Influence of acetaminophen consumption and exercise on Achilles tendon structural properties in male Wistar rats

2012 ◽  
Vol 302 (8) ◽  
pp. R990-R995 ◽  
Author(s):  
Chad C. Carroll ◽  
Jamie A. Whitt ◽  
Amity Peterson ◽  
Brian S. Gump ◽  
Jamie Tedeschi ◽  
...  
Keyword(s):  
Achilles Tendon ◽  
Water Content ◽  
High Performance ◽  
Wistar Rats ◽  
Collagen Content ◽  
Cross Linking ◽  
Tissue Water ◽  
Tendon Stiffness ◽  
Main Effect ◽  
Male Wistar Rats

Chronic consumption of acetaminophen (APAP) during exercise training leads to a reduction in tendon stiffness and modulus compared with a placebo. We explored whether this effect could be due to a reduction in tendon collagen content or cross-linking. Ten-week-old male Wistar rats ( n = 50) were divided into placebo or APAP groups and into sedentary or treadmill-exercised groups. APAP (200 mg/kg) or saline was administered once daily by oral gavage. Rats in the exercise groups ran on a treadmill 5 days per week for 8 wk with progression to 60 min per day, 20 m/min, and 8° incline. After 8 wk, lyophilized Achilles tendon samples were assayed for the collagen-specific amino acid hydroxyproline and cross-linking [hydroxylyslpyridinoline (HP)] content by high-performance liquid chromatrography. Collagen content was not influenced by exercise or APAP ( P > 0.05). Compared with placebo, tendon water content was 7% ( P = 0.006, main effect) lower in animals consuming APAP (placebo: 54.79 ± 0.8%, APAP: 50.89 ± 1.2%). HP in the Achilles tendon was 36% greater (sedentary: 141 ± 15, exercise: 204 ± 26 mmol/mol collagen) in the exercise-trained rats independent of drug treatment ( P = 0.020, main effect). Independent of exercise, HP content was 33% lower ( P = 0.032, main effect) in the animals consuming APAP (placebo: 195 ± 21, APAP: 140 ± 19 mmol/mol collagen). Our data suggests that chronic consumption of APAP results in a reduction in collagen cross-linking and a loss of tissue water independent of chronic exercise. This reduction in cross-linking and water content could contribute to the decrease in tendon stiffness noted in humans chronically consuming APAP.

scholarly journals Local treatment with ascorbic acid accelerates recovery of post-sutured Achilles tendon in male Wistar rats

2019 ◽  
Vol 52 (9) ◽  
Author(s):  
M. Souza ◽  
S.A.S. Moraes ◽  
D.R. de Paula ◽  
A.A. Maciel ◽  
E.J.O. Batista ◽  
...  
Keyword(s):  
Ascorbic Acid ◽  
Achilles Tendon ◽  
Wistar Rats ◽  
Local Treatment ◽  
Male Wistar Rats

scholarly journals Human Achilles tendon glycation and function in diabetes

2016 ◽  
Vol 120 (2) ◽  
pp. 130-137 ◽  
Author(s):  
Christian Couppé ◽  
Rene Brüggebusch Svensson ◽  
Mads Kongsgaard ◽  
Vuokko Kovanen ◽  
Jean-Francois Grosset ◽  
...  
Keyword(s):  
Achilles Tendon ◽  
Plantar Pressure ◽  
Foot Ulcers ◽  
Cross Linking ◽  
Diabetic Patients ◽  
Foot Pressure ◽  
Tendon Stiffness ◽  
Material Stiffness ◽  
Skin Collagen ◽  
Collagen Cross Linking

