scholarly journals A Zoospore Inoculation Method with Phytophthora sojae to Assess the Prophylactic Role of Silicon on Soybean Cultivars

Plant Disease ◽  
2014 ◽  
Vol 98 (12) ◽  
pp. 1632-1638 ◽  
Author(s):  
Valérie Guérin ◽  
Amandine Lebreton ◽  
Erik E. Cogliati ◽  
Sue E. Hartley ◽  
François Belzile ◽  
...  
Keyword(s):  
Natural Infection ◽  
Infection Process ◽  
Phytophthora Sojae ◽  
Controlled Environment ◽  
Hydroponic System ◽  
Soybean Cultivars ◽  
Direct Inoculation ◽  
Soybean Plants ◽  
Root Resistance

The objective of this study was to evaluate whether silicon (Si) amendments, known to have a prophylactic role against biotrophic and hemibiotrophic pathogens, could protect soybean against Phytophthora sojae. To fulfill this objective, the initial challenge was to develop a method of inoculation that reproduced the natural infection process while allowing regular Si feeding to the plants. In a first set of experiments, inoculation of P. sojae zoospores directly into hydroponic solutions led to reproducible infections and expected phenotypes when using ‘Williams’ (rps), ‘L75-6141’ (Rps1a), ‘haro15’ (Rps1k), and ‘L77-1863’ (Rps1b) soybean challenged to races 3 and 7 of P. sojae. This approach offers the advantage of testing simultaneously many soybean cultivars against different races of P. sojae in a controlled environment, and the expression of partial and root resistance. In a second set of experiments aimed at testing the effect of Si, our results clearly showed that Si amendments had a significant effect on disease reduction and plant yield. The effect was particularly noticeable when combined with a cultivar displaying a certain level of resistance to the disease. These results demonstrate a useful method of direct inoculation of soybean plants with P. sojae zoospores through a hydroponic system and show that Si amendments can represent an alternative method of control of P. sojae against soybean.

Silicon influences the localization and expression of Phytophthora sojae effectors in interaction with soybean

2020 ◽  
Vol 71 (21) ◽  
pp. 6844-6855 ◽  
Author(s):  
Aliyeh Rasoolizadeh ◽  
Parthasarathy Santhanam ◽  
Caroline Labbé ◽  
Sheelavanta Matha Shivaraj ◽  
Hugo Germain ◽  
...  
Keyword(s):  
Infection Process ◽  
Phytophthora Sojae ◽  
Host Recognition ◽  
Fluorescence Labeling ◽  
Post Inoculation ◽  
Soybean Plants ◽  
Effector Recognition ◽  
Plant Pathogen Interactions ◽  
Microscopy Techniques ◽  
Lower Expression

Abstract In plant–pathogen interactions, expression and localization of effectors in the aqueous apoplastic region play a crucial role in the establishment or suppression of pathogen development. Silicon (Si) has been shown to protect plants in several host–pathogen interactions, but its mode of action remains a source of debate. Its deposition in the apoplastic area of plant cells suggests that it might interfere with receptor–effector recognition. In this study, soybean plants treated or not with Si were inoculated with Phytophthora sojae and differences in the ensuing infection process were assessed through different microscopy techniques, transcript analysis of effector and defense genes, and effector (Avr6) localization through immunolocalization and fluorescence labeling. In plants grown without Si, the results showed the rapid (4 d post-inoculation) host recognition by P. sojae through the development of haustorium-like bodies, followed by expression and release of effectors into the apoplastic region. In contrast, Si treatment resulted in limited pathogen development, and significantly lower expression and presence of Avr6 in the apoplastic region. Based on immunolocalization and quantification of Avr6 through fluorescence labeling, our results suggest that the presence of Si in the apoplast interferes with host recognition and/or limits receptor–effector interactions, which leads to an incompatible interaction.

scholarly journals Plant lipid metabolism in susceptible and tolerant soybean (Glycine max) cultivars in response to Phytophthora sojae colonization and infection

