scholarly journals POS0358 EVALUATION OF THE EFFECT OF mTOR EXPRESSION ON THE CLINICAL MANIFESTATIONS OF KNEE OSTEOARTHRITIS IN OBESE AND NORMAL WEIGHT PATIENTS

2021 ◽  
Vol 80 (Suppl 1) ◽  
pp. 409.2-409
Author(s):  
E. Strebkova ◽  
E. Tchetina ◽  
L. Alekseeva
Keyword(s):  
Body Weight ◽  
Knee Pain ◽  
Mammalian Target Of Rapamycin ◽  
Clinical Manifestations ◽  
Normal Weight ◽  
Womac Pain ◽  
Obese Patients ◽  
Normal Body ◽  
Knee Oa ◽  
Normal Body Weight

Background:Currently, a large number of molecular biological and genetic markers are known to be involved in the development of osteoarthritis (OA). The mammalian target of rapamycin (mTOR) signaling pathway is responsible for chondrocyte proliferation, cartilage matrix production, and cell growth. OA is characterized by increased mTOR synthesis, which is accompanied by an increase in proliferative activity and destruction of chondrocytes. Obesity is an important factor in the progression of knee OA. The study of mTOR expression in patients with OA and obesity is an urgent task in the development of personalized OA therapy.Objectives:To determine the expression of mTOR in patients with knee OA in combination with obesity and normal body weight. To evaluate the effect of mTOR on the clinical manifestations of OA in patients with different body mass index (BMI).Methods:The study included 73 female patients aged 45-65 y.o. with Kellgren-Lawrence stage II-III knee OA. The patients were divided into 2 groups: group 1 (n=50) with obesity (BMI > 30 kg / cm2) and group 2 (n=23) with normal or increased body weight (BMI < 30 kg/cm2). The average age of patients with obesity is 56.5 ± 5.87 years, without obesity - 58.7 ± 5.43 years. Clinical manifestations were evaluated by a WOMAС. RNA was isolated from the patients ‘ blood samples, which was used to determine the expression of mTOR.Results:Patients with knee OA with and without obesity did not differ in age. OA develops at an earlier age in obese patients, than in non-obese patients (p < 0.001). Patients from 1 group had a high BMI > 30 kg/m2 at the onset of OA. Obese patients had more severe knee OA is significantly more often detected: Kellgren-Lawrence stage III was determined in 10% of obese patients and in 4.35% - without obesity (p < 0.001). Significantly higher values of the WOMAC index pain, stiffness, joint functional failure, and total WOMAC were observed in obese patients (p = 0.006, p = 0.039, p = 0.037, and p = 0.014, respectively). Obese patients had higher VAS pain scores (p < 0.05) compared to patients with a lower BMI. Obese patients had a higher mTOR expression (p < 0.05) of 8.02±8.62, compared to non-obese patients. High mTOR expression was associated with VAS knee pain (r=0.78; p < 0.05) and WOMAC pain (r=0.89; p<0.05) in obese patients (Table 1).Table 1.Correlation of m-TORParametersmTOR (1 group, n=50)mTOR (2 group, n=23)Body weightр > 0,05р > 0,05Pain (VAS)r=0,78; р<0,05p = 0,07; r = 0,45Pain (WOMAC)r=0,89; р<0,05р > 0,05Total WOMACр > 0,05р > 0,05Conclusion:Our study showed that patients with obesity and knee OA have higher rates of mTOR expression, compared to patients with normal body weight. High mTOR expression correlates with the severity of knee pain in obese patients. Thus, the evaluation of mTOR expression in obese patients and knee OA plays an important role in predicting the severity of clinical manifestations of OA, and may influence the choice of personalized therapy tactics for such patients.Disclosure of Interests:None declared

Features of the course of acute pancreatitis in patients with obesity

2019 ◽  
Vol 91 (5) ◽  
pp. 1-5 ◽  
Author(s):  
Andriy Kebkalo ◽  
Olha Tkachuk ◽  
Adrian Reyti
Keyword(s):  
Acute Pancreatitis ◽  
Body Weight ◽  
Mortality Rate ◽  
The Body ◽  
Obese Patients ◽  
Correlation Relationship ◽  
Systemic Complications ◽  
Key Words Acute Pancreatitis ◽  
Normal Body ◽  
Normal Body Weight

