Intensive Swimming Exercise-Induced Oxidative Stress and Reproductive Dysfunction in Male Wistar Rats: Protective Role of α-Tocopherol Succinate

2004 ◽  
Vol 29 (2) ◽  
pp. 172-185 ◽  
Author(s):  
Indranil Manna ◽  
Kuladip Jana ◽  
Prabhat Kumar Samanta
Keyword(s):  
Oxidative Stress ◽  
Body Weight ◽  
Hydroxysteroid Dehydrogenase ◽  
Protective Role ◽  
Male Rats ◽  
Swimming Exercise ◽  
Control Group ◽  
Male Wistar Rats ◽  
Exercise Induced ◽  
Pachytene Spermatocytes

In the present study, 30 male rats (age 3 mos, Wt 128.6 ± 3.7 g) were randomly divided into Control group (CG), Experimental group (EG), and Supplemented group (SG), 10 per group. An exercise protocol (3 hrs swimming per day, 5 days a week for 4 weeks) was followed in EG and SG, with no exercise in CG. In SG, α-tocopherol succinate was injected subcutaneouly at a dose of 50 mg•kg−1 per body weight per day. After 4 weeks of exercise, significant diminutions (p <  0.05) were noted in somatic indices of testes and accessory sex organs; seminiferous tubuler diameter (STD); testicular Δ5, 3β-hydroxysteroid dehydrogenase (Δ5, 3β-HSD) 17 β-hydroxysteroid dehydrogenase (17 β-HSD) activities; plasma levels of testosterone (T), luteinizing hormone (LH); preleptotine spermatocytes (pLSc), mid-pachytene spermatocytes (mPSc), and Stage 7 spermatids (7 Sd); testicular α-tocopherol and glutathione (GSH) content; superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPX), and glutathione-s-transferase (GST) activities in EG when compared to CG. Moreover, a significant elevation (p <  0.05) in malondialdehyde (MDA) was found in testes of EG compared to CG. No significant alteration was noted in body weight among the groups. Co-administration of α-tocopherol succinate restored the above parameters. Intensive swimming exercise-induced oxidative stress causes dysfunction in the male reproductive system, which can be protected by α-tocopherol succinate. Key words: spermatogenesis, steroidogenesis, lipid peroxidation, vitamin E

Protective Role of Polysaccharides from Gynostemma pentaphyllum Makino Supplementation against Exhaustive Swimming Exercise-Induced Oxidative Stress in Liver and Skeletal Muscle of Mice

2014 ◽  
Vol 962-965 ◽  
pp. 1231-1234
Author(s):  
Hui Huang ◽  
Bo Qi
Keyword(s):  
Oxidative Stress ◽  
Skeletal Muscle ◽  
Protective Role ◽  
Swimming Exercise ◽  
Control Group ◽  
High Dose ◽  
Gynostemma Pentaphyllum ◽  
Exercise Induced ◽  
Different Doses

The objective of this study was to investigate the protective role of polysaccharide fromGynostemma pentaphyllumMakino (PGP) supplementation against exhaustive swimming exercise-induced oxidative stress. A total of 48 mice were randomly divided into four groups: control, low-dose, medium-dose, and high-dose PGP supplementation groups. The control group received distilled water and the supplementation groups received different doses of PGP (50, 100 and 200 mg/kg body weight) by gavage once a day for 28 consecutive days. After 28 days, the mice performed an exhaustive swimming exercise, and some biochemical parameters related to oxidative stress, including superoxide dismutase (SOD), glutathione peroxidase (GPx), catalase (CAT) and malondialdehyde (MDA), were measured. The results showed that PGP supplementation could increase SOD, GPx and CAT contents, as well as decrease MDA contents in the liver and skeletal muscle of mice, which suggests that PGP supplementation has a protective role against exhaustive swimming exercise-induced oxidative stress.

