scholarly journals Effects of a Trans-Galactooligosaccharide on Minerals Content of Common Carp (Cyprinus carpio L.) Tissues

2021 ◽  
Author(s):  
Ewa Ziółkowska ◽  
Joanna Bogucka ◽  
Jan Mazurkiewicz ◽  
Mateusz Rawski ◽  
Szymon Różański ◽  
...  
Keyword(s):  
Common Carp ◽  
Cyprinus Carpio ◽  
Control Diet ◽  
Colorimetric Method ◽  
Bifidobacterium Bifidum ◽  
Feed Additives ◽  
Control Treatment ◽  
Control Group ◽  
Whole Cells ◽  
Freeze Dried

AbstractCommon carp (Cyprinus carpio L.) is a dominant fish species in aquaculture, and as it is a stomachless species, absorption and digestion of nutrients take place in the intestine. The aim of the study was to evaluate the effects of a prebiotic on the content of selected minerals found in the meat, gills, and skeleton of common carp. The research applied trans-galactooligosaccharide (GOS) prebiotic produced by enzymatic transgalactosylation of milk lactose by whole cells of Bifidobacterium bifidum. The following diets have been applied: control diet without feed additives (C), diet 2 (B1) with 1% of GOS, and diet 3 (B2) with 2% of GOS. In the freeze-dried samples, concentrations of the analyzed metals were determined using atomic absorption spectroscopy (AAS). The content of phosphorus was determined using colorimetric method. The analyses confirmed that the highest level of Mg was detected in the skeleton of fish fed with 1% GOS (2.51 g kg−1) and was significantly higher compared the control treatment (2.11 g kg−1) (P < 0.05). Zn content in fish meat fed with 1% GOS (35.41 mg kg−1) was significantly higher (P < 0.05) than in the control group (24.59 mg kg−1). The tissue that accumulated the greatest amount of Zn was the gills. GOS had a positive effect on Fe accumulation in the meat, gills, and skeleton. It has been concluded that supplementation of feed with 2% GOS significantly influenced the positive correlations between Mg and P in the meat and skeleton, Fe–Ca correlation in gills, and Fe–Zn correlation in the skeleton.

scholarly journals Extract of grape seed enhances the growth performance, humoral and mucosal immunity, and resistance of common carp (Cyprinus carpio) against Aeromonas hydrophila

2020 ◽  
Vol 0 (0) ◽  
Author(s):  
Zohre Mehrinakhi ◽  
Ehsan Ahmadifar ◽  
Najmeh Sheikhzadeh ◽  
Mohsen Shahriari Moghadam ◽  
Mahmoud A.O. Dawood
Keyword(s):  
Disease Resistance ◽  
Growth Performance ◽  
Common Carp ◽  
Cyprinus Carpio ◽  
Aeromonas Hydrophila ◽  
Hematological Parameters ◽  
Control Diet ◽  
Grape Seed ◽  
Control Group ◽  
Feed Conversion

AbstractThis study evaluated the effects of grape seed extract (GSE) on the growth performance, immune response, and disease resistance of common carp (Cyprinus carpio). Fish were distributed in four groups and fed diets with varying levels of GSE at 0, 10, 20, and 30 g/kg for 56 days. The final length in fish fed with 20 and 30 g GSE/kg diet significantly increased in comparison with the control group (P<0.05). The final weight and weight gain of fish fed with GSE were statistically higher than the control diet, while the feed conversion ratio was lower in GSE groups than the control. Hematological parameters did not statistically enhance with GSE supplemented diets (P>0.05). However, fish fed with 30 g GSE/kg diet displayed a higher WBC count significantly than the other group (P<0.05). The serum total protein and globulin in fish fed with 20 g GSE/kg diet significantly increased compared to the control (P<0.05). A similar pattern was noticed in results for serum and mucus lysozyme activity with a significant increase in fish fed with 20 and 30 g GSE/kg diet compared to the control (P<0.05). Total antibody levels in serum and mucus samples were also increased in fish by dietary GSE with maximum levels by 20 and 30 g GSE/kg diet. Besides, mucus protease activity was higher in fish received 20 and 30 g GSE/kg diet with maximum level shown in fish fed with 30 g GSE/kg diet compared to the control group (P<0.05). Meanwhile, all groups showed higher mucus antibacterial activity against Aeromonas hydrophila with the highest activity in 30 g GSE/kg diet in comparison with the control group. The cumulative mortality was 36.67% when fish fed with 30 g GSE/kg diet and challenged with A. hydrophila. However, the mortality rate was 40% and 53.33% in fish fed 20 and 30 g GSE/kg diet, respectively. This study suggests that supplementation of GSE could significantly enhance the growth performance, immune responses, and disease resistance against A. hydrophila in common carp.

