Effects of Oral Supplementation With Plant Superoxide Dismutase Extract on Selected Redox Parameters and an Inflammatory Marker in a 2,000-m Rowing-Ergometer Test

2011 ◽  
Vol 21 (2) ◽  
pp. 124-134 ◽  
Author(s):  
Anna Skarpanska-Stejnborn ◽  
Lucja Pilaczynska-Szczesniak ◽  
Piotr Basta ◽  
Ewa Deskur-Smielecka ◽  
Donata Woitas-Slubowska ◽  
...  
Keyword(s):  
Superoxide Dismutase ◽  
Placebo Group ◽  
Inflammatory Marker ◽  
Thiobarbituric Acid ◽  
Creatine Kinase Activity ◽  
C Reactive Protein ◽  
Sod Activity ◽  
Blood Samples ◽  
Reactive Protein ◽  
Rowing Ergometer

The aim of this study was to investigate the effect of plant superoxide dismutase extract (GliSODin) supplementation on the balance of oxidants and antioxidants in the serum and erythrocytes of competitive rowers. The double-blinded study included 19 members of the Polish rowing team who were participating in a preparatory camp. Subjects were randomly assigned to the supplemented group (n = 10), who received 2 capsules (500 mg) of GliSODin extract once daily for 6 weeks, or the placebo group (n = 9). At the beginning and end of the study, subjects performed a 2,000-m maximum-effort test on a rowing ergometer. Blood samples were taken from the antecubital vein before each exercise test, 1 min after completing the test, and after a 24-hr restitution period. The following redox parameters were assessed in erythrocytes: superoxide dismutase (SOD) activity, glutathione peroxidase activity, and concentrations of thiobarbituric-acid-reactive substances. In addition, creatine kinase activity and total antioxidant capacity were measured in plasma samples, lactate levels were determined in capillary blood samples, and C-reactive protein and lactate dehydrogenase concentrations were measured in serum. After supplementation, SOD activity was significantly higher (p = .0037) in the supplemented group than the placebo group, and C-reactive protein was significantly (p = .00001) lower in athletes receiving GliSODin than those in the placebo group. In conclusion, supplementation with an extract rich in SOD activity promoted antioxidant status and protected against increased inflammation in the serum of professional rowers but had no effect on oxidative damage induced by exhaustive exercise.

The Influence of Supplementation with Rhodiola rosea L. Extract on Selected Redox Parameters in Professional Rowers

2009 ◽  
Vol 19 (2) ◽  
pp. 186-199 ◽  
Author(s):  
Anna Skarpanska-Stejnborn ◽  
Lucja Pilaczynska-Szczesniak ◽  
Piotr Basta ◽  
Ewa Deskur-Smielecka
Keyword(s):  
Superoxide Dismutase ◽  
Placebo Group ◽  
Antioxidant Capacity ◽  
Thiobarbituric Acid ◽  
Creatine Kinase Activity ◽  
Superoxide Dismutase Activity ◽  
Rhodiola Rosea ◽  
Blood Samples ◽  
Plasma Antioxidant Capacity ◽  
Lactate Levels

The aim of this study was to investigate the effect of Rhodiola rosea supplementation on the balance of oxidants and antioxidants in the serum and erythrocytes of competitive rowers. This double-blinded study included 22 members of the Polish Rowing Team who were participating in a preparatory camp. Participants were randomly assigned to the supplemented group (n = 11), who received 100 mg of R. rosea extract twice daily for 4 wk, or the placebo group (n = 11). At the beginning and end of the study, participants performed a 2,000-m maximum test on a rowing ergometer. Blood samples were taken from the antecubital vein before each exercise test, 1 min after completing the test, and after a 24-hr restitution period. The following redox parameters were assessed in erythrocytes: superoxide dismutase activity, glutathione peroxidase activity, and thiobarbituric-acid-reactive substances concentrations. In addition, creatine kinase activity and total antioxidant capacity were measured in plasma samples, lactate levels were determined in capillary blood samples, and uric acid concentrations were measured in serum. After supplementation, the total plasma antioxidant capacity was significantly higher (p = .0002) in the supplemented group than in the placebo group, and superoxide dismutase activity in erythrocytes directly after and 24 hr after the ergometry was significantly (p = .0461) lower in athletes receiving R. rosea extracts than in the placebo group. In conclusion, supplementation with R. rosea increased antioxidant levels in the plasma of professional rowers but had no effect on oxidative damage induced by exhaustive exercise.

