scholarly journals Anxiolytic and Anticonvulsant Effects on Mice of Flavonoids, Linalool, and -Tocopherol Presents in the Extract of Leaves ofCissus sicyoidesL. (Vitaceae)

2009 ◽  
Vol 2009 ◽  
pp. 1-6 ◽  
Author(s):  
Edvaldo Rodrigues de Almeida ◽  
Krissia Rayane de Oliveira Rafael ◽  
Geraldo Bosco Lindoso Couto ◽  
Ana Beatriz Matos Ishigami
Keyword(s):  
Elevated Plus Maze ◽  
Sodium Pentobarbital ◽  
Hydroalcoholic Extract ◽  
Male And Female ◽  
Aerial Parts ◽  
Plus Maze ◽  
Marble Burying ◽  
Males And Females ◽  
Behavioral Assays ◽  
Anticonvulsant Effects

The aim of the present study is to demonstrate the anxiolytic and anticonvulsant effects of a hydroalcoholic extract obtained from the aerial parts ofCissus sicyoidesL. (CS) (Vitaceae) on male and female mice using several behavioral assays. Groups of males and females treated via intraperitoneal (IP) with doses of 300, 600, and 1000 mg/kg of the extract showed significant action in the elevated plus-maze (EPM), time spent in the open arms, and number of entries in the open arms. The board-hole test also showed a significant increase in the time spent in head-dipping and in marble-burying test of the number of marbles buried. The same treatment increased the duration of sleeping time induced by sodium pentobarbital and also showed a significant increase in protection against pentylenotetrazole-induced convulsions. These results indicate an anxiolytic and anticonvulsant-like action fromC. sicyoidesL. extract on mice, probably due to the action of flavonoid(s), Linalool, and -tocopherol present in theC. sicyoidesleaves.

Neuropharmacological Characterization of Dioclea altissima Seed Lectin (DAL) in Mice: Evidence of Anxiolytic-like Effect Mediated by Serotonergic, GABAergic Receptors and NO Pathway

2020 ◽  
Vol 26 (31) ◽  
pp. 3895-3904
Author(s):  
João R.C. Araújo ◽  
Adriana R. Campos ◽  
Marina de Barros M.V. Damasceno ◽  
Sacha A.A.R. Santos ◽  
Maria K.A. Ferreira ◽  
...  
Keyword(s):  
Structural Integrity ◽  
Elevated Plus Maze ◽  
Biological Activities ◽  
Plant Lectins ◽  
Plus Maze ◽  
Marble Burying ◽  
Gabaergic Receptors ◽  
The Central Nervous System ◽  
Hole Board

Background: Plant lectins have shown promising biological activities in the central nervous system (CNS). Objective: This study evaluated the effect of DAL, a lectin isolated from the seeds of the Dioclea altissima species, having binding affinity to D-glucose or D-mannose residues, on mice behavior. Methods: Mice (n=6/group) were treated (i.p.) with DAL (0.25, 0.5 or 1 mg/kg) or vehicle and subjected to several tests (open field/OFT, marble-burying/MBT, hole-board/HBT, elevated plus maze/PMT, tail suspension/ TST, forced swimming/FST or rotarod/RRT). Pizotifen, cyproheptadine, flumazenil, L-NAME, 7-NI, Larginine or yohimbine were administered 15 min before DAL (0.5 mg/kg) and the animals were evaluated on PMT. It was also verified whether the DAL effect depended on its structural integrity and ability to interact with carbohydrates. Results: The results showed there were no neurobehavioral changes in the mice at the RRT, FST and locomotion in the OFT. DAL (0.25, 0.5 or 1 mg/kg) increased the behavior of grooming and rearing in the OFT, head dips in the HBT, pedalling in the TST and decreased the number of marbles hidden in the MBT. In the PMT, DAL (0.25, 0.5 and 1 mg/kg) and Diazepam increased the frequency of entries in the open arms and the time of permanence in the open arms without affecting the locomotor activity. The effect of DAL was dependent on carbohydrate interaction and protein structure integrity and it prevented by pizotifen, cyproheptadine, flumazenil, L-NAME and 7-NI, but not by L-arginine or yohimbine. Conclusion: DAL was found to have an anxiolytic-like effect mediated by the 5-HT and GABAergic receptors and NO pathway.

scholarly journals Involvement of 5-HT1AReceptors in the Anxiolytic-Like Effects of Quercitrin and Evidence of the Involvement of the Monoaminergic System

