scholarly journals Fungal Bioweathering of Mimetite and a General Geomycological Model for Lead Apatite Mineral Biotransformations

2015 ◽  
Vol 81 (15) ◽  
pp. 4955-4964 ◽  
Author(s):  
Andrea Ceci ◽  
Martin Kierans ◽  
Stephen Hillier ◽  
Anna Maria Persiani ◽  
Geoffrey Michael Gadd

ABSTRACTFungi play important roles in biogeochemical processes such as organic matter decomposition, bioweathering of minerals and rocks, and metal transformations and therefore influence elemental cycles for essential and potentially toxic elements, e.g., P, S, Pb, and As. Arsenic is a potentially toxic metalloid for most organisms and naturally occurs in trace quantities in soil, rocks, water, air, and living organisms. Among more than 300 arsenic minerals occurring in nature, mimetite [Pb5(AsO4)3Cl] is the most stable lead arsenate and holds considerable promise in metal stabilization forin situandex situsequestration and remediation through precipitation, as do other insoluble lead apatites, such as pyromorphite [Pb5(PO4)3Cl] and vanadinite [Pb5(VO4)3Cl]. Despite the insolubility of mimetite, the organic acid-producing soil fungusAspergillus nigerwas able to solubilize mimetite with simultaneous precipitation of lead oxalate as a new mycogenic biomineral. Since fungal biotransformation of both pyromorphite and vanadinite has been previously documented, a new biogeochemical model for the biogenic transformation of lead apatites (mimetite, pyromorphite, and vanadinite) by fungi is hypothesized in this study by application of geochemical modeling together with experimental data. The models closely agreed with experimental data and provided accurate simulation of As and Pb complexation and biomineral formation dependent on, e.g., pH, cation-anion composition, and concentration. A general pattern for fungal biotransformation of lead apatite minerals is proposed, proving new understanding of ecological implications of the biogeochemical cycling of component elements as well as industrial applications in metal stabilization, bioremediation, and biorecovery.

Sensor Review ◽  
2015 ◽  
Vol 35 (2) ◽  
pp. 141-145 ◽  
Author(s):  
Richard Bloss

Purpose – The purpose of this paper is to review the recent advancements in the development of wearable sensors which can continuously monitor critical medical, assess athletic activity, watch babies and serve industrial applications. Design/methodology/approach – The paper presents an in-depth review of a number of developments in wearable sensing and monitoring technologies for medical, athletic and industrial applications. Researchers and companies around the world were contacted to discuss their direction and progress in this field of medical condition and industrial monitoring, as well as discussions with medical personnel on the perceived benefits of such technology. Findings – Dramatic progress is being made in continuous monitoring of many important body functions that indicate critical medical conditions that can be life-threatening, contribute to blindness or access activity. In the industrial arena, wearable devices bring remote monitoring to a new level. Practical implications – Doctors will be able to replace one-off tests with continuous monitoring that provides a much better continuous real-time “view” into the patient’s conditions. Wearable monitors will help provide much better medical care in the future. Industrial managers and others will be able to monitor and supervise remotely. Originality/value – An expert insight into advancements in medical condition monitoring that replaces the one-time “finger prick” type testing only performed in the doctor’s office. It is also a look at how wearable monitoring is greatly improved and serving athletics, the industry and parents.


2014 ◽  
Vol 25 (4) ◽  
pp. 568-598 ◽  
Author(s):  
Marco Macchi ◽  
Adolfo Crespo Márquez ◽  
Maria Holgado ◽  
Luca Fumagalli ◽  
Luis Barberá Martínez

Purpose – The purpose of this paper is to propose a methodology for the engineering of E-maintenance platforms that is based on a value-driven approach. Design/methodology/approach – The methodology assumes that a value-driven engineering approach would help foster technological innovation for maintenance management. Indeed, value-driven engineering could be easily adopted at the business level, with subsequent positive effects on the industrial applications of new information and communication technologies solutions. Findings – The methodology combines a value-driven approach with the engineering in the maintenance scope. The methodology is tested in a manufacturing case to prove its potential to support the engineering of E-maintenance solutions. In particular, the case study concerns the investment in E-maintenance solutions developed in the framework of a Supervisory Control and Data Acquisition system originally implemented for production purposes. Originality/value – Based on literature research, the paper presents a methodology that is implemented considering three different approaches (business theories, value-driven engineering and maintenance management). The combination of these approaches is novel and overcomes the traditional view of maintenance as an issue evaluated from a cost-benefit perspective.