Diabetic patients have an increased risk of foot ulcers, and glycation of collagen may increase tissue stiffness. We hypothesized that the level of glycemic control (glycation) may affect Achilles tendon stiffness, which can influence gait pattern. We therefore investigated the relationship between collagen glycation, Achilles tendon stiffness parameters, and plantar pressure in poorly ( n = 22) and well ( n = 22) controlled diabetic patients, including healthy age-matched (45–70 yr) controls ( n = 11). There were no differences in any of the outcome parameters (collagen cross-linking or tendon stiffness) between patients with well-controlled and poorly controlled diabetes. The overall effect of diabetes was explored by collapsing the diabetes groups (DB) compared with the controls. Skin collagen cross-linking lysylpyridinoline, hydroxylysylpyridinoline (136%, 80%, P < 0.01) and pentosidine concentrations (55%, P < 0.05) were markedly greater in DB. Furthermore, Achilles tendon material stiffness was higher in DB (54%, P < 0.01). Notably, DB also demonstrated higher forefoot/rearfoot peak-plantar-pressure ratio (33%, P < 0.01). Overall, Achilles tendon material stiffness and skin connective tissue cross-linking were greater in diabetic patients compared with controls. The higher foot pressure indicates that material stiffness of tendon and other tissue (e.g., skin and joint capsule) may influence foot gait. The difference in foot pressure distribution may contribute to the development of foot ulcers in diabetic patients.

The effect of chronic treadmill exercise and acetaminophen on collagen and cross-linking in rat skeletal muscle and heart

2015 ◽  
Vol 308 (4) ◽  
pp. R294-R299 ◽  
Author(s):  
Chad C. Carroll ◽  
Karl Martineau ◽  
Kathryn A. Arthur ◽  
Richard T. Huynh ◽  
Brent D. Volper ◽  
...  
Keyword(s):  
Gastrocnemius Muscle ◽  
Treadmill Exercise ◽  
Dry Weight ◽  
Collagen Content ◽  
Cross Linking ◽  
Rat Skeletal Muscle ◽  
Oral Gavage ◽  
Specific Effects ◽  
Male Wistar Rats ◽  
Cross Links

The purpose of this study was to determine whether exercise and/or acetaminophen (APAP) alter collagen and cross-linking in the rat gastrocnemius muscle, soleus muscle, and heart. Male Wistar rats ( n = 50; 8 wk old) were divided into placebo (PLA) or APAP groups and sedentary (SED) or exercised (RUN) groups. APAP (200 mg/kg) was administered daily by oral gavage. Exercised groups ran on a treadmill 5 days/wk for 8 wk with progression to 60 min/day, 20 m/min, and 8° incline. Tissues were assayed for collagen (hydroxyproline) and hydroxylyslpyridinoline (HP) and lysylpyridinoline (LP) cross-links by HPLC. Collagen content (μg/mg dry weight) was greater in both the gastrocnemius (SED-PLA: 114 ± 16 vs. RUN-PLA: 244 ± 32; P < 0.001) and soleus (SED-PLA: 51 ± 7 vs. RUN-PLA: 99 ± 27; P = 0.005) of exercised animals. In contrast, collagen content was not significantly greater in exercised animals treated with APAP (SED-APAP: 113 ± 16 vs. RUN-APAP: 145 ± 21) and soleus (SED-APAP: 55 ± 8 vs. RUN-APAP: 57 ± 10). HP cross-linking (mmol/mol collagen) in the gastrocnemius (SED-PLA: 126 ± 28, RUN-PLA: 50 ± 7, SED-APAP: 41 ± 7, and RUN-APAP: 30 ± 4) and soleus muscles (SED-PLA: 547 ± 107, RUN-PLA: 318 ± 92, SED-APAP: 247 ± 64, and RUN-APAP: 120 ± 17) was lower in exercised rats compared with sedentary rats ( P < 0.05). Cross-linking was further reduced in animals treated with APAP ( P < 0.05). Neither heart collagen nor cross-linking was influenced by exercise or APAP ( P > 0.05). Our findings suggest that exercise and APAP have tissue-specific effects on muscle collagen. Given the widespread use of APAP as an analgesic and antipyretic, further work in humans is warranted.

scholarly journals Effect of postictal process in motor deficit and monoaminergic concentration in hippocampus, cerebellum, and cortex

Salud Mental ◽  
2019 ◽  
Vol 42 (5) ◽  
pp. 251-256
Author(s):  
Alberto Avila-Luna ◽  
Antonio Bueno-Nava ◽  
José Luis Cortes-Altamirano ◽  
Samuel Reyes-Long ◽  
Cindy Bandala ◽  
...  
Keyword(s):  
High Performance ◽  
Wistar Rats ◽  
Saline Solution ◽  
Motor Behavior ◽  
Brain Structures ◽  
Motor Deficit ◽  
Motor Deficits ◽  
Male Wistar Rats ◽  
Neurochemical Changes ◽  
Experimental Group