scholarly journals Utilization of biofloc meal as a feed ingredient for Nile tilapia and common carp

2018 ◽  
Vol 17 (1) ◽  
pp. 9 ◽  
Author(s):  
Julie Ekasari ◽  
Hilda Kemala Pasha ◽  
Nur Bambang Priyoutomo
Keyword(s):  
Growth Rate ◽  
Common Carp ◽  
Fish Species ◽  
Cyprinus Carpio ◽  
Oreochromis Niloticus ◽  
Nile Tilapia ◽  
Control Diet ◽  
Control Treatment ◽  
Lower Growth ◽  
Similar Treatment

<p align="center"><strong>ABSTRACT</strong></p><p>This study was aimed to evaluate the utilisation of biofloc meal collected from biofloc-based catfish intensive culture as a mix ingredient for Nile tilapia <em>Oreochromis niloticus</em> and common carp <em>Cyprinus carpio </em>diet. A control diet containing 29.03% crude protein was used in this experiment. Experimental diet was made by mixing 30% biofloc waste meal with the control diet and repelleted after the addition of 2% of binder. To determine the experimental feed digestibility, 0.5% of Cr<sub>2</sub>O<sub>3 </sub>was added as a marker for digestibility. The feed was offered to satiation at a frequency of 3 times a day for 28 days of experimentation. Nile tilapia and common carp juveniles with an initial average body weight of 11.72±0.04 g and 8.81±0.04 g, respectively, were used as the experimental animals. Each fish species were randomly stocked with a density of 10 fish/aquarium (30´45´30 cm<sup>3</sup>). The results showed that dry matter digestibility of diets with 30 % biofloc waste meal in both fish species were significantly lower than those of the controls (P&lt;0.05). However, protein, lipid and phosphorus digestibilities of diets containing biofloc waste meal were significantly higher than those of the controls (P&lt;0.05). Feeding with biofloc waste meal mixed feed to tilapia resulted in lower growth rate compared to that to fed control feed. On the other hand, similar treatment to common carp resulted in comparable growth rate to the control treatment.</p><p>Keywords: biofloc meal, digestibility, growth performance, tilapia, common carp</p><p> </p><p class="Default" align="center"><strong>ABSTRAK</strong></p><p class="Default"><strong>                                                                                      </strong></p><p class="Paragraf">Penelitian ini bertujuan untuk mengevaluasi pemanfaatan tepung bioflok yang dikumpulkan dari limbah pemeliharaan ikan lele intensif berbasis teknologi bioflok sebagai campuran pakan untuk ikan nila <em>Oreochromis niloticus</em> dan ikan mas <em>Cyprinus carpio</em>. Pakan kontrol yang digunakan adalah pakan komersial dengan kadar protein 29,03%. Pembuatan pakan uji dilakukan dengan mencampurkan tepung limbah bioflok (30%) dengan pakan kontrol (67,5%) dan dibentuk pelet kembali setelah dilakukan penambahan <em>binder</em> sebanyak 2% dan Cr<sub>2</sub>O<sub>3 </sub>sebanyak 0,5% sebagai penanda untuk menganalisis kecernaan pakan dengan tepung limbah bioflok. Pakan diberikan secara <em>at satiation </em>dengan frekuensi pemberian pakan 3 kali/hari selama 28 hari pemeliharaan. Bobot rata-rata ikan awal adalah 11,72±0,04 g untuk ikan nila, dan 8,81±0,04 g untuk ikan mas dengan kepadatan awal masing-masing 10 ekor/akuarium (30´45´30 cm<sup>3</sup>). Hasil penelitian ini menunjukkan bahwa nilai kecernaan total pakan dengan tepung limbah bioflok baik pada ikan nila maupun ikan mas lebih rendah daripada pakan kontrol (P&lt;0,05). Namun demikian, kecernaan protein, lemak, dan fosfor pakan dengan campuran tepung limbah bioflok lebih tinggi daripada kontrol (P&lt;0,05). Pemberian tepung limbah bioflok sebanyak 30% sebagai campuran pakan menghasilkan laju pertumbuhan spesifik ikan nila yang lebih rendah (P&lt;0,05), sedangkan perlakuan yang sama pada ikan mas memberikan laju pertumbuhan spesifik yang tidak berbeda nyata dengan kontrol (P&gt;0,05).</p><p class="Paragraf"> </p><p class="Paragraf">Kata kunci: tepung limbah bioflok, kecernaan, kinerja pertumbuhan, ikan nila, ikan mas</p>

