Enhanced Radiosensitization Effect of Curcumin Delivered by PVP-PCL Nanoparticle in Lung Cancer

Curcumin, the principal polyphenolic curcuminoid, has been reported in numerous studies for its antitumor effect in a series of cancers. It is also reported that curcumin possesses radiosensitization effect in some cancers. However, the poor solubility and unsatisfied bioavailability of curcumin significantly undermine its potential application. Here we prepared curcumin loaded nanoparticles by employing PVP-PCL as drug carrier. Characterization studies indicated the satisfied drug loading efficiency and a sustained in vitro release pattern. Quantification uptake study showed that the uptake efficiency of Cum-NPs by lung cancer cells was time- and dose-dependent. In vitro anticancer study demonstrated the superior cytotoxic effect of Cum-NPs with stronger apoptotic induction over free Cum. Most importantly, there is almost no report on the radiosensitization effect of curcumin loaded nanoparticles. Here, Cum-NPs led to more inhibition of the colony forming ability of A549 cells as compared to the equivalent concentration of free Cum as shown in clonogenic assay. Furthermore, Cum-NPs are much more effective in enhancing the tumor growth inhibitory effect of radiation therapy in a A549 xenograft model. Therefore, results from the current study seem to be the first report on the radiosensitization effect of Cum-NPs and paved the way for a curcumin nanodrug delivery system as a potential radiation adjuvant.

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Cellulose/guar gum hydrogel microspheres as a magnetic anticancer drug carrier

BioResources ◽

10.15376/biores.14.2.3615-3629 ◽

2019 ◽

Vol 14 (2) ◽

pp. 3615-3629 ◽

Cited By ~ 1

Author(s):

Yanli Li ◽

Yucheng Feng ◽

Jun Jing ◽

Fei Yang

Keyword(s):

Anticancer Drug ◽

Guar Gum ◽

Drug Carrier ◽

Drug Loading ◽

In Vitro Release ◽

Adsorption Effect ◽

Magnetic Hydrogel ◽

Vitro Release ◽

Hydrogel Microspheres

A novel magnetic anticancer drug carrier based on cellulose, guar gum, and Fe3O4 hydrogel microspheres was synthesized by chemical crosslinking. These microspheres were crosslinked with epoxy chloropropane and loaded with 5-fluorouracil (5-fu). The effect of the ratio of cellulose to guar gum on bead size, drug loading, and in vitro release behaviors were investigated. The influence of the magnetic content on drug loading and in vitro release behaviors were also evaluated. The magnetic hydrogel microspheres were characterized via an optical microscope, Fourier transform infrared spectroscopy, swelling behavior analysis, vibrating sample magnetometer, and ultraviolet absorption spectroscopy. The results showed that as the ratio of cellulose to guar gum increased from 3:1 to 5:1, the particle size increased from 395 to 459 um. Moreover, the drug loading capacity, encapsulation efficiency, and in vitro release behavior were influenced by the ratio of cellulose/guar gum and Fe3O4 content. Finally, the Fe3O4 particle had an adsorption effect on the drug, thereby reducing the maximum cumulative release.

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Calcium phosphate porous pellets as drug delivery systems: Effect of drug carrier composition on drug loading and in vitro release

Journal of the European Ceramic Society ◽

10.1016/j.jeurceramsoc.2012.01.018 ◽

2012 ◽

Vol 32 (11) ◽

pp. 2679-2690 ◽

Cited By ~ 27

Author(s):

Hiva Baradari ◽

Chantal Damia ◽

Maggy Dutreih-Colas ◽

Etienne Laborde ◽

Nathalie Pécout ◽

...

