scholarly journals Native SAD Phasing at Room Temperature

2021 ◽  
Author(s):  
Jack B. Greisman ◽  
Kevin M. Dalton ◽  
Candice J. Sheehan ◽  
Margaret A. Klureza ◽  
Doeke R. Hekstra
Keyword(s):  
Data Collection ◽  
Room Temperature ◽  
Model Systems ◽  
Protein Crystal ◽  
Quality Data ◽  
Conformational Ensemble ◽  
High Quality ◽  
Collection Method ◽  
Data Collection Method ◽  
Sad Phasing

Single-wavelength anomalous diffraction (SAD) is a routine method for overcoming the phase problem when solving a new macromolecular structure. This technique requires the accurate measurement of intensities to sensitively determine differences across Bijvoet pairs, making it a stringent test for the reliability of a data collection method. Although SAD experiments are commonly conducted at cryogenic temperatures to mitigate the effects of radiation damage, such temperatures can alter the conformational ensemble of the protein crystal and may impede the merging of data from multiple crystals due to non-uniform freezing. Here, we propose a data collection strategy to obtain high-quality data from room temperature samples. To illustrate the strengths of this approach we use native SAD phasing at 6.5 keV to solve four structures of three model systems at 295 K. The resulting datasets allow for automatic phasing and model building, and exhibit alternate conformations that are well-supported by the electron density. The high-redundancy data collection method demonstrated here enables the routine collection of high-quality, room-temperature diffraction to improve the study of protein conformational ensembles.

Strategi Pemasaran Untuk Meningkatkan Jumlah Kunjungan Pada Obyek Wisata Kebun Raya Dan Kebun Binatang (KRKB) Gembira Loka Di Kota Yogyakarta

2017 ◽  
Vol 22 (1) ◽  
pp. 1-10
Author(s):  
Nur Wening ◽  
Muhammad Al Hasny ◽  
Ridha Fitryana
Keyword(s):  
Qualitative Research ◽  
Data Collection ◽  
Marketing Strategy ◽  
Swot Analysis ◽  
Internal Variable ◽  
External Variable ◽  
Collection Method ◽  
Data Collection Method ◽  
A Company

This research aims to formulate marketing strategy to increase visitors of Gembira Loka Garden and Zoo (KRKB Gembira Loka) in Yogyakarta. This research is qualitative research and uses interview, observation, and documentation as data collection method. The data is analyzed by using SWOT analysis with internal and external variable identification. The internal variable shows that location is the main strength of Gembira Loka KRKB with 0.17 by value and 5 by rating. From external variable, the result of EFAS table shows that Gembira Loka KRKB has good enough chance while the thread has less result than the chance. Gembira Loka KRKB is in quadrant 1, which is the position in which a company is considered to be in a beneficial situation due to its chance and strength. In such case, the company can utilize the chance by maximizing the strength. The following strategy to go through in this condition is supporting aggressive planning.

SIMBOLISASI DALAM NOVEL SANG ALKEMIS KARYA PAULO QUELHO

2019 ◽  
Vol 7 (4) ◽  
pp. 79
Author(s):  
Endang Maruti
Keyword(s):  
Content Analysis ◽  
Data Collection ◽  
Old Age ◽  
Qualitative Approach ◽  
Data Sources ◽  
The Novel ◽  
Note Taking ◽  
Literature Study ◽  
Collection Method ◽  
Data Collection Method

The research aims to uncover the symbols in the novel The Alchemist and to gain knowledge about the moral teachings in the symbol. This research is descriptive qualitative approach. Data sources in this study are words, phrases or sentences in the novel Alchemist. Data collection method is a literature study method with note taking technique. Data were analyzed using description and content analysis methods. The results showed that the novel The Alchemist contained many symbols. These symbols include: (1) wise parents, who symbolize both negative and positive things. From his appearance, parents can symbolize something bad, but behind his old age he symbolizes a knowledge that is very much and wise; (2) stones that symbolize something hard, not easily broken, and can provide clues to something; and (3) deserts or deserts which can be interpreted as symbols of drought, aridity, unattractiveness, emptiness, despair, determination for ignorance, and also as symbols of devotion.  

