The Effect of Progressive Resistance Training on Oxidative Stress and Antioxidant Enzyme Activity in Erythrocytes in Untrained Men

2013 ◽  
Vol 23 (3) ◽  
pp. 230-238 ◽  
Author(s):  
Kamal Azizbeigi ◽  
Mohammad Ali Azarbayjani ◽  
Maghsoud Peeri ◽  
Hamid Agha-alinejad ◽  
Stephen Stannard
Keyword(s):  
Oxidative Stress ◽  
Enzyme Activity ◽  
Resistance Training ◽  
Antioxidant Enzyme ◽  
Antioxidant Enzyme Activity ◽  
Progressive Resistance Training ◽  
Sod Activity ◽  
Free Radical Damage ◽  
Before And After ◽  
Progressive Resistance

This study was undertaken to investigate the effects of progressive resistance-training (PRT) on plasma oxidative stress and antioxidant enzyme activity in erythrocytes. Twenty male volunteers were randomly assigned to 2 groups: PRT and control. Blood samples were collected before and after 8 wk of PRT and analyzed for enzymatic activities of superoxide dismutase (SOD) and glutathione peroxidase (GPx) in erythrocytes, plasma total antioxidant capacity (TAC), and malondialdehyde concentration (MDA, an index of lipid per oxidation in plasma). Resistance training commenced with 8 exercises on nonconsecutive days for 8 wk at 50% of estimated 1-repetition maximum (E1RM) and reached 80% E1RM by Week 8. The results showed that PRT significantly increased erythrocyte SOD activity (1,323 ± 212.52 vs. 1,449.9 ± 173.8 U/g Hb, p = .014). Plasma concentration of MDA also decreased (5.39 ± 1.7 vs. 3.67.4 ± 0.7 nmol/ml, p = .030), although TAC (1.42 ± 0.21 vs. 1.61 ± 0.19 mmol/L, p = .1530) and GPx (39.87 ± 11.5 vs. 48.18 ± 14.48 U/g Hb, p = .883) activity did not undergo any considerable changes. Based on these data, the authors conclude that an 8-wk program of PRT strengthens the defensive system of erythrocytes against free-radical damage and therefore can be applied as a useful approach to alleviate oxidative stress.

scholarly journals Curcumin Acts as a Chemosensitizer for Leiomyosarcoma Cells In Vitro But Fails to Mediate Antioxidant Enzyme Activity in Cisplatin-Induced Experimental Nephrotoxicity in Rats

2019 ◽  
Vol 18 ◽  
pp. 153473541987281 ◽  
Author(s):  
Irida Dhima ◽  
Stelios Zerikiotis ◽  
Panagiotis Lekkas ◽  
Yannis V. Simos ◽  
Maria Gkiouli ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Enzyme Activity ◽  
Side Effects ◽  
Antioxidant Enzyme ◽  
Mitochondrial Function ◽  
Intraperitoneal Administration ◽  
Relative Weight ◽  
Antioxidant Enzyme Activity ◽  
Dose Finding ◽  
In Vivo Model

Background. Cisplatin (cis-diamminedichloroplatinum) is a widely used chemotherapeutic agent for the treatment of various cancers. Although it represents an effective regimen, its application is accompanied by side effects to normal tissues, especially to the kidneys. Cisplatin generates free radicals and impairs the function of antioxidant enzymes. Modulation of cisplatin-induced oxidative stress by specific antioxidant molecules represents an attractive approach to minimize side effects. Methods. We studied the ability of curcumin to sensitize leiomyosarcoma (LMS) cells to cisplatin. Assays for cell proliferation, mitochondrial function, induction of apoptosis, and cell cycle arrest were performed using various concentrations of cisplatin and a concentration of curcumin that caused a nonsignificant reduction in cell viability. Moreover, the effect of curcumin was examined against cisplatin-induced experimental nephrotoxicity. Renal injury was assessed by measuring serum creatinine, blood urea nitrogen (BUN), and the kidney’s relative weight. Oxidative stress was measured by means of enzymatic activities of superoxide dismutase and glutathione peroxidase in the rats’ blood and malondialdehyde levels in rats’ urine. Results. In our study, we found that curcumin sensitizes LMS cells to cisplatin by enhancing apoptosis and impairing mitochondrial function. In an in vivo model of cisplatin-induced experimental nephrotoxicity, intraperitoneal administration of curcumin failed to preserve blood’s antioxidant enzyme activity and decrease lipid peroxidation. Nevertheless, curcumin was able to protect nephrons’ histology from cisplatin’s toxic effect. Conclusion. Our results showed that curcumin can act as chemosensitizer, but its role as an adjunctive cisplatin-induced oxidative stress inhibitor requires further dose-finding studies to maximize the effectiveness of chemotherapy.