Abstract The purpose of the study is to investigate the course of acute pancreatitis in obese patients, the development of local and systemic complications and mortality rates. Materials and methods We have taken and analyzed 482 histories of acute pancreatitis, who has been treatmented at Kyiv Regional Clinical Hospital from January 1, 2011 to February 2, 2019. The data were statistically processed in the Exel 2010 program using a descriptive method using relative , absolute numbers, mean square deviations and their errors. A correlation relationship between variables was studied using the Pearson criterion (R2). Testing the significance of the difference between the two independent groups was carried out with the help of the t-criterion of the Student. Results In our study we had 482 patients, 260 patients (54%) had obesity (the study group), for comparison, a control group of patients with normal body weight was chosen, the total number of which was 222 (46%) patients. Obesity patients had a higher average age (55.4 ± 9.4 years, p = 0.01), also they had statistically greater percentage of severe cases of acute pancreatitis (85 (32.7%) vs 16 (7.2%); p = 0.01 *). We note the increase in the percentage of the course of severe pancreatitis in patients with weight gain from 10.20% to 53.93% (p = 0.03) *. Obesity patients had the longer bed-days in the hospital than in patients with normal body weight. In addition, they observed an increase of twice the bed in the reanimation and intensive care unit (5.8 ± 0.8 vs 2.7 ± 0.5 days, p = 0.01 *). When investigating the mortality rate, it should be noted that the main cause of death was the progression of organ failure of 30 (6.3%), pulmonary embolism (TB) - 15 (3.1%) and DIC 18 (3.7%) . Conclusions. The presence of obesity in patients involves a high risk of severe acute pancreatitis, this risk increases with an increase in the body mass index. In addition, in obese patients we have a higher bed-day in-patient and in-patient-care settings, which increases the total cost of treatment and requires a cost-effective algorithm in the future. A high mortality rate in obese patients requires improved treatment algorithm. Key words: acute pancreatitis, obesity.

scholarly journals TRAUMATIC DISEASE PECULIARITIES COURSE, ITS DIAGNOSTICS AND TREATMENT AT POLYTRAUMA IN SUFFER OBESE PATIENTS

2019 ◽  
Vol 43 (4) ◽  
pp. 47-53
Author(s):  
S.D. Khimich ◽  
O. M. Chemerys
Keyword(s):  
Body Mass Index ◽  
Body Weight ◽  
Body Mass ◽  
Overweight And Obesity ◽  
The Body ◽  
Obese Patients ◽  
Prognostic Features ◽  
Normal Body ◽  
Significant Difference ◽  
Normal Body Weight

Abstract Introduction. It’s known that the issue of polytrauma is one of the most urgent problems of surgery, and among injured patients a special approach is required for patients with overweight and obesity of varying degrees. Purpose of the study. To study prognostic features of traumatic disease course and to improve the results of diagnostics and surgical treatment of patients with polytrauma suffer obesity. Materials and methods. Clinical material was made up of 106 patients with combined body trauma, which were divided into three groups according to body mass index. Results. The results of the research showed a significant difference in the course of traumatic disease in patients with normal body weight and obesity. In particular, in the process of diagnostics of blunt chest and abdominal trauma the frequency of application of interventional methods of diagnostics was directly proportional to the increase of body mass index. The course of traumatic disease in the obese patients had a number of characteristic features that formed the basis for the development of diagnostics and differential program of treatment. Conclusions. The results of the research showed that the course of traumatic disease in combined injury obese patients is directly proportional to the body mass index and has certain features that differentiate them from patients with normal body weight. Keywords: polytrauma, obesity, traumatic disease, diagnostics, treatment.

scholarly journals Levels of keratinocyteand fibroblast-derived chemokines in skin cells isolated from abdominoplasty surgical materials from patients with normal weight and obesity

