scholarly journals Twists and turns of the salicylate catabolism in Aspergillus terreus

2020 ◽  
Author(s):  
Tiago M. Martins ◽  
Celso Martins ◽  
Cristina Silva Pereira
Keyword(s):  
Organic Acids ◽  
Secondary Metabolism ◽  
Aromatic Hydrocarbons ◽  
Aromatic Compounds ◽  
Aspergillus Terreus ◽  
Raw Materials ◽  
Pharmaceutical Drugs ◽  
Cell Factories ◽  
New Genes ◽  
First Time

ABSTRACTIn fungi, salicylate catabolism was believed to proceed only through the catechol branch of the 3-oxoadipate pathway, as shown e.g. in Aspergillus nidulans. However, the observation of a transient accumulation of gentisate upon cultivation of Aspergillus terreus in salicylate media questions this concept. To address this we have run a comparative analysis of the transcriptome of these two species after growth in salicylate using acetate as a control condition. The results revealed the high complexity of the salicylate metabolism in A. terreus with the concomitant positive regulation of several pathways for the catabolism of aromatic compounds. This included the unexpected joint action of two pathways: the nicotinate and the 3-hydroxyanthranilate, possibly crucial for the catabolism of aromatics in this fungus. New genes participating in the nicotinate metabolism are here proposed, whereas the 3-hydroxyanthranilate catabolic pathway in fungi is described for the first time. The transcriptome analysis showed also for the two species an intimate relationship between salicylate catabolism and secondary metabolism. This study emphasizes that the central pathways for the catabolism of aromatic hydrocarbons in fungi hold many mysteries yet to be discovered.IMPORTANCEAspergilli are versatile cell factories used in industry for production of organic acids, enzymes and pharmaceutical drugs. To date, organic acids bio-based production relies on food substrates. These processes are currently being challenged to switch to renewable non-food raw materials; a reality that should inspire the use of lignin derived aromatic monomers. In this context, Aspergilli emerge at the forefront of future bio-based approaches due to their industrial relevance and recognized prolific catabolism of aromatic compounds. Notwithstanding considerable advances in the field, there are still important knowledge gaps in the central catabolism of aromatic hydrocarbons in fungi. Here, we disclosed a novel central pathway, defying previous established ideas on the central metabolism of the aromatic amino acid tryptophan in Ascomycota. We also observed that the catabolism of the aromatic salicylate greatly activated the secondary metabolism, furthering the significance of using lignin derived aromatic hydrocarbons as a distinctive biomass source.

scholarly journals Twists and Turns in the Salicylate Catabolism of Aspergillus terreus, Revealing New Roles of the 3-Hydroxyanthranilate Pathway

mSystems ◽  
2021 ◽  
Vol 6 (1) ◽  
Author(s):  
Tiago M. Martins ◽  
Celso Martins ◽  
Paula Guedes ◽  
Cristina Silva Pereira
Keyword(s):  
Organic Acids ◽  
Secondary Metabolism ◽  
Aromatic Hydrocarbons ◽  
Aromatic Compounds ◽  
Aspergillus Terreus ◽  
Raw Materials ◽  
Pharmaceutical Drugs ◽  
Content Type ◽  
Cell Factories ◽  
New Genes