Introduction. Systemic administration of pentylenetetrazole (PTZ) causes brain damage (BD), and triggers a series of morphological and neurochemical changes, which in turn bring about behavioral, cognitive, and motor deficits. Serotonin (5-HT), dopamine (DA), and noradrenaline (NA) levels are controlled by various brain structures and these levels are related to motor activity; however, the concentration of these neurotransmitters during the postictal process remains unknown. Objective. We investigated the concentration of 5-HT, NA and DA in the hippocampus, cerebellum, and cortex on motor deficit during the postictal stage. Method. Eighteen male Wistar rats (300 g) assigned to two groups: control (n = 9, saline solution) and experimental (n = 9, PTZ) were used. Myoclonic shakes were counted and motor behavior assessments were recorded during three hours post PTZ injection (90 mg/kg). The cortex, cerebellum, and hippocampus of each rat were dissected to determine the 5-HT, DA, and NA concentration by high performance liquid chromatography. Results. PTZ induced a significant increase in total 5-HT and DA levels in the hippocampus and cortex; in the cerebellum there was a significant increase in the concentration of 5-HT and NA. The presence of myoclonic shakes as well as a marked motor deficit in the experimental group were significantly different in comparison to the control. Discussion and conclusion. 5-HT modifies the concentration of other monoamines directly involved in motor aspects such as NA and DA in the hippocampus, cerebellum, and cortex during the postictal process.

scholarly journals Pengaruh Bubur Pisang Isomaltosa-oligosakarida dan Fibercreme terhadap Kadar Glukosa dan Lipida Darah serta Profil Digesta Tikus Diabetes

2020 ◽  
Vol 40 (3) ◽  
pp. 190
Author(s):  
Yustinus Marsono ◽  
Priyanto Triwitono ◽  
Elisabeth Desy Arianti ◽  
Hendrik Gunawan ◽  
Rochmad Indrawanto
Keyword(s):  
Water Content ◽  
Dietary Fiber ◽  
Glucose Level ◽  
Triglyceride Level ◽  
Resistant Starch ◽  
Wistar Rats ◽  
Serum Glucose ◽  
Standard Diet ◽  
Lipid Concentration ◽  
Male Wistar Rats

Dietary fiber and resistant starch are known to be beneficial in lowering both glucose and lipid concentration in human. Fibercreme, a fiber rich product has been developed. It is a commercial non-dairy creamer that uses various oligosaccharides such as Isomaltose-oligosaccharides (IMO) or inulin as fiber source to replace the glucose component of conventional creamer. In this study, IMO and fibercreme were used to replace sucrose of the banana porridge. Therefore, the purpose of this study was to determine porridge effects on glucose, triglyceride, serum cholesterol and digesta profile in diabetic STZ-NA induced rats. Twenty-five male Wistar rats were divided into five groups and fed four different diets including the standard (KS and KN), banana porridge with sweetener IMO (BP+IMO), fibercreme (BP+FC) and (BP + Sukrosa). In these diets, porridge subtituted 30% energy of the standard diet, and the intervention was conducted for 28 days. It was found that banana-IMO porridge showed the lowest decrease in serum glucose level with 56.09%, followed by banana-fibercreme (47.61%) and bananasucrose (36.61%). Furthermore, similar trend was found in their cholesterol-lowering effects with 36.82%, 25.68% and 7.86% respectively. However, triglyceride level effects were highest in banana-IMO porridge (9.06%), followed by banana-sucrose (6.47%) and banana-fibercreme (3.72%). In addition, sucrose replacement with IMO increases weight and water content, as well as decrease caecal digesta pH. However, fibercreme increases only water content and does not decrease caecal digesta pH.