2021 ◽  
Author(s):  
Oludoyin Adeseun Adigun ◽  
Thu Huong Pham ◽  
Dmitry Grapov ◽  
Muhammad Nadeem ◽  
Linda Elizabeth Jewell ◽  
...  
Keyword(s):  
Lipid Metabolism ◽  
Plant Protection ◽  
Phytophthora Sojae ◽  
Lipid Biomarkers ◽  
Crop Loss ◽  
Susceptible Cultivar ◽  
Plant Lipid ◽  
Soybean Cultivars ◽  
Tolerance Response

Soybean is one of the most cultivated crops globally and a staple food for much of the world's population. The annual global crop losses due to infection by the Phytophthora sojae are currently estimated at approximately $2B USD, yet we have limited understanding of the role of lipid metabolism in the adaptative strategies used to limit infection and crop loss. We employed a multi-modal lipidomics approach to investigate how soybean cultivars remodel their lipid metabolism to successfully limit infection by Phytophthora sojae. Both the tolerant and susceptible soybean cultivars showed alterations in lipid metabolism in response to Phytophthora sojae infection. Relative to non-inoculated controls, induced accumulation of stigmasterol was observed in the susceptible cultivar whereas, induced accumulation of phospholipids and glycerolipids occurred in tolerant soybean cultivar. We have generated a comprehensive metabolic map of susceptible and tolerant soybean root and stem lipid metabolism to identify lipid modulators of host immune or tolerance response to Phytophthora sojae infection and identified potential pathways and unique lipid biomarkers like TG(15:0/22:0/22:5), TG(10:0/10:0/10:0), TG(10:0/10:0/14:0), DG(18:3/18:3), DG(16:0/18:3) and DG(24:0/18:2) as possible targets for the development of future plant protection solutions.

scholarly journals Identification of Resistance Genes to Phytophthora sojae in Domestic Soybean Cultivars from China Using Particle Bombardment

Plant Disease ◽  
2020 ◽  
Vol 104 (7) ◽  
pp. 1888-1893
Author(s):  
Jin Yang ◽  
Sujiao Zheng ◽  
Xiaomen Wang ◽  
Wenwu Ye ◽  
Xiaobo Zheng ◽  
...  
Keyword(s):  
Resistance Genes ◽  
Particle Bombardment ◽  
Phytophthora Sojae ◽  
Stem Rot ◽  
Avirulence Genes ◽  
Breeding Programs ◽  
Soybean Cultivars ◽  
Primary Means ◽  
Soybean Plants ◽  
Source Materials

Phytophthora root and stem rot caused by Phytophthora sojae is a destructive disease that afflicts soybean plants throughout the world. The use of resistant soybean cultivars is the primary means of managing this disease, as well as the most effective and economical approach. There are abundant soybean germplasm resources in China that could be deployed for breeding programs; however, the resistance genes (Rps genes) in most cultivars are unknown, leading to uncertainty concerning which are resistant cultivars for use. The resistance genes Rps1a, Rps1c, and Rps1k prevent root and stem rot caused by most P. sojae isolates within a Chinese field population. This study identified three Rps genes in Chinese domestic soybean cultivars using three related avirulence genes by particle bombardment. The complex genetic diversity of soybean cultivars and P. sojae strains has made it difficult to define single Rps genes without molecular involvement. Gene cobombardment is a method for identifying Rps genes quickly and specifically. We showed that cultivars Dongnong 60 and Henong 72 contained Rps1a, while Hedou 19, Henong 76, 75-3, Wandou 21020, Zheng 196, Wandou 28, Heinong 71, and Wandou 29 all contained Rps1c. The cultivars Jidou 12, Henong 72, Heinong 71, and Wandou 29 contained Rps1k. The cultivar Henong 72 contained both Rps1a and Rps1k, while Wandou 29 and Heinong 71 contained both Rps1c and Rps1k. We then evaluated the phenotype of 11 domestic soybean cultivars reacting to P. sojae using the isolates P6497 and Ps1. The 11 domestic cultivars were all resistant to P6497 and Ps1. This research provides source materials and parent plant strains containing Rps1a, Rps1c, and Rps1k for soybean breeding programs.