Carbohydrate Influences Plasma Interleukin-6 but Not C-Reactive Protein or Creatine Kinase Following a 32-Km Mountain Trail Race

2006 ◽  
Vol 16 (1) ◽  
pp. 36-46 ◽  
Author(s):  
Mary P. Miles ◽  
Erin E. Walker ◽  
Stephen B. Conant ◽  
Shelly P. Hogan ◽  
Jessy R. Kidd
Keyword(s):  
Creatine Kinase ◽  
Interleukin 6 ◽  
Kinase Activity ◽  
Creatine Kinase Activity ◽  
C Reactive Protein ◽  
Blood Samples ◽  
Reactive Protein ◽  
Ad Libitum ◽  
Voluntary Intake ◽  
Exercise Induced

Attenuation of exercise-induced interleukin-6 (IL-6) responses by carbohydrate (CHO) has been demonstrated in studies comparing controlled doses (≥ 0.9 g · kg−1 · h−1) to placebo, but not in studies of voluntary intake. This study sought to determine if attenuation of the IL-6 response during a 32.2-km mountain trail race occurs for high compared to low ad libitum CHO intakes. IL-6, C-reactive protein (CRP), and creatine kinase activity (CK) were analyzed from blood samples collected 12 h pre-, 0, 4, and 24 h post-race. Subjects were grouped into low (n = 14, 0.4 ± 0.1 g · kg−1· h−1) and high (n = 18, 0.8 ± 0.2 g · kg−1 · h−1) CHO intake groups. IL-6 0 h post-race (P < 0.05) was higher in the low (40.2 ± 22.7 pg · mL−1) compared to the high CHO group (32.7 ± 22.1 pg · mL−1). CRP and CK both increased post-race, but no differences were observed between groups. Attenuation of exercise-induced IL-6 is apparent across a range of CHO intakes.

Evaluation of biomarkers of stress in chronic stress-exposed comorbid depression model Wistar rats

2019 ◽  
Vol 30 (5) ◽  
Author(s):  
Megha Gokul ◽  
Nayanatara Arun Kumar ◽  
Rekha Durgadas Kini ◽  
Vandana Blossom ◽  
Bhagyalakshmi Kodavanji ◽  
...  
Keyword(s):  
Chronic Stress ◽  
Inflammatory Marker ◽  
Human Life ◽  
Control Group ◽  
Chronic Unpredictable Stress ◽  
C Reactive Protein ◽  
Comorbid Depression ◽  
Blood Samples ◽  
Reactive Protein ◽  
Stressed Group

Abstract Background In recent years, increased stress in human life has a dual effect on brain and body physiology. Chronic stress takes a toll on physiology as well as on quality of life, ultimately leading to affective disorders. Rodent models are indispensable tools for studying the etiology and progress of depression. C-reactive protein has been proposed as a novel inflammatory marker. Methods Rats were divided into control and experimental stress groups (n = 6 each). The experimental group consisted of rats that were exposed to a set of chronic unpredictable stressors for 15 days. At the end of the 15th day, the animals were anesthetized, and blood samples were collected through cardiac puncture. Then the blood samples were analyzed for selected biochemical and oxidative stress parameters. Results Serum glutamic oxaloacetic transaminase (p < 0.0001), serum glutamic pyruvic transaminase (p < 0.001), serum malondialdehyde (p < 0.0001), total antioxidant level (p < 0.0001), and serum cortisol (p < 0.0001) were significantly increased in the stressed group when compared with the control group. C-reactive protein significantly (p < 0.0001) increased in the stressed group when compared with the control group. Conclusion Our results demonstrate that chronic unpredictable stress ameliorated depression-like behavior, which might have caused the dysregulation of the hypothalamic-pituitary-adrenal axis, causing the imbalance in the biochemical and oxidative parameters increasing the inflammatory markers. The inflammation-induced model of the chronic unpredictable stress model of comorbid depression might provide a variety of new targets for antidepressant therapies.

