scholarly journals Panbio antigen rapid test is reliable to diagnose SARS-CoV-2 infection in the first 7 days after the onset of symptoms

2020 ◽  
Author(s):  
Manuel Linares ◽  
Ramón Pérez Tanoira ◽  
Juan Romanyk ◽  
Felipe Pérez García ◽  
Peña Gómez-Herruz ◽  
...  
Keyword(s):  
Acute Infection ◽  
Rapid Test ◽  
High Sensitivity ◽  
Rapid Identification ◽  
Laboratory Method ◽  
Molecular Techniques ◽  
Special Equipment ◽  
Viral Loads ◽  
High Throughput Method ◽  
Polymerase Chain

Background: Real-time reverse transcription polymerase chain reaction (RT-qPCR) is the current recommended laboratory method to diagnose SARS-CoV-2 acute infection, several factors such as requirement of special equipment, time consuming, high cost and skilled staff limit the use of these time-consuming molecular techniques. A more rapid and high-throughput method is in growing demand Methods: Evaluate the performances of the Panbio COVID-19 AG Rapid Test Device (Nasopharyngeal), a rapid immunochromatographic test for the detection of SARS-CoV-2 antigen, in comparison to RT-qPCR. Results: The RDT evaluated in this study showed a high sensitivity and specificity in samples mainly obtained during the first week of symptoms and with high viral loads. Conclusions: This assay seems to be an effective strategy for controlling the COVID-19 pandemic for the rapid identification and isolation of SARS-CoV-2 infected patients.

scholarly journals The Correlation of Rapid Antibody Results with SARS-CoV-2 PCR in COVID-19 Patients in Ulin General Hospital Banjarmasin

2021 ◽  
Vol 7 (3) ◽  
pp. 100
Author(s):  
Isa Ansori ◽  
Soraya Riefani ◽  
Ira Nurrasyidah
Keyword(s):  
General Hospital ◽  
Viral Infections ◽  
Rapid Test ◽  
High Sensitivity ◽  
Test Results ◽  
Chi Square ◽  
The Social ◽  
Chi Square Test ◽  
Polymerase Chain ◽  
Rapid Antibody

Introduction: Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) is the cause of clinical disease, better known as COVID-19. The most common method to detect COVID-19 is serological testing of IgM and IgG in response to viral infections using rapid diagnostic test (RDT). Several other guidelines consider polymerase chain reaction (PCR) as the gold standard for diagnosis becausePCR has high sensitivity and specificity values in detecting SARS-CoV-2.Methods: This was a descriptive analytical study. The samples were taken from medical records of COVID-19 patients in Ulin General Hospital Banjarmasin from March to October 2020. Statistical Package for the Social Sciences (SPSS) 16.0 software and Chi-Square test were used for data analysis.Results: From 751 COVID-19 patients, 408 patients (54.32%) had rapid antibody with positive PCR, 132 patients (17.57%) had reactive rapid antibody with negative PCR, 152 patients (20.23%) had non-reactive rapid antibody with positive PCR, and 59 patients (7.85%) had non-reactive rapid antibody with negative PCR. The rapid antibody had sensitivity of 72.85% and specificity of 30.89%. From Chi-Square test, reactive rapid antibody was not correlated with PCR positive results; values of p = 0.320, odds ratio (OR) 1.20.Conclusion: The rapid test antibody could not be recommended as a diagnostic tool. In this study, it was also found that there was no relationship between reactive rapid test results and positive SARS-CoV PCR.

scholarly journals Colorimetric LAMP Molecular Method for Immediate Detection of Three Periodontal Pathogens

2020 ◽  
Author(s):  
Sumaya A.S.M. Al-Hamdoni ◽  
Amera M.M. Al-Rawi
Keyword(s):  
Bacterial Species ◽  
Rapid Identification ◽  
Molecular Techniques ◽  
Diagnostic Tools ◽  
Lamp Method ◽  
Molecular Technique ◽  
Phenol Red ◽  
Periodontal Pathogens ◽  
Special Equipment ◽  
Phenotypic Characters