2020 ◽  
pp. 47-53
Author(s):  
А.Е. Копасов ◽  
Е.Н. Волкова ◽  
С.Н. Блохин ◽  
С.Г. Морозов
Keyword(s):  
Flow Cytometry ◽  
Body Weight ◽  
Subcutaneous Fat ◽  
Normal Weight ◽  
Obese Patients ◽  
Becton Dickinson ◽  
Skin Cells ◽  
Inflammatory Chemokines ◽  
Normal Body Weight ◽  
Body Weight Index

Цель работы - определение уровня хемокинов, ассоциированных с фибробластами и кератиноцитами, в клетках кожи, выделеных из операционного материала при абдоминопластике у пациенток с нормальной массой тела и ожирением. Методика. Для исследования использовали фрагменты кожи пациенток с нормальной массой тела и ожирением. Степень ожирения оценивали по индексу массы тела (ИМТ) согласно международным критериям. ИМТ, принятый в исследовании за норму, составлял 19,8±1,7 кг/м2, для пациенток с ожирением - 38,3±4,1 кг/м2. Клетки выделяли путем ферментативной обработки коллагеназой II. Проводили иммунотипирование клеток моноклональными антителами меченными флуоресцентными красителями. Использовали антитела к хемокинам семейства CXCL (R&D systems) и CCL (Boeringer Ingelheim, Германия). Меченные флуоресцентными красителями иммунотипированные клетки анализировали на проточном цитометре FACSCalibur (Becton Dickinson, США) по программе SimulSet. Статистический анализ проводили по программе ANOVA. Результаты. Были проанализированы хемокины семейства CCL и CXCL, секретируемые кератиноцитами и фибробластами кожи. Показано, что в коже пациенток с ожирением повышен процент клеток, экспрссирующих рецепторы CXCR3, CXCR4, CCR3, CCR10, регулирующие секрецию хемокинов кератиноцитами и фибробластами кожи. При ожирении в клетках кожи повышен уровень хемокинов CXCL8, CXCL9, CXCL10, CXCL13, CCL24 и CCL27, имеющих отношение к развитию и поддержанию воспалительного процесса в коже, а уровень хемокинов CCL17, CCL22, CCL28 снижен, что указывает на нарушение хемокиновой и цитокиновой регуляции при ожирении и является основой для развития послеоперационных осложнений при абдоминопластике. Заключение. Полученные данные указывают на нарушение хемокиновой регуляции при ожирении, что способствует развитию послеоперационных осложнений при абдоминопластике. The aim of the study was to compare chemokine expression in skin cells obtained from patients with normal body weight and obesity after abdominoplasty. Methods. Obesity was determined by body weight index (normal, 19.8±1.7 kg/m2; obesity, 38.3±4.1 kg/m2). Only skin without subcutaneous fat was isolated from surgical material. The skin cells obtained from surgical material were stained with monoclonal antibodies to chemokines. Fluorescence of proteins and receptors was analyzed by flow cytometry. Statistical analysis was performed with ANOVA. Results. Keratinocyte- and fibroblast-derived chemokines of the CCL and CXCL families were analyzed. Percentage of cells expressing CXCR3, CXCR4, CCR3, and CCR10 receptors (regulators of chemokine secretion by keratinocytes and skin fibroblasts) was increased in the skin of obese patients. Expression of the pro-inflammatory chemokines, CXCL8, CXCL9, CXCL10, CXCL13, CCL24, and CCL27, was increased in skin cells from obese patients. Obesity was associated with reduced expression of the chemokines CCL17, CCL22, and CCL28 in skin cells. Conclusion. Chemokine regulation is disturbed in obesity, which may underlie the development of complications after abdominal surgery.

scholarly journals Adipokines level and insulin resistance in abdominally obese patients with acute ischemic non-lacunar hemispheric stroke

2019 ◽  
pp. 60-65
Author(s):  
M. Delva ◽  
N. Lytvynenko
Keyword(s):  
Insulin Resistance ◽  
Body Weight ◽  
Serum Level ◽  
Abdominal Obesity ◽  
Obese Patients ◽  
Normal Body ◽  
Hemispheric Stroke ◽  
Significant Difference ◽  
Normal Body Weight ◽  
Transient Elevation

