ANALYSIS OF BIOACTIVE COMPOUNDS AND MORPHOLOGICAL TRAITS IN INDONESIAN RODENT TUBER MUTANT CLONES OF PEKALONGAN ACCESSION USING GC-MS

2018 ◽  
Vol 80 (2) ◽  
Author(s):  
Ragapadmi Purnamaningsih ◽  
Nesti Fronika Sianipar
Keyword(s):  
Genetic Diversity ◽  
Herbal Medicine ◽  
Gamma Irradiation ◽  
Bioactive Compounds ◽  
Morphological Traits ◽  
Octadecadienoic Acid ◽  
Acid Ethyl Ester ◽  
Hexadecanoic Acid ◽  
Effective Technique ◽  
Considerable Potential

Rodent tuber (Typhonium flagelliforme Lodd.) is one of the considerable potential Indonesian medicinal plants. There are many origins of rodent tuber plants in Indonesia such as Pekalongan. Increasing genetic diversity of rodent tuber’s Pekalongan accession had been done by using gamma irradiation. Its produced 23 clones of the 7th generation of mutant plants. The focus of this study was on the analysis of biomass and bioactive compounds from those clones using GC-MS. The aim of this study was to determine new bioactive compounds which affect as anticancer in the 7th mutant generation of rodent tuber plant Pekalongan accession. The result showed that 8 mutant clones of rodent tuber had higher biomass than the control. 10 bioactive compounds were detected in the leaves from 7 mutant clones (20-1-1-2-2-3, 20-1-2-2-1-3, 20-1-2-1-2-2, 20-1-2-4-5, 20-1-1-1-3, 20-4-2-1-1-1, 20-1-2-1-2-6) and 7 bioactive compounds in the tubers from 9 mutant clones (20-1-1-2-2-3, 20-1-2-2-1-3, 20-1-2-1-2-2, 20-1-2-4-5, 20-1-1-1-3, 20-4-2-1-1-1, 20-1-2-1-2-6, 20-1-2-2-4-3, 20-1-2-2-3). The bioactive compounds in the mutant clones was 4 times greater than the control. Phytol isomer and eicosane were found as new bioactive compounds in the leaves. 5 new bioactive compounds were found in the tubers for example Hexadecanoic acid ethyl ester, octadecadienoic acid, squalene, beta, eicosane and octacosane. Gamma irradiation is an effective technique to increase the huge numbers of bioactive compounds which can be applied as anticancer, antitumor and antimicobial herbal medicine.

BIOACTIVE COMPOUNDS IN ETHANOL EXTRACT OF LENTINUS SQUARROSULUS MONT - A NIGERIAN MEDICINAL MACROFUNGUS

2018 ◽  
Vol 15 (2) ◽  
pp. 42 ◽  
Author(s):  
Morenike Olutunmbi Adeoye-Isijola ◽  
Olufunmiso Olusola Olajuyigbe ◽  
Segun Gbolagade Jonathan ◽  
Roger Murugas Coopoosamy
Keyword(s):  
Ethyl Ester ◽  
Bioactive Compounds ◽  
Octadecadienoic Acid ◽  
Biological Activities ◽  
Ethyl Oleate ◽  
Acid Ethyl Ester ◽  
Ethyl Esters ◽  
Gas Chromatography Mass Spectrometry ◽  
Bioactive Components ◽  
Hexadecanoic Acid