Abstract Background and Purpose: For enhancing the efficiency of diagnostic tools and overcome drawbacks faced in diagnosis attempt, molecular methods have been developed aid accurate and rapid identification of bacterial species particularly those which are difficult to study with. The current study searched to find a more specific and rapid method serves researchers for the identification of periodontal pathogens and overcome disturbances encountered in study such anaerobic fastidious organisms. Methods and Results: Three periodontal pathogens, Porphyromonas gingivalis , Tannerella forsythia and Treponema denticola were characterized and identified by phenotypic features and Loop- Mediated Isothermal Amplification (LAMP) techniques which involved 4 sets of primers targeted to 16SrRNA genes and loop primers and the Colorimetric Master Mix containing Bst DNA polymerase and Phenol Red for the detection of amplicon formation. There was a variance in phenotypic characters of the isolates of the same species. LAMP, as a novel molecular technique was of usefulness value in identifying the target weather as extracted DNA or whole cells in a high specific and very rapid manner within 30 min. by visual reading of the results. Conclusion: It is strongly recommended the use of the novel LAMP method in researches work with periodontal pathogens as its advantages make it superior to other molecular techniques in respects of a higher specificity, rapidity, sensitivity and overcome DNA extraction step and special equipment in that it can be used at chair- side identification.

scholarly journals Potential Use of Antigen-Based Rapid Test for SARS-CoV-2 in Respiratory Specimens in Low-Resource Settings in Egypt for Symptomatic Patients and High-Risk Contacts

2020 ◽  
Author(s):  
Abeer Mohamed Abdelrazik ◽  
Shahira Morsy Elshafie ◽  
Hossam M Abdelaziz
Keyword(s):  
Test Performance ◽  
Healthcare Professionals ◽  
Rapid Test ◽  
University Hospital ◽  
Antigen Test ◽  
Rt Pcr ◽  
Viral Loads ◽  
Viral Transport Medium ◽  
Public Health Challenges ◽  
Polymerase Chain

Abstract Objective Because of the rapidly emerging SARS-CoV-2 pandemic and its wide public health challenges, rapid diagnosis is essential to decrease the spread. Antigen-based rapid detection tests are available; however, insufficient data about their performance are available. Methods The lateral-flow immunochromatographic BIOCREDIT COVID-19 antigen test was evaluated using nasopharyngeal swabs in a viral transport medium from patients with confirmed infection, contacts, and exposed healthcare professionals at Fayoum University Hospital in Egypt. Test performance was determined in comparison to the SARS-CoV-2 real-time reverse-transcription polymerase chain reaction (RT-PCR) test. Results Three hundred ten specimens from 3 categories—patients with confirmed diagnoses of COVID-19, contacts, and exposed healthcare professionals—were included; 188 specimens were RT-PCR-positive, from which 81 were detected by rapid antigen test. Overall sensitivity was 43.1%. Sensitivity was significantly higher in specimens with high viral loads. Conclusion Poor sensitivity of the BIOCREDIT COVID-19 test does not permit its use for diagnosis, and it can only be used in conjunction with RT-PCR for screening.

scholarly journals Time scale performance of rapid antigen testing for SARS-COV-2: evaluation of ten rapid antigen assays

2021 ◽  
Author(s):  
Zakarya Abusrewil ◽  
Inas M Alhudiri ◽  
Hamza Kaal ◽  
Salah Edin El Meshri ◽  
Fawzi O Ebrahim ◽  
...  
Keyword(s):  
Sensitivity And Specificity ◽  
Symptom Onset ◽  
False Negative ◽  
Rapid Test ◽  
High Sensitivity ◽  
Molecular Diagnostic ◽  
Nasopharyngeal Swab ◽  
Viral Loads ◽  
Biotechnology Research ◽  
Antigen Tests

