scholarly journals Screening of Nutritional Parameters for the Production of Protease fromAspergillus Oryzae

2007 ◽  
Vol 4 (2) ◽  
pp. 208-215 ◽  
Author(s):  
G. Srinubabu ◽  
N. Lokeswari ◽  
K. Jayaraju
Keyword(s):  
Carbon Sources ◽  
Nitrogen Sources ◽  
Fold Increase ◽  
Protease Production ◽  
Malt Extract ◽  
Culture Time ◽  
Protease Enzyme ◽  
Bean Meal ◽  
Soya Bean Meal ◽  
Fungus Growth

Production of protease enzyme by fungusAspergillus oryzaewas investigated. The proteolytic activity was observed when the fungus was grown in the medium containing glucose, malt extract, yeast extract, peptone, K2HPO4, MgSO4and FeSO4. The present paper describes the screening of media components and fermentation conditions in shake flask. The organism utilized carbon sources glucose, fructose, sucrose, lactose, dextrin and starch among them glucose was found to be the best carbon source, for nitrogen sources various inorganic and organic media components were investigated among them peptone is found to be the best nitrogen source. 1% cottonseed followed by 2% Soya bean meal was found to be the best inducer. With optimized media two-fold increase in the protease production. The fungus growth depends on the concentration of carbon, nitrogen and salt solution, where as the enzyme production was also influenced by the culture time, pH and interaction between these two variables.

scholarly journals Protease Production from UV Mutated Bacillus subtilis

2012 ◽  
Vol 47 (1) ◽  
pp. 69-76 ◽  
Author(s):  
MG Sher ◽  
M Nadeem ◽  
Q Syed ◽  
M Irfan ◽  
S Baig
Keyword(s):  
Bacillus Subtilis ◽  
Inorganic Nitrogen ◽  
Carbon Sources ◽  
Nitrogen Sources ◽  
Fermentation Medium ◽  
Maximum Activity ◽  
Inoculum Size ◽  
Protease Production ◽  
Protease Enzyme ◽  
Uv Mutation

UV mutation of the strain has significant contributation to enhance the yield of protease enzyme from Bacillus subtilis bacteria under the  cultivation conditions in submerged fermentation. The fermentation medium used for the production of protease composed of carbon  sources 1%, organic 1% or inorganic nitrogen sources 0.5% , K2HPO4 0.2 %, CaCl2 0.04% and MgSO4 0.02 % by mutated Bacillus subtilis  G-4 under the optimum parameters which are important to induce the mutated strain to produce high units of the protease, which were  temperature 37.5°C, pH 9, inoculum size 3 % v/v, glucose 1% as carbon source and peptone 1% as nitrogen source were give the maximum  455.25 + 1.66 units of protease. The results of stability studies revealed that protease of B. subtilis G-4 was stable over a broad range  of temperature (30 to 60°C) and pH (8 to 12). However, maximum activity (155.45U/ml) was observed at temperature 50°C and pH 10.  These characteristics render its potential use in detergent industries for detergent formulation.DOI: http://dx.doi.org/10.3329/bjsir.v47i1.10725 Bangladesh J. Sci. Ind. Res. 47(1), 69-76, 2012

scholarly journals Study of Gibberellic Acid Production by Solid State Fermentation Using Fusarium Moniliforme Sheldon

2016 ◽  
Vol 4 (3) ◽  
pp. 402-407 ◽  
Author(s):  
Rakeshkumar Ramanlal Panchal ◽  
Piyushbhai Vishnubhai Desai
Keyword(s):  
Solid State ◽  
Gibberellic Acid ◽  
Solid State Fermentation ◽  
Acid Production ◽  
Fusarium Moniliforme ◽  
Carbon Sources ◽  
Nitrogen Sources ◽  
Fold Increase ◽  
Soluble Starch ◽  
Sugarcane Plant