Background: The continuous search for new lead compounds of therapeutic importance has become necessary in the face of treatment failures and multidrug resistance plaguing the world. While many plants and higher fungi are sources of bioactive compounds yet to be fully harnessed, understanding the bioactive components in macrofungus could serve as a lead for investigating its biological activities and medicinal potentials. Materials and Methods: The bioactive compounds in the ethanolic extract of Lentinus Squarrosulus, an edible Nigerian macrofungus, were investigated by Gas Chromatography-Mass Spectrometry (GC-MS) analysis. Results: There were nine bioactive compounds in this edible macrofungus. Of these compounds, 9,12-Octadecanoic acid ethyl ester (37.39%; RT:39.815) was the highest in quantity, followed by Hexadecanoic acid ethyl ester (14.49%; RT:36.550). Other fatty acids, their ethyl esters and other compounds identified included 2-Butenethioic acid,3-(ethylthio)-S-(1-methylethyl) ester (4.51%; RT:15.866), n-Hexadecanoic acid (4.74%; RT:36.034), 9,12-Octadecadienoic acid (Z,Z)- (11.88%; RT:39.429), 9,17-Octadecadienal,(Z)- (5.01%; RT:39.500), ethyl oleate (5.27%; RT:39.898), 3a,6-Methano-3aH-indene,2,3,6,7 tetrahydro (4.04%; RT:48.379), and 9,12-Octadecadienoic acid (Z,Z)-,2 hydroxy-1-(hydroxymethyl) ethyl ester (12.68%; RT:48.682). Some of these compounds have antimicrobial, antioxidant, hepatoprotective, hypocholesterolemic as well as cancer preventive activities amongst others. Conclusion: This study showed the bioactive components of therapeutic potentials in L. squarrosulus while creating a platform for screening, isolating and identifying many bioactive components which may be useful in the treatment of the various ailments, disorders and diseases in the nearest future.

scholarly journals Gas Chromatography-Mass Spectrometry (GC-MS) Analysis and Phytochemical Screening of Polyherbal Aqueous Leaves Extract (PALE)

2021 ◽  
pp. 10-18
Author(s):  
M. Idu ◽  
M. O. Aihiokhai ◽  
C. A. Imoni ◽  
C. E. Akokigho ◽  
N. C. Olali
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Octadecadienoic Acid ◽  
Octadecenoic Acid ◽  
Acid Ethyl Ester ◽  
Gas Chromatography Mass Spectrometry ◽  
Phytochemical Screening ◽  
Hexadecanoic Acid ◽  
Bioactive Constituents ◽  
Ms Analysis

Background: Polyherbal plant extracts which usually comprise of two or more plant parts often contain a wide array of key phytoactive constituents relevant in attaining greater therapeutic efficacy. The active constituents derived from individual plants are insufficient to provide attractive pharmacological action when compared to a combination of multiple herbs. Objective: To conduct phytochemical screening of polyherbal aqueous leaf extracts                            (PALE) and analysis of compounds present in it by gas chromatography-mass spectrometry (GC-MS). Materials and Methods: The polyherbal extract was prepared from the combined aqueous extracts of leaves of Alchornea cordifolia, Sorghum bicolor and Pennisetum glaucum using ratio 1:1:1 w/v. Phytochemical screening was done via standard analytical methods. The identification and characterization of compounds by GC-MS analysis was performed on gas chromatography system coupled with mass spectrometry. Results: The phytochemical analysis of PALE revealed the presence of phenols, saponins, flavonoids, alkaloids and tannins in varying quantities. GC-MS analysis of the extract depicts the presence of key bioactive compounds. Thirty-two bioactive compounds were identified with various retention time and % peaks. The major compounds identified in terms of % peak area are n-Hexadecanoic acid (6.72), Hexadecanoic acid, ethyl ester (7.28), 9,12-Octadecadienoic acid (16.54) and 9-Octadecenoic acid ethyl ester (12.92). Disulfide, dimethyl (0.04), 2-Methoxy-4-vinylphenol (0.28), 1-Dodecanol (0.85), 10-Phenyldecanoic acid (0.12), 1-Hexadecanol (0.75), Methoxyacetic acid, pentadecyl ester (0.27), 9-Octadecenoic acid (Z)-, phenylmethyl ester (0.16), Hexadecanoic acid, 2-hydroxy-1-(hydroxymethyl) (1.09), were among the minor compounds identified in the extract. From the study, 9,12-Octadecadienoic acid indicated the highest peak with a retention time of 20.556 minutes and % peak area of 16.54%. Conclusion: The presence of the revealed bioactive constituents in PALE may suggest its nutraceutical, pharmacological and therapeutic relevance. Therefore, in view of the medicinal importance associated with the observed bioactive constituents, further studies on the toxicity level of  the extract is advised subsequently.

