scholarly journals Changes in Bacterial Population of Gastrointestinal Tract of Weaned Pigs Fed with Different Additives

2014 ◽  
Vol 2014 ◽  
pp. 1-13 ◽  
Author(s):  
Mercè Roca ◽  
Miquel Nofrarías ◽  
Natàlia Majó ◽  
Ana María Pérez de Rozas ◽  
Joaquim Segalés ◽  
...  

This study aimed to provide novel insights into the gastrointestinal microbial diversity from different gastrointestinal locations in weaning piglets using PCR-restriction fragment length polymorphism (PCR-RFLP). Additionally, the effect of different feed additives was analyzed. Thirty-two piglets were fed with four different diets: a control group and three enriched diets, with avilamycin, sodium butyrate, and a plant extract mixture. Digesta samples were collected from eight different gastrointestinal segments of each animal and the bacterial population was analysed by a PCR-RFLP technique that uses 16S rDNA gene sequences. Bacterial diversity was assessed by calculating the number of bands and the Shannon-Weaver index. Dendrograms were constructed to estimate the similarity of bacterial populations. A higher bacterial diversity was detected in large intestine compared to small intestine. Among diets, the most relevant microbial diversity differences were found between sodium butyrate and plant extract mixture. Proximal jejunum, ileum, and proximal colon were identified as those segments that could be representative of microbial diversity in pig gut. Results indicate that PCR-RFLP technique allowed detecting modifications on the gastrointestinal microbial ecology in pigs fed with different additives, such as increased biodiversity by sodium butyrate in feed.

2017 ◽  
Vol 1 (2) ◽  
pp. 186-200 ◽  
Author(s):  
E. Barba-Vidal ◽  
V. F. B. Roll ◽  
L. Castillejos ◽  
A. A. Guerra-Ordaz ◽  
X. Manteca ◽  
...  

Abstract Salmonella spp. is one of the worldwide leading causes of food-borne illnesses for which the inclusion of probiotics or organic acids in animal feeds can be useful control methods. Experimental models are utilized to test the efficacy of strategies against pathogens, but they exhibit limitations which may preclude finding sensible evaluation parameters. The objective of this work is to evaluate the efficacy of 2 different feed additives; a Bacillus licheniformis based probiotic and a protected sodium butyrate (SB) salt, using an experimental model of salmonellosis and, second, to explore if behavior analysis can be used as a sensible evaluation tool for additives evaluation. A total of 78 piglets weaned at 24 d, 8.3 kg BW, were used. Seventy-two were placed in 3 rooms of 8 pens (3 animals/pen) with evenly distributed treatments (n = 8): CON, control group with plain diet; PRO, plain diet with 1 kg/t of Proporc (109 cfu of B. licheniformis/kg of feed), and BUT, plain diet with 3 kg/t of Gustor BP70 (2.1 g of partially protected SB salt/kg of feed). Remaining piglets (n = 6) were separated and used as a challenge negative control. The experiment lasted 16 d. After 1 wk of adaptation, animals were challenged with 5 × 108 cfu of Salmonella Typhimurium. One pig per pen was euthanized and sampled at d 4 and 8 post-inoculation (PI). There were no significant differences among treatments for ADFI, ADG, G:F, rectal temperature, fecal consistency, pH, ammonia, short-chain fatty acids and lactic acid concentrations, cytokine TNF-α, Pig-MAP acute-phase proteins and histological parameters. However, both products were equally able to reduce colonization and shedding of Salmonella (P = 0.016 for PRO and BUT vs. CON). In addition, PRO treatment had a positive effect on behavioral displays, particularly exploring (P < 0.05 vs. CON), feeding (P < 0.05 vs. CON and BUT) and other active behaviors (P < 0.05 vs. CON and BUT) in the morning period (0830 to 1030 h). In the afternoon (1400 to 1600 h), the challenge effect was most significant. Pigs were less active after the challenge (P < 0.001), with a decrease in positive contacts (P = 0.004), exploration (P < 0.001) and feeding behaviors (P < 0.001) on d 3 PI, in comparison with before the challenge. Accordingly, many lying conducts increased at d 3 PI (P < 0.05). In conclusion, both treatments had positive effects against Salmonella, and behavior analysis appears to be a sensible tool to be considered.