Keyword(s):

Drug Delivery ◽

Calcium Phosphate ◽

Drug Delivery Systems ◽

Drug Carrier ◽

Drug Loading ◽

In Vitro Release ◽

Delivery Systems ◽

Vitro Release

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5-Fluorouracil-Impregnated PLGA Coated Gold Nanoparticles for Augmented Delivery to Lung Cancer: in vitro Investigations

Anti-Cancer Agents in Medicinal Chemistry ◽

10.2174/1871520622666211224103110 ◽

2021 ◽

Vol 22 ◽

Author(s):

Rashmi Gupta ◽

Leena Vishwakarma ◽

Sunil Kant Guleri ◽

Gourav Kumar

Keyword(s):

Lung Cancer ◽

Gold Nanoparticles ◽

Drug Release ◽

Release Kinetics ◽

Drug Loading ◽

A549 Cells ◽

Double Emulsion ◽

Zero Order Release ◽

Reduced Toxicity

Background and Objective: The study aimed to investigate the augmented cytotoxic effects of polymer-coated (poly-lactic-co-glycolic acid-PLGA) gold nanoparticles (GNPs) carrying 5-fluorouracil (5-FU) in the management of lung cancer. Materials and Methods: In this study, several formulations were prepared using a double emulsion (water-oil-water) method and evaluated for drug release behavior, compatibility, cell line toxicity (A549), and apoptosis assessment. Results: Characterization results showed spherical polydispersed particles with size 29.11-178.21 nm, polydispersity index (PDI) 0.191-292, and zeta potential (ZP) 11.19-29.21 (-mV), respectively. The optimized polymer-coated 5-FU loaded gold nanoparticles (PFGNPs) illustrated a maximum drug loading (93.09 ± 10.75%) compared to others. The percent cumulative drug release of polymer-coated 5-FU loaded nanoparticles (PFNPs), 5-FU loaded gold nanoparticles (FGNPs), (PFGNPs) and 5-FU solution were 47.87± 1.5, 41.09±1.8, 56.31±1.05, and 98.8±4.2%, respectively, over 10 h. following zero-order release kinetics (except 5-FU solution). From the MTT results, the cytotoxic effect of PFGNPs on the A549 cells was 82.89 % compared to the 5-FU solution (74.91 %). EGFR and KRAS gene expression analysis under the influence of PFNPs, FGNPs, PFGNPs, and 5-FU was studied and observed maximum potency for PFNPs. Conclusion: PLGA coated biogenic gold nanoparticles have a combined effect to achieve high drug loading, sustained delivery, improved efficacy, and enhanced permeation. Conclusively, the approach may be promising to control lung cancer with reduced toxicity and improved efficacy.

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The Efficient Apoptotic Induction of Paclitaxel-Loaded Poly(N-vinylpyrrolidone)-block-poly(ε-caprolactone) Nanoparticles in the In Vitro Study of Lung Cancer Cell Lines

Journal of Nanomaterials ◽

10.1155/2015/791063 ◽

2015 ◽

Vol 2015 ◽

pp. 1-8 ◽

Cited By ~ 6

Author(s):

Donghui Zheng ◽

Huiping Ye ◽

Can Luo ◽

Huae Xu ◽

Ling Meng

Keyword(s):

Lung Cancer ◽

Cell Lines ◽

Cancer Cell ◽

In Vitro Release ◽

Drug Carriers ◽

Cancer Cell Lines ◽

Severe Adverse Effect ◽

Lung Cancer Cell ◽

Nanodrug Delivery

Paclitaxel (Ptx) has been established as one of the most important components of first line chemotherapy regimen in the treatment of lung cancer. However, the poor solubility of Ptx makes it employ Cremophor as a solvent, which greatly limits its application due to the severe adverse effect. Encapsulation of Ptx into nanoparticles substantially increases the solubility of Ptx, therefore eradicating the necessity of Cremophor involvement. Here we report on a simple way of preparing Ptx-loaded nanoparticles formed by amphiphilic poly(N-vinylpyrrolidone)-block-poly(ε-caprolactone) (PVP-b-PCL) copolymers. Ptx was incorporated into PVP-b-PCL nanoparticles with a high loading efficiency. In vitro release study shows that Ptx was released from the nanoparticles in a sustained manner. The following experiments including cell staining and cytotoxicity tests indicated that Ptx-NPs led to enhanced induction of apoptosis in non-small-cell lung cancer cell lines NCI-1975 and A549, which is achieved by regulating the expression of apoptosis related proteins. Therefore, data from this study offers an effective way of improving the anticancer efficiency of Ptx by a nanodrug delivery system with amphiphilic PVP-b-PCL as drug carriers.

