The variation in eruption of permanent teeth and loss of deciduous teeth in Western Australian children

: Most conventional treatments for neurodegenerative diseases fail due to their focus on neuroprotection rather than neurorestoration. Stem cell‐based therapies are becoming a potential treatment option for neurodegenerative diseases as they can home in, engraft, differentiate and produce factors for CNS recovery. Stem cells derived from human dental pulp tissue differ from other sources of mesenchymal stem cells due to their embryonic neural crest origin and neurotrophic property. These include both dental pulp stem cells [DPSCs] from dental pulp tissues of human permanent teeth and stem cells from human exfoliated deciduous teeth [SHED]. SHED offer many advantages over other types of MSCs such as good proliferative potential, minimal invasive procurement, neuronal differentiation and neurotrophic capacity, and negligible ethical concerns. The therapeutic potential of SHED is attributed to the paracrine action of extracellularly released secreted factors, specifically the secretome, of which exosomes is a key component. SHED and its conditioned media can be effective in neurodegeneration through multiple mechanisms, including cell replacement, paracrine effects, angiogenesis, synaptogenesis, immunomodulation, and apoptosis inhibition, and SHED exosomes offer an ideal refined bed-to-bench formulation in neurodegenerative disorders. However, in spite of these advantages, there are still some limitations of SHED exosome therapy, such as the effectiveness of long-term storage of SHED and their exosomes, the development of a robust GMP-grade manufacturing protocol, optimization of the route of administration, and evaluation of the efficacy and safety in humans. In this review, we have addressed the isolation, collection and properties of SHED along with its therapeutic potential on in vitro and in vivo neuronal disorder models as evident from the published literature.

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Comparative evaluation of osteogenic differentiation potential of stem cells derived from dental pulp and exfoliated deciduous teeth cultured over granular hydroxyapatite based scaffold

BMC Oral Health ◽

10.1186/s12903-021-01621-0 ◽

2021 ◽

Vol 21 (1) ◽

Author(s):

Manal Nabil Hagar ◽

Farinawati Yazid ◽

Nur Atmaliya Luchman ◽

Shahrul Hisham Zainal Ariffin ◽

Rohaya Megat Abdul Wahab

Keyword(s):

Stem Cells ◽

Osteogenic Differentiation ◽

Dental Pulp ◽

3D Culture ◽

Permanent Teeth ◽

Deciduous Teeth ◽

Osteogenic Potential ◽

Control Group ◽

Rt Pcr ◽

Differentiation Potential

Abstract Background Mesenchymal stem cells isolated from the dental pulp of primary and permanent teeth can be differentiated into different cell types including osteoblasts. This study was conducted to compare the morphology and osteogenic potential of stem cells from exfoliated deciduous teeth (SHED) and dental pulp stem cells (DPSC) in granular hydroxyapatite scaffold (gHA). Preosteoblast cells (MC3T3-E1) were used as a control group. Methodology The expression of stemness markers for DPSC and SHED was evaluated using reverse transcriptase-polymerase chain reaction (RT-PCR). Alkaline phosphatase assay was used to compare the osteoblastic differentiation of these cells (2D culture). Then, cells were seeded on the scaffold and incubated for 21 days. Morphology assessment using field emission scanning electron microscopy (FESEM) was done while osteogenic differentiation was detected using ALP assay (3D culture). Results The morphology of cells was mononucleated, fibroblast-like shaped cells with extended cytoplasmic projection. In RT-PCR study, DPSC and SHED expressed GAPDH, CD73, CD105, and CD146 while negatively expressed CD11b, CD34 and CD45. FESEM results showed that by day 21, dental stem cells have a round like morphology which is the morphology of osteoblast as compared to day 7. The osteogenic potential using ALP assay was significantly increased (p < 0.01) in SHED as compared to DPSC and MC3T3-E1 in 2D and 3D cultures. Conclusion gHA scaffold is an optimal scaffold as it induced osteogenesis in vitro. Besides, SHED had the highest osteogenic potential making them a preferred candidate for tissue engineering in comparison with DPSC.

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Observational Study Regarding the Relationship between Nutritional Status, Dental Caries, Mutans Streptococci, and Lactobacillus Bacterial Colonies

International Journal of Environmental Research and Public Health ◽

10.3390/ijerph18073551 ◽

2021 ◽

Vol 18 (7) ◽

pp. 3551

Author(s):

Eugen Silviu Bud ◽

Cristina Ioana Bica ◽

Oana Elena Stoica ◽

Alexandru Vlasa ◽

Daniela Eșian ◽

...