Author(s):  
M. Grigoryev ◽  
N. Chernogradskaya

Animals imported to Yakutia from other regions fall into feeding and housing conditions that differ from their usual agro-climatic conditions, which causes the problem of their adaptation. Yakutia is one of the areas where the feed of its own production has a lack of basic minerals, which affects the health and productivity of animals, which has an additional negative influence on the adaptation process of imported cattle. In order to replenish macro- and microelements in the body, it is necessary to introduce various local mineral feed additives, premixes, and use mineral salts into the ration. Under the conditions of Central Yakutia the influence of local mineral feed additives (zeolite of the Khonguruu Deposit in the Suntar district and sapropels of local lakes) containing macro- and microelements that increase the meat productivity of steers has been studied. As a result of the research the influence of local mineral feed additives on meat productivity and physiological condition of Hereford steers has been established. Influence of zeolite and sapropel in a complex with mineral salts on digestibility of nutrients of the ration and use of nitrogen, calcium and phosphorus by experimental animals has been defined. The influence of feed additives on the quality of meat products has been studied. The results have shown that the use of local mineral feed additives in the composition of ration of steers during the fattening period at a dose of 0,7 g zeolite per 1 kg of live weight of the animal and 200 g of sapropel in combination with 10 g of mineral salts contributed to the gain in live weight, carcass weight, carcass yield, yield of edible parts, and economic efficiency of fattening. For the entire period of the experiment the gain in live weight in groups was: in control group 2222,9 kg, in the 1st experimental group 2396,2 kg, in the 2nd experimental group 2471,1 kg. The total economic effect for the 1st experimental group for the period of experiment amounted to 44,3 thousand Rubles, for the 2nd experimental group 78,0 thousand Rubles or per 1 head/day 14,06 and 24,76 Rubles, respectively.


Author(s):  
S. Grikshas ◽  
N. Kulmakova ◽  
K. Spitsyna ◽  
A. Dar’in ◽  
T. Mittelshtein

Mycotoxins have been formed in feed are secondary metabolites of fungi and are quite stable substances that have teratogenic, mutagenic and carcinogenic effects. An effective way to combat mycotoxins in feed is the use of feed additives that adsorb toxins, prevent their absorption in the gastrointestinal tract of the animal and are excreted from the body. The infl uence of coconut enterosorbent Shelltic Es on fattening and meat qualities of young pigs has been studied. It has been found in the process of fattening that the highest feed digestibility was in pigs of the experimental group, in the diet of which enterosorbent has been added. The results of studies of the chemical composition and technological properties of pork have been provided. The positive eff ect of enterosorbent on precocity, absolute average daily gain of live weight and reduction of feed expenditures per 1 kg of gain has been revealed. In pigs from the experimental group the average thickness of the fat was higher and the area of the “muscle eye” was lower compared with animals of the control group, which indicates that higher rates of carcass yield have been obtained due to faster accumulation of fat tissue. The weight of internal organs of pigs indicates the intensity of metabolic processes in the body. In experimental animals the weight of the lungs was 0,1 kg lower, and the liver and heart were higher by 0,13 and 0,01 kg, respectively. Enterosorbent had no effect on the content of vitamins in the liver of pigs of the compared groups. The content of impurities of organochlorine toxicants and toxic elements in the meat and liver of animals of the experimental group was lower than that of control analogues. Therefore, the use of enterosorbent Shelltic Es promotes more active excretion of them from the body.


