Electrophysiology using coaxial atom probe array: Live imaging reveals hidden circuits of a hippocampal neural network

Author(s):  
Pushpendra Singh ◽  
Komal Saxena ◽  
Pathik Sahoo ◽  
Subrata Ghosh ◽  
Anirban Bandyopadhyay

Since the 1960s, it is held that when a neuron fires, a nerve spike passes only through the selective branches, the calculated choice is a key to learning by rewiring. It is argued by chemically estimating the membrane's ion channel density that different axonal branches get active to pass the spike -branches blink at firing at different time domains. Here, using a new time-lapse dielectric imaging, we visualize the classic branch selection process, hidden circuits operating at different time domains become visible. The fractal grid of coaxial probes captures wireless snapshots of material's vibration at various depths below the membrane by setting a suitable frequency. Thus far, branch selection observed emitted energy or particle but never the emitters, what they do. Since each dielectric material transmits & reflects signals of different frequencies, we image live how filaments search for many branch-made-circuits, choose an unique pathway 103 times faster than a single nerve spike. It reveals that neural branches and circuit visible in a microscope is not absolute, there coexist many circuits each operating in different dime domains, operating at a time.

2021 ◽  
Author(s):  
Fernando Mata

In the last decades, migration from Latin America to Canada has become a topic of interest for Canadian scholars, policy decision-makers that look after the well-being of this population as well as for community members themselves. The nature of Latino immigration to Canada is continuously changing, and so does how the integration of these immigrants to Canada is interpreted and problematized. Using yearly immigration statistics and 2016 Census data, the author looks at the 1965-2015 and 1981-2016 periods and explores the five major Latin American immigrant waves previously identified by Canadian scholars: the Eurolatino or Lead of the 1960s, the Andean and Coup of the 1970s, the Central American of the 1980s, and the Technological-Professional which started in the mid-1990s. A sixth additional Sustaining Latino immigrant wave is also identified. Immigrant waves are the product of particular historical international developments as well as changes in Canada's immigration policy. The paper briefly also examines the historical moments of Latino immigration to Canada, the socio-demographic composition of national immigrant inflows related to these immigrant waves, and reflects on how the immigrant selection process has affected immigration integration outcomes and community formation.


Author(s):  
Anna Elisa de Villemor-Amaral

The Rorschach request for candidates for positions in the upper corporate hierarchy – the so-called executive positions – has become a usual practice since the 1960s when the most classic and complete work about executive evaluation with the Rorschach appeared. The most commonly cited traits for successful executives are: ambition and intense desire to achieve; ability to make decisions; assertiveness; practicality; ability to work efficiently in frustrating conditions; ability and desire to include others in solving problems; ability to question his/her own positions and decisions in an objective fashion; ability to express hostility with tact; ability to set realistic targets; have varied tastes; “ability to work most of the time at a dizzying pace and sometimes with the reflection and calm of a Buddhist monk” (p. 4); interest in others; ability to deal with concrete and abstract problems; ability to be cautious or bold depending on circumstances; ability to take positions; initiative; predictive capacity; security; emotional stability; honesty; integrity; dedication; objectivity and cooperation. The study involved 20 Rorschach protocols of executives, all of them employed but in competition for positions in the direction of international or multinational companies, which were assessed as part of the selection process. The results do not match the profile expected. The high frequencies of Zd < –3 given by 10 executives (50%) show a hasty attitude for analyzing facts and a tendency to draw rash and careless conclusions, which increases the risk of error in making a decision. Furthermore, 7 subjects (35%) showed a high p, which reveals a tendency to a more passive attitude, expecting that others will solve the problem, and acting according to the desires of others, which can indicate feeling uncomfortable as leaders and avoiding responsibilities. In addition, 50% of the group presented Fd responses, and in four of them (20%) the food responses were accompanied by a < p, and two of them also showed an association with T > 1, a triad indicating dependency. Concerning the AdjD, 55% of our sample presented a negative score – meaning lack of resources for coping in stress situations and little ability to confront new problems. There is a high incidence of DEPI. The presence of DEPI greater than or equal to 5 in 50% of the group is surprising in executives and directors. The CDI is also positive in 50% of the subjects, which means difficulties coping with complex social situations. The lack of relational skills can create adaptation problems, make people more vulnerable to stress, and is a precursor to secondary depression. In the group, six individuals presented values of S > 3, five showed S = 3, and none of them showed S only in the first three cards. These data reflect oppositionist tendencies and aggressiveness that is manifested in making decisions and in negativist and obstinate ways of relating to others, or the manner of seeing the world, understanding situations, and confronting the environment with feelings of rage. With the exception of the high number of responses, which denote high productivity, most of the indicators we found would not allow the prediction of professional success, especially as concerns characteristics of depression, relational deficit, and passive dependency. The fact that many among the executives examined presented rather good curriculum vitae and were, thus, sent for the assessment, is interesting.