scholarly journals ACETAMINOPHEN CONSUMPTION ALTERS ACHILLES TENDON COLLAGEN CONTENT IN WISTAR RATS

2010 ◽  
Vol 24 (S1) ◽  
Author(s):  
Jamie A Whitt ◽  
Jamie Tedeschi ◽  
David R McMullan ◽  
Jonathan D Del Mundo ◽  
Tom L Broderick ◽  
...  
Keyword(s):  
Achilles Tendon ◽  
Wistar Rats ◽  
Collagen Content ◽  
Tendon Collagen

scholarly journals TEST OF ANTI-INFLAMATION ACTIVITIES OF PEPAYA LEAF (Carica papaya L.) EXTRACT ON MALE WISTAR RATS INDUCED BY CARAGENAN 1%

2019 ◽  
Vol 19 (3) ◽  
pp. 54-57
Author(s):  
Fenny Hasanah ◽  
Nurul Hidayah
Keyword(s):  
Water Content ◽  
Wistar Rats ◽  
Carica Papaya ◽  
Water Extract ◽  
Test Group ◽  
Leaf Water ◽  
Inflammatory Activity ◽  
Phytochemical Screening ◽  
Male Wistar Rats ◽  
Anti Inflammatory

Inflammation is a normal protective response to tissue injuries caused by physical trauma, destructive chemicals, or microbiological substances. The purpose of this study was to determine the anti-inflammatory activity of papaya leaf water extract (Carica papaya L.) on the feet of male Wistar rats which were induced by carrageenan 1%. The research method used is experimental. The stages of this study include plant identification, making dry simplicia powder, simplicia characteristics, extracting papaya leaves using water, simplicia phytochemical screening and papaya leaf water extract (EADP), as well as testing for anti-inflammatory activity. Anti-inflammatory testing was carried out on 20 male Wistar rats induced with 1% carrageenan sub plantar. The test group was divided into five groups for the induction, comparison, EADP 50, 100, 200 mg/kg BW groups. The parameters observed were the volume of mouse leg udema measured by a pletismometer every 30 min for 5 h. Data obtained then calculated percent inflammation and percent inflammation inhibition. Furthermore, it was analysed by ANOVA with a confidence level of 95%. The results of the simplicia characteristics showed that the water content, water content of the sea water and soluble extracts of ethanol were 8.9%, 51.4% and 10.2% respectively. The results of simplicia and EADP phytochemical screening showed the content of flavonoids, tannins, triterpenoids, glycosides, and alkaloids. Observations of the percentage of inflammation in the induction group had the highest percentage of inflammation compared with the other test groups at time of 300 min (p 0.05). Whereas the percentage of inflammatory inhibition of all groups of test extracts has effectiveness comparable to the comparison group. EADP group 200 mg/kg BW is the best dose of extract as anti-inflammatory, where flavonoid compounds are thought to be responsible for decreasing the volume of inflammation in the legs of test animals.

scholarly journals Profil Kadar Kolagen Kulit dan Tulang Tikus Wistar pada Berbagai Umur yang Mendapat Perlakuan Stres Oksidatif Hiperkolesterolemia dan Oleoresin Kulit Batang Kayu Manis (Cinnamomum sp)

2012 ◽  
Vol 12 (2) ◽  
pp. 56
Author(s):  
Sunarno Sunarno ◽  
Sri Isdadiyanto
Keyword(s):  
Oxidative Stress ◽  
Wistar Rats ◽  
Age Groups ◽  
Aging Effect ◽  
Cholesterol Content ◽  
Collagen Content ◽  
Bone Collagen ◽  
Treatment Groups ◽  
Skin Collagen ◽  
Male Wistar Rats

