scholarly journals Puerarin Improves Diabetic Aorta Injury by Inhibiting NADPH Oxidase-Derived Oxidative Stress in STZ-Induced Diabetic Rats

2016 ◽  
Vol 2016 ◽  
pp. 1-9 ◽  
Author(s):  
Wenping Li ◽  
Wenwen Zhao ◽  
Qin Wu ◽  
Yuanfu Lu ◽  
Jingshan Shi ◽  
...  
Keyword(s):  
Nadph Oxidase ◽  
Colorimetric Assay ◽  
Serum Insulin ◽  
Fasting Blood Glucose ◽  
Serum Levels ◽  
Rat Aorta ◽  
Diabetic Rats ◽  
Diabetic Rat ◽  
Positive Staining ◽  
Glucose Levels

Objective.Puerarin is a natural flavonoid isolated from the TCM lobed kudzuvine root. This study investigated the effect and mechanisms of puerarin on diabetic aorta in rats.Methods.Streptozotocin- (STZ-) induced diabetic rats were administered with puerarin for 3 weeks. Levels of serum insulin (INS), PGE2, endothelin (ET), glycated hemoglobin (GHb), H2O2, and nitric oxide (NO) in rats were measured by ELISA and colorimetric assay kits. The aortas were stained with H&E. Moreover, the mRNA expression of ICAM-1, LOX-1, NADPH oxidase 2 (NOX2), and NOX4 and the protein expression of ICAM-1, LOX-1, NF-κB p65, E-selectin, NOX2, and NOX4 in aorta tissues were measured by real-time PCR and Western blot, respectively. The localization of ICAM-1, NF-κB p65, NOX2, and NOX4 in the aorta tissues was also determined through immunohistochemistry.Results.Puerarin treatment exerted no effect on fasting blood glucose levels but significantly reduced the serum levels of INS, GHb, PGE2, ET, H2O2, and NO. In addition, puerarin improved the pathological alterations and inhibited the expression of ICAM-1, LOX-1, NOX2, and NOX4 at both mRNA and protein levels. Puerarin also significantly reduced the number of cells showing positive staining for ICAM-1, NOX2, NOX4, and NF-κB p65.Conclusion.Puerarin demonstrated protective effect on the STZ-induced diabetic rat aorta. The protective mechanisms may include regulation of NF-κB and inhibition of NOX2 and NOX4 followed by inhibition of cell adhesion molecule expression.

scholarly journals Antidiabetic potential of the leaf extract of Centella asiatica in alloxaninduced diabetic rats

2016 ◽  
Vol 4 (1) ◽  
pp. 51-59 ◽  
Author(s):  
Talha Bin Emran ◽  
Mycal Dutta ◽  
Mir Muhammad Nasir Uddin ◽  
Aninda Kumar Nath ◽  
Md Zia Uddin
Keyword(s):  
Body Weight ◽  
Leaf Extract ◽  
Total Cholesterol Level ◽  
Serum Insulin ◽  
Fasting Blood Glucose ◽  
Centella Asiatica ◽  
Liver Glycogen ◽  
Diabetic Control ◽  
Diabetic Rats ◽  
Diabetic Rat

The study was designed to evaluate the glucose and cholesterol lowering effect of the aqueous extract of Centella asiatica leaf using the alloxan-induced diabetic rats and compared the activity with diabetic control and antidiabetic drug (Glibenclamide). Leaf extract (50 mg/kg) of C. asiatica and Glibenclamide were administered to normal and experimental diabetic rats for the duration of 10 days. In the alloxan-induced diabetic rat model, C. asiatica extract (50 mg/kg) significantly (p < 0.05) lowered the fasting blood glucose level as well as the total cholesterol level. Serum insulin levels were not stimulated in the animals treated with the extract. In addition, changes in body weight, serum lipid profiles and liver glycogen levels assessed in the extract treated diabetic rats were compared with diabetic control and normal animals. Significant results (p < 0.05) were observed in the estimated parameters. Surprisingly, body weight was increased significantly (p < 0.05) in the C. asiatica treated diabetic group. Phytochemical screening showed the presence of alkaloids, flavonoids, glycosides, steroids and tannins in significant amountsJahangirnagar University J. Biol. Sci. 4(1): 51-59, 2015 (June)

scholarly journals Anti-Allergic, Anti-Inflammatory and Anti-Hyperglycemic Activity of Chasmanthe aethiopica Leaf Extract and Its Profiling Using LC/MS and GLC/MS

