scholarly journals Seed germination analysis in germplasm of capsicum annum

2017 ◽  
Vol 12 (3) ◽  
Author(s):  
Alexander Ray Rayiappan ◽  
Zulaikha Sarobo ◽  
Muhammad Arshad Javed
Keyword(s):  
Seed Germination ◽  
Room Temperature ◽  
Daily Life ◽  
Germination Percentage ◽  
Peak Period ◽  
Mean Values ◽  
Capsicum Annum ◽  
Significant Difference ◽  
Varietal Response ◽  
Germination Traits

Capsicum annum is commercially cultivated due to its nutritional and pharmaceutical values. It is particularly important in Malaysia due to its abundant use in daily life food. Although it is warm seasonal plant, its productivity is not high in tropical environment. High seed germination is the prerequisite for good seedling establishment. Present study is conducted to study the varietal response for germination traits in diverse capsicum germplasm.  Five germination traits were focused e.g.  Final germination percentage (FGP), speed of germination (SG), initiation of germination (IG), days to 50% germination (R50) and peak period of germination (GP). Seeds of each variety were kept in triplicate at room temperature using Yoshida medium coupled with photoperiod of 14 hours for two weeks. Mean values for FGP (81%), SG (rate=2), IG (9th day) and R50 (10th day) and GP (12th day) were observed in 93 varieties. Analysis of variance (ANOVA) showed significant difference for all germination traits, reflecting that present germplasm is diverse for germination traits. These findings would be useful to improve the germination traits in capsicum hybridization programs.

scholarly journals Enhancement of rice and wheat seed germination and seedling vigor by biocontrol agent, Cladosporium cladosporioides

2020 ◽  
Vol 7 (1) ◽  
pp. 9-15
Author(s):  
Md Touhidul Islam
Keyword(s):  
Seed Germination ◽  
Biocontrol Agent ◽  
Germination Percentage ◽  
Cladosporium Cladosporioides ◽  
Germination Index ◽  
Rice Seed ◽  
Seedling Vigor ◽  
Wheat Seed ◽  
Mean Values ◽  
Vigor Index

The biocontrol agent, Cladosporium cladosporioides, strain BOU1 was used to evaluate the rice and wheat plant growth by germination percentage, germination index, vigor index- I and vigor index-II in rice and wheat seed. All the variables of germination and seedling vigor of rice and wheat were increased by the conidial suspension of C. cladosporioides. The rice seed treated with C. cladosporioides had the highest germination index and vigor index-I with average mean values of 8.2 and 4.5, respectively; while the treated wheat seed showed the highest germination percentage and vigor index- II with average mean values of 80.3 and 0.05, respectively. The research is concluded that the biocontrol agent C. cladosporioides improves seed germination and seedling vigor of wheat and rice, which can finally be converted into superior yield even in adverse conditions. Res. Agric., Livest. Fish.7(1): 09-15, April 2020

scholarly journals Seed and Seedling Response of Irvingia gabonensis (Aubry-Lecomte ex O’Rorke) Baill. to Lengths of Storage at Room Temperature and Pretreatments

2020 ◽  
pp. 33-42
Author(s):  
Olufunke O. Olayode
Keyword(s):  
Room Temperature ◽  
Block Design ◽  
Germination Percentage ◽  
Significant Loss ◽  
Hot Water ◽  
State University ◽  
Assessment Period ◽  
Significant Difference ◽  
Natural Range ◽  
Irvingia Gabonensis

