Rhizoctonia fungi enhance the growth of the endangered orchid Cymbidium goeringii

Botany ◽  
2010 ◽  
Vol 88 (1) ◽  
pp. 20-29 ◽  
Author(s):  
Jianrong Wu ◽  
Huancheng Ma ◽  
Mei Lü ◽  
Sufen Han ◽  
Youyong Zhu ◽  
...  

Orchids are among the most prized ornamental plants in many societies throughout the world. As a result, consumer demands have created a significant pressure on wild populations of many species, including Cymbidium goeringii Rchb. f., a rare terrestrial orchid endemic in China, Korea, and Japan. To help conserve natural populations of C. goeringii, we recently started investigating methods to cultivate these orchids. Here we fulfilled Koch’s postulates and demonstrated that fungal strains isolated from the roots of natural Cymbidium plants increased fresh mass, plant height, number of leaves, and root length of C. goeringii, and that the two fungal strains originally isolated from C. goeringii showed overall greater effects on growth than two other strains from other Cymbidium species. Internal transcribed spacer sequence analyses revealed that the four fungal strains likely represented at least two new taxonomic groups, both belonging to the family Ceratobasidiaceae of the Rhizoctonia fungi. Our study demonstrated that these fungal strains could potentially help the commercial cultivation of the increasingly rare and endangered orchid C. goeringii.

2021 ◽  
pp. 1-4
Author(s):  
Yu-Wei Tseng ◽  
Chi-Chun Huang ◽  
Chih-Chiang Wang ◽  
Chiuan-Yu Li ◽  
Kuo-Hsiang Hung

Abstract Epilobium belongs to the family Onagraceae, which consists of approximately 200 species distributed worldwide, and some species have been used as medicinal plants. Epilobium nankotaizanense is an endemic and endangered herb that grows in the high mountains in Taiwan at an elevation of more than 3300 m. Alpine herbs are severely threatened by climate change, which leads to a reduction in their habitats and population sizes. However, only a few studies have addressed genetic diversity and population genetics. In the present study, we developed a new set of microsatellite markers for E. nankotaizanense using high-throughput genome sequencing data. Twenty polymorphic microsatellite markers were developed and tested on 30 individuals collected from three natural populations. These loci were successfully amplified, and polymorphisms were observed in E. nankotaizanense. The number of alleles per locus (A) ranged from 2.000 to 3.000, and the observed (Ho) and expected (He) heterozygosities ranged from 0.000 to 0.929 and from 0.034 to 0.631, respectively. The developed polymorphic microsatellite markers will be useful in future conservation genetic studies of E. nankotaizanense as well as for developing an effective conservation strategy for this species and facilitating germplasm collections and sustainable utilization of other Epilobium species.


2022 ◽  
Vol 4 (1) ◽  
pp. 001-012
Author(s):  
Carlos Henrique Marchiori

Simuliidae belong to the order Diptera, suborder Nematocera, Infraorder Culicomorpha, Superfamily Chironomoidea, and Family Simuliidae. They are known as “borrachudo” or “pium” in Brazil and as “black flies” in English-speaking countries. This study objective to report the characteristics of the Family Simuliidae. The research was carried out in studies related to quantitative aspects of the Family, Subfamily and Species (taxonomic groups) and conceptual aspects such as: biology, geographical distribution, species, life cycle, damage, economic importance, medicinal importance, biological aspects, and reproduction. A literature search was carried out containing articles published from 1950 to 2021. The mini-review was prepared in Goiânia, Goiás, from September to October 2021, through the Portal of Scientific Journals in Health Sciences, Pubmed, Online Scientific Library (Scielo), internet, ResearchGate, Academia.edu, Frontiers, Biological Abstract, Publons, Qeios, Dialnet, World, Wide Science, Springer, RefSeek, Microsoft Academic, Science, ERIC, Science Research.com, SEEK education, Periodicals CAPES, Google Academic, Bioline International and VADLO.


Author(s):  
Canan Nilay Duran ◽  
Gizem Demirkaplan ◽  
Sevinç Şener

Passionflower is a member of Passifloraceae family, it can be used as medicinal and ornamental plants in addition to its consumption as fruit in the world. Guava (Psidium guajava L.) fruits, which has a rich nutrient content, are consumed both fresh and processed. Passionfruit and guava plants, which can only be cultivated in temperate southern coasts in our country, have gained commercial importance because of the fruits' export potential, high medical importance and nutrient. This study was carried out to determine the effects of some bioactivator applications on the criteria of sapling growth and development of passionflower and guava plants. The experiment was conducted between 2018-2019 under greenhouse conditions. Saplings obtained from seed germination in Akdeniz University Faculty of Agriculture Research and Experiment Area were used as plant material. 3 different commercial preparations called Messenger, Crop-set and ISR-2000 were used as bioactivators. Plant height (cm), stem diameter (mm) and number of leaves (number / plant) were measured weekly, in order to determine the effect of the applications on the growth and development of saplings. At the end of the study, it was determined that the highest average plant height (10.17 cm), stem diameter (13.53 mm) were obtained in ISR-2000 application. The highest average plant height (11.93 cm), stem diameter (16.44 mm) and number of leaves (9.07 units / plant) were obtained from Messenger application in guava plant. When the results obtained are evaluated, it is recommended that ISR-2000 bioactivator can be applied in passionflower sapling cultivation and Messenger bioactivator can be applied for guava sapling cultivation.


