scholarly journals Reduced Meloidogyne ethiopica Parasitism in Vitis vinifera Grafted on Six Resistant Rootstocks Under Field and Greenhouse Conditions

Plant Disease ◽  
2017 ◽  
Vol 101 (6) ◽  
pp. 924-928
Author(s):  
J. C. Magunacelaya ◽  
R. Mancilla ◽  
S. González-Bernal

Meloidogyne ethiopica, an aggressive nematode, causes significant economic losses to Vitis crops. Rootstocks can successfully manage phytoparasitic nematodes. However, no studies exist on M. ethiopica-resistant rootstocks under field conditions. This study assessed the resistance of six Vitis rootstocks to M. ethiopica under field and greenhouse conditions. The number of galls and eggs in vine roots, quantity of second stage juveniles and males in 250 ml of soil, root weight, and shoot weight were determined for the Harmony, SO4, 101-14 MG, 110R, 3309C, and Kober 5BB rootstocks, and the own-root Chardonnay variety as a control. In the field, Harmony, SO4, 101-14 MG, Kober 5BB, and 110R were highly resistant to nematode parasitism and reproduction. In turn, 3309C was the least resistant rootstock. In the greenhouse, all rootstocks similarly limited M. ethiopica attack and reproduction. In both conditions, Chardonnay was the most susceptible vine to nematode attack, with high quantities of galls, eggs, and nematode reproduction. In conclusion, most of the evaluated rootstocks reduced M. ethiopica parasitism. Harmony, SO4, 101-14 MG, 110R, and Kober 5BB rootstocks are options for vineyard use, with final selection dependent on winegrower requirements.

Nematology ◽  
2021 ◽  
pp. 1-6
Author(s):  
Juliana de O. Silva ◽  
Camilla M. Oliveira ◽  
Renê G. da S. Carneiro ◽  
Mara R. da Rocha

Summary Meloidogyne enterolobii is a species capable of overcoming plant resistance moderated by the Mi-1 gene, which is effective against most species of root-knot nematode. This study evaluated the effect of induced resistance in tomato plants (Solanum lycopersicum ‘H-9553’) with the Mi-1 gene against the development and reproduction of M. enterolobii. Seedlings of tomato ‘H-9553’ were transplanted into pots, inoculated with 2000 eggs and second-stage juveniles (J2) of M. enterolobii and treated with Acibenzolar-S-Methyl, Bacillus subtilis, B. subtilis + B. licheniformis + Trichoderma longibrachiatum and extract of Reynoutria sachalinensis. The plants were collected at 5, 10, 15, 20, 25 and 30 days after inoculation (DAI) for the analyses of nematode penetration and development, and at 30 DAI for nematode reproduction. The use of B. subtilis increased fresh root weight when compared to the other treatments (20 DAI). There was a reduction in penetration of J2 in the roots of plants subjected to different resistance inducers. The population density of M. enterolobii was significantly reduced only when plants were treated with R. sachalinensis, indicating it as a potential resistance-inducing agent in tomato plants.


2019 ◽  
Vol 11 (5) ◽  
pp. 70 ◽  
Author(s):  
Muhammad Nazir Uddin ◽  
Saifullah Saifullah ◽  
Musharaf Ahmad ◽  
Wajid Khan ◽  
Bilal Muhammad Khan

Restricted applications of chemical nematicides has directed to the development of ecofriendly alternatives. Culture filtrates in different concentrations (20, 40, 60, 80 and 100%) of the six isolates of Pochonia chlamydosporia were evaluated for their nematicidal activity. Significant variations were observed in the inhibitory activity of culture filtrates against egg hatching and second stage juveniles of M. incognita at different concentrations. The culture extract of PC-6 showed maximum inhibition of egg hatching followed by PC-1. On the other hand PC1 isolate caused high mortality of second stage juveniles followed by PC-6. Significant variation among the isolates was observed for parasitism of M. incognita juveniles and eggs. Among the tested isolates, PC-1 and PC-6 were selected for the further studies based on in vitro egg inhibition and larval mortality. Both PC1 and PC6 applied at the rate of 6 × 103 chlamydospores per gram of soil per plant reduced the population of nematode and improved the growth and productivity of plant under screen house and field conditions. Different growth parameters like plant height, fresh shoot weight, dry shoot weight, fresh root weight and dry root weight were measured. Efficacy of isolates was different in winter and summer crop. No significant difference among the treatments was observed in summer crop. However in winter crop, considerable effect of both isolates was observed. Fungal application was effective at the rate of 6 × 103 chlamydospores per gram of soil per plant in both winter and summer crops. Root coating with chlamydospores suspension significantly reduced number of galls on roots. Little effect of both isolates was observed with mycelial root coating. No lesions were seen on P. chlamydosporia inoculated roots of tomato plants.