sistem informasi manajemen

2019 ◽  
Author(s):  
Novi Rozalita AR
Keyword(s):  
Data Collection ◽  
Report Cards ◽  
Collection Method ◽  
Data Collection Method ◽  
Data Value ◽  
Qualitative Descriptive ◽  
Descriptive Approach

ABSTRACTInformation systems in the processing of student value using the program the computer can facilitate teachers in processing student value more quickly and effectively. So as to produce information quickly and accurately in searching the data value of students. One is the use of E-report cards in processing grades at MAN 1 Sungai Panuah. The main focus of this research is "Processing Student Value of E-Grade X Class at MAN 1 Sungai Panuah" This research was conducted at MAN 1 Sungai Panuah by using Qualitative Descriptive approach. Data collection method used is interview

Collecting High-Quality Data

2018 ◽  
Author(s):  
Mary Kay Gugerty ◽  
Dean Karlan
Keyword(s):  
Data Collection ◽  
Social Desirability ◽  
Measurement Errors ◽  
Monitoring And Evaluation ◽  
Quality Data ◽  
Social Desirability Bias ◽  
High Quality ◽  
High Quality Data ◽  
Evaluation Systems ◽  
Demand Effect

Without high-quality data, even the best-designed monitoring and evaluation systems will collapse. Chapter 7 introduces some the basics of collecting high-quality data and discusses how to address challenges that frequently arise. High-quality data must be clearly defined and have an indicator that validly and reliably measures the intended concept. The chapter then explains how to avoid common biases and measurement errors like anchoring, social desirability bias, the experimenter demand effect, unclear wording, long recall periods, and translation context. It then guides organizations on how to find indicators, test data collection instruments, manage surveys, and train staff appropriately for data collection and entry.

scholarly journals Utilising Mood Boards as an Image Elicitation Tool in Qualitative Research

2021 ◽  
pp. 136078042199348
Author(s):  
Simon Spawforth-Jones
Keyword(s):  
Qualitative Research ◽  
Data Collection ◽  
Critical Appraisal ◽  
Research Projects ◽  
Research Project ◽  
Collection Method ◽  
Visual Method ◽  
Data Collection Method ◽  
Elicitation Methods ◽  
And Gender

The use of image elicitation methods has been recognised in qualitative research for some time; however, the use of mood boards to prompt participant discussion is currently an under-researched area. This article explores the use of mood boards as a data collection method in qualitative research. Used in design disciplines mood boards allow designers to interpret and communicate complex or abstract aspects of a design brief. In this study, I utilise mood boards as being part creative visual method and part image elicitation device. The use of mood boards is explained here in the context of a research project exploring masculinity and men’s reflexivity. In this article, I consider the benefits of utilising this method in researching reflexivity and gender before offering a critical appraisal of this method and inviting others to explore how mood boards might enhance research projects involving elicitation.

A survey on prediction of diabetes using classification algorithms

2021 ◽  
Vol 2 (104) ◽  
pp. 77-84
Author(s):  
A. Khanwalkar ◽  
R. Soni
Keyword(s):  
Machine Learning ◽  
Data Collection ◽  
Learning Algorithm ◽  
Algorithm Design ◽  
Machine Learning Algorithms ◽  
Classification Algorithms ◽  
Machine Learning Algorithm ◽  
Collection Method ◽  
Data Collection Method ◽  
Diagnostic Center