Enrichment of common carp (Cyprinus carpio) diet with Malic acid: Effects on skin mucosal immunity, antioxidant defecne and growth performance

2020 ◽  
Vol 0 (0) ◽  
Author(s):  
Roghieh Safari ◽  
Seyed Hossein Hoseinifar ◽  
Maryam Dadar ◽  
Hien Van Doan
Keyword(s):  
Common Carp ◽  
Cyprinus Carpio ◽  
Malic Acid ◽  
Control Group ◽  
Skin Mucus ◽  
Antioxidant Genes ◽  
Treatment Comparison ◽  
Genes Expression ◽  
Significant Difference ◽  
And Control

AbstractThe present study investigated possible effects of dietary malic acid on the expression of immunity, antioxidant and growth related genes expression as well as skin mucus immune parameters in common carp. Common carp (Cyprinus carpio) fingerlings were fed diets supplemented with different levels (0 [control], 0.5%, 1%, 2%) of malic acid (MA) for 60 days. The results revealed highest expression levels of immune-related genes (tnf-alpha, il1b, il8 and lyz) in skin of common carp fed 2% MA (P < 0.05). Regarding 1% MA treatment comparison with control group, significant difference was noticed just in case of lyz (P < 0.05). Evaluation of growth related genes expression revealed no significant difference between treatments (P > 0.05). The study of antioxidant related genes (gsta and gpx) in common carp skin fed with MA, showed significant difference between treated groups and control (P < 0.05). Carps fed with 2% MA had highest alkaline phosphatase activity in skin mucus compared other treated groups and control (P < 0.05). There were no significant difference between 0.5% and 1% and control (P > 0.05). The study of total protein and total immunoglobulin (Ig) in common carp skin musus revealed no alteration following MA treatment (P > 0.05). The present data demonstrated that feeding with MA altered immune and antioxidant genes expression in skin mucus of common carp.

scholarly journals Acetylcholinesterase enzyme activity in carp brain and muscle after acute exposure to diafuran

2008 ◽  
Vol 65 (4) ◽  
pp. 340-345 ◽  
Author(s):  
Jaqueline Ineu Golombieski ◽  
Enio Marchesan ◽  
Edinalvo Rabaioli Camargo ◽  
Joseânia Salbego ◽  
Joele Schmitt Baumart ◽  
...  
Keyword(s):  
Common Carp ◽  
Cyprinus Carpio ◽  
Grass Carp ◽  
Ache Activity ◽  
Bighead Carp ◽  
Ctenopharyngodon Idella ◽  
Control Treatment ◽  
Aristichthys Nobilis ◽  
Early Biomarker ◽  
The Common

