Granule contents from rat polymorphonuclear leukocytes: antimicrobial properties and characterization

1986 ◽  
Vol 32 (6) ◽  
pp. 498-504 ◽  
Author(s):  
Richard L. Hodinka ◽  
Malcolm C. Modrzakowski
Keyword(s):  
Antibacterial Properties ◽  
Antimicrobial Properties ◽  
Bacterial Cells ◽  
Gram Positive Bacteria ◽  
Properties And Characterization ◽  
Bacterial Mutants ◽  
Killing Action ◽  
Gram Negative Organisms ◽  
Ph Range ◽  
Static Conditions

The nonoxidative antibacterial properties of isolated rat polymorphonuclear leukocyte granule contents were examined using Salmonella typhimurium LT-2 and a series of progressively rough lipopolysaccharide mutants of this strain as target bacteria. The granule extract was most active at 37 °C, with a substantial decrease in activity observed at lower temperatures. Deep rough bacterial mutants were killed best within a pH range of 6–8, while killing of mutants with increased lipopolysaccharide content was most efficient at an acid pH of 5. The activity of the extract was dependent on incubation time but was independent of the number of bacterial cells present in the assay mixture. The killing action of the granule extract was inhibited by the cations Na+, K+, Mg2+, Ca, and Fe2+. The degree of inhibition was dependent on the type and concentration of ion used. Rough mutants grown with aeration to log phase were killed more efficiently than the same mutants grown to stationary phase under static conditions. Also, gram-positive bacteria were more susceptible to the extract than were gram-negative organisms.

scholarly journals Purification of Bioactive Peptide with Antimicrobial Properties Produced by Saccharomyces cerevisiae

Foods ◽  
2020 ◽  
Vol 9 (3) ◽  
pp. 324 ◽  
Author(s):  
Shayma Thyab Gddoa Al-sahlany ◽  
Ammar Altemimi ◽  
Alaa Al-Manhel ◽  
Alaa Niamah ◽  
Naoufal Lakhssassi ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Antimicrobial Properties ◽  
Antibacterial Peptide ◽  
Inhibition Activity ◽  
Bacterial Strains ◽  
Gram Positive ◽  
Gram Negative ◽  
Gram Positive Bacteria ◽  
Viable Cells ◽  
Ph Range

A variety of organisms produce bioactive peptides that express inhibition activity against other organisms. Saccharomyces cerevisiae is considered the best example of a unicellular organism that is useful for studying peptide production. In this study, an antibacterial peptide was produced and isolated from Saccharomyces cerevisiae (Baker’s yeast) by an ultrafiltration process (two membranes with cut-offs of 2 and 10 kDa) and purified using the ÄKTA Pure 25 system. Antibacterial peptide activity was characterized and examined against four bacterial strains including Gram-positive and Gram-negative bacteria. The optimum condition for yeast growth and antibacterial peptide production against both Escherichia. coli and Klebsiella aerogenes was 25–30 °C within a 48 h period. The isolated peptide had a molecular weight of 9770 Da, was thermostable at 50–90 °C for 30 min, and tolerated a pH range of 5–7 at 4 °C and 25 °C during the first 24 h, making this isolated antibacterial peptides suitable for use in sterilization and thermal processes, which are very important aspect in food production. The isolated antibacterial peptide caused a rapid and steady decline in the number of viable cells from 2 to 2.3 log units of gram-negative strains and from 1.5 to 1.8 log units of gram-positive strains during 24 h of incubation. The isolated antibacterial peptide from Saccharomyces cerevisiae may present a potential biopreservative compound in the food industry exhibiting inhibition activity against gram-negative and gram-positive bacteria.

scholarly journals Antibacterial Composite Layers on Ti: Role of ZnO Nanoparticles