Gibberellic acid production using Fusarium moniliforme, isolated from wilted sugarcane plant has been investigated by solid state fermentation (SSF). The gibberellic acid production of 154mgm/gm was obtained on commercial wheat bran (CWB) mineral salt acid bed in 500 ml flasks after 168 h incubation. The gibberellic acid production rate was about 0.6 to 0.9 mgm/gm/hr during 96 to 168 h. Different carbon sources namely sucrose, lactose, maltose, soluble starch, glycerol, wheat flour and maize flour were tested as an additional substrate along with CWB at the concentration of 25% w/w or v/w base to observe its effects on gibberellic acid production. Soluble starch has been proved the best additional carbon source for gibberellic acid production, which yielded 1160mgm/gm of gibberellic acid after 168 h. Similarly, various nitrogen sources namely NH4Cl, NH4NO3, (NH4)2SO4, (NH4)MoO4 and urea were tested as an additional substrate at the concentration of 0.07% w/w of CWB. Urea was proved as the best nitrogen source which yielded 532 mgm/gm of gibberellic acid after 168 h incubation. We have observed about 7.5-fold and 3.5-fold increase in gibberellic acid production upon addition of soluble starch and urea respectively, in CWB using Fusarium moniliforme.Int J Appl Sci Biotechnol, Vol 4(3): 402-407

scholarly journals Keratinolytic Protease Production by Bacillus Cereus Strain Ps03 under Submerged Fermentation: Optimization and Characterization

2016 ◽  
Vol 4 (3) ◽  
pp. 397-401
Author(s):  
M.D. BalaKumaran ◽  
R. Santhi
Keyword(s):  
Bacillus Subtilis ◽  
Scale Up ◽  
Nitrogen Sources ◽  
Maximum Activity ◽  
Protease Production ◽  
Subtilis Strain ◽  
Physical Factors ◽  
Chicken Feather ◽  
Protease Enzyme ◽  
Keratinolytic Protease

In the present study, chicken feather powder was screened for its application as the substrate for the production of keratinolytic protease by Bacillus subtilis strain PS03. Bacillus subtilis produced a high level of keratinolytic protease using chicken feather powder as substrate. With feather powder as substrate, physical factors such as incubation time, pH and temperature were optimized for increased keratinolytic protease production by Bacillus subtilis. The enzyme production was enhanced when using maltose as carbon source and yeast extract as nitrogen sources. SDS-PAGE analysis indicated the molecular weight of 46 kDa of the partially purified keratinolytic protease. The keratinolytic protease enzyme was stable over a pH range of 6 – 9 and temperature range of 35 - 50°C with maximum activity at pH 9 and 40°C. Based on the results, the use of feather powder as substrate for keratinolytic protease production is cost effective and is easy to scale up. Considering the availability and cost, chicken feather powder is considered as an ideal substrate for keratinolytic protease production in an industrial point of view. Int J Appl Sci Biotechnol, Vol 4(3): 397-401

scholarly journals A Novel Promising Strain ofTrichoderma evansii(WF-3) for Extracellularα-Galactosidase Production by Utilizing Different Carbon Sources under Optimized Culture Conditions

2014 ◽  
Vol 2014 ◽  
pp. 1-12
Author(s):  
Aishwarya Chauhan ◽  
Nikhat Jamal Siddiqi ◽  
Bechan Sharma
Keyword(s):  
Enzyme Activity ◽  
Wheat Straw ◽  
Guar Gum ◽  
Carbon Sources ◽  
Culture Conditions ◽  
Enzyme Synthesis ◽  
Basic Medium ◽  
Growth Media ◽  
Bean Meal ◽  
Soya Bean Meal