2021 ◽  
Vol ahead-of-print (ahead-of-print) ◽  
Author(s):  
J. Norberto Pires ◽  
Amin S. Azar ◽  
Filipe Nogueira ◽  
Carlos Ye Zhu ◽  
Ricardo Branco ◽  
...  

Purpose Additive manufacturing (AM) is a rapidly evolving manufacturing process, which refers to a set of technologies that add materials layer-by-layer to create functional components. AM technologies have received an enormous attention from both academia and industry, and they are being successfully used in various applications, such as rapid prototyping, tooling, direct manufacturing and repair, among others. AM does not necessarily imply building parts, as it also refers to innovation in materials, system and part designs, novel combination of properties and interplay between systems and materials. The most exciting features of AM are related to the development of radically new systems and materials that can be used in advanced products with the aim of reducing costs, manufacturing difficulties, weight, waste and energy consumption. It is essential to develop an advanced production system that assists the user through the process, from the computer-aided design model to functional components. The challenges faced in the research and development and operational phase of producing those parts include requiring the capacity to simulate and observe the building process and, more importantly, being able to introduce the production changes in a real-time fashion. This paper aims to review the role of robotics in various AM technologies to underline its importance, followed by an introduction of a novel and intelligent system for directed energy deposition (DED) technology. Design/methodology/approach AM presents intrinsic advantages when compared to the conventional processes. Nevertheless, its industrial integration remains as a challenge due to equipment and process complexities. DED technologies are among the most sophisticated concepts that have the potential of transforming the current material processing practices. Findings The objective of this paper is identifying the fundamental features of an intelligent DED platform, capable of handling the science and operational aspects of the advanced AM applications. Consequently, we introduce and discuss a novel robotic AM system, designed for processing metals and alloys such as aluminium alloys, high-strength steels, stainless steels, titanium alloys, magnesium alloys, nickel-based superalloys and other metallic alloys for various applications. A few demonstrators are presented and briefly discussed, to present the usefulness of the introduced system and underlying concept. The main design objective of the presented intelligent robotic AM system is to implement a design-and-produce strategy. This means that the system should allow the user to focus on the knowledge-based tasks, e.g. the tasks of designing the part, material selection, simulating the deposition process and anticipating the metallurgical properties of the final part, as the rest would be handled automatically. Research limitations/implications This paper reviews a few AM technologies, where robotics is a central part of the process, such as vat photopolymerization, material jetting, binder jetting, material extrusion, powder bed fusion, DED and sheet lamination. This paper aims to influence the development of robot-based AM systems for industrial applications such as part production, automotive, medical, aerospace and defence sectors. Originality/value The presented intelligent system is an original development that is designed and built by the co-authors J. Norberto Pires, Amin S. Azar and Trayana Tankova.


2021 ◽  
Vol ahead-of-print (ahead-of-print) ◽  
Author(s):  
Jiawei Lian ◽  
Junhong He ◽  
Yun Niu ◽  
Tianze Wang

