Effects of conservation method on condensed tannin content, ruminal degradation, and in vitro intestinal digestion of purple prairie clover (Dalea purpurea Vent.)

2016 ◽  
Vol 96 (4) ◽  
pp. 524-531 ◽  
Author(s):  
Q.Q. Huang ◽  
L. Jin ◽  
Z. Xu ◽  
S. Acharya ◽  
T.A. McAllister ◽  
...  
Keyword(s):  
Crude Protein ◽  
Condensed Tannin ◽  
Neutral Detergent Fiber ◽  
Tannin Content ◽  
Freeze Dried ◽  
Intestinal Digestion ◽  
Ruminal Degradation ◽  
Conservation Method

In situ and in vitro experiments were conducted to evaluate the effect of forage conservation method on the chemical composition, ruminal degradation, and intestinal digestion of purple prairie clover (PPC), which was conserved as freeze-dried forage (FD), silage (SIL), or hay (Hay). In situ dry matter (DM), neutral detergent fiber (NDF), and crude protein (CP) degradabilities were determined by incubating the forages in three rumen-cannulated heifers for 0, 1, 2, 4, 8, 24, 48, and 72 h. Intestinal DM and CP digestions were estimated by incubating 12 h ruminal in situ residues in a modified three-step in vitro procedure. Ensiling decreased (P < 0.001) extractable condensed tannins (CT) but increased (P < 0.001) protein- and fiber-bound CT compared with FD and Hay. The ruminal disappearance of CP at 8 and 24 h was affected by conservation method (P < 0.001), ranked as SIL > FD > Hay. The effective degradability of DM was lower (P < 0.001) for Hay than for FD and SIL. Ensiling lowered (P < 0.001) whereas haymaking increased (P < 0.001) intestinal digestion of CP compared with the fresh PPC. There were no differences in intestinal DM digestion among the three conserved forages. The results suggest that PPC conserved as Hay may conserve the biological activity of CT via preserving extractable CT more than as SIL, thus having the potential to improve protein utilization in ruminants.

scholarly journals Evaluation of the Nutrient Composition, In Vitro Fermentation Characteristics, and In Situ Degradability of Amaranthus caudatus, Amaranthus cruentus, and Amaranthus hypochondriacus in Cattle

Animals ◽  
2020 ◽  
Vol 11 (1) ◽  
pp. 18
Author(s):  
Kim Margarette C. Nogoy ◽  
Jia Yu ◽  
Young Gyu Song ◽  
Shida Li ◽  
Jong-Wook Chung ◽  
...  
Keyword(s):  
Crude Protein ◽  
Dry Matter ◽  
Gas Production ◽  
Nutrient Composition ◽  
Neutral Detergent Fiber ◽  
Amaranthus Cruentus ◽  
Amaranthus Caudatus ◽  
Amaranthus Hypochondriacus

The amaranth plants showed high potential feed value as forage for ruminants. An in-depth study of this plant, particularly in cattle, will help extend its utilization as an alternative protein and fiber feed source in cattle feeding. In this study, the nutrient compositions of three different species of amaranth, Amaranthus caudatus L., Amaranthus cruentus L., and Amaranthus hypochondriacus L.—two varieties for each species, A.ca 74, A.ca 91, A.cu 62, A.cu 66, A. hy 30, and A. hy 48—were evaluated. The in vitro technique was used to evaluate the fermentation characteristics such as total gas production, total volatile fatty acids (VFA) concentration, pH, and ammonia concentration of the rumen fluid. Moreover, the effective degradabilities of dry matter (EDDM) and crude protein (EDCP) of the amaranth forages were determined through in situ bag technique. The amaranth forages: A. caudatus, A. cruentus, and A. hypochondriacus showed better nutritive value than the locally produced forages in Chungcheong province of Korea. The CP of the amaranth ranged from 11.95% to 14.19%, and the neutral detergent fiber (NDF) and acid detergent fiber (ADF) contents ranged from 45.53% to 70.88% and 34.17% to 49.83%, respectively. Among the amaranth varieties, A. hypochondriacus 48 showed the most excellent ruminant feed nutrient quality (CP, 14.19%; NDF, 45.53%; and ADF, 34.17%). The effective degradabilities of dry matter (EDDM; 33–56%) and crude protein EDCP (27–59%) of the amaranth were lower compared to other studies, which could be due to the maturity stage at which the forages were harvested. Nonetheless, A. hypochondriacus 48 showed the highest EDDM (56.73%) and EDCP (59.09%). The different amaranth species did not differ greatly in terms of total VFA concentration or molar proportions, total gas production, or ammonia-N concentration. The high nutrient composition, and highly effective degradability of dry matter and crude protein, coupled with the favorable fermentation characteristics, suggest that the amaranth forages showed good to excellent feed quality for cattle.