Effect of Opuntia dejecta (Salm-Dyck) flowers in liver of fructose-fed rats: Biochemicals, enzymatic and histological studies

2021 ◽  
pp. 096032712110134
Author(s):  
O Zouaoui ◽  
K Adouni ◽  
A Jelled ◽  
A Thouri ◽  
A Ben Chrifa ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Activity ◽  
Body Weight ◽  
Diabetes Type 2 ◽  
Male Rats ◽  
Control Group ◽  
Standard Drug ◽  
High Fructose ◽  
Group A ◽  
Histological Architecture

Phytochemical composition and antioxidant activity of flowers decoction at post-flowering stage (F3D) of Opuntia dejecta were determined. The obtained findings demonstrate that F3D has a marked antioxidant activity in all tested assays. Furthermore, the present study was designed to test the protective activity of F3D against induced Diabetes type 2 (DT2) in male rats. Those metabolic syndromes were induced by a high-fructose diet (HFD) (10% fructose solution) for a period of 20 weeks. F3D was administered orally (100 and 300 mg/kg body weight) daily for the last 4 weeks. Metformin (150 mg/kg body weight) was used as a standard drug and administrated orally for the last 4 weeks. The results showed a significant increase in blood glucose, triglycerides and hepatic markers (ALAT, ASAT and ALK-P) in HFD group. A significant increase in hepatic TBARS and a significant decrease in SOD, CAT and GPX were observed in fructose fed rats compared to control group. Administration of F3D showed a protective effect in biochemical and oxidative stress parameters measured in this study. Also, oral administration of F3D restored the histological architecture of rat liver in comparison with rats fed HFD. In conclusion, F3D attenuated hepatic oxidative stress in fructose-fed rats.

scholarly journals Quercetin Attenuates Pancreatic and Renal D-Galactose-Induced Aging-Related Oxidative Alterations in Rats

2020 ◽  
Vol 21 (12) ◽  
pp. 4348 ◽  
Author(s):  
Ali H. El-Far ◽  
Mohamed A. Lebda ◽  
Ahmed E. Noreldin ◽  
Mustafa S. Atta ◽  
Yaser H. A. Elewa ◽  
...  
Keyword(s):  
Body Weight ◽  
Physiological Saline ◽  
Basal Diet ◽  
Protective Role ◽  
Control Group ◽  
Functional Markers ◽  
Dependent Manner ◽  
Physiological Saline Solution ◽  
Male Wistar Rats ◽  
Bcl2 Protein

Aging is an oxidative stress-associated process that progresses with age. Our aim is to delay or attenuate these oxidative alterations and to keep individuals healthy as they age using natural compounds supplementation. Therefore, we conducted the present study to investigate the protective potentials of quercetin against D-galactose (D-gal)-associated oxidative alterations that were induced experimentally in male Wistar rats. Forty-five rats were randomly allocated into five groups of nine rats each. The groups were a control group that was reared on a basal diet and injected subcutaneously with 120 mg D-gal dissolved in physiological saline solution (0.9% NaCl) per kg body weight daily and quercetin-treated groups that received the same basal diet and subcutaneous daily D-gal injections were supplemented orally with 25, 50, and 100 mg of quercetin per kg body weight for 42 days. Pancreatic and renal samples were subjected to histopathological, immunohistochemical, and relative mRNA expression assessments. Aging (p53, p21, IL-6, and IL-8), apoptotic (Bax, CASP-3, and caspase-3 protein), proliferative (Ki67 protein), antiapoptotic (Bcl2 and Bcl2 protein), inflammatory (NF-κB, IL-1β, and TNF-α), antioxidant (SOD1), and functional markers (GCLC and GCLM genes and insulin, glucagon, and podocin proteins) were determined to evaluate the oxidative alterations induced by D-gal and the protective role of quercetin. D-gal caused oxidative alterations of the pancreas and kidneys observed via upregulations of aging, apoptotic, and inflammatory markers and downregulated the antiapoptotic, proliferative, antioxidant, and functional markers. Quercetin potentially attenuated these aging-related oxidative alterations in a dose-dependent manner. Finally, we can conclude that quercetin supplementation is considered as a promising natural protective compound that could be used to delay the aging process and to maintain human health.

scholarly journals Protective Role of Ce Nanoparticles Against the Hepatotoxicity Induced by Exposure to Paraquat