scholarly journals Plant lipid metabolism in response to Phytophthora sojae infection in susceptible and tolerant soybean cultivars

2021 ◽  
Author(s):  
Oludoyin Adigun ◽  
Thu Pham ◽  
Dmitry Grapov ◽  
Muhammad Nadeem ◽  
LINDA JEWELL ◽  
...  
Keyword(s):  
Lipid Metabolism ◽  
Plant Protection ◽  
Phytophthora Sojae ◽  
Lipid Biomarkers ◽  
Crop Loss ◽  
Susceptible Cultivar ◽  
Plant Lipid ◽  
Soybean Cultivars ◽  
Tolerance Response

Abstract Soybean is one of the most cultivated crops globally and a staple food for much of the world's population. The annual global crop losses due to infection by the Phytophthora sojae are currently estimated at approximately $2B USD, yet we have limited understanding of the role of lipid metabolism in the adaptative strategies used to limit infection and crop loss. We employed a multi-modal lipidomics approach to investigate how soybean cultivars remodel their lipid metabolism to successfully limit infection by Phytophthora sojae. Both the tolerant and susceptible soybean cultivars showed alterations in lipid metabolism in response to Phytophthora sojae infection. Relative to non-inoculated controls, induced accumulation of stigmasterol was observed in the susceptible cultivar whereas, induced accumulation of phospholipids and glycerolipids occurred in tolerant soybean cultivar. We have generated a comprehensive metabolic map of susceptible and tolerant soybean root and stem lipid metabolism to identify lipid modulators of host immune or tolerance response to Phytophthora sojae infection and identified potential pathways and unique lipid biomarkers like TG(15:0/22:0/22:5), TG(10:0/10:0/10:0), TG(10:0/10:0/14:0), DG(18:3/18:3), DG(16:0/18:3) and DG(24:0/18:2) as possible targets for the development of future plant protection solutions.

scholarly journals Thromboses and Hemostasis Disorders Associated with Coronavirus Disease 2019: The Possible Causal Role of Cross-Reactivity and Immunological Imprinting

2021 ◽  
Author(s):  
Darja Kanduc
Keyword(s):  
Immune System ◽  
Severe Acute Respiratory Syndrome ◽  
Natural Infection ◽  
Vascular Diseases ◽  
Cross Reactivity ◽  
Active Immunization ◽  
Thromboembolic Complications ◽  
Human Proteins ◽  
Almost All

AbstractBy examining the issue of the thromboses and hemostasis disorders associated with severe acute respiratory syndrome-coronavirus-2 (SARS-CoV-2) through the lens of cross-reactivity, it was found that 60 pentapeptides are shared by SARS-CoV-2 spike glycoprotein (gp) and human proteins that— when altered, mutated, deficient or, however, improperly functioning— cause vascular diseases, thromboembolic complications, venous thrombosis, thrombocytopenia, coagulopathies, and bleeding, inter alia. The peptide commonality has a relevant immunological potential as almost all of the shared sequences are present in experimentally validated SARS-CoV-2 spike gp-derived epitopes, thus supporting the possibility of cross-reactions between the viral gp and the thromboses-related human proteins. Moreover, many of the shared peptide sequences are also present in pathogens to which individuals have previously been exposed following natural infection or vaccinal routes, and of which the immune system has stored imprint. Such an immunological memory might rapidly trigger anamnestic secondary cross-reactive responses of extreme affinity and avidity, in this way explaining the thromboembolic adverse events that can associate with SARS-CoV-2 infection or active immunization.

scholarly journals Nanoparticles as Adjuvants and Nanodelivery Systems for mRNA-Based Vaccines