2016 ◽  
Vol 61 (1) ◽  
pp. 213-216
Author(s):  
A. Roguska ◽  
A. Belcarz ◽  
P. Suchecki ◽  
M. Andrzejczuk ◽  
M. Lewandowska
Keyword(s):  
Zno Nanoparticles ◽  
Antibacterial Properties ◽  
Antimicrobial Properties ◽  
Antimicrobial Activities ◽  
Bacterial Cells ◽  
Metallic Oxide ◽  
Moderate Amount ◽  
Orthopaedic Infections ◽  
Composite Layers ◽  
Biomedical Coatings

Problem of Post-operative infections of implant materials caused by bacterial adhesion to their surfaces is very serious. Enhancement of antibacterial properties is potentially beneficial for biomaterials value. Therefore, the metallic and metallic oxide nanoparticles attract particular attention as antimicrobial factors. The aim of this work was to create nanotubular (NT) oxide layers on Ti with the addition of ZnO nanoparticles, designed for antibacterial biomedical coatings. Antimicrobial activities of titanium, TiO2NT and ZnO/TiO2NT surfaces were evaluated against bacterial strain typical for orthopaedic infections: S. epidermidis. TiO2NT alone killed the free bacterial cells significantly but promoted their adhesion to the surfaces. The presence of moderate amount of ZnO nanoparticles significantly reduced the S. epidermidis cells adhesion and viability of bacterial cells in contact with modified surfaces. However, higher amount of loaded nanoZnO showed the reduced antimicrobial properties than the medium amount, suggesting the overdose effect.

scholarly journals Antibacterial Composite Layers on Ti: Role of ZnO Nanoparticles

2016 ◽  
Vol 61 (2) ◽  
pp. 937-940 ◽  
Author(s):  
A. Roguska ◽  
A. Belcarz ◽  
P. Suchecki ◽  
M. Andrzejczuk ◽  
M. Lewandowska
Keyword(s):  
Zno Nanoparticles ◽  
Antibacterial Properties ◽  
Antimicrobial Properties ◽  
Antimicrobial Activities ◽  
Bacterial Cells ◽  
Metallic Oxide ◽  
Moderate Amount ◽  
Orthopaedic Infections ◽  
Composite Layers ◽  
Biomedical Coatings

Abstract Problem of post-operative infections of implant materials caused by bacterial adhesion to their surfaces is very serious. Enhancement of antibacterial properties is potentially beneficial for biomaterials value. Therefore, the metallic and metallic oxide nanoparticles attract particular attention as antimicrobial factors. The aim of this work was to create nanotubular (NT) oxide layers on Ti with the addition of ZnO nanoparticles, designed for antibacterial biomedical coatings. Antimicrobial activities of titanium, TiO2 NT and ZnO/TiO2 NT surfaces were evaluated against bacterial strain typical for orthopaedic infections: S. epidermidis. TiO2 NT alone killed the free bacterial cells significantly but promoted their adhesion to the surfaces. The presence of moderate amount of ZnO nanoparticles significantly reduced the S. epidermidis cells adhesion and viability of bacterial cells in contact with modified surfaces. However, higher amount of loaded nanoZnO showed the reduced antimicrobial properties than the medium amount, suggesting the overdose effect.

The antimicrobial properties of new synthetic porphyrins

2003 ◽  
Vol 07 (11) ◽  
pp. 755-760 ◽  
Author(s):  
Tatyana O. Philippova ◽  
Boris N. Galkin ◽  
Oksana Yu. Zinchenko ◽  
Maria Yu. Rusakova ◽  
Vladimir A. Ivanitsa ◽  
...  
Keyword(s):  
Candida Albicans ◽  
Antimicrobial Properties ◽  
Middle Level ◽  
Membrane Receptors ◽  
Gram Negative Bacteria ◽  
Bacterial Cells ◽  
Free Base ◽  
Gram Positive ◽  
Gram Negative ◽  
Gram Positive Bacteria

