The Influence of Supplementation with Artichoke (Cynara scolymus L.) Extract on Selected Redox Parameters in Rowers

2008 ◽  
Vol 18 (3) ◽  
pp. 313-327 ◽  
Author(s):  
Anna Skarpañska-Stejnborn ◽  
Lucia Pilaczynska-Szczesniak ◽  
Piotr Basta ◽  
Ewa Deskur-Smielecka ◽  
Magorzata Horoszkiewicz-Hassan
Keyword(s):  
Placebo Group ◽  
Leaf Extract ◽  
Reductase Activity ◽  
Thiobarbituric Acid ◽  
Antioxidant Defense System ◽  
Creatine Kinase Activity ◽  
Antioxidant Potential ◽  
Blood Samples ◽  
Lactate Levels ◽  
Artichoke Leaf Extract

High-intensity physical exercise decreases intracellular antioxidant potential. An enhanced antioxidant defense system is desirable in people subjected to exhaustive exercise. The aim of this study was to investigate the influence of supplementation with artichoke-leaf extract on parameters describing balance between oxidants and antioxidants in competitive rowers. This double-blinded study was carried out in 22 members of the Polish rowing team who were randomly assigned to a supplemented group (n = 12), receiving 1 gelatin capsule containing 400 mg of artichoke-leaf extract 3 times a day for 5 wk, or a placebo group (n = 10). At the beginning and end of the study participants performed a 2,000-m maximal test on a rowing ergometer. Before each exercise test, 1 min after the test completion, and after a 24-hr restitution period blood samples were taken from antecubital vein. The following redox parameters were assessed in red blood cells: superoxide dismutase activity, glutathione peroxidase activity, glutathione reductase activity, reduced glutathione levels, and thiobarbituric-acid-reactive-substances concentrations. Creatine kinase activity and total antioxidant capacity (TAC) were measured in plasma samples, lactate levels were determined in capillary blood samples, and serum lipid profiles were assessed. During restitution, plasma TAC was significantly higher (p < .05) in the supplemented group than in the placebo group. Serum total cholesterol levels at the end of the study were significantly (p < .05) lower in the supplemented group than in the placebo group. In conclusion, consuming artichoke-leaf extract, a natural vegetable preparation of high antioxidant potential, resulted in higher plasma TAC than placebo but did not limit oxidative damage to erythrocytes in competitive rowers subjected to strenuous training.

The Influence of Supplementation with Rhodiola rosea L. Extract on Selected Redox Parameters in Professional Rowers

2009 ◽  
Vol 19 (2) ◽  
pp. 186-199 ◽  
Author(s):  
Anna Skarpanska-Stejnborn ◽  
Lucja Pilaczynska-Szczesniak ◽  
Piotr Basta ◽  
Ewa Deskur-Smielecka
Keyword(s):  
Superoxide Dismutase ◽  
Placebo Group ◽  
Antioxidant Capacity ◽  
Thiobarbituric Acid ◽  
Creatine Kinase Activity ◽  
Superoxide Dismutase Activity ◽  
Rhodiola Rosea ◽  
Blood Samples ◽  
Plasma Antioxidant Capacity ◽  
Lactate Levels

The aim of this study was to investigate the effect of Rhodiola rosea supplementation on the balance of oxidants and antioxidants in the serum and erythrocytes of competitive rowers. This double-blinded study included 22 members of the Polish Rowing Team who were participating in a preparatory camp. Participants were randomly assigned to the supplemented group (n = 11), who received 100 mg of R. rosea extract twice daily for 4 wk, or the placebo group (n = 11). At the beginning and end of the study, participants performed a 2,000-m maximum test on a rowing ergometer. Blood samples were taken from the antecubital vein before each exercise test, 1 min after completing the test, and after a 24-hr restitution period. The following redox parameters were assessed in erythrocytes: superoxide dismutase activity, glutathione peroxidase activity, and thiobarbituric-acid-reactive substances concentrations. In addition, creatine kinase activity and total antioxidant capacity were measured in plasma samples, lactate levels were determined in capillary blood samples, and uric acid concentrations were measured in serum. After supplementation, the total plasma antioxidant capacity was significantly higher (p = .0002) in the supplemented group than in the placebo group, and superoxide dismutase activity in erythrocytes directly after and 24 hr after the ergometry was significantly (p = .0461) lower in athletes receiving R. rosea extracts than in the placebo group. In conclusion, supplementation with R. rosea increased antioxidant levels in the plasma of professional rowers but had no effect on oxidative damage induced by exhaustive exercise.

