scholarly journals Biological Control of Hog Waste Odor through Stimulated Microbial Fe(III) Reduction

2005 ◽  
Vol 71 (8) ◽  
pp. 4728-4735 ◽  
Author(s):  
John D. Coates ◽  
Kimberly A. Cole ◽  
Urania Michaelidou ◽  
Jennifer Patrick ◽  
Michael J. McInerney ◽  
...  
Keyword(s):  
Volatile Fatty Acids ◽  
Swine Manure ◽  
Cost Effective ◽  
Complete Removal ◽  
The United States ◽  
Geobacter Sulfurreducens ◽  
Swine Waste ◽  
Geobacter Metallireducens ◽  
Pure Cultures ◽  
Production Facilities

ABSTRACT Odor control and disposal of swine waste have inhibited expansion of swine production facilities throughout the United States. Swine waste odor is associated primarily with high concentrations of volatile fatty acids (VFAs). Here, we demonstrate that stimulated Fe(III) reduction in hog manure can rapidly remove the malodorous compounds and enhance methane production by 200%. As part of these studies, we enumerated the indigenous Fe(III)-reducing population in swine waste and identified members of the family Geobacteraceae as the dominant species. These organisms were present at concentrations as high as 2 × 105 cells g−1. Several pure cultures of Fe(III) reducers, including Geobacter metallireducens, Geobacter humireducens, Geobacter sulfurreducens, Geobacter grbiciae, Geothrix fermentans, and Geovibrio ferrireducens, readily degraded some or all of the malodorous VFAs found in swine manure. In contrast, Shewanella algae did not degrade any of these compounds. We isolated an Fe(III) reducer, Geobacter strain NU, from materials collected from primary swine waste lagoons. This organism degraded all of the malodorous VFAs tested and readily grew in swine waste amended with Fe(III). When raw waste amended with Fe(III) was inoculated with strain NU, the VFA content rapidly decreased, corresponding with an almost complete removal of the odor. In contrast, the raw waste without Fe(III) or strain NU showed a marked increase in VFA content and a rapid pH drop. This study showed that Fe(III) supplementation combined with appropriate bioaugmentation provides a simple, cost-effective approach to deodorize and treat swine waste, removing a significant impediment to the expansion of pork production facilities.

Plant oils thymol and eugenol affect cattle and swine waste emissions differently

2004 ◽  
Vol 50 (4) ◽  
pp. 207-213 ◽  
Author(s):  
V.H. Varel ◽  
D.N. Miller ◽  
A.D. Lindsay
Keyword(s):  
Fatty Acids ◽  
Volatile Fatty Acids ◽  
Swine Manure ◽  
Environmental Problems ◽  
Coliform Bacteria ◽  
Distinct Advantage ◽  
Plant Oils ◽  
Swine Waste ◽  
Nutrient Emissions ◽  
Working Volume

Wastes generated from the production of cattle and swine in confined facilities create the potential for surface and groundwater pollution, emission of greenhouse gases, transmission of pathogens to food and water sources, and odor. It is our hypothesis that something which inhibits microbial fermentation in livestock wastes will be beneficial to solving some of the environmental problems. Our work has concentrated on the use of antimicrobial plant oils, thymol, thyme oil, carvacrol, eugenol and clove oil. Anaerobic one-litre flasks with a working volume of 0.5 L cattle or swine manure were used to evaluate the effect of thymol and eugenol on production of fermentation gas, short-chain volatile fatty acids, lactate, and bacterial populations. Either oil at 0.2% in both wastes essentially stopped all production of gas and volatile fatty acids, and eliminated all fecal coliform bacteria. In cattle but not swine waste, thymol prevented the accumulation of lactate. However, eugenol stimulated lactate formation in cattle and swine wastes. Thus, eugenol may offer a distinct advantage over thymol, because lactate accumulation in the wastes causes the pH to drop more rapidly, further inhibiting microbial activity and nutrient emissions. We conclude that plant oils may offer solutions to controlling various environmental problems associated with livestock wastes, assuming that they are cost-effective.

Detection of enteroviruses in marine waters by direct RT-PCR and cell culture

1995 ◽  
Vol 31 (5-6) ◽  
pp. 323-328 ◽  
Author(s):  
K. A. Reynolds ◽  
C. P. Gerba ◽  
I. L. Pepper
Keyword(s):  
Cell Culture ◽  
Polymerase Chain Reaction Amplification ◽  
Cost Effective ◽  
The United States ◽  
Water Runoff ◽  
Rt Pcr ◽  
Marine Waters ◽  
Storm Water Runoff ◽  
Routine Monitoring ◽  
Reaction Amplification