Background: There is a great demand for more rapid tests for SARS-COV-2 detection to reduce waiting time, boost public health strategies for combating disease, decrease costs, and prevent overwhelming laboratory capacities. This study was conducted to assess the performance of 10 antigen-based rapid assays for the detection of SARS-CoV-2 in nasopharyngeal swab specimens. Methods: We analyzed 231 nasopharyngeal samples collected from October 2020-December 2020, from suspected COVID-19 cases and contacts of positive cases at Biotechnology Research Center laboratories, Tripoli, Libya. The performance of 10 COVID-19 Ag rapid test devices (Fluorecare, ESPLINE, RapiGen, Abbott Panbio, Flowflex, Acon, Assut Europe, Orient Gene, CerTest, Bioperfectus, AMP) for the detection of SARS-CoV-2 antigen was compared to RT-qPCR. Results: Among the 108 positive samples detected by RT-qPCR, the COVID-19 antigen (Ag) tests detected 83, giving a sensitivity of 76.85% (95% CI, 67.75- 84.43). 161 patients were symptomatic. The median cycle threshold was 25. The mean duration from symptom onset was 6.6 plus/minus 4.3 days. Sensitivity and specificity during the first 6 days of symptoms and in samples with high viral loads ct<25, was 96.4%. No false positives were detected by any of the Ag tests utilized in this study. False negative samples had a median Ct of 34 and average duration of onset of symptoms of 11.3 days (range=5-20). Conclusions: Rapid antigen test diagnosis has high sensitivity and specificity in early disease when patients present less than 7 days of symptom onset. Patients are encouraged to test as soon as they get COVID-19 related symptoms within 1 week and to seek medical advice within 24 hrs. if they develop disturbed smell/taste. The use of rapid antigen tests is important for controlling COVID-19 pandemic and reducing burden on molecular diagnostic laboratories.

scholarly journals Tuberculosis in Birds: Insights into theMycobacterium aviumInfections

2011 ◽  
Vol 2011 ◽  
pp. 1-14 ◽  
Author(s):  
Kuldeep Dhama ◽  
Mahesh Mahendran ◽  
Ruchi Tiwari ◽  
Shambhu Dayal Singh ◽  
Deepak Kumar ◽  
...  
Keyword(s):  
Animal Health ◽  
Clinical Manifestations ◽  
Tuberculin Test ◽  
Rapid Identification ◽  
Molecular Techniques ◽  
Human Beings ◽  
Pet Birds ◽  
Polymerase Chain ◽  
World Organization

Tuberculosis, a List B disease of World Organization for Animal Health, caused byM. aviumorM. genavensepredominantly affects poultry and pet or captive birds. Clinical manifestations in birds include emaciation, depression and diarrhea along with marked atrophy of breast muscle. Unlike tuberculosis in animals and man, lesions in lungs are rare. Tubercular nodules can be seen in liver, spleen, intestine and bone marrow. Granulomatous lesion without calcification is a prominent feature. The disease is a rarity in organized poultry sector due to improved farm practices, but occurs in zoo aviaries. Molecular techniques like polymerase chain reaction combined with restriction fragment length polymorphism and gene probes aid in rapid identification and characterization of mycobacteria subspecies, and overcome disadvantages of conventional methods which are slow, labour intensive and may at times fail to produce precise results.M. avium subsp. aviumwith genotypeIS901+ andIS1245+ causes infections in animals and human beings too. The bacterium causes sensitivity in cattle to the tuberculin test. The paper discusses in brief theM. aviuminfection in birds, its importance in a zoonotic perspective, and outlines conventional and novel strategies for its diagnosis, prevention and eradication in domestic/pet birds and humans alike.

scholarly journals Copro-PCR in the detection and confirmation of Toxoplasma gondii oocysts in feces of stray and domiciled cats