The antimicrobial activity of new meso-tetrakis(N-methyl-6-quinolinyl)-substituted porphyrins and meso-tetrakis(N-methyl-6-quinolinyl)-substituted chlorins is described. The dark toxicity and photosensitising potentials of free-base (TQP and TQC) and its Sn(IV)-complexes [(TQP)Sn(IV) and (TQC)Sn(IV)] were tested on Gram-positive (Staphylococus aureus), Gram-negative (Escherichia coli and Pseudomonas aeruginosa) bacteria and two species of yeasts (Candida albicans and Rhodotorula bogoriensis). The results described in this paper show that TQP and (TQP)Sn(IV) did not inhibit the growth of S. aureus in the dark, but efficiently photosensitize the inactivation of this Gram-positive bacteria. These porphyrins have no appreciable photosensitizing activity towards Gram-negative bacteria. However, (TQP)Sn(IV) shows high dark toxicity against E. coli and P. aeruginosa. The free-base derivatives demonstrated dark activity only in the case of P. aeruginosa. We suppose that these meso-tetrakis(N-methyl-6-quinolinyl)-substituted porphyrins can bind to the Gram-negative bacteria outer membrane receptors that transported vitamin B12. The meso-substituted chlorins TQC and (TQC)Sn(IV) have shown similar efficiency in the dark- and photoinactivation of S. aureus. They revealed a middle level of dark toxicity towards Gram-negative bacteria. The Sn(IV)-complex of chlorin in comparison with free base and metalloporphyrins are more effective in photoinactivation of Gram-negative bacteria. Yeasts, such as Candida albicans and Rhodotorula bogoriensis are more sensitive to photodynamic inactivation as bacterial cells. The effects of (TQP)Sn(IV) and (TQC)Sn(IV) are more expressed than effects of free bases.

scholarly journals Phenolic compounds, antioxidant and antimicrobial properties of the wild cherry (Prunus avium L.) stem

2017 ◽  
pp. 1-13 ◽  
Author(s):  
Zahida Ademovic ◽  
Snjezana Hodzic ◽  
Zarka Halilic-Zahirovic ◽  
Darja Husejnagic ◽  
Jasna Dzananovic ◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
Prunus Avium ◽  
Antibacterial Properties ◽  
Antimicrobial Properties ◽  
Total Phenolic ◽  
Alcoholic Extract ◽  
Aqueous Extracts ◽  
Wild Cherry ◽  
Gram Positive Bacteria ◽  
Prunus Avium L

The aim of this study was to determine the total phenolic content, evaluate antioxidant propertie and antimicrobial potential, and identify phenolic compounds in alcoholic and aqueous extracts of the wild cherry (Prunus avium L.) stems collected in Bosnia and Herzegovina. Alcoholic extracts had higher contents of phenolic and flavonoid components, as well as the antioxidant and ferric reducing antioxidant capacity in comparison to aqueous extracts. All extracts were characterized by HPLC analysis. Furthermore, for the first time, the antimicrobial properties of wild cherry stem extracts were evaluated. Quercetin and (+)-catechin were the main compounds identified in the alcoholic extract, followed by chlorogenic acid and rutin. Quercetin was also the major component detected in aqueous extracts. Besides, alcoholic extract showed better antibacterial properties against Staphylococcus aureus as a representative gram-positive bacteria than infusion, whereas none of the samples showed antibacterial activity against Escherichia coli and fungus Candida albicans.

scholarly journals Antimicrobial Properties of Water-Soluble Polysaccharides and Alcoholic Extracts of Laetiporus Sulphureus (Bull.) Murrill Mycelium and Development of Biotechnology for Its Production in Immobilized Culture on Bacterial Cellulose

2020 ◽  
Vol 65 (1-2) ◽  
pp. 10-14
Author(s):  
I. A. Gavryushina ◽  
T. I. Gromovykh ◽  
N. B. Feldman ◽  
S. V. Lutsenko ◽  
V. I. Ponomarenko ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Bacterial Cellulose ◽  
Synthetic Medium ◽  
Antibacterial Properties ◽  
Antimicrobial Properties ◽  
Water Soluble ◽  
Biologically Active ◽  
Aqueous Extracts ◽  
Gram Positive Bacteria ◽  
Immobilized Culture

