scholarly journals Effects of Varying Environmental Conditions on Biological Control of Fusarium Wilt of Tomato by Nonpathogenic Fusarium spp.

2002 ◽  
Vol 92 (11) ◽  
pp. 1160-1166 ◽  
Author(s):  
Robert P. Larkin ◽  
Deborah R. Fravel
Keyword(s):  
Biological Control ◽  
Fusarium Wilt ◽  
Environmental Conditions ◽  
Growth Parameters ◽  
Disease Incidence ◽  
Organic Matter Content ◽  
Matter Content ◽  
Disease Development ◽  
Temperature Regimes ◽  
Infested Field

The influence of varying environmental and cropping conditions including temperature, light, soil type, pathogen isolate and race, and cultivar of tomato on biological control of Fusarium wilt of tomato by isolates of nonpathogenic Fusarium oxysporum (CS-20 and CS-24) and F. solani (CS-1) was evaluated in greenhouse and growth chamber experiments. Liquid spore suspensions (106/ml) of the biocontrol isolates were applied to soilless potting mix at the time of tomato seeding, and the seedlings were transplanted into pathogen-infested field soil 2 weeks later. Temperature regimes ranging from 22 to 32°C significantly affected disease development and plant physiological parameters. Biocontrol isolate CS-20 significantly reduced disease at all temperature regimes tested, yielding reductions of disease incidence of 59 to 100% relative to pathogen control treatments. Isolates CS-24 and CS-1 reduced disease incidence in the greenhouse and at high temperatures, but were less effective at the optimum temperature for disease development (27°C). Growing plants under shade (50% of full light) versus full light affected some plant growth parameters, but did not affect the efficacy of biocontrol of any of the three bio-control isolates. Isolate CS-20 effectively reduced disease incidence (56 to 79% reduction) in four different field soils varying in texture (sandy to clayey) and organic matter content (0 to 3.2%). Isolate CS-1 reduced disease in the sandy and loamy soils (49 to 66% reduction), but was not effective in a heavy clay soil. Both CS-1 and CS-20 were equally effective against all three races of the pathogen, as well as multiple isolates of each race (48 to 66% reduction in disease incidence). Both isolates, CS-1 and CS-20, were equally effective in reducing disease incidence (66 to 80% reduction) by pathogenic races 1, 2, and 3 on eight different tomato cultivars containing varying levels of inherent resistance to Fusarium wilt (susceptible, resistant to race 1, or resistant to races 1 and 2). These results demonstrate that both these Fusarium isolates, and particularly CS-20, can effectively reduce Fusarium wilt disease of tomato under a variety of environmental conditions and have potential for further development.

Biological control of Fusarium wilt of cucumber with nonpathogenic isolates of Fusarium oxysporum

1987 ◽  
Vol 33 (5) ◽  
pp. 349-353 ◽  
Author(s):  
T. C. Paulitz ◽  
C. S. Park ◽  
R. Baker
Keyword(s):  
Biological Control ◽  
Fusarium Oxysporum ◽  
Infection Rate ◽  
Fusarium Wilt ◽  
Disease Incidence ◽  
Infection Rates ◽  
Control Activity ◽  
Significant Difference ◽  
Cucumber Roots ◽  
Linear Regressions

Nonpathogenic isolates of Fusarium oxysporum were obtained from surface-disinfested, symptomless cucumber roots grown in two raw (nonautoclaved) soils. These isolates were screened for pathogenicity and biological control activity against Fusarium wilt of cucumber in raw soil infested with Fusarium oxysporum f. sp. cucumerinum (F.o.c.). The influence of three isolates effective in inducing suppressiveness and three ineffective isolates on disease incidence over time was tested. The effective isolates reduced the infection rate (R), based on linear regressions of data transformed to loge (1/1 – y). Effective isolate C5 was added to raw soil infested with various inoculum densities of F.o.c. In treatments without C5, the increase in inoculum densities of F.o.c. decreased the incubation period of wilt disease, but there was no significant difference in infection rate among the inoculum density treatments. Isolate C5 reduced the infection rate at all inoculum densities of F.o.c. Various inoculum densities of C5 were added to raw soils infested with 1000 cfu/g of F.o.c. In the first trial, infection rates were reduced only in the treatment with 10 000 cfu/g of C5; in the second trial, infection rates were reduced in treatments with 10 000 and 30 000 cfu/g of C5.