2021 ◽  
Vol 30 (2) ◽  
Author(s):  
Jaqueline Ataíde Silva Lima da Igreja ◽  
Hanstter Hallison Alves Rezende ◽  
Jade de Oliveira Melo ◽  
João Luís Garcia ◽  
Felippe Danyel Cardoso Martins ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Toxoplasma Gondii ◽  
Genetic Material ◽  
High Sensitivity ◽  
Molecular Techniques ◽  
Molecular Methods ◽  
Chain Reaction ◽  
Highly Sensitive ◽  
Polymerase Chain ◽  
Fecal Tests

Abstract Molecular methods such as Copro-PCR stand out in the diagnosis of T. gondii, because they are highly sensitive and specific, and can distinguish T. gondii from other morphologically similar coccids. The purpose was the detection of Toxoplasma gondii copro-prevalence by polymerase chain reaction in 149 fecal samples from stray and domiciled cats, using three distinct markers (B5-B6, 18S and 529bp RE). Oocysts of T. gondii/H. hammondi were detected in 15.4% by parasitology fecal tests (PFT), and 4% of these oocysts were positively identified as T. gondii by Copro-PCR. The presence of T. gondii genetic material was detected in 16.1%, but 12% of the samples that tested positive by Copro-PCR were negative in PFT. Samples with discordant results were subjected to a new Copro-PCR with 18S marker and a 529, and of the 17 samples, 9 contained T. gondii genetic material. A comparison of the PFT and the molecular methods showed the latter was more sensitive, since it detected 22.1% while the PFT detected 15.4%. Demonstrating the high sensitivity and specificity of the Copro-PCR, particularly with the association of primers (k=0.809), but also confirms the importance of using molecular techniques in laboratories, since Copro-PCR was able to detect samples considered negative by PFT.

scholarly journals Pemeriksaan Diagnostik COVID-19 : Studi Literatur

2020 ◽  
Vol 5 (1) ◽  
Author(s):  
Halbina Famung Halmar ◽  
Nur Febrianti ◽  
Maria Kurnyata Rante Kada
Keyword(s):  
Initial Phase ◽  
Rapid Test ◽  
High Sensitivity ◽  
Inclusion Criteria ◽  
Rt Pcr ◽  
Literature Study ◽  
Chain Reaction ◽  
Chest Examination ◽  
Combination Test ◽  
Polymerase Chain

Background: The purpose of this literature study is to find out which diagnostic tests are used to detect COVID-19. Method: Articles collected from 2020 using 3 databases (Pubmed, Science Direct and Google Scholar) obtained 14 articles that fit the inclusion criteria. Results: CT-Chest examination has a high sensitivity for diagnosis of COVID-19 compared to Real-Time Polymerase Chain Reaction (RT-PCR) in the initial phase. The IgG and IgM combination test has better utility and sensitivity compared to the IgG or IgM test alone. Conclusion: Examinations carried out to detect COVID-19 accurately and precisely must combine RT-PCR, CT-Chest and Rapid Test.

Rapid identification of Escherichia coli microcin J25 producing strains using polymerase chain reaction and colony blot hybridization

2001 ◽  
Vol 47 (9) ◽  
pp. 877-882 ◽  
Author(s):  
Mariela Duarte ◽  
Gilles Cottenceau ◽  
Véronique Portrait ◽  
Anne-Marie Pons
Keyword(s):  
Escherichia Coli ◽  
Polymerase Chain Reaction ◽  
Blot Hybridization ◽  
Rapid Identification ◽  
Molecular Techniques ◽  
Chain Reaction ◽  
Gene Encoding ◽  
Bacterial Populations ◽  
Polymerase Chain ◽  
Microcin J25

To screen, isolate, and characterize bacterial populations producing microcin J25, we report here two rapid, reliable, and sensitive methods, using polymerase chain reaction and colony blot hybridization with a digoxigenin-labelled probe. A sample of 26 Escherichia coli strains isolated from poultry intestinal contents was evaluated to detect the sequence of mcjA, the gene encoding the MccJ25 precursor. The two molecular techniques were compared with the commonly used cross-immunity tests. They generate accurate data with no obvious cross-reactions with other microcins. The results display that the producers of MccJ25 were widely distributed in the poultry intestinal habitat. The applications of these molecular methods will be useful in future studies of microcinogenic populations, and thus contribute to understand the relationships within the complex intestinal microbial ecosystem.Key words: microcin J25, microcinogenic strains detection, digoxigenin-labelled probe, colony hybridization, polymerase chain reaction.

