Antimicrobial activities of Korean mugwort (Artemisia iwayomogi and Artemisia princeps) extracts against Staphylococcus aureus and Cutibacterium acnes

Research background. Antioxidants are described as important compounds that are present at low concentrations to inhibit oxidation processes. Due to the side effects of synthetic antioxidants, research interest has increased considerably towards finding natural sources of antioxidants that can replace synthetic antioxidants. The emergence and spread of antibiotic resistance require the development of new drugs or some potential sources of novel medicine. This work aims to extract the secondary metabolites of S. cerevisiae using ethyl acetate as a solvent and to determine the antioxidant and antimicrobial activities of these extracted metabolites. Experimental approach. The antioxidant activities of the secondary metabolites of S. cerevisiae were determined using DPPH, ABTS, and FRAP assays. Furthermore, the antimicrobial potential of the ethyl acetate extract of S. cerevisiae in treating Cutibacterium acnes, Staphylococcus aureus, and Staphylococcus epidermidis was assessed. Results and conclusion. Five out of 13 of the extracted secondary metabolites were identified as antioxidants. The antioxidant activity of the S. cerevisiae extract exhibited relatively high IC50 of 455.2689 μg/mL and 294.51 μg/mL for DPPH and ABTS respectively while the FRAP value was obtained as 44.4004 μg AAE/mL. Moreover, the extracts presented a significant antibacterial activity (p<0.05) against Staphylococcus aureus and Staphylococcus epidermidis at the concentrations of 100 mg/mL and 200 mg/mL, respectively. However, no inhibitory effect was observed against Cutibacterium acnes as the extract was only effective against Cutibacterium acnes at the concentrations of 300 mg/mL and 400 mg/mL (inhibition zones ranging from 9.0±0 to 9.333±0.577) respectively (p<0.05). Staphylococcus aureus was highly sensitive to the extract, with a MIC value of 18.75 mg/mL. Novelty and scientific contribution. This report confirmed the efficacy of the secondary metabolites of S. cerevisiae as a natural source of antioxidants and antimicrobials and suggest the possibility of employing them in drugs for the treatment of infectious diseases caused by the tested microorganisms.

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The Antimicrobial and Anti-Inflammatory Effects of Silver Nanoparticles Synthesised from Cotyledon orbiculata Aqueous Extract

Nanomaterials ◽

10.3390/nano11051343 ◽

2021 ◽

Vol 11 (5) ◽

pp. 1343

Author(s):

Caroline Tyavambiza ◽

Abdulrahman Mohammed Elbagory ◽

Abram Madimabe Madiehe ◽

Mervin Meyer ◽

Samantha Meyer

Keyword(s):

Staphylococcus Aureus ◽

Silver Nanoparticles ◽

Metallic Nanoparticles ◽

Methicillin Resistance ◽

Antimicrobial Activities ◽

Transmission Electron ◽

Anti Inflammatory ◽

Anti Inflammation ◽

Important Medicinal Plant ◽

Pro Inflammatory Cytokines

Cotyledon orbiculata, commonly known as pig’s ear, is an important medicinal plant of South Africa. It is used in traditional medicine to treat many ailments, including skin eruptions, abscesses, inflammation, boils and acne. Many plants have been used to synthesize metallic nanoparticles, particularly silver nanoparticles (AgNPs). However, the synthesis of AgNPs from C. orbiculata has never been reported before. The aim of this study was to synthesize AgNPs using C. orbiculata and evaluate their antimicrobial and immunomodulatory properties. AgNPs were synthesized and characterized using Ultraviolet-Visible Spectroscopy (UV-Vis), Dynamic Light Scattering (DLS) and High-Resolution Transmission Electron Microscopy (HR-TEM). The antimicrobial activities of the nanoparticles against skin pathogens (Staphylococcus aureus, Staphylococcus epidermidis, Methicillin Resistance Staphylococcus aureus, Pseudomonas aeruginosa and Candida albicans) as well as their effects on cytokine production in macrophages (differentiated from THP-1 cells) were evaluated. The AgNPs from C. orbiculata exhibited antimicrobial activity, with the highest activity observed against P. aeruginosa (5 µg/mL). The AgNPs also showed anti-inflammatory activity by inhibiting the secretion of pro-inflammatory cytokines (TNF-alpha, IL-6 and IL-1 beta) in lipopolysaccharide-treated macrophages. This concludes that the AgNPs produced from C. orbiculata possess antimicrobial and anti-inflammation properties.

