scholarly journals Peptide dendrimers: drug/gene delivery and other approaches

2017 ◽  
Vol 95 (9) ◽  
pp. 907-916 ◽  
Author(s):  
S.S. Santos ◽  
R.V. Gonzaga ◽  
J.V. Silva ◽  
D.F. Savino ◽  
D. Prieto ◽  
...  
Keyword(s):  
Gene Delivery ◽  
Bioactive Compounds ◽  
Recent Trend ◽  
Bioactive Compound ◽  
Delivery Systems ◽  
Bioactive Molecules ◽  
Peptide Dendrimers ◽  
Important Trend ◽  
Covalently Linked ◽  
Peptide Dendrimer

Dendrimers are versatile hyperbranched molecules, which have deserved attention especially for their potential in many applications, including biological. Peptide dendrimers comprise interesting classes of dendrimers, and their use has been emphasized as a drug/bioactive compound delivery system, mostly in the antineoplastic area. The bioactive molecules can be covalently linked or entrapped inside the peptide derivative. Self-assembled nanocarriers are a recent trend in the design of potential delivery systems, and pH-sensitive carriers, one of their methods, have been designed to control their systems. In addition, the use of targeting peptides or other specific groups that direct the drug/bioactive compounds to specific organs is an important trend in the search for better drug delivery systems. Recent examples have been given in the literature, showing that gene delivery as another important peptide dendrimer application. It is worth emphasizing that some peptide dendrimers show activity per se, without bioactive compounds. Immune compounds and vaccines are presented herein, as well as uses of other peptide dendrimers are briefly discussed in this review, which encompasses around 10 years of work.

scholarly journals Plant Protein-Based Delivery Systems: An Emerging Approach for Increasing the Efficacy of Lipophilic Bioactive Compounds

Molecules ◽  
2021 ◽  
Vol 27 (1) ◽  
pp. 60
Author(s):  
Andresa Gomes ◽  
Paulo José do Amaral Sobral
Keyword(s):  
Bioactive Compounds ◽  
Bioactive Compound ◽  
Delivery Systems ◽  
Raw Material ◽  
Plant Protein ◽  
Plant Proteins ◽  
Rational Development ◽  
Effective Delivery ◽  
New Generation ◽  
And Control

The development of plant protein-based delivery systems to protect and control lipophilic bioactive compound delivery (such as vitamins, polyphenols, carotenoids, polyunsaturated fatty acids) has increased interest in food, nutraceutical, and pharmaceutical fields. The quite significant ascension of plant proteins from legumes, oil/edible seeds, nuts, tuber, and cereals is motivated by their eco-friendly, sustainable, and healthy profile compared with other sources. However, many challenges need to be overcome before their widespread use as raw material for carriers. Thus, modification approaches have been used to improve their techno-functionality and address their limitations, aiming to produce a new generation of plant-based carriers (hydrogels, emulsions, self-assembled structures, films). This paper addresses the advantages and challenges of using plant proteins and the effects of modification methods on their nutritional quality, bioactivity, and techno-functionalities. Furthermore, we review the recent progress in designing plant protein-based delivery systems, their main applications as carriers for lipophilic bioactive compounds, and the contribution of protein-bioactive compound interactions to the dynamics and structure of delivery systems. Expressive advances have been made in the plant protein area; however, new extraction/purification technologies and protein sources need to be found Their functional properties must also be deeply studied for the rational development of effective delivery platforms.

Therapeutic Efficacy of Herbal Formulations Through Novel Drug Delivery Systems

2021 ◽  
pp. 1-42
Author(s):  
Arvind Kumar Singh Chandel ◽  
Nutan Bhingradiya
Keyword(s):  
Drug Delivery ◽  
Drug Delivery Systems ◽  
Herbal Medicines ◽  
Bioactive Compound ◽  
Delivery Systems ◽  
Bioactive Molecules ◽  
Therapeutic Molecules ◽  
Dose Accuracy ◽  
The Many ◽  
Novel Drug