2018 ◽  
Vol 6 (2) ◽  
pp. 37-43
Author(s):  
Hamid Heidary Dartoti ◽  
Farzin Firozian ◽  
Sara Soleimani Asl ◽  
Akram Ranjbar
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Activity ◽  
Protective Role ◽  
Male Rats ◽  
Hepatic Tissue ◽  
Control Group ◽  
Serum Samples ◽  
Tissue Samples ◽  
Total Antioxidant ◽  
Group 2

Objectives: The present study aimed to investigate the antioxidant activity of cerium oxide nanoparticles (CeNPs) against paraquat (PQ)-induced liver injury in rats. Methods: Thirty-two male rats were divided into four 8-member groups and treated intraperitoneally with PQ and/or CeNPs for 14 days. Group 1 received PQ (5 mg/kg/d), group 2 received CeNPs (15, 30, and 60 mg/kg/d), group 3 received a combination of PQ (5 mg/kg/d) and CeNPs (15, 30, and 60 mg/kg/d), and group 4 (control group) received saline solution. Serum samples along with liver tissue samples were collected from all the rats. Oxidative stress (OS) biomarkers including total antioxidant capacity, lipid peroxidation, total thiol groups, DNA damage, and nitric oxide levels were determined. Histological samples were also analyzed using hematoxylin and eosin staining slides. Results: Levels of oxidative stress and hepatic tissue damage were significantly higher in the PQ group compared to the control group. CeNPs at a dose of 15 mg/kg showed the antioxidant activity and compromised the PQ-induced damage. Conclusion: In the scenario tested in this study, CeNPs could reduce the levels of OS, as well as hepatic damage induced by PQ.

scholarly journals THE COMPARATIVE ROLE OF ASCORBATE AND CHELATORS IN REVERSING OXIDATIVE STRESS, HEPATIC AND RENAL DYSFUNCTION IN SUB-ACUTE LEAD POISONING

2016 ◽  
Vol 14 (1) ◽  
pp. 89-102
Author(s):  
B. O. ONUNKWOR ◽  
R. N. UGBAJA ◽  
D. A. OMONIYI ◽  
A. O. DOSUMU
Keyword(s):  
Oxidative Stress ◽  
Body Weight ◽  
Lead Poisoning ◽  
Catalase Activity ◽  
Lead Level ◽  
Blood Lead ◽  
Therapeutic Interventions ◽  
Control Group ◽  
Male Wistar Rats ◽  
Species Production

Lead has been implicated in the induction of reactive species production, leading to organ dysfunctions. The ameliorative roles of ascorbate and chelators in acute lead poisoning were comparatively studied in thirty-five male Wistar rats (150-200g), segregated into 5 groups (n=7/Group): group 1(administered normal saline),ª¤? groups 2-5 were orally exposed to 75mg/kg body weight lead acetate (PbAc) daily for 14 days. Pre-therapy blood samples were collected to ascertain blood lead level (BLL) and catalase activity 24hours after the last PbAc exposure. Groups 3, 4, and 5 were then treated with 30mg/kg body weight D-penicillamine; 30mg/kg body weight succimer; and 500mg/kg body weight ascorbate respectively for 10 days, followed by the assay for indices of oxidative stress, hepatic and renal dysfunctions.ª¤? Results obtained showed significantly elevated BLL in the four groups exposed to PbAc. which were significantly reversed about 2 folds in groups 3-5 after therapeutic interventions. Pre-therapy blood catalase activity of the PbAc treated groups was significantly (p<0.05) reduced by 39% when compared with the control group, however ascorbate significantly (p<0.05) increased catalase activity by 2 folds above the control; decreased plasma activities of alanine transaminase and aspartate transaminase, blood urea nitrogen and creatinine among the groups administered therapeutics. These findings indicate that ascorbate is more effectiveª¤?