2016 ◽  
Vol 2 (3) ◽  
pp. 22-28 ◽  
Author(s):  
Syifaur Rahmah ◽  
Ni Made Armini Wiendi ◽  
Willy B. Suwarno ◽  
Krisantini Krisantini

Dendrobium mannii and Dendrobium mirbelianum were rare and endangered orchid species. In vitro micro propagation could be used to conserve and proliferate these species. The aims of this study were to determine the effects of chitosan on proliferation of the protocorm-like-bodies (PLBs) of in vitro grown Dendrobium mannii and D. mirbelianum. Chitosan has been reported as an important component to promote orchid growth in vitro. In this study chitosan was included in the in-vitro media at the concentrations of 0, 1, 2, 3, and 4 mg.L-1. The highest PLBs formation, clump diameter, and fresh weight on D. mannii were obtained from explants cultured on medium supplemented with 1 mg.L-1 chitosan. After 14 weeks of culture, fresh weight, clump diameter and average number of PLBs of D.mannii were 29.52 %, 33.00 % and 35.88% greater at chitosan (1 mg.L-1) compared to control media.  D. mirbelianum treated with low chitosan concentrations (1 and 2 mg.L-1) had 65.71% more leaves than those without chitosan treatment. Chitosan, however, had an adverse effects on growth of both species at  4 mg.L-1. Medium contain 4 mg.L-1 chitosan induced PLBs proliferation of D.mannii, but inhibited PLBs proliferation of D.mirbelianum.  These results suggested that chitosan had important roles in PLB proliferation and could be used for the propagation of D. mannii PLBs in vitro. Keywords: rare orchid species, in-vitro


2021 ◽  
Vol 3 (6) ◽  
pp. 44-49
Author(s):  
Thị Hải Nguyễn ◽  
Huy Thái Trần ◽  
Thế Cường Nguyễn ◽  
Thị Thanh Vân Trần

In Na Hang Nature Reserve there was identified 647 medicinal plant species belonging to 137 families, 4 vascular divisions: Lycopodiophyta with 3 species, 2 families; Polypodiophyta with20 species, 12 families; Pinophyta with 6 species, 4 families; Magnoliophyta with 618 species, 119 families. Concerning the diversity index: the family index: 4.72; the genus index: 1.49; the genus index/the family index: 3.16. In Na Hang Nature Rserve 10 families more species occupying 7.30% of the total families of medicinal plants with the total of species occupying 31.68% and 28.64% of the total of genus. The family more diverse is Asteraceae with 32 species, occupying 4.95% of the total of medicinal plants identified; following is Rubiaceae with 28 species (4.33%), Euphorbiaceae with 26 species (4.02%); Moraceae with 23 species (3.55%); Cucurbitaceae, Verbenaceae with the same number of species 18 (2.78%); Araceae with 17 species (2.63%); Zingiberaceae with 16 species (2.47%); Urticaceae with 15 species (2.32%); and the last are families Poaceae of species 12 (1.85%) in the total of species. 10 genera more diverse with 59 species (9.12% of the total of medicinal plants), Ficus (Moraceae) is more diverse with 15 species (2.32% of the total of medicinal plants) and another genera with from 4 to 7 species. In Nature Reserve of Na Hang we have confirmed the distribution of 30 precious and rare and endangered species priorto the conservation: 23 species in Vietnam Red Data Book (2007): VU - 16 species, EN - 07 species; 17 species in Red List of medicinal plant of Vietnam (2006):VU - 10 species, EN - 6 species and CR – 01 species; 07 species in IUCN Red List (2014): LR - 06 species and VU – 01 species.


2017 ◽  
Vol 19 (2) ◽  
pp. 114 ◽  
Author(s):  
K. Trencheva ◽  
G. Trenchev ◽  
R. Tomov ◽  
S.-A. Wu

A preliminary list of non-indigenous scale insect species on ornamental plants in Bulgaria and China is presented. The sampling was done between April and November, 2009, in the framework of the project “Invasive scale insects on ornamental plants in Bulgaria and China”. The insects were collected in nurseries, parks, gardens, botanical collections and greenhouses. Representatives from four families have been identified in Bulgaria, the most numerous of which are the Diaspididae (eight species), Coccidae (four species), Pseudococcidae (two species) and Margarodidae (one species). Three species of non-indigenous scale insects associated with ornamental plants were collected in China, all belonging to the family Pseudococcidae. A list of alien scale insect species on ornamental plants is given, including the sampling sites, host plants on which they were found, origin and first report in both countries.