scholarly journals Fusarium wilt biocontrol and tomato growth stimulation, using endophytic bacteria naturally associated with Solanum sodomaeum and S. bonariense plants

2020 ◽  
Vol 30 (1) ◽  
Author(s):  
Rania Aydi-Ben-Abdallah ◽  
Hayfa Jabnoun-Khiareddine ◽  
Mejda Daami-Remadi
Keyword(s):  
Fusarium Wilt ◽  
Endophytic Bacteria ◽  
Biochemical Characterization ◽  
Growth Parameters ◽  
Disease Incidence ◽  
Main Body ◽  
Safe Alternative ◽  
Rdna Sequencing ◽  
Tomato Growth

Abstract Background Fusarium wilt biocontrol using endophytic microorganisms may represent a potentially attractive and environmentally safe alternative since endophytes could better limit disease incidence and severity through inhibition of the systemic fungus progress. Main body of the abstract Twenty-three endophytic bacterial isolates, naturally associated with Solanum sodomaeum and Solanum bonariense, were evaluated for their ability to control Fusarium wilt of tomato induced by Fusarium oxysporum f. sp. lycopersici (FOL) and to promote plant growth. Selected endophytic isolates were screened in vivo, using the root dipping and the culture substrate drenching methods. The most bioactive isolates were subjected to morphological and biochemical characterization and subsequent identification through 16S rDNA sequencing genes. Seven isolates (Stenotrophomonas maltophilia S23, S24, S26 and S28; Bacillus sp. SV81; Azotobacter chroococcum S11; and Serratia marcescens S14) were found to be the most efficient in reducing disease severity by 82–96% over control. Treatments with these isolates led to a significant enhancement in growth parameters, estimated at 45.5–61 and 24.2–70.5% than the control, in tomato plants infected or not with FOL, respectively. Diffusible and volatile metabolites released from bacterial cultures had significantly limited FOL radial growth. All isolates were positive for indole-3-acetic acid (IAA) production. S. marcescens S14, S. maltophilia S28, and Bacillus sp. SV81 exhibited a positive phosphate solubilization activity. Production of chitinase, protease, pectinase, and hydrogen cyanide were also investigated. Short conclusion This study clearly demonstrated that endophytic bacteria recovered from these 2 Solanum species can be explored as promising biocontrol agents active against FOL and are able to enhance tomato growth.

Integrated Management of Fusarium Wilt of Chickpea with Sowing Date, Host Resistance, and Biological Control

2004 ◽  
Vol 94 (9) ◽  
pp. 946-960 ◽  
Author(s):  
Blanca B. Landa ◽  
Juan A. Navas-Cortés ◽  
Rafael M. Jiménez-Díaz
Keyword(s):  
Fusarium Wilt ◽  
Seed Yield ◽  
Integrated Management ◽  
Disease Incidence ◽  
Seedling Emergence ◽  
Disease Onset ◽  
Sowing Date ◽  
Biocontrol Agents ◽  
Disease Development ◽  
Over Time

A 3-year experiment was conducted in field microplots infested with Fusarium oxysporum f. sp. ciceris race 5 at Córdoba, Spain, in order to assess efficacy of an integrated management strategy for Fusarium wilt of chickpea that combined the choice of sowing date, use of partially resistant chickpea genotypes, and seed and soil treatments with biocontrol agents Bacillus megaterium RGAF 51, B. subtilis GB03, nonpathogenic F. oxysporum Fo 90105, and Pseudomonas fluorescens RG 26. Advancing the sowing date from early spring to winter significantly delayed disease onset, reduced the final disease intensity (amount of disease in a microplot that combines disease incidence and severity, expressed as a percentage of the maximum possible amount of disease in that microplot), and increased chickpea seed yield. A significant linear relationship was found between disease development over time and weather variables at the experimental site, with epidemics developing earlier and faster as mean temperature increased and accumulated rainfall decreased. Under conditions highly conducive for Fusarium wilt development, the degree of disease control depended primarily on choice of sowing date, and to a lesser extent on level of resistance of chickpea genotypes to F. oxysporum f. sp. ciceris race 5, and the biocontrol treatments. The main effects of sowing date, partially resistant genotypes, and biocontrol agents were a reduction in the rate of epidemic development over time, a reduction of disease intensity, and an increase in chickpea seedling emergence, respectively. Chickpea seed yield was influenced by all three factors in the study. The increase in chickpea seed yield was the most consistent effect of the biocontrol agents. However, that effect was primarily influenced by sowing date, which also determined disease development. Effectiveness of biocontrol treatments in disease management was lowest in January sowings, which were least favorable for Fusarium wilt. Sowing in February, which was moderately favorable for wilt development, resulted in the greatest increase in seed yield by the biocontrol agents. In March sowings, which were most conducive for the disease, the biocontrol agents delayed disease onset and increased seedling emergence. B. subtilis GB03 and P. fluorescens RG 26, applied either alone or each in combination with nonpathogenic F. oxysporum Fo 90105, were the most effective treatments at suppressing Fusarium wilt, or delaying disease onset and increasing seed yield, respectively. The importance of integrating existing control practices, partially effective by themselves, with other control measures to achieve appropriate management of Fusarium wilt and increase of seed yield in chickpea in Mediterranean-type environments is demonstrated by the results of this study.