Plants ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 1118
Author(s):  
Iriny M. Ayoub ◽  
Michal Korinek ◽  
Mohamed El-Shazly ◽  
Bernhard Wetterauer ◽  
Hesham A. El-Beshbishy ◽  
...  
Keyword(s):  
Leaf Extract ◽  
Biological Activities ◽  
Serum Insulin ◽  
Fasting Blood Glucose ◽  
Significant Inhibition ◽  
Binding Energies ◽  
Diabetic Rat ◽  
Glucose Levels ◽  
Anti Inflammatory

This study aims to comprehensively explore the phytoconstituents as well as investigate the different biological activities of Chasmanthe aethiopica (Iridaceae) for the first time. Metabolic profiling of the leaf methanol extract of C. aethiopica (CAL) was carried out using HPLC-PDA-ESI-MS/MS. Twenty-nine compounds were annotated belonging to various phytochemical classes including organic acids, cinnamic acid derivatives, flavonoids, isoflavonoids, and fatty acids. Myricetin-3-O-rhamnoside was the major compound identified. GLC/MS analysis of the n-hexane fraction (CAL-A) resulted in the identification of 45 compounds with palmitic acid (16.08%) and methyl hexadecanoic acid ester (11.91%) representing the major constituents. CAL-A exhibited a potent anti-allergic activity as evidenced by its potent inhibition of β-hexosaminidase release triggered by A23187 and IgE by 72.7% and 48.7%, respectively. Results were comparable to that of dexamethasone (10 nM) in the A23187 degranulation assay showing 80.7% inhibition for β-hexosaminidase release. Both the n-hexane (CAL-A) and dichloromethane (CAL-B) fractions exhibited potent anti-inflammatory activity manifested by the significant inhibition of superoxide anion generation and prohibition of elastase release. CAL showed anti-hyperglycemic activity in vivo using streptozotocin-induced diabetic rat model by reducing fasting blood glucose levels (FBG) by 53.44% as compared with STZ-treated rats along with a substantial increase in serum insulin by 22.22%. Molecular modeling studies indicated that dicaffeoylquinic acid showed the highest fitting with free binding energies (∆G) of −47.24 and −60.50 Kcal/mol for human α-amylase and α-glucosidase, respectively confirming its anti-hyperglycemic activity. Thus, C. aethiopica leaf extract could serve as an effective antioxidant natural remedy combating inflammation, allergy, and hyperglycemia.

scholarly journals Influence of Ocimum sanctum (L.) Extract on the Activity of Gliclazide in Alloxan-induced Diabetes in Rats

2020 ◽  
Vol 71 (11) ◽  
pp. 101-110
Author(s):  
Tayyeba Ramzan ◽  
Bilal Aslam ◽  
Faqir Muhammad ◽  
Muhammad Naeem Faisal ◽  
Asif Hussain
Keyword(s):  
Body Weight ◽  
Blood Glucose ◽  
Liver Tissue ◽  
Fasting Blood Glucose ◽  
Serum Levels ◽  
Diabetic Rats ◽  
Ocimum Sanctum ◽  
Protective Effects ◽  
Oxidative Stress Biomarkers ◽  
Glucose Levels