Pharmaceutics ◽  
2020 ◽  
Vol 13 (1) ◽  
pp. 45
Author(s):  
Iman M. Alfagih ◽  
Basmah Aldosari ◽  
Bushra AlQuadeib ◽  
Alanood Almurshedi ◽  
Mariyam M. Alfagih
Keyword(s):  
Messenger Rna ◽  
Immune Cell ◽  
Natural Infection ◽  
Immunological Response ◽  
Infection Process ◽  
Dual Function ◽  
Therapeutic Vaccines ◽  
Non Invasive ◽  
Recent Developments

Messenger RNA (mRNA)-based vaccines have shown promise against infectious diseases and several types of cancer in the last two decades. Their promise can be attributed to their safety profiles, high potency, and ability to be rapidly and affordably manufactured. Now, many RNA-based vaccines are being evaluated in clinical trials as prophylactic and therapeutic vaccines. However, until recently, their development has been limited by their instability and inefficient in vivo transfection. The nanodelivery system plays a dual function in RNA-based vaccination by acting as a carrier system and as an adjuvant. That is due to its similarity to microorganisms structurally and size-wise; the nanodelivery system can augment the response by the immune system via simulating the natural infection process. Nanodelivery systems allow non-invasive mucosal administration, targeted immune cell delivery, and controlled delivery, reducing the need for multiple administrations. They also allow co-encapsulating with immunostimulators to improve the overall adjuvant capacity. The aim of this review is to discuss the recent developments and applications of biodegradable nanodelivery systems that improve RNA-based vaccine delivery and enhance the immunological response against targeted diseases.

TOLERANCE OF EARLY-MATURING SOYBEAN CULTIVARS TO METRIBUZIN

1986 ◽  
Vol 66 (1) ◽  
pp. 125-130 ◽  
Author(s):  
G. H. FRIESEN ◽  
D. A. WALL
Keyword(s):  
Sandy Loam ◽  
Controlled Environment ◽  
Clay Loam ◽  
Clay Loam Soil ◽  
Soybean Cultivars ◽  
Early Maturing ◽  
Glycine Max L ◽  
Loam Soil ◽  
Environment Chamber ◽  
Chamber Studies

McCall, Maple Presto, Maple Amber and OT80-3 soybean (Glycine max (L.) Merr.) cultivars were evaluated under field conditions for their response to metribuzin. Maple Amber was found to be less tolerant than the other cultivars. In controlled environment chamber studies, injury to this cultivar was more severe on a sandy loam soil than on a clay loam soil. Fall applications of metribuzin, alone or tank-mixed with trifluralin, were tolerant to Maple Amber soybeans and such applications may offer a practical alternative to spring treatments for broad spectrum weed control in the less tolerant soybean cultivars grown in Manitoba.Key words: Metribuzin, trifluralin, preplant incorporation, fall treatments, soybean cultivars

scholarly journals Patterns of Gene Expression Upon Infection of Soybean Plants by Phytophthora sojae

2004 ◽  
Vol 17 (10) ◽  
pp. 1051-1062 ◽  
Author(s):  
Pat Moy ◽  
Dinah Qutob ◽  
B. Patrick Chapman ◽  
Ian Atkinson ◽  
Mark Gijzen
Keyword(s):  
Gene Expression ◽  
Time Course ◽  
Phytophthora Sojae ◽  
Gene Microarray ◽  
Pathogenesis Related ◽  
Soybean Plants ◽  
Related Proteins ◽  
Phytoalexin Biosynthesis ◽  
Host Tissues ◽  
Mixed Samples