It has been examined serum concentrations of adiponectin, leptin and insulin resistance HOMA-IR index among patients with abdominal obesity II class and normal body weight patients in the acute phase of ischemic non-lacunar (atherothrombotic and cardioembolic) hemispheric strokes. There was absence of any significant difference in adipokines concentration and insulin resistance in normal body weight patients with acute stroke. Under the conditions of acute ischemic non-lacunar hemispheric stroke, patients with abdominal obesity II class have sustained reduction of adiponectin serum level at 1st and 10th day after stroke and transient elevation of leptin serum level, as well as HOMA-IR index at 1st day with subsequent return to basal values on the 10th day. The extents of these neuroendocrine changes in abdominally obese patients have a direct association with cerebral infarction volumes. These neuroendocrine peculiarities may be, at least partly, one reason of more severe stroke in abdominally obese patients.

scholarly journals Cardiopulmonary Capacity in Overweight and Obese Children and Adolescents: A Cross-Sectional Study

2021 ◽  
Vol 12 ◽  
Author(s):  
Agata Grzyb ◽  
Małgorzata Domagalska-Szopa ◽  
Andrzej Siwiec ◽  
Ilona Kwiecień-Czerwieniec ◽  
Andrzej Szopa
Keyword(s):  
Body Weight ◽  
Children And Adolescents ◽  
Cardiopulmonary Exercise Testing ◽  
High Body Mass Index ◽  
Normal Weight ◽  
Control Group ◽  
Overweight Children ◽  
Obese Children ◽  
Normal Body ◽  
Normal Body Weight

Background: One of the objective methods of assessing the level of cardiopulmonary capacity in overweight and obese children and adolescents is cardiopulmonary exercise testing (CPET).Aims: The purpose of present study is an evaluation of aerobic capacity in high body mass index (BMI) children and adolescents by comparing them with a normal weight control group by CPET.Methods and Procedures: The subjects were recruited from participants of the Program of Treatment for Overweight and Obese Children organized by a local pediatric rehabilitation center in Poland. Based on BMI for age and gender, two validation groups were selected: (1) a group of overweight children (n = 49) and (2) a group of obese children (n = 48). The study included also 53 normal weight participants as a reference group (REF). The study consisted of two parts: anthropometric measurements and CPET. The Godfrey protocol for CPET was applied.Outcomes and Results: In this study, obese children and adolescents showed similar absolute VO2peak values in liters per minute (1.64 L/min) compared to overweight children (1.48 L/min), but significantly higher than children with normal body weight (1.39 L/min). The obese children and adolescents presented lower VO2peak in relation to body weight (25.44 ml/kg/min) compared to their peers with normal body weight (36.5 ml/kg/min), and overweight children (29.18 ml/kg/min).Conclusion and Implications: The main finding of our study was recognition of significant differences between cardiopulmonary capacity parameters in obese children in comparison not only to normal weight peers, but to overweight, too.

scholarly journals The association of positive mental health indicators and normal body weight, Belgian HIS 2018

2020 ◽  
Vol 30 (Supplement_5) ◽  
Author(s):  
K De Ridder ◽  
S Drieskens ◽  
S Demarest
Keyword(s):  
Mental Health ◽  
Life Satisfaction ◽  
Body Weight ◽  
Psychological Distress ◽  
Health Indicators ◽  
Normal Weight ◽  
Positive Mental Health ◽  
Normal Body ◽  
Normal Body Weight ◽  
Low Educated