ABSTRACT In fungi, salicylate catabolism was believed to proceed only through the catechol branch of the 3-oxoadipate pathway, as shown, e.g., in Aspergillus nidulans. However, the observation of a transient accumulation of gentisate upon the cultivation of Aspergillus terreus in salicylate medium questions this concept. To address this, we have run a comparative analysis of the transcriptome of these two species after growth in salicylate using acetate as a control condition. The results revealed the high complexity of the salicylate metabolism in A. terreus with the concomitant positive regulation of several pathways for the catabolism of aromatic compounds. This included the unexpected joint action of two pathways—3-hydroxyanthranilate and nicotinate—possibly crucial for the catabolism of aromatics in this fungus. Importantly, the 3-hydroxyanthranilate catabolic pathway in fungi is described here for the first time, whereas new genes participating in the nicotinate metabolism are also proposed. The transcriptome analysis showed also for the two species an intimate relationship between salicylate catabolism and secondary metabolism. This study emphasizes that the central pathways for the catabolism of aromatic hydrocarbons in fungi hold many mysteries yet to be discovered. IMPORTANCE Aspergilli are versatile cell factories used in industry for the production of organic acids, enzymes, and pharmaceutical drugs. To date, bio-based production of organic acids relies on food substrates. These processes are currently being challenged to switch to renewable nonfood raw materials—a reality that should inspire the use of lignin-derived aromatic monomers. In this context, aspergilli emerge at the forefront of future bio-based approaches due to their industrial relevance and recognized prolific catabolism of aromatic compounds. Notwithstanding considerable advances in the field, there are still important knowledge gaps in the central catabolism of aromatic hydrocarbons in fungi. Here, we disclose a novel central pathway, 3-hydroxyanthranilate, defying previously established ideas on the central metabolism of the aromatic amino acid tryptophan in Ascomycota. We also observe that the catabolism of the aromatic salicylate greatly activated the secondary metabolism, furthering the significance of using lignin-derived aromatic hydrocarbons as a distinctive biomass source.

scholarly journals CRYSTALLINE AND HETEROCYCLYC AROMATIC COMPOUNDS IN GEORGIAN PETROLEUM

World Science ◽  
2021 ◽  
Author(s):  
Natela Khetsuriani ◽  
Vladimer Tsitsishvili ◽  
Elza Topuria ◽  
Irina Mchedlishvili ◽  
Zaza Molodinashvili
Keyword(s):  
Polycyclic Aromatic Hydrocarbons ◽  
Aromatic Hydrocarbons ◽  
Aromatic Compounds ◽  
High Intensity ◽  
Oil Wells ◽  
Automated System ◽  
Polycyclic Aromatic ◽  
The Individual ◽  
First Time ◽  
Individual Composition

The individual composition of polycyclic aromatic hydrocarbons has been studied in high boiling (340–590°) fractions of Norio oil (wells 200, 201) which are characterized by high content of aromatic hydrocarbons and high intensity fluorescence. The eluates obtained by adsorption fractionation of aromatic hydrocarbons separated by aniline and concentrates of their crystalline components have been studied by GC, MS and GC-MS methods. For analysis of the obtained data an automated system of mass deconvolution and identification (AMDIS) was used. In the eluates under investigation the following structures were identified: indenes, tetralines, dinaphtilbenzenes, naphthalenes, fluorenes, phenantrenes, antracenes, mono- and polyalkyl derivatives ofnaphtofluorene and phenantrene, and terpeniles. In crystal samples of the eluates the banzantracene, chrizene, their methyl-, dimethyl and trymethylanaloges, phenantrene derivatives, antracenes and pyrenes were identified. The heterocyclic analogues of polycyclic aromatic hydrocarbons likemethylbenzoanthracenes, benzonaphthothiophenes, benzocarbazoles and dibenzthiophene were identified in Georgian oils for the first time.

scholarly journals Sugar Alcohols and Organic Acids Synthesis in Yarrowia lipolytica: Where Are We?

2020 ◽  
Vol 8 (4) ◽  
pp. 574 ◽  
Author(s):  
Patrick Fickers ◽  
Hairong Cheng ◽  
Carol Sze Ki Lin
Keyword(s):  
Organic Acids ◽  
Yarrowia Lipolytica ◽  
Current Knowledge ◽  
Raw Materials ◽  
Low Cost ◽  
Industrial Wastes ◽  
Sugar Alcohols ◽  
Cell Factories ◽  
Main Challenge ◽  
Pharmaceutical Industries