Aims: This study was carried out to evaluate the response of seeds and seedlings of Irvingia gabonensis to different lengths of storage at room temperature and pretreatments. Study Design:  Randomized Complete Block Design was used. Place and Duration of Study: The experiment was carried out at the nursery of the Department of Forest Resources and Wildlife Management, Ekiti State University, Ado-Ekiti, Nigeria. Methods: Mature fruits of I. gabonensis were obtained from its area of natural range in Ekiti State, Nigeria. Depulped seeds were subjected to different lengths of storage and pretreatments. The different lengths of storage were Fresh, 7days, 14days, 21days and 28days represented as LS1, LS2, LS3, LS4 and LS5 respectively. Pretreatments were control, steeping in water at room temperature, soaking in hot water and scarification represented as PT1, PT2, PT3 and PT4 respectively. Results: In LS1, germination began under PT1, PT2 and PT4 at 20, 19 and 21 Days after Sowing (DAS) respectively producing 90%, 100% and 100% germination respectively. Under LS2, seeds subjected to PT1, PT2 and PT4 germinated at 23, 24 and 23 DAS giving 90%, 90% and 80% germination respectively. At LS3, germination began in PT1, PT2 and PT4 at 26, 27 and 26 DAS producing 60%, 90% and 60% germination. However, seeds subjected to PT3 did not germinate throughout duration of experiment. Although, Analysis of Variance revealed significant difference at certain assessment period for seedling growth variables, there were no marked differences. Conclusion: Seeds of Irvingia gabonensis can be successfully stored for up to four weeks at room temperature without a significant loss in viability although germination percentage would reduce with increasing length of storage. Soaking I. gabonensis seeds in water at room temperature can be adopted for pretreating its seeds prior to sowing although untreated seeds still produced good germination.

scholarly journals Embryo Development of Cypripedium formosanum in Relation to Seed Germination In Vitro

2005 ◽  
Vol 130 (5) ◽  
pp. 747-753 ◽  
Author(s):  
Yung-I Lee ◽  
Nean Lee ◽  
Edward C. Yeung ◽  
Mei-Chu Chung
Keyword(s):  
Seed Germination ◽  
Embryo Development ◽  
Developmental Stages ◽  
Culture Media ◽  
Nile Red ◽  
Germination Percentage ◽  
Zygotic Embryogenesis ◽  
Seed Collection ◽  
Significant Difference

This investigation documents the key anatomical features in embryo development of Cypripedium formosanum Hayata, in association with the ability of embryos to germinate in vitro, and examines the effects of culture media and seed pretreatments on seed germination. A better understanding of zygotic embryogenesis for the Cypripedium L. species would provide insights into subsequent germination events and aid in the in vitro propagation of these endangered species. In seeds collected at 60 days after pollination (DAP), soon after fertilization, no germination was recorded. The best overall germination was found at 90 DAP (≈70%), at which time early globular to globular embryos with a single-celled suspensors can be observed. After 135 DAP, the seeds germinated poorly. At this time the inner integument shrinks and forms a tight layer, which encloses the embryo, the so-called “carapace.” Using Nile red stain, a cuticular substance was detected in the carapace, which may play a role in the impermeability of the mature seed and may help the seeds survive in the stringent environment. At maturity (after 210 DAP), the embryo proper has an average size of eight cells along its length and six cells across the width. Lipids and proteins are the main storage products within the embryo. To improve seed germination, experiments were conducted to test the suitability of various media and pretreatments of seeds. When different media were used, except for the Harvais medium at 120 DAP, there was no significant difference in seed germination at three different developmental stages tested. Soaking mature seeds in 1% NaOCl or treating them with ultrasound may slightly increase the germination percentage. For seed germination, our results indicate that the timing of seed collection outweighs the composition of medium and the seed pretreatments.

scholarly journals Differential Responses of Dimorphic Seeds and Seedlings to Abiotic Stresses in the Halophyte Suaeda salsa

2021 ◽  
Vol 12 ◽  
Author(s):  
Hao Zhang ◽  
Mingfang Hu ◽  
Hongyuan Ma ◽  
Li Jiang ◽  
Zhenyong Zhao ◽  
...  
Keyword(s):  
Seed Germination ◽  
Seedling Growth ◽  
Abiotic Stresses ◽  
Inhibitory Effect ◽  
Germination Percentage ◽  
Suaeda Salsa ◽  
High Germination ◽  
Germination Characteristics ◽  
Black Seeds ◽  
Germination Traits