Ocimum sanctum L. is widely used as traditional remedy to manage hyperglycemia. This study was aimed to evaluate the effect of aqueous-methanolic extract of O. sanctum leaves (OSE) on the anti-diabetic activity of gliclazide in alloxan-induced diabetes in Wistar rats. Diabetes was induced by intraperitoneally injecting alloxan (120 mg/kg b.w.) in rats. Treatments including OSE (100 mg/kg b.w.), gliclazide (100 mg/kg b.w.), and in combination were given daily to diabetic rats till the 21st day of study. Body weight and fasting blood glucose levels were determined at regular intervals, while blood and organ samples were taken at the end of the study for biochemical and histopathological studies. Results showed that treatments exhibited anti-hyperglycemic activity through significantly (p [ 0.05) restoring body weight, fasting blood glucose level, and serum levels of glucose, insulin and HbA1c. The anti-lipidemic activity was noticed as total cholesterol (TC), triglyceride (TG), high-/low density-lipoproteins (HDL-C, LDL-C) levels were restored in treated diabetic rats. Ameliorative effects of treatments were observed as significant (p [ 0.05) reduction in serum levels of liver function biomarkers (alanine aminotransferase; ALT, aspartate aminotransferase; AST, alkaline phosphatase; ALP and bilirubin; BIL) and restoration of oxidative stress biomarkers (catalase; CAT, superoxide dismutase; SOD and malondialdehyde; MDA) in liver tissue. Histopathological findings supported these results as an increase in pancreatic islets size and protective effects on liver tissue was observed in diabetic rats treated with gliclazide and OSE alone and their combination. Conclusively, the combination of OSE and gliclazide produced a synergistic anti-diabetic effect as compared to that of alone treatment.

scholarly journals Effect of a novel succinamic acid derivative as potential anti-diabetic agent in experimental diabetic rats

2018 ◽  
Vol 8 (6-s) ◽  
pp. 57-62
Author(s):  
Nikhil Khurana ◽  
Pankaj Sharma ◽  
Sunita Bhagat ◽  
Suman Bala Sharma
Keyword(s):  
Lipid Peroxidation ◽  
Serum Lipid ◽  
Serum Insulin ◽  
Fasting Blood Glucose ◽  
Diabetic Rats ◽  
Glucose Levels ◽  
Blood Glucose Levels ◽  
Succinamic Acid ◽  
Oxobutanoic Acid

4-((benzyloxy) amino)-2-hydroxy-4-oxobutanoic acid which is a succinamic acid derivative has been synthesized in 3 step reaction with malic acid. Its structure confirmation was done by various techniques like 1H NMR, 13C NMR, & HRMS and is recently proposed as an insulinotropic agent for the treatment of non-insulin dependent diabetes mellitus. In the present study, the effect of 4-((benzyloxy) amino)-2-hydroxy-4-oxobutanoic acid on plasma glucose, serum insulin, serum lipid profile and lipid peroxidation in streptozotocin–nicotinamide induced type 2 diabetic model was investigated.  4-((benzyloxy) amino)-2-hydroxy-4-oxobutanoic acid was administered orally (20 mg/kg b.w.) to streptozotocin + nicotinamide (STZ + NAD) induced diabetic rats for 28 days. A significant increase in fasting blood glucose levels, HbA1c levels, Serum lipid profile (TG & TC) and in  the levels of Malonaldialdehyde (MDA, end product of lipid peroxidation) was observed in STZ +NAD diabetic rats whereas the levels of high density lipoprotein-cholesterol (HDL-C) and serum insulin levels were significantly decreased  in STZ + NAD induced diabetic rats The effect of 4-((benzyloxy)amino)-2-hydroxy-4-oxobutanoic acid was compared with glibenclamide, a reference drug. Treatment with 4-((benzyloxy) amino)-2-hydroxy-4-oxobutanoic acid and glibenclamide resulted in a significant reduction of fasting blood glucose levels with increase in plasma insulin levels in diabetic treated rats. 4-((benzyloxy) amino)-2-hydroxy-4-oxobutanoic acid also resulted in a significant improvement in serum lipids and lipid peroxidation products. Our results suggest the potential role of 4-((benzyloxy) amino)-2-hydroxy-4-oxobutanoic acid in the management of type-2 diabetes mellitus experimental rats. Keywords: 4-((benzyloxy) amino)-2-hydroxy-4-oxobutanoic acid, dyslipidemia, streptozotocin induced diabetes, lipid peroxidation

scholarly journals The synergistic reno-protective effect of Rhizophora mucronata and Avicennia marina aqueous extracts against diabetes induced renal changes

