 6145, MP4010, NW 2036, PBW 443, PBW 498, PBW 502, PBW 524, Raj 4037, UP 2565, VL 804, VL 829, and VL 832 and lines of wheat possessing Lr9, Lr23, Lr24, and Lr26 showed resistance to this pathotype. PBW 343, which occupies more than 5 million ha in India, is also resistant to this pathotype along with PBW 373. An integrated strategy using a combination of diverse resistance genes, deployment of cultivars by using pathotype distribution data, slow rusting, and adult plant resistance is in place to curtail selection of new pathotypes and prevent rust epiphytotics. References: (1) J. Huerta-Espino and R. P. Singh. Plant Dis. 78:640,1994. (2) D. V. Mc Vey et al. Plant Dis. 88:271, 2004. (3) S. Nagarajan et al. Curr. Sci. 52:413, 1983. (4) S. K. Nayar et al. Curr. Sci. 44:742, 1975.

scholarly journals Karakterisasi Morfologi dan Sifat Kuantitatif Gandum (Triticum aestivum L.) di Dataran Menengah

2016 ◽  
Vol 44 (2) ◽  
pp. 162
Author(s):  
Sartika Widowati ◽  
Nurul Khumaida ◽  
Sintho Wahyuning Ardie ◽  
Dan Trikoesoemaningtyas
Keyword(s):  
Morphological Character ◽  
Plant Biomass ◽  
Block Design ◽  
Triticum Aestivum L ◽  
Morphological Characters ◽  
Environmental Condition ◽  
Harvest Time ◽  
Wheat Growth ◽  
Randomized Complete Block Design ◽  
Weight Number

ABSTRACT<br /><br />Indonesia is one of the largest wheat importers. Suitable environmental condition for wheat needs to be studied if wheat is going to be widely cultivated in Indonesia. The adaptability of wheat grown in various climates and altitudes is one of the important aspects. The objective of this experiment was to study the quantitative and morphological character of wheat grown in middle land (540 m asl) in Bogor, West Java. The experiment was arranged in randomized complete block design with three replications. Wheat genotypes used were three national varieties (Nias, Selayar, and Dewata), four new improved varieties (Guri 3 Agritan, Guri 4 Agritan, Guri 5 Agritan, and Guri 6 Unand), and one introduced genotype (SBD). Data were collected for several quantitative variables and seventeen morphological characters based on UPOV descriptor. The result showed that wheat growth was restricted in Bogor. Genotype determined plant height, leaf number, ear length, root length, number of spikelet, harvest time, seed weight, number of tillers, and plant biomass. Based on ear length, grain weight, and plant biomass, Guri 3 Agritan had the highest production than the other genotypes.<br /><br />Keywords: diversity, genetic relationship, high temperature, introduced genotype, phylogenetic<br /><br />

Influence of High Temperatures on the Mechanical Properties of Wood Bio-Concretes

2022 ◽  
Author(s):  
Amanda Lorena Dantas Aguiar ◽  
M’hamed Yassin Rajiv da Gloria ◽  
Romildo Dias Toledo Filho
Keyword(s):  
Mechanical Properties ◽  
Compressive Strength ◽  
High Temperatures ◽  
Room Temperature ◽  
Plant Biomass ◽  
Compression Tests ◽  
Wood Degradation ◽  
Cementitious Matrix ◽  
Wood Shavings ◽  
Temperature Reference

The use of wood wastes in the production of bio-concrete shows high potential for the development of sustainable civil construction, since this material, in addition to having low density, increases the energy efficiency of buildings in terms of thermal insulation. However, a concern arising from the production of bio-concretes with high amounts of plant biomass is how this material behaves when subjected to high temperatures. Therefore, this work aims to evaluate the influence of high temperatures on the mechanical properties of wood bio-concretes. The mixtures were produced with wood shavings volumetric fractions of 40, 50 and 60% and cementitious matrix composed of a combination of cement, fly ash and metakaolin. Uniaxial compression tests and scanning electron microscopy (SEM) were performed, with bio-concrete at age of 28 days, at room temperature (reference) and after exposure to temperatures of 100, 150, 200 and 250 °C. The density and compressive strength of the bio-concrete gradually decreased with increasing biomass content. Up to 200 °C, reductions in strength and densities less than 19% and 13%, respectively, were observed. At 250 °C, reductions of compressive strength reached 87%. Analysis performed by SEM showed an increase in the number of cracks in the wood-cementitious matrix interface and wood degradation by increasing the temperature.

