scholarly journals Impact of formate supplementation on body weight and plasma amino acids

2020 ◽  
Author(s):  
Sandeep Dhayade ◽  
Matthias Pietzke ◽  
Robert Wiesheu ◽  
Jacqueline Tait-Mulder ◽  
Dimitris Athineos ◽  
...  
Keyword(s):  
Amino Acids ◽  
Body Weight ◽  
Immune Cell ◽  
Sodium Formate ◽  
The Body ◽  
Control Group ◽  
Male Mice ◽  
Female Mice ◽  
Cell Counts ◽  
The Impact

AbstractCurrent nutritional recommendations are focused on energy, fat, carbohydrate, protein and vitamins. Less attention has been paid to the nutritional demand of one-carbon units for nucleotide and methionine synthesis. Here we investigate the impact of sodium formate supplementation as a nutritional intervention to increase the dietary intake of one-carbon units. A cohort of six female and six male mice received 125 mM sodium formate in the drinking water for three months. A control group of another six female and six male mice was also followed up for the same period of time. Tail vein blood samples were collected once a month and profiled with a haematology Analyser. At the end of the study blood and tissues were collected for metabolomics analysis and immune cell sorting. Formate supplementation has no significant physiological effect on male mice. Formate supplementation has no significant effect on the immune cell counts during the intervention or at the end of the study in either gender. In female mice however, the body weight and spleen wet-weight were significantly increased by formate supplementation, while the blood plasma levels of amino acids were decreased. Formate supplementation also increased the frequency of probiotic bacteria in the stools of female mice. We conclude that formate supplementation induces physiological changes in female mice.

scholarly journals Impact of Formate Supplementation on Body Weight and Plasma Amino Acids

Nutrients ◽  
2020 ◽  
Vol 12 (8) ◽  
pp. 2181
Author(s):  
Sandeep Dhayade ◽  
Matthias Pietzke ◽  
Robert Wiesheu ◽  
Jacqueline Tait-Mulder ◽  
Dimitris Athineos ◽  
...  
Keyword(s):  
Amino Acids ◽  
Body Weight ◽  
Immune Cell ◽  
Sodium Formate ◽  
The Body ◽  
Control Group ◽  
Male Mice ◽  
Female Mice ◽  
Cell Counts ◽  
The Impact

Current nutritional recommendations are focused on energy, fat, carbohydrate, protein and vitamins. Less attention has been paid to the nutritional demand of one-carbon units for nucleotide and methionine synthesis. Here, we investigated the impact of sodium formate supplementation as a nutritional intervention to increase the dietary intake of one-carbon units. A cohort of six female and six male mice received 125 mM of sodium formate in the drinking water for three months. A control group of another six female and six male mice was also followed up for the same period of time. Tail vein blood samples were collected once a month and profiled with a haematology analyser. At the end of the study, blood and tissues were collected for metabolomics analysis and immune cell profiling. Formate supplementation had no significant physiological effect on male mice, except for a small decrease in body weight. Formate supplementation had no significant effect on the immune cell counts during the intervention or at the end of the study in either gender. In female mice, however, the body weight and spleen wet weight were significantly increased by formate supplementation, while the blood plasma levels of amino acids were decreased. Formate supplementation also increased the frequency of bifidobacteria, a probiotic bacterium, in the stools of female mice. We conclude that formate supplementation induces physiological changes in a gender-specific manner.

scholarly journals The Impact of Time-Restricted Feeding on Exercise-Induced Immune Parameters in C57BL/6 Male Mice

2020 ◽  
Vol 4 (Supplement_2) ◽  
pp. 1541-1541
Author(s):  
Marie van der Merwe ◽  
Martina Faietti ◽  
Richard Bloomer ◽  
Melissa Puppa ◽  
Aaron Persinger ◽  
...  
Keyword(s):  
Immune Cell ◽  
Control Group ◽  
Intermittent Fasting ◽  
Restricted Feeding ◽  
Male Mice ◽  
Immune Parameters ◽  
Tnf Α ◽  
Exercise Induced ◽  
Access To Food ◽  
The Impact

