scholarly journals Diet and heat - one neuronal subset two responses

2020 ◽  
Author(s):  
Elodie Prince ◽  
Jenny Kretzschmar ◽  
Laura C. Trautenberg ◽  
Marko Brankatschk

ABSTRACTThe Insulin signal cascade is one of the best studied metabolic circuits, and shows a remarkable high molecular and functional conservation across the animal kingdom. Insulin-producing cells respond directly to nutritional cues in circulation and receive modulatory input from connected neuronal networks. Neuronal control is rapid and integrates a wide range of variables including dietary change or environmental temperature. However, despite various detailed studies that demonstrated the potential of neuronal regulation the physiological relevance of this circuit remains elusive.In Drosophila, Insulin-like peptide 7 (dIlp7)-producing neurons are wired with Insulin-producing cells. We found a dual role for this neuronal subset: a.) activated dilp7-producing neurons are required to facilitate development at high temperatures, and if confronted with calorie-rich food that represses neuronal activity b.) their product, dIlp7, regulates Insulin signalling levels. Our work shows that Insulin-producing cells not simply integrate signals from circulating nutritional cues and neuronal inputs, but switch to neuronal control in response to dietary composition.

2021 ◽  
Vol 12 ◽  
Author(s):  
Elodie Prince ◽  
Jenny Kretzschmar ◽  
Laura C. Trautenberg ◽  
Susanne Broschk ◽  
Marko Brankatschk

Cellular Insulin signaling shows a remarkable high molecular and functional conservation. Insulin-producing cells respond directly to nutritional cues in circulation and receive modulatory input from connected neuronal networks. Neuronal control integrates a wide range of variables including dietary change or environmental temperature. Although it is shown that neuronal input is sufficient to regulate Insulin-producing cells, the physiological relevance of this network remains elusive. In Drosophila melanogaster, Insulin-like peptide7-producing neurons are wired with Insulin-producing cells. We found that the former cells regulate the latter to facilitate larval development at high temperatures, and to regulate systemic Insulin signaling in adults feeding on calorie-rich food lacking dietary yeast. Our results demonstrate a role for neuronal innervation of Insulin-producing cells important for fruit flies to survive unfavorable environmental conditions.


2005 ◽  
Vol 185 (1) ◽  
pp. 19-33 ◽  
Author(s):  
Andrew W Stoker

A cornerstone of many cell-signalling events rests on reversible phosphorylation of tyrosine residues on proteins. The reversibility relies on the coordinated actions of protein tyrosine kinases and protein tyrosine phosphatases (PTPs), both of which exist as large protein families. This review focuses on the rapidly evolving field of the PTPs. We now know that rather than simply scavenging phosphotyrosine, the PTPs specifically regulate a wide range of signalling pathways. To illustrate this and to highlight current areas of agreement and contention in the field, this review will present our understanding of PTP action in selected areas and will present current knowledge surrounding the regulatory mechanisms that control PTP enzymes themselves. It will be seen that PTPs control diverse processes such as focal adhesion dynamics, cell–cell adhesion and insulin signalling, and their own actions are in turn regulated by dimerisation, phosphorylation and reversible oxidation.


Author(s):  
G. W. Bryan ◽  
P. E. Gibbs

INTRODUCTIONIntracellular calcium granules rich in phosphorus are found throughout the animal kingdom and occur in a wide range of different tissues (Simkiss, 1976; Mason & Nott, 1981). However, few examples of the natural occurrence of such granules in muscle cells have been recorded and thus the discovery of abundant granules composed of calcium phosphate within the muscle fibres of the polychaete Nephtys, described by Gibbs & Bryan (1984), is of considerable interest, particularly regarding the formation and function of these unusual sarcoplasmic inclusions.


2020 ◽  
Vol 21 (8) ◽  
pp. 2718 ◽  
Author(s):  
Yasmine Lund-Ricard ◽  
Patrick Cormier ◽  
Julia Morales ◽  
Agnès Boutet

A major challenge in medical research resides in controlling the molecular processes of tissue regeneration, as organ and structure damage are central to several human diseases. A survey of the literature reveals that mTOR (mechanistic/mammalian target of rapamycin) is involved in a wide range of regeneration mechanisms in the animal kingdom. More particularly, cellular processes such as growth, proliferation, and differentiation are controlled by mTOR. In addition, autophagy, stem cell maintenance or the newly described intermediate quiescence state, Galert, imply upstream monitoring by the mTOR pathway. In this review, we report the role of mTOR signaling in reparative regenerations in different tissues and body parts (e.g., axon, skeletal muscle, liver, epithelia, appendages, kidney, and whole-body), and highlight how the mTOR kinase can be viewed as a therapeutic target to boost organ repair. Studies in this area have focused on modulating the mTOR pathway in various animal models to elucidate its contribution to regeneration. The diversity of metazoan species used to identify the implication of this pathway might then serve applied medicine (in better understanding what is required for efficient treatments in human diseases) but also evolutionary biology. Indeed, species-specific differences in mTOR modulation can contain the keys to appreciate why certain regeneration processes have been lost or conserved in the animal kingdom.