Effects of Oral Supplementation With Plant Superoxide Dismutase Extract on Selected Redox Parameters and an Inflammatory Marker in a 2,000-m Rowing-Ergometer Test

2011 ◽  
Vol 21 (2) ◽  
pp. 124-134 ◽  
Author(s):  
Anna Skarpanska-Stejnborn ◽  
Lucja Pilaczynska-Szczesniak ◽  
Piotr Basta ◽  
Ewa Deskur-Smielecka ◽  
Donata Woitas-Slubowska ◽  
...  
Keyword(s):  
Superoxide Dismutase ◽  
Placebo Group ◽  
Inflammatory Marker ◽  
Thiobarbituric Acid ◽  
Creatine Kinase Activity ◽  
C Reactive Protein ◽  
Sod Activity ◽  
Blood Samples ◽  
Reactive Protein ◽  
Rowing Ergometer

The aim of this study was to investigate the effect of plant superoxide dismutase extract (GliSODin) supplementation on the balance of oxidants and antioxidants in the serum and erythrocytes of competitive rowers. The double-blinded study included 19 members of the Polish rowing team who were participating in a preparatory camp. Subjects were randomly assigned to the supplemented group (n = 10), who received 2 capsules (500 mg) of GliSODin extract once daily for 6 weeks, or the placebo group (n = 9). At the beginning and end of the study, subjects performed a 2,000-m maximum-effort test on a rowing ergometer. Blood samples were taken from the antecubital vein before each exercise test, 1 min after completing the test, and after a 24-hr restitution period. The following redox parameters were assessed in erythrocytes: superoxide dismutase (SOD) activity, glutathione peroxidase activity, and concentrations of thiobarbituric-acid-reactive substances. In addition, creatine kinase activity and total antioxidant capacity were measured in plasma samples, lactate levels were determined in capillary blood samples, and C-reactive protein and lactate dehydrogenase concentrations were measured in serum. After supplementation, SOD activity was significantly higher (p = .0037) in the supplemented group than the placebo group, and C-reactive protein was significantly (p = .00001) lower in athletes receiving GliSODin than those in the placebo group. In conclusion, supplementation with an extract rich in SOD activity promoted antioxidant status and protected against increased inflammation in the serum of professional rowers but had no effect on oxidative damage induced by exhaustive exercise.

Influence of Aloe Arborescens Mill. Extract on Selected Parameters of Pro-oxidant-Antioxidant Equilibrium and Cytokine Synthesis in Rowers

2013 ◽  
Vol 23 (4) ◽  
pp. 388-398 ◽  
Author(s):  
Piotr Basta ◽  
Łucja Pilaczyńska-Szczȩśniak ◽  
Donata Woitas-Ślubowska ◽  
Anna Skarpańska-Stejnborn
Keyword(s):  
Placebo Group ◽  
Recovery Period ◽  
Thiobarbituric Acid ◽  
Creatine Kinase Activity ◽  
Double Blind Study ◽  
Double Blind ◽  
Maximum Test ◽  
Before And After ◽  
Total Antioxidant ◽  
Aloe Arborescens