Polysaccharides from Ganoderma lucidum Supplementation Prevents Exhaustive Exercise-Induced Apoptosis in Mice Liver

2013 ◽  
Vol 864-867 ◽  
pp. 26-29
Author(s):  
Rong Yu ◽  
Shan Ma ◽  
Hao Wu
Keyword(s):  
Body Weight ◽  
Ganoderma Lucidum ◽  
Exhaustive Exercise ◽  
Swimming Exercise ◽  
Control Group ◽  
Exercise Induced ◽  
Mice Liver ◽  
Induced Apoptosis ◽  
Liver Tissues

The main purpose of this study was to examine the effects of polysaccharides from Ganoderma lucidum (GLP) supplementation on exhaustive exercise-induced apoptosis in mice liver. The mice were randomly divided into four groups of ten per group. One of the groups was the control group, the others were GLP treated groups (25, 50 and 100 mg/kg body weight). Exhaustive swimming exercise of mice were carried out after 28 days of GLP administration, and the expression of Bcl-2 and Bax proteins in the liver tissues were determined. The data showed that GLP supplementation was able to up-regulate the expression of Bcl-2 proteins and Bcl-2/Bax ratio, down-regulate the expression of Bax proteins. The results suggest that GLP supplementation prevents exhaustive exercise-induced apoptosis.

scholarly journals Chemopreventive Potential of Synergy1 and Soybean in Reducing Azoxymethane-Induced Aberrant Crypt Foci in Fisher 344 Male Rats

2011 ◽  
Vol 2011 ◽  
pp. 1-8 ◽  
Author(s):  
V. P. Gourineni ◽  
M. Verghese ◽  
J. Boateng ◽  
L. Shackelford ◽  
K. N Bhat
Keyword(s):  
Body Weight ◽  
Colon Carcinogenesis ◽  
Food Product ◽  
Protective Role ◽  
Aberrant Crypt Foci ◽  
Male Rats ◽  
Control Group ◽  
Hepatic Enzyme ◽  
Treatment Groups ◽  
Food Product Development

Synergy1, a prebiotic composed of Inulin and Oligofructose (1 : 1). Soybean meal is a natural source of isoflavones. The objective was to investigate the effects of feeding Synergy1 and SM on the incidence of azoxymethane- (AOM-) induced aberrant crypt foci (ACF) in Fisher 344 male rats. Rats (54) were randomly assigned to 9 groups (n=6). Control group (C) was fed AIN-93G and treatment groups Syn1 and SM at 5% and 10% singly and in combinations. Rats were injected with two s/c injections of AOM at 7 and 8 weeks of age at 16 mg/kg body weight and killed at 17 weeks by CO2asphyxiation. Colonic ACF enumeration and hepatic enzyme activities were measured. Reductions (%) in total ACF among treatment groups fed combinations were higher (67–77) compared to groups fed singly (52–64). Synergistic mechanisms among phytochemicals may be responsible suggesting protective role in colon carcinogenesis with implications in food product development.

scholarly journals Effects of Low-Frequency Electromagnetic Field on Oxidative Stress in Selected Structures of the Central Nervous System

2018 ◽  
Vol 2018 ◽  
pp. 1-8 ◽  
Author(s):  
Jan Budziosz ◽  
Agata Stanek ◽  
Aleksander Sieroń ◽  
Joanna Witkoś ◽  
Armand Cholewka ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Central Nervous System ◽  
Nervous System ◽  
Electromagnetic Field ◽  
Frontal Cortex ◽  
Low Frequency ◽  
Brain Structures ◽  
Male Rats ◽  
Control Group ◽  
Male Wistar Rats

Objective.The aim of the study was to evaluate the effects of a 28-day exposure to a 50 Hz electromagnetic field of 10 kV/m on the oxidative stress in selected rat central nervous system (CNS) structures. Material and Methods. Twenty male Wistar rats served as experimental subjects. Ten rats were exposed to an electromagnetic field with a frequency of 50 Hz, intensity of 10 kV/m, and magnetic induction of 4.3 pT for 22 hours a day. The control group of ten rats was subject to sham exposure. Homogenates of the frontal cortex, hippocampus, brainstem, hypothalamus, striatum, and cerebellum were evaluated for selected parameters of oxidative stress. Results. Following the four-week exposure to a low-frequency electromagnetic field, the mean malondialdehyde levels and total oxidant status of CNS structures did not differ significantly between the experimental and control groups. However, the activities of antioxidant enzymes in brain structure homogenates were decreased except for frontal cortex catalase, glutathione peroxidase, and hippocampal glutathione reductase. The low-frequency electromagnetic field had no effect on the nonenzymatic antioxidant system of the examined brain structures except for the frontal cortex. Conclusion. The four-week exposure of male rats to a low-frequency electromagnetic field did not affect oxidative stress in the investigated brain structures.