Keyword(s):

Dental Caries ◽

Observational Study ◽

Nutritional Status ◽

Positive Association ◽

Mutans Streptococci ◽

Permanent Teeth ◽

Deciduous Teeth ◽

Dmft Index ◽

The Relationship ◽

Significant Health

The prevalence of dental caries and obesity is high as both raise significant health problems. The objective of this study was to evaluate the relationship between dental caries, the number of salivary colonies forming units of Mutans Streptococci (MS) and Lactobacillus (LB), and the nutritional status in a group of children from Transylvania. This observational study used a sample of 154 school children, aged 9 to 12 years. The prevalence of caries was measured using the decayed, missing, and filled teeth index for deciduous teeth (dmft index) and for permanent teeth (DMFT index). Height and weight were assessed for each subject, and their body mass index (BMI) percentile was calculated. Salivary levels of Mutans Streptococci (MS) and Lactobacillus (LB) were determined using the CRT Bacteria Test from Ivoclar Vivadent. In our study, we found a positive association between the BMI percentile, MS count, LB count, tooth brushing frequency, and the incidence of dental caries in children aged 9 to 12 years old. Future preventive programs should include nutrition control in order to prevent both the apparition of dental caries and obesity in children.

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Tetracycline syrups and children’s teeth

Drug and Therapeutics Bulletin ◽

10.1136/dtb.22.14.55 ◽

1984 ◽

Vol 22 (14) ◽

pp. 55-56

Keyword(s):

At Risk ◽

In Utero ◽

Permanent Teeth ◽

Deciduous Teeth ◽

Enamel Hypoplasia ◽

Yellow Colour ◽

Paediatric Patients ◽

Short Course

It has been known for over 25 years that treatment with oral tetracyclines can permanently stain children’s teeth1 yet these drugs are still needlessly being prescribed for children. In 1982 over 75,000 prescriptions for a liquid tetracycline preparation were dispensed, most of which were probably for children; up to one third of paediatric patients have been affected,2–6 although the proportion has fallen over the last 10 years.7 Even a short course of tetracycline can stain both the permanent3,8 and deciduous teeth9 a disfiguring greyish-brown or yellow colour. Children are at risk from the 14th week in utero, when calcification of deciduous teeth begins, to their 7th year when calcification of the permanent teeth is complete. Whether tetracyclines produce enamel hypoplasia10 or promote caries11 is disputed.12

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Space Maintainer Treatment In Premature Loss Deciduous Tooth

SONDE (Sound of Dentistry) ◽

10.28932/sod.v5i1.2325 ◽

2020 ◽

Vol 5 (1) ◽

pp. 1-12

Author(s):

Rey Bintang Pamungkas ◽

Jeffrey .

Keyword(s):

Child Development ◽

Clinical Examination ◽

Growth And Development ◽

Development Process ◽

Deciduous Tooth ◽

Permanent Teeth ◽

Deciduous Teeth ◽

Space Maintainer

Abstract. Introduction: The deciduous tooth period is an important period in child development. Premature loss of deciduous teeth is defined as the loss of deciduous teeth before they approach the eruption of permanent teeth. The prevalance of premature loss offered in several studies obtained was between 4.3% and 42.6%. Method: An 10 years-old-boy accompanied by her mother came to RSGMP Unjani, complaints of lower right nack teeth missing because they have to extracted since 3 mouth ago. Tooth have been extracted because of caries. Result: Result of clinical examination was found premature loss teeth 75, 74, and 85. The space in Moyers analysis was +0.2 mm in region 3 and +0.1 mm in region 4. Patient was treated with a fungsional removable-space-maintainer on teeth 75, 74, and 85. Conclusion: The results of premature loss is excess space in the arch, so to prevent further occlusion abnormalities in child’s growth and development process, we need a special appliance that is used to protect the space due to premature loss of deciduous teeth. Keywords: Premature loss, space maintainer, deciduous teeth

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A Rare Tooth Anomaly (Double Teeth) in Deciduous Teeth: A Case Report

Journal of Isfahan Dental School ◽

10.18502/ijds.v17i3.7538 ◽

2021 ◽

Author(s):

Fataneh Ghorbanyjavadpour

Keyword(s):