Abstract Objectives Food intake and exercise are considered modulators of the immune system. Specifically, intermittent fasting protocols have been demonstrated to reduce inflammation and alter cytokine responses. The objective of the current study was to determine if a form of intermittent fasting known as time-restricted feeding (TRF) would alter immune parameters in response to exercise. Methods 8-week-old C57BL/6 male mice were divided into three groups based on feeding schedule; group one had access to food ad libitum (Control) and groups two and three had access to food in a time restricted manner. Access was allowed for six hours per day either immediately after running (TRF-imm) or six hours after running (TRF-del). Mice ran on a treadmill for 1 hour, 5 days per week for eight weeks. Diet consisted of 21% protein, 16% fat and 64% carbohydrate. Weight, glucose and ketone levels, and immune populations were analyzed. Systemic IL-6 and TNF-α levels were measured before and after running. In a subpopulation, cytokine response to lipopolysaccharides (LPS) was also monitored. Results All mice gained weight during the eight-week intervention, but TRF-imm gained significantly less weight than Control (P = 0.02). No differences were detected in glucose levels. The ketone body β-hydroxybutyrate (BHB) was significantly higher at week eight in TRF groups (P ≤ 0.03) but running induced BHB in all groups to approximately 1 mM. Running reduced the blood lymphocytes levels (P < 0.05), with a concomitant increase of granulocytes (P < 0.05) in all groups. There was a small increase in monocytes only in the Control group (P = 0.017). No differences were detected in splenic immune populations, including CD4 and CD8 T cells, and CD11b + cells. Both IL-6 and TNF-α levels were low in all groups before exercise; however, post exercise IL-6 was increased, but not to the same extend in all groups. The IL-6 response was blunted in the TRF groups. The reduced levels of IL-6 was not due to loss of immune function, as both IL-6 and TNF-α were readily induced by exposure of mice to LPS. Conclusions Time-restricted feeding protocols did not induce differences in immune cell composition in blood or spleen but resulted in attenuated exercise-induced IL-6 levels. Funding Sources University of Memphis, School of Health Studies.

scholarly journals The impact of cognitive restructuring and self-compassion strategies on negative body image among women with higher body weight: an experimental investigation

2021 ◽  
Author(s):  
Lauren Alysha David
Keyword(s):  
Body Image ◽  
Body Weight ◽  
Body Dissatisfaction ◽  
Weight Status ◽  
Cognitive Restructuring ◽  
The Body ◽  
Control Group ◽  
Negative Body Image ◽  
Self Compassion ◽  
The Impact

Individuals with higher body weight are at a greater risk of having negative body image (Friedman & Brownell, 1995). Yet current body image interventions, such as Cognitive Behavioural Therapy (CBT), are largely tested with individuals with normal weight or individuals with eating disorders. Furthermore, cognitive restructuring, one of the key components of CBT for body image (Alleva et al., 2015), relies on the assumption that negative cognitions or appraisals regarding the body are unbalanced or distorted in some way. However, people with higher body weight are 50% more likely to experience major discrimination based on their weight status and thus may possess some “evidence” from lived experience of weight bias that would lend support to their negative body-related thoughts (Puhl & Brownell, 2001; 2006). The use of compassion-focused approaches might be particularly helpful in overcoming these obstacles. Self-compassion refers to the capacity for mindfully reflecting on one’s own perceived flaws, mistakes, or wrongdoings with kindness and with an appreciation for the inherent imperfection in everyone (Neff, 2013). The present study tested the impact of various thinking strategies for managing negative body image in women with higher body weight after getting on the scale, a commonly distressing body image trigger (Ogden & Evans, 1996). Participants (N = 79) were recruited from the community and screened for moderate body dissatisfaction. They were randomly assigned to receive a single training session in cognitive restructuring (CR), self-compassion (SC), or distraction (Control) strategies after being weighed. Participants in all three of the groups reported improvements in body dissatisfaction and negative affect immediately following the training. Relative to those in the Control group, those participants who received training in CR or SC strategies reported greater improvements in body image, body image flexibility, self-compassion, and cognitive distortions one week after the training. These findings suggest that CR and SC strategies may be helpful in improving the distress associated with being weighed among women with higher body weight. The results may have broader implications for the development of psychosocial interventions focused on improving body image among these individuals.

scholarly journals Schistosoma mansoni Infection Is Impacted by Malnutrition

2021 ◽  
Vol 12 ◽  
Author(s):  
Poliane Silva Maciel ◽  
Ricardo Gonçalves ◽  
Lis Ribeiro do Valle Antonelli ◽  
Cristina Toscano Fonseca
Keyword(s):  
Body Weight ◽  
Neglected Tropical Diseases ◽  
The Body ◽  
Control Group ◽  
Low Fat ◽  
Immunological Parameters ◽  
Low Protein ◽  
Cytokine Milieu ◽  
Complex Relationships ◽  
The Impact