Sewage outfalls and storm water runoff introduces pathogenic human enteric viruses into marine coastal waters, which may pose a potential public health risk. Although members of the enterovirus group have been suggested as possible indicators of sewage pollution in marine waters, the lack of rapid, sensitive and cost effective methods have prevented routine monitoring in the United States. This study compared traditional cell culture and direct RT-PCR (reverse transcriptase-polymerase chain reaction) amplification for detection of an enterovirus. Poliovirus could be recovered from 100 L of artificial seawater with an average efficiency of 77%, using adsorption and elution from electronegative filters. Viruses were eluted from the filters with 1.5% beef extract for viruses (BEV) adjusted to pH 9.5 and reconcentrated by organic flocculation to a volume of 30 mL. Substances which interfered with detection by RT-PCR were removed by treatment of the concentrates with sephadex and chelex resins. Direct RT-PCR could detect 2.5 and 0.025 PFU (plaque forming units) for single (25 cycles) and double PCR (2 × 25 cycles) in 10 μL of pure culture poliovirus samples, respectively. These methods are currently being applied to assess the occurrence of enteroviruses at marine bathing beaches influenced by sewage discharges.

A Novel Approach for the Treatment of Sialolithiasis that Preserves Salivary Duct Anatomy

2021 ◽  
pp. 000348942110189
Author(s):  
Gani Atilla Şengör ◽  
Ahmet Mert Bilgili
Keyword(s):  
Salivary Gland ◽  
Success Rate ◽  
Cost Effective ◽  
Complete Removal ◽  
Level Of Evidence ◽  
Invasive Approach ◽  
Pneumatic Lithotripsy ◽  
Novel Approach ◽  
Surgical Methods ◽  
Salivary Gland Stones

Objective: The sialendoscopy era in the treatment of salivary gland stones has reduced the use of classical surgical methods. However, the miniature ducts and tools may cause difficulties in removing large sialoliths. Therefore, invasive combined oral surgeries or gland resection may be considered. We searched for the most suitable method in order to stay in line with the minimally invasive approach that preserves the ductus anatomy, and that can reduce the surgical fears of patients. Materials and Methods: The study included 84 cases (23 parotid and 61 submandibular) in whom stones were fragmented by pneumatic lithotripsy and removed between January 2015 and January 2020. The parotid cases comprised 7 females and 16 males, and the submandibular cases comprised 25 females and 36 males. Intraductal lithotripsy was performed using pneumatic lithotripter. This study has fourth level of evidence. Results: Based on total number of cases (n = 84), success rate was 67/84 (79.7%) immediately after sialendoscopy, and overall success rate was 77/84 (91.6%). Based on number of stones treated (n = 111), our immediate success rate was 94/111 (84.6%), and overall success rate was 104/111 (93.7%). The success criteria were complete removal of the stone and fragments in a single sialendoscopy procedure and resolution of symptoms. Conclusions: We successfully treated salivary gland stones, including L3b stones, in our patient cohort with sialendoscopy combined with pneumatic lithotripsy. The lithotripsy method that we have adapted seems to be more useful and cost-effective compared to its alternatives. We were also able to preserve the ductus anatomy and relieve patients’ concerns. Level of Evidence: Level IV

scholarly journals Development of a Co-Dominant Cleaved Amplified Polymorphic Sequences Assay for the Rapid Detection and Differentiation of Two Pathogenic Clarireedia spp. Associated with Dollar Spot in Turfgrass

Agronomy ◽  
2021 ◽  
Vol 11 (8) ◽  
pp. 1489
Author(s):  
Tammy Stackhouse ◽  
Sumyya Waliullah ◽  
Alfredo D. Martinez-Espinoza ◽  
Bochra Bahri ◽  
Emran Ali
Keyword(s):  
Pcr Primers ◽  
The United States ◽  
Fungal Species ◽  
Dollar Spot ◽  
Direct Pcr ◽  
Specific Pcr ◽  
Specificity And Sensitivity ◽  
Pure Cultures ◽  
Speed Up ◽  
Cleaved Amplified Polymorphic Sequences

Dollar spot is one of the most destructive diseases in turfgrass. The causal agents belong to the genus Clarireedia, which are known for causing necrotic, sunken spots in turfgrass that coalesce into large damaged areas. In low tolerance settings like turfgrass, it is of vital importance to rapidly detect and identify the pathogens. There are a few methods available to identify the genus Clarireedia, but none of those are rapid enough and characterize down to the species level. This study produced a co-dominant cleaved amplified polymorphic sequences (CAPS) test that differentiates between C. jacksonii and C. monteithiana, the two species that cause dollar spot disease within the United States. The calmodulin gene (CaM) was targeted to generate Clarireedia spp. specific PCR primers. The CAPS assay was optimized and tested for specificity and sensitivity using DNA extracted from pure cultures of two Clarireedia spp. and other closely related fungal species. The results showed that the newly developed primer set could amplify both species and was highly sensitive as it detected DNA concentrations as low as 0.005 ng/µL. The assay was further validated using direct PCR to speed up the diagnosis process. This drastically reduces the time needed to identify the dollar spot pathogens. The resulting assay could be used throughout turfgrass settings for a rapid and precise identification method in the US.