Case Report ◽

Lateral Incisor ◽

Permanent Teeth ◽

Deciduous Teeth ◽

Tooth Size ◽

Genetic Characteristics ◽

Size And Shape ◽

Size Discrepancy ◽

Tooth Size Discrepancy ◽

Double Teeth

Introduction: The Size and shape of the teeth are genetic characteristics. Anomaly in tooth size and shape is due to disturbances in a sequence of morph differentiation and his to differentiation in tooth bud formation periods and is more common in permanent dentition than deciduous teeth. The most common tooth size discrepancy is in the upper lateral incisor and upper and lowers 2nd premolar teeth. About 5% of malocclusions are due to tooth size discrepancy. For having the best occlusion, we must have the appropriate size and shape of teeth. As there is a relation between deciduous and permanent teeth, proper evaluation and timely intervention are essential for achieving a good occlusion in anterior and posterior segments of permanent teeth. Thus we need interceptive orthodontic in mixed dentition period with a good treatment plan for removing the malformed tooth at the proper time and appliance therapy for eruption guidance of other teeth. Case Report: A rare tooth anomaly (double teeth) in deciduous teeth: The case reported here is a 9- years old boy with a delayed eruption of the right lower permanent lateral incisor and gemination of lower right deciduous canine and crowded upper incisors that refer to the orthodontic department of dental school of Ahvaz Jundishapur University of Medical Sciences on7th October of 2020. Conclusion: After creating patient’s file, the further steps of molding from the patient and gathering all diagnostic records such as panoramic radiography, intraoral photography was carried out and ordered to remove the malformed baby tooth afterward. In the next stage, on the patient’s treatment, we installed a strap onto the patient’s first permanent molars, remolded them, then created a lingual arch to maintain the vegetative space in the lower permanent canine and prevent its collapse, and increased the patient’s overbite.

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Taxa de sucesso de restaurações com resina composta bulk-fill: Revisão de literatura / Success rate of bulk-fill composite restorations: Literature review

ID on line REVISTA DE PSICOLOGIA ◽

10.14295/idonline.v13i47.2027 ◽

2019 ◽

Vol 13 (47) ◽

pp. 397-409

Author(s):

Atyla Freitas Soares ◽

Ana Clara De Sá Pinto

Keyword(s):

Clinical Trials ◽

Survival Rate ◽

Literature Review ◽

Success Rate ◽

Composite Resin ◽

Clinical Excellence ◽

Google Scholar ◽

Permanent Teeth ◽

Deciduous Teeth ◽

De Se

Desde a introdução das resinas compostas na Odontologia, foram evidenciadas muitas melhorias em suas propriedades. No entanto, estes materiais apresentam limitações, como a contração de polimerização, que pode resultar em fissuras de esmalte, defeito marginal, formação de fenda e microinfiltração. Para superar estas deficiências, algumas estratégias práticas incluem o uso de técnicas de inserção incremental das resinas. Porém, a mesma é demorada quando utilizada para preencher cavidades grandes e volumosas em dentes posteriores, o que tornou desejável o desenvolvimento de uma alternativa a este procedimento, e assim surgiram as resinas compostas do tipo bulk-fill, que podem ser inseridas em incrementos de 4 a 5 mm de espessura devido à sua maior translucidez. Diante deste contexto, o objetivo do presente estudo foi apresentar as taxas de sucesso das resinas bulk-fill em restaurações de dentes permanentes e decíduos, comparando ainda o resultado do seu uso em relação às resinas convencionais. A pesquisa bibliográfica foi realizada online, utilizando a ferramenta de busca Google Scholar e outras seis bases de dado eletrônicas, que são o Pubmed, Web of Sciences, National Institute for Health and Clinical Excellence, Clinical Trials–US National Institute of Health e Lilacs, sem restrição de data da publicação. Foram utilizadas as palavras-chave: “composite resin; bulk-fill composite resin; survival rate; deciduous teeth; permanent teeth”. Desta forma, foram encontrados 273 artigos que, excluindo-se os revisão de literatura, cartas ao editor e editoriais, foram reduzidos para 22 estudos a serem revisados. A lista de artigos obtida foi analisada e os artigos foram selecionados baseados nos seus títulos e resumos, de forma que foram incluídos artigos publicados em português, inglês e espanhol. Foi possível observar que há uma grande semelhança entre as resinas bulk-fill e as resinas compostas convencionais no que diz respeito às taxas de sucesso. No entanto, sugere-se que sejam realizados estudos com metodologias e amostras similares, a fim de se confirmar os resultados obtidos no presente estudo.

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