Author(s):  
David A Farnell

Nucleotide excision repair (NER) is a vital DNA repair pathway which acts on a wide range of helix-distorting lesions. The importance of this pathway is highlighted by its functional conservation throughout evolution and by several human diseases, such as xeroderma pigmentosum, which are caused by a defective NER pathway. This review summarizes the NER mechanisms present in all three domains of life: eukaryotes, bacteria, and archaea.


1978 ◽  
Vol 40 (3) ◽  
pp. 433-438 ◽  
Author(s):  
W. H. Close

1. From the relation between metabolizable energy (me) intake and heat loss (H), energy retention (ER), protein (P) and fat (F) deposition the energy costs of maintenance (MEm) and the partial efficiencies of energy retention (k) and protein (kp) and fat (kf) retention were determined in growing pigs at environmental temperatures of 10, 15, 20, 25 or 30°.2. k decreased with increase in environmental temperature from 0.79 at 10° to 0.63 at 30° with 0.67 at the thermally-neutral temperature of 25°. Each 0.04 decrease in k was associated with a 100 kJ/kg0.75 per d decrease in mem Analysis, within several ranges of environmental temperature, suggested a curvilinear relation between ER and me intake indicating a decrease in k with increase in level of feeding, particularly at thermally-neutral temperatures.3. Both kp and kf were similar at each environmental temperature and decreased from 0.78 at 10° to 0.63 at 30°. These values are discussed in relation to those predicted from experimentation and it is suggested that the wide range of predicted estimates of kp could be attributed to differences in the rate of protein turnover.


2017 ◽  
pp. 425-439 ◽  
Author(s):  
A. KÁDKOVÁ ◽  
V. SYNYTSYA ◽  
J. KRUSEK ◽  
L. ZÍMOVÁ ◽  
V. VLACHOVÁ

Transient receptor potential A1 (TRPA1) is an excitatory ion channel that functions as a cellular sensor, detecting a wide range of proalgesic agents such as environmental irritants and endogenous products of inflammation and oxidative stress. Topical application of TRPA1 agonists produces an acute nociceptive response through peripheral release of neuropeptides, purines and other transmitters from activated sensory nerve endings. This, in turn, further regulates TRPA1 activity downstream of G-protein and phospholipase C-coupled signaling cascades. Despite the important physiological relevance of such regulation leading to nociceptor sensitization and consequent pain hypersensitivity, the specific domains through which TRPA1 undergoes post-translational modifications that affect its activation properties are yet to be determined at a molecular level. This review aims at providing an account of our current knowledge on molecular basis of regulation by neuronal inflammatory signaling pathways that converge on the TRPA1 channel protein and through modification of its specific residues influence the extent to which this channel may contribute to pain.


Author(s):  
O. V. Kovalenko

The main material of the hydrotechnical structures of the water management and reclamation complex is concrete. Its durability is the main indicator of reliability of building operation. The high density of hydraulic concrete, which determines the water resistance, is a guarantee of its durability. At the same time, in hydraulic concrete during the operation under the influence of aggressive environmental factors various defects (cracks, dissected zones)develop, which reduce its waterproofing capability. These defectsare the main centers of water filtration through building structures. At first, drip filtration develops in buildings, which in the course of time increases into active jet filtration. That can lead to the complete destruction of the building. In this connection, the issue of restoration of monolithicity and waterproofing of concrete hydro-technical constructions is relevant. The solution of the problem may be the use of injection waterproofing of the structures when using hydroactive polyurethane resins. Due to the presence in the chain of macromolecules the final isocyanate (-NCO) groups, polyurethanes are capable for interacting with water molecules with the release of carbon dioxide, which leads to foaming. As a result, water blockage occurs in the concrete body and its waterproofing is restored. The analysis of the modern construction market shows that there is currently a sufficiently wide range of single- and two-component hydroactive polyurethane resins capable of reacting with water with foam formation. As the study has shown, the rate of polymerization, the beginning and the end of polyurethane compositionsfoaming substantially depend on the rate of catalyst or polymerization accelerator in the composition, as well as on the environmental temperature. So,when increasing the rate of catalyst in the mixture from 2 to 9%, the rate of polymerization reaction increases by 3,3-3,9 times, and when increasing the environmental temperature from 10 to 25 °C, the polymerization rate increases in 1,3-1,4 times. The foaming time of polyurethane compositions varies within 0,8 ... 16 minutes. Depending on the polyurethane formulations, the resins can be slow- reactive, fast-reactive and ultrafast-reactive, which opens a prospect for their application in the conditions of the filtration of different intensity: from drip to jet. Two-component polyurethane resins are the most promising for application in the technology of injection waterproofing on hydraulic engineering structures of water management and reclamation area, which enable to eliminate water leakage through the structures of different intensity: from drip to active jet filtration. Two-component polyurethane resins such as CarboPur, Tunnelinjekt, PenePurFoam, when contacting with water, form a rigid elastic foam with high physical and mechanical properties: bending strength up to 80 MPa, adhesion to water-saturated concrete to 2, 8 MPa. Field study proved the high efficiency of injecting waterproofing with the use of hydroactive polyurethane resins in the conditions of water filtration through the structures of docking facilities of pumping stations. In the course of implementation of the projects on the restoration of waterproofing of reinforced concrete structures, the following types of work were performed: eliminating leaks through the isolation joints of concrete, as well as through shrinkage and structural cracks; eliminating leaks in the adjacent zones of "wall-floor", "wall-ceiling"; sealing leaks through the places of engineering communications laying(pipelines); arrangement of shut-off and veal waterproofing in the walls (injection of resins into dilapidated zones of the structure).