Purpose: Diabetes is a chronic disease that pays for a large proportion of the nation's healthcare expenses when people with diabetes want medical care continuously. Several complications will occur if the polymer disorder is not treated and unrecognizable. The prescribed condition leads to a diagnostic center and a doctor's intention. One of the real-world subjects essential is to find the first phase of the polytechnic. In this work, basically a survey that has been analyzed in several parameters within the poly-infected disorder diagnosis. It resembles the classification algorithms of data collection that plays an important role in the data collection method. Automation of polygenic disorder analysis, as well as another machine learning algorithm. Design/methodology/approach: This paper provides extensive surveys of different analogies which have been used for the analysis of medical data, For the purpose of early detection of polygenic disorder. This paper takes into consideration methods such as J48, CART, SVMs and KNN square, this paper also conducts a formal surveying of all the studies, and provides a conclusion at the end. Findings: This surveying has been analyzed on several parameters within the poly-infected disorder diagnosis. It resembles that the classification algorithms of data collection plays an important role in the data collection method in Automation of polygenic disorder analysis, as well as another machine learning algorithm. Practical implications: This paper will help future researchers in the field of Healthcare, specifically in the domain of diabetes, to understand differences between classification algorithms. Originality/value: This paper will help in comparing machine learning algorithms by going through results and selecting the appropriate approach based on requirements.

Perspektif Pendidikan Islam Terhadap Budaya Lipas Pada Masyarakat Mandar Majene

2021 ◽  
Vol 7 (3) ◽  
pp. 213
Author(s):  
Husnah Zainuddin Maawi ◽  
Hamzah Fathani
Keyword(s):  
Data Collection ◽  
Field Study ◽  
Family Members ◽  
Positive Culture ◽  
Islamic Education ◽  
Human Society ◽  
Collection Method ◽  
Life Process ◽  
Data Collection Method ◽  
Moral Sanction

  Penelitian ini merupakan studi lapangan yang menjelaskan masalah pokok tentang budaya lipas pada masyarakat mandar majene ditinjau dari pendidikan islam. Tujuan dari penelitian ini Antara lain untuk mengetahui konsep lipas dalam masyarakat mandar majene, faktor dilakukannya lipas dan implikasi lipas dalam pendidikan islam. Metode yang digunakan dalam penelitian adalah metode pengumpulan dara yang terdiri dari observasi dan wawancara. Peneliti terjun langsung ke lapangan dan mewawancarai langsung orang-orang yang dianggap paham dan dapat dipercaya dalam masalah lipas di daerah Majene. Hasil yang diperoleh dalam penelitian ini adalah Budaya lipas masyarakat mandar majene ini muncul sebagai hukuman moral bagi anggota keluarga yang melanggar tradisi. Lipas pada masyarakat mandar majene dipengaruhi oleh beberapa faktor seperti: sipaindongang (kawin lari), Battang Bule (hamil diluar nikah), siala tania sambona (menikah dengan bukan sederajatnya), siala to poppoang (kawin dengan orang yang punya penyakit poppo’), siala to tandissang rumbu apinna (menikah dengan orang yang tidak dikenal asal usulnya). Lipas merupakan sanksi moral yang diberikan kepada anggota keluarga yang melakukan perbuatan tercela atau perbuatan yang bertentangan dengan tradisi dan jika dilihat dari pemahaman pendidikan Islam sebenarnya islam tidak pernah melarang budaya positif dalam kehidupan masyarakat. Justru Islam dapat melestarikan budaya melalui proses kehidupan yang dilandasi dengan nilai-nilai ajaran Alquran dan al-Hadis.   This research is a field study that explains the main problem of cockroach culture in the Mandar Majene community in terms of Islamic education. The purpose of this study, among others, to determine the concept of cockroach in human society, the factors of knowing cockroaches and the interaction of cockroaches in Islamic education. The method used in this research is data collection method which consists of observation and interviews. Researchers went directly to the field and directly interviewed people who were considered to be knowledgeable and trustworthy in the problem of cockroaches in the Majene area. The results obtained in this study are the cockroach culture of the Majene mandar community appears as a moral punishment for family members who violate traditions. Lipas in the Mandar Majene community is influenced by several factors such as: sipaindongang (elopement), Battang Bule (pregnant out of wedlock), siala tania sambona (married to someone who is not equal), siala to poppoang (married with someone who has Poppo'), unlucky to tandissang rumbu apinna (married to a person whose origin is unknown). Lipas is a moral sanction given to family members who commit disgraceful acts or actions that are contrary to tradition and when viewed from the understanding of Islamic education, it has never prohibited positive culture in people's lives. Islam can occur through a life process that is based on the values ​​of the teachings of the Quran and al-Hadith.