Author(s):  
S. Sukhanova ◽  
N. Pozdnyakova ◽  
F. Yaroslavtsev

The results of researches on the development of new mineral feed additives based on raw materials produced by Uralchem and the study of the eff ectiveness of their use in the rations of lactating cows have been presented in the article. The experiment has been carried out in the conditions of LLC “PF Barabinskoe” in the Dalmatovsky area in the Kurgan region where 3 groups of lactating cows of Black-and-White breed have been formed. Animals in the control group have been received the ration adopted in the farm, the 1st experimental group with the additive RusMA No. 1 at a dose of 150 g, the 2nd experimental group– with the additive RusMA No. 2 at the dose of 350 g. The use of experimental mineral additives in feeding lactating cows allowed us to increase their milk productivity and profi tability of production. At the same time, the best eff ectiveness was shown by the additive RusMA No. 2 at the dose of 350 g/head/day. The use of RusMA additives during the experiment period (105 days) allowed to increase the milk productivity of cows in the 1st experimental group by 6,03 % and in the 2nd experimental group by 8,24 %. Taking into account the fat content in milk, this diff erence was 6,31 and 8,53 %, respectively. The energy value of milk from cows of the experimental groups was higher compared to the control group by 2,45 and 1,76 %, respectively. According to the content of dry matter in milk, cows of the 2nd experimental group exceeded the control and 1st experimental group by 0,39 and 0,12 abs.%, respectively. The maximum milk fat content has been observed in the milk of cows of the 1st experimental group by 0,03 and 0,01 abs.% more than in the control and 2nd experimental groups, respectively. In terms of protein content, the cows of the 2nd experimental group signifi cantly exceeded the control group by 0,10 abs.% and the 1st experimental by 0,04 abs.%. The content of milk sugar in the 2nd experimental group was higher than in the control by 0,30 abs.% and compared to the 1st experimental by 0,11 %. Signifi cantly more calcium and phosphorus were contained in the milk of cows of the 2nd experimental group by 4,58 % (Р ≤ 0,001) and 2,86 % (Р ≤ 0,05), compared with the control group. The expenditures of feed in the EFU for the production of 1 kg of milk in animals of the 2nd experimental group was less by 5,13 % than in control cows and by 2,63 % compared to the 1st experimental group. The prime cost of 1 centner of milk was lower in the 2nd experimental group by 3,94 %, in the 1st experimental group by 2,65 % compared to the control group. The level of profi tability of milk production in the 2nd experimental group was higher by 9,0 and 1,0 abs.% in comparison with the control and 1st experimental groups, respectively.


Author(s):  
M.T. SABITOV ◽  
A.R. FARKHUTDINOVA ◽  
M.G. MALIKOVA ◽  
N.G. FENCHENKO ◽  
N.I. KHAIRULLINA ◽  
...  

Изучена целесообразность использования в рационах телят черно-пестрой породы комплексной минерально-витаминной кормовой добавки (КМВКД). Установлено пониженное содержание в кормах таких элементов, как кальций, фосфор, магний, сера, марганец, медь, цинк и кобальт. Соотношение питательных и минеральных веществ, степень их доступности для усвоения организмом не обеспечивают их потребность. Использование КМВКД способствовало улучшению всех гематологических показателей. Все испытываемые рецепты, имеющие в своем составе разные варианты макро-, микроэлементов и витаминов положительно влияют на обменные про- цессы в организме животных. У молодняка опытной группы, получавшей подкормку по рецепту 1, количество эритроцитов в сравнении с животными контрольной группы увеличилось на 6,6, гемоглобина на 6,93 соответственно, по рецепту 2 11,05 и 11,39 по рецепту 3 16,75 и 15,27, то есть соотношение макро- и микроэлементов, имеющихся в составе КМВКД и взаимодействие между собой меди, железа и кобальта сыграли особую роль на концентрацию форменных элементов. У молодняка, получавшего подкормку по рецепту 1 количество общего белка в сравнении с животными контрольной группы увеличилось на 1,99, кальция на 16,02, каротина на 10,92 соответственно, по рецепту 2 4,91, 25,96 и 19,12 по рецепту 3 на 3,58, 27,07 и 20,21. Значительное повышение содержания белка, кальция, фосфора и каротина по сравнению с животными контрольной группы объясняется тем, что недостаток их в рационах восполнялся за счет используемой подкормки, в результате их содержание увеличилось в сыворотке, но не выходило за пределы нормативных данных и соответствовало физиологическим нормам.Practicability of using a complex mineral and vitamin feed additive (CMVFA) in the ration of black-and-white calf breed was studied. Decreased content of such elements as calcium, phosphorus, magnesium, sulfur, manganese, copper, zinc, and cobalt was registered. The correlation of nutritive and mineral substances, and the level of their availability for digestion did not provide for their need. CMVFA use facilitated the increase of all hematologic parameters. Tested recipes which had different content of sapropel and zeolite, as well as various types of macronutrients, microelements, and vitamins, positively influenced the digestive processes in animal bodies. Young stock from the test group that was receiving food additives according to recipe No. 1 showed 6.6 increase in the quantity of red blood cells compared to control group animals, and 6.93 increase in hemoglobin quantity the ones who were fed according to recipe No. 2 demonstrated the increase by 11.05 and 11.39 recipe No. 3 showed 16.75 and 15.27 increase, which means that the ratio of macronutrients and microelements present in CMVFA and the mutual interaction of copper, iron, and cobalt played a special role in the concentration of formed elements due to the fact that they are integral elements of blood. Total protein of young stock that was receiving food additives according to recipe No. 1 was 1.99 higher than in test group animals calcium 16.02 higher carotene 10.92 as for recipe No. 2 the values were increased by 4.91, 25.96, and 19.12 recipe No. 3 3.58, 27.07, and 20.21. Substantial increase in the content of protein, calcium, phosphorus, and carotene compared to control group animals can be explained by the fact that their insufficiency in animal feed was replenished due to the use of feed additives, and as a result their content increased in the serum but did not go beyond the norm and corresponded to physiological norms.


