Bio-Morphing of Progressive Pathologies in Haversian Cortical Bone

2012 ◽  
Vol 79 (2) ◽  
Author(s):  
É. Budyn ◽  
J. Jonvaux ◽  
T. Hoc
Keyword(s):  
Cortical Bone ◽  
Volume Fraction ◽  
Morphological Changes ◽  
Local Strain ◽  
Haversian Canal ◽  
Canal Diameter ◽  
Microstructural Model ◽  
Growth Laws ◽  
Composite Microstructure ◽  
Haversian Canals

We present an evolutionary microstructural model to study the mechanical behavior of pathological Haversian cortical bone in the framework of linear elasticity. The Haversian cortical bone includes Haversian canals, osteons, cement lines, and interstitial bone. The composite microstructure is built using a Monte Carlo (MC) algorithm and initially displays a healthy morphology, which then evolves to mimic bone progressive aging, due to osteoporosis or low remodeling. The MC algorithm incorporates bone macroscopic morphological components such as porosity and osteonal volume fraction, microscopic parameters such as osteonal and Haversian canal diameter distributions, and also pathological growth laws characteristic of aging diseases. The local mechanical properties are measured by nanoindentation and microextensometry. The microstructures are discretised by a finite element 3D model to calculate the evolving representative volume element of aging bone, the macroscopic elastic bulk properties and microscopic strain and stress fields. The macroscopic anisotropy and local strain of aging bone are compared to those of healthy tissue in order to understand how morphological changes affect bone failure.

Bovine Cortical Bone Stiffness and Local Strain are Affected by Mineralization and Morphology

2011 ◽  
Vol 79 (1) ◽  
Author(s):  
É. Budyn ◽  
J. Jonvaux ◽  
C. Funfschilling ◽  
T. Hoc
Keyword(s):  
Cortical Bone ◽  
Volume Fraction ◽  
Three Dimensional ◽  
Local Stress ◽  
Local Strain ◽  
Monte Carlo Algorithm ◽  
Young’S Moduli ◽  
Frame Work ◽  
Mechanical Factors ◽  
Haversian Canals

A multiscale analysis of the mechanical behavior of bovine Haversian cortical bone is presented in the frame-work of linear elasticity. Cortical bone displays a complex microstructure that includes four phases: Haversian canals, osteons, cement lines, and interstitial bone. Based on close experimental observations, a Monte Carlo algorithm is implemented to build the natural bone composite microstructure. To represent the hierarchical nature of bone, the algorithm incorporates macroscopic morphological components, such as its porosity and osteonal volume fraction, as well as microscopic parameters, such as the characterized distributions of the osteons diameters. Bone local mechanical properties are measured by nanoindentation and microextensometry. The three-dimensional microstructures are discretized by a finite element method in order to evaluate the representative volume element of bovine cortical bone. The numerical model calculates the macroscopic bulk and material Young’s moduli and describes the local stress and strain. How geometrical or mechanical factors affect bone failure is investigated through a comparison of the macroscopic anisotropy and local strain to experimental data.

scholarly journals Effect of Age on Bone Structure Parameters in Laying Hens

Animals ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 570
Author(s):  
Masayoshi Yamada ◽  
Chongxiao Chen ◽  
Toshie Sugiyama ◽  
Woo Kyun Kim
Keyword(s):  
Cortical Bone ◽  
Bone Quality ◽  
Bone Mineral ◽  
Volume Fraction ◽  
Bone Structure ◽  
Bone Volume ◽  
Egg Laying ◽  
Medullary Bone ◽  
Mineral Density ◽  
Tibial Diaphysis

Changes in medullary and cortical bone structure with age remain unclear. Twenty Hy-Line W36 hens, 25 or 52 weeks of age, were euthanized, and both tibiae were collected when an egg was present in the magnum. Serial cross sections of the tibiae were stained with Alcian blue. The bones were scanned using micro-computed tomography. Trabecular width (Tb.Wi) was significantly higher (p < 0.05) in 25-week-old hens, whereas medullary bone tissue volume (TV) was significantly higher (p < 0.01) in 52-week-old hens. 25-week-old hens had significantly higher (p < 0.01) bone volume fraction (BVF = calcified tissue / TV). Moreover, the cortical bone parameters were significantly higher (TV and bone mineral content (BMC) at p < 0.05, and bone volume (BV) and BVF at p < 0.01) in younger hens. Open porosity and total porosity, which indicate less density, were significantly higher (p < 0.01) in older hens. Older hens showed significantly higher (p < 0.01) tibial diaphysis TV than younger hens. Younger hens had significantly higher (p < 0.01) BV, BVF and bone mineral density (BMD) of the tibial diaphysis. These findings reveal that reductions in medullary bone quality might be associated with age-related low estrogen levels and stimulation of osteoclastic bone resorption by parathyroid hormone. Cortical bone quality decreased with enlargement of the Haversian canals and loss of volume, with a longer egg-laying period leading to osteoporosis.