2021 ◽  
Vol 16 (8) ◽  
pp. 75-80
Author(s):  
Pitchaiah Pelapudi ◽  
Sasikala Ch ◽  
Swarnabala Ganti

In the present rapid growing world, need for a sustainable agricultural practice which helps in meeting the adequate food demand is much needed. In this context, plant growth promoting bacteria were brought into the spot light by the researchers. Though the plant growth promoting bacteria have several beneficial applications, due to some of the disadvantages in the field conditions, they lagged behind. In the current research work, native PGPR were isolated from the rhizosphere soil samples of maize with an aim to isolate the nitrogen fixing, phosphate solubilising and potash solubilising bacteria. Out of the several isolates, potent PGPR isolates viz., Paenibacillus durus PCPB067, Bacillus megaterium PCBMG041 and Paenibacillus glucanolyticus PCPG051 were isolated and identified by using the 16 S rRNA gene sequencing studies. Genomic DNA sequences obtained were deposited in the NCBI Genbank and accession numbers were assigned as MW793452, MW793456 and MW843633. In order to check the efficacy of the PGPR isolates, pot trials were conducted by taking maize as the host plant. Several parameters viz. shoot length, shoot weight, root length, root weight and weight of the seeds were tested in which PGP treatment showed good results (shoot length - 187±3.5 cm, shoot weight - 31±4 g, root length - 32±3.6 cm, root weight - 17±2 g, yield- 103.3±6.1 g) when compared to the chemical fertilizer treatment (shoot length - 177±3.5 cm, shoot weight - 25±3.6 g, root length - 24±3.5 cm, root weight - 14.6±1.52 g, yield- 85.6±7.6 g). Based on the results, it can be stated that these native PGPR isolates can be effectively used in the plant growth promotion of maize.


1970 ◽  
Vol 9 ◽  
pp. 21-27 ◽  
Author(s):  
Nabin Kumar Dangal ◽  
D. Sharma Poudyal ◽  
S. M. Shrestha ◽  
C. Adhikari ◽  
J. M. Duxbury ◽  
...  

Pot experiment was conducted during July-September 2006 to evaluate some organic amendments such as sesame (Sesamum indicum) biomass, buckwheat (Fagopyrum esculentum) biomass, neem (Azadirachta indica) leaves, chinaberry (Melia azedarch) leaves and chicken manure @ 1, 2 and 3 t ha-1 each against the rice root-knot nematode (Meloidogyne graminicola Golden & Birchfield) in direct seeded rice. The treatments were replicated five times in a randomized complete block design. The number of second stage juveniles (J2) of M. graminicola was significantly low in chicken manure @ 3 t ha-1. The root knot severity index was significantly low in sesame @ 3 t ha-1, chinaberry @ 3, 2 or 1 t ha-1, neem @ 3 t ha-1 and chicken manure @ 2 or 3 t ha-1 amended soil but root lesion severity index was lower only in chicken manure @ 2 t ha-1 treated plots. The fresh shoot weight and length were significantly high in chicken manure amendment @ 2 or 3 t ha-1 at 45th day after seeding. However, the fresh root weight, length, number of leaves and number of J2 recovered from the roots were non-significant. Key words: biomass; juveniles; Meloidogyne graminicola; root-knot severity index; root lesion severity index DOI: 10.3126/njst.v9i0.3160 Nepal Journal of Science and Technology 9 (2008) 21-27


Genetika ◽  
2012 ◽  
Vol 44 (2) ◽  
pp. 235-250 ◽  
Author(s):  
Ahmad Dadashpour

The effects of different salt sources (C Cl2, NaCl, and KCl) and concentrations, as measured by electrical conductivity, (0, "control", 1, 3, 5, 7 and 9 dS m-1) on seed germination and seedling growth of ?Ferro?, ?Obez?, ?RS 841? and ?Strong Tosa F1? pumpkin varieties used as rootstock were investigated in this study. The results showed that germination rate, root length, shoot length, fresh root weight, dry root weight, fresh shoot weight and dry shoot weights tend to decrease when the electrical conductivity of the solution is higher than 5 dS m-1, independent of salt sources and in all of the varieties. Three days after seeding, a germination ratio of 5 % was obtained from RS 841 variety in all salt source and concentrations, while a germination ratio over 50 % was obtained in ?Strong Tosa? variety for the same conditions except CaCl2 salt source. Nevertheless, seeds germinated in medium having high concentrations of CaCl2 had lower germination rate and poor seedling growth, compared to media having the same concentrations of NaCl and KCl. It was concluded that all of the varieties studied were more sensitive to the concentrations prepared using CaCl2 than that of the KCl, and NaCl.