2016 ◽  
Vol 2016 ◽  
pp. 1-10 ◽  
Author(s):  
Jian Li ◽  
Qian-tong Liu ◽  
Yi Chen ◽  
Jie Liu ◽  
Jin-li Shi ◽  
...  
Keyword(s):  
Receptor Antagonist ◽  
Elevated Plus Maze ◽  
Experimental Models ◽  
Control Group ◽  
Repeated Treatment ◽  
Plus Maze ◽  
Way 100635 ◽  
Marble Burying ◽  
Monoaminergic System ◽  
Light Dark Box

Quercitrin is a well-known flavonoid that is contained in Flos Albiziae, which has been used for the treatment of anxiety. The present study investigated the anxiolytic-like effects of quercitrin in experimental models of anxiety. Compared with the control group, repeated treatment with quercitrin (5.0 and 10.0 mg/kg/day, p.o.) for seven days significantly increased the percentage of entries into and time spent on the open arms of the elevated plus maze. In the light/dark box test, quercitrin exerted an anxiolytic-like effect at 5 and 10 mg/kg. In the marble-burying test, quercitrin (5.0 and 10.0 mg/kg) also exerted an anxiolytic-like effect. Furthermore, quercitrin did not affect spontaneous locomotor activity. The anxiolytic-like effects of quercitrin in the elevated plus maze and light/dark box test were blocked by the serotonin-1A (5-hydroxytryptamine-1A (5-HT1A)) receptor antagonist WAY-100635 (3.0 mg/kg, i.p.) but not by theγ-aminobutyric acid-A (GABAA) receptor antagonist flumazenil (0.5 mg/kg, i.p.). The levels of brain monoamines (5-HT and dopamine) and their metabolites (5-hydroxy-3-indoleacetic acid, 3,4-dihydroxyphenylacetic acid, and homovanillic acid) were decreased after quercitrin treatment. These data suggest that the anxiolytic-like effects of quercitrin might be mediated by 5-HT1Areceptors but not by benzodiazepine site of GABAAreceptors. The results of the neurochemical studies suggest that these effects are mediated by modulation of the levels of monoamine neurotransmitters.

scholarly journals Central activities of hydroalcoholic extract from Lafoensia pacari A. St.-Hil. stem bark

2010 ◽  
Vol 46 (3) ◽  
pp. 455-462 ◽  
Author(s):  
Pablinny Moreira Galdino ◽  
Marcus Vinícius Mariano Nascimento ◽  
Fábio Borges de Sousa ◽  
Reginaldo Nassar Ferreira ◽  
José Realino de Paula ◽  
...  
Keyword(s):  
Elevated Plus Maze ◽  
Field Tests ◽  
Sleep Time ◽  
Stem Bark ◽  
Rio Grande Do Sul ◽  
Hydroalcoholic Extract ◽  
Sleeping Time ◽  
Plus Maze ◽  
Immobility Time ◽  
Time Test

Lafoensia pacari A. St.-Hil. can be found from Amapá to Rio Grande do Sul states, and also in Paraguay and Bolivia. It is popularly known as pacari or mangava-brava and is used to promote weight loss, as an anti-thermal or tonic, to treat gastritis, ulcers, scarring, itching, discouragement, and cancer. In the open field tests, the hydroalcoholic extract from L. pacari stem bark (HEP) decreased the number of rearings, number of invaded squares, and increased immobility time compared to control animals. In the pentobarbital-induced sleep time test, HEP decreased latency time to sleep and increased sleeping time. In the rota-rod test, no changes in the studied parameters were observed. In the elevated plus maze, HEP increased the percentage time and percentage entries in the open arms, indicating that this extract exerts an anxiolytic-like activity.

scholarly journals Mice carrying paternal knockout of imprinted Grb10 do not show compulsive behaviours

2020 ◽  
Author(s):  
Kira DA Rienecker ◽  
Alexander T Chavasse ◽  
Kim Moorwood ◽  
Andrew Ward ◽  
Trevor Humby ◽  
...  
Keyword(s):  
Risk Taking ◽  
Social Behaviour ◽  
Elevated Plus Maze ◽  
Imprinted Genes ◽  
Wild Type ◽  
Wild Type Littermate ◽  
Plus Maze ◽  
Marble Burying ◽  
Compulsive Behaviour