2020 ◽  
Vol 9 (9) ◽  
pp. 1317
Author(s):  
Obidallah Hamdan Ali Al-Jaghthmi ◽  
Isam El Din Mohamed El Amin Abu Zeid ◽  
Hassan Mohammad Heba
Keyword(s):  
Apoptotic Cell ◽  
Serum Insulin ◽  
Fasting Blood Glucose ◽  
Avicennia Marina ◽  
Potential Effect ◽  
Diabetic Rats ◽  
Aqueous Extracts ◽  
Rhizophora Mucronata ◽  
Glucose Levels ◽  
Renal Changes

Background: The conventional medications of diabetes mellitus have many side effects that bring to diabetic cases, so there is a need for introducing natural remedies with antidiabetic effect. The objectives of this study aimed to evaluate the hypoglycemic and antidiabetic effects of aqueous extracts of a mixture of R. mucronata and A. marina against experimentally streptozotocin (STZ) induced diabetic rats and to assess its efficacy in alleviating diabetes-induced impact on kidney function.Methods: Sixty rats distributed into four groups (n=15) were used in this study. They included the control, STZ-induced diabetic, STZ-induced diabetic treated with a mixture of Rhizophora mucronata and Avicennia marina extract and non-diabetic group received a mixture of Rhizophora mucronata and Avicennia marina extract. After 6 weeks of treatment, the biochemical and histological alterations were investigated in all rats.Results: Daily oral administration of a mixture of R. mucronata and A. marina leaves extract to STZ-induced diabetic significant (p≤0.001) increase serum insulin levels and reduced fasting blood glucose levels, serum BUN and uric acid compared to the untreated diabetic rats. This mixture also improved the renal degeneration and inflammation induced by diabetes and reduced the number of the apoptotic cell in both renal cortex and medulla.Conclusions: The mixture of R. mucronata and A. marina extract significantly improve the diabetes-associated biochemical and histopathological renal changes indicating its potential effect as a natural nephroprotective agent against diabetic-induced nephropathy.

scholarly journals Hypoglycemic Activity and Antioxidative Stress of Extracts and Corymbiferin fromSwertia bimaculata In VitroandIn Vivo

2013 ◽  
Vol 2013 ◽  
pp. 1-12 ◽  
Author(s):  
Zhaoxia Liu ◽  
Luosheng Wan ◽  
Yuedong Yue ◽  
Zuoqi Xiao ◽  
Yutang Zhang ◽  
...  
Keyword(s):  
Serum Insulin ◽  
Low Density Lipoprotein ◽  
Fasting Blood Glucose ◽  
Density Lipoprotein ◽  
Glucose Consumption ◽  
Diabetic Rats ◽  
Oral Glucose ◽  
Glucose Levels ◽  
Blood Glucose Levels ◽  
Alternative Agent

The present study was to investigate the anti-diabetic activities ofSwertia bimaculata. Based on the glucose consumption ofS. bimaculataextractsand different fractions (petroleum, dichloromethane, ethyl acetate,n-butanol and water extracts) in 3T3-L1 adipocyte assay, ethanol (ETH) and dichloromethane (DTH) extracts had the most effective potency. Furthermore, ETH, DTH and corymbiferin (the most abundant component of DTH) were evaluated for anti-diabetic effects in high fat and sucrose fed combined with low dose streptozocin induced diabetic rats. DTH and corymbiferin displayed remarkable anti-diabetic activities. The fasting blood glucose levels were significantly decreased, while the serum insulin levels were obviously increased. The oral glucose tolerance was also improved. The lowed serum total cholesterol, low density lipoprotein (LDL) and triglyceride levels and increased ratio of HDL (high density lipoprotein)/LDL were observed. The insulin sensitivity was improved on the basis of increased expressions of insulin-receptor substrate-2, phosphatidylinositol 3-kinase and Ser/Thr kinase AKT2. And also DTH and corymbiferin improved antioxidant capacity and carbohydrate metabolism in diabetic rats, along with the improvement of histopathology of livers and pancreaticβcells. Corymbiferin was one of active constituents, responsible for anti-diabetic properties. Therefore,S. bimaculatacould be considered as an alternative agent against diabetes mellitus.

