scholarly journals Method development and characterization of the low molecular weight peptidome of human wound fluids

2020 ◽  
Author(s):  
Mariena J.A. van der Plas ◽  
Jun Cai ◽  
Jitka Petrlova ◽  
Karim Saleh ◽  
Sven Kjellström ◽  
...  
Keyword(s):  
Wound Healing ◽  
Method Development ◽  
Healing Process ◽  
Unmet Need ◽  
Wound Healing Process ◽  
Promising Tool ◽  
Physiological Processes ◽  
Post Surgery ◽  
Surgical Wounds ◽  
Novel Biomarkers

AbstractWound infections are significant challenges globally, and there is an unmet need for better diagnosis of wound healing status and infection. The wound healing process is characterized by proteolytic events that are the result of basic physiological processes involving coagulation and complement activation, but also dysfunctional activations by endogenous and bacterial proteases. Peptides, downstream reporters of these proteolytic actions, could therefore serve as a promising tool for diagnosis of wound healing and infection. In the present study, we demonstrate a method for the characterisation of the complete peptidome of human wound fluids. We compare acute non-infected wound fluids obtained post-surgery with plasma samples and find significantly higher protein and peptide numbers in wound fluids, which typically were also smaller in size as compared to plasma-derived peptides. Finally, we analyse wound fluids collected from dressings after facial skin graft surgery and compare three uninfected and normally healing surgical wounds with three inflamed and S. aureus infected wounds. The results identify unique peptide patterns of various selected proteins including coagulation and complement factors, as well as proteases and antiproteinases. Together, the work defines a workflow for analysis of peptides derived from human wound fluids and demonstrate a proof-of-concept that such wound fluids can be used for analysis of qualitative differences of peptide patterns derived from wound fluids on larger patient cohorts, providing novel biomarkers for wound healing and infection.

scholarly journals Method development and characterisation of the low-molecular-weight peptidome of human wound fluids

eLife ◽  
2021 ◽  
Vol 10 ◽  
Author(s):  
Mariena JA van der Plas ◽  
Jun Cai ◽  
Jitka Petrlova ◽  
Karim Saleh ◽  
Sven Kjellström ◽  
...  
Keyword(s):  
Wound Healing ◽  
Method Development ◽  
Healing Process ◽  
Wound Healing Process ◽  
Proof Of Concept ◽  
Normal Wound Healing ◽  
Promising Tool ◽  
Healing Wounds ◽  
High Degree ◽  
First Time

The normal wound healing process is characterised by proteolytic events, whereas infection results in dysfunctional activations by endogenous and bacterial proteases. Peptides, downstream reporters of these proteolytic actions, could therefore serve as a promising tool for diagnosis of wounds. Using mass-spectrometry analyses, we here for the first time characterise the peptidome of human wound fluids. Sterile post-surgical wound fluids were found to contain a high degree of peptides in comparison to human plasma. Analyses of the peptidome from uninfected healing wounds and Staphylococcus aureus -infected wounds identify unique peptide patterns of various proteins, including coagulation and complement factors, proteases, and antiproteinases. Together, the work defines a workflow for analysis of peptides derived from wound fluids and demonstrates a proof-of-concept that such fluids can be used for analysis of qualitative differences of peptide patterns from larger patient cohorts, providing potential biomarkers for wound healing and infection.

Identifying New Pathways and Targets for Wound Healing and Therapeutics from Natural Sources

2021 ◽  
Vol 18 ◽  
Author(s):  
Xinchi Feng ◽  
Jinsong Hao
Keyword(s):  
Wound Healing ◽  
Molecular Mechanisms ◽  
Chronic Wounds ◽  
Wound Care ◽  
Healing Process ◽  
Unmet Need ◽  
Wound Dressings ◽  
Wound Healing Process ◽  
Natural Sources ◽  
Viable Approach

: Chronic wounds remain a significant public problem and the development of wound treatments has been a research focus for the past few decades. Despite advances in the products derived from endogenous substances involved in a wound healing process (e.g. growth factors, stem cells, and extracellular matrix), effective and safe wound therapeutics are still limited. There is an unmet need to develop new therapeutics. Various new pathways and targets have been identified and could become a molecular target in designing novel wound agents. Importantly, many existing drugs that target these newly identified pathways could be repositioned for wound therapy, which will facilitate fast translation of research findings to clinical applications. This review discusses the newly identified pathways/targets and their potential uses in the development of wound therapeutics. Some herbs and amphibian skins have been traditionally used for wound repairs and their active ingredients have been found to act in these new pathways. Hence, screening these natural products for novel wound therapeutics remains a viable approach. The outcomes of wound care using natural wound therapeutics could be improved if we can better understand their cellular and molecular mechanisms and fabricate them in appropriate formulations, such as using novel wound dressings and nano-engineered materials. Therefore, we also provide an update on the advances in the wound therapeutics from natural sources. Overall, this review offers new insights into novel wound therapeutics.