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Oxymatrine Attenuates Tumor Growth and Deactivates STAT5 Signaling in a Lung Cancer Xenograft Model

Cancers ◽

10.3390/cancers11010049 ◽

2019 ◽

Vol 11 (1) ◽

pp. 49 ◽

Cited By ~ 29

Author(s):

Young Yun Jung ◽

Muthu K. Shanmugam ◽

Acharan S. Narula ◽

Chulwon Kim ◽

Jong Hyun Lee ◽

...

Keyword(s):

Lung Cancer ◽

Cell Proliferation ◽

Mouse Model ◽

A549 Cells ◽

Xenograft Model ◽

Signaling Cascade ◽

Anticancer Effects ◽

Anti Cancer

Oxymatrine (OMT) is a major alkaloid found in radix Sophorae flavescentis extract and has been reported to exhibit various pharmacological activities. We elucidated the detailed molecular mechanism(s) underlying the therapeutic actions of OMT in non-small cell lung cancer (NSCLC) cells and a xenograft mouse model. Because the STAT5 signaling cascade has a significant role in regulating cell proliferation and survival in tumor cells, we hypothesized that OMT may disrupt this signaling cascade to exert its anticancer effects. We found that OMT can inhibit the constitutive activation of STAT5 by suppressing the activation of JAK1/2 and c-Src, nuclear localization, as well as STAT5 binding to DNA in A549 cells and abrogated IL-6-induced STAT5 phosphorylation in H1299 cells. We also report that a sub-optimal concentration of OMT when used in combination with a low dose of paclitaxel produced significant anti-cancer effects by inhibiting cell proliferation and causing substantial apoptosis. In a preclinical lung cancer mouse model, OMT when used in combination with paclitaxel produced a significant reduction in tumor volume. These results suggest that OMT in combination with paclitaxel can cause an attenuation of lung cancer growth both in vitro and in vivo.

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Engineered exosomes loaded with miR-449a selectively inhibit the growth of homologous non-small cell lung cancer

Cancer Cell International ◽

10.1186/s12935-021-02157-7 ◽

2021 ◽

Vol 21 (1) ◽

Author(s):

Wen Zhou ◽

Mingming Xu ◽

Zhipeng Wang ◽

Mingjun Yang

Keyword(s):

Lung Cancer ◽

Small Cell Lung Cancer ◽

Cell Lung Cancer ◽

Transfection Efficiency ◽

Drug Carrier ◽

A549 Cells ◽

Small Cell ◽

Small Cell Lung

AbstractAs an efficient drug carrier, exosome has been widely used in the delivery of genetic drugs, chemotherapeutic drugs, and anti-inflammatory drugs. As a genetic drug carrier, exosomes are beneficial to improve transfection efficiency and weaken side effects at the same time. Here, we use genetic engineering to prepare engineered exosomes (miR-449a Exo) that can actively deliver miR-449a. It was verified that miR-449a Exo had good homology targeting capacity and was specifically taken up by A549 cells. Moreover, miR-449a Exo had high delivery efficiency of miR-449a in vitro and in vivo. We demonstrated that miR-449a Exo effectively inhibited the proliferation of A549 cells and promoted their apoptosis. In addition, miR-449a Exo was found to control the progression of mouse tumors and prolong their survival in vivo. Our research provides new ideas for exosomes to efficiently and actively load gene drugs, and finds promising methods for the treatment of non-small cell lung cancer.

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Inhibitory Effect of pH-Responsive Nanogel Encapsulating Ginsenoside CK against Lung Cancer

Polymers ◽

10.3390/polym13111784 ◽

2021 ◽

Vol 13 (11) ◽

pp. 1784

Author(s):

Ziyang Xue ◽

Rongzhan Fu ◽

Zhiguang Duan ◽

Lei Chi ◽

Chenhui Zhu ◽

...