scholarly journals Efficacy of fungicides in controlling fusarium wilt of gladiolus

2018 ◽  
Vol 42 (4) ◽  
pp. 599-607
Author(s):  
L Yasmin ◽  
MA Ali ◽  
FN Khan
Keyword(s):  
Fusarium Oxysporum ◽  
Fusarium Wilt ◽  
Field Condition ◽  
Agricultural Research ◽  
Disease Incidence ◽  
Wilt Disease ◽  
Spike Length ◽  
Research Centre ◽  
Agricultural Research Institute ◽  
Infested Field

The efficacy of fungicides in controlling Fusarium wilt of gladiolus was studied at Horticulture Research Centre (HRC), Bangladesh Agricultural Research Institute (BARI), Gazipur during 2010-2012 following RCB design with four replications. Six fungicides such as Bavistin (0.1%), Provax (0.2%), Mancozeb (0.2%), Rovral (0.2%), Chlorax (10%) and Cupravit (0.7%) were evaluated against the Fusarium wilt disease of gladiolus (Fusarium oxysporum f. sp. gladioli) under naturally infested field condition. Bavistin was very effective in reducing the disease incidence and thereby resulting maximum corm germination (99.98%), spike length (73.90 cm), rachis length (43.70 cm), florets spike-1 (12.63), flower sticks plot-1 (38.75) and corm plot-1 (60.23) and cormel yield ha-1 (2.51 t) of gladiolus. Provax and Cupravit were also effective in inhibiting the disease incidence as well as better spike length, rachis length, florets spike-1, no of flower sticks, corm and cormel yield.Bangladesh J. Agril. Res. 42(4): 599-607, December 2017

scholarly journals Control of Fusarium Wilt of Watermelon by Grafting onto Bottlegourd or Interspecific Hybrid Squash Despite Colonization of Rootstocks by Fusarium

Plant Disease ◽  
2014 ◽  
Vol 98 (2) ◽  
pp. 255-266 ◽  
Author(s):  
A. P. Keinath ◽  
R. L. Hassell
Keyword(s):  
Fusarium Wilt ◽  
Interspecific Hybrid ◽  
Citrullus Lanatus ◽  
Disease Incidence ◽  
Field Performance ◽  
Control Treatment ◽  
Infested Field ◽  
Race 1 ◽  
Seedless Watermelon ◽  
Race 2

Grafting watermelon (Citrullus lanatus var. lanatus) onto rootstocks of interspecific hybrid squash (Cucurbita moschata × C. maxima), bottle gourd (Lagenaria siceraria), or citron (Citrullus lanatus var. citroides) has been used in Asia and Israel to mange Fusarium wilt of watermelon caused by Fusarium oxysporum f. sp. niveum. The objectives of this study were to determine the frequency of infection of six rootstocks by F. oxysporum f. sp. niveum races 1 and 2 and the field performance of grafted rootstocks in Charleston, SC. Grafted and nongrafted watermelon and rootstock plants were inoculated in the greenhouse with race 1, race 2, or water (the control treatment). With both races, the frequency of recovery of F. oxysporum from scion and rootstock portions of inoculated watermelon plants grafted onto ‘Ojakkyo’ citron was greater than from watermelon plants grafted onto ‘Shintosa Camel’ and ‘Strong Tosa’ interspecific hybrid squash, and from plants grafted onto ‘Emphasis’, ‘Macis’, and ‘WMXP 3945’ bottlegourd. For nongrafted plants inoculated with race 1, percent recovery also was greater from Ojakkyo than from interspecific hybrid squash and bottlegourd. For nongrafted plants inoculated with race 2, F. oxysporum was recovered from the base of ≥79% of all inoculated plants. More than two-thirds (15) of 21 isolates recovered from the tops or scions of inoculated plants were pathogenic on watermelon. In spring 2010 and 2011, the six rootstocks were grafted with seedless watermelon ‘Tri-X 313’, which is susceptible to both races, and transplanted in a field infested with races 1 and 2 of F. oxysporum f. sp. niveum. Disease incidence for nongrafted and self-grafted Tri-X 313 (the control treatments) and Tri-X 313 grafted onto Ojakkyo citron did not differ significantly. Grafted watermelon plants produced greater weights and numbers of fruit than plants of the two control treatments. Nonpathogenic isolates of F. oxysporum and isolates of F. oxysporum f. sp. niveum colonized interspecific hybrid squash, bottlegourd, and grafted watermelon. The rootstocks evaluated, however, restricted movement of F. oxysporum f. sp. niveum into the watermelon scion, suppressed wilt symptoms, and increased fruit yields in an infested field.