Effect of Oxidative Stress on Lymphocytes from Elderly Subjects

1998 ◽  
Vol 94 (4) ◽  
pp. 447-452 ◽  
Author(s):  
E. García-Arumí ◽  
A. L. Andreu ◽  
J. López-Hellín ◽  
S. Schwartz
Keyword(s):  
Oxidative Stress ◽  
Enzyme Activity ◽  
Oxidative Damage ◽  
Antioxidant Enzyme ◽  
Antioxidant Enzyme Activity ◽  
Elderly Subjects ◽  
Antioxidant Enzyme Activities ◽  
Antioxidant Defences ◽  
Protein Carbonyl Content ◽  
Healthy Elderly

1. Oxidative damage has been associated with ageing, but there is no agreement as to whether or not it is produced by a decrease in antioxidant defences with the ageing process. In purified lymphocytes from 47 healthy elderly (75.27 ± 0.91 years) and 47 healthy young (29.87 ± 0.53 years) volunteers, we studied the levels of antioxidant enzyme activity (superoxide dismutase, catalase and glutathione peroxidase), protein oxidative damage (as protein carbonyl content) and lysosomal proteolytic activity (cathepsins B, H and L), with and without exposure to oxidative stress produced by 25 μmol/l H2O2. 2. There were no differences in antioxidant enzyme activities in the stressed and non-stressed samples between the young and elderly subjects, indicating that there was no relationship between age and antioxidant enzyme activity even in oxidative stress. However, a dissimilar response to oxidative stress was observed in protein oxidative damage and cathepsin B and L activities, depending on the age of the donor. 3. With these results we conclude that oxidative stress produces greater protein oxidative damage and increased protein degradation in elderly subjects than in young ones; this effect cannot be attributed to dissimilar antioxidant enzyme responses to oxidative stress, since these did not differ between the two age groups.

scholarly journals Effect of Pulsed Ultraviolet Light on Natural Microbial Load and Antioxidant Properties of Fresh Blueberries

2018 ◽  
Vol 6 (6) ◽  
pp. 733
Author(s):  
Senem Guner ◽  
Zeynal Topalcengiz
Keyword(s):  
Enzyme Activity ◽  
Antioxidant Capacity ◽  
Antioxidant Enzyme ◽  
Antioxidant Properties ◽  
Antioxidant Enzyme Activity ◽  
Microbial Load ◽  
Plate Count ◽  
Light Illumination ◽  
Sod Activity ◽  
Aerobic Plate Count

Blueberries have been drawn more attention in recent years for their antioxidant capacities and proposed health benefits. Pulsed ultraviolet (PUV) light is known for its disinfection effects on the surface of fresh fruits. This study’s aim was to examine the effects of PUV light on naturally present microbial load, antioxidant capacity, and antioxidant enzyme activity of fresh blueberries. Fresh blueberry samples were treated for 30, 60, 90, and 120 s. Samples were stabilized at 13 cm away from the PUV lamp before treatment. Total Aerobic Plate Count (TAPC) and yeast and mold count reduction were observed the highest in samples treated for 90 s, 1.97 and 1.27 log CFU/g, respectively. Antioxidant capacity levels were significantly different for treated samples for 60 and 90 s in FRAP analysis, whereas no differences were observed in results of DPPH analysis. Superoxide dismutase (SOD) activity as antioxidant enzyme had no difference, however, glutathione peroxidase (GPx) increased by 50% with PUV treatment for 120 s. PUV light treatment of fresh blueberries for 90 s was determined as ideal exposure time among tested time intervals of this study. PUV light illumination can generally enhance antioxidant capacity and antioxidant enzyme activity of fresh blueberries and decrease the population of natural microorganisms of fresh blueberries and similar small fruits.