Sublethal adverse effects may result from exposure of aquatic organisms to insecticides at environmentally relevant concentrations. Fingerlings of the common carp (Cyprinus carpio, Linnaeus, 1758), grass carp (Ctenopharyngodon idella, Valenciennes, 1844), and bighead carp (Aristichthys nobilis, Richardson, 1845) were exposed to diafuran, an insecticide widely used during rice cultivation in Southern Brazil. The aim of this study was to verify the relationship between the lethal concentration (LC50) of diafuran and the acetylcholinesterase (AChE) activity in brain and muscle tissues of these species as a possible early biomarker of exposure to this insecticide. LC50 was determined for fish exposed to diafuran concentrations during 96 h (short term): common carp: control, 0.5, 1.0, 1.5, 2.0, 2.5 and 3.0 mg L-1; grass carp: control, 1.0, 2.0, 3.0 and 3.5 mg L-1 and, bighead carp: control, 0.5, 1.0, 1.5, 2.0, 3.0 and 4.0 mg L-1, as well as the determination of AChE at concentrations near LC50 for these species. LC50 values (nominal concentrations) were 1.81 mg L-1 for the common carp, 2.71 mg L-1 for the grass carp and, 2.37 mg L-1 for the bighead carp. All carps exposed to diafuran were lethargic (lower concentrations) or immobile. Diafuran inhibited the acetylcholinesterase activity in brain (~38%) and muscle (~50%) of all species. Muscle of bighead carp under control treatment showed higher specific AChE activity than brain (14.44 against 5.94 µmol min-1 g protein-1, respectively). Concentrations of diafuran used for rice cropping may affect Cyprinus carpio, Ctenopharyngodon idella and Aristichthys nobilis behaviors and the AChE activities in brain and muscle of these species may be an early biomarker of toxicity of this insecticide.

scholarly journals Effect of β-glucan on behavioral, biochemical and hematological parameters against toxicity of copper sulfate in common carp Cyprinus carpio L.

2014 ◽  
Vol 38 (2) ◽  
pp. 128-137
Author(s):  
Jamal K. Al-faragi,
Keyword(s):  
Common Carp ◽  
Copper Sulfate ◽  
Cyprinus Carpio ◽  
Hematological Parameters ◽  
Control Group ◽  
Enzymes Activity ◽  
Common Carp Cyprinus Carpio ◽  
Carp Cyprinus Carpio ◽  
Wbc Count ◽  
Hb Concentration

The aim of the present study was to assess the effect of a commercial ß-glucan against acute toxic effect of copper sulfate on common carp Cyprinus carpio L. behavioral, hematological parameters and biochemical tests. A total of 250 Fish (150 ± 2 g) were fed a basal control diet or the basal diet plus a ß-glucan (1 g kg-1 diet) for 60 days. LC50 was calculated for 72 h which was 10.83 mg L-1.  At the end of the trial, CuSO4.5H2O was added to all treatments  at a dose  of  5 mgL-1  for  T1  and  T2, 7mg L-1  for  T3  and  T4, 9  mgL-1 for T5  and  T6   for  96h. Fish behavioral changes were recorded during 96h of exposure to different Cu concentrations with and without β-glucan, which showed abnormalities such as increase swimming activity, jerky movement, loss of equilibrium, respiratory stress, increase operculum movement, frequent jumping, erratic swimming and swimming near the water surface. On the other hand, ß-glucan supplemented groups showed significant increase (P≤0.05) in Hb concentration, PCV%, RBC and WBC count in comparison with groups without ß-glucan. The results of dietary groups without ß-glucan showed a significant decrease (P≤ 0.05) in Hb concentration, hematocrit value, RBC count, but there were significant increase (P≤ 0.05) in WBC count with an increase of Cu concentration compared to control groups. Results showed significant increase (P≤ 0.05) in dietary groups without ß-glucan in values of ALT, AST and ALP enzymes activity with elevation of Cu levels compared to control group. But, ß-glucan supplemented groups showed significant decrease (P ≤ 0.05) in values of ALT, AST and ALP enzymes activity in comparison with dietary groups without ß-glucan. These results suggested that β-glucan has protective effect against CuSO4 toxicity and future studies should be pursued particularly immune response and molecular studies

Effect of royal jelly on milk composition and blood biochemical parameters in lactating ewes

2019 ◽  
Vol 157 (2) ◽  
pp. 176-181
Author(s):  
Mahmoud S. El-Tarabany ◽  
Akram A. El-Tarabany ◽  
Mostafa A. Atta ◽  
Omar A. Ahmed-Farid ◽  
Mohamed M. Mostafa
Keyword(s):  
Milk Fat ◽  
Nutritive Value ◽  
Royal Jelly ◽  
Antioxidant Activities ◽  
Basal Diet ◽  
Milk Composition ◽  
Feed Additives ◽  
Control Treatment ◽  
Control Group ◽  
Blood Biochemical