scholarly journals Influence of agro-climatic factors on the development of common soybean diseases

2020 ◽  
Vol 107 (3) ◽  
pp. 45-53
Author(s):  
G. D. Pospielova ◽  
◽  
N. P. Kovalenko ◽  
N. I. Nechyporenko ◽  
V. Ya. Kocherga ◽  
...  
Keyword(s):  
Fusarium Wilt ◽  
Environmental Conditions ◽  
Climatic Factors ◽  
Disease Prevalence ◽  
Disease Development ◽  
Soybean Diseases

Influence of agro-climatic factors on the development of common soybean diseases The article presents the results of the study of the influence of agro-climatic factors on the development of major soybean diseases (varieties Anthracite, Sprytna and Perlyna) during 2016-2019 in terms of commercial crops. The dominance of the pathogens Alternaria, Fusarium, Septoria and bacterial burns among the crops was determined. The connection between the development and spread of soybean diseases and hydrothermal environmental conditions was studied. Keywords: soybean, alternariosis, septoria, fusarium wilt, bacterial burns, disease development, disease prevalence.

Induction and resistance against Fusarium wilt disease of tomato by using sweet basil (Ocimum basilicum L) extract

2015 ◽  
Vol 95 (4) ◽  
pp. 689-701 ◽  
Author(s):  
Samia Ageeb Akladious ◽  
George Saad Isaac ◽  
Medhat Ahmed Abu-Tahon
Keyword(s):  
Fusarium Wilt ◽  
Growth Parameters ◽  
Disease Incidence ◽  
Ocimum Basilicum ◽  
Wilt Disease ◽  
Enzymatic Antioxidants ◽  
Sweet Basil ◽  
Fusarium Wilt Disease ◽  
Healthy Control

Akladious, S. A., Isaac, G. S. and Abu-Tahon, M. A. 2015. Induction and resistance against Fusarium wilt disease of tomato by using sweet basil (Ocimum basilicum L) extract. Can. J. Plant Sci. 95: 689–701. The antifungal activity of Ocimum basilicum (sweet basil) extract against Fusarium oxysporum f. sp. lycopersici race 3 the causal agent of tomato wilt and its ability in inducing disease resistance were studied in vivo using seed-soaking treatment before sowing. Plants were harvested at 45 and 105 d (vegetative and flowering stages) after sowing. Treatment with O. basilicum extract decreased the disease incidence from 94.70 to 18.00%. Results revealed that growth parameters and photosynthetic pigments were markedly inhibited in tomato plants in response to Fusarium wilt disease, whereas the contents of non-enzymatic and enzymatic antioxidants were increased as compared with healthy control plants. Moreover, presoaking in basil extract enhanced all the mentioned parameters in both healthy and infected plants. SDS-PAGE analysis of tomato leaves revealed that seed treated with basil extract resulted in an induction of novel protein bands during the vegetative stage. These new proteins were not detected in untreated healthy or infected control plants. Electrophoretic studies of polyphenol oxidase, esterase and malate dehydrogenase isoenzymes showed wide variations in their intensities and densities among all treatments. It seems that O. basilicum extract was able to enhance the biological control of Fusarium wilt disease of tomato.