In situ degradability of organic matter, crude protein and cell wall of various tree forages

1992 ◽  
Vol 55 (1) ◽  
pp. 29-34 ◽  
Author(s):  
P. Kamatali ◽  
E. Teller ◽  
M. Vanbelle ◽  
G. Collignon ◽  
M. Foulon
Keyword(s):  
Cell Wall ◽  
Organic Matter ◽  
Leucaena Leucocephala ◽  
Crude Protein ◽  
In Vitro Digestibility ◽  
Sesbania Sesban ◽  
Holstein Friesian ◽  
Ruminal Degradation

AbstractLeaves of Leucaena leucocephala, Sesbania sesban and Calliandra callothyrsus were harvested in Rwanda from young shoots at 8 weeks after the first cutting. They were dried, ground and incubated at the same time in polyester bags in three non-lactating Holstein-Friesian cows each fitted with a ruminal cannula. The bags were removed at 0, 2, 4, 8, 24, 48, 72 and 144 h after the start of incubations. The different parameters characterizing extent and rate of ruminal degradation of organic matter (OM), neutral-detergent fibre (NDF), and crude protein (CP) were calculated. In vitro digestibility of residual protein after 24 h and 48 h incubation was also determined. Sesbania sesban had lowest cell wall contents and gave highest ruminal degradability for OM, NDF and CP. Leucaena leucocephala was degraded to a lesser extent, but its undegraded protein had a somewhat higher in vitro digestibility. In contrast, protein of Calliandra callothyrsus was poorly degraded and digested. The proportion and composition of cell wall could not explain these differences in digestion characteristics and other measurements, such as tannins, were incriminated. Increased ruminal incubation time augmented the extent of ruminal degradation and reduced in vitro digestibility of undegraded protein but did not affect the undigestible protein fraction.

scholarly journals Degradabilidad in situ de la materia seca, proteína y fibra del forraje de morera (Morus alba).

2006 ◽  
Vol 12 (1) ◽  
pp. 79
Author(s):  
Carlos Boschini
Keyword(s):  
Crude Protein ◽  
Dry Matter ◽  
Soluble Fraction ◽  
Neutral Detergent Fiber ◽  
Whole Plant ◽  
Degradation Rates ◽  
Entire Plant ◽  
Ruminal Degradation ◽  
Ruminal Degradability

The purpose of this study was to determine the ruminal degradability of dry matter, crude protein, neutral detergent fiber and their fractions, contained in regrowth of mulberry at 56, 84 and 112 days. The degradation of leaves, stems and the whole plant was calculated. The potential degradability of these three components diminished as the regrowth aged. The soluble fraction of the dry matter was reduced from 29% to 25% in the leaves and from 27% to 19% in the stems between the 56th and the112th day. The degradable fraction in the stem remained constant (61-63%) on the three measuring days, with ruminal degradation levels of 14%, 10% and 9%/h respectively. In the stem the degradable fraction diminished from 42% to 30% between the 56th and 112th day, with degradation speeds of 8.4%, 6.7 % and 5.3%/h. The soluble crude protein diminished with age from 41% to 33% in the entire plant and the degradable fraction remained constant between 49% and 51%. The degradation rate was higher than 9.4%/h. The neutral detergent fiber had a degradable fraction of 71%, 62% y 43% on the 56th, 84th and 112th day, with degradation rates of 13%, 10% y 9%/h respectively. The degradation of the neutral detergent fiber began in the rumen with a lag time of 1.5 to 3.2 hours.