AbstractUse of antibiotics as feed additives has been reduced to avoid the hazard of drug residues, and consequently, the search for alternative natural additives has developed. Thus, the aim was to evaluate the influence of royal jelly (RJ) supplementation on milk composition, blood biochemical and antioxidant parameters of lactating ewes. Thirty-six Ossimi ewes were divided randomly into two groups (18 animals each). For a period of 4 weeks, the control group (CON) was fed a basal diet only, while the other group was fed the basal diet and supplemented with a single bolus of RJ (1000 mg/head). The RJ-supplemented ewes produced significantly higher milk protein, fat and total solids than the CON group. The RJ group had a significantly higher red blood cell count, haemoglobin content, haematocrit value and total leucocyte counts, but lower neutrophil to lymphocyte ratio when compared with the control treatment. The RJ group showed significantly higher concentrations of total antioxidant capacity, superoxide dismutase activity and glutathione in the serum compared with the control treatment. In conclusion, RJ supplements can improve the nutritive value of milk fat and the serum antioxidant activities in lactating ewes.

scholarly journals Dry matter intake observed and predicted by the nutritional systems NRC and BR-Corte for feedlot nelore heifers fed with human feed products

2019 ◽  
Vol 40 (6Supl2) ◽  
pp. 3157
Author(s):  
Simone Pedro da Silva ◽  
Carina Ubirajara de Faria ◽  
Ana Caroline Rodrigues da Cunha ◽  
Aline Maria Soares Ferreira ◽  
Jean Marcos Castro Paula ◽  
...  
Keyword(s):  
Dry Matter ◽  
Control Diet ◽  
Dry Matter Intake ◽  
Daily Weight Gain ◽  
Control Treatment ◽  
Control Group ◽  
Human Diet ◽  
Lower Growth ◽  
The Difference ◽  
Average Body

We aimed to evaluate the effects of using a concentrated ration containing industrialised human food on the productive parameters of Nellore heifers in confinement, in addition to comparing the dry matter intake (DMI) observed with that predicted by the nutritional systems BR-Corte and NRC, in order to determine which system makes the most accurate predictions for heifers of the breed Nelore in feedlots. Twenty-four Nelore heifers, with an average age of 24 months and average body weight (BW) of 350 kg, were used over the 70 days of the experiment. They were randomly assigned to two treatments and housed in partially covered stalls equipped with an automatic Growsafe® Systems feeding system. Animals in the control group (CONT) received a concentrated ration containing ground corn, soybean meal, urea, and mineral nucleus. In the residual treatment (RES) group, heifers received concentrated feed containing products from the human diet. The difference between the treatments was the concentrated ration; in the control treatment, the energy source used was maize, whereas in the residue treatment, the energy sources were corn chips, coconut candies, and ground coffee. Corn silage was included as forage for both treatments. The forage:concentrate (F:C) ratio was 84:16. The predicted intake was estimated according to the nutritional systems NRC (2000, 2016) and BR-Corte (VALADARES FILHO et al., 2010, 2016). The average DMI observed of the heifers was 7.46 kg day-1. Heifers fed with human feed residue had a higher dry matter intake (DMI) and higher ether extract intake (EEI) than animals fed with the control feed. In addition, heifers that received human feed products showed a lower average daily weight gain (DWG) of 1.08 kg day-1 than animals fed the control treatment with an average DWG of 1.24 kg day-1. The predicted dry matter intake by NRC 2000 was an overestimate by 9.78 % and by BR-Corte 2010 was an overestimate by 5.5 %. The NRC 2016 and BR-Corte 2016 underestimated the intake of heifers by 18.9 % and 7.1 %, respectively. It was concluded that growing Nelore heifers fed with human feed products, with a F:C ratio of 84:16, present a higher DMI and lower growth performance and feed efficiency than heifers fed the control diet. The DMI predicting models for Nellore heifers proposed by BR-Corte are more adequate than the NRC models.