Purpose The current popular image processing technologies based on convolutional neural network have the characteristics of large computation, high storage cost and low accuracy for tiny defect detection, which is contrary to the high real-time and accuracy, limited computing resources and storage required by industrial applications. Therefore, an improved YOLOv4 named as YOLOv4-Defect is proposed aim to solve the above problems. Design/methodology/approach On the one hand, this study performs multi-dimensional compression processing on the feature extraction network of YOLOv4 to simplify the model and improve the feature extraction ability of the model through knowledge distillation. On the other hand, a prediction scale with more detailed receptive field is added to optimize the model structure, which can improve the detection performance for tiny defects. Findings The effectiveness of the method is verified by public data sets NEU-CLS and DAGM 2007, and the steel ingot data set collected in the actual industrial field. The experimental results demonstrated that the proposed YOLOv4-Defect method can greatly improve the recognition efficiency and accuracy and reduce the size and computation consumption of the model. Originality/value This paper proposed an improved YOLOv4 named as YOLOv4-Defect for the detection of surface defect, which is conducive to application in various industrial scenarios with limited storage and computing resources, and meets the requirements of high real-time and precision.


2021 ◽  
Vol ahead-of-print (ahead-of-print) ◽  
Author(s):  
Sakthi Sadhasivam RM ◽  
Ramanathan K. ◽  
Bhuvaneswari B.V. ◽  
Raja R.

Purpose The most promising replacements for the industrial applications are particle reinforced metal matrix composites because of their good and combined mechanical properties. Currently, the need of matrix materials for industrial applications is widely satisfied by aluminium alloys. The purpose of this paper is to evaluate the tribological behaviour of the zinc oxide (ZnO) particles reinforced AA6061 composites prepared by stir casting route. Design/methodology/approach In this study, AA6061 aluminium alloy matrix reinforced with varying weight percentages (3%, 4.5% and 6%) of ZnO particles, including monolithic AA6061 alloy samples, is cast by the most economical fabrication method, called stir casting. The prepared sample was subjected to X-ray photoelectron spectroscopy (XPS) analysis, experimental density measurement by Archimedian principle and theoretical density by rule of mixture and hardness test to investigate mechanical property. The dry sliding wear behaviour of the composites was investigated using pin-on-disc tribometer with various applied loads of 15 and 20 N, with constant sliding velocity and distance. The wear rate, coefficient of friction (COF) and worn surfaces of the composite specimens and their effects were also investigated in this work. Findings XPS results confirm the homogeneous distribution of ZnO microparticles in the Al matrix. The Vickers hardness result reveals that higher ZnO reinforced (6%) sample have 34.4% higher values of HV than the monolithic aluminium sample. The sliding wear tests similarly show that increasing the weight percentage of ZnO particles leads to a reduced wear rate and COF of 30.01% and 26.32% lower than unreinforced alloy for 15 N and 36.35% and 25% for 20 N applied load. From the worn surface morphological studies, it was evidently noticed that ZnO particles dispersed throughout the matrix and it had strong bonding between the reinforcement and the matrix, which significantly reduced the plastic deformation of the surfaces. Originality/value The uniqueness of this work is to use the reinforcement of ZnO particles with AA6061 matrix and preparing by stir casting route and to study and analyse the physical, hardness and tribological behaviour of the composite materials.


Circuit World ◽  
2021 ◽  
Vol ahead-of-print (ahead-of-print) ◽  
Author(s):  
Rohollah Abdollahi