Abstract Background An important health promotion goal is to maintain a normal body weight. However, knowledge about positive mental health indicators that might be associated with normal weight is scarce. The aim is to study positive mental health indicators and normal weight in a cross-sectional national representative population sample. Methods Study participants included adults aged ≥ 18 year (n = 6643) of the Belgian Health Interview Survey (HIS) 2018. Mental health indicators were having no psychological distress (GHQ-12&lt;2), no self-reported anxiety or depression (EQ_5D), medium to high vitality (SF-36 vitality scale) and high to medium level of life satisfaction. BMI was calculated on self-declared height and weight. The associations were assessed through logistic regression taking into account confounding and effect modification by age, gender, educational level, health status, physical activity, daily eating of fruit and vegetables and daily consuming sugared soft drinks or sweet/salty snacks. Results 50.1% had a BMI≥25. In the crude models, all indicators were negatively associated with being obese, but there was no association between having a normal weight (versus overweight and obesity) and absence of psychological distress, absence of anxiety and depression, and medium to high vitality. Medium to high level of life satisfaction was associated with normal weight in the fully adjusted model (OR 1.3; 95%CI 1.0-1.6) but when stratified, medium to high life satisfaction was associated with normal weight only among high educated women (OR 1.8; 95%CI 1.1-2.9), but not among men and low educated women. Surprisingly, among low educated women, having no psychological distress was negatively associated with normal weight (OR 0.7; 95%CI 0.5-0.9). Conclusions These preliminary results showed a variable impact of gender and educational levels on the association between positive mental health indicators and normal weight. Trends and distribution in society should be further explored. Key messages Although unequivocal associations with obesity, positive mental health is not clearly associated with normal body weight in today’s obesogenic society. The effect of a shift towards a higher BMI in society on mental health needs to be further explored.

scholarly journals POS1090 ASSOCIATION BETWEEN ANTIBIOTIC USE AND KNEE PAIN AND FUNCTION IN OA: DATA FROM THE OSTEOARTHRITIS INITIATIVE

2021 ◽  
Vol 80 (Suppl 1) ◽  
pp. 824.2-825
Author(s):  
I. Shirinsky ◽  
V. Zagainova ◽  
V. Shirinsky
Keyword(s):  
Knee Pain ◽  
Joint Pain ◽  
Clinical Manifestations ◽  
Antibiotic Use ◽  
Womac Pain ◽  
Knee Oa ◽  
Link Type ◽  
Time Interaction ◽  
Osteoarthritis Initiative ◽  
And Function

Background:Recently, gut dysbiosis has been linked with joint pain in osteoarthritis (OA) (1). Thus, it can be hypothesized that antibiotic use impacts symptoms of OA. To date, there have been no studies assessing association between the intake of antibiotics and clinical manifestations of knee OA.Objectives:To evaluate association between the use of antibiotics and OA-related knee pain and function.Methods:For the current study we used 4-year longitudinal data obtained from the Osteoarthritis Initiative (OAI) progression (n= 1390) and incidence (n = 3284) subcohorts, which are publically available at https://oai.nih.gov. The outcome variables studied were Western Ontario McMaster Osteoarthritis Index (WOMAC) pain and function subscales. To acquire information about the use of antibiotics, a medication inventory method was used. We excluded participants who used antibiotics at or 30 days prior to baseline (a “new-user” design). Antibiotic users were defined as those with at least one recorded use during first four years of the study. The participants were matched into antibiotic users/non-users pairs using genetic matching based on various demographic and clinical characteristics. The outcomes were assessed at baseline and annually until year 4. Generalized estimating equations (GEE) were used to model the relationship between outcomes and antibiotic use by time interaction.Results:A total of 515 new antibiotic users were matched with 515 non-users. After matching, all standardized differences of means were less than 0.05 indicating that covariates were well balanced between groups. No association between the use of antibiotic and changes in WOMAC pain and function scores was found (Table 1). No associations were also observed in multiple stratified analyses based on different duration of antibiotic use, particular classes of antibiotics, or different baseline WOMAC pain or disability levels.Table 1.Longitudinal analysis (with treatment*time interaction) of WOMAC subcategories and antibiotics useβ coefficient95% CIP valueWOMAC Pain-0.008(-0.08 to 0.07)0.8WOMAC Function-0.014(-0.24 to 0.21)0.9Conclusion:These findings do not support the effects of antibiotics on knee OA pain or disability. More research is needed on the role of microbiota and its modulation in OA.References:[1]Boer, C.G., Radjabzadeh, D., Medina-Gomez, C. et al. Intestinal microbiome composition and its relation to joint pain and inflammation. Nat Commun 10, 4881 (2019). https://doi.org/10.1038/s41467-019-12873-4Disclosure of Interests:None declared.

scholarly journals INFLUENCE OF HYGIENIC AND DIETARY HABITS ON INCIDENCE OF DENTAL CARIES IN YOUNG NORMAL-WEIGHT AND OVERWEIGHT SCHOOLCHILDREN