Author(s):  
Mahmut Çamlıca ◽  
Gülsüm Yaldız ◽  
Ferit Özen ◽  
Abdurrahman Başol ◽  
Halit Aşkın

Sage and mountain sage belong to Lamiaceae family which have commercial importance from medicinal and aromatic plants in Turkey. The aim of this study was to evaluate the effects of different doses of selenium in salinity conditions on the morphological characteristics of sage (Salvia officinalis L.) and mountain tea (Sideritis sp.). Four different doses of selenium (5, 10, 20, 40 mg/l) were applied in without salinity (0) and salinity (250 mM NaCl/l) medium. The experiment was carried out in the split plot design with three replications in the climate chamber room of the department of field crops. When the examined properties were evaluated; plant height changed between 15.56-23.85 cm, number of branches 10.50-12.78 number/plant, number of leaves 52.78-92.00 number/plant, fresh leaf weight 2.48-7.51 g/plant, dry leaf weight 0.48-3.32 g/plant, fresh root weight 1.52-7.16 g/plant, dry root weight 0.19-1.24 g/plant, root length 26.18-36.07 cm, fresh shoot weight of 1.13-7.15 g/plant and dry shoot weight 0.13-0.38 g/plant. In mountain tea, the properties were determined for plant height as 3.26-5.93 cm, for number of branches as 2.50-6.33 number/plant, for number of leaves as 28.22-91.14 number/plant, for fresh and dry leaf weights as 2.42-11,03 and 0.45-1.91 g/plant, for fresh and dry root weights as 0.71-3.97 and 0.18-0.74 g/plant, for root length as 14.78-33.26 cm, for fresh and dry shoot weights as 0.29-2.28 and 0.12-0.41 g/plant were determined. As a result of this study, 5 mg/l selenium application in salinity conditions in both plants has reached high values in terms of fresh leaf weights. In addition to this, it was found that selenium applications in salinity conditions had positive effects on dry leaf weight in sage and fresh root and fresh shoot weights in mountain tea. As a result of correlation analysis, positive correlations were found between dry leaf weight and other characteristics especially in mountain tea in terms of traits examined in both plants.


2017 ◽  
Vol 12 (2) ◽  
pp. 345-354
Author(s):  
Hemant Kumar ◽  
S. B. Lal ◽  
A. M. Wani

Estimation of phenotypic and genotypic correlation between various traits provides necessary information in breeding program, when selection is based on two or more characters. The intensity and trend of association among characters may be estimated by genetic coefficients of correlations and genotypic and phenotypic coefficient of correlation depending on the type of materials studied. The knowledge of genetic inter-relationship between characters is also of theoretical and practical interest because a genotypic correlation may drive genetic linkage or from developmental induced relationships between components that are only indirectly the consequences of gene action. Terminalia arjuna is a medium to large sized fast growing versatile tree popularly known as Arjun. It has immense economic potential in various industries viz., pharmaceutical, timber, paper, soap, tannin, dye, match, food, fodder and fuel. Thirty candidate plus trees (CPTs) of T. arjuna were selected as seed source from 30 different provinces of Uttar Pradesh for the study of correlation study morphological & biomass traits. After 12 months of growth under nursery condition, data were collected and analyze for association studies. Present study concludes that magnitudes of phenotypic correlation coefficients were lower compare to corresponding genotypic values. A strong positive and highly significant phenotypic correlation with collar diameter, bark content, fresh shoot weight, dry shoot weight, fresh root weight, dry root weight and total biomass of seedling were exhibited. Therefore, these characters may be given proper emphasis during selection and breeding program of Arjun tree species where the amount of leaf, bark and biomass production is of immense importance.


Akta Agrosia ◽  
2018 ◽  
Vol 21 (2) ◽  
pp. 55-60
Author(s):  
Marwanto Marwanto ◽  
Nasiroh Nasiroh ◽  
Bambang G. Mucitro ◽  
Merakati Handajaningsih