scholarly journals GC-MS Analysis of the Rauwolfia vomitoria Ethanol Extracts

2021 ◽  
pp. 34-45
Author(s):  
I. I. Asoro ◽  
O. A. T. Ebuehi ◽  
M. N. Igwo-Ezikpe
Keyword(s):  
Ethyl Ester ◽  
Bioactive Compounds ◽  
Vaccenic Acid ◽  
Octadecenoic Acid ◽  
Acid Ethyl Ester ◽  
Hexadecanoic Acid ◽  
Beneficial Effects ◽  
Ms Analysis ◽  
Pharmaceutical Industries ◽  
Ethanol Extracts

Bioactive compounds are the frontline potent agents in both nutraceuticals and pharmaceutical industries. The bioactive compounds are gaining much importance for their ability in enhancing resistance to various diseases and to improve the health of people both by traditional and modern ways of administrations. R. vomitoria is one of the medicinal plants used traditionally to manage hypertension, diabetes and mental disorder. This present study sought to characterize the bioactive components of R. vomitoria leaf and root ethanol extracts using Gas-Chromatography-Mass Spectrophotometry (GC-MS). The results of the GC-MS analysis provide different peaks indicating the presence of 22 phytochemicals in the plant leaf and 16 phytochemicals in the root. The major bioactive compounds in the leaf were squalene (18.69%), phytol (16.47%), n-hexadecanoic acid (15.68%), 7-tetradecenal, (Z) (12.90%), 9,12,15-octadecatrienoic acid, ethyl ester, (Z,Z)-, (9.56%) and others, while the roots contains; cis-vaccenic acid (32.13%), n-hexadecanoic acid (15.41%), (E)-9-octadecenoic acid ethyl ester (9.83), cyclohexanecarbonitrile 1-(-4- chlorophenyl (9.45%), 8H-azeceno[5,4-b] indol-8-one, 5-ethylidene (7.66%) and other minor compounds. Pharmacological activities of these compounds indicated that the compounds present in the leaf of the plant can be used as a crude drug which could be developed into a novel drug. Some of these compounds have antimicrobial, antioxidant, hepatoprotective, hypocholesterolemic as well as cancer preventive activities amongst others. The findings suggest that there is an indication that both R. vomitoria leaves and roots contain potent bioactive compounds that may be linked to its beneficial effects on health, with the leaf taking the lead. It is therefore recommended as a plant of phytopharmaceutical significance.

scholarly journals GC-MS ANALYSIS OF BIOACTIVE COMPOUNDS IN ETHANOL LEAVES EXTRACT OF SPHENOCENTRUM JOLLYANUM AND THEIR BIOLOGICAL ACTIVITIES.

2022 ◽  
Vol 06 (01) ◽  
Author(s):  
Emmanuel Uka1,
Keyword(s):  
Molecular Weight ◽  
Bioactive Compounds ◽  
Biological Activities ◽  
Octadecenoic Acid ◽  
Acid Ethyl Ester ◽  
Molecular Formula ◽  
Hexadecanoic Acid ◽  
Total Extract ◽  
Medicinal Uses ◽  
Ms Analysis

Sphenocentrum jollyanum is a plant genus of the family Menispermaceae. It has high medicinal importance as it is used traditionally to treat various diseases such as jaundice, breast engorgement related to the menstrual cycle, tumour, fibroids and improve the health of people. The present investigation was carried out to analyze the bioactive compounds present in ethanol crude extract of Sphenocentrum jollyanum leaves using GC-MS analysis. GC-MS analysis of ethanol extract Sphenocentrum jollyanum was done using a 7890A GC system (Agilent Technologies), coupled with 5977B MSD (Agilent Technologies) while the mass spectra of the compounds found in the extract was matched with the National Institute of Standards and Technology (NIST) library. A total of 45 bioactive compounds representing 99.98% of the total extract based on the retention time, peak area, molecular formula, molecular weight, and biological activities were identified by GC-MS which ranges from high molecular weight to low molecular weight compounds. The major compounds identified with their peak area percentages were 2,4-Di-tertbutylphenol, (21.05%), Z-8-Methyl-9-tetradecenoic acid (19.12), Hexadecanoic acid, ethyl ester (7.86%), Diisooctyl phthalate (7.13%), Phytol, Oleic Acid (7.03), 6,9,12- Octadecatrien-1-ol (6.65%), 3-Eicosene, (E)-(4.63%), 2-Methyl-Z, Z-3,13-octadecadienol (4.24%), n- Hexadecanoic acid (4.09%), trans-13-Octadecenoic acid (3.81%), Cyclohexene, 4-(4-ethylcyclohexyl) -1- pentyl- (3.74%), Dibutyl phthalate (3.20%), and 9-Oxabicyclo (6.1.0) nonane, cis-(3.18%). The presence of these major phytoconstituents in the leaf extract provides various biological activities including antifungal, antibacterial, antioxidant, anti-inflammatory, and anti-tumour which supports the ethno-medicinal uses of the plant in curing diseases. We recommend