The herbal-based drugs have been getting wide attention over the last few decades. The herbal drugs have been used from the ancient times, but the dose accuracy, efficacy, and treatment was just based on symptomatic observatory. Modern research is focused on pure bioactive compound instead of crude. The researchers are trying to apply the current advanced nanotechnology-based knowledge on the herbal medicines to improve efficacy and reduces the unwanted pharmacological actions' adverse effects of the therapeutic molecules. The many novel drug delivery systems available used for the herbal bioactive molecules include polymeric micelle, injectable hydrogel, nano gel, nano emulsions, microsphere, polymeric nanoparticles, nano capsules, liposomes, phytosomes, transferosomes, dendrimer, and ethosomes. This chapter highlights the existing research and development of current standing of the of various novel formulations especially for the herbal bioactive molecules and summarizes their active ingredients, commercial formulations, route of drug administration, and pharmacological activity.

scholarly journals Why the Orientational Mobility in Arginine and Lysine Spacers of Peptide Dendrimers Designed for Gene Delivery Is Different?

2020 ◽  
Vol 21 (24) ◽  
pp. 9749
Author(s):  
Valeriy V. Bezrodnyi ◽  
Oleg V. Shavykin ◽  
Sofia E. Mikhtaniuk ◽  
Igor M. Neelov ◽  
Nadezhda N. Sheveleva ◽  
...  
Keyword(s):  
Molecular Dynamics ◽  
Molecular Dynamics Simulation ◽  
Gene Delivery ◽  
Structural Properties ◽  
Dynamics Simulation ◽  
Contour Length ◽  
Pairing Effect ◽  
Slowing Down ◽  
Peptide Dendrimers ◽  
Peptide Dendrimer

New peptide dendrimer with Lys-2Arg repeating units was recently studied experimentally by NMR (RSC Advances, 2019, 9, 18018) and tested as gene carrier successfully (Int. J. Mol. Sci., 2020, 21, 3138). The unusual slowing down of the orientational mobility of 2Arg spacers in this dendrimer was revealed. It has been suggested that this unexpected behavior is caused by the Arg-Arg pairing effect in water, which leads to entanglements between dendrimer branches. In this paper, we determine the reason for this slowing down using atomistic molecular dynamics simulation of this dendrimer. We present that the structural properties of Lys-2Arg dendrimer are close to those of the Lys-2Lys dendrimer at all temperatures (Polymers, 2020, 12, 1657). However, the orientational mobility of the H-H vector in CH2-N groups of 2Arg spacers in Lys-2Arg dendrimer is significantly slower than the mobility of the same vector in the Lys-2Lys dendrimer. This result is in agreement with the recent NMR experiments for the same systems. We revealed that this difference is not due to the arginine-arginine pairing, but is due to the semiflexibility effect associated with the different contour length from CH2-N group to the end of the side arginine or lysine segment in spacers.

An NLS peptide covalently linked to linear DNA does not enhance transfection efficiency of cationic polymer based gene delivery systems

2005 ◽  
Vol 7 (2) ◽  
pp. 208-217 ◽  
Author(s):  
M. A. E. M. van der Aa ◽  
G. A. Koning ◽  
C. d'Oliveira ◽  
R. S. Oosting ◽  
K. J. Wilschut ◽  
...  
Keyword(s):  
Gene Delivery ◽  
Transfection Efficiency ◽  
Delivery Systems ◽  
Cationic Polymer ◽  
Linear Dna ◽  
Covalently Linked

Nutraceuticals from Marine Bionetworks

2019 ◽  
Vol 15 (4) ◽  
pp. 338-344
Author(s):  
Abhitav Tiwari ◽  
Shambhawi Pritam ◽  
Keerti Mishra ◽  
Mehshara Khan ◽  
Neeraj Upmanyu ◽  
...  
Keyword(s):  
Dietary Supplements ◽  
Bioactive Compounds ◽  
Daily Basis ◽  
Marine Organisms ◽  
Bioactive Molecules ◽  
Natural Sources ◽  
Nutraceutical Compounds ◽  
Potential Sources ◽  
Immense Potential ◽  
Potential Use

“Nutrition” and “Pharmaceutical” together build up the perception of “Nutraceuticals” that refer to the food or dietary supplements that help to incorporate additional health benefits to the fundamental sustenance accomplished on daily basis. Each nutraceutical contains one or more bioactive molecules that are usually obtained by chemical and/ or biotechnological synthesis or by extraction from natural sources. Among the natural sources, marine bionetwork possess immense potential for the presence of bioactive compounds. Some of these bioactive compounds as isolated from marine sources, have potential use as nutraceuticals. This mini review provides a brief overview of nutraceutical compounds from marine sources that are currently under research and/or have been commercialized. A detailed discussion on the biochemical categories of compounds and the marine organisms that play as potential sources of these bioactive nutraceutical compounds have been included.