The period between seed germination and seedling establishment is one of the most vulnerable stages in the life cycle of annuals in the saline environments. Although germination characteristics of Suaeda salsa seeds have been reported, the comparative germination patterns of dimorphic seeds and seedling growth to different abiotic stresses remain poorly understood. In this study, germination responses of dimorphic seeds to light and temperature were compared. Meanwhile, responses of dimorphic seeds and thereafter seedlings of S. salsa to different concentrations of NaCl and Na2SO4 were also tested. The results showed that the light did not significantly affect germination percentage of brown seeds, but significantly promoted germination of black seeds. Brown seeds could reach high germination percentage over a wide temperature range, however, germination of black seeds gradually increased with the increase of temperature. Brown seeds had higher germination percentage and velocity than black seeds under the same salt conditions. However, black seeds had higher recovery germination than brown seeds when transferred to deionized water. Young seedlings had lower salt tolerance than germinating seeds. At the same concentrations, Na2SO4 had stronger inhibitory effect on seed germination and seedling growth than NaCl. This study comprehensively compared germination traits of dimorphic seeds and seedling growth of S. salsa, and then developed a conceptual model to explain their adaptation to harsh saline environment.

scholarly journals SEED GERMINATION AND SEEDLING ESTABLISHMENT OF CHERIMOYA (ANNONA CHERIMOLA MILL.) AT DIFFERENT TEMPERATURES

2020 ◽  
Vol 53 (2) ◽  
pp. 185-194
Author(s):  
A. KHELOUFI ◽  
L. M. MANSOURI ◽  
H. KHETTACHE
Keyword(s):  
Seed Germination ◽  
Room Temperature ◽  
Germination Percentage ◽  
Annona Cherimola ◽  
Seedling Length ◽  
Different Temperatures ◽  
External Conditions ◽  
Mean Germination Time ◽  
Coefficient Of Velocity ◽  
Kinetics Of

Cherimoya (Annona cherimola Mill.) has an exceptional flavor and aroma, which makes it a fruit with great potential. However, little is known about its propagation by seeds. According to the scientific literature, the germination of cherimoya seeds is affected much more by external conditions than by internal conditions. Germination of cherimoya variety ‘Concha Lisa’ were tested for germination at constant temperatures of 25, 30, 40°C, and at room temperature, varying from 20-25°C, coupled with total darkness. Seeds were sown in Petri dishes (0.8% agar water), for 25 days of incubation. The kinetics of germination was determined according to five closely related parameters, viz. final germination percentage (FGP), mean germination time (MGT), coefficient of velocity of germination (CVG), time to 50% germination (T50) and seedling length (SL). The temperature of 30°C was found optimally suitable with 70.8% FGP, 17.5 days MGT and 3.91 cm SL, while the room temperature of 20-25°C slightly improved germination with only 25% FGP. Furthermore, significant decrease in FGP and SL was observed at 25°C and 40°C of temperature in comparison to 30°C. The analysis also revealed that cherimoya seed germination, day 10-15 after seed sowing is suitable for final counts. An overview on the emergence of cherimoya seedlings, during a 12-week period in pots is presented.

scholarly journals Effect of Nitrogen Addition on Selection of Germination Trait in an Alpine Meadow on the Tibet Plateau

2021 ◽  
Vol 12 ◽  
Author(s):  
Kun Liu ◽  
Yang Liu ◽  
Zhilong Zhang ◽  
Shiting Zhang ◽  
Carol C. Baskin ◽  
...  
Keyword(s):  
Seed Germination ◽  
Alpine Meadow ◽  
Germination Rate ◽  
Nitrogen Addition ◽  
Germination Percentage ◽  
N Addition ◽  
Percentage Germination ◽  
Alpine Meadows ◽  
Environmental Filter ◽  
Germination Traits