scholarly journals Evaluation of the bioactive compounds of Vernonia amygdalina Delile extracts and their antibacterial potentials on water-related bacteria

2021 ◽  
Vol 45 (1) ◽  
Author(s):  
Olubukola Olusola-Makinde ◽  
Olayinka Bukola Olabanji ◽  
Tope Abraham Ibisanmi
Keyword(s):  
Ethyl Ester ◽  
Bioactive Compounds ◽  
Aqueous Extract ◽  
Acid Ethyl Ester ◽  
Ethanol Extract ◽  
Bacterial Isolates ◽  
Hexadecanoic Acid ◽  
Vernonia Amygdalina ◽  
Leaf Extracts ◽  
Microbiological Test

Abstract Background This study focused on the evaluation of the bioactive compounds of Vernonia amygdalina Del. leaf extracts and their antibacterial potential on some water-related bacterial isolates. The bacterial isolates were confirmed using standard microbiological test. The leaves of V. amygdalina were subjected to extraction using the Maceration method with water and ethanol as the extraction solvents. Gas chromatography–mass spectroscopy (GC–MS) was carried out on extracts. Antibacterial susceptibility test of V. amygdalina extracts on isolates was carried out. Results Aqueous extract of V. amygdalina had a higher percentage yield (11.89%) than the ethanol extract (5.37%). The GC–MS carried out revealed the presence of butanoic acid, squalene, palmitaldehyde, octadecanoic acid, Z-hexadecanoic acid ethyl ester, oxirane, tetradecyl, 3- methyl-2-phenylindole, n-heneicosane, phytol, methyl-2-O-benzyl-d-arabinofuranoside, cholest-5-en-3-ol acetate; with hexadecanoic acid ethyl ester and 1,1-diethoxy-3methylbutane having the highest percentage composition of 24.37% and 13.42% in aqueous and ethanol extract, respectively, aqueous extract highly inhibited Escherichia coli with an inhibition zone of 10.333 ± 0.882 and 36.667 ± 0.882 for 25 mg/ml and 100 mg/ml, respectively, while the ethanol extracts inhibited most of the isolates with an inhibition range of 7.000 ± 1.155 to 30.333 ± 0.882. The minimum inhibitory concentration for both extracts on the isolates varies from 25 to 50 mg/ml. Conclusions The ethanol extract of V. amygdalina had a higher inhibitory activity on the bacterial isolates than water. These findings indicate the potential of ethanol extract of V. amygdalina leaf in the treatment of water borne infections.

scholarly journals Fatty acids and their esters from Cicuta virosa L.

2014 ◽  
Vol 14 ◽  
pp. 71-74
Author(s):  
U Jargalsaikhan ◽  
S Javzan ◽  
D Selenge ◽  
D Nedelcheva ◽  
S Philipov ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Linoleic Acid ◽  
Ethyl Ester ◽  
Octadecadienoic Acid ◽  
Acid Ethyl Ester ◽  
Hexadecanoic Acid ◽  
Unsaturated Esters ◽  
Aerial Parts ◽  
First Time

n-Hexane and chloroform fractions of aerial parts and roots of Cicuta virosa L. were investigated by GC-MS. As a result of the study 25 fatty acids and their esters have been identified. Two unsaturated esters such as linoleic acid ethyl ester (IX, 16.66%), and n- hexadecanoic acid ethyl ester (VII, 10.12%), the fatty acid n-hexadecanioc acid (VI, 8.10%) made up the bulk of the aerial parts. Four unsaturated esters such as linoleic acid ethyl ester (IX, 10.15%), dibutylphthalate (XII, 9.55%), n-hexadecanoic acid ethyl ester (VII, 8.19%) and 9, 12, 15 - octadecatrienoic acid ethyl ester (X, 5.9%), two fatty acids as n-hexadecanoic acid (VI, 8,15%) and 9,12-octadecadienoic acid (VIII, 4,5%) predominated in the roots of Cicuta virosa L. These known fatty acids and their esters were found for the first time in this plant species. DOI: http://dx.doi.org/10.5564/mjc.v14i0.203 Mongolian Journal of Chemistry 14 (40), 2013, p71-74