Mechanical Anisotropy of Individual Osteons in Bone Tissue at High Spatial Resolutions

2009 ◽  
Author(s):  
Davide Carnelli ◽  
Haimin Yao ◽  
Ming Dao ◽  
Pasquale Vena ◽  
Roberto Contro ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Cortical Bone ◽  
Mechanical Anisotropy ◽  
Haversian Canal ◽  
Maximum Penetration ◽  
Fundamental Units ◽  
Anisotropy Of Mechanical Properties ◽  
Penetration Depths ◽  
Secondary Osteons ◽  
Mineralized Collagen

Secondary osteons, the fundamental units of cortical bone, consist of cylindrical lamellar composites composed of mineralized collagen fibrils. Due to its lamellar structure, a multiscale knowledge of the mechanical properties of cortical bone is required to understand the biomechanical function of the tissue. In this light, nanoindentation tests were performed along the axial and transverse directions following a radial path from the Haversian canal to the osteonal edges. Different length scales are explored by means of indentations at different maximum penetration depths. Indentation moduli and hardness data were then interpreted in the context of the known microstructure. Results suggest that secondary osteons hierarchical structure is responsible for an observed length scale effect, homogenization phenomena and anisotropy of mechanical properties.

scholarly journals A Novel Green Extraction Technique for Extracting Flavonoids from Folium nelumbinis by Changing Osmosis Pressure

Materials ◽  
2020 ◽  
Vol 13 (18) ◽  
pp. 4192
Author(s):  
Hai-Yan Fang ◽  
Ying-Qin Wei ◽  
Meng-Li Zhang ◽  
Wei Liu
Keyword(s):  
Extraction Efficiency ◽  
Chemical Potential ◽  
Volume Fraction ◽  
Antioxidant Activities ◽  
Morphological Changes ◽  
Extraction Process ◽  
Extraction Technique ◽  
Salting Out ◽  
Digital Microscope ◽  
Main Factor

A new green and sustainable extraction technique, namely osmosis extraction (OE), was developed for efficient extracting flavonoids from Folium nelumbinis by changing the osmotic pressure. The antioxidant activities of the extracted flavonoids were also evaluated. Ethanol and ammonium sulfate were selected for the OE system because they are environmentally friendly. The maximum flavonoids concentration in the top phase was obtained with an ethanol volume fraction of 42.0% and the salt mass of 1.9 g. The kinetic behavior of the extraction process showed that OE had higher efficiencies especially coupled with ultrasonication due to the accompanying and serious morphological changes of Folium nelumbinis cells observed by digital microscope and nano-computed tomography (nano-CT). Results of morphological and anatomical features showed that the higher intracellular chemical potential made the cell expand and even led to bursting. The results also showed that the extraction efficiency of flavonoids with high antioxidant activities was higher than that of the traditional method. The interface effect enhanced the extraction during the salting-out extraction and osmosis was the main factor that improved the extraction efficiency.

scholarly journals Interface-resolved simulations of particle suspensions in Newtonian, shear thinning and shear thickening carrier fluids

2018 ◽  
Vol 852 ◽  
pp. 329-357 ◽  
Author(s):  
Dhiya Alghalibi ◽  
Iman Lashgari ◽  
Luca Brandt ◽  
Sarah Hormozi
Keyword(s):  
Volume Fraction ◽  
Solid Volume Fraction ◽  
Shear Thickening ◽  
Fluid Phase ◽  
Local Strain ◽  
Reynolds Numbers ◽  
Mean Value ◽  
Strain Rates ◽  
Local Shear ◽  
The Mean