2018 ◽  
Author(s):  
Vera Fritz

Drawing on archives gathered in the six founding member states of the European Union, this book offers a new perspective on the ‘constitutionalization’ of the European treaties, which was launched in the 1960s by the Court of Justice of the EU. By highlighting the professional and personal backgrounds of the first European judges and advocates general, its author studies the dynamics which prevailed within the institution during its ‘revolutionary’ years and analyzes the Court’s relationship with the Member States of the European Communities. Through a detailed study of the selection process of the members of the Court, it also provides new answers to the question of whether governments tried to put an end to its bold jurisprudence.


Author(s):  
Matthew N Green ◽  
Douglas Harris

How are congressional party leaders chosen? In the first comprehensive study since Robert Peabody's classic Leadership in Congress, this book draws on newly collected data about U.S. House members who have sought leadership positions from the 1960s to the present—data including whip tallies, public and private vote commitments, interviews, and media accounts—to provide new insights into how the selection process truly works. Elections for congressional party leaders are conventionally seen as a function of either legislators' ideological preferences or factors too idiosyncratic to permit systematic analysis. Analyzing six decades' worth of information, the book finds evidence for a new comprehensive model of vote choice in House leadership elections that incorporates both legislators' goals and their connections with leadership candidates.


2019 ◽  
Vol 16 (1) ◽  
pp. 75
Author(s):  
Irfandhani Fauzi ◽  
Kahfiati Kahdar ◽  
Slamet Riyadi

<p><strong>Abstract</strong></p><p><strong></strong><br />The Majalaya textile industry, at Bandung Regency, is a textile centre prepared by the government as a national textile centre to meet clothing needs. This industry was able to develop and experience its heyday in the 1960s. At that time, this region was able to produce 40% of the total fabric production in Indonesia. Sarong, as its main product, was very popular throughout Indonesia and several other countries in Asia. But the<br />crisis that occurred in 1998 had a major impact on the textile industry in Majalaya. Many textile entrepreneurs are forced to close their production sites because of the declining purchasing power of the people and also the increasing cost of raw material for production. Only a handful of Majalaya sarong entrepreneurs have survived until now. In the midst of their efforts to survive, the problem that occurs at this time is the onslaught of imported textiles that flooded the market which impacted the sale of<br />Majalaya sarongs falling dramatically.These problems must be overcome with the aim of returning the Majalaya textile industry as the people’s economic power. Design in relation to this is aimed at producing products that are interested in consumers. Design is an important aspect of product development, also as one of the main criteria in the product selection process by buyers. To produce an interesting product it is necessary<br />to analyze consumer perceptions. The purpose is to know what aspects that affect consumer interest in buying products. These can be applied as a strategy for developing a product of Majalaya sarong. The survey method using a questionnaire to Majalaya sarong consumers can be done to determine consumer perceptions of Majalaya sarong products. By analyzing consumer perceptions, producers can develop a Majalaya sarong<br />product design strategy that suits consumer interest.</p><p> </p><p><br /><strong>Abstrak </strong><br />Industri tekstil Majalaya, di Kabupaten Bandung, merupakan sentra tekstil yang disiapkan pemerintah sebagai pusat tekstil nasional guna memenuhi kebutuhan sandang. Industri ini mampu berkembang dan mengalami puncak kejayaannya pada tahun 1960-an. Saat itu, wilayah ini mampu memproduksi 40% dari total produksi kain di Indonesia. Sarung, sebagai produk utamanya, sangat popular di seluruh Indonesia serta beberapa Negara di Asia. Namun krisis yang terjadi pada tahun 1998 berdampak besar bagi industri tekstil di Majalaya. Banyak pengusaha tekstil yang terpaksa menutup tempat produksinya karena daya beli masyarakat yang menurun dan juga kenaikan biaya bahan baku produksi. Hanya segelintir pengusaha sarung Majalaya yang masih bertahan menjalankan usahanya hingga sekarang. Di tengah usahanya untuk bertahan, permasalahan yang juga dialami pengusaha tekstil Majalaya saat ini adalah gempuran tekstil impor yang membanjiri pasar yang mengakibatkan penjualan sarung Majalaya turun drastis.Permasalahan yang dialami industri tekstil Majalaya tersebut harus diatasi dengan tujuan untuk mengembalikan industri tekstil Majalaya sebagai penggerak ekonomi rakyat. Peran desain dalam kaitannya dengan hal ini bertujuan untuk menghasilkan produk yang diminati masyarakat. Desain merupakan aspek yang penting bagi pengembangan produk, juga sebagai salah satu kriteria utama dalam proses pemilihan produk oleh pembeli. Salah satu cara yang dapat dilakukan untuk menghasilkan produk yang diminati adalah dengan menganalisis persepsi konsumen. Hal ini dilakukan karena tujuan dari analisis persepsi konsumen adalah untuk mengetahui aspek-aspek apa saja yang mempengaruhi minat konsumen untuk membeli produk. Aspek-aspek tersebut dapat diterapkan sebagai strategi untuk pengembangan produk sarung Majalaya. Metode survey dengan menggunakan kuisioner kepada konsumen sarung Majalaya dapat dilakukan untuk mengetahui persepsi konsumen terhadap produk sarung Majalaya. Dengan menganalisis persepsi konsumen, maka produsen dapat membuat strategi desain produk sarung Majalaya yang sesuai dengan minat konsumen.</p>