Effect of dietary supplementation of salts of organic acids on gut morphology and meat quality of broilers

2017 ◽  
Author(s):  
Vinus Vinus ◽  
M. A. Akbar ◽  
B. S. Tewatia ◽  
Sushil Kumar
Keyword(s):  
Organic Acids ◽  
Meat Quality ◽  
Control Diet ◽  
Feed Additives ◽  
Metabolizable Energy ◽  
Coliform Bacteria ◽  
Control Group ◽  
Gut Morphology ◽  
Salts Of Organic Acids

The present investigation was conducted to appraise the effect of different levels of salts of organic acids on the gut morphology and meat quality of broilers. A total 300 day-old commercial chicks were randomly divided into 5 treatments. Each treatment consists of 6 replicates having 10 birds per replicate. The control diet was formulated to contain approximately the same metabolizable energy (ME), crude protein and limiting amino acids (methionine and lysine) as per the requirements of birds. The first group (T1) was fed on control diet without any feed additives, while, groups (T2, T3, T4 and T5) were fed on basal diets containing sodium butyrate and calcium propionate @ 0.5% and 1.0% respectively. Six birds from each treatment (1 per replication) were randomly selected for slaughter and collection of samples and microbial study at day 42. Performance of birds in terms of body weight and feed conversion ratio was significantly improved in treated groups. Results showed that in gut morphology, pH was reduced significantly (P less than 0.05) in T3 and T5 (6.24 and 6.22, respectively) as compared to the control group (6.64) while significant improvement was observed in villus height and crypt depth in all treatment groups and were highest in T3 (539.50µm,135.67 µm). Lowest Coliform bacteria (log CFU/g.)was recorded in T3 (16.57) which was significantly differ with the T1 (19.17) and Lactobacilli count was significantly increased in all treatments as compared to control.Supplementation of organic acids reduced meat cholesterol level significantly and lowest was in T5(48.03mg/dL) followed by T3 (48.30mg/dL). Similarly SFA decreased while PUFA content was increased significantly on supplementation of salts as compared to control.

scholarly journals Survival of common carp carrying Cyca-DAB1*05 post-challenged with Aeromonas hydrophila

2015 ◽  
Vol 13 (2) ◽  
pp. 167
Author(s):  
Laode Muhammad Arsal ◽  
Munti Yuhana ◽  
Sri Nuryati ◽  
, Alimuddin
Keyword(s):  
Major Histocompatibility Complex ◽  
Molecular Marker ◽  
Common Carp ◽  
Cyprinus Carpio ◽  
Aeromonas Hydrophila ◽  
Control Treatment ◽  
Control Fish ◽  
Complex Ii ◽  
Histocompatibility Complex ◽  
Blood Profiles