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Antagonism between Aminoglycosides and β-Lactams in a Methicillin-Resistant Staphylococcus aureus Isolate Involves Induction of an Aminoglycoside-Modifying Enzyme

Antimicrobial Agents and Chemotherapy ◽

10.1128/aac.46.5.1516-1521.2002 ◽

2002 ◽

Vol 46 (5) ◽

pp. 1516-1521 ◽

Cited By ~ 7

Author(s):

Takashi Ida ◽

Ryoichi Okamoto ◽

Masato Nonoyama ◽

Kazuhiko Irinoda ◽

Mizuyo Kurazono ◽

...

Keyword(s):

Staphylococcus Aureus ◽

Methicillin Resistant Staphylococcus Aureus ◽

Antimicrobial Activities ◽

Hybrid Structure ◽

Conjugative Plasmid ◽

Pcr Analysis ◽

Aminoglycoside Resistance ◽

Gene Encoding ◽

Methicillin Resistant ◽

Aureus Isolate

ABSTRACT We encountered three clinical isolates of methicillin-resistant Staphylococcus aureus which were susceptible to netilmicin and arbekacin in the absence of β-lactam antibiotics but which were resistant to them in the presence of β-lactam antibiotics. One of these strains, KU5801, was used to further investigate the antagonism between aminoglycosides and β-lactam antibiotics. β-Lactam antibiotics induced bacterial synthesis of aminoglycoside-6′-N-acetyltransferase and 2"-O-phosphotransferase [AAC(6′)-APH(2")] in association with decreased antimicrobial activities of aminoglycosides. A 14.4-kb EcoRI fragment that included the genes that control for β-lactam-inducible aminoglycoside resistance was cloned from a 31-kb conjugative plasmid present in KU5801. Restriction fragment mapping and PCR analysis suggested that a Tn4001-like element containing a gene encoding AAC(6′)-APH(2") was located downstream from a truncated blaZ gene. The DNA sequence between blaR1 and a Tn4001-like element was determined. The Tn4001-IS257 hybrid structure was cointegrated into the blaZ gene, and the typical sequences for the termination of transcription were not found between these regions. We deduced that antagonism of aminoglycosides by β-lactam antibiotics in isolate KU5801 involved transcription of the aac(6′)-Ie-aph(2")-Ia gene under the influence of the system regulating penicillinase production.

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Synthesis and antimicrobial activities of novel n-substituted 8-(1-alkyl/alkylsulphonyl/alkoxycarbonyl-benzimidazol-2-ylmethoxy)-5-chloroquinolines

Journal of the Serbian Chemical Society ◽

10.2298/jsc100817105c ◽

2011 ◽

Vol 76 (9) ◽

pp. 1199-1206 ◽

Cited By ~ 8

Author(s):

Raju Chaudhari ◽

Sahebrao Rindhe

Keyword(s):