2019 ◽  
Vol 19 (4) ◽  
pp. 77-82
Author(s):  
O.V. Sheshukova ◽  
A. V. Veretilnik
Keyword(s):  
Body Weight ◽  
Dental Caries ◽  
Dietary Habits ◽  
Normal Weight ◽  
Permanent Teeth ◽  
Overweight Children ◽  
Normal Body ◽  
Dental Examination ◽  
Significant Difference ◽  
Normal Body Weight

This paper presents the analysis of foreign and domestic literature on the current state of the epidemic of obesity and evidence of its association with oral disease. The aim of this study was to assess the hygienic and dietary habits of overweight and normal-weight young schoolchildren and their association with dental caries. Materials and methods. The study included 628 children aged 6-11 years studying at primary schools of Poltava. We measured anthropometric data, assessed physical development, collected anamnestic data of children and carried out clinical dental examination. The oral hygienic state was assessed by using the simplified hygienic Green-Vermillion index (1964). Results. According to the findings of anthropometric survey obtained, we found out that 32, 64% (205 children) of the children were overweight. Having analyzed the data obtained by the questionnaire, it is safe to say that there is no noticeable difference in the hygienic habits between the primary schoolchildren involved in the study. Only about 70% of them brush their teeth every day, but only 15% of children get assistance from their parents or siblings. Such data are alarming due to the fact that as young children usually cannot clean their teeth properly therefore children under 10 should be under the control or guidance of their parents when brushing the teeth. Dietary habits of the overweight and normal-weight children are significantly different. The index of hygiene in children with excess body weight was within 2.1 scores that corresponds to unsatisfactory hygiene; children with normal body weight demonstrated significantly lower indicators ranging within 1.8 scores. The results of the dental examination showed that the caries prevalence was 60.78% and 58.63% for children with normal weight and excessive weight, respectively. Those figures did not differ significantly from the indicated age group of children with excessive and normal body weight. The study of the caries intensity showed that in the normal-weight children the df + DMF index was 1.89 ± 0.01, and the overweight children had the df + DMF 1.82 ± 0.04 index: thus, no significant difference was revealed. Caries of temporary teeth in children with excessive body weight was significantly seldom detected than in the group of children with normal body weight (2.46 ± 0.05 and 2.82 ± 0.02, respectively). The results of examining children with overweight and obesity demonstrated significantly lower intensity index of complicated caries of temporary teeth (1.07 ± 0.04 and 1.28 ± 0.02, respectively). Conclusions. Consequently, there is no significant difference in the hygienic habits between normal-weight and overweight children. As for dietary habits, there is a significant difference revealed by questioning the children. There is no difference in the hygienic index in the children of these two groups. The prevalence of caries was within the normal limits. A significant difference has been found in determining the caries intensity of permanent teeth in young school children of the studied groups. It is noteworthy that lower rates of caries intensity and its complications affecting temporary teeth have been found out in the overweight children when compared with peers having normal body weight; the patterns in the growth of the caries intensity for permanent teeth in the overweight children have been determined as well. Mostly due to inadequate hygiene, lack of balanced nutrition and adherence to a diet rich in carbohydrates, young children are especially vulnerable category of dental patients.

scholarly journals The mTOR kinase and its associated proteins in skin cells isolated during abdominoplasty in patients with normal body weight and obesity

2020 ◽  
pp. 40-46
Author(s):  
С.Г. Морозов ◽  
А.Е. Копасов
Keyword(s):  
Body Weight ◽  
Signaling Pathways ◽  
Complete Medium ◽  
Obese Patients ◽  
Kinase Signaling ◽  
Normal Body ◽  
Skin Cells ◽  
Mtor Kinase ◽  
Associated Proteins ◽  
Normal Body Weight