The article discusses the development of a new method of producing Laetiporus sulphureus (Bull.) Murrill basidiomycete mycelium immobilized on a bacterial cellulose matrix. Mycelium contains biologically active compounds with antimicrobial activity against Gram-positive bacteria, including resistant staphylococcus. The aim of the work was to obtain immobilized mycelium by co-cultivation of L.sulphureus with the producer of bacterial cellulose Gluconacetobacter hansenii. The authors found that when co-culturing the basidial L.sulphureus strain with the bacterial cellulose producing G.hansenii strain, productivity increases by 3.2 times on H5/1 synthetic medium and by 1.9 times on natural Maltax-10 medium (concentration 5%). The resulting immobilized L.sulphureus mycelium has antibacterial properties; its aqueous extracts contain glucans.

scholarly journals Antimicrobial Properties of Copper Nanoparticles and Amino Acid Chelated Copper Nanoparticles Produced by Using a Soya Extract

2017 ◽  
Vol 2017 ◽  
pp. 1-6 ◽  
Author(s):  
I. DeAlba-Montero ◽  
Jesús Guajardo-Pacheco ◽  
Elpidio Morales-Sánchez ◽  
Rene Araujo-Martínez ◽  
G. M. Loredo-Becerra ◽  
...  
Keyword(s):  
Amino Acids ◽  
Antibacterial Activity ◽  
Copper Nanoparticles ◽  
Copper Ions ◽  
Antibacterial Properties ◽  
Antimicrobial Properties ◽  
Bacterial Cells ◽  
Microdilution Method ◽  
Diphenyltetrazolium Bromide ◽  
Specific Strain

This paper reports a comparison of the antibacterial properties of copper-amino acids chelates and copper nanoparticles againstEscherichia coli,Staphylococcus aureus, andEnterococcus faecalis. These copper-amino acids chelates were synthesized by using a soybean aqueous extract and copper nanoparticles were produced using as a starting material the copper-amino acids chelates species. The antibacterial activity of the samples was evaluated by using the standard microdilution method (CLSI M100-S25 January 2015). In the antibacterial activity assays copper ions and copper-EDTA chelates were included as references, so that copper-amino acids chelates can be particularly suitable for acting as an antibacterial agent, so they are excellent candidates for specific applications. Additionally, to confirm the antimicrobial mechanism on bacterial cells, MTT assay (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide) was carried out. A significant enhanced antimicrobial activity and a specific strain were found for copper chelates overE. faecalis. Its results would eventually lead to better utilization of copper-amino acids chelate for specific application where copper nanoparticles can be not used.

scholarly journals New Auranofin Analogs with Antibacterial Properties against Burkholderia Clinical Isolates

Antibiotics ◽  
2021 ◽  
Vol 10 (12) ◽  
pp. 1443
Author(s):  
Dustin Maydaniuk ◽  
Bin Wu ◽  
Dang Truong ◽  
Sajani H. Liyanage ◽  
Andrew M. Hogan ◽  
...  
Keyword(s):  
Burkholderia Cepacia ◽  
Galleria Mellonella ◽  
Therapeutic Potential ◽  
Antibacterial Properties ◽  
Antimicrobial Properties ◽  
Clinical Isolates ◽  
Burkholderia Cepacia Complex ◽  
Model Organisms ◽  
Gram Positive Bacteria ◽  
High Concentrations