scholarly journals Pengaruh Program Ambulasi Dini Terhadap Proses Penyembuhan Luka Pasien Post Operasi Di RSUD dr Adjidarmo Rangkasbitung

2020 ◽  
Vol 7 (2) ◽  
pp. 85
Author(s):  
Rodi Widiantoro
Keyword(s):  
Wound Healing ◽  
Healing Process ◽  
Intervention Group ◽  
Sampling Technique ◽  
Major Surgery ◽  
Control Group ◽  
Wound Healing Process ◽  
Early Ambulation ◽  
Postoperative Wound ◽  
Surgical Wounds

Luka dan nyeri akibat dari pembedahan membuat pasien tidak mau bergerak sehingga banyak luka operasi yang mengalami gangguan proses penyembuhan luka, untuk membantu dalam proses penyembuhan luka operasi dapat dilakukan teknik ambulasi.  Ambulasi dini  adalah salah satu faktor yang dapat mempengaruhi penyembuhan luka pasca Operasi serta dapat mengurangi risiko komplikasi.  Tujuan  untuk  melihat  pengaruh program ambulasi dini  terhadap perubahan   proses  penyembuhan  luka  pada  pasien  paska  operasi. Rancangan penelitian quasi-eksperimental  dengan  jumlah sampel 99  responden, 75 responden (kelompok intervensi) dan 24 responden (kelompok control),  yang  diambil  secara  purposive  sampling  technique.  Data  dikumpulkan  melalui  kuesioner  dan  lembar  observasi, kemudian dianalisis dengan uji  regresi logistik ordinal. Hasil menunjukkan rata-rata usia 31 tahun (kontrol), 45 tahun (intervensi), jumlah pasien operasi pada laki-laki (58%), operasi mayor (78%), HB normal: (73,7%). Penyembuhan luka yang adekuat kelompok intervensi  sebanyak 50 responden (66,7%)   sedangkan pada kelompok kontrol sembuh dengan adekuat 6 responden (25%).  Uji beda independen pasien ambulasi dini dengan proses penyembuhan luka post operasi nilai (p=0,000). Disimpulkan bahwa  program ambulasi dini berpengaruh terhadap penyembuhan luka post operasi. Penelitian ini merekomendasikan penelitian lebih lanjut dan penggunaan latihan ini sebagai salah satu intervensi mandiri perawat dalam memberikan asuhan keperawatan pada pasien paska operasi. Kata kunci:  ambulasi dini;  proses penyembuhan luka; operasiINFLUENCE OF EARLY AMBULATION PROGRAM IN WOUND HEALING OF POST OPERATIVE PATIENTS IN RSUD dr. ADJIDARMO RANGKASBITUNG  ABSTRACT The wounds and pain resulting from surgery make the patient unable to move so that many surgical wounds experience interference with the wound healing process, to assist in the healing process of surgical wounds an ambulation technique can be used. Early ambulation is one of the factors that can affect postoperative wound healing and can reduce the risk of complications. The aim of the sudy was to determine the effect of early ambulation programs in the wound healing process of postoperative patients. A quasy-experimental research design was used with a sample size of 99 respondents, 75 respondents (intervention group) and 24 respondents (control group), which were taken by purposive technique sampling. The data were collected through questionnaires and observation sheets, and then analyzed using ordinal logistic regression test. The results showed that the mean age was 31 years (control), 45 years (intervention), the number of male patients with surgery is 58%, major surgery is 78%, and normal HB is (73.7%). The adequate wound healing in the intervention group is 50 respondents (66.7%) while the control group healed adequately for 6 respondents (25%). Independent difference test of early ambulation patients with postoperative wound healing process value (p = 0.000). It was concluded that the early ambulation program had an effect on postoperative wound healing. This study recommends further research and the use of this exercise as one of the nurses' independent interventions in providing nursing care to postoperative patients.Keywords: early ambulation; wound healing process; of surgery

scholarly journals Topical Gynura procumbens as a Novel Therapeutic Improves Wound Healing in Diabetic Mice