Seed germination requirements may determine the kinds of habitat in which plants can survive. We tested the hypothesis that nitrogen (N) addition can change seed germination trait-environmental filter interactions and ultimately redistribute seed germination traits in alpine meadows. We determined the role of N addition on germination trait selection in an alpine meadow after N addition by combining a 3-year N addition experiment in an alpine meadow and laboratory germination experiments. At the species level, germination percentage, germination rate (speed) and breadth of temperature niche for germination (BTN) were positively related to survival of a species in the fertilized community. In addition, community-weighted means of germination percentage, germination rate, germination response to alternating temperature and BTN increased. However, germination response to wet-cold storage (cold stratification) and functional richness of germination traits was lower in alpine meadows with high-nitrogen addition than in those with no, low and medium N addition. Thus, N addition had a significant influence on environmental filter-germination trait interactions and generated a different set of germination traits in the alpine meadow. Further, the effect of N addition on germination trait selection by environmental filters was amount-dependent. Low and medium levels of N addition had less effect on redistribution of germination traits than the high level.

scholarly journals Effect of pre-sowing treatment on seed germination of Ixora coccinea L.

2017 ◽  
Vol 8 ◽  
Author(s):  
Amruta Shivajirao Shinde ◽  
Niranjana Sunil Chavan
Keyword(s):  
Water Treatment ◽  
Seed Germination ◽  
Analysis Of Variance ◽  
Cold Water ◽  
Germination Percentage ◽  
Hot Water ◽  
Hot Water Treatment ◽  
Significant Difference ◽  
Coastal Plant ◽  
Ixora Coccinea

<p><em>Ixora coccinea</em> L. is ornamentally and medicinally important coastal plant also associated with mangroves of Sindhudurg and Ratnagiri district. In present study seeds of <em>I. coccinea</em> were treated with cold water, 50°C  hot water, germinator solution and also 100 ppm, 200 ppm, 500 ppm GA. Germination percentage were measured every day until no germination were observed. Data was analysed using analysis of variance (ANOVA) and significant mean difference in pair of treatments was analysed by using t-Test. Seeds of <em>I. coccinea </em>treated with 100 ppm GA showed highest germination percentage i.e. 90% followed by hot water treated seeds and it  showed 85%  germination.<em> </em>Seeds were treated with GA, Hot water, Germinator, soaking in cold water showed significant difference in between treatments ( p&lt;0.05). Overall results suggest that 100 ppm GA and hot water treatment used for enhance the germination of <em>I. coccinea</em> seeds.</p>

scholarly journals Effect of storage conditions and sodium hypochlorite treatment on germination of Cucumis prophetarum (Cucurbitaceae) seeds from arid Arabian deserts

2021 ◽  
Author(s):  
Hatem Ahmed Shabana ◽  
Tamer Mahmoud ◽  
Sanjay Gairola ◽  
Asma Al Ketbi ◽  
Mariam Aljasmi ◽  
...  
Keyword(s):  
Seed Germination ◽  
United Arab Emirates ◽  
Sodium Hypochlorite ◽  
Room Temperature ◽  
Seedling Emergence ◽  
Seed Storage ◽  
Storage Conditions ◽  
Germination Percentage ◽  
Continuous Darkness ◽  
Temperature Requirements