Staphylococcus Aureus ◽

Antibacterial Activity ◽

Nitrogen Heterocycles ◽

Antimicrobial Activities ◽

Mass Spectral Data ◽

Antifungal Activities ◽

Mass Spectral ◽

Electrophilic Reagents ◽

Almost All ◽

And Staphylococcus Aureus

Herein the synthesis of a series of novel 8-(1- alkyl/alkylsulphonyl/alkoxycarbonyl-benzimidazol-2-ylmethoxy)-5- chloroquinoline derivatives is reported. These derivatives were prepared by the condensation of o-phenylenediamine with [(5-chloroquinolin-8- yl)oxy]acetic acid, followed by substitution at nitrogen with different electrophilic reagents in presence of an appropriate base to give a series of nitrogen heterocycles containing the benzimidazole and quinoline nuclei. The structures of the compounds were confirmed based on 1H-NMR, 13CNMR, IR and mass spectral data. Almost all the compounds exhibited promising antibacterial activity against Salmonella typhimurium and Staphylococcus aureus. Some of the compounds showed good antifungal activities against Aspergillus niger but the antifungal activities against Candida albicans were disappointing.

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Highlighting the Biotechnological Potential of Deep Oceanic Crust Fungi through the Prism of Their Antimicrobial Activity

Marine Drugs ◽

10.3390/md19080411 ◽

2021 ◽

Vol 19 (8) ◽

pp. 411

Author(s):

Maxence Quemener ◽

Marie Dayras ◽

Nicolas Frotté ◽

Stella Debaets ◽

Christophe Le Meur ◽

...

Keyword(s):

Staphylococcus Aureus ◽

Antimicrobial Activity ◽

Oceanic Crust ◽

Gene Clusters ◽

Multidrug Resistant ◽

Antimicrobial Activities ◽

Antimicrobial Compounds ◽

Human Pathogens ◽

Biotechnological Potential ◽

Fungal Isolates

Among the different tools to address the antibiotic resistance crisis, bioprospecting in complex uncharted habitats to detect novel microorganisms putatively producing original antimicrobial compounds can definitely increase the current therapeutic arsenal of antibiotics. Fungi from numerous habitats have been widely screened for their ability to express specific biosynthetic gene clusters (BGCs) involved in the synthesis of antimicrobial compounds. Here, a collection of unique 75 deep oceanic crust fungi was screened to evaluate their biotechnological potential through the prism of their antimicrobial activity using a polyphasic approach. After a first genetic screening to detect specific BGCs, a second step consisted of an antimicrobial screening that tested the most promising isolates against 11 microbial targets. Here, 12 fungal isolates showed at least one antibacterial and/or antifungal activity (static or lytic) against human pathogens. This analysis also revealed that Staphylococcus aureus ATCC 25923 and Enterococcus faecalis CIP A 186 were the most impacted, followed by Pseudomonas aeruginosa ATCC 27853. A specific focus on three fungal isolates allowed us to detect interesting activity of crude extracts against multidrug-resistant Staphylococcus aureus. Finally, complementary mass spectrometry (MS)-based molecular networking analyses were performed to putatively assign the fungal metabolites and raise hypotheses to link them to the observed antimicrobial activities.

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Regulation of Monospecies and Mixed Biofilms Formation of Skin Staphylococcus aureus and Cutibacterium acnes by Human Natriuretic Peptides

Frontiers in Microbiology ◽

10.3389/fmicb.2018.02912 ◽

2018 ◽

Vol 9 ◽

Cited By ~ 8

Author(s):

Andrei Vladislavovich Gannesen ◽

Olivier Lesouhaitier ◽

Pierre-Jean Racine ◽

Magalie Barreau ◽

Alexander I. Netrusov ◽

...

Keyword(s):

Staphylococcus Aureus ◽

Natriuretic Peptides ◽

Cutibacterium Acnes

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Preparation of the Hybrids of Hydrotalcites and Chitosan by Urea Method and Their Antimicrobial Activities

Polymers ◽

10.3390/polym11101588 ◽

2019 ◽

Vol 11 (10) ◽

pp. 1588 ◽

Cited By ~ 4

Author(s):

Bi Foua Claude Alain Gohi ◽

Hong-Yan Zeng ◽

Xiao-Ju Cao ◽

Kai-Min Zou ◽

Wenlin Shuai ◽

...