Plants ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 1122
Author(s):  
Nutda Sutthammikorn ◽  
Volaluck Supajatura ◽  
Hainan Yue ◽  
Miho Takahashi ◽  
Sunee Chansakaow ◽  
...  
Keyword(s):  
Wound Healing ◽  
Growth Factor ◽  
Transforming Growth Factor ◽  
Therapeutic Option ◽  
Healing Process ◽  
Unmet Need ◽  
New Drugs ◽  
Wound Healing Process ◽  
Diabetic Mice ◽  
Diabetic Wounds

Nonhealing wounds are major socioeconomic challenges to healthcare systems worldwide. Therefore, there is a substantially unmet need to develop new drugs for wound healing. Gynura procumbens, a herb found in Southeast Asia, may be an effective therapeutic for nonhealing diabetic wounds. The aim of this study was to evaluate the efficacy of G. procumbens on wound healing in the diabetic milieu. G. procumbens extract was obtained using 95% ethanol and its components were determined by thin layer chromatography. Diabetes was induced in mice using streptozotocin. We found that G. procumbens extract contained stigmasterol, kaempferol and quercetin compounds. Topical application of G. procumbens on the wounded skin of diabetic mice accelerated wound healing and induced the expression of angiogenin, epidermal growth factor, fibroblast growth factor, transforming growth factor and vascular endothelial growth factor. Furthermore, G. procumbens promoted in vitro wound healing and enhanced the migration and/or proliferation of human endothelial cells, fibroblasts, keratinocytes and mast cells cultured in diabetic conditions. Finally, G. procumbens promoted vascular formation in the diabetic mice. To the best of our knowledge, this is the first study that evaluates in vivo wound healing activities of G. procumbens and activation of cells involved in wound healing process in diabetic conditions. The findings that G. procumbens accelerates wound healing and activates cells involved in the wound healing process suggest that G. procumbens might be an effective alternative therapeutic option for nonhealing diabetic wounds.

scholarly journals Nanomaterials for Wound Dressings: An Up-to-Date Overview

Molecules ◽  
2020 ◽  
Vol 25 (11) ◽  
pp. 2699 ◽  
Author(s):  
Alexandra Elena Stoica ◽  
Cristina Chircov ◽  
Alexandru Mihai Grumezescu
Keyword(s):  
Wound Healing ◽  
Healing Process ◽  
Contact Layer ◽  
Molecular Engineering ◽  
Wound Dressings ◽  
Wound Management ◽  
Wound Healing Process ◽  
Burn Treatment ◽  
Physiological Processes ◽  
Self Assembling

As wound healing continues to be a challenge for the medical field, wound management has become an essential factor for healthcare systems. Nanotechnology is a domain that could provide different new approaches concerning regenerative medicine. It is worth mentioning the importance of nanoparticles, which, when embedded in biomaterials, can induce specific properties that make them of interest in applications as materials for wound dressings. In the last years, nano research has taken steps to develop molecular engineering strategies for different self-assembling biocompatible nanoparticles. It is well-known that nanomaterials can improve burn treatment and also the delayed wound healing process. In this review, the first-line of bioactive nanomaterials-based dressing categories frequently applied in clinical practice, including semi-permeable films, semipermeable foam dressings, hydrogel dressings, hydrocolloid dressings, alginate dressings, non-adherent contact layer dressings, and multilayer dressings will be discussed. Additionally, this review will highlight the lack of high-quality evidence and the necessity for future advanced trials because current wound healing therapies generally fail to provide an excellent clinical outcome, either structurally or functionally. The use of nanomaterials in wound management represents a unique tool that can be specifically designed to closely reflect the underlying physiological processes in tissue repair.

scholarly journals Antioxidant biocompatible composite collagen dressing for diabetic wound healing in rat model

2021 ◽  
Vol 8 (2) ◽  
Author(s):  
Bei Qian ◽  
Jialun Li ◽  
Ke Guo ◽  
Nengqiang Guo ◽  
Aimei Zhong ◽  
...  
Keyword(s):  
Wound Healing ◽  
Inflammatory Responses ◽  
Healing Process ◽  
Diabetic Rats ◽  
Wound Healing Process ◽  
Diabetic Patients ◽  
Skin Defect ◽  
Diabetic Wound ◽  
Sod Activity ◽  
Post Surgery