The beneficial effects of manure on soil properties, growth, and crop productivity have promoted its use for replacing the application of N fertilizer. However, it is not well understood to what extent N fertilizer was able to be substituted by cow manure. Accordingly, this pot experiment aimed to compare the effect of inorganic N fertilizer application alone with that of the combined use of inorganic N fertilizer with cow manure based on the same amount of total N on growth parameters, yield attributes, and nitrogen (N) uptake of black rice. The experiment was conducted under a screen house condition in Agriculture Faculty, Bengkulu University located at 15 meters altitude above sea level during the summer season of 2015. There were six treatments viz. T1 = 100% N from urea + 0% N from cow manure (0.52 g N + 0.00 g cow manure) pot-1, T2 = 80% N from urea  + 20% N from cow manure  (0.42 g N + 9.55 g cow manure) pot-1, T3 = 60% N from urea + 40% N from cow manure (0.31 g N  + 19.10 g cow manure) pot-1, T4 = 40% N from urea + 60% N from cow manure (0.21 g N + 28.65 g cow manure) pot-1, T5 = 20% N from urea + 80% N from cow manure (0.10 g N+ 38.20 g cow manure) pot-1, and T6 = 0% N from urea + 100% N from cow manure (0.00 g N  + 47.75 g cow manure) pot-1. The amount of inorganic N fertilizer in the form of urea and cow manure applied was calculated based on the recommended rate of 115.00 kg ha-1 for N fertilizer and 10.50 ton ha-1 for cow manure. These treatments were arranged in a Completely Randomized Design and repeated three times. The results showed that the treatments significantly (P ?0.005) affected growth parameters as measured by plant height, the number of leaves, fresh shoot weight, fresh root weight, dry shoot weight, dry root weight, yield attributes as determined by the total number of tillers, the total number productive tillers, grain yield per pot, and N uptake. The highest values for all these variables were obtained in the treatment receiving recommended rate of urea only (100% N from urea + 0% N from cow manure as equivalence) and the lowest in the treatment receiving a100% N from cow manure (0% N from urea + 100% N from cow manure). However, combined treatments of cow manure and inorganic N fertilizer such as 80% N from urea  + 20% N from cow manure, 60% N from urea + 40% N from cow manure 40% N from urea + 60% N from cow manure showed a parity statistically with the treatment receiving 100% N from urea only in maintaining the values for all these variables. Overall, the combined use of inorganic N fertilizer (urea) and cow manure as an equivalence promoted growth and yield of black rice by improving N uptake. Keywords: integrated nutrient management, soil chemical property, Nitrogen uptake, combined fertilizer application, black rice


Weed Science ◽  
1993 ◽  
Vol 41 (4) ◽  
pp. 534-540 ◽  
Author(s):  
Ronald E. Jones ◽  
Robert H. Walker

Greenhouse and growth chamber experiments with potted plants were conducted to determine the effects of interspecific root and canopy interference, light intensity, and soil moisture on water uptake and biomass of soybean, common cocklebur, and sicklepod. Canopy interference and canopy plus root interference of soybean with common cocklebur increased soybean water uptake per plant and per unit leaf area. Root interference with soybean decreased common cocklebur water uptake per plant. Canopy interference of soybean with sicklepod increased soybean water uptake per unit leaf area, while root interference decreased uptake per plant. Combined root and canopy interference with soybean decreased water uptake per plant for sicklepod. Soybean leaf area and shoot weight were reduced by root interference with both weeds. Common cocklebur and sicklepod leaf area and shoot weight were reduced by root and canopy interference with soybeans. Only common cocklebur root weight decreased when canopies interfered and roots did not. The relationship between light intensity and water uptake per unit leaf area was linear in both years with water uptake proportional to light intensity. In 1991 water uptake response to tight was greater for common cocklebur than for sicklepod. The relationship between soil moisture level and water uptake was logarithmic. Common cocklebur water uptake was two times that of soybean or sicklepod at −2 kPa of pressure potential. In 1991 common cocklebur water uptake decreased at a greater rate than soybean or sicklepod in response to pressure potential changes from −2 to −100 kPa.


1991 ◽  
Vol 5 (2) ◽  
pp. 430-433 ◽  
Author(s):  
Marshall B. Wixson ◽  
David R. Shaw

Thirty soybean cultivars were grown in a hydroponic culture to observe differential tolerance to AC 263,222. Root length was reduced with 1.1 mM AC 263,222 in 18 of the 30 cultivars. ‘Bedford’, ‘Asgrow 5403’, and ‘Asgrow 6297’ were cultivars with a root length reduction of 30% or more, whereas roots of ‘DPL 506’, ‘RA 606’, and ‘Coker 6727’ were reduced less than 7%. Root weight was reduced in only 10 of the 30 cultivars. Asgrow 6297, ‘Bragg’, and ‘Narow’ root weights were reduced more than 20%. Shoot weight was reduced in 23 cultivars, and ‘Coker 6955’, ‘Braxton’, ‘Coker 485’, ‘Epps', and Coker 6727 shoots were reduced more than 25%. The most tolerant cultivars were ‘Centennial’, RA 606, ‘Tracy M’, ‘Johnston’, and ‘Hartz 7126’. The most sensitive cultivars to AC 263,222 with all response variables measured were Bedford, Asgrow 6297, Asgrow 5403, Coker 6955, Narow, Bragg, and Braxton. These cultivars all have ‘Jackson’ as a common parent. This suggests a genetic basis for AC 263,222 sensitivity in soybean.


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