Schistosomiasis remains one of the most important neglected tropical diseases in the world. It mainly affects developing countries, where it often coexists with malnutrition. Despite this, few studies have investigated the relationship between schistosomiasis and malnutrition. Herein, we evaluate the impact of malnutrition on experimental S. mansoni infection. Mice were divided into 5 groups: Control (Ctrl) diet (14% protein and 10% lipids), low-protein 3% (LP 3%), low-protein 8% (LP 8%), low-fat 2.5% (LF 2.5%), and low-fat 5% (LF 5%). Mice were fed with their respective diets and were infected when a difference of approximately 20% in the body weight between mice from any experimental group and mice from the control group was achieved. Nutritional, parasitological, and immunological parameters were assessed either just before infection and/or approximately 50 days later before mice were perfused. Our results showed that the 3% low-protein diet was the only one capable of establishing malnutrition in mice. Mice fed with this diet showed: (i) significant reduction in body weight and serum albumin levels before infection, (ii) decreased levels of all biochemical parameters evaluated before perfusion, (iii) decreased numbers of schistosome eggs trapped in intestines and impaired parasite fecundity, (iv) a delay in the granuloma development with a smaller granuloma area, and (v) reduced levels of IL-4 and IFN-γ in the liver. Our findings demonstrate that low protein supply leads to malnutrition in mice and impacts the cytokine milieu in the liver and granuloma formation. Additionally, the establishment of our murine malnutrition model will enable future studies aiming to better understand the complex relationships between nutrition, immune responses, and infection outcome.

Study the effect of alcoholic extractrawsalvia officnalis on some sex hormonesin female mice white

2018 ◽  
Vol 9 (SPL1) ◽  
Author(s):  
Frah razzaq Kbyeh
Keyword(s):  
Body Weight ◽  
Luteinizing Hormone ◽  
Follicle Stimulating Hormone ◽  
Physiological Solution ◽  
Control Group ◽  
Alcoholic Extract ◽  
Female Mice ◽  
Group B ◽  
The Impact ◽  
Stimulating Hormone

 Thirty female mice white used in the experiment for the purpose of knowing the effect of alcoholic extract raw salvia officnalisleaves on the level of sex hormones which were divided animals into three groups experimental rate of ten mice per group dosage group B and C oral daily dose of the extract amount 500 mg / kg of body weight the first group, respectively,in the1000 mg/ Kg of body weight,respectively,either the first set group A treated the same way time and himself as 0.1 ml / g body weight of physiological solution compared to the mind set lasted 30 days dosing was weight when you stop recording the dosage and then kill her blood was collected through a stab heart for the purpose of studies of hormonal (estrogen,progesterone,luteinizing hormone,follicle stimulating hormone and prolactin)extract reflected the impact of the variables studied and related to fertility in the following results compared with the control group. Low body weight was in the doses and the effect of the dose of 1000 mg / kg of body weight was significantly larger dose of 500 mg / kg of body weight at a level significantly 5% P0≤0.05. Significant increase in the level of hormones (progesterone,estrogen,luteinizing hormone,follicle-stimulating hormone) in the doses and the effect of the dose of1000 mg / kg of body weight was significantly larger dose of 500 mg / kg of body weight at a level significantly 5% P0≤0.05. Significant decrease in the level of the hormone prolactin in the doses and the effect of the dose of 1000 mg / kg of body weight was significantly larger dose of 500 mg / kg of body weight at a level significantly 5% P0≤0.05

L-Ascorbic Acid Addition to Chitosan Reduces Body Weight in Overweight Women

2014 ◽  
Vol 84 (1-2) ◽  
pp. 5-11 ◽  
Author(s):  
Eun Y. Jung ◽  
Sung C. Jun ◽  
Un J. Chang ◽  
Hyung J. Suh
Keyword(s):  
Ascorbic Acid ◽  
Body Weight ◽  
Fat Body ◽  
Body Weight Gain ◽  
Skinfold Thickness ◽  
The Body ◽  
Control Group ◽  
Percentage Body Fat ◽  
Overweight Women ◽  
Body Weights

Previously, we have found that the addition of L-ascorbic acid to chitosan enhanced the reduction in body weight gain in guinea pigs fed a high-fat diet. We hypothesized that the addition of L-ascorbic acid to chitosan would accelerate the reduction of body weight in humans, similar to the animal model. Overweight subjects administered chitosan with or without L-ascorbic acid for 8 weeks, were assigned to three groups: Control group (N = 26, placebo, vehicle only), Chito group (N = 27, 3 g/day chitosan), and Chito-vita group (N = 27, 3 g/day chitosan plus 2 g/day L-ascorbic acid). The body weights and body mass index (BMI) of the Chito and Chito-vita groups decreased significantly (p < 0.05) compared to the Control group. The BMI of the Chito-vita group decreased significantly compared to the Chito group (Chito: -1.0 kg/m2 vs. Chito-vita: -1.6 kg/m2, p < 0.05). The results showed that the chitosan enhanced reduction of body weight and BMI was accentuated by the addition of L-ascorbic acid. The fat mass, percentage body fat, body circumference, and skinfold thickness in the Chito and Chito-vita groups decreased more than the Control group; however, these parameters were not significantly different between the three groups. Chitosan combined with L-ascorbic acid may be useful for controlling body weight.