Identification of Microorganisms by Modern Analytical Techniques

2017 ◽  
Vol 100 (6) ◽  
pp. 1607-1623 ◽  
Author(s):  
Bogusław Buszewski ◽  
Agnieszka Rogowska ◽  
Paweł Pomastowski ◽  
Michał Złoch ◽  
Viorica Railean-Plugaru
Keyword(s):  
Analytical Techniques ◽  
Rapid Identification ◽  
Molecular Techniques ◽  
Ionization Time ◽  
Detection And Identification ◽  
Wide Range ◽  
Microorganism Identification ◽  
Capillary Zone ◽  
Identification Of Bacteria ◽  
Polymerase Chain

Abstract Rapid detection and identification of microorganisms is a challenging and important aspect in a wide range of fields, from medical to industrial, affecting human lives. Unfortunately, classical methods of microorganism identification are based on time-consuming and labor-intensive approaches. Screening techniques require the rapid and cheap grouping of bacterial isolates; however, modern bioanalytics demand comprehensive bacterial studies at a molecular level. Modern approaches for the rapid identification of bacteria use molecular techniques, such as 16S ribosomal RNA gene sequencing based on polymerase chain reaction or electromigration, especially capillary zone electrophoresis and capillary isoelectric focusing. However, there are still several challenges with the analysis of microbial complexes using electromigration technology, such as uncontrolled aggregation and/or adhesion to the capillary surface. Thus, an approach using capillary electrophoresis of microbial aggregates with UV and matrix-assisted laser desorption ionization time-of-flight MS detection is presented.

scholarly journals A comparative evaluation of two chromogenic media for surveillance of carbapenem-resistant Enterobacterales with non-carbapenemase-producing or carbapenemase-producing strains other than blaKPC: blaGES-5, blaNDM-1 or blaVIM-2

2019 ◽  
Vol 43 (3) ◽  
pp. 173-176
Author(s):  
Chang-Hun Park
Keyword(s):  
Rapid Detection ◽  
Comparative Evaluation ◽  
Susceptibility Testing ◽  
Rapid Identification ◽  
Surveillance Program ◽  
Contact Precaution ◽  
Chain Reaction ◽  
Carbapenem Resistant ◽  
Control And Prevention ◽  
Polymerase Chain

Abstract Background Infections caused by carbapenem-resistant Enterobacterales (CREs) are an emerging problem associated with high rates of morbidity and mortality. CREs are divided into two categories (carbapenemase-producing [CP] CRE and non-CP CRE). The most prevalent carbapenemase produced by Enterobacterales is Klebsiella pneumoniae carbapenemase (KPC) in Korea. Rapid identification of CREs is clinically important in infection control precaution. We compared the performance of two chromogenic media (chromID CARBA agar and CHROMagar KPC agar) for non-CP CREs or CP CREs with blaGES-5, blaNDM-1 or blaVIM-2 in a Korean hospital. Methods The study was carried out during a 3-month period from April to June 2017 during the surveillance program for CRE colonization. Antimicrobial susceptibility testing (AST) and polymerase chain reaction (PCR) were performed at the Korean Centers for Disease Control and Prevention. Results A total of 45 rectal swabs from 42 hospitalized patients were examined. Sensitivity of both chromID CARBA and CHROMagar KPC were 100% for CP CREs; and 50% and 100% for non-CP CREs, respectively. Specificity of chromID CARBA and CHROMagar KPC were 89.2% and 70.3% for CP CRE, respectively; and 76.9% and 66.7% for non-CP CRE, respectively. Conclusions The CHROMagar KPC is useful to monitor non-CP and CP CREs. The chromID CARBA is efficient for rapid detection of CP CREs requiring high contact precaution.

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