scholarly journals Ruminal Degradability and Summative Models Evaluation for Total Digestible Nutrients Prediction of Some Forages and Byproducts in Goats

2013 ◽  
Vol 2013 ◽  
pp. 1-8 ◽  
Author(s):  
Oswaldo Rosendo ◽  
Luis Freitez ◽  
Rafael López
Keyword(s):  
Chemical Composition ◽  
Crude Protein ◽  
Dry Matter ◽  
National Research Council ◽  
Neutral Detergent Fiber ◽  
Coefficient Of Determination ◽  
Ruminal Degradability ◽  
Lineal Regression

In in vitro true dry matter degradability (IVTDMD), in situ dry matter degradability, and neutral detergent fiber degradability, both in vitro (IVNDFD) and in situ (ISNDFD) techniques were used with crossbred goats to determine dry matter and neutral detergent fiber (NDF) ruminal degradability in eight forages and four industrial byproducts. Total digestible nutrients (TDN) content obtained with five different summative models (summative equations) were studied to compare the precision of estimates. All these models included digestible fractions of crude protein, ether extract, and nonfiber carbohydrates that were calculated from chemical composition, but digestible NDF (dNDF) was obtained from IVNDFD (IVdNDF), ISNDFD (ISdNDF), or by using the Surface Law approach. On the basis of the coefficient of determination (R2) of the simple lineal regression of predicted TDN (y-axes) and observed IVTDMD (x-axes), the precision of models was tested. The predicted TDN by the National Research Council model exclusively based on chemical composition only explains up to 41% of observed IVTDMD values, whereas the model based on IVdNDF had a high precision (96%) to predict TDN from forage and byproducts fiber when used in goats.

scholarly journals Ruminal degradation of elephant grass silages added with faveira pods

2018 ◽  
Vol 40 (1) ◽  
pp. 39946 ◽  
Author(s):  
Bruno Spindola Garcez ◽  
Claudiane Morais dos Santos ◽  
Cicero Nicolini ◽  
Francisco Araújo Machado ◽  
Ernando De Oliveira Macedo ◽  
...  
Keyword(s):  
Crude Protein ◽  
Dry Matter ◽  
Time Lag ◽  
Neutral Detergent Fiber ◽  
Elephant Grass ◽  
Ruminal Degradation ◽  
Ruminal Degradability ◽  
Colonization Time ◽  
Matter Basis

 The objective of this study was to evaluate in situ ruminal degradability of elephant grass silages with addition of 8, 16 and 24% of faveira pods in experimental silos of 100x50 mm, equipped with bunsen valves. The content of crude protein (cp) and neutral detergent fiber (ndf) were determined on a dry matter basis. To evaluate the in situ degradability of dm, cp and ndf, nylon bags containing 4 g sample were incubated in the rumen of three fistulated cattle for 6, 24 and 72h. The dm and cp content of elephant grass silages increased (p < 0.05) from 8% inclusion associated with a higher concentration of constituents in faveira pods (77.25 and 9.61% dm). The ndf fraction reduced 10.91% (p < 0.05) when adding 24% pods. The potential degradation (pd) of dm and cp increased with inclusion of faveira pods, with 75.97 and 95.21%, respectively, for the level of 24%. There was increased potentially degradable fraction (bp) of ndf by 7.07% with inclusion of 24% faveira pods, as well as a reduced colonization time (lag) from 3.81 to 3.44 hours. The addition of up to 24% faveira pods to elephant grass silages improves rumen microbial degradation, and it is indicated this level of addition to obtain better quality silage. 

scholarly journals Potential of Recycling Cauliflower and Romanesco Wastes in Ruminant Feeding: In Vitro Studies

Animals ◽  
2020 ◽  
Vol 10 (8) ◽  
pp. 1247 ◽  
Author(s):  
Trinidad de Evan ◽  
Andrea Vintimilla ◽  
Eduarda Molina-Alcaide ◽  
María Ranilla ◽  
María Carro
Keyword(s):  
Crude Protein ◽  
Dry Matter ◽  
Energy Content ◽  
Metabolizable Energy ◽  
Neutral Detergent Fiber ◽  
Ruminal Fermentation ◽  
Ch4 Production ◽  
Intestinal Digestibility