Influence of DAFS-25+polizon composition on blood and functional state of liver of steers at fattening

2020 ◽  
pp. 15-18
Author(s):  
Inna R. Kilmetova ◽  
◽  
Igor A. Rodin ◽  
Nazira I. Khayrullina ◽  
Nikolay G. Fenchenko ◽  
...  
Keyword(s):  
Functional State ◽  
White Blood Cells ◽  
Live Weight ◽  
The Body ◽  
Farm Animals ◽  
Control Group ◽  
Standard Diet ◽  
The Impact ◽  
Experimental Group ◽  
Stimulation Of

Summary. The disbalanced feeding and the uneven distribution of micro- and macroelements in the environment leads to a trace element, in particular hypomelanosis. To accelerate the growth and preservation of young farm animals include in the diet of various biological additives and drugs, which include selenium. For stimulation of weight gain in the livestock industry, as well as for the prevention and treatment of pathological processes in addition to micro - and macrouse amino acids, primarily methionine. The aim of this work was to study the influence of composition of DAFS-25+Polizon on morpho-biochemical parameters of blood and functional state of the liver in fattening bulls of black-motley breed in the conditions of the Republic of Bashkortostan. Experiments using were conducted on bull-calves of black-motley breed of the properties in the properties age from 6 to 15 months. The first experimental group during the experiment was additionally given the composition of DAFS-25+Polizon at a dose of 2 mg/kg, the animals of the control group received a standard diet. To assess the impact of the composition DAFS-25+Polizon on metabolism cattle studied morphological and biochemical indicators of blood and conducted histological examination of the liver. It is established that the use of the composition of DAFS-25+Polizon at a dose of 2 mg/kg increases the number of erythrocytes and hemoglobin in the experimental group and reduces the amount of white blood cells. The serum content of total protein, phosphorus and calcium increases in the group of experimental animals. Microscopic examination of the liver revealed no changes in the structure of the organ and hepatocytes in the experimental group, whereas in the control group hemodynamic disorders and dystrophic changes in liver cells were observed. Thus, the use of the composition DAFS-25+Polizon at a dose of 2 mg/kg of live weight in fattening bulls black-and-white breed contributes to the increase of redox processes in the body, stimulation of metabolism, prevent the development of liver disorders of cellular mechanisms of metabolism, optimizes the structure of the liver, which generally provides higher productivity.

The Effect of Feeding Date Palm Fruit (Phoeix dactylifera L.) on Energy, Macronutrient, and Fiber Intakes of a Convenient Sample of Females

2020 ◽  
Vol 16 (8) ◽  
pp. 1270-1277
Author(s):  
Hiba F. Al-Sayyed ◽  
Hamed R. Takruri ◽  
Nawal A. Bakir ◽  
Dima H. Takruri
Keyword(s):  
Body Weight ◽  
Body Fat ◽  
Date Palm ◽  
The Body ◽  
Control Group ◽  
Hormone Concentration ◽  
Palm Fruit ◽  
Menstrual Status ◽  
Feeding Duration ◽  
Effect Of Feeding

Background : Female menstrual changes in hormone concentration influence appetite and eating behavior. As well, eating pattern has some influences on menstrual hormonal status. Feeding date palm fruit has been found in our previous report to affect menstrual hormones and some menstrual parameters. Objective: The aim of this research is to study the effect of feeding dates on energy, macronutrient, and fiber intakes, body weight, and body fat%. Additionally, this study aims to find any correlation between menstrual hormone concentration and the above-mentioned parameters. Methods: A convenient sample (n=37) of not-sexually active females aged 20-30 were divided into two groups; one group (dates group) was fed 7 dates and the other served as a control group. The participants of the control group were exposed to the same experimental conditions except for eating dates. Throughout the feeding trial, the volunteers filled a 3-day food record; one menses day and two non-menses days. Results: There was a significant effect of the menstrual status-feeding duration interaction in terms of protein and fat intakes. Additionally, menstrual status, as well as feeding duration, affected energy and macronutrient intakes significantly. Furthermore, feeding duration affected fiber intake significantly. Despite the above-mentioned differences in energy and macronutrient intakes, these effects didn’t affect the body weight and body mass index of the study participants, neither their body fat%. Moreover, we have found significant correlations between nutrient intakes and menstrual hormone concentration. Conclusion : Feeding dates affected energy, macronutrient, and fiber intakes significantly. These effects were related to the changes in menstrual hormone concentration.