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Surface Properties ◽

Antimicrobial Agents ◽

Materials Science ◽

Mechanical Stability ◽

Antimicrobial Activities ◽

Molar Ratio ◽

Penicillium Cyclopium ◽

Urea Method

Hybrid nano-supra molecular structured materials can boost the functionality of nano- or supra-molecular materials by providing increased reactivity and conductivity, or by simply improving their mechanical stability. Herein, the studies in materials science exploring hybrid systems are investigated from the perspective of two important related applications: healthcare and food safety. Interfacing phase strategy was applied, and ZnAl layered double hydroxide-chitosan hybrids, prepared by the urea method (U-LDH/CS), were successfully synthesized under the conditions of different chitosan(CS) concentrations with a Zn/Al molar ratio of 5.0. The structure and surface properties of the U-LDH/CS hybrids were characterized by X-ray diffraction (XRD), Fourier-transform infrared spectrometer(FTIR), scanning electronmicroscopy (SEM), ultravioletvisible (UV-Vis), and zero point charge (ZPC) techniques, where the effect of CS concentration on the structure and surface properties was investigated. The use of the U-LDH/CS hybrids as antimicrobial agents against Escherichia coli, Staphylococcus aureus, and Penicillium cyclopium was investigated in order to clarify the relationship between microstructure and antimicrobial ability. The hybrid prepared in a CS concentration of 1.0 g∙L−1 (U-LDH/CS1) exhibited the best antimicrobial activity and exhibited average inhibition zones of 24.2, 30.4, and 22.3mm against Escherichia coli, Staphylococcus aureus, and Penicillium cyclopium, respectively. The results showed that the appropriate addition of CS molecules could increase antimicrobial ability against microorganisms.

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Synthesis and investigation of 3,5-bis-linear and macrocyclic tripeptidopyridine candidates by using l-valine, N,N′-(3,5-pyridinediyldicarbonyl)bis-dimethyl ester as synthon

Zeitschrift für Naturforschung B ◽

10.1515/znb-2019-0006 ◽

2019 ◽

Vol 74 (6) ◽

pp. 473-478 ◽

Cited By ~ 2

Author(s):

Abd El-Galil E. Amr ◽

Ahmed M. Naglah ◽

Nermien M. Sabry ◽

Alhussein A. Ibrahim ◽

Elsayed A. Elsayed ◽

...

Keyword(s):

Staphylococcus Aureus ◽

Candida Albicans ◽

Organic Molecules ◽

Biological Activities ◽

Dimethyl Ester ◽

Antimicrobial Activities ◽

Current Work ◽

Antifungal Activities ◽

Antibacterial And Antifungal Activities ◽

Antibacterial And Antifungal

AbstractInterest in the synthesis of heterocyclic organic molecules with peptide moieties has gained attention due to their potential biological activities. The current work aimed at synthesizing new macrocyclic tripeptide imides and evaluating their possible antimicrobial activities. A series of 11 derivatives were prepared from dimethyl 3,5-pyridinevalinyl ester either by NaOH or NH2NH2 treatment, followed by cyclization and further reaction with NaOH or NH2NH2. The majority of synthesized derivatives showed promising antibacterial and antifungal activities in comparison to standard known antibiotics. Compounds 5a and 7b showed the most potential antibacterial against Staphylococcus aureus and antifungal activities against Candida albicans, respectively.

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Inhibitory Spectra and Modes of Antimicrobial Action of Gallotannins from Mango Kernels (Mangifera indicaL.)