This investigation examined the effect of supplementation with Biostimine, extract from aloe arborescens Mill. leaves, on the levels of pro-oxidant–antioxidant equilibrium markers and anti- and proinflammatory cytokines in rowers subjected to exhaustive exercise. This double-blind study included 18 members of the Polish Rowing Team. Subjects were randomly assigned to the supplemented group (n = 9), which received one ampoule of Biostimine once daily for 4 weeks, or to the placebo group (n = 9). Subjects performed a 2,000-meter-maximum test on a rowing ergometer at the beginning and end of the preparatory camp. Blood samples were obtained from the antecubital vein before each exercise test, 1 min after completing the test and after a 24-hr recovery period. Superoxide dismutase and glutathione peroxidase activity as well as the concentration of thiobarbituric acid reactive substances (TBARS) were assessed in erythrocytes. In addition, total antioxidant capacity (TAC) and creatine kinase activity were measured in plasma samples, and cytokine (IL-6, IL-10) concentrations were determined in the serum. Before and after Biostimine supplementation, exercise significantly increased the values of SOD, IL-6, IL-10, and TBARS in both groups. However, postexercise and recovery levels of TBARS were significantly lower in athletes receiving Biostimine than in controls. After supplementation, TAC was the only variable with the level being significantly higher in the supplemented group than in the placebo group. Consequently, we can conclude that Biostimine supplementation reduces the postexercise level of TBARS by increasing the antioxidant activity of plasma but has no effect on inflammatory markers.

Osmolal and Anion Gaps in Patients Admitted to an Emergency Medical Department

1994 ◽  
Vol 13 (2) ◽  
pp. 131-134 ◽  
Author(s):  
Lars Aabakken ◽  
Kjerstin S. Johansen ◽  
Else-Berit Rydningen ◽  
Jan E. Bredesen ◽  
Steinar Øvrebø ◽  
...  
Keyword(s):  
Standard Deviation ◽  
Ethylene Glycol ◽  
Reference Values ◽  
Medical Department ◽  
Blood Samples ◽  
Emergency Medical ◽  
Unselected Population ◽  
The Mean ◽  
Lactate Levels ◽  
Osmolal Gap

1 Osmolal and anion gaps are helpful in the diagnosis and evaluation of intoxications with methanol and ethylene glycol. Reported reference values for osmolal gap and anion gap are -1 (± 6) mosm kg-1 H2O and 16 (± 2) mmol I -1, respectively. However, we have repeatedly found unexplained increased gaps in patients admitted to our department, and the relevance of the established reference values has been questioned. 2 Osmolal and anion gaps were determined in an unselected population of patients consecutively admitted to an emergency medical department. In the case of unexplained gaps, the blood samples were analysed with respect to the presence of alcohols and organic acids. 3 We included all accessible patients admitted during 14 days. Appropriate blood samples were obtained in 177 patients (88 male, 89 female), with a mean age of 65 years (range 17-94). 4 The mean and (standard deviation) for osmolal and anion gaps in our material were 5.2 mosm kg-1 H2O (7.0) and 12.9 mmol/l (4.2). Neither methanol nor ethylene-glycol was detected in serum from any patients. Small amounts of ethanol were found in 5 patients, and high lactate levels explained in part the most extensively increased anion gaps. However, the calculated analytical standard deviation accounted entirely for the variation in our material, and we suggest that the present reference values be adjusted.

scholarly journals Tribulus terrestris protects rat myocardium against isoproterenol-induced ischemic injury: role of HSP 70 and cardiac endogenous antioxidants

2011 ◽  
Vol 1 (1) ◽  
pp. 9
Author(s):  
Ipseeta Ray Mohanty ◽  
Ujjwala Maheswari ◽  
Daniel Joseph ◽  
Yeshwant Deshmukh
Keyword(s):  
Myocardial Injury ◽  
Stress Protein ◽  
Myocardial Necrosis ◽  
Thiobarbituric Acid ◽  
Antioxidant Defense System ◽  
Control Group ◽  
Tribulus Terrestris ◽  
Marker Enzyme ◽  
Hsp 70 ◽  
Endogenous Antioxidants