Sugar alcohols and organic acids that derive from the metabolism of certain microorganisms have a panoply of applications in agro-food, chemical and pharmaceutical industries. The main challenge in their production is to reach a productivity threshold that allow the process to be profitable. This relies on the construction of efficient cell factories by metabolic engineering and on the development of low-cost production processes by using industrial wastes or cheap and widely available raw materials as feedstock. The non-conventional yeast Yarrowia lipolytica has emerged recently as a potential producer of such metabolites owing its low nutritive requirements, its ability to grow at high cell densities in a bioreactor and ease of genome edition. This review will focus on current knowledge on the synthesis of the most important sugar alcohols and organic acids in Y. lipolytica.

scholarly journals Preliminary Research Concerning the Determination of Beer Wort Flavor Compounds During Primary Fermentation

2010 ◽  
Vol 67 (2) ◽  
Author(s):  
Delia MICHIU ◽  
Maria TOFANA ◽  
Elena MUDURA ◽  
Florina MUNTEAN
Keyword(s):  
Organic Acids ◽  
Aromatic Compounds ◽  
Raw Materials ◽  
Flavor Compounds ◽  
Higher Alcohols ◽  
Secondary Products ◽  
Vast Array ◽  
Preliminary Research ◽  
The Moment

Generally beer contain numerous aromatic compounds, some naturally present in the raw materials and some formed during processing. In this work were analysed beer wort flavor compounds, during primary fermentation, taking into study two beer wort types: one obtained from Pilsner malt (100%), other from a mixture of Pilsner malt (80%) and Caramel Malt (20%). An Shimadzu GC/MS-QP2010 equipment was used for samples analysis. The major compounds founded in beer wort samples, was derived from a vast array of compounds that arise from a number of sources. In this way, during fermentation and conditioning we founded different categories of secondary products: higher alcohols, esters, vicinal diketones (VDK), organic acids and aldehydes. The concentration of this aroma compounds depends on malt type and the moment of wort examination. Was noticed that some compounds disappeared and others appeared, from the day to day of primary fermentation.

ON THE PROCESSING OF HEMATITE RAW MATERIALS AT THE KRYVOROZHSK MINING AND PROCESSING PLANT OF OXIDIZED ORE ON TECHNOLOGY OF DIRECT RECOVERY ITmk3

2018 ◽  
pp. 32-37
Author(s):  
P.I. Loboda ◽  
Younes Razaz ◽  
S. Grishchenko
Keyword(s):  
Magnetic Properties ◽  
Cast Iron ◽  
Iron Content ◽  
Raw Materials ◽  
Direct Reduction ◽  
Processing Plant ◽  
High Iron Content ◽  
High Iron ◽  
Direct Restoration ◽  
First Time

Purpose. To substantiate the efficiency of processing hematite raw materials at the Krivoy Rog Mining and Processing Plant of Oxidized Ores using the direct reduction technology itmk3®. Metodology. Analysis of the results of the itmk3® direct restoration technology developed by Kobe Steel Ltd., Japan and Hares Engineering GmbX, Austria, with a view to using it to process Krivbass hematite ores into granulated iron (so-called “nuggets”). Findings. The involvement in the production of hematite ores (oxidized quartzite) of Krivbass with high iron content, but with low magnetic properties for their processing into granular cast iron is grounded. Originality. The use of itmk3® direct reduction technology from Kobe Steel Ltd., Japan and Hares Engineering GmbH, Austria for the processing of Krivbass hematite ores into granular cast iron is justified for the first time. Practical value. The efficiency of the use of hematite ores (oxidized quartzite) has been substantiated, which can significantly reduce the costs in the mining cycle for the economical production of metallurgical products.