Abstract Associated with persistent oxidative stress, altered inflammatory responses, poor angiogenesis and epithelization, wound healing in diabetic patients is impaired. N-acetylcysteine (NAC) is reported to resist excess reactive oxygen species (ROS) production, prompt angiogenesis and maturation of the epidermis. Studies have revealed that graphene oxide (GO) can regulate cellular behavior and form cross-links with naturally biodegradable polymers such as collagen (COL) to construct composite scaffolds. Here, we reported a COL-based implantable scaffold containing a mixture of GO capable of the sustained delivery of NAC to evaluate the wound healing in diabetic rats. The morphological, physical characteristics, biocompatibility and NAC release profile of the GO-COL-NAC (GCN) scaffold were evaluated in vitro. Wound healing studies were performed on a 20 mm dorsal full-skin defect of streptozotocin (STZ)-induced diabetic rats. The injured skin tissue was removed at the 18th day post-surgery for histological analysis and determination of glutathione peroxidase (GPx), catalase (CAT) and superoxide dismutase (SOD) activity. In diabetic rats, we confirmed that the GCN scaffold presented a beneficial effect in enhancing the wound healing process. Additionally, due to the sustained release of NAC, the scaffold may potentially induce the antioxidant defense system, upregulating the expression levels of the antioxidant enzymes in the wound tissue. The findings revealed that the antioxidant biocompatible composite collagen dressing could not only deliver NAC in situ for ROS inhibition but also promote the wound healing process. This scaffold with valuable therapy potential might enrich the approaches for surgeon in diabetic wound treatment in the future.

scholarly journals Comparative effect of turmeric (Curcuma longa) and durba (Cynodon dactylon) on the healing of surgical wounds in cattle (Bos indicus)

2018 ◽  
Vol 4 (2) ◽  
pp. 164-171
Author(s):  
Mohammad Abdullah Al Mamun ◽  
Nasrin Sultana Juyena ◽  
Md Abu Hadi Noor Ali Khan ◽  
Md Aminul Islam
Keyword(s):  
Wound Healing ◽  
Cynodon Dactylon ◽  
Curcuma Longa ◽  
Healing Process ◽  
Tissue Reaction ◽  
Wound Management ◽  
Control Group ◽  
Wound Healing Process ◽  
Herbal Products ◽  
Surgical Wounds

Herbal preparations are used traditionally in wound management to promote wound healing process. In Bangladesh, there are limited researches on the use of herbal products to assist healing process. Therefore, this experiment was carried out to exploit turmeric (Curcuma longa) and durba (Cynodon dactylon) pastes in the treatment of surgical wounds in cattle and to study their healing effect on wound sutured with horizontal mattress using nylon. A total of 36 surgical wounds were made in six cattle. Tincture benzoin was used as control group. Follow-up information was recorded from day 0 to day 21 postoperatively. Some morphological characters such as swelling area of wound, elevation of sutured line from the skin surface, width of sutured area and contraction length per week were recorded to determine the healing process of the wounds. Bacteriological and histopathological studies were also performed. All wounds were closely monitored to observe any complications such as swelling, wound dehiscence, suture abscess, local infection and exudation etc. Results showed that Tr. Benzoin seal caused marked tissue reaction in wounds in comparison to turmeric and durba paste. Turmeric paste showed splendid results in the healing of the wounds produced in cattle. Durba paste also has wound healing activity but less effective than that of turmeric. This study could help veterinarians to consider herbal products especially turmeric for healing of wounds with minimum complications. Dissemination of these findings among the farmers may prevent skin degradation caused by the postoperative complications of the wound and thus improve the health status of livestock.Asian J. Med. Biol. Res. June 2018, 4(2): 164-171

scholarly journals Single Dose of N-Acetylcysteine in Local Anesthesia Increases Expression of HIF1α, MAPK1, TGFβ1 and Growth Factors in Rat Wound Healing

2021 ◽  
Vol 22 (16) ◽  
pp. 8659
Author(s):  
Wiktor Paskal ◽  
Michał Kopka ◽  
Albert Stachura ◽  
Adriana M. Paskal ◽  
Piotr Pietruski ◽  
...  
Keyword(s):  
Wound Healing ◽  
Healing Process ◽  
Control Group ◽  
Wound Healing Process ◽  
Sprague Dawley ◽  
Regenerative Processes ◽  
Wound Site ◽  
Post Surgery ◽  
Sprague Dawley Rats ◽  
Anesthetic Solution