PERSISTENCE AND PHYTOTOXICITY OF DINITROANILINE HERBICIDES IN MANITOBA SOILS

1980 ◽  
Vol 60 (1) ◽  
pp. 5-11 ◽  
Author(s):  
M. K. PRITCHARD ◽  
E. H. STOBBE
Keyword(s):  
Organic Matter ◽  
Environmental Conditions ◽  
Sandy Loam ◽  
Organic Matter Content ◽  
Matter Content ◽  
Soil Types ◽  
Clay Loam ◽  
Crop Injury ◽  
Dinitroaniline Herbicides

The persistence and phytotoxicity of dinitramine (n4, N4-diethyl-α,α,α-trifluoro-3,5-dinitrotoluene-2,4-diamine), fluchloralin (N-(2-chloroethyl)-2, 6-dinitro- N-propyl-4- (trifluoromethyl)aniline), profluralin (N-(cyclopropylmethyl)α,α,α-trifluoro-2, 6-dinitro-N-propyl-p-toluidine), and trifluralin (α,α,α-trifluoro-2,6-dinitro-N-N-dipropyl-p-toluidine) were compared in three Manitoba soils: sandy loam, clay loam and clay. The phytotoxicities of all chemicals decreased with increased organic matter. The phytotoxicities of the dinitroanilines were: dinitramine > trifluralin > profluralin = fluchloralin. Increased organic matter increased the persistence of trifluralin and fluchloralin. Profluralin and dinitramine persistence increased with increased clay and organic matter content. Profluralin was the most persistent chemical over all soil types. Under environmental conditions that retard dinitroaniline loss from Manitoba soils, residues of some of these herbicides may cause crop injury the year after application.

scholarly journals Degradation of atrazine and bromacil in two forestry waste products

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Trevor K. James ◽  
Hossein Ghanizadeh ◽  
Kerry C. Harrington ◽  
Nanthi S. Bolan
Keyword(s):  
Organic Matter ◽  
Organic Matter Content ◽  
Matter Content ◽  
Organic Media ◽  
Organic Carbon Content ◽  
Waste Products ◽  
Herbicide Degradation ◽  
Temperature Regimes ◽  
Degradation Rates ◽  
Subsequent Degradation

AbstractThe persistence and degradation of two common herbicides, atrazine and bromacil in two organic media, wood pulp and sawdust were compared with two soils. The hypothesis tested was that herbicide degradation will be faster in high organic matter media compared to soil. Degradation of two herbicides was carried out in four different temperature regimes and in sterilised media. The degradation half-life (t½) was determined under above-mentioned conditions then compared to degradation in soil. The degradation as quantified by t½ of the herbicides was generally longer in both organic media. Although microbial degradation was an important factor in the mineralisation of these herbicides, overall, the pH of the media had a more profound effect on the desorption and subsequent degradation rate than the organic carbon content. The results of this study revealed that the hypothesis was only partially correct as organic matter content per se did not strongly relate to degradation rates which were mainly governed by pH and microbial activity.

scholarly journals The Effects of Vermicompost on Yield and Some Growth Parameters of Lettuce

2017 ◽  
Vol 5 (12) ◽  
pp. 1566 ◽  
Author(s):  
Alper Durak ◽  
Özlem Altuntaş ◽  
İbrahim Kutalmış Kutsal ◽  
Rabia Işık ◽  
Fırat Ege Karaat
Keyword(s):  
Organic Matter ◽  
Soil Organic Matter ◽  
Production Systems ◽  
Growth Parameters ◽  
Farmyard Manure ◽  
Organic Matter Content ◽  
Soil Improvement ◽  
Matter Content ◽  
Biological Quality ◽  
Physical Chemical

Due to its importance for organic agriculture, one of the most important agricultural production systems, various sources have been proposed to increase soil organic matter content. Vermicompost is one of those sources known as the resistant last form of farmyard manure which is degraded by earthworms. Soil organic matter or humus and their compounds are nutrient sources that increase physical, chemical and biological quality of soil. Humus and humus compounds improve soil physical, chemical and biological quality parameters increasing rhizosphere aeration and water holding capacity, rehabilitating soil structure, providing plant nutrients and constituting natural media for microorganisms with their regulative effects on soil reactions. In this study, the effects of vermicompost on yield and some growth parameters of lettuce were investigated. For this purpose, a random block designed experiment with 5 different applications was conducted in 3 replicates. The applications were control, vermicompost applications of 100 kg, 200 kg and 300 kg per decare and conventional fertilization. According to the results obtained, yield and growth parameters were improved by vermicompost application when compared to control and conventional fertilization. As a result of this study, it was concluded that 300 kg vermicompost/da is a promising application in lettuce production for optimal yield and soil improvement.

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