Anti-diabetic activity of diphenhydramine in diabetic rats

2020 ◽  
Vol 11 (4) ◽  
pp. 5067-5070
Author(s):  
Pang Jyh Chayng ◽  
Nurul Ain ◽  
Kaswandi Md Ambia ◽  
Rahim Md Noah
Keyword(s):  
Blood Glucose ◽  
Blood Glucose Level ◽  
Glucose Level ◽  
Fasting Blood Glucose ◽  
Insulin Level ◽  
Diabetic Rats ◽  
Group Iv ◽  
Diabetic Rat ◽  
Control Group ◽  
Group I

The purpose of this project is to study the anti-diabetic effect of on a diabetic rat model. A total of Twenty male Sprague rats were used and it randomly distributed into four groups which are Group I: , Group II: negative control, Group III: and Group IV: and . In diabetic model were induced with via injection at the dosage of 65mg/kg. and FBG (Fasting Blood Glucose) level of diabetic rats were assessed every three days. Blood was collected via cardiac puncture at day 21 after the induction of treatment. Insulin level of the rats was assessed with the Mercodia Rat Insulin ELISA kit. FBG level of group I (12.16 ±3.96, p&lt;0.05) and group IV (11.34 ±3.67, p&lt;0.05) were significantly decreased. Meanwhile, the for all rats did not show any significant increase. However, the insulin level was escalated in group IV (0.74+0.25, p&lt;0.05) significantly. The present study shows that the and the combination of and lowered blood glucose level and enhanced insulin secretion.

Sequoyitol ameliorates diabetic nephropathy in diabetic rats induced with a high-fat diet and a low dose of streptozotocin

2014 ◽  
Vol 92 (5) ◽  
pp. 405-417 ◽  
Author(s):  
Xian-Wei Li ◽  
Yan Liu ◽  
Wei Hao ◽  
Jie-Ren Yang
Keyword(s):  
Diabetic Nephropathy ◽  
Blood Glucose ◽  
High Fat Diet ◽  
Low Dose ◽  
Serum Insulin ◽  
Fasting Blood Glucose ◽  
Diabetic Rats ◽  
High Fat

Sequoyitol decreases blood glucose, improves glucose intolerance, and enhances insulin signaling in ob/ob mice. The aim of this study was to investigate the effects of sequoyitol on diabetic nephropathy in rats with type 2 diabetes mellitus and the mechanism of action. Diabetic rats, induced with a high-fat diet and a low dose of streptozotocin, and were administered sequoyitol (12.5, 25.0, and 50.0 mg·(kg body mass)−1·d−1) for 6 weeks. The levels of fasting blood glucose (FBG), serum insulin, blood urea nitrogen (BUN), and serum creatinine (SCr) were measured. The expression levels of p22phox, p47phox, NF-κB, and TGF-β1 were measured using immunohistochemisty, real-time PCR, and (or) Western blot. The total antioxidative capacity (T-AOC), as well as the levels of malondialdehyde (MDA) and reactive oxygen species (ROS) were also determined. The results showed that sequoyitol significantly decreased FBG, BUN, and SCr levels, and increased the insulin levels in diabetic rats. The level of T-AOC was significantly increased, while ROS and MDA levels and the expression of p22phox, p47phox, NF-κB, and TGF-β1 were decreased with sequoyitol treatment both in vivo and in vitro. These results suggested that sequoyitol ameliorates the progression of diabetic nephropathy in rats, as induced by a high-fat diet and a low dose of streptozotocin, through its glucose-lowering effects, antioxidant activity, and regulation of TGF-β1 expression.