scholarly journals Affordable Phenotyping of Winter Wheat under Field and Controlled Conditions for Drought Tolerance

Agronomy ◽  
2020 ◽  
Vol 10 (6) ◽  
pp. 882
Author(s):  
Dhananjay Kumar ◽  
Sandeep Kushwaha ◽  
Chiara Delvento ◽  
Žilvinas Liatukas ◽  
Vivekanand Vivekanand ◽  
...  
Keyword(s):  
Drought Tolerance ◽  
Winter Wheat ◽  
Growth Rates ◽  
Plant Biomass ◽  
Growth Conditions ◽  
Seedling Stage ◽  
Growth Stages ◽  
Digital Cameras ◽  
Relative Growth ◽  
Relative Growth Rates

Drought stress is one of the key plant stresses reducing grain yield in cereal crops worldwide. Although it is not a breeding target in Northern Europe, the changing climate and the drought of 2018 have increased its significance in the region. A key challenge, therefore, is to identify novel germplasm with higher drought tolerance, a task that will require continuous characterization of a large number of genotypes. The aim of this work was to assess if phenotyping systems with low-cost consumer-grade digital cameras can be used to characterize germplasm for drought tolerance. To achieve this goal, we built a proximal phenotyping cart mounted with digital cameras and evaluated it by characterizing 142 winter wheat genotypes for drought tolerance under field conditions. The same genotypes were additionally characterized for seedling stage traits by imaging under controlled growth conditions. The analysis revealed that under field conditions, plant biomass, relative growth rates, and Normalized Difference Vegetation Index (NDVI) from different growth stages estimated by imaging were significantly correlated to drought tolerance. Under controlled growth conditions, root count at the seedling stage evaluated by imaging was significantly correlated to adult plant drought tolerance observed in the field. Random forest models were trained by integrating measurements from field and controlled conditions and revealed that plant biomass and relative growth rates at key plant growth stages are important predictors of drought tolerance. Thus, based on the results, it can be concluded that the consumer-grade cameras can be key components of affordable automated phenotyping systems to accelerate pre-breeding for drought tolerance.

scholarly journals Genome-Wide Investigation of Heat Shock Transcription Factor Family in Wheat (Triticum aestivum L.) and Possible Roles in Anther Development

2020 ◽  
Vol 21 (2) ◽  
pp. 608 ◽  
Author(s):  
Jiali Ye ◽  
Xuetong Yang ◽  
Gan Hu ◽  
Qi Liu ◽  
Wei Li ◽  
...  
Keyword(s):  
Heat Shock ◽  
Phylogenetic Analyses ◽  
Expression Patterns ◽  
Heat Shock Protein 90 ◽  
Triticum Aestivum L ◽  
Wheat Breeding ◽  
Anther Development ◽  
Male Sterile ◽  
Specific Expression ◽  
Genome Wide

Heat shock transcription factors (HSFs) play crucial roles in resisting heat stress and regulating plant development. Recently, HSFs have been shown to play roles in anther development. Thus, investigating the HSF family members and identifying their protective roles in anthers are essential for the further development of male sterile wheat breeding. In the present study, 61 wheat HSF genes (TaHsfs) were identified in the whole wheat genome and they are unequally distributed on 21 chromosomes. According to gene structure and phylogenetic analyses, the 61 TaHsfs were classified into three categories and 12 subclasses. Genome-wide duplication was identified as the main source of the expansion of the wheat HSF gene family based on 14 pairs of homeologous triplets, whereas only a very small number of TaHsfs were derived by segmental duplication and tandem duplication. Heat shock protein 90 (HSP90), HSP70, and another class of chaperone protein called htpG were identified as proteins that interact with wheat HSFs. RNA-seq analysis indicated that TaHsfs have obvious period- and tissue-specific expression patterns, and the TaHsfs in classes A and B respond to heat shock, whereas the C class TaHsfs are involved in drought regulation. qRT-PCR identified three TaHsfA2bs with differential expression in sterile and fertile anthers, and they may be candidate genes involved in anther development. This comprehensive analysis provides novel insights into TaHsfs, and it will be useful for understanding the mechanism of plant fertility conversion.

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