Цель работы - измерение уровня белков, ассоциированных с сигнальными путями киназы mTOR (mTOR, Raptor, Rictor, 4Е-ВР1 p70-S6K1, AKT) в клетках кожи выделенных при операции абдоминопластики у пациентов с нормальной массой тела и ожирением. Основное значение исследования - установление участия сигнальных путей киназы mTOR в патогенетических процессах при ожирении и их роли в развитии послеоперационных осложнений при операции абдоминопластики. Методика. Клетки кожи выделяли из операционного материала ферментативной обработкой, затем восстанавливали в полной среде, после чего проводили иммунотипирование маркеров рецепторов и внутриклеточных белков моноклональнми антителами меченными флуоресцентными красителями Интенсивность флуоресценции анализировали на проточном цитометре. Результаты. Проведено сравнение уровня белков, связанных с сигнальными путями киназы mTOR, в выделенных после абдоминопластики клетках кожи при ожирении и нормальной массе тела. Было установлено повышение уровня белков, ассоциированных с киназой mTOR, в клетках кожи пациенток с ожирением. Показано при ожирении преимущественное участие первого комплекса mTORC1 в активации клеток. Установлено повышение активности вторичных мессенджеров киназы mTOR - фактора 4E-BP1 и рибосомальной киназы p70-S6K1, передающих сигналы на рибосомы для синтеза белка в фибробластах и кератиноцитах кожи пациентов с ожирением после абдоминопластики. Заключение. Полученные данные отражают патогенетические особенности ожирения, что может иметь значение в развитии послеоперационных осложнений, наблюдаемых у пациенток с ожирением после абдоминопластики. Aim. To measure expression of kinase mTOR-associated proteins (mTOR, Raptor, Rictor, 4E-BP1 p70-S6K1, AKT) in skin cells isolated from patients with normal body weight and obesity after abdominoplasty. The importance of this study was in determining the role of mTOR kinase signaling pathways in the pathogenesis of obesity and obesity-associated complications after abdominal surgery. Methods. Skin cells were isolated from surgical material by enzymic treatment, restored in a complete medium, and then stained with monoclonal antibodies to receptors and intracellular proteins. The fluorescence intensity was analyzed with a flow cytometer. Results. Expression of proteins associated with mTOR kinase signaling pathways was compared in isolated skin cells from patients with obesity and normal body weight. Increased mTOR protein expression was found to be associated with obesity. The first mTORC1 complex primarily contributed to the activation of skin cells from obese patients after abdominoplasty. Activities of mTOR second messengers, 4E-BP1 kinase and ribosomal kinase p70-S6K1, which transmit signals to ribosomes for protein synthesis, were elevated in skin fibroblasts and keratinocytes of patients with obesity after abdominoplasty. Conclusion. Results of this study delineated features of the pathogenesis of obesity that may be related with postoperative complications of abdominoplasty typical for obese patients.

Anorexia Nervosa at Normal Body Weight!–The Abnormal Normal Weight Control Syndrome

1982 ◽  
Vol 11 (3) ◽  
pp. 203-233 ◽  
Author(s):  
A. H. Crisp
Keyword(s):  
Body Weight ◽  
Anorexia Nervosa ◽  
Weight Control ◽  
Eating Behaviour ◽  
Normal Weight ◽  
Normal Body ◽  
Self Mutilation ◽  
Normal Body Weight ◽  
Body Weight Control ◽  
Normal Weight Control

Disgust with ‘fatness' and a consequent preoccupation with body weight, coupled with an inability to reduce it to or sustain it at the desired low level, characterizes the abnormal normal weight control syndrome. Individuals remain sexually active in a biological sense and often also socially. Indeed their sexual behaviour may be as impulse ridden as is their eating behaviour, which often comprises phases of massive bingeing coupled with vomiting and/or purgation. The syndrome is unlike frank anorexia nervosa in that the latter involves a regression to a position of phobic avoidance of normal body weight and consequent low body weight control with inhibition of both biological and social sexual activity. In abnormal normal weight control there is a strong and sometimes desperate hedonistic and extrovert element that will often not be denied so long as body weight does not get too low. Individuals nevertheless feel desperately ‘out of control’ and insecure beneath their bravura. The syndrome is much more common in females than in males. There is a clinical overlap with anorexia nervosa and obesity in many cases as the disorder evolves. Depression, stealing, drug dependence (including alcohol) and acute self-poisoning and self-mutilation are common complications. Clinic cases probably only represent the tip of the iceberg of a much more widespread morbidity within the general population. Like anorexia nervosa and for the same reasons the disorder is probably more common than it used to be.

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