Preventive Effect of the Herbal Preparation, HemoHIM, on Cisplatin-Induced Immune Suppression

We determined the functional effect of the herbal preparation, HemoHIM, on the immune system, by examining the immunomodulatory activities of HemoHIM using immunocompromised mice. In this study, to examine the effect on the restoration of immune cells and balance in the immune system, we utilized a cisplatin-induced immunosuppression mouse model. Mice were injected intraperitoneally with cisplatin, an immunosuppressive anticancer, and then received oral doses of 100, 250, and 500 mg/kg of HemoHIM for 14 days. The HemoHIM prevented the cisplatin-induced loss of body and organ weight. In terms of innate immunity, natural killer (NK) cell activity and phagocytosis increased in the HemoHIM group compared to the cisplatin control group. The HemoHIM group also showed a significantly higher expression of Th