The Influence of Supplementation with Artichoke (Cynara scolymus L.) Extract on Selected Redox Parameters in Rowers

2008 ◽  
Vol 18 (3) ◽  
pp. 313-327 ◽  
Author(s):  
Anna Skarpañska-Stejnborn ◽  
Lucia Pilaczynska-Szczesniak ◽  
Piotr Basta ◽  
Ewa Deskur-Smielecka ◽  
Magorzata Horoszkiewicz-Hassan
Keyword(s):  
Placebo Group ◽  
Leaf Extract ◽  
Reductase Activity ◽  
Thiobarbituric Acid ◽  
Antioxidant Defense System ◽  
Creatine Kinase Activity ◽  
Antioxidant Potential ◽  
Blood Samples ◽  
Lactate Levels ◽  
Artichoke Leaf Extract

High-intensity physical exercise decreases intracellular antioxidant potential. An enhanced antioxidant defense system is desirable in people subjected to exhaustive exercise. The aim of this study was to investigate the influence of supplementation with artichoke-leaf extract on parameters describing balance between oxidants and antioxidants in competitive rowers. This double-blinded study was carried out in 22 members of the Polish rowing team who were randomly assigned to a supplemented group (n = 12), receiving 1 gelatin capsule containing 400 mg of artichoke-leaf extract 3 times a day for 5 wk, or a placebo group (n = 10). At the beginning and end of the study participants performed a 2,000-m maximal test on a rowing ergometer. Before each exercise test, 1 min after the test completion, and after a 24-hr restitution period blood samples were taken from antecubital vein. The following redox parameters were assessed in red blood cells: superoxide dismutase activity, glutathione peroxidase activity, glutathione reductase activity, reduced glutathione levels, and thiobarbituric-acid-reactive-substances concentrations. Creatine kinase activity and total antioxidant capacity (TAC) were measured in plasma samples, lactate levels were determined in capillary blood samples, and serum lipid profiles were assessed. During restitution, plasma TAC was significantly higher (p < .05) in the supplemented group than in the placebo group. Serum total cholesterol levels at the end of the study were significantly (p < .05) lower in the supplemented group than in the placebo group. In conclusion, consuming artichoke-leaf extract, a natural vegetable preparation of high antioxidant potential, resulted in higher plasma TAC than placebo but did not limit oxidative damage to erythrocytes in competitive rowers subjected to strenuous training.

C-reactive protein as a marker of progression of carotid atherosclerosis in subjects with type 2 diabetes mellitus

VASA ◽  
2017 ◽  
Vol 46 (3) ◽  
pp. 187-192 ◽  
Author(s):  
Aleš Pleskovič ◽  
Marija Šantl Letonja ◽  
Andreja Cokan Vujkovac ◽  
Jovana Nikolajević Starčević ◽  
Katarina Gazdikova ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Carotid Atherosclerosis ◽  
Inflammatory Marker ◽  
Progression Rate ◽  
C Reactive Protein ◽  
Reactive Protein ◽  
Hs Crp ◽  
Unstable Plaques

Abstract. Background: This prospective study was designed to evaluate the effect of inflammatory markers on the presence and progression of subclinical markers of carotid atherosclerosis in a 3.8-year follow-up period in patients with type 2 diabetes mellitus (T2DM). Patients and methods: A total of 595 subjects with T2DM were enrolled. Subclinical markers of carotid atherosclerosis (carotid intima media thickness (CIMT), plaque thickness, and plaques presence) were assessed with ultrasound at the time of recruitment and again after 3.8 years. Subjects with T2DM were divided into 2 groups according to the plasma high sensitive C-reactive protein (hs-CRP) levels (subjects with hs-CRP ≥ 2 mg/L and subjects with hs-CRP below 2 mg/L). Results: Subjects with T2DM and hs-CRP levels ≥ 2 mg/L had higher CIMT in comparison with subjects with T2DM and hs-CRP levels below 2 mg/L, and higher incidence of plaques/unstable plaques in comparison with subjects with T2DM and hs-CRP levels below 2 mg/L. Multivariate logistic regression analysis found the association between the HDL cholesterol level and presence of plaques, whereas the inflammatory marker hs-CRP was not associated with subclinical markers of progression of carotid atherosclerosis. Multiple linear regression analysis found the association between the hs-CRP levels and either CIMT progression rate or a change in the number of sites with plaques in a 3.8-year follow-up. Conclusions: We demonstrated an association between the inflammatory marker hs-CRP and either CIMT or incidence of plaques/unstable plaques at the time of recruitment in Caucasians with T2DM. Moreover, we found the association between hs-CRP levels and either CIMT progression rate or a change in the number of sites with plaques in a 3.8-year follow-up in subjects with T2DM.