Oleoresin cinnamon bark belonging to the phenolic compounds that are known to have potential as antioxidants. On the basis of the potential of these compounds may be used to prevent or repair tissue damage, whether caused by the factors of age and condition of oxidative stress hypercholesterolemia. This study aimed to obtain skin and bone collagen profile of Wistar rats at various ages after oxidative stress treated hypercholesterolemia and oleoresin from the bark of cinnamon (Cinnamomum sp). This study used 54 male Wistar rats with body weight (200 ± 5 g), which are grouped into six treatment groups combined with the three age groups of mice that is three, six or nine months with three replications. Each group received treatment oleoresin and hypercholesterolemia. Giving oral oleoresin conducted on rats given oral way during the 7 days with a dose of 12 rats mg/200-gr bw / day. Hypercholesterolemic rats was conducted by feeding cholesterol content within 1% during the two months. The results showed that the profile of skin and bone collagen content of the highest found on threemonth old rats treated with oleoresin, oleoresin hypercholesterolemia and given again (P6, 2), namely 57.44 tg / mg and 33.47 tg / mg, while the profile of skin and bone collagen content of the lowest found in rats aged 9 months to get treatment without treatment of hypercholesterolemia oleoresin (P2, 9), namely 28.26 tg / mg and 10.65 tg / mg. From this research can be concluded that the condition of hypercholesterolemia and duration of the aging effect on skin collagen levels decrease and bone, and vice versa oleoresin at the age of young rats that received the treatment of oxidative stress could repair or prevent hypercholesterolemia decreased content of skin collagen and bone. In general, the rats are treated oleoresin and young age profile of the collagen content of skin and bones better compared with rats that do not get treatment in conditions of oxidative stress oleoresin hypercholesterolemia.

Suppressed serum prolactin in sinoaortic-denervated rats

1987 ◽  
Vol 252 (2) ◽  
pp. R290-R293
Author(s):  
N. Alexander ◽  
S. Melmed ◽  
M. Morris
Keyword(s):  
Heart Rate ◽  
Anterior Pituitary ◽  
High Performance ◽  
Wistar Rats ◽  
Serum Prolactin ◽  
Posterior Pituitary ◽  
Before And After ◽  
Male Wistar Rats ◽  
Pituitary Prolactin ◽  
Arterial Baroreceptor

We investigated the effect of arterial baroreceptor deafferentation on serum and pituitary prolactin (PRL) and on catecholamines in median eminence (ME) and anterior and posterior pituitaries. Male Wistar rats were sinoaortic denervated (SAD) or sham operated (SO). Three days after surgery serum prolactin, measured by radioimmunoassay, was suppressed in SAD rats (-54%, P less than 0.05), and dopamine (DA) and norepinephrine (NE) concentrations, measured by radioenzymatic or high-performance liquid chromatography electron capture methods, were significantly reduced in ME of SAD rats (NE, -54% P less than 0.005 and DA, -56% P less than 0.001). Simultaneously, anterior pituitary of SAD rats had significant increases in both catecholamines, whereas posterior pituitary showed no changes. Four hours after surgery serum PRL was also reduced (-40%, P less than 0.05) in SAD rats, but no changes in ME catecholamines were found. Mean arterial pressure (MAP) and heart rate were measured before and after injection of bromocriptine (0.5 mg/kg ip) in SAD and SO rats 3 days after surgery. Bromocriptine markedly suppressed serum PRL in both groups and reduced MAP from 144 +/- 10 to 84 +/- 5 and from 116 +/- 2 to 99 +/- 3 in SAD and SO rats, respectively; heart rate was reduced in SAD rats. We conclude that the SAD rat is a model of hypertension with suppressed serum PRL and that interruption of arterial baroreceptor nerves suppresses PRL secretion probably by modulating tuberoinfundibular turnover of catecholamines.

Structural and functional renal alterations in five-sixth nephrectomized rats on unrestricted diet

1986 ◽  
Vol 44 ◽  
pp. 342-343
Author(s):  
I. Stachura ◽  
M. Pardo ◽  
J. Costello ◽  
D.M. Landwehr
Keyword(s):  
Wistar Rats ◽  
Renal Damage ◽  
Standard Diet ◽  
Experimental Conditions ◽  
Phosphate Intake ◽  
Dietary Restrictions ◽  
Severe Reduction ◽  
Male Wistar Rats ◽  
Unrestricted Diet ◽  
Progressive Renal Insufficiency

Under experimental conditions severe reduction of renal mass results in the hyperfiltration of the remaining nephrons leading to a progressive renal insufficiency. Similar changes are observed in patients with various renal disorders associated with a loss of the functioning nephrons. The progression of renal damage is accelerated by high protein and phosphate intake, and may be modified by the dietary restrictions.We studied 50 five-sixth nephrectarrized male Wistar rats on a standard diet (Rodent Laboratory Chow 5001 Ralston Purina Co., Richmond, Indiana; containing 23.4% protein) over a 20 week period.

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