2020 ◽  
Author(s):  
Patricia G. Izquierdo ◽  
Vincent O’Connor ◽  
Christopher Green ◽  
Lindy Holden-Dye ◽  
John Tattersall

AbstractInhibition of acetylcholinesterase by either organophosphates or carbamates causes anti-cholinesterase poisoning. This arises through a wide range of neurotoxic effects triggered by the overstimulation of the cholinergic receptors at synapses and neuromuscular junctions. Without intervention, this poisoning can lead to profound toxic effects, including death, and the incomplete efficacy of the current treatments, particularly for oxime-insensitive agents, provokes the need to find better antidotes. Here we show how the non-parasitic nematode Caenorhabditis elegans offers an excellent tool for investigating the acetylcholinesterase intoxication. The C. elegans neuromuscular junctions show a high degree of molecular and functional conservation with the cholinergic transmission that operates in the autonomic, central and neuromuscular synapses in mammals. In fact, the anti-cholinesterase intoxication of the worm’s body wall neuromuscular junction has been unprecedented in understanding molecular determinants of cholinergic function in nematodes and other organisms. We extend the use of the model organism’s feeding behaviour as a tool to investigate carbamate and organophosphate mode of action. We show that inhibition of the cholinergic-dependent rhythmic pumping of the pharyngeal muscle correlates with the inhibition of the acetylcholinesterase activity caused by aldicarb, paraoxons and DFP exposure. Further, this bio-assay allows one to address oxime dependent reversal of cholinesterase inhibition in the context of whole organism recovery. Interestingly, the recovery of the pharyngeal function after such anti-cholinesterase poisoning represents a sensitive and easily quantifiable phenotype that is indicative of the spontaneous recovery or irreversible modification of the worm acetylcholinesterase after inhibition. These observations highlight the pharynx of C. elegans as a new tractable approach to explore anti-cholinesterase intoxication and recovery with the potential to resolve critical genetic determinants of these neurotoxins’ mode of action.


2021 ◽  
Vol 12 ◽  
Author(s):  
Botong Shen ◽  
Ellen Freebern ◽  
Jicai Jiang ◽  
Christian Maltecca ◽  
John B. Cole ◽  
...  

Meiotic recombination is a fundamental biological process that facilitates meiotic division and promotes genetic diversity. Recombination is phenotypically plastic and affected by both intrinsic and extrinsic factors. The effect of maternal age on recombination rates has been characterized in a wide range of species, but the effect’s direction remains inconclusive. Additionally, the characterization of temperature effects on recombination has been limited to model organisms. Here we seek to comprehensively determine the impact of genetic and environmental factors on recombination rate in dairy cattle. Using a large cattle pedigree, we identified maternal recombination events within 305,545 three-generation families. By comparing recombination rate between parents of different ages, we found a quadratic trend between maternal age and recombination rate in cattle. In contrast to either an increasing or decreasing trend in humans, cattle recombination rate decreased with maternal age until 65 months and then increased afterward. Combining recombination data with temperature information from public databases, we found a positive correlation between environmental temperature during fetal development of offspring and recombination rate in female parents. Finally, we fitted a full recombination rate model on all related factors, including genetics, maternal age, and environmental temperatures. Based on the final model, we confirmed the effect of maternal age and environmental temperature during fetal development of offspring on recombination rate with an estimated heritability of 10% (SE = 0.03) in cattle. Collectively, we characterized the maternal age and temperature effects on recombination rate and suggested the adaptation of meiotic recombination to environmental stimuli in cattle. Our results provided first-hand information regarding the plastic nature of meiotic recombination in a mammalian species.


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