PERSPECTIVE DEVELOPMENT OF WORLD PRODUCTION OF FEEDS FOR AQUACULTURE: ALTERNATIVE SOURCES OF RAW MATERIALS

2017 ◽  
pp. 67-78
Author(s):  
Lina Yurievna Lagutkina
Keyword(s):  
Raw Materials ◽  
Limiting Factors ◽  
Current Status ◽  
Feed Production ◽  
The World ◽  
Market Trends ◽  
Alternative Sources ◽  
World Industry ◽  
First Time ◽  
A Current

The author of the article discloses the prospects of development of the world feed production for aquaculture based on the analysis of key innovative technological and market trends. The author specifies that shortage, high cost, low ecological compatibility of traditional raw materials - fish flour - are among major limiting factors in the development of production of feeds for aquaculture. This fact, in turn, limits sustainable development of aquaculture both in Russia, and in the world in general. The article presents the overview of a current status of the world industry of feed production in aquaculture, where the regional situation is studied, as well. For the first time, there is given the outlook of innovative technologies in feed production based on the alternative sources of protein (on the example of projects of leading aquabiotechnological companies) which will determine industry’s objectives for the mid-term perspective.

Safety assessment of biological raw materials in the system of medicines quality assurance

2020 ◽  
pp. 63-72
Author(s):  
Yu. Olefir ◽  
E. Sakanyan ◽  
I. Osipova ◽  
V. Dobrynin ◽  
M. Smirnova ◽  
...  
Keyword(s):  
Raw Materials ◽  
State Level ◽  
The State ◽  
Spongiform Encephalopathy ◽  
Safety Standards ◽  
Wide Range ◽  
The Russian Federation ◽  
Key Aspects ◽  
First Time ◽  
Medicines Quality

The entry of a wide range of biotechnological products into the pharmaceutical market calls for rein-forcement of the quality, efficacy and safety standards at the state level. The following general monographs have been elaborated for the first time to be included into the State Pharmacopoeia of the Russian Federation, XIV edition: "Viral safety" and "Reduction of the risk of transmitting animal spongiform encephalopathy via medicinal products". These general monographs were elaborated taking into account the requirements of foreign pharmacopoeias and the WHO recommendations. The present paper summarises the key aspects of the monographs.

scholarly journals Mineralogy and Geochemistry of Ultramafic Rocks from Rachoni Magnesite Mine, Gerakini (Chalkidiki, Northern Greece)

Minerals ◽  
2020 ◽  
Vol 10 (11) ◽  
pp. 934
Author(s):  
Evangelos Tzamos ◽  
Micol Bussolesi ◽  
Giovanni Grieco ◽  
Pietro Marescotti ◽  
Laura Crispini ◽  
...  
Keyword(s):  
Raw Materials ◽  
Mining Area ◽  
Ultramafic Rocks ◽  
Northern Greece ◽  
Icp Ms ◽  
Time In Literature ◽  
History Of ◽  
First Time ◽  
The Eu

The importance of magnesite for the EU economy and industry is very high, making the understanding of their genesis for the exploration for new deposits a priority for the raw materials scientific community. In this direction, the study of the magnesite-hosting ultramafic rocks can be proved very useful. For the present study, ultramafic rock samples were collected from the magnesite ore-hosting ophiolite of the Gerakini mining area (Chalkidiki, Greece) to investigate the consecutive alteration events of the rocks which led to the metallogenesis of the significant magnesite ores of the area. All samples were subjected to a series of analytical methods for the determination of their mineralogical and geochemical characteristics: optical microscopy, XRD, SEM, EMPA, ICP–MS/OES and CIPW normalization. The results of these analyses revealed that the ultramafic rocks of the area have not only all been subjected to serpentinization, but these rocks have also undergone carbonation, silification and clay alteration. The latter events are attributed to the circulation of CO2-rich fluids responsible for the formation of the magnesite ores and locally, the further alteration of the serpentinites into listvenites. The current mineralogy of these rocks was found to be linked to one or more alteration event that took place, thus a significant contribution to the metallo- and petrogenetic history of the Gerakini ophiolite has been made. Furthermore, for the first time in literature, Fe inclusions in olivines from Greece were reported.

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