BioWorma as an Aid for Controlling Ruminant Nematode Parasites

2021 ◽  
Vol 99 (Supplement_2) ◽  
pp. 36-36
Author(s):  
James E Miller ◽  
Joan M Burke ◽  
Thomas H Terrill
Keyword(s):  
Delivery System ◽  
Control Method ◽  
Animal Health ◽  
Late Pregnancy ◽  
Cost Effective ◽  
The United States ◽  
Gastrointestinal Nematodes ◽  
Mineral Supplement ◽  
Larval Stages ◽  
Nematode Parasites

Abstract Nematode-trapping fungi are biological control agents used against the larval stages of gastrointestinal nematodes in livestock feces. These fungi are normal soil inhabitants where they feed on a variety of non-parasitic soil nematodes. Of the various fungi tested, Duddingtonia flagrans spores (BioWorma, International Animal Health Products, Australia) have been shown to survive passage through the gastrointestinal tract of ruminants. After defecation, the spores germinate and grow in the feces to form sticky, sophisticated traps/loops which are able to trap the developing larval stages in the fecal environment. This form of control has been successfully applied under field conditions and is an environmentally safe biological approach for forage-based feeding systems. BioWorma has recently been approved for use in the United States. The primary delivery system is mixing BioWorma into supplement feedstuffs daily where each animal has the opportunity to consume an adequate amount of the mixture. To achieve optimum control of larvae during the transmission season (May–October), BioWorma needs to be fed for a period of no shorter than 60 days, starting at the beginning of the grazing season (especially young after weaning). Feeding BioWorma to dams during late pregnancy and lactation will also help to reduce pasture contamination, especially for growing young that will graze the same pasture. Another delivery system is mixing BioWorma into loose mineral supplement where animals will consume it free choice. The mineral needs to be kept covered and dry. The spores cannot be incorporated into pellets as the heat of the pelleting process will kill the spores. One drawback in using BioWorma is the relatively high cost. Research is being conducted to evaluate other delivery schemes that could be more cost effective. This product is the only control method that targets nematodes on pasture, where a majority of the total population reside.

Response to Dr Stone

PEDIATRICS ◽  
1995 ◽  
Vol 96 (4) ◽  
pp. 850-851
Author(s):  
Howard A. Pearson
Keyword(s):  
Nurse Practitioners ◽  
Cost Effective ◽  
The United States ◽  
Health Maintenance Organizations ◽  
Quality Health Care ◽  
Quality Cost ◽  
Health Maintenance ◽  
Effective Care ◽  
Quality Health

Dr Elsa Stone, in her usual organized and lucid fashion, has presented the case for inclusion of pediatric nurse practitioners (PNPs) in private pediatric practices. She bases her conclusions on her nearly 10 years of positive experience with a PNP in her own practice in Connecticut. Dr Stone describes the PNP population and demography, describes the training curriculum of PNPs, and discusses the scope of work of these individuals. She concludes that "there is substantial evidence that PNPs provide quality health care and that collaborative teams of pediatricians and PNPs can provide high-quality, cost-effective care to a broader spectrum of children than can be served by either profession alone." The American Academy of Pediatrics (AAP) has insisted for several years that there is a shortage of pediatricians to meet the expanding needs of the children of the United States. Furthermore, pediatricians—because of system changes—will be expected increasingly to provide a variety of time-intensive services. Dr Stone believes that many of these services can be well provided by PNPs. Within the AAP, there have been some concerns about the role of PNPs. Of particular worry seems to be the possibility that PNPs might decide to practice independently, leading to a lower quality of care for their patients. Less often stated, but clearly an issue, is that PNPs are viewed by some pediatricians as potential competitors. Dr Stone's demographic analysis of what PNPs are currently doing is relevant to these concerns. One third of PNPs work in private pediatric practices or health maintenance organizations.

QS-9000 – Using the Automotive Requirements to Improve Existing ISO 9000 Systems

1998 ◽  
Author(s):  
J.F. Young
Keyword(s):  
Continuous Improvement ◽  
Cost Effective ◽  
Iso 9000 ◽  
The United States ◽  
Quality System ◽  
General Motors ◽  
Improvement Program ◽  
Quality Initiatives ◽  
Cost Of Quality ◽  
Improvement Programs

Abstract In this paper, we will discuss how to incorporate several of the QS-9000 quality system requirements into an existing ISO 9000 quality system. This expansion of the existing quality system will be cost effective and ensure that the continuous improvement program will meet its goals. The QS-9000 quality system was developed in 1994 by the "Big Three" automotive and truck producers in Detroit, Michigan. This document, originated in the United States, is based upon the International Standard: ISO 9001-94. It is mandatory that any supplier of goods and/or services to either Chrysler, Ford or General Motors must have their quality system registered to the QS-9000 standard. We believe that there can be benefits to non-automotive organizations if they will incorporate some of the QS-9000 clauses into their current ISO 9000 quality systems. We will discuss actual cases in the following areas: purchasing, safety, process control, training, business management and continuous improvement. We will show how these examples of quality system enhancement are both effective and efficient ways of driving cost reduction and process improvement programs. Cost of quality initiatives can be better managed.