scholarly journals Protective role of ginseng extract against oxidative stress, reproductive and some biochemical parameters alterations induced by doxorubicin in male rats

2020 ◽  
Vol 8 (1) ◽  
pp. 78
Author(s):  
Mohammed Kassem ◽  
Abdel-Fattah Ali ◽  
Seham Y. Abo-kora ◽  
Nesreen Shawky
Keyword(s):  
Oxidative Stress ◽  
Biochemical Parameters ◽  
Semen Analysis ◽  
Treated Group ◽  
Protective Role ◽  
Male Rats ◽  
Control Group ◽  
Negative Effects ◽  
Ginseng Extract

This study investigates the modulating effect of ginseng against testicular toxicity, oxidative stress and changes in some biochemical parameters induced by doxorubicin. Twenty male rats were divided into four groups. The 1st group received distilled water orally (control group), The 2nd group received doxorubicin (5 mg/kg b.wt. intrapertenoineal) once a week for eight weeks, The 3rd group received ginseng extract (200 mg/kg b.wt.) daily for eight weeks and the 4th group received doxorubicin with ginseng extract by the same doses as in the 2nd and the 3rd groups respectively. At the end of the 8th week, blood and semen samples were taken for biochemical and semen analysis, respectively. The doxorubicin treated group had significantly higher serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), Creatine kinase (CK) and lactate dehydrogenase (LDH) along with lower levels of total protein, albumin and globulin. In addition, a significant decrease in antioxidant enzymes (SOD, CAT, GSHPx), and glutathione (GSH) associated with higher level of malondialdehyde (MDA) were observed. At the same time, the group that took doxorubicin with ginseng did not differ from control group in terms of these parameters. Male fertility study showed changes in testosterone and semen analysis in both groups treated with doxorubicin, while the group that took doxorubicin with ginseng showed an improvement towards control levels of these parameters. Thus ginseng supplement can reduce the negative effects of doxorubicin- induce.  

scholarly journals Whey Protein Concentrate WPC-80 Intensifies Glycoconjugate Catabolism and Induces Oxidative Stress in the Liver of Rats

Nutrients ◽  
2018 ◽  
Vol 10 (9) ◽  
pp. 1178 ◽  
Author(s):  
Marta Żebrowska-Gamdzyk ◽  
Mateusz Maciejczyk ◽  
Anna Zalewska ◽  
Katarzyna Guzińska-Ustymowicz ◽  
Anna Tokajuk ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Body Weight ◽  
Whey Protein ◽  
Transforming Growth Factor ◽  
Transforming Growth Factor Β1 ◽  
Whey Protein Concentrate ◽  
Control Group ◽  
Protein Concentrate ◽  
Male Wistar Rats ◽  
Tgf Β1

The aim of this study was to evaluate the effect of whey protein concentrate (WPC-80) on glycoconjugate catabolism, selected markers of oxidative stress and liver inflammation. The experiment was conducted on male Wistar rats (n = 63). The animals from the study group were administered WPC-80 at a dose of 0.3 or 0.5 g/kg body weight for 7, 14 or 21 days, while rats from the control group received only 0.9% NaCl. In liver homogenates, we assayed the activity of N-acetyl-β-D-hexosaminidase (HEX), β-glucuronidase (GLU), β-galactosidase (GAL), α-mannosidase (MAN), α-fucosidase (FUC), as well as the level of reduced glutathione (GSH), malondialdehyde (MDA), interleukin-1β (IL-1β) and transforming growth factor-β1 (TGF-β1). A significantly higher activity of HEX, GLU, MAN and FUC were found in the livers of rats receiving WPC-80 compared to controls. Serum ALT and AST were significantly higher in the animals supplemented with WPC-80 at a dose of 0.5 g/kg body weight for 21 days. In the same group of animals, enhanced level of GSH, MDA, IL-1β and TGF-β1 were also observed. WPC-80 is responsible for intensive remodelling of liver tissue and induction of oxidative stress especially at a dose of 0.5 g/kg body weight.

Sign in / Sign up

Export Citation Format

Share Document