scholarly journals An anxiolytic drug buspirone ameliorates hyperglycemia and endothelial dysfunction in type 2 diabetic rat model

2020 ◽  
Vol 45 (4) ◽  
pp. 397-404
Author(s):  
Tugba Gurpinar Çavuşoğlu ◽  
Ertan Darıverenli ◽  
Kamil Vural ◽  
Nuran Ekerbicer ◽  
Cevval Ulman ◽  
...  
Keyword(s):  
Type 2 Diabetes ◽  
Blood Glucose ◽  
Endothelial Dysfunction ◽  
Vascular Function ◽  
Fasting Blood Glucose ◽  
Diabetic Rats ◽  
Diabetic Rat ◽  
Insulin Levels ◽  
Type 2 Diabetic

AbstractObjectivesType 2 diabetes is a common metabolic disease and anxiety disorders are very common among diabetics. Buspirone is used in the treatment of anxiety, also having blood glucose-lowering effects. The aim of the study was to investigate the effects of buspirone on the glucose and lipid metabolism as well as vascular function in type 2 diabetic rats.MethodsA type 2-diabetic model was induced through a high-fat diet for eight weeks followed by the administration of low-dose streptozotocin (35 mg/kg, intraperitoneal) in rats. Buspirone was given at two different doses (1.5 mg/kg/d and 5 mg/kg/d) and combined with metformin (300 mg/kg/d). The fasting glucose and insulin levels, lipid profile were analyzed, and vascular response measured from the thoracic aorta was also evaluated.ResultsBoth doses of buspirone caused a significant improvement in fasting blood glucose levels. In particular, the buspirone treatment, combined with metformin, improved endothelial dysfunction and was found to be correlated with decreased nitrate/nitrite levels.ConclusionsBuspirone may be effective in the treatment of type 2 diabetes, either alone or in combination with other treatments, particularly in terms of endothelial dysfunction, inflammation and impaired blood glucose, and insulin levels.

scholarly journals ARISTOLOCHIA BRACTEOLATA – ANTIHYPERGLYCEMIC AND ANTIHYPERLIPIDEMIC ACTIVITY IN DEXAMETHASONE-INDUCED DIABETIC RAT MODEL

2017 ◽  
Vol 10 (8) ◽  
pp. 75
Author(s):  
Ganga Rajum ◽  
Hema Sundar Reddy T ◽  
Hema Sundar Reddy T
Keyword(s):  
Blood Glucose ◽  
Methanolic Extract ◽  
Density Lipoprotein ◽  
Diabetic Rats ◽  
Diabetic Rat ◽  
Standard Drug ◽  
Glucose Levels ◽  
Blood Glucose Levels ◽  
Antihyperlipidemic Activity ◽  
Aristolochia Bracteolata

  Objective: The present study was aimed to evaluate antihyperglycemic and antihyperlipidemic activities of methanolic extract of Aristolochia bracteolata (MEAB) against dexamethasone-induced diabetic rat model.Methods: Methanolic extract was prepared by soxhlet extraction and was evaluated for antihyperglycemic and antihyperlipidemic activity using dexamethasone-induced model. The MEAB was administered orally at a dose of 200 and 400 mg/kg body weight glibenclamide was used as standard drug. On 0th and 11th day, blood was collected by retro-orbit plexus.Results: In this model blood glucose levels were determined on 0th and 11th days and MEAB significantly reduced the blood glucose levels in diabetic rats. The effect of MEAB on serum lipid profile such as total cholesterol (TC), triglycerides (TGs), low-density lipoprotein (LDL), very LDL (VLDL), and high-density lipoprotein (HDL) was also measured on the 11th day in the diabetic rats. Significant reduction in TC, TGs, LDL, and VLDL levels and improvement in HDL level were observed in diabetic rats.Conclusion: From the results, it was found that the MEAB possess antihyperglycemic and antihyperlipidemic activities.

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