2013 ◽  
Vol 79 (15) ◽  
pp. 4520-4527 ◽  
Author(s):  
Yasuhito Okuda ◽  
Shigeyuki Murakami ◽  
Yoichi Honda ◽  
Teruyuki Matsumoto

ABSTRACTThe enormous number of spores produced by fruiting bodies during cultivation of mushrooms can lead to allergic reactions of workers, reduction of commercial value, spread of mushroom disease, pollution of facilities, and depletion of genetic diversity in natural populations. A cultivar harboring a sporulation-deficient (sporeless) mutation would be very useful for preventing these problems, but sporeless commercial cultivars are very limited in usefulness because sporeless traits are often linked with traits that are unfavorable for commercial cultivation. Thus, identifying a causal gene of a sporeless phenotype not linked to the adverse traits in breeding and cultivation is crucial for the establishment of sporeless breeding using a strategy employing targeting induced local lesions in genomes (TILLING) in cultivated mushrooms. We used aPleurotus pulmonarius(Fr.) Quél. sporeless strain to identify and characterize the single recessive gene controlling the mutation. The 3,853-bpstpp1gene encodes a protein of 854 amino acids and belongs to the MutS homolog (MSH) family associated with mismatch repair in DNA synthesis or recombination in meiosis. Gene expression analysis of the fruiting body showed that this gene is strongly expressed in the gills. Phenotypic analysis of disruptants formed by gene targeting suggested a reproducible sporeless phenotype. Mutants deficient in a functional copy of this gene have no unfavorable traits for sporeless cultivar breeding, so this gene will be an extremely useful target for efficient and versatile sporeless breeding inP. pulmonariusand various other cultivated mushrooms.


2021 ◽  
Vol 2 (1) ◽  
pp. 047-080
Author(s):  
Carlos Henrique Marchiori

Fruit flies belong to the Tephritidae Family which is one of the largest within the Order Diptera. This family is among the pests with the greatest economic expression in the world's fruit industry, as they attack the reproductive organs of plants, fruits with pulp and flowers. These insects are an important group of pests in the fruit industry worldwide, as they have a life cycle in which their larval period develops especially inside the fruits, feeding, in general, on their pulp. The objective of this study is to report the Ecology and Biology of Tephitidae. The research was carried out in studies related to quantitative aspects of the Family, Subfamily and Species (taxonomic groups) and conceptual aspects such as: biology, geographical distribution, methodologies, and traps for collecting and their parasitoids and strategies for Drosophlidae with control, species, life cycle, damage, economic importance, medicinal importance, biological aspects, monitoring and control and reproduction. A literature search was carried out containing articles published from 1993 to 2021. The mini-review was prepared in Goiânia, Goiás, from September to October 2021, through the. The mini-review was prepared in Goiânia, Goiás, from September to October 2021, through the Online Scientific Library (Scielo), internet, ResearchGate, Academia.edu, Frontiers, Publons, Qeios, Portal of Scientific Journals in Health Sciences, Pubmed, Online Scientific Library (Scielo), internet, ResearchGate, Academia.edu, Frontiers, Biological Abstract, Publons, Qeios, Portal of Scientific Journals in Health Sciences, and Pubmed, Dialnet, World, Wide Science, Springer, RefSeek, Microsoft Academic, Science, ERIC, Science Research.com, SEEK education, Periódicos CAPES, Google Academic, Bioline International and VADLO.


Zootaxa ◽  
2008 ◽  
Vol 1874 (1) ◽  
pp. 16 ◽  
Author(s):  
MARTIN FIKÁČEK ◽  
MIGUEL ARCHANGELSKY ◽  
PATRICIA L. M. TORRES

The primary chaetotaxy of the larval head capsule and head appendages of the family Hydrophilidae (Insecta: Coleoptera) is described and illustrated using the larva of Hydrobius fuscipes (Linnaeus, 1758) as a model, and compared with fifteen hydrophilid taxa representing all main taxonomic groups within the family; brief comparative notes with representatives of the families Helophoridae, Spercheidae, Hydrochidae and Histeridae are also provided. Primary chaetotaxic nomenclature is developed for the Hydrophilidae, allowing the use of chaetotaxic characters for phylogenetic studies as well as diagnostic purposes. The study of representatives of the families Helophoridae, Hydrochidae and Spercheidae suggests that this nomenclature can also be effectively applied to other hydrophiloid families. Chaetotaxic nomenclature systems used in larvae of other groups of Coleoptera are briefly reviewed.


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