The present study was undertaken to evaluate the cardioprotective activity of Tribulus terrestris (Tt), a medicinal herb following isoproterenol (ISP)-induced myocardial injury. The contribution of heat shock protein (HSP) 70, key anti-stress protein, endogenous antioxidants and oxidant -antioxidant balance in attenuating myocardial injury was further studied. Hydroalcoholic extract of Tt {1, 2.5, 5 &amp; 10 mg/kg} were orally fed once a daily to Wistar rats for 21 days. On the 20th and 21st day, both control (ISP control) and Tt fed rats were challenged with ISP (85 mg/ kg, s. c. two doses at 24h intervals) induced myocardial necrosis. Histopathological evaluation, cardiac marker enzyme: Creatinine phospho - kinase(CPK) and antioxidative parameters: Glutathione (GSH), Thiobarbituric acid reactive substances (TBARS), Catalase (CAT), Glutathione peroxidase (GSHPx) and Superoxide dismutase (SOD) levels were estimated. Tt (2.5 mg/kg) intake per se upregulated HSP 70; increased basal SOD, CAT activity (P&lt;0.05) and caused a marked fall in basal TBARS levels (P&lt;0.05) in comparison to sham. Following ISP challenge, significant oxidative stress with evidence of myocardial necrosis was observed in the ISP control group. ISPinduced changes in myocardial SOD, GSHPx and GSH were prevented by both the 2.5 and 10 mg/kg doses of Tt, though cellular injury was minimal with 2.5 mg/kg dose. The results emphasize that pre-treatment with Tt offered significant protection against ISP-induced myocardial necrosis through a unique property of enhancement of endogenous antioxidants, stabilization of cytoskeleton structure which in turn is attributed to HSP 70 expression along with fortified antioxidant defense system.

scholarly journals Antioxidant potential and cytotoxicity of Randia dumetorum Lam. leaf extract

2017 ◽  
Vol 9 (9) ◽  
pp. 138-145
Author(s):  
Abdullah-Al-Ragib ◽  
Tanvir Hossain Md. ◽  
Hossain Javed ◽  
Jakaria Md.
Keyword(s):  
Leaf Extract ◽  
Antioxidant Potential

scholarly journals Redox Status in Women with Rheumathoid Arthritis

2018 ◽  
Vol 0 (0) ◽  
Author(s):  
Aleksandra Vranic ◽  
Aleksandra Antovic ◽  
Nevena Draginic ◽  
Marijana Andjic ◽  
Marko Ravic ◽  
...  
Keyword(s):  
Rheumatoid Arthritis ◽  
Oxidative Stress ◽  
Hydrogen Peroxide ◽  
Lipid Peroxidation ◽  
Superoxide Dismutase ◽  
Cartilage Damage ◽  
Thiobarbituric Acid ◽  
Antioxidant Defense System ◽  
Redox Status ◽  
Female Population

Abstract The aim of this study was to assess oxidative status and to set baseline characteristics for female population with established rheumatoid arthritis. Total of 42 patients with rheumatoid arthritis and 48 age- and sex-matched controls were included in the study. Clinical examination was performed and assessed disease activity. Peripheral blood samples were used for all the assays. The markers of oxidative stress were assessed, including plasma levels of index of lipid peroxidation - thiobarbituric acid reactive substances, hydrogen peroxide, superoxide anion radical, nitrites and activity of superoxide dismutase, catalase and reduced glutathione levels as antioxidant parameters. In the patients group, levels of hydrogen peroxide and index of lipid peroxidation were higher than in controls. Patients with rheumatoid arthritis had decreased superoxide dismutase and catalase activity compared to healthy subjects. Interestingly, controls had higher levels of nitrites compared to patients. Patients showed a marked increase in reactive oxygen species formation and lipid peroxidation as well as decrease in the activity of antioxidant defense system leading to oxidative stress which may contribute to tissue and cartilage damage and hence to the chronicity of the disease.