In this study, we aimed to investigate the influence of N-acetylcysteine (NAC) on the gene expression profile, neoangiogenesis, neutrophils and macrophages in a rat model of incisional wounds. Before creating wounds on the backs of 24 Sprague–Dawley rats, intradermal injections were made. Lidocaine–epinephrin solutions were supplemented with 0.015%, 0.03% or 0.045% solutions of NAC, or nothing (control group). Scars were harvested on the 3rd, 7th, 14th and 60th day post-surgery. We performed immunohistochemical staining in order to visualize macrophages (anti-CD68), neutrophils (anti-MPO) and newly formed blood vessels (anti-CD31). Additionally, RT-qPCR was used to measure the relative expression of 88 genes involved in the wound healing process. On the 14th day, the number of cells stained with anti-CD68 and anti-CD31 antibodies was significantly larger in the tissues treated with 0.03% NAC compared with the control. Among the selected genes, 52 were upregulated and six were downregulated at different time points. Interestingly, NAC exerted a significant effect on the expression of 45 genes 60 days after its administration. In summation, a 0.03% NAC addition to the pre-incisional anesthetic solution improves neovasculature and increases the macrophages’ concentration at the wound site on the 14th day, as well as altering the expression of numerous genes that are responsible for the regenerative processes.

scholarly journals A Wound-Healing Assay Based on Ultraviolet Light Ablation

2016 ◽  
Vol 22 (1) ◽  
pp. 36-43 ◽  
Author(s):  
Shang-Ying Wu ◽  
Yung-Shin Sun ◽  
Kuan-Chen Cheng ◽  
Kai-Yin Lo
Keyword(s):  
Wound Healing ◽  
Cell Migration ◽  
Healing Process ◽  
Cell Monolayer ◽  
Wound Healing Process ◽  
Collective Cell Migration ◽  
Wound Healing Assay ◽  
Physiological Processes ◽  
Free Region ◽  
A Cell

Collective cell migration plays important roles in many physiological processes such as embryonic development, tissue repair, and angiogenesis. A “wound” occurs when epithelial cells are lost and/or damaged due to some external factors, and collective cell migration takes place in the following wound-healing process. To study this cellular behavior, various kinds of wound-healing assays are developed. In these assays, a “wound,” or a “cell-free region,” is created in a cell monolayer mechanically, chemically, optically, or electrically. These assays are useful tools in studying the effects of certain physical or chemical stimuli on the wound-healing process. Most of these methods have disadvantages such as creating wounds of different sizes or shapes, yielding batch-to-batch variation, and damaging the coating of the cell culture surface. In this study, we used ultraviolet (UV) lights to selectively kill cells and create a wound out of a cell monolayer. A comparison between the current assay and the traditional scratch assay was made, indicating that these two methods resulted in similar wound-healing rates. The advantages of this UV-created wound-healing assay include fast and easy procedure, high throughput, and no direct contact to cells.

HUBUNGAN MOBILISASI DINI TERHADAP PENYEMBUHAN LUKA POST OPERASI SECTIO CESARIA

2019 ◽  
Vol 2 (1) ◽  
pp. 14-17
Author(s):  
Nurul Aini Siagian ◽  
Syafira Nusaibah ◽  
Andayani Boang Manalu
Keyword(s):  
Wound Healing ◽  
Healing Process ◽  
Early Mobilization ◽  
Sectio Caesarea ◽  
Wound Healing Process ◽  
P Value ◽  
Cross Sectional ◽  
Care Services ◽  
Healing Wound ◽  
Rapid Healing

Early mobilization includes factors that can affect the process of wound healing after surgery. Immediate mobilization in stages is very useful for the process of healing wounds and preventing infection and venous thrombosis. The purpose of this study was to determine whether there is a relationship between early mobilization and the process of healing wound post operative sectio caesarea at Sinar Husni General Hospital Medan Helvetia. The research design used was analytic survey with cross sectional approach. The sample in this study used the Consecutive Sampling method of data collection using a checklist sheet conducted on a sample of 19 respondents. The results of the study in this study are the majority of respondents who did early mobilization and who experienced rapid wound healing process as many as 4 people (21%) while the minority of respondents who did early mobilization and who experienced slow wound healing process were 1 person (5.3%). The majority of respondents who did not mobilize early and who experienced a slow wound healing process were 11 people (57.9%) and a minority who did not mobilize early and who experienced rapid healing as many as 4 people (21.1%). Statistical test results obtained p value = 0.046 <0.005. The conclusions of the results of this study indicate there is a relationship between early mobilization and the process of healing post operative sectio of caesarea. Suggestions The results of this study can be applied as a reference to improve nursing care services, especially in providing counseling and assistance to patients.

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