2001 ◽  
Vol 32 ◽  
pp. 268-274 ◽  
Author(s):  
E. Suriñach ◽  
G. Furdada ◽  
F. Sabot ◽  
B. Biesca ◽  
J. M. Vilaplana

AbstractSeismic signals from artificially released avalanches were studied in an attempt to characterize them for avalanche-monitoring purposes. The seismic signals generated by different sizes and types of avalanches were recorded and analyzed in the time and frequency domains. Synchronized recordings of the corresponding seismic signals and the video images of the evolution of the avalanches were obtained together with a detailed cartography. Characteristic signatures in the frequency and time domains were found to depend on the characteristics of the avalanche path and measuring location, but to be mostly independent of avalanche size. The source of the different parts of the recorded seismic signals was determined. A relationship was observed between the avalanche size and the amplitude of the signals. Given the presence of local site effects, a prior seismic characterization of the avalanche path in relation to the recording sites is necessary for monitoring purposes. Moreover” it was found that sliding slabs in the early phase of acceleration produce little seismic energy, resulting in a time lapse between the observable start of the avalanche and the arrival of the detectable seismic waves at the receiving station.


2020 ◽  
Author(s):  
Thomas Higginbottom ◽  
Roshan Adhikari ◽  
Ralitza Dimova ◽  
Sarah Redicker ◽  
Timothy Foster