ABSTRACTMice lacking paternal expression of imprinted Grb10 show a number of social behaviour deficits, including an enhanced allogrooming phenotype. However, this could also index compulsive behaviour, and the increased whisker barbering seen in Grb10+/p mice has been suggested to be indicative of a trichotillomania-type behaviour. Here we test whether compulsive behaviour is a more general phenotype in Grb10+/p mice by examining marble burying at three different adult ages (2, 6 and 10 months). We also examined the mice for potentially confounding anxiety phenotypes using the elevated plus maze (EPM). Grb10+/p mice showed no difference from wild-type littermate controls on any measure in the marble burying test at any age. There was no difference in standard anxiety measures either, although Grb10+/p mice displayed more risk-taking behaviours on the EPM than wild-type mice. These data suggest that Grb10+/p mice are not generally more compulsive, and that the enhanced allogrooming is probably indicative of altered social behaviour. Furthermore, the altered behaviours seen on the EPM adds to other published findings suggesting that Grb10, and imprinted genes more generally, have a role in mediating risk-taking behaviour.

scholarly journals Dose-Dependent, Antidepressant, and Anxiolytic Effects of a Traditional Medicinal Plant for the Management of Behavioral Dysfunctions in Animal Models

Dose-Response ◽  
2019 ◽  
Vol 17 (4) ◽  
pp. 155932581989126 ◽  
Author(s):  
Hafiz Muhammad Asif ◽  
Abdul Hayee ◽  
Muhammad Rahil Aslam ◽  
Khalil Ahmad ◽  
Abdul Sattar Hashmi
Keyword(s):  
Open Field ◽  
Elevated Plus Maze ◽  
Tail Suspension Test ◽  
Antidepressant Effect ◽  
Tail Suspension ◽  
Hydroalcoholic Extract ◽  
Elevated Plus Maze Test ◽  
Plus Maze ◽  
Immobility Time ◽  
Dose Dependent

The present work was carried out to assess the Onosma bracteatum anxiolytic and antidepressant properties. Swiss albino mice (male) were fed orally with hydroalcoholic extract at different doses 50, 100, and 200 mg 1 hour prior to test with the standard diazepam and fluoxetine. Anxiolytic and antidepressant activities were evaluated by using open field, elevated plus maze, force swimming, and tail suspension test. Results of open field test showed an increase in number of line crossing as well as number of rearing in dosage-dependent design. Although results of elevated plus maze test evidently showed antianxiety effect of O bracteatum by increasing the time spent in open arms along with decreasing the time spent in closed arms in dosage-dependent way. For the evaluation of antidepressant effect, O bracteatum diminished the immobility time and expanded mobility time in forced swim model in dosage-dependent way. Likewise, O bracteatum expanded time span of mobility along with diminished immobility time in tail suspension method in dosage-dependent way. Outcome demonstrated that plant at the dose of 200 mg/kg body weight showed significant potential which was similar to that standard diazepam and fluoxetine. Hence, O bracteatum may be used as potent natural psychotherapeutic agent against the mental disorders.

Effects of repeated asenapine in a battery of tests for anxiety-like behaviours in mice

2015 ◽  
Vol 28 (2) ◽  
pp. 85-91 ◽  
Author(s):  
Hila M Ene ◽  
Nirit Z Kara ◽  
Noa Barak ◽  
Tal Reshef Ben-Mordechai ◽  
Haim Einat
Keyword(s):  
Open Field ◽  
Atypical Antipsychotic ◽  
Antipsychotic Drugs ◽  
Elevated Plus Maze ◽  
Open Field Test ◽  
Serotonergic Receptors ◽  
Serotonin System ◽  
Plus Maze ◽  
Marble Burying ◽  
Behavioural Tests

ObjectiveA number of atypical antipsychotic drugs were demonstrated to have anxiolytic effects in patients and in animal models. These effects were mostly suggested to be the consequence of the drugs’ affinity to the serotonin system and its receptors. Asenapine is a relatively new atypical antipsychotic that is prescribed for schizophrenia and for bipolar mania. Asenapine has a broad pharmacological profile with significant effects on serotonergic receptors, hence it is reasonable to expect that asenapine may have some anxiolytic effects. The present study was therefore designed to examine possible effects of asenapine on anxiety-like behaviour of mice.MethodMale ICR mice were repeatedly treated with 0.1 or 0.3 mg/kg injections of asenapine and then tested in a battery of behavioural tests related to anxiety including the open-field test, elevated plus-maze (EPM), defensive marble burying and hyponeophagia tests. In an adjunct experiment, we tested the effects of acute diazepam in the same test battery.ResultsThe results show that diazepam reduced anxiety-like behaviour in the EPM, the defensive marble burying test and the hyponeophagia test but not in the open field. Asenapine has anxiolytic-like effects in the EPM and the defensive marble burying tests but had no effects in the open-field or the hyponeophagia tests. Asenapine had no effects on locomotor activity.ConclusionThe results suggest that asenapine may have anxiolytic-like properties and recommends that clinical trials examining such effects should be performed.