Purpose The purpose of this paper is to provide a T autotransformer based 12-pulse rectifier with passive harmonic reduction in more electric aircraft applications. The T autotransformer uses only two main windings which result in volume, space, size, weight and cost savings. Also, the proposed unconventional inter-phase transformer (UIPT) with a lower kVA rating (about 2.6% of the load power) compared to the conventional inter-phase transformer results in a more harmonic reduction. Design/methodology/approach To increase rating and reduce the cost and complexity of a multi-pulse rectifier, it is well known that the pulse number must be increased. In some practical cases, a 12-pulse rectifier (12PR) is suggested as a good solution considering its simple structure and low weight. But the 12PR cannot technically meet the standards of harmonic distortion requirements for some industrial applications, and therefore, they must be used with output filters. In this paper, a 12PR is suggested, which consists of a T autotransformer 12PR and a passive harmonic reduction (PHR) based on the UIPT at direct current (DC) link. Findings To show the advantage of this new combination over other solutions, simulation results are used, and then, a prototype is implemented to evaluate and verify the simulation results. The simulation and experimental test results show that the input current total harmonic distortion (THD) of the suggested 12PR with a PHR based on UIPT is less than 5%, which meets the IEEE 519 requirements. Also, it is shown that in comparison with other solutions, it is cost effective, and at the same time, its power factor is near unity, and its rating is 29.92% of the load rating. Therefore, it is obvious that the proposed rectifier is a practical solution for more electric aircrafts. Originality/value The contributions of this paper are summarized as follows. The suggested design uses a retrofit T autotransformer, which meets all technical constraints, and in comparison, with other options, has less rating, weight, volume and cost. In the suggested rectifier, a PHR based on UIPT at its dc link of 12PR is used, which has good technical capabilities and lower ratings. In the PHR based on UIPT, an IPT is used, which has an additional secondary winding and four diodes. This solution leads to a reduction in input current THD and conduction losses of diodes. In full load conditions, the input line current THD and power factor are 4% and 0.99, respectively. The THD is less than 5%, which satisfies IEEE-519 and DO-160G requirements.


2021 ◽  
Vol ahead-of-print (ahead-of-print) ◽  
Author(s):  
Ansheng Zhang ◽  
Mingyu Zhang ◽  
Jing Wang ◽  
Jianjun Zhang ◽  
Zhaohua Shang ◽  
...  

Purpose The purpose of this paper is to study the influence of surface precision on the lubrication state of the roller chain under adequate and rare oil supply conditions, respectively. Design/methodology/approach The straightness error and roughness error of the pin generatrices were measured and the influence of surface precision on the lubrication behavior under steady state and reciprocating motion was studied through optical interference experiments. Findings The lubrication state is strongly influenced by the surface precision of the roller surface both under adequate oil supply and rare oil supply conditions. Originality/value In industrial applications, the machining errors of parts cannot be completely eliminated. Studying the influence of the surface precision on the lubrication behavior of pin–bush pairs can provide the experimental basis for the optimal design of the bush roller chains.


2018 ◽  
Vol 90 (7) ◽  
pp. 1136-1144 ◽  
Author(s):  
Dimitris Gkiolas ◽  
Demetri Yiasemides ◽  
Demetri Mathioulakis

Purpose The complex flow behavior over an oscillating aerodynamic body, e.g. a helicopter rotor blade, a rotating wind turbine blade or the wing of a maneuvering airplane involves combinations of pitching and plunging motions. As the parameters of the problem (Re, St and phase difference between these two motions) vary, a quasi-steady analysis fails to provide realistic results for the aerodynamic response of the moving body, whereas this study aims to provide reliable experimental data. Design/methodology/approach In the present study, a pitching and plunging mechanism was designed and built in a subsonic closed-circuit wind tunnel as well as a rectangular aluminum wing of a 2:1 aspect-ratio with a NACA64-418 airfoil, used in wind turbine blades. To measure the pressure distribution along the wing chord, a number of fast responding transducers were embedded into the mid span wing surface. Simultaneous pressure measurements were conducted along the wing chord for the Reynolds number of 0.85 × 106 for both steady and unsteady cases (pitching and plunging). A flow visualization technique was used to detect the flow separation line under steady conditions. Findings Elevated pressure fluctuations coincide with the flow separation line having been detected through surface flow visualization and flattened pressure distributions appear downstream of the flow separation line. Closed hysteresis loops of the lift coefficient versus angle of attack were measured for combined pitching and plunging motions. Practical implications The experimental data can be used for improvement of unsteady fluid mechanics problem solvers. Originality/value In the present study, a new installation was built allowing the aerodynamic study of oscillating wings performing pitching and plunging motions with prescribed frequencies and phase lags between the two motions. The experimental data can be used for improvement of computational fluid dynamics codes in case that the examined aerodynamic body is oscillating.