&lt;p&gt;One of the most controversial topics within the African food-water-energy nexus is the development of dams and large-scale irrigation schemes. Colonial authorities began constructing these schemes in the early 20&lt;sup&gt;th&lt;/sup&gt; century, with construction accelerating in the 1960s helped by support from the World Bank and multi-lateral institutions. However, over the following decades evidence of the environmental, financial and social costs incurred by dams began to mount, leading to a hiatus on new developments by the early 1990s. Yet this pause is now ending, and many new dams and large-scale schemes are planned for Africa and Asia. It is therefore essential that mistakes of previous eras be avoided.&lt;/p&gt;&lt;p&gt;In this study, we quantify how the size of irrigation schemes successfully delivered compares to the initial project proposals, and what factors contribute to any observed discrepancies.&amp;#160; We combined novel data on the size of proposals, obtained from planning documents, with satellite-derived cropland maps for 80 African irrigation schemes constructed between 1945 and 2008, from 23 nations. Firstly, We calculate what proportion of the initial proposal is successfully delivered. Secondly, we model these proportions against a suite of potential explanatory variables - covering hydroclimatic, socioeconomic, national, and site-specific factors- in a variable selection process.&lt;/p&gt;&lt;p&gt;We show that the average proportion of proposed irrigation successfully delivered is low, with a mean of 48% and a median of 18%. However, there was considerable variation in performance depending on the initial proposal size, with large schemes &amp;#8212; that propose over 10,000 ha &amp;#8212; proving far less efficient, delivering 10 - 20% and never reaching 100%. Smaller schemes performed slightly better, with 15 sites delivering over 80%. Hydro-climatic factors were not significant predictors of the successful delivery; contradicting narratives of drought-induced failure for many schemes.&amp;#160; However, national government effectiveness, as measure by the World Bank, was a significant predictor. There was no trend in the proportions of successful delivery over the study period, indicating a failure to learn from issues that arouse on previous schemes. We proposed that the underperformance of irrigation projects is driven by the following factors: 1) over optimistic proposals, which are unrealistically large in order to generate investment; 2) large schemes being overly complex, in technology and maintenance, and therefore being difficult to manage, and 3) governance capacity for developing and handling large investments being limited and hindered by inefficient bureaucracy .&lt;/p&gt;&lt;p&gt;Our findings highlight that major issues remain for large-scale irrigation development in Africa. If dams and large-scale irrigation schemes are to be a helpful component of future development strategies for poverty alleviation and food production, these issue require urgent attention.&lt;/p&gt;


2014 ◽  
Vol 38 (01) ◽  
pp. 102-129
Author(s):  
ALBERTO MARTÍN ÁLVAREZ ◽  
EUDALD CORTINA ORERO

AbstractUsing interviews with former militants and previously unpublished documents, this article traces the genesis and internal dynamics of the Ejército Revolucionario del Pueblo (People's Revolutionary Army, ERP) in El Salvador during the early years of its existence (1970–6). This period was marked by the inability of the ERP to maintain internal coherence or any consensus on revolutionary strategy, which led to a series of splits and internal fights over control of the organisation. The evidence marshalled in this case study sheds new light on the origins of the armed Salvadorean Left and thus contributes to a wider understanding of the processes of formation and internal dynamics of armed left-wing groups that emerged from the 1960s onwards in Latin America.


Author(s):  
Raul I. Garcia ◽  
Evelyn A. Flynn ◽  
George Szabo

Skin pigmentation in mammals involves the interaction of epidermal melanocytes and keratinocytes in the structural and functional unit known as the Epidermal Melanin Unit. Melanocytes(M) synthesize melanin within specialized membrane-bound organelles, the melanosome or pigment granule. These are subsequently transferred by way of M dendrites to keratinocytes(K) by a mechanism still to be clearly defined. Three different, though not necessarily mutually exclusive, mechanisms of melanosome transfer have been proposed: cytophagocytosis by K of M dendrite tips containing melanosomes, direct injection of melanosomes into the K cytoplasm through a cell-to-cell pore or communicating channel formed by localized fusion of M and K cell membranes, release of melanosomes into the extracellular space(ECS) by exocytosis followed by K uptake using conventional phagocytosis. Variability in methods of transfer has been noted both in vivo and in vitro and there is evidence in support of each transfer mechanism. We Have previously studied M-K interactions in vitro using time-lapse cinemicrography and in vivo at the ultrastructural level using lanthanum tracer and freeze-fracture.


Author(s):  
M.P. Thomas ◽  
A.R. Waugh ◽  
M.J. Southon ◽  
Brian Ralph

It is well known that ion-induced sputtering from numerous multicomponent targets results in marked changes in surface composition (1). Preferential removal of one component results in surface enrichment in the less easily removed species. In this investigation, a time-of-flight atom-probe field-ion microscope A.P. together with X-ray photoelectron spectroscopy XPS have been used to monitor alterations in surface composition of Ni3Al single crystals under argon ion bombardment. The A.P. has been chosen for this investigation because of its ability using field evaporation to depth profile through a sputtered surface without the need for further ion sputtering. Incident ion energy and ion dose have been selected to reflect conditions widely used in surface analytical techniques for cleaning and depth-profiling of samples, typically 3keV and 1018 - 1020 ion m-2.


Sign in / Sign up

Export Citation Format

Share Document