The nutritive values for ruminants of cauliflower (CAU) and Romanesco (ROM) wastes (leaves, stems and sprouts) were assessed by analyzing their chemical composition, in vitro ruminal fermentation, and in vitro intestinal digestibility. In addition, the in vitro ruminal fermentation of diets containing increasing amounts of CAU was studied. The dry matter (DM) content of leaves, stems and sprouts of both vegetables was lower than 10%, but they contained high crude protein (CP; 19.9 to 33.0%) and sugar (16.3 to 28.7%) levels, and low neutral detergent fiber (21.6 to 32.3%). Stems and sprouts were more rapidly and extensively fermented in the rumen than leaves, but there were only minor differences the fermentation profiles of both vegetables. The estimated metabolizable energy content ranged from 9.3 (leaves) to 10.8 (sprouts) MJ/kg DM. The CP rumen degradability (12-h in situ incubations) was greater than 80.0% for all fractions, and the in vitro intestinal digestibility of CP ranged from 85.7 to 93.2%. The inclusion of up to 24% of dried CAU in the concentrate of a mixed diet (40:60 alfalfa hay:concentrate) increased the in vitro rumen fermentation of the CAU diet, but did not affect methane (CH4) production, indicating the lack of antimethanogenic compounds in CAU.

scholarly journals Tannin concentration and degradation rate in vitro of Morus alba and Hibiscus rosa-sinensis

2019 ◽  
Vol 29 ◽  
pp. 1-6
Author(s):  
Aldenamar Cruz Hernández ◽  
David Hernández Sanchez ◽  
Armando Gómez-Vázquez ◽  
Alejandra Govea-Luciano ◽  
Juan M. Pinos-Rodríguez ◽  
...  
Keyword(s):  
Condensed Tannins ◽  
Crude Protein ◽  
Degradation Rate ◽  
Condensed Tannin ◽  
Morus Alba ◽  
Neutral Detergent Fiber ◽  
In Vitro Degradation ◽  
Hibiscus Rosa Sinensis ◽  
Tannin Concentration

The study evaluated the influence of age (30, 60, 90 and 120 days) on dry matter (DM), crude protein (CP), neutral detergent fiber (NDF), free condensed tannins (FCT), fiber-bound condensed tannins (FBCT), protein-bound condensed tannin content (PBCT), in vitro degradation rate of DM (DMkd), CP (CPkd) and NDF (NDFkd) of Morus alba (MA) and Hibiscus rosa-sinensis (HRS). For MA leaves, there was a quadratic (p < 0.01) relationship between CP content and age: the CP content of 60, 90 and 120 days-old leaves was similar, but it was lower than that of 30 days-old leaves. For HRS leaves, CP content decreased linearly (p < 0.01) as age increased, and NDF content increased linearly (p < 0.01). For both MA and HRS, there were cubit (p < 0.01) effects of age on FCT, FBCT and PBCT content, in which the highest values were found in 120-d-old leaves and the lowest in 30 and 90 d-old leaves. Furthermore, old leaves had a linear decrease in DMkd, CPkd, and NDFkd. DMkd, CPkd, and NDFkd negatively correlated (p < 0.001) with FCT, FBCT, and PBCT content. For both MA and HRS, 90 days-old leaves had higher nutritional value than 120 days-old leaves.

scholarly journals In vitro and in situ techniques yield different estimates of ruminal disappearance of barley

2019 ◽  
Vol 4 (1) ◽  
pp. 141-148 ◽  
Author(s):  
Mary E DeFeo ◽  
Kelsey V Shampoe ◽  
Pedro H V Carvalho ◽  
Flavia A S Silva ◽  
Tara L Felix
Keyword(s):  
Repeated Measures ◽  
Neutral Detergent Fiber ◽  
Laboratory Methods ◽  
Hulless Barley ◽  
In Vitro Techniques ◽  
In Situ Techniques ◽  
In Vitro Incubation ◽  
Ruminal Degradation