Circulating phospholipase A2 and C-reactive protein after polytrauma—Effect of superoxide dismutase

1991 ◽  
Vol 23 (1) ◽  
pp. 2-4 ◽  
Author(s):  
R. Tikku ◽  
I. Marzi ◽  
J. Dike ◽  
O. Trentz ◽  
V. Bühren
Keyword(s):  
Superoxide Dismutase ◽  
Phospholipase A2 ◽  
C Reactive Protein ◽  
Reactive Protein

Comparison of the Neutrophil/Lymphocyte Ratio and C-Reactive Protein Levels in Patients With Amputation for Diabetic Foot Ulcers

2017 ◽  
Vol 16 (1) ◽  
pp. 23-28 ◽  
Author(s):  
Hasan Metineren ◽  
Turan Cihan Dülgeroğlu
Keyword(s):  
Diabetic Foot ◽  
Inflammatory Marker ◽  
Foot Ulcer ◽  
Diabetic Foot Ulcer ◽  
C Reactive Protein ◽  
Neutrophil Lymphocyte Ratio ◽  
Lymphocyte Ratio ◽  
Protein Levels ◽  
Reactive Protein ◽  
Before And After

This study compared the effectiveness of the neutrophil/lymphocyte ratio (NLR) versus C-reactive protein (CRP) for evaluating the prognosis and degree of inflammation in patients with amputation for a diabetic foot ulcer (DFU). This study enrolled 56 patients with amputations for DFU with gangrene and compared the CRP levels and NLR measured before and after surgery. Overall, 24 patients (42%) died within 2 weeks postoperatively. Mortality increased with a preoperative/postoperative CRP difference ≤1.5 ( P < .001) and age 73 years or older ( P < .001). The postoperative NLR was lower than the preoperative value but was not significant as a prognostic or inflammatory marker ( P = .945). An increasing serum CRP level is a significant predictor of mortality. CRP and old age are reliable prognostic factors in patients with DFU.

scholarly journals C-reactive protein as an inflammatory marker of acute infections outside intensive care settings: case report and evidence-based literature review

2011 ◽  
Vol 1 (4) ◽  
pp. 3-9
Author(s):  
Alcino Pires Gama ◽  
Itamar de Souza Santos ◽  
Rodrigo Diaz Olmosa ◽  
Lucia Mendes de Oliveira Pinto ◽  
Isabela Judith Martins Bensenor ◽  
...  
Keyword(s):  
Intensive Care ◽  
Case Report ◽  
Literature Review ◽  
Inflammatory Marker ◽  
Evidence Based ◽  
C Reactive Protein ◽  
Reactive Protein ◽  
Acute Infections

scholarly journals Gambaran kadar C-reactive protein (CRP) serum pada perokok aktif usia >40 tahun

2016 ◽  
Vol 4 (2) ◽  
Author(s):  
Hendrika N. C. Dewi ◽  
Michaela E. Paruntu ◽  
Murniati Tiho
Keyword(s):  
Inflammatory Marker ◽  
Systemic Disease ◽  
Toxic Chemical ◽  
C Reactive Protein ◽  
Cross Sectional ◽  
Reactive Protein ◽  
Positive Serum ◽  
Cross Sectional Design ◽  
Serum Crp ◽  
Descriptive Method

Abstract: Cigarette are the most cause of death around the world. Smoking cigarrete is harmful to the organs because it contains many toxic chemical that can stimulate inflammatory process. Smoking cigarrete is a risk factors for heart disease and chronic pulmonary disease (COPD). C-reactive protein (CRP) are a non spesific inflammatory marker that can elevated in both local and systemic disease. Beside biomarker, CRP also use as prognostic marker for inflammation. The purpose of this study was to identify the level of serum CRP of smokers aged >40 years old. Twenty eight smokers were eligible to this criteria and participated in this study. Cross-sectional design with a descriptive method was employed in this study. Sampels were analysed in laboratory for serum CRP levels. The results showed that 23 subjects (82%) had normal (negatif) serum CRP level and five subjects (18%) had positive serum CRP level. It can be concluded that serum CRP level of >40 years old smokers in Kolombo village, west Bitung two were mostly in normal level.Keywords: c-reactive protein (crp), active smoker, at age >40 years oldAbstrak: Rokok menjadi salah satu penyebab kematian terbesar di dunia. Merokok membahayakan hampir semua organ tubuh karena mengandung berbagai bahan kimia berbahaya yang dapat merangsang proses peradangan dan merupakan faktor utama penyakit jantung dan penyakit paru obstruktif kronik (PPOK). C-Reactive Protein (CRP) merupakan petanda inflamasi non-spesifik yang meningkat pada penyakit lokal maupun sistemik. Selain biomarker CRP juga dijadikan sebagai penanda prognostik untuk inflamasi. Tujuan dari penelitian ini untuk mengetahui kadar CRP serum pada perokok berusia >40 tahun. Dua puluh delapan perokok memenuhi syarat untuk kriteria ini dan berpartisipasi dalam penelitian ini. Penelitian ini menggunakan desain cross-sectional dengan metode deskriptif. Kadar CRP serum sampel dianalisis di laboratorium. Hasil penelitian menunjukkan bahwa 23 subyek (82%) memiliki kadar CRP serum normal (negatif) dan lima subjek (18%) memiliki kadar CRP serum positif. Dapat disimpulkan bahwa kadar CRP serum perokok berusia >40 tahun di desa Kolombo, Bitung barat dua kebanyakan dalam kadar normal.Kata kunci: c-reactive protein (crp), perokok aktif, usia >40 tahun.