2020 ◽  
Vol 86 (17) ◽  
Author(s):  
Miha Bahun ◽  
Marko Šnajder ◽  
Dušan Turk ◽  
Nataša Poklar Ulrih

ABSTRACT Pernisine is a subtilisin-like protease that was originally identified in the hyperthermophilic archaeon Aeropyrum pernix, which lives in extreme marine environments. Pernisine shows exceptional stability and activity due to the high-temperature conditions experienced by A. pernix. Pernisine is of interest for industrial purposes, as it is one of the few proteases that has demonstrated prion-degrading activity. Like other extracellular subtilisins, pernisine is synthesized in its inactive pro-form (pro-pernisine), which needs to undergo maturation to become proteolytically active. The maturation processes of mesophilic subtilisins have been investigated in detail; however, less is known about the maturation of their thermophilic homologs, such as pernisine. Here, we show that the structure of pro-pernisine is disordered in the absence of Ca2+ ions. In contrast to the mesophilic subtilisins, pro-pernisine requires Ca2+ ions to adopt the conformation suitable for its subsequent maturation. In addition to several Ca2+-binding sites that have been conserved from the thermostable Tk-subtilisin, pernisine has an additional insertion sequence with a Ca2+-binding motif. We demonstrate the importance of this insertion for efficient folding and stabilization of pernisine during its maturation. Moreover, analysis of the pernisine propeptide explains the high-temperature requirement for pro-pernisine maturation. Of note, the propeptide inhibits the pernisine catalytic domain more potently at high temperatures. After dissociation, the propeptide is destabilized at high temperatures only, which leads to its degradation and finally to pernisine activation. Our data provide new insights into and understanding of the thermostable subtilisin autoactivation mechanism. IMPORTANCE Enzymes from thermophilic organisms are of particular importance for use in industrial applications, due to their exceptional stability and activity. Pernisine, from the hyperthermophilic archaeon Aeropyrum pernix, is a proteolytic enzyme that can degrade infective prion proteins and thus has a potential use for disinfection of prion-contaminated surfaces. Like other subtilisin-like proteases, pernisine needs to mature through an autocatalytic process to become an active protease. In the present study, we address the maturation of pernisine and show that the process is regulated specifically at high temperatures by the propeptide. Furthermore, we demonstrate the importance of a unique Ca2+-binding insertion for stabilization of mature pernisine. Our results provide a novel understanding of thermostable subtilisin autoactivation, which might advance the development of these enzymes for commercial use.


2012 ◽  
Vol 79 (6) ◽  
pp. 1777-1786 ◽  
Author(s):  
M. Popova ◽  
D. P. Morgavi ◽  
C. Martin

ABSTRACTThe amount and nature of dietary starch are known to influence the extent and site of feed digestion in ruminants. However, how starch degradability may affect methanogenesis and methanogens along the ruminant's digestive tract is poorly understood. This study examined the diversity and metabolic activity of methanogens in the rumen and cecum of lambs receiving wheat or corn high-grain-content diets. Methane productionin vivoandex situwas also monitored.In vivodaily methane emissions (CH4g/day) were 36% (P< 0.05) lower in corn-fed lambs than in wheat-fed lambs.Ex situmethane production (μmol/h) was 4-fold higher for ruminal contents than for cecal contents (P< 0.01), while methanogens were 10-fold higher in the rumen than in the cecum (mcrAcopy numbers;P< 0.01). Clone library analysis indicated thatMethanobrevibacterwas the dominant genus in both sites. Diet induced changes at the species level, as theMethanobrevibacter millerae-M. gottschalkii-M. smithiiclade represented 78% of the sequences from the rumen of wheat-fed lambs and just about 52% of the sequences from the rumen of the corn-fed lambs. Diet did not affectmcrAexpression in the rumen. In the cecum, however, expression was 4-fold and 2-fold lower than in the rumen for wheat- and corn-fed lambs, respectively. Though we had no direct evidence for compensation of reduced rumen methane production with higher cecum methanogenesis, the ecology of methanogens in the cecum should be better considered.


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