To investigate patterns of gene expression in soybean (Glycine max) and Phytophthora sojae during an infection time course, we constructed a 4,896-gene microarray of host and pathogen cDNA transcripts. Analysis of rRNA from soybean and P. sojae was used to estimate the ratio of host and pathogen RNA present in mixed samples. Large changes in this ratio occurred between 12 and 24 h after infection, reflecting the rapid growth and proliferation of the pathogen within host tissues. From the microarray analysis, soybean genes that were identified as strongly upregulated during infection included those encoding enzymes of phytoalexin biosynthesis and defense and pathogenesis-related proteins. Expression of these genes generally peaked at 24 h after infection. Selected lipoxygenases and peroxidases were among the most strongly downregulated soybean genes during the course of infection. The number of pathogen genes expressed during infection reached a maximum at 24 h. The results show that it is possible to use a single microarray to simultaneously probe gene expression in two interacting organisms. The patterns of gene expression we observed in soybean and P. sojae support the hypothesis that the pathogen transits from biotrophy to necrotrophy between 12 and 24 h after infection.

scholarly journals The Effect of Rhizobium japonicum on the Growth of Soybean Cultivars in Coastal Area

2019 ◽  
Vol 4 (1) ◽  
pp. 33
Author(s):  
Okti Purwaningsih ◽  
C. Tri Kusumastuti ◽  
Y. Sulistyo Nugroho ◽  
Casper Yoda Morib
Keyword(s):  
Coastal Area ◽  
Root Nodule ◽  
Agricultural Land ◽  
Dry Weight ◽  
Rhizobium Japonicum ◽  
Soybean Cultivars ◽  
Randomized Design ◽  
Two Factors ◽  
Cultivation Technology ◽  
Soybean Plants

The natural resources of the coastal area in Indonesia have the potential to be developed as an agricultural land with the support of both cultivation technology and land processing,  which one of them was done by using Rhizobium japonicum bacteria to meet the need of nitrogen in the soybean plants. This study aimed to determine the characteristics of nitrogen fixation in various soybean cultivars planted in the coastal area. The research was conducted in Mancingan, Parangtritis, Kretek, Bantul, DIY. The study was designed in Completely Randomized Design which consisted of two factors and was repeated three times. Factor I was Rhizobium japonicum inoculation (with inoculation and without inoculation); factor II was 10 various cultivars of soybean (Grobogan, Burangrang, Argomulyo, Anjasmara, Dena 1, Gema, Kaba, Wilis, Sinabung, Gepak Kuning). The results showed that the inoculation of Rhizobium japonicum in soybean cultivars in a coastal area could increase the number of root nodule, dry weight of root nodule, dry seed weight per plant, and harvest index. Burangrang cultivar planted in the coastal area was the most responsive to Rhizobium japonicum inoculation among other soybean cultivars tested.

scholarly journals Relevance of animal infections in SARS-Cov-2 spread: an update after 1 year of pandemic.

2021 ◽  
Author(s):  
Frazzini Sara ◽  
Amadori Massimo ◽  
Lauretta Turin ◽  
Federica Riva
Keyword(s):  
Animal Species ◽  
Natural Infection ◽  
Farm Animals ◽  
World Health ◽  
Chinese City ◽  
Experimental Infections ◽  
Novel Coronavirus ◽  
Health Organization ◽  
Therapy Studies

In December 2019, several cases of pneumonia caused by a novel Coronavirus, later identified as SARS-CoV-2, were detected in the Chinese city of Wuhan. Due to its rapid, worldwide spread, on 11 March 2020 the World Health Organization declared a pandemic state. Since this new virus is genetically similar to the coronaviruses of bats, it was thought to have a zoonotic origin. Within a year of the appearance of SARS-CoV-2, several cases of infection were also reported in animals, suggesting animal-to-human and animal-to-animal transmission within mammals. Natural infection has been found in both companion and captive animals such as lions, tigers and gorillas. Among farm animals, the only ones found to be susceptible to SARS-CoV-2 infection so far are minks. Experimental infections have documented the susceptibility to SARS-CoV-2 of several animal species, such as humanized mice, hamsters, cats, dogs, ferrets, racoon dogs, cattle and non-human primates. Experimental infections are crucial for both elucidation of the role of animals in transmission and development of appropriate animal models for pathogenesis and therapy studies. This review aims to update the knowledge on natural and experimental SARS-CoV-2 infections in animals.

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