A Tool for Designing Policy Packages To Achieve India’s Climate Targets: Methods, Data, and Reference Scenario of the India Energy Policy Simulator

2021 ◽  
Author(s):  
Deepthi Swamy ◽  
Apurba Mitra ◽  
Varun Agarwal ◽  
Megan Mahajan ◽  
Robbie Orvis
Keyword(s):  
Energy Policy ◽  
Ghg Emissions ◽  
Cost Effective ◽  
The United States ◽  
Technical Note ◽  
Reference Scenario ◽  
Low Carbon ◽  
Climate Targets ◽  
Effective Manner ◽  
Business As Usual

India is currently the world’s third-largest emitter of greenhouse gases (GHGs) after China and the United States and is set to experience continued growth in its population, economy, and energy consumption. Exploring low-carbon development pathways for India is therefore crucial for achieving the goal of global decarbonization. India has pledged to reduce the emission intensity of its gross domestic product (GDP) by 33–35 per cent relative to 2005 levels by 2030 through its Nationally Determined Contribution (NDC), among other related targets for the renewable energy and forestry sectors. Further, countries, including India, are expected to respond to the invitation of the Conference of the Parties (COP) to the Paris Agreement to communicate new or updated NDCs with enhanced ambition and long-term low-GHG development strategies for 2050. To design effective policy packages to support the planning and achievement of such climate targets, policymakers need to identify policies that can reduce GHG emissions in a timely and cost-effective manner, while meeting development-related and other national objectives. The India Energy Policy Simulator (India EPS), an open-source, system dynamics model, can enable an integrated quantitative assessment of different cross-sectoral climate policy packages for India through 2050 and their implications for key variables of interest such as emissions, GDP, and jobs. The tool was developed by Energy Innovation LLC and adapted for India in partnership with World Resources Institute. It is available for open access through a Web interface as well as a downloadable application. This technical note describes the structure, input data sources, assumptions, and limitations of the India EPS, as well as the setup and key results of its reference scenario, referred to as the business-as-usual (BAU) scenario in the model. It is intended as an update to the first technical note on the India EPS (Mangan et al. 2019) and accounts for the changes incorporated into the model since the first version.

scholarly journals Surgical instrumentation: the true cost of instrument trays and a potential strategy for optimization

2015 ◽  
Vol 4 (6) ◽  
pp. 82 ◽  
Author(s):  
Julie M. Mhlaba ◽  
Emily W. Stockert ◽  
Martin Coronel ◽  
Alexander J. Langerman
Keyword(s):  
United States ◽  
Cost Savings ◽  
Cost Effective ◽  
The United States ◽  
Waste Reduction ◽  
Significant Cost ◽  
Instrument Type ◽  
Hospital Revenue ◽  
Potential Strategy ◽  
The Cost

Objective: Operating rooms (OR) generate a large portion of hospital revenue and waste. Consequently, improving efficiency and reducing waste is a high priority. Our objective was to quantify waste associated with opened but unused instruments from trays and to compare this with the cost of individually wrapping instruments.Methods: Data was collected from June to November of 2013 in a 550-bed hospital in the United States. We recorded the instrument usage of two commonly-used trays for ten cases each. The time to decontaminate and reassemble instrument trays and peel packs was measured, and the cost to reprocess one instrument was calculated.Results: Average utilization was 14% for the Plastic Soft Tissue Tray and 29% for the Major Laparotomy Tray. Of 98 instruments in the Plastics tray (n = 10), 0% was used in all cases observed and 59% were used in no observed cases. Of 110 instruments in the Major Tray (n = 10), 0% was used in all cases observed and 25% were used in no observed cases. Average cost to reprocess one instrument was $0.34-$0.47 in a tray and $0.81-$0.84 in a peel pack, or individually-wrapped instrument.Conclusions: We estimate that the cost of peel packing an instrument is roughly two times the cost of tray packing. Therefore, it becomes more cost effective from a processing standpoint to package an instrument in a peel pack when there is less than a 42%-56% probability of use depending on instrument type. This study demonstrates an opportunity for reorganization of instrument delivery that could result in a significant cost-savings and waste reduction.

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