PROFILING OF GUAVA GENOTYPES GROWN IN VIDARBHA CONDITIONS ON THE BASIS OF MORPHOLOGICAL TRAITS

2017 ◽  
Vol 23 (1) ◽  
Author(s):  
R.A. PATIL ◽  
S.G. BHARAD ◽  
S.N. SAWANT
Keyword(s):  
Genetic Diversity ◽  
Genetic Variability ◽  
Morphological Traits ◽  
Considerable Extent ◽  
Fruit Traits ◽  
Breeding Programmes ◽  
Tree Leaf ◽  
Qualitative Characteristics ◽  
Intrapopulation Diversity

Assessment of genetic diversity in the available germplasm is the prerequisite for development of improved genotypes through planned breeding programmes. In the view of this Forty-eight genotypes of seedling origin guava along with 1 check (L-49/Sardar) collected and conserved at germplasm block, Main Garden, Department of Horticulture, Dr. P. D. A. University, Akola were evaluated for genetic variability and diversity based on the qualitative characteristics. The genotypes were evaluated for sixteen morphological traitsviz. tree, leaf, floral and fruit traits. Results Show considerable extent of variability amongst the 49 genotypes in each traits. A sizeable amount of intrapopulation diversity recorded can be used to identify diverse parents which can be utilized in hybridization programmes.

Medicinal Plants and Bioactive Compounds for Diabetes Management: Important Advances for Drug Discovery

2020 ◽  
Vol 26 ◽  
Author(s):  
Kondeti Ramudu Shanmugam ◽  
Bhasha Shanmugam ◽  
Gangigunta Venkatasubbaiah ◽  
Sahukari Ravi ◽  
Kesireddy Sathyavelu Reddy
Keyword(s):  
Herbal Medicine ◽  
Medicinal Plants ◽  
Bioactive Compounds ◽  
Diabetes Management ◽  
Therapeutic Potential ◽  
Public Health Problem ◽  
In Vivo Studies ◽  
Major Public Health Problem

Background : Diabetes is a major public health problem in the world. It affects each and every part of the human body and also leads to organ failure. Hence, great progress made in the field of herbal medicine and diabetic research. Objectives: Our review will focus on the effect of bioactive compounds of medicinal plants which are used to treat diabetes in India and other countries. Methods: Information regarding diabetes, oxidative stress, medicinal plants and bioactive compounds were collected from different search engines like Science direct, Springer, Wiley online library, Taylor and francis, Bentham Science, Pubmed and Google scholar. Data was analyzed and summarized in the review. Results and Conclusion: Anti-diabetic drugs that are in use have many side effects on vital organs like heart, liver, kidney and brain. There is an urgent need for alternative medicine to treat diabetes and their disorders. In India and other countries herbal medicine was used to treat diabetes. Many herbal plants have antidiabetic effects. The plants like ginger, phyllanthus, curcumin, aswagandha, aloe, hibiscus and curcuma showed significant anti-hyperglycemic activities in experimental models and humans. The bioactive compounds like Allicin, azadirachtin, cajanin, curcumin, querceitin, gingerol possesses anti-diabetic, antioxidant and other pharmacological properties. This review focuses on the role of bioactive compounds of medicinal plants in prevention and management of diabetes. Conclusion: Moreover, our review suggests that bioactive compounds have the potential therapeutic potential against diabetes. However, further in vitro and in vivo studies are needed to validate these findings.