scholarly journals Feasibility of a Novel Mobile C-Reactive Protein–Testing Device Using Gold-Linked Electrochemical Immunoassay: Clinical Performance Study (Preprint)

2020 ◽  
Author(s):  
Yuko Gondoh-Noda ◽  
Mitsuhiro Kometani ◽  
Akihiro Nomura ◽  
Daisuke Aono ◽  
Shigehiro Karashima ◽  
...  
Keyword(s):  
Performance Evaluation ◽  
Older Patients ◽  
Significant Positive Correlation ◽  
Electrochemical Immunoassay ◽  
Testing Device ◽  
C Reactive Protein ◽  
Blood Samples ◽  
Reactive Protein ◽  
Home Based ◽  
Home Based Care

BACKGROUND Home-based care is one of the most promising solutions to provide sufficient medical care for several older patients in Japan. However, because of insufficient diagnostic devices, it is sometimes difficult to detect early signs of the occurrence or worsening of diseases, such as infections under home-based care settings. C-reactive protein (CRP) is highly sensitive to diagnosing infections, and its elevation can help diagnose acute infection in older patients. Therefore, a CRP-measuring device that can be used in such a specific occasion is needed for home-based care. However, aspects such as its size, weight, and procedure are still challenging with respect to the practical use of mobile devices that quantitatively measure CRP levels easily and quickly under home-based care settings. OBJECTIVE We developed a new mobile, rapid CRP measurement device using a gold-linked electrochemical immunoassay (GLEIA) system. The aim of this study was to evaluate the feasibility of this mobile CRP-testing device. METHODS First, we assessed the performance of bare GLEIA-based electrode chips as the foundation of the device. After embedding the bare GLEIA-based electrode chips in a special plastic case and developing the mobile CRP-testing device, we further tested the device prototype using clinical blood samples. Finally, we evaluated the intra-assay variability for precision in the same condition and inter-assay variability for reproducibility in different conditions. RESULTS Blood samples for analysis were obtained by direct vein puncture from outpatients (N=85; females: 57/85; males: 28/85; age: 19-88 years) at Kanazawa University Hospital in Japan. For performance evaluation of bare GLEIA-based electrode chips, we used 85 clinical blood samples. There was a significant positive correlation between the electrode-predicted CRP levels and the reference CRP concentrations (R<sup>2</sup>=0.947; <i>P</i>&lt;.001). The assembled device was mobile (size 45×90×2.4 mm; weight 10 g) and disposable. The minimum volume of the sample needed for measuring CRP was 1.4 µL. The estimated preanalytical time was approximately 7 minutes and 40 seconds, and analysis time was approximately 1 minute and 10 seconds. Subsequently, for performance evaluation of the mobile CRP-testing device using GLEIA-based electrode chips, we used 26 clinical blood samples and found a significant positive correlation between the mobile device-predicted CRP levels and the reference CRP concentrations (R<sup>2</sup>=0.866, <i>P</i>&lt;.001). The intra-assay variabilities were 34.2%, 40.8%, and 24.5% for low, medium, and high CRP concentrations, respectively. The inter-assay variabilities were 46.5%, 38.3%, and 64.1% for low, medium, and high CRP concentrations, respectively. CONCLUSIONS Our findings suggest that this new mobile CRP-testing device might be suitable for use in home-based care settings.

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