A potential fungal strain ofTrichodermasp. (WF-3) was isolated and selected for the production ofα-galactosidase. Optimum conditions for mycelial growth and enzyme induction were determined. Basal media selected for the growth of fungal isolate containing different carbon sources like guar gum (GG), soya bean meal (SM), and wheat straw (WS) and combinations of these carbon substrates with basic sugars like galactose and sucrose were used to monitor their effects onα-galactosidase production. The results of this study indicated that galactose and sucrose enhanced the enzyme activity in guar gum (GG) and wheat straw (WS). Maximumα-galactosidase production (213.63 UmL−1) was obtained when the basic medium containing GG is supplemented with galactose (5 mg/mL). However, the presence of galactose and sucrose alone in the growth media shows no effect. Soya meal alone was able to supportT. evansiito produce maximum enzyme activity (170.36 UmL−1). The incubation time, temperature, and pH for the maximum enzyme synthesis were found to be 120 h (5 days), 28°C, and 4.5–5.5, respectively. All the carbon sources tested exhibited maximum enzyme production at 10 mg/mL concentration. Among the metal ions tested, Hg was found to be the strongest inhibitor of the enzyme. Among the chelators, EDTA acted as stronger inhibitor than succinic acid.

scholarly journals Optimization of Cultural Conditions for Production of Antibacterial Metabolites from Streptomyces coelicoflavus BC 01

2015 ◽  
Vol 7 (2) ◽  
pp. 151-159
Author(s):  
Kothagorla Venkata RAGHAVA RAO ◽  
Dadi BHASKARA RAO ◽  
Botcha SATYANARAYANA ◽  
Tamanam RAGHAVA RAO
Keyword(s):  
Andhra Pradesh ◽  
Maximum Yield ◽  
Nitrogen Sources ◽  
Fermentation Medium ◽  
Carbon And Nitrogen ◽  
Mangrove Soil ◽  
Cultural Conditions ◽  
Bean Meal ◽  
Metabolites Production ◽  
Soya Bean Meal

The aim of the present study was to optimize various cultural conditions for the production of antibacterial metabolites by Streptomyces coelicoflavus BC 01 isolated from mangrove soil, Visakhapatnam, Andhra Pradesh, India. The effect of various factors such as carbon and nitrogen sources, different concentrations of NaCl and K2HPO4, different temperature, pH, incubation time and agitation on antibacterial metabolites production were studied. The production of antibacterial metabolites by the isolate Streptomyces coelicoflavus BC 01 was greatly influenced by the cultural conditions. Glucose (1.2%) and soya bean meal (1%) seemed to be the best carbon and nitrogen source respectively, followed by NaCl (1%) and K2HPO4 (0.25%). Maximum production of antibacterial metabolites was observed at a temperature of 30 °C, with pH 7.2, at 160 rpm for 96 hrs. These optimized parameters can be further useful to design a fermentation medium to achieve maximum yield of antibacterial metabolites from Streptomyces coelicoflavus BC 01.

A comparison of sources of supplementary nitrogen for young cattle receiving fibre-rich diets

1980 ◽  
Vol 95 (3) ◽  
pp. 687-695 ◽  
Author(s):  
T. Smith ◽  
Valerie J. Broster ◽  
R. E. Hill
Keyword(s):  
Nitrogen Retention ◽  
Live Weight ◽  
Nitrogen Sources ◽  
Soya Bean ◽  
Nitrogen Concentrations ◽  
Feeding Trials ◽  
Bean Meal ◽  
Young Cattle ◽  
Soya Bean Meal ◽  
Protein Supply

SUMMARYFishmeal, soya-bean meal and urea were compared as nitrogen sources in diets rich in fibre for yearling cattle, using feeding trials and digestibility and nitrogen retention studies. All animals were individually fed. Diets supplemented with fishmeal supported the highest rates of daily live-weight gain and nitrogen retention. There was no response in dry-matter intake and digestibility from extra nitrogen, either from fishmeal or urea, when the crude protein of the diet was 8·5% or over, and a small response in digestibility when soya-bean meal was used.Molar proportions of VFA, rumen NH3-N concentrations and blood urea nitrogen concentrations were all affected by both amount and source of nitrogen supplementation. Multiple regression analysis showed the undegradable protein supply to be more critical with high than with low fibre diets.

scholarly journals Effect of Cultural Conditions on Protease Production by a Thermophilic Geobacillus thermoglucosidasius SKF4 Isolated from Sungai Klah Hot Spring Park, Malaysia