Bacteria of the genus Burkholderia include pathogenic Burkholderia mallei, Burkholderia pseudomallei and the Burkholderia cepacia complex (Bcc). These Gram-negative pathogens have intrinsic drug resistance, which makes treatment of infections difficult. Bcc affects individuals with cystic fibrosis (CF) and the species B. cenocepacia is associated with one of the worst clinical outcomes. Following the repurposing of auranofin as an antibacterial against Gram-positive bacteria, we previously synthetized auranofin analogs with activity against Gram-negatives. In this work, we show that two auranofin analogs, MS-40S and MS-40, have antibiotic activity against Burkholderia clinical isolates. The compounds are bactericidal against B. cenocepacia and kill stationary-phase cells and persisters without selecting for multistep resistance. Caenorhabditis elegans and Galleria mellonella tolerated high concentrations of MS-40S and MS-40, demonstrating that these compounds have low toxicity in these model organisms. In summary, we show that MS-40 and MS-40S have antimicrobial properties that warrant further investigations to determine their therapeutic potential against Burkholderia infections.

Study of the Resistance of PVC Composite Materials Modified with Wollastonite Activated by QAS to Bacteria

2021 ◽  
Vol 899 ◽  
pp. 132-136
Author(s):  
Dilyara F. Sadykova ◽  
Elena M. Gotlib ◽  
Elena V. Perushkina ◽  
Alla G. Sokolova
Keyword(s):  
Quaternary Ammonium ◽  
Antibacterial Properties ◽  
Antimicrobial Properties ◽  
Quaternary Ammonium Salts ◽  
Ammonium Salts ◽  
Gram Negative Bacteria ◽  
Gram Positive ◽  
Gram Negative ◽  
Gram Positive Bacteria ◽  
Materials Used

Evaluation of the antibacterial properties of PVC materials used, in particular, in medicine, is becoming increasingly important due to increased sanitary and hygienic requirements. One of the ways to ensure the resistance of PVC materials to microorganisms is modification with wollastonite, the surface of which is activated by quaternary ammonium salts (QASs). QASs are known for their antimicrobial properties. The results obtained indicate that the basic composition of linoleum is somewhat more resistant to gram-positive bacteria than to gram-negative bacteria. The introduction of wollastonite into the PVC compound formulation increases the resistance to the studied groups of microorganisms. It can be concluded that the surface treatment of Mivoll 10-97 by QAS generally improves the bactericidal properties of the material. The results obtained are consistent with the literature data on the activity of cationic QACs in relation to gram-positive and gram-negative bacteria. Quaternary ammonium salts have the ability to be attracted to the outer surface of the negatively charged bacterial membrane due to the positive charge of their molecules.

scholarly journals Antibacterial activity of Euphorbia antiquorum latex

2017 ◽  
Vol 2 (02) ◽  
pp. 15-17
Author(s):  
A.A.J.P. Kumara ◽  
D. L. Jayratne ◽  
G. V. Samaranayake
Keyword(s):  
Antibacterial Activity ◽  
Methanol Extract ◽  
Methanolic Extract ◽  
Antibacterial Properties ◽  
Antimicrobial Properties ◽  
Gram Negative Bacteria ◽  
Small Tree ◽  
E Coli ◽  
Gram Positive Bacteria ◽  
And Staphylococcus Aureus

Euphorbia antiquorum is a large shrub or small tree belonging to the largest and the most diverse family in the plant kingdom, Euphorbiaceae. E. antiquram latex was used as a classical binding agent for the kshara sutra in the management of the anal fistula. In the present study, an attempt was made to evaluate the antimicrobial properties of latex of E. antiquorum on certain microbes. The methanolic extract of the latex was prepared. The fractions of methanol extract were tested for their antibacterial activity against the Gram positive bacteria, Streptococus agalactiae and Staphylococcus aureus and Gram negative bacteria Escherichia coli and P. aeruginosa. The results revealed that the latex showed minimum inhibition only to E. coli and S. aureus. Latex did not posses antibacterial activity against S. agalactiae and P. aeruginosa. The results suggest that the E. antiquorum latex extract account for the antibacterial properties and has a potential for use as an antimicrobial agent.

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