<p class="BasicParagraph" align="center"><strong>ABSTRACT</strong></p><p class="BasicParagraph" align="center"><strong> </strong></p><p class="Pa2">Blood parameters are considered as important indicators to diagnose fish health status. This study was performed to observe blood profiles including total erythrocytes, hemoglobin concentration, hematocrite, total leukocytes and differential leukocytes, and survival of common carp <em>Cyprinus carpio </em>infected by <em>Aeromonas hydrophila</em>. Fish were divided into two groups: the 3rd generation of common carp carrying fish carrying Cyca-DAB1*05 of major histocompatibility complex II molecular marker, and fish without the marker as control treatment. histocompatibility complex II molecular marker, and fish without the marker as control treatment. Common carp 3rd generation was produced by crossing among second generation of fish carrying the Cyca-DAB1*05 marker. Each fish was injected intramuscularly by 0.1 mL of 108 cfu/mL <em>A. hydrophila</em>. Challenge test was conducted for 14 days and blood was collected at day-0, three, seven, and 14. The results of this study showed that erythrocytes, hemoglobin and hematocrite concentrations of common carp carrying the molecular marker at post challenge with <em>A. hydrophila </em>were higher (P&lt;0.05) compared to control fish. The blood profiles were highly correlated to survival of fish. Survival of fish that carrying the molekuler marker was about two point six fold higher than those of control fish.</p><p class="Default"> </p><p class="Default">Keywords: <em>Cyprinus carpio</em>, <em>Aeromonas hydrophila</em>, Cyca-DAB1*05, molecular marker</p><br /><p class="BasicParagraph"> </p><p class="BasicParagraph"> </p><p class="BasicParagraph" align="center"><strong>ABSTRAK</strong></p><p class="BasicParagraph"><strong> </strong></p><p class="Pa2">Gambaran darah merupakan indikator penting untuk mendiagnosa penyakit ikan. Penelitian ini dilakukan untuk meguji gambaran darah ikan mas <em>Cyprinus carpio </em>setelah diinfeksi dengan bakteri <em>Aeromonas hydrophila </em>yang meliputi total sel darah merah, konsentrasi hemoglobin, hematokrit, total sel darah putih, dan diferensial leukosit, serta sintasannya. Ikan mas yang digunakan terdiri atas ikan mas generasi ketiga yang membawa marka molekuler Cyca-DAB1*05 dari kelompok <em>major histocompatibility complex </em>II dan ikan mas tanpa marka sebagai kontrol. Ikan mas generasi ketiga merupakan keturunan persilangan antarikan mas generasi kedua yang mempunyai marka Cyca-DAB1*05. Masing-masing ikan diinfeksikan <em>A. hydrophila </em>secara intramuskuler pada dosis 0,1 mL, kepadatan 108 cfu/mL. Uji tantang dilakukan selama 14 hari, dan sampel darah ikan diambil pada hari ke-0, tiga, tujuh dan 14. Hasil uji tantang menunjukkan bahwa total sel darah merah, konsentrasi hemoglobin dan hematokrit pada ikan mas yang membawa marka molekuler lebih tinggi dibandingkan ikan mas tanpa marka (P&lt;0,05). Hasil uji gambaran darah berkorelasi tinggi dengan tingkat kelangsungan hidup ikan mas. Tingkat kelangsungan hidup ikan mas yang membawa marka molekuler Cyca-DAB1*05 lebih tinggi hingga dua koma enam kali daripada ikan mas kontrol.</p><p class="Default"> </p><p class="Pa2">Kata kunci: <em>Cyprinus carpio</em>, <em>Aeromonas hydrophila</em>, Cyca-DAB1*05, marka molekuler</p><br /><p> </p>

scholarly journals Impact of Black Grape byproducts on Blood Picture in Common Carp (Cyprinus carpio L.)

2017 ◽  
Vol 30 (1) ◽  
pp. 32-37
Author(s):  
Pola J. Nader ◽  
Nasreen M. Abdulrahman
Keyword(s):  
Common Carp ◽  
Cyprinus Carpio ◽  
White Blood Cells ◽  
Diet Group ◽  
Control Group ◽  
Mean Corpuscular Hemoglobin ◽  
Fish Farms ◽  
Common Carp Cyprinus Carpio ◽  
Carp Cyprinus Carpio ◽  
Feeding Trials

This experiment was conducted for 70 days on 165 common carp Cyprinus carpio fingerlings which were brought from local fish farms located in Daqooq, Haftaghar, Al-Tameem province. Fishes were distributed among experimental tanks with mean initial weight of 24.09 gm. They were pre-acclimated to laboratory conditions for 21 days prior to the feeding trials. The experiment was conducted by using 15 plastic tanks (100 L) representing five treatments with three replicates. Each tank was stocked with 11 fishes. Treatments included 0, 2.5, 5.0, 7.5 or 10 gm grape byproduct/ kg diet. The result showed that red blood cell (RBC) data of the control group was significantly higher than other treatments. Hemoglobin of fishes in groups 2.5 and 10 gm/ kg diet was significantly different from other treatments. Mean corpuscular hemoglobin (MCH) values for 0.0, 5.0 and 10 gm/kg groups were significantly different, so mean corpuscular volume (MCV) values showed significant differences resulted from 0.0, 5.0, 7.5 and 10 gm/ kg diet compared to 2.5 gm/ kg diet group. The white blood cells (WBC) were significantly different in 2.5, 5.0 and 7.5 gm/ kg diet. Granulocyte percentages obtained in all treatments were significantly different from control. Lymphocyte percentages were significantly different in all treatments compared to 10 gm/ kg diet group. The Monocytes were significantly different in 2.5, 7.5 and 10 gm/ kg groups.

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