Keyword(s):

Lung Cancer ◽

Water Solubility ◽

Drug Loading ◽

Survival Rates ◽

A549 Cells ◽

Ph Responsive ◽

Antitumor Effects ◽

Hydrophobic Cavity

Ginsenoside CK is one of the intestinal bacterial metabolites of ginsenoside prototype saponins, such as ginsenoside Rb1, Rb2, Rc, and Rd. Poor water solubility and low bioavailability have limited its application. The nanogel carriers could specifically deliver hydrophobic drugs to cancer cells. Therefore, in this study, a nanogel was constructed by the formation of Schiff base bonds between hydrazide-modified carboxymethyl cellulose (CMC-NH2) and aldehyde-modified β-cyclodextrin (β-CD-CHO). A water-in-oil reverse microemulsion method was utilized to encapsulate ginsenoside CK via the hydrophobic cavity of β-CD. β-CD-CHO with a unique hydrophobic cavity carried out efficient encapsulation of CK, and the drug loading and encapsulation efficiency were 16.4% and 70.9%, respectively. The drug release of CK-loaded nanogels (CK-Ngs) in vitro was investigated in different pH environments, and the results showed that the cumulative release rate at pH 5.8 was 85.5% after 140 h. The methylthiazolyldiphenyl-tetrazolium bromide (MTT) toxicity analysis indicated that the survival rates of A549 cells in CK-Ngs at 96 h was 2.98% compared to that of CK (11.34%). In vivo animal experiments exhibited that the inhibitory rates of CK-Ngs against tumor volume was 73.8%, which was higher than that of CK (66.1%). Collectively, the pH-responsive nanogel prepared herein could be considered as a potential nanocarrier for CK to improve its antitumor effects against lung cancer.

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Facile Preparation of Paclitaxel Nano-Suspensions to Treat Lung Cancer

Journal of Biomaterials and Tissue Engineering ◽

10.1166/jbt.2022.2940 ◽

2022 ◽

Vol 12 (4) ◽

pp. 690-694

Author(s):

Wei Zhang ◽

Yi Chen ◽

Bin Wang ◽

Xueren Feng ◽

Lijuan Zhang ◽

...

Keyword(s):

Lung Cancer ◽

Drug Loading ◽

A549 Cells ◽

Pharmaceutical Formulations ◽

First Line ◽

Facile Preparation ◽

Tumor Accumulation ◽

Line Chemotherapy

Lung cancer is a worldwide issue which account for the death of thousands every year. Paclitaxel (PTX) as the first line chemotherapy drug to treat lung cancer, its clinical applications is largely limited by its poor solubility. The facile preparation of pharmaceutical formulations to increase the solubility as well as targetability of PTX is of vital importance in lung cancer treatment. Herein, we introduced a facile method to prepare PTX nano-suspensions (NSs), which have high drug loading as well as well-dispersed particle size. The in vitro cell experiments revealed its capability to enhance the drug accumulation in A549 cells than free PTX. Moreover, in vivo animal assay suggested its better tumor accumulation and antitumor efficacy than PTX injection (Taxol).

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Preparation of Chitosan/Cyclodextrin/Trisodium Citrate Nanoparticles for the Poor-Water Drug Carrier Material

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.152-153.1356 ◽

2010 ◽

Vol 152-153 ◽

pp. 1356-1359 ◽

Cited By ~ 1

Author(s):

Jin Gou Ji ◽

Shi Lei Hao ◽

Jin Dong ◽

Jing Fen Zhang ◽

Jing Jie Li ◽

...

Keyword(s):

Zeta Potential ◽

Drug Carrier ◽

Drug Loading ◽

In Vitro Release ◽

Trisodium Citrate ◽

Loading Capacity ◽

The Poor ◽

Release Studies ◽

Average Size

The ibuprofen (IBU) was selected as a model of the poor-water drugs, and the IBU-loaded chitosan/β-cyclodextrin/trisodium citrate nanoparticles were prepared via the ionic cross-linking method. The drug-loading capacity, particle size, zeta potential and surface morphology of the obtained nanoparticles were characterized. The results showed that the IBU had been successfully loaded into the nanoparticles, and showed good drug-loading capacity. The prepared nanoparticles were spherical morphology with an average size of 293.7 nm and a Zeta potential of +30.72 mV. The in vitro release studies showed that the controlled release of IBU from the nanoparticles was followed.

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