Extraction, Isolation, Characterization and Antioxidant Potential of Pure Compounds from Adonsonia digitata Leaf extract

2019 ◽  
Author(s):  
Isaac John Umaru ◽  
Hauwa A. Umaru ◽  
Fasihuddin Badruddin Ahmed
Keyword(s):  
Leaf Extract ◽  
Bioactive Compound ◽  
Biological Properties ◽  
Antioxidant Potential ◽  
Chemical Components ◽  
Plant Parts ◽  
Chromatography Analysis ◽  
Isolation And Characterization ◽  
Pure Compounds ◽  
Anti Oxidant

Abstract Introduction Adonsonia digitata L. (Malvaceae) commonly known as Baobab is a medicinal and nutritional plant. The plant parts are used to treat various ailments such as diarrhoea, malaria and microbial infections. It is reported that it is an excellent anti-oxidant due to its vitamin C content. Baobab has numerous biological properties including antimicrobial, antiviral, anti-oxidant and anti-inflammatory activities amongst others. Objective: The study involves extraction, Isolation, Characterisation of phytochemicals and evaluation of antioxidant potential of the pure compounds. Methods: The dried leaf powder was subjected to rotary evaporator to obtain crude extract which was subjected to isolation using chromatography analysis and elucidation using NMR and FTIR. Antioxidant (IC 50 ) potential was determined using 2,2-diphenyl-1- picryl-hydrazyl-hydrate (DPPH). Result: Phytochemical investigation of the Baobab leaf extract through isolation and characterization of a bioactive compound was observed. The leaf extract yielded three compound characterised as D-Limonene, Thunbergol and (z)-2-Methyl-7-octadecene. Conclusion: The analysis of the chemical component reported identified compounds from Baobab leaf as; D-Limonene; ( 1 ) Thunbergol ( 2 ) and Cis-2-Methyl-7-octadecene ( 3 ) with significant antioxidant potential. The chemical components were identified for the first time.

scholarly journals Antioxidant Potential, Phytochemicals Composition, and Metal Contents of Datura alba

2019 ◽  
Vol 2019 ◽  
pp. 1-8 ◽  
Author(s):  
Waliullah Khan ◽  
Sidra Subhan ◽  
Dilawar Farhan Shams ◽  
Sahib Gul Afridi ◽  
Riaz Ullah ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Defense ◽  
Radical Scavenging ◽  
Pharmaceutical Formulations ◽  
Antioxidant Defense System ◽  
Antioxidant Potential ◽  
Defense System ◽  
Metal Contents ◽  
Stem Extract ◽  
Free Radical Scavenging Assay

This study investigated the phytochemical characteristics and antioxidant activity in leaves, roots, stem, flower, and seed parts of Datura alba (D. alba). The study also assessed the heavy metal (Cr, Mn, Zn, and Cu) accumulation in each part of the plant. Among the phytochemicals, alkaloids were found only in leaves while tannins, flavonoids, and phenols were present in all parts of the plant. For antioxidant activity, free radical scavenging assay for 2,2-diphenyl-1-picrylhydrazyl (DPPH) was performed using ascorbic acid as the standard. Higher activity was shown by stem extract in methanol and leaf extract in n-hexane, ethyl acetate, and chloroform. Furthermore, all the target heavy metals were detected in all plant sections with the highest concentration of Zn in leaves and Cu in stem, root, flower, and seed. Due to stronger antioxidant potential and phytochemical composition, D. alba could prove as valuable prospect in pharmaceutical formulations by taking part in the antioxidant defense system against generation of free radicals.

Artichoke leaf extract for treating hypercholesterolaemia

2016 ◽  
Author(s):  
Barbara Wider ◽  
Max H Pittler ◽  
Joanna Thompson-Coon ◽  
Edzard Ernst
Keyword(s):  
Leaf Extract ◽  
Artichoke Leaf Extract
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