Synthesis and Biological Activity Study of Tanshinone Derivatives: A Literature and Patent Review

2020 ◽  
Vol 20 (28) ◽  
pp. 2520-2534
Author(s):  
He Huang ◽  
Chuanjun Song ◽  
Junbiao Chang
Keyword(s):  
Biological Activity ◽  
Herbal Medicine ◽  
Chemical Synthesis ◽  
Bioactive Compounds ◽  
Chinese Herbal Medicine ◽  
Salvia Miltiorrhiza ◽  
Salvia Miltiorrhiza Bunge ◽  
Chinese Herbal ◽  
Patent Review

: Tanshinones are a class of bioactive compounds present in the Chinese herbal medicine Danshen (Salvia miltiorrhiza Bunge), containing among others, abietane diterpene quinone scaffolds. Chemical synthesis and biological activity studies of natural and unnatural tanshinone derivatives have been reviewed in this article.

scholarly journals GC-MS ANALYSIS OF INVASIVE AQUATIC WEED, PISTIA STRATIOTES L. AND EICHHORNIA CRASSIPES (MART.) SOLMS

2017 ◽  
Vol 9 (3) ◽  
pp. 111 ◽  
Author(s):  
Tulika Tyagi ◽  
Mala Agarwal
Keyword(s):  
Methyl Ester ◽  
Ethyl Ester ◽  
Eichhornia Crassipes ◽  
Acid Methyl Ester ◽  
Acid Ethyl Ester ◽  
Pistia Stratiotes ◽  
Aquatic Weed ◽  
Hexadecenoic Acid ◽  
Hexadecanoic Acid ◽  
Acid Methyl

Objective: To investigate the bioactive components of an invasive aquatic weed, Pistia stratiotes L. and Eichhornia crassipes (Mart.) Solms vegetative parts by using Gas Chromatography-Mass Spectrometer (GC-MS).Methods: The chemical compositions of the ethanol extract of whole plant Pistia stratiotes L. and Eichhornia crassipes (Mart.) Solms was investigated using Agilent Technologies GC-MS (GC-7890A, MS 5975C).Results: The results of GC-MS analysis of the ethanolic extract revealed the existence of 28 phytochemical compounds in Pistia stratiotes L. n-Hexadecanoic acid,-11-Hexadecenoic acid, ethyl ester, Hexadecanoic acid, ethyl ester, Octadecanoic acid, ethyl ester, 2-Cyclopenten-1-one, 5-hydroxy-2,3-dimethyl, L-Glutamine, 2-Pentadecanone, 6,10,14-trimethyl, Linolelaidic acid, methyl ester, 9,12,15-Octadecatrienoic acid, methyl ester,(Z,Z,Z), Nonadecane, 12,15-Octadecadiynoic acid, methyl ester, Hexadecanoic acid, 2-hydroxy-1-(hydroxymethyl)ethyl ester, Diisooctyl phthalate, Docosanoic acid, ethyl ester, Stigmasterol, Bis(2-ethylhexyl) phthalate, 1-Monolinoleoylglycerol trimethylsilyl ether, Ethyl iso-allocholate are the major compound.The ethanolic extract of Eichhornia crassipes (Mart.) Solms contains 43 phytochemical compounds of high and low molecular weight n-Hexadecanoic acid, E-11-Hexadecenoic acid, ethyl ester, Palmitic acid, Phytol, 9,12,15-Octadecatrienal, 9,12-Octadecadienoic acid, ethyl ester, Linolenic acid, ethyl ester, Stearic acid, ethyl ester, Hexadecanoic acid, 2-hydroxy-1-(hydroxymethyl)ethyl ester, α-Glyceryl linolenate, 1-Monolinoleoylglycerol trimethylsilyl ether, Linoleic acid, 2,3-bis-(O-TMS)-propyl ester, Stigmasterol, Linolelaidic acid, methyl ester, 9,12,15-Octadecatrienoic acid, ethyl ester, (Z,Z,Z), Ethyl iso-allocholate, Cholesta-22,24-dien-5-ol, 4,4-dimethyl are the major compounds.These results indicates Pistia stratiotes L. and Eichhornia crassipes (Mart.) Solms possess potent antioxidant, anti-inflammatory, anticancer, antitumour, antiarthritic, cancer preventive, antibacterial effects so can be recommended as a plant of phytopharmaceutical importance.Conclusion: The ethanol extract of Pistia stratiotes L. and Eichhornia crassipes (Mart.) Solms proves as a potential source of bioactive compounds of pharmacological importance.

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