We present a numerical study of non-colloidal spherical and rigid particles suspended in Newtonian, shear thinning and shear thickening fluids employing an immersed boundary method. We consider a linear Couette configuration to explore a wide range of solid volume fractions ($0.1\leqslant \unicode[STIX]{x1D6F7}\leqslant 0.4$) and particle Reynolds numbers ($0.1\leqslant Re_{p}\leqslant 10$). We report the distribution of solid and fluid phase velocity and solid volume fraction and show that close to the boundaries inertial effects result in a significant slip velocity between the solid and fluid phase. The local solid volume fraction profiles indicate particle layering close to the walls, which increases with the nominal $\unicode[STIX]{x1D6F7}$. This feature is associated with the confinement effects. We calculate the probability density function of local strain rates and compare the latter’s mean value with the values estimated from the homogenisation theory of Chateau et al. (J. Rheol., vol. 52, 2008, pp. 489–506), indicating a reasonable agreement in the Stokesian regime. Both the mean value and standard deviation of the local strain rates increase primarily with the solid volume fraction and secondarily with the $Re_{p}$. The wide spectrum of the local shear rate and its dependency on $\unicode[STIX]{x1D6F7}$ and $Re_{p}$ point to the deficiencies of the mean value of the local shear rates in estimating the rheology of these non-colloidal complex suspensions. Finally, we show that in the presence of inertia, the effective viscosity of these non-colloidal suspensions deviates from that of Stokesian suspensions. We discuss how inertia affects the microstructure and provide a scaling argument to give a closure for the suspension shear stress for both Newtonian and power-law suspending fluids. The stress closure is valid for moderate particle Reynolds numbers, $O(Re_{p})\sim 10$.

scholarly journals Histomorphometric variance of haversian canal in cortical bone of Malaysian ethnic groups

2018 ◽  
Vol 1019 ◽  
pp. 012009
Author(s):  
Hadi Abdullah ◽  
Muhammad Mahadi Abdul Jamil ◽  
Faridah Mohd Nor
Keyword(s):  
Cortical Bone ◽  
Ethnic Groups ◽  
Haversian Canal

scholarly journals Homogenization of cortical bone reveals that the organization and shape of pores marginally affect elasticity

2019 ◽  
Vol 16 (151) ◽  
pp. 20180911 ◽  
Author(s):  
Xiran Cai ◽  
Renald Brenner ◽  
Laura Peralta ◽  
Cécile Olivier ◽  
Pierre-Jean Gouttenoire ◽  
...  
Keyword(s):  
Cortical Bone ◽  
Volume Fraction ◽  
Transversely Isotropic ◽  
Pore Network ◽  
Anisotropic Elasticity ◽  
Homogenization Method ◽  
Femoral Diaphysis ◽  
Micro Computed Tomography ◽  
Architectural Features ◽  
Resonant Ultrasound

With ageing and various diseases, the vascular pore volume fraction (porosity) in cortical bone increases, and the morphology of the pore network is altered. Cortical bone elasticity is known to decrease with increasing porosity, but the effect of the microstructure is largely unknown, while it has been thoroughly studied for trabecular bone. Also, popular micromechanical models have disregarded several micro-architectural features, idealizing pores as cylinders aligned with the axis of the diaphysis. The aim of this paper is to quantify the relative effects on cortical bone anisotropic elasticity of porosity and other descriptors of the pore network micro-architecture associated with pore number, size and shape. The five stiffness constants of bone assumed to be a transversely isotropic material were measured with resonant ultrasound spectroscopy in 55 specimens from the femoral diaphysis of 29 donors. The pore network, imaged with synchrotron radiation X-ray micro-computed tomography, was used to derive the pore descriptors and to build a homogenization model using the fast Fourier transform (FFT) method. The model was calibrated using experimental elasticity. A detailed analysis of the computed effective elasticity revealed in particular that porosity explains most of the variations of the five stiffness constants and that the effects of other micro-architectural features are small compared to usual experimental errors. We also have evidence that modelling the pore network as an ensemble of cylinders yields biased elasticity values compared to predictions based on the real micro-architecture. The FFT homogenization method is shown to be particularly efficient to model cortical bone.

High Volume Fraction Aluminum /Alumina-Fused Silica Hybrid Particulate Metal Matrix Composite

2013 ◽  
Vol 701 ◽  
pp. 3-7
Author(s):  
Ghodratollah Roudini ◽  
Mehdi Asgharian ◽  
Morteza Khosravi
Keyword(s):  
Metal Matrix ◽  
Volume Fraction ◽  
High Volume ◽  
Optical Microscope ◽  
Fused Silica ◽  
High Volume Fraction ◽  
Matrix Composites ◽  
Sem Images ◽  
Composite Microstructure ◽  
Silica Hybrid