Comparison of melatonin versus vitamin C on oxidative stress and antioxidant enzyme activity in Alzheimer's disease induced by okadaic acid in neuroblastoma cells

2002 ◽  
Vol 451 (3) ◽  
pp. 237-243 ◽  
Author(s):  
Pedro Montilla-López ◽  
M.Carmen Muñoz-Águeda ◽  
Montserrat Feijóo López ◽  
Juan R Muñoz-Castañeda ◽  
Inmaculada Bujalance-Arenas ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Alzheimer’S Disease ◽  
Alzheimer's Disease ◽  
Enzyme Activity ◽  
Vitamin C ◽  
Antioxidant Enzyme ◽  
Okadaic Acid ◽  
Neuroblastoma Cells ◽  
Antioxidant Enzyme Activity

scholarly journals The Electrical Stimulation of the Bed Nucleus of the Stria Terminalis Causes Oxidative Stress in Skeletal Muscle of Rats

2018 ◽  
Vol 2018 ◽  
pp. 1-11 ◽  
Author(s):  
Mateusz Jakub Karnia ◽  
Dorota Myslinska ◽  
Katarzyna Patrycja Dzik ◽  
Damian Jozef Flis ◽  
Ziemowit Maciej Ciepielewski ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Skeletal Muscle ◽  
Enzyme Activity ◽  
Electrical Stimulation ◽  
Antioxidant Enzyme Activity ◽  
Stria Terminalis ◽  
Free Radical Damage ◽  
Bed Nucleus ◽  
Radical Damage ◽  
Stimulation Of

Recent studies indicate that activation of hypothalamus-pituitary-adrenocortical axis (HPA) plays the crucial role in stress response, while several lines of evidence mark the bed nucleus of the stria terminalis (BST) as a major mediator of the HPA axis responses to stress. The purpose of this study was to investigate the influence of the corticosterone flux induced by the electrical stimulation of BST on markers of free radical damage of lipids and proteins and antioxidant enzyme activity in skeletal muscle of rats. The male Wistar rats were used and assigned to one of three groups: sham-operated (SHM; n=6), two-week (ST2; n=6), and four-week stimulated (ST4; n=5) groups. Blood, soleus, and extensor digitorum longus muscles were collected. The chronic, 4-week electrical stimulation of the BST evokes increased plasma corticosterone concentration, which resulted in oxidative stress in skeletal muscles. We found higher level of lipid peroxidation markers, lower level of protein oxidation marker, and elevated antioxidant enzyme activity in both muscles. Our findings have also potential implication showing that reaction to the long-term “psychological stress” may lead to free radical damage of muscle.

Oxidative stress and antioxidant enzyme activity in South Indian male smokers with Chronic Obstructive Pulmonary Disease

Respirology ◽  
2013 ◽  
pp. n/a-n/a ◽  
Author(s):  
Arja Cholendra ◽  
Surapaneni Krishna Mohan ◽  
Raya Premanand ◽  
Adimoolam Chandrasekar ◽  
Balisetty Badhareenadhudu ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Chronic Obstructive Pulmonary Disease ◽  
Enzyme Activity ◽  
Antioxidant Enzyme ◽  
Pulmonary Disease ◽  
Antioxidant Enzyme Activity ◽  
Chronic Obstructive ◽  
Obstructive Pulmonary Disease ◽  
South Indian ◽  
Indian Male
Sign in / Sign up

Export Citation Format

Share Document