Abstract Background This is the first study on seed germination of Cucumis prophetarum in the Arabian Peninsula. Light and temperature as the main environmental factors and seed storage conditions greatly affect the germination of many Cucurbitaceae species. C. prophetarum is the perennial prostrate with woody rootstocks that grow throughout the year in the arid Arabian deserts. We examined the effects of seed storage conditions and sterilization by sodium hypochlorite (NaOCL) on germination of C. prophetarum seeds. Matured, fresh and field stored seeds were collected in March of 2016 and 2017 from a population in the United Arab Emirates. Fresh and the stored seeds (field, freeze, and room temperature) were germinated at three temperature regimes (15/25, 20/30 and 25/35°C) in both continuous darkness and alternating 12 h light/12 h darkness. Results The fresh and freeze stored seeds had higher germination percentage than the field and room temperature stored seeds. High germination was recorded in both lower and moderate temperatures (15/25 and 20/30°C) in light conditions. Sterilization of the seeds by NaOCl had a negative effect on the speed and germination percentage under all storage conditions, and it changes light and temperature requirements of seeds for germination. Conclusions Germination of C. prophetarum is sensitive to incubation light and temperature as well as to the seed storage conditions. The germination ability of stored seeds indicates that seed storage behaviour of C. prophetarum is orthodox. Light and temperature requirements for seed germination reflects a suitable place, habitat, and time for seedling emergence of C. prophetarum. The effect of NaOCl treatment on the germination may be associated with concentration and exposure periods of seeds. Therefore, we suggest that when using NaOCl for seed sterilization or to stimulate the germination, caution must be taken for using its concentration and exposure time, especially for species with thin and soft seed coats like C. prophetarum. Further studies on seed germination ecology would help to understand better the adaptive strategy of the C. prophetarum in the arid desert environments.

scholarly journals Assessment of Certain Plant Species degrading Total Petroleum Hydrocarbons in Contaminated Soil

2018 ◽  
Vol 1 (1) ◽  
pp. 69-82
Author(s):  
Shahrzad Khatibi ◽  
Hossein Mirseyed Hosseini
Keyword(s):  
Seed Germination ◽  
White Clover ◽  
Petroleum Hydrocarbons ◽  
Germination Rate ◽  
Cost Effective ◽  
Germination Percentage ◽  
Total Petroleum Hydrocarbons ◽  
Environment Friendly ◽  
Significant Difference ◽  
Uncontaminated Soil

Biological techniques, especially phytoremediation, have long been recognized as cost-effective and environment friendly to eliminate pollutants from soil. This article is based on a study conducted to assess the capability of alfalfa, ryegrass and white clover to remove total petroleum hydrocarbons (TPHs) from soil. The presence of petroleum contamination significantly decreased germination percentage and rate along with biomass of alfalfa and white clover compared to uncontaminated soil. With regards to ryegrass, there was no significant difference in seed germination percentage and biomass, although the presence of petroleum decreased seed germination rate. The results indicated that these plants had effect on TPHs remediation; and removal of TPH from soil was directly related to density levels and time. Therefore, alfalfa and ryegrass in their highest density levels reduced the maximum concentration of TPHs at the end of the experiment by almost 64.41% and 60.36%, respectively, whereas only slight changes were observed in non-vegetated soil.

scholarly journals Evaluation of the Germination Potential of Citrus Seeds during the Harvesting Season

HortScience ◽  
2013 ◽  
Vol 48 (9) ◽  
pp. 1197-1199 ◽  
Author(s):  
Vladimir Orbović ◽  
Manjul Dutt ◽  
Jude W. Grosser
Keyword(s):  
Seed Germination ◽  
Room Temperature ◽  
Seed Quality ◽  
Sweet Orange ◽  
Seed Viability ◽  
Germination Percentage ◽  
Seed Moisture Content ◽  
Seed Moisture ◽  
Citrus Seeds ◽  
Time Of Harvest

The effects of fruit age on the seed quality and germination percentage of ‘Duncan’ and ‘Flame’ grapefruit and ‘Hamlin’ sweet orange were investigated. Our results suggested that seed germination varied from 98% to 100% for the two grapefruit cultivars and 85% to 100% for ‘Hamlin’ regardless of time of harvest. Within the first 5 months of the harvest season, chilling of ‘Duncan’ and ‘Hamlin’ seeds at 4 °C for 7 days after fruit sampling resulted in a lower germination percentage only with the ‘Hamlin’ seeds. Seed moisture content of all three cultivars varied slightly through the season and remained steady at 60% and 70% for batches of fresh seeds stored at room temperature or at 4 °C. Our results suggest that high seed viability and germination percentage can allow the use of these seeds for experimentation regardless of the time the fruit were picked during the harvest season.

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