Effect of High Plane of Nutrition on Blood Biochemical Profile and Onset of Puberty in Prepubertal HF X Kankrej Crossbred Heifers

2019 ◽  
Vol 15 (02) ◽  
pp. 14-17
Author(s):  
K K Hadiya ◽  
A J Dhami ◽  
D V Chaudhari ◽  
P M Lunagariya
Keyword(s):  
Body Weight ◽  
Treatment Group ◽  
Plasma Levels ◽  
Age Of Onset ◽  
The Body ◽  
Monthly Interval ◽  
Control Group ◽  
Blood Biochemical ◽  
Lower Protein ◽  
The Mean

This study was initiated on 24 prepubertal Holstein x Kankrej crossbred heifers of nearly identical age (7-9 months) and body weight (130-140 kg) at University farm to evaluate the effect of high plane of nutrition on blood biochemical and minerals profile and the age at puberty. Twelve heifers were managed under routine farm feeding (control) and the rest 12 under ideal optimum feeding regime (treatment) that included extra 1 kg concentrate, 30 g min mix and ad-lib dry fodder. The body weight and ovarian ultrasonography together with blood sampling was carried out at monthly interval from 10 to 18 months of age to study the ovarian dynamics and blood biochemical changes. High plane of nutrition to growing heifers was beneficial in reducing the age of onset of puberty (by 2-3 months) compared to routine farm fed group. The mean plasma total protein and cholesterol concentrations showed a rising trend with significant variations from 10 to 16 months of age, where it got mostly stabilized indicating adult profile. The activity of enzymes GOT and GPT also rose gradually and significantly from 10 months till 14-15 months of age, and thereafter it remained more or less static till 18 months of age. The levels of both these enzymes were higher, with lower protein and cholesterol, in control than the treatment group from 15-16 months of age onwards. The mean plasma levels of both calcium and phosphorus increased gradually and significantly with advancing age till 16-17 months of age, with little higher values in supplemented than a control group. The plasma levels of zinc, iron, copper, and cobalt also showed rising trend with significant differences between 10th and 12th-14th months of age, and from 15th to 18th months of age the levels were statistically the same in all the groups with slightly higher values in the treatment group.

scholarly journals Melatonin and diet-induced metabolic syndrome in rats: impact on the hypophysial-testicular axis

2013 ◽  
Vol 16 (2) ◽  
Author(s):  
Pablo A. Scacchi Bernasconi ◽  
Nancy P. Cardoso ◽  
Roxana Reynoso ◽  
Pablo Scacchi ◽  
Daniel P. Cardinali
Keyword(s):  
Metabolic Syndrome ◽  
Body Weight ◽  
Uric Acid ◽  
Plasma Levels ◽  
Density Lipoprotein ◽  
The Body ◽  
High Fat ◽  
Testosterone Secretion ◽  
Diet Manipulation ◽  
The Impact

AbstractCombinations of fructose- and fat-rich diets in experimental animals can model the human metabolic syndrome (MS). In rats, the increase in blood pressure (BP) after diet manipulation is sex related and highly dependent on testosterone secretion. However, the extent of the impact of diet on rodent hypophysial-testicular axis remains undefined. In the present study, rats drinking a 10% fructose solution or fed a high-fat (35%) diet for 10 weeks had higher plasma levels of luteinizing hormone (LH) and lower plasma levels of testosterone, without significant changes in circulating follicle-stimulating hormone or the weight of most reproductive organs. Diet manipulation brought about a significant increase in body weight, systolic BP, area under the curve (AUC) of glycemia after an intraperitoneal glucose tolerance test (IPGTT), and plasma low-density lipoprotein cholesterol, cholesterol, triglycerides, and uric acid levels. The concomitant administration of melatonin (25 μg/mL of drinking water) normalized the abnormally high LH levels but did not affect the inhibited testosterone secretion found in fructose- or high-fat-fed rats. Rather, melatonin per se inhibited testosterone secretion. Melatonin significantly blunted the body weight and systolic BP increase, the increase in the AUC of glycemia after an IPGTT, and the changes in circulating lipid profile and uric acid found in both MS models. The results are compatible with a primary inhibition of testicular function in diet-induced MS in rats and with the partial effectiveness of melatonin to counteract the metabolic but not the testicular sequelae of rodent MS.

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