Cancers ◽  
2021 ◽  
Vol 13 (3) ◽  
pp. 529
Author(s):  
Silvia Selene Moreno-Guerrero ◽  
Arturo Ramírez-Pacheco ◽  
Luz María Rocha-Ramírez ◽  
Gabriela Hernández-Pliego ◽  
Pilar Eguía-Aguilar ◽  
...  

There is evidence that high circulating levels of IL-6 and IL-8 are markers of a poor prognosis in various types of cancer, including NB. The participation of these cytokines in the tumor microenvironment has been described to promote progression and metastasis. Our objective was to evaluate the prognostic role of genetic polymorphisms and serum levels of IL-6 and IL-8 in a cohort of Mexican pediatric patients with NB. The detection of the SNPs rs1800795 IL-6 and rs4073 and rs2227306 IL-8 was carried out by PCR-RFLP and the levels of cytokines were determined by the ELISA method. We found elevated circulating levels of IL-8 and IL-6 in NB patients compared to the control group. The genotype frequencies of the rs1800795 IL-6 and rs4073 IL-8 variants were different between the patients with NB and the control group. Likewise, the survival analysis showed that the GG genotypes of rs1800795 IL-6 (p = 0.014) and AA genotypes of rs4073 IL-8 (p = 0.002), as well as high levels of IL-6 (p = 0.009) and IL-8 (p = 0.046), were associated with lower overall survival. We confirmed the impact on an adverse prognosis in a multivariate model. This study suggests that the SNPs rs1800795 IL-6 and rs4073 IL-8 and their serum levels could be promising biomarkers of a poor prognosis, associated with overall survival, metastasis, and a high risk in Mexican children with NB.


Trials ◽  
2021 ◽  
Vol 22 (1) ◽  
Author(s):  
Ayla S. Turan ◽  
◽  
Leon M. G. Moons ◽  
Ramon-Michel Schreuder ◽  
Erik J. Schoon ◽  
...  

Abstract Background Endoscopic mucosal resection (EMR) for large colorectal polyps is in most cases the preferred treatment to prevent progression to colorectal carcinoma. The most common complication after EMR is delayed bleeding, occurring in 7% overall and in approximately 10% of polyps ≥ 2 cm in the proximal colon. Previous research has suggested that prophylactic clipping of the mucosal defect after EMR may reduce the incidence of delayed bleeding in polyps with a high bleeding risk. Methods The CLIPPER trial is a multicenter, parallel-group, single blinded, randomized controlled superiority study. A total of 356 patients undergoing EMR for large (≥ 2 cm) non-pedunculated polyps in the proximal colon will be included and randomized to the clip group or the control group. Prophylactic clipping will be performed in the intervention group to close the resection defect after the EMR with a distance of < 1 cm between the clips. Primary outcome is delayed bleeding within 30 days after EMR. Secondary outcomes are recurrent or residual polyps and clip artifacts during surveillance colonoscopy after 6 months, as well as cost-effectiveness of prophylactic clipping and severity of delayed bleeding. Discussion The CLIPPER trial is a pragmatic study performed in the Netherlands and is powered to determine the real-time efficacy and cost-effectiveness of prophylactic clipping after EMR of proximal colon polyps ≥ 2 cm in the Netherlands. This study will also generate new data on the achievability of complete closure and the effects of clip placement on scar surveillance after EMR, in order to further promote the debate on the role of prophylactic clipping in everyday clinical practice. Trial registration ClinicalTrials.gov NCT03309683. Registered on 13 October 2017. Start recruitment: 05 March 2018. Planned completion of recruitment: 31 August 2021.