Abstract Objectives were to compare in vitro and in situ disappearance of dry matter (DM), neutral detergent fiber (NDF), and starch of traditional (unprocessed and rolled) and hulless (unprocessed) barley. Experiment 1: three barley sources were compared using in vitro techniques. The sources were: 1) traditional barley that was not processed, 2) traditional barley processed through a roller mill, and 3) hulless barley that was not processed. For in vitro incubation, each barley source was ground through a 1-mm screen. Ground barley sources were weighed into bags (25 micron porosity) and incubated in ruminal fluid from two steers fed 80% rolled corn for 3, 6, 12, 24, 48, or 72 h. Intact bags were assayed for NDF; remaining bags were opened and the residual was removed and analyzed to determine disappearance of DM and starch. Experiment 2: the barley sources used in Exp. 1 were compared using in situ techniques. For in situ analysis, each barley source was ground in a Wiley mill with no screen to mimic mastication. Artificially masticated samples were weighed into Dacron bags (50 ± 10 micron porosity) and incubated in eight ruminally fistulated steers (n = 8) for 3, 6, 12, 24, 48, and 72 h. Residual contents were analyzed to determine in situ disappearance of DM, NDF, and starch. Data were analyzed using the MIXED procedures of SAS (9.4 SAS Institute, Cary, NC) with repeated measures. DM disappearance was greatest (P &lt; 0.05) for hulless barley in vitro and for rolled barley in situ, regardless of time postincubation. For both trials, NDF disappearance was greatest (P &lt; 0.05) for hulless barley, regardless of time postincubation. Starch disappearance at all time points was greatest (P &lt; 0.05) for rolled barley in situ. Starch disappearance was greater (P &lt; 0.05) for hulless barley at 6 h of in vitro incubation compared to rolled and unprocessed barley, whereas starch disappearance in vitro was comparable (P = 0.60) between barley sources. When the grains were compared in vitro, minor differences were noted, presumably because barley sources were finely ground prior to incubation. Compared to in vitro estimates, in situ techniques had greater variation in ruminal degradation estimates. Differences observed between in situ and in vitro techniques are driven largely by differences between the procedures. Although laboratory methods are widely used to estimate ruminal degradation, these techniques did not provide comparable estimates of ruminal degradation of barley.

scholarly journals Evaluation of the Nutritive Value and the Fatty Acid, Phenol, Tannin and Terpenoid Contents of Nine Pastures in an Alpine District during the Summer Season

Agriculture ◽  
2020 ◽  
Vol 10 (2) ◽  
pp. 42
Author(s):  
Pier Giorgio Peiretti ◽  
Sonia Tassone ◽  
Narges Vahdani ◽  
Giovanna Battelli ◽  
Francesco Gai
Keyword(s):  
Crude Protein ◽  
Nutritive Value ◽  
Condensed Tannin ◽  
Neutral Detergent Fiber ◽  
Total Phenols ◽  
True Digestibility ◽  
Balanced Distribution ◽  
Gross Energy ◽  
Nw Italy

A study was conducted over the summer of 2014 on nine Alpine pastures in the Chisone and Susa Valleys (NW Italy). The aim was to characterize the variation in the chemical composition, gross energy, in vitro true digestibility (IVTD), in vitro neutral detergent fiber digestibility (NDFD), fatty acids (FA), total phenols, total and condensed tannin contents, and terpenoid profile. The dry matter, ash, crude protein, acid detergent fiber, lignin, and gross energy contents of the pastures were found to differ. All the pastures had good IVTD (706–829 g/kg DM) and NDFD (487–694 g/kg NDF) values. The most abundant FAs in all the pastures were α-linolenic (354–519 g/kg of the total FAs), linoleic (75–110 g/kg of the total FAs), and palmitic acid (64–89 g/kg of the total FAs) and they differed significantly among pastures. No significant differences were found in the total phenols, or in the total and condensed tannin contents among pastures. Fifty-eight terpenoids were detected and 4-cyclopentene-1, 3-dione, β-caryophyllene, and eucalyptol were the most abundant. The terpenoids differed both qualitatively and quantitatively among pastures. The results highlight the importance of the great biodiversity of pastures, which provide a balanced distribution of fundamental nutritional elements and bioactive compounds in grasslands.

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