High volume fraction Aluminum/alumina-fused silica hybrid metal matrix composites containing alumina with 0, 10, 30 and 50 wt% fused silica were produced by melt squeezing casting method. Microstructure of hybrid composite was investigated by optical microscope and scanning electron microscopy (SEM). The SEM images showed uniform distribution of fused silica particles in composite microstructure. Also compressive strength of the composites changed (310-110 MPa) with amount of fused silica.

scholarly journals Orientation and size-dependent mechanical modulation within individual secondary osteons in cortical bone tissue

2013 ◽  
Vol 10 (81) ◽  
pp. 20120953 ◽  
Author(s):  
Davide Carnelli ◽  
Pasquale Vena ◽  
Ming Dao ◽  
Christine Ortiz ◽  
Roberto Contro
Keyword(s):  
Mechanical Properties ◽  
Cortical Bone ◽  
Mechanical Behaviour ◽  
Long Bone ◽  
Periodic Pattern ◽  
Haversian Canal ◽  
Length Scale ◽  
Lamellar Bone ◽  
Mechanical Modulation ◽  
Secondary Osteons

Anisotropy is one of the most peculiar aspects of cortical bone mechanics; however, its anisotropic mechanical behaviour should be treated only with strict relationship to the length scale of investigation. In this study, we focus on quantifying the orientation and size dependence of the spatial mechanical modulation in individual secondary osteons of bovine cortical bone using nanoindentation. Tests were performed on the same osteonal structure in the axial (along the long bone axis) and transverse (normal to the long bone axis) directions along arrays going radially out from the Haversian canal at four different maximum depths on three secondary osteons. Results clearly show a periodic pattern of stiffness with spatial distance across the osteon. The effect of length scale on lamellar bone anisotropy and the critical length at which homogenization of the mechanical properties occurs were determined. Further, a laminate-composite-based analytical model was applied to the stiffness trends obtained at the highest spatial resolution to evaluate the elastic constants for a sub-layer of mineralized collagen fibrils within an osteonal lamella on the basis of the spatial arrangement of the fibrils. The hierarchical arrangement of lamellar bone is found to be a major determinant for modulation of mechanical properties and anisotropic mechanical behaviour of the tissue.

scholarly journals Cortical Bone Modifications after Radiotherapy: Cortex Porosity and Osteonal Changes Evaluated Over Time

2021 ◽  
Vol 32 (1) ◽  
pp. 9-15
Author(s):  
Juliana Simeão Borges ◽  
Gustavo Davi Rabelo ◽  
Milena Suemi Irie ◽  
João Lucas Carvalho Paz ◽  
Rubens Spin-Neto ◽  
...  
Keyword(s):  
Cortical Bone ◽  
Cortical Thickness ◽  
Bone Microarchitecture ◽  
Total Porosity ◽  
Control Area ◽  
Bone Volume ◽  
Control Group ◽  
Significant Difference ◽  
Area And Perimeter ◽  
Haversian Canals

Abstract Aiming to evaluate cortical bone microarchitecture and osteonal morphology after irradiation, twelve male New Zealand rabbits were used. The animals were divided: control group (no radiation-NIr); and 3 irradiated groups, sacrificed after: 7 (Ir7d); 14 (Ir14d) and 21 (Ir21d) days. A single radiation dose of 30 Gy was used. Computed microtomography analyzed the cortical microarchitecture: cortical thickness (CtTh), bone volume (BV), total porosity (Ct.Po), intracortical porosity (CtPo-cl), channel/pore number (Po.N), fractal dimension (FD) and degree of anisotropy (Ct.DA). After scan, osteonal morphology was histologically assessed by means: area and perimeter of the osteons (O.Ar; O.p) and of the Haversian canals (C.Ar; C.p). Microtomographic analysis were performed by ANOVA, followed by Tukey and Dunnet tests. Osteon morphology analyses were performed by Kruskal-Wallis, and test Dunn’s. Cortical thickness was significant difference (p<0.010) between the NIr and irradiated groups, with thicker cortex at Ir7d (1.15±0.09). The intracortical porosity revealed significant difference (p<0.001) between irradiated groups and NIr, with lower value for Ir7d (0.29±0.09). Bone volume was lower in Ir14d compared to control. Area and perimeter of the osteons were statistically different (p<0.0001) between NIr and Ir7d. Haversian canals also revealed lower values (p<0.0001) in Ir7d (80.57±9.3; 31.63±6.5) compared to NIr and irradiated groups. Cortical microarchitecture was affected by radiation, and the effects appear to be time-dependent, mostly regarding the osteons morphology at the initial days. Cortex structure in Ir21d revealed similarities to control suggesting that microarchitecture resembles normal condition after a period.

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