Molecules ◽  
2020 ◽  
Vol 25 (11) ◽  
pp. 2609
Author(s):  
Allison D. Suleiman ◽  
Nor’Aini Abdul Rahman ◽  
Hidayat Mohd Yusof ◽  
Fairolniza Mohd Shariff ◽  
Nur Adeela Yasid
Keyword(s):  
Incubation Time ◽  
Hot Spring ◽  
Nitrogen Sources ◽  
Protease Production ◽  
Rrna Gene ◽  
16S Rrna Gene Analysis ◽  
Geobacillus Thermoglucosidasius ◽  
Cultural Conditions ◽  
Protease Enzyme ◽  
Nacl Concentration

Major progress in the fields of agriculture, industry, and biotechnology over the years has influenced the quest for a potent microorganism with favorable properties to be used in scientific research and industry. This study intended to isolate a new thermophilic-protease-producing bacterium and evaluate its growth and protease production under cultural conditions. Protease producing bacteria were successfully isolated from Sungai Klah Hot Spring Park in Perak, Malaysia, and coded as SKF4; they were promising protease producers. Based on microscopic, morphological, and 16S rRNA gene analysis, isolate SKF4 was identified as Geobacillus thermoglucosidasius SKF4. The process of isolating SKF4 to grow and produce proteases under different cultural conditions, including temperature, pH, NaCl concentration, carbon and nitrogen sources, and incubation time, was explored. The optimum cultural conditions observed for growth and protease production were at 60 to 65 °C of temperature, pH 7 to 8, and under 1% NaCl concentration. Further, the use of casein and yeast extract as the nitrogen sources, and sucrose and fructose as the carbon sources enhanced the growth and protease production of isolate SKF4. Meanwhile, isolate SKF4 reached maximum growth and protease production at 24 h of incubation time. The results of this study revealed a new potent strain of thermophilic bacterium isolated from Sungai Klah Hot Spring Park in Perak, Malaysia for the first time. The high production of thermostable protease enzyme by G. thermoglucosidasius SKF4 highlighted the promising properties of this bacterium for industrial and biotechnological applications.

scholarly journals Optimum vegetative growth conditions and genetic diversity in different strains of Pleurotus salmoneastramineus L.J.N. Vassilzeva

2020 ◽  
Vol 49 (1) ◽  
pp. 125-134
Author(s):  
Nuhu Alam ◽  
Farhana Rahman
Keyword(s):  
Genetic Diversity ◽  
Mycelial Growth ◽  
Culture Media ◽  
Inorganic Nitrogen ◽  
Carbon Sources ◽  
Nitrogen Sources ◽  
Its Region ◽  
Growth Conditions ◽  
Malt Extract ◽  
Arbitrary Primers

This experiment was undertaken to depict the favourable condition for mycelial growth, molecular identification and phylogenetic relationship of the selected strains of Pleurotus salmoneostramineus. Suitable temperature and pH were obtained at 25ºC and 6, respectively. Mushroom complete, glucose peptone and yeast malt extract culture media were favorable, while Hennerberg and Hoppkins were unfavorable. Dextrin was the best and xylose was the less effective carbon sources. Inorganic nitrogen sources were less effective for the mycelial growth of P. salmoneostramineus. The sequences of internal transcribed spacer (ITS) region of selected strains revealed that the total length ranged from 614 to 663 bp. The size of the ITS1 and ITS2 regions varied among the strains. Sequence analysis showed that 5.8S of rDNA sequences were identical. Phylogenetic tree of the ITS region sequences indicated that strains of P. salmoneostramineus belong to same cluster. The reciprocal homologies of the ITS region sequences ranged from 98 to 100%. The strains of P. salmoneostramineus were also analyzed by random amplification of polymorphic DNA (RAPD) with 20 arbitrary primers. RAPD results suggested that tested strains of P. salmoneostramineus were genetically similar with some variations, thus it could be concluded that RAPD and ITS techniques were well competent for detecting the genetic diversity of all tested strains of P. salmoneostramineus.