Applied and Environmental Microbiology ◽

10.1128/aem.02521-10 ◽

2011 ◽

Vol 77 (7) ◽

pp. 2215-2223 ◽

Cited By ~ 62

Author(s):

Christina Engels ◽

Andreas Schieber ◽

Michael G. Gänzle

Keyword(s):

Staphylococcus Aureus ◽

Wild Type Strain ◽

Antimicrobial Activities ◽

Resistance Mechanisms ◽

Gram Negative Bacteria ◽

Wild Type ◽

Gram Positive Bacteria ◽

Food Borne ◽

Membrane Bound ◽

Bacteria And Fungi

ABSTRACTThis study investigated the antimicrobial activities and modes of action of penta-, hexa-, hepta-, octa-, nona-, and deca-O-galloylglucose (gallotannins) isolated from mango kernels. The MICs and minimum bactericidal concentrations (MBCs) against food-borne bacteria and fungi were determined using a critical dilution assay. Gram-positive bacteria were generally more susceptible to gallotannins than were Gram-negative bacteria. The MICs of gallotannins againstBacillus subtilis,Bacillus cereus,Clostridium botulinum,Campylobacter jejuni,Listeria monocytogenes, andStaphylococcus aureuswere 0.2 g liter−1or less; enterotoxigenicEscherichia coliandSalmonella entericawere inhibited by 0.5 to 1 g liter−1, and lactic acid bacteria were resistant. The use of lipopolysaccharide mutants ofS. entericaindicated that the outer membrane confers resistance toward gallotannins. Supplementation of LB medium with iron eliminated the inhibitory activity of gallotannins againstStaphylococcus aureus, and siderophore-deficient mutants ofS. entericawere less resistant toward gallotannins than was the wild-type strain. Hepta-O-galloylglucose sensitizedLactobacillus plantarumTMW1.460 to hop extract, indicating inactivation of hop resistance mechanisms, e.g., the multidrug resistance (MDR) transporter HorA. Carbohydrate metabolism ofLactococcus lactisMG1363, a conditionally respiring organism, was influenced by hepta-O-galloylglucose when grown under aerobic conditions and in the presence of heme but not under anaerobic conditions, indicating that gallotannins influence the respiratory chain. In conclusion, the inhibitory activities of gallotannins are attributable to their strong affinity for iron and likely additionally relate to the inactivation of membrane-bound proteins.

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Which Approach Is More Effective in the Selection of Plants with Antimicrobial Activity?

Evidence-based Complementary and Alternative Medicine ◽

10.1155/2013/308980 ◽

2013 ◽

Vol 2013 ◽

pp. 1-9 ◽

Cited By ~ 15

Author(s):

Ana Carolina Oliveira Silva ◽

Elidiane Fonseca Santana ◽

Antonio Marcos Saraiva ◽

Felipe Neves Coutinho ◽

Ricardo Henrique Acre Castro ◽

...

Keyword(s):

Staphylococcus Aureus ◽

Antimicrobial Activity ◽

Minimum Inhibitory Concentration ◽

Crude Extract ◽

Inhibitory Concentration ◽

Antimicrobial Activities ◽

Semiarid Region ◽

Northeast Brazil ◽

Plant Drugs ◽

Selection Of

The development of the present study was based on selections using random, direct ethnopharmacological, and indirect ethnopharmacological approaches, aiming to evaluate which method is the best for bioprospecting new antimicrobial plant drugs. A crude extract of 53 species of herbaceous plants collected in the semiarid region of Northeast Brazil was tested against 11 microorganisms. Well-agar diffusion and minimum inhibitory concentration (MIC) techniques were used. Ten extracts from direct, six from random, and three from indirect ethnopharmacological selections exhibited activities that ranged from weak to very active against the organisms tested. The strain most susceptible to the evaluated extracts wasStaphylococcus aureus. The MIC analysis revealed the best result for the direct ethnopharmacological approach, considering that some species yielded extracts classified as active or moderately active (MICs between 250 and 1000 µg/mL). Furthermore, one species from this approach inhibited the growth of the threeCandidastrains. Thus, it was concluded that the direct ethnopharmacological approach is the most effective when selecting species for bioprospecting new plant drugs with antimicrobial activities.

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