scholarly journals Radiation damage effects on protein conformation at room temperature and 100K

2014 ◽  
Vol 70 (a1) ◽  
pp. C344-C344
Author(s):  
Silvia Russi ◽  
Shawn Kann ◽  
Henry van den Bedem ◽  
Ana M. González
Keyword(s):  
Data Collection ◽  
Radiation Damage ◽  
Crystal Structures ◽  
Room Temperature ◽  
Relative Rate ◽  
Absorbed Dose ◽  
Conformational Ensemble ◽  
Data Sets ◽  
Cryogenic Temperatures ◽  
Full Data

Protein crystallography data collection at synchrotrons today is routinely carried out at cryogenic temperatures to mitigate radiation damage to the crystal. Although damage still takes place, at 100 K and below, the immobilization of free radicals increases the lifetime of the crystals by orders of magnitude. Increasingly, experiments are carried out at room temperature. The lack of adequate cryo-protectants, the induced lattice changes or internal disorders during the cooling process, and the convenience of collecting data directly from the crystallization plates, are some of the reasons. Moreover, recent studies have shown that flash-freezing affects the conformational ensemble of crystal structures [1], and can hide important functional mechanisms from observation [2]. While there has been a considerable amount of effort in studying radiation damage at cryo-temperatures, its effects at room temperature are still not well understood. We investigated the effects of data collection temperature on secondary local damage to the side chain and main chain from different proteins. Data were collected from crystals of thaumatin and lysozyme at 100 K and room temperature. To carefully control the total absorbed dose, full data sets at room temperature were assembled from a few diffraction images per crystal. Several data sets were collected at increasing levels of absorbed dose. Our analysis shows that while at cryogenic temperatures, radiation damage increases the conformational variability, _x0004_at room temperature it has the opposite effect_x0005_. We also observed that disulfide bonds appear to break up at a different relative rate at room temperature, perhaps because of a more active repair mechanism. Our analysis suggests that elevated conformational heterogeneity in crystal structures at room temperature is observed despite radiation damage, and not as a result thereof.

scholarly journals Measuring social networks in primates: wearable sensors versus direct observations

2020 ◽  
Vol 476 (2236) ◽  
pp. 20190737
Author(s):  
Valeria Gelardi ◽  
Jeanne Godard ◽  
Dany Paleressompoulle ◽  
Nicolas Claidiere ◽  
Alain Barrat
Keyword(s):  
Data Collection ◽  
Wearable Sensors ◽  
A Priori ◽  
Population Level ◽  
Sensor Data ◽  
Collection Method ◽  
Data Collection Method ◽  
The Social ◽  
Different Types ◽  
Data Yield

Network analysis represents a valuable and flexible framework to understand the structure of individual interactions at the population level in animal societies. The versatility of network representations is moreover suited to different types of datasets describing these interactions. However, depending on the data collection method, different pictures of the social bonds between individuals could a priori emerge. Understanding how the data collection method influences the description of the social structure of a group is thus essential to assess the reliability of social studies based on different types of data. This is however rarely feasible, especially for animal groups, where data collection is often challenging. Here, we address this issue by comparing datasets of interactions between primates collected through two different methods: behavioural observations and wearable proximity sensors. We show that, although many directly observed interactions are not detected by the sensors, the global pictures obtained when aggregating the data to build interaction networks turn out to be remarkably similar. Moreover, sensor data yield a reliable social network over short time scales and can be used for long-term studies, showing their important potential for detailed studies of the evolution of animal social groups.

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