Dietary Soy Supplements Produce Opposite Effects on Anxiety in Intact Male and Female Rats in the Elevated Plus-Maze.

2005 ◽  
Vol 119 (2) ◽  
pp. 587-594 ◽  
Author(s):  
Heather B. Patisaul ◽  
Adele Blum ◽  
Jordan R. Luskin ◽  
Mark E. Wilson
Keyword(s):  
Elevated Plus Maze ◽  
Female Rats ◽  
Intact Male ◽  
Male And Female ◽  
Male And Female Rats ◽  
Plus Maze

scholarly journals Study of oral aniracetam in C57BL/6J mice without pre-existing cognitive impairments

F1000Research ◽  
2017 ◽  
Vol 6 ◽  
pp. 1452
Author(s):  
Conner D. Reynolds ◽  
Taylor S. Jefferson ◽  
Meagan Volquardsen ◽  
Ashvini Pandian ◽  
Gregory D. Smith ◽  
...  
Keyword(s):  
Cognitive Dysfunction ◽  
Healthy Subjects ◽  
Elevated Plus Maze ◽  
Cognitive Impairments ◽  
Therapeutic Benefit ◽  
Novel Object Recognition ◽  
Novel Object ◽  
Plus Maze ◽  
Marble Burying ◽  
Enhanced Learning

Background: The piracetam analog, aniracetam, has recently received attention for its cognition enhancing potential, with minimal reported side effects.  Previous studies report the drug to be effective in both human and non-human models with pre-existing cognitive dysfunction, but few studies have evaluated its efficacy in healthy subjects. A previous study performed in our laboratory found no cognitive enhancing effects of oral aniracetam administration 1-hour prior to behavioral testing in naïve C57BL/6J mice. Methods: The current study aims to further evaluate this drug by administration of aniracetam 30 minutes prior to testing in order to optimize any cognitive enhancing effects. In this study, all naïve C57BL/6J mice were tested in tasks of delayed fear conditioning, novel object recognition, rotarod, open field, elevated plus maze, and marble burying. Results: Across all tasks, animals in the treatment group failed to show enhanced learning when compared to controls. Conclusions: These results provide further evidence suggesting that aniracetam conveys no therapeutic benefit to subjects without pre-existing cognitive dysfunction.

scholarly journals Oxycodone decreases anxiety-like behavior in the elevated plus-maze test in male and female rats

2021 ◽  
Author(s):  
Adriaan W. Bruijnzeel ◽  
Azin Behnood-Rod ◽  
Wendi Malphurs ◽  
Ranjithkumar Chellian ◽  
Robert M. Caudle ◽  
...  
Keyword(s):  
Locomotor Activity ◽  
Field Test ◽  
Elevated Plus Maze ◽  
Open Field Test ◽  
Female Rats ◽  
Male And Female ◽  
Elevated Plus Maze Test ◽  
Maze Test ◽  
Male And Female Rats ◽  
Plus Maze

AbstractThe prescription opioid oxycodone is widely used for the treatment of pain in humans. Oxycodone misuse is more common among people with an anxiety disorder than those without one. Therefore, oxycodone might be misused for its anxiolytic properties. We investigated if oxycodone affects anxiety-like behavior in adult male and female rats. The rats were treated with oxycodone (0.178, 0.32, 0.56, or 1 mg/kg), and anxiety-like behavior was investigated in the elevated plus-maze test. Immediately after the elevated plus-maze test, a small open field test was conducted to determine the effects of oxycodone on locomotor activity. In the elevated plus-maze test, oxycodone increased the percentage of time spent on the open arms, the percentage of open arm entries, time on the open arms, open arm entries, and the distance traveled. The males treated with vehicle had a lower percentage of open arm entries than the females treated with vehicle, and oxycodone treatment led to a greater increase in the percentage of open arm entries in the males than females. Furthermore, the females spent more time on the open arms, made more open arm entries, spent less time in the closed arms, and traveled a greater distance than the males. In the small open field test, treatment with oxycodone did not affect locomotor activity or rearing. Sex differences were observed; the females traveled a greater distance and displayed more rearing than the males. In conclusion, oxycodone decreases anxiety-like behavior in rats, and oxycodone has a greater anxiolytic-like effect in males than females.

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