scholarly journals Random mutagenesis and process optimization of bacterial co-culture for hyperproduction of 1,4-α-D-glucan glucanohydrolase using submerged fermentation

2018 ◽  
Vol 72 (6) ◽  
pp. 329-339
Author(s):  
Roheena Abdullah ◽  
Samra Kiran ◽  
Mehwish Iqtedar ◽  
Afshan Kaleem ◽  
Faiza Saleem ◽  
...  
Keyword(s):  
Uv Light ◽  
Carbon Sources ◽  
Random Mutagenesis ◽  
Strain Improvement ◽  
Nitrogen Sources ◽  
Tween 80 ◽  
Fold Increase ◽  
Inoculum Size ◽  
Medium Composition ◽  
Chemical Mutagenesis

The exponential increase in the application of 1,4-?-D-glucan glucanohydrolase (GGH) in various fields has placed stress and demand in both qualitative improvement and quantitative enhancement through strain improvement. In the present work, Bacillus subtilis LCBT-15 and Bacillus amyloliquefaciens LCBT-20 were subjected to physical as well as chemical mutagenesis for improving the GGH production potential. Applications of the UV light and ethidium bromide did not cause a significant increase in the enzyme production. However, Ethyl methane sulphonate (EMS) treated co-culture 10 gave 1.3-fold increase in the GGH production, in contrast to the wild co-culture. Different physicochemical parameters including fermentation media, rate of fermentation, temperature, pH, nitrogen and carbon sources and surfactants were also investigated. The M7 medium composition was optimized for GGH production after 48h of incubation at 37?C and pH 6. The optimum inoculum size was 3.5 ml (1x106 cells/ml) in 50 ml of medium. The best carbon and nitrogen sources were lactose (2.5 %); ammonium chloride (1.75 %) and beef extract (1 %), respectively. Optimal GGH production (287 U/ml) was obtained when the medium was supplemented with 0.05% Tween 80. The novelty of this work was exploration of the synergistic phenomena of mutant bacterial co-culture for the enhancement of GGH production.

Cytotoxic Effect of Pseudomonas aurogenosa Protease on Cancer Cells

2019 ◽  
Vol 10 (03) ◽  
Author(s):  
Ghanyia J. Shanyoor ◽  
Fatima R. Abdul ◽  
Nehad A. Taher ◽  
Ihsan A. Raheem
Keyword(s):  
Cell Line ◽  
Normal Cell ◽  
Cytotoxic Effect ◽  
Human Cancer ◽  
Skim Milk ◽  
Exchange Chromatography ◽  
Protease Production ◽  
Normal Cell Line ◽  
Protease Enzyme ◽  
Embryonic Fibroblast

About (20) Pseudomonas rogenosa isolate were experienced for their ability of protease production by calculating the diameter of lysis area after developing on skim milk agar medium (qualitatively ) and the results exhibited that only isolate no (5), was higher isolate for protease making of (26mm) of lysis area. Then, the protein concentration also identified by Bradford method and it was found of 0.16 mg/ ml , then purification was done by using an ion- exchange chromatography with DEAE sephadex G- 100 column and the results showed the presence of 1 peak of enzyme with 50 Kd of molecular weight 2 peaks of other proteins . we tried to investigate the invitro Cytotoxic effect of purified enzyme against two human cancer lines, HeP2 (Human larynx epidermed carcinoma ) , RD ( Rabdo- Sarcoma ) , and one normal cell line Ref ( Rat embryonic fibroblast ) . The cancer and normal cells were treated with different concentrations of protease enzyme ranging from ( 0.05, 0.1, 0.2, 0.4,0.8and 0.16 mg/ml) then incubated for additional 48h at 37C0 and the results showed highest toxicity ( 80.28%) of protease enzyme on RD , moderate cytotoxicity (45.52%) on Hep andslight toxicity ( 37.12% ) on normal cell line (Ref) in a concentration (0.8mg/ml).

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