2021 ◽  
Author(s):  
Florian Caillon ◽  
Katharina Besemer ◽  
Peter Peduzzi ◽  
Jakob Schelker

AbstractFlood events are now recognized as potentially important occasions for the transfer of soil microbes to stream ecosystems. Yet, little is known about these “dynamic pulses of microbial life” for stream bacterial community composition (BCC) and diversity. In this study, we explored the potential alteration of stream BCC by soil inoculation during high flow events in six pre-alpine first order streams and the larger Oberer Seebach. During 1 year, we compared variations of BCC in soil water, stream water and in benthic biofilms at different flow conditions (low to intermediate flows versus high flow). Bacterial diversity was lowest in biofilms, followed by soils and highest in headwater streams and the Oberer Seebach. In headwater streams, bacterial diversity was significantly higher during high flow, as compared to low flow (Shannon diversity: 7.6 versus 7.9 at low versus high flow, respectively, p < 0.001). Approximately 70% of the bacterial operational taxonomic units (OTUs) from streams and stream biofilms were the same as in soil water, while in the latter one third of the OTUs were specific to high flow conditions. These soil high-flow OTUs were also found in streams and biofilms at other times of the year. These results demonstrate the relevance of floods in generating short and reoccurring inoculation events for flowing waters. Moreover, they show that soil microbial inoculation during high flow enhances microbial diversity and shapes fluvial BCC even during low flow. Hence, soil microbial inoculation during floods could act as a previously overlooked driver of microbial diversity in headwater streams.


Author(s):  
Ewa Ziółkowska ◽  
Joanna Bogucka ◽  
Jan Mazurkiewicz ◽  
Mateusz Rawski ◽  
Szymon Różański ◽  
...  

AbstractCommon carp (Cyprinus carpio L.) is a dominant fish species in aquaculture, and as it is a stomachless species, absorption and digestion of nutrients take place in the intestine. The aim of the study was to evaluate the effects of a prebiotic on the content of selected minerals found in the meat, gills, and skeleton of common carp. The research applied trans-galactooligosaccharide (GOS) prebiotic produced by enzymatic transgalactosylation of milk lactose by whole cells of Bifidobacterium bifidum. The following diets have been applied: control diet without feed additives (C), diet 2 (B1) with 1% of GOS, and diet 3 (B2) with 2% of GOS. In the freeze-dried samples, concentrations of the analyzed metals were determined using atomic absorption spectroscopy (AAS). The content of phosphorus was determined using colorimetric method. The analyses confirmed that the highest level of Mg was detected in the skeleton of fish fed with 1% GOS (2.51 g kg−1) and was significantly higher compared the control treatment (2.11 g kg−1) (P < 0.05). Zn content in fish meat fed with 1% GOS (35.41 mg kg−1) was significantly higher (P < 0.05) than in the control group (24.59 mg kg−1). The tissue that accumulated the greatest amount of Zn was the gills. GOS had a positive effect on Fe accumulation in the meat, gills, and skeleton. It has been concluded that supplementation of feed with 2% GOS significantly influenced the positive correlations between Mg and P in the meat and skeleton, Fe–Ca correlation in gills, and Fe–Zn correlation in the skeleton.


2007 ◽  
Vol 54 (3) ◽  
pp. 452-459 ◽  
Author(s):  
Abhrajyoti Ghosh ◽  
Bhaswar Maity ◽  
Krishanu Chakrabarti ◽  
Dhrubajyoti Chattopadhyay

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