Efficacy of Vibrio anguillarum antigen administered by intraperitoneal injection route in Japanese flounder, Paralichthys olivaceus (Temminck et Schlegel)

2005 ◽  
Vol 36 (11) ◽  
pp. 1104-1111 ◽  
Author(s):  
Jian Li ◽  
Dongmei Gao ◽  
Qun Wang ◽  
Jiye Wang ◽  
Qingyin Wang
Keyword(s):  
Intraperitoneal Injection ◽  
Paralichthys Olivaceus ◽  
Vibrio Anguillarum ◽  
Japanese Flounder

scholarly journals Impact of Ringer’s Solution Challenge Stress to Immunostimulatory Experiment, Insights From Japanese Flounder

2020 ◽  
Vol 11 ◽  
Author(s):  
Jinxiang Liu ◽  
Zan Li ◽  
Yujue Wang ◽  
Quanqi Zhang
Keyword(s):  
Immune Response ◽  
Intraperitoneal Injection ◽  
Expression Profiles ◽  
Paralichthys Olivaceus ◽  
Metabolic Process ◽  
Japanese Flounder ◽  
Innate Immune ◽  
Fundamental Information ◽  
Immune Related Genes ◽  
The Impact

Ringer’s or phosphate buffer saline (PBS) solution buffer usually was used as dilution butter in intraperitoneal injection. Stress could activate immune response, inflammatory response and glycogen metabolic process. The impact of solution buffer as a stressor to immune system was ignored in immunostimulatory experiment. In this report, we tested the hypothesis that the innate immune response and glycogen metabolic process were altered when it were challenged with Ringer’s in Japanese flounder (Paralichthys olivaceus). RNA-seq was performed after challenge with Ringer’s at 8 h and 48 h. The data revealed that the expression profiles of blood, gill, and kidney were significantly changed. Differentially expressed genes (DEGs) were identified, and energy metabolic and immune-related genes were up-regulated or down-regulated obviously. GO and KEGG analyses showed that DEGs were mainly enriched in innate immune terms and pathways. Weighted gene co-expression networks analysis (WGCNA) also indicated the highest association module with stress. A total of 16 genes were detected in the gray module, which were immune-related and metabolic-related genes. These results provided fundamental information on intraperitoneal injection with solution buffer. It offered useful clues to further explore the functional mechanism of stress and immunity.

scholarly journals Systematic Identification and Analysis of Circular RNAs of Japanese Flounder (Paralichthys olivaceus) in Response to Vibrio anguillarum Infection

Genes ◽  
2021 ◽  
Vol 12 (1) ◽  
pp. 100
Author(s):  
Xianhui Ning ◽  
Li Sun
Keyword(s):  
Paralichthys Olivaceus ◽  
Expression Patterns ◽  
Vibrio Anguillarum ◽  
Circular Rna ◽  
Japanese Flounder ◽  
Immune Defense ◽  
Circular Rnas ◽  
Pathogen Infection ◽  
North Asia ◽  
Time Specific

Circular RNA (circRNA) is a new class of non-coding RNA that is structured into a closed loop without polyadenylation. Recent studies showed that circRNAs are involved in the host immune response to pathogen infection. Japanese flounder (Paralichthys olivaceus), an important economical marine fish cultured in north Asia, is affected by Vibrio anguillarum, a pathogenic bacterium that can infect a large number of fish. In this study, we systematically explored the circRNAs in the spleen of V. anguillarum-infected flounder at different infection time points. A total of 6581 circRNAs were identified, 148 of which showed differential expression patterns after V. anguillarum infection and were named DEcirs. Most of the DEcirs were strongly time-specific. The parental genes of the DEcirs were identified and functionally classified into diverse pathways, including immune-related pathways. Among the immune-related DEcirs, seven were predicted to sponge 18 targeted miRNAs that were differentially expressed during V. anguillarum infection (named DETmiRs). Further analysis showed that the DEcirs and their corresponding DETmiRs intertwined into complicated immune related networks. These results indicate that in flounder, circRNAs are regulated by V. anguillarum and form interactive networks with mRNAs and miRNAs that likely play important roles in the immune defense against pathogen infection.

Protection of Japanese flounder (Paralichthys olivaceus) against Vibrio anguillarum with a DNA vaccine containing the mutated zinc-metalloprotease gene

Vaccine ◽  
2009 ◽  
Vol 27 (15) ◽  
pp. 2150-2155 ◽  
Author(s):  
Hui Yang ◽  
Jixiang Chen ◽  
Guanpin Yang ◽  
Xiao-Hua Zhang ◽  
Rui Liu ◽  
...  
Keyword(s):  
Dna Vaccine ◽  
Paralichthys Olivaceus ◽  
Vibrio Anguillarum ◽  
Japanese Flounder ◽  
Zinc Metalloprotease

scholarly journals Micro-Transcriptome Analysis Reveals Immune-Related MicroRNA Regulatory Networks of Paralichthys olivaceus Induced by Vibrio anguillarum Infection

2020 ◽  
Vol 21 (12) ◽  
pp. 4252
Author(s):  
Xianhui Ning ◽  
Li Sun
Keyword(s):  
Immune Response ◽  
Transcriptome Analysis ◽  
Regulatory Networks ◽  
Expression Profiles ◽  
Paralichthys Olivaceus ◽  
Vibrio Anguillarum ◽  
Japanese Flounder ◽  
Integrated Analysis ◽  
Immune Gene ◽  
Hub Genes

MicroRNAs (miRNAs) are non-coding regulatory RNAs that play a vital part in the host immune response to pathogen infection. Japanese flounder (Paralichthys olivaceus) is an important aquaculture fish species that has suffered from bacterial diseases, including that caused by Vibrio anguillarum infection. In a previous study, we examined the messenger RNA (mRNA) expression profiles of flounder during V. anguillarum infection and identified 26 hub genes in the flounder immune response. In this study, we performed the micro-transcriptome analysis of flounder spleen in response to V. anguillarum infection at 3 different time points. Approximately 277 million reads were obtained, from which 1218 miRNAs were identified, including 740 known miRNAs and 478 novel miRNAs. Among the miRNAs, 206 were differentially expressed miRNAs (DEmiRs), and 104 of the 206 DEmiRs are novel miRNAs identified for the first time. Most of the DEmiRs were strongly time-dependent. A total of 1355 putative target genes of the DEmiRs (named DETGs) were identified based on integrated analysis of miRNA-mRNA expressions. The DETGs were enriched in multiple functional categories associated with immunity. Thirteen key DEmiRs and 66 immune DETGs formed an intricate regulatory network constituting 106 pairs of miRNAs and DETGs that span five immune pathways. Furthermore, seven of the previously identified hub genes were found to be targeted by 73 DEmiRs, and together they formed interlinking regulatory networks. These results indicate that V. anguillarum infection induces complicated miRNA response with extensive influences on immune gene expression in Japanese flounder.

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