Star Shaped Poly(ethylene glycols) Yield Biocompatible Gene Delivery Systems

2015 ◽  
Vol 2 (4) ◽  
pp. 182-195 ◽  
Author(s):  
Jayanant Iemsam-Arng ◽  
Xiao Kong ◽  
Andreas G. Schatzlein ◽  
Ijeoma F. Uchegbu
Keyword(s):  
Gene Delivery ◽  
Delivery Systems ◽  
Ethylene Glycols ◽  
Poly Ethylene

scholarly journals Highly Osmotic Oxidized Sucrose-Crosslinked Polyethylenimine for Gene Delivery Systems

Pharmaceutics ◽  
2021 ◽  
Vol 13 (1) ◽  
pp. 87
Author(s):  
Jaehong Park ◽  
Kyusik Kim ◽  
Sohee Jeong ◽  
Migyeom Lee ◽  
Tae-il Kim
Keyword(s):  
Gene Delivery ◽  
Reductive Amination ◽  
Transfection Efficiency ◽  
Delivery Systems ◽  
Buffering Capacity ◽  
Serum Stability ◽  
High Osmolality ◽  
Cox 2 ◽  
Serum Free Conditions ◽  
Positively Charged

In this work, highly osmotic oxidized sucrose-crosslinked polyethylenimine (SP2K) polymers were developed for gene delivery systems, and the transfection mechanism is examined. First, periodate-oxidized sucrose and polyethylenimine 2K (PEI2K) were crosslinked with various feed ratios via reductive amination. The synthesis was confirmed by 1H NMR and FTIR. The synthesized SP2K polymers could form positively charged (~40 mV zeta-potential) and nano-sized (150–200 nm) spherical polyplexes with plasmid DNA (pDNA). They showed lower cytotoxicity than PEI25K but concentration-dependent cytotoxicity. Among them, SP2K7 and SP2K10 showed higher transfection efficiency than PEI25K in both serum and serum-free conditions, revealing the good serum stability. It was found that SP2K polymers possessed high osmolality and endosome buffering capacity. The transfection experiments with cellular uptake inhibitors suggest that the transfection of SP2K polymers would progress by multiple pathways, including caveolae-mediated endocytosis. It was also thought that caveolae-mediated endocytosis of SP2K polyplexes would be facilitated through cyclooxygenase-2 (COX-2) expression induced by high osmotic pressure of SP2K polymers. Confocal microscopy results also supported that SP2K polyplexes would be internalized into cells via multiple pathways and escape endosomes efficiently via high osmolality and endosome buffering capacity. These results demonstrate the potential of SP2K polymers for gene delivery systems.

scholarly journals Current Strategies for Studying the Natural and Synthetic Bioactive Compounds in Food by Chromatographic Separation Techniques

Processes ◽  
2021 ◽  
Vol 9 (7) ◽  
pp. 1100
Author(s):  
Wioletta Parys ◽  
Małgorzata Dołowy ◽  
Alina Pyka-Pająk
Keyword(s):  
Gas Chromatography ◽  
Bioactive Compounds ◽  
Residue Analysis ◽  
Food Products ◽  
Vital Role ◽  
Bioactive Molecules ◽  
Validation Parameters ◽  
Layer Chromatography ◽  
Natural And Synthetic

The present study summarizes the new strategies including advanced equipment and validation parameters of liquid and gas chromatography methods i.e., thin-layer chromatography (TLC), column liquid chromatography (CLC), and gas chromatography (GC) suitable for the identification and quantitative determination of different natural and synthetic bioactive compounds present in food and food products, which play an important role in human health, within the period of 2019–2021 (January). Full characteristic of some of these procedures with their validation parameters is discussed in this work. The present review confirms the vital role of HPLC methodology in combination with different detection modes i.e., HPLC-UV, HPLC-DAD, HPLC-MS, and HPLC-MS/MS for the determination of natural and synthetic bioactive molecules for different purposes i.e., to characterize the chemical composition of food as well as in the multi-residue analysis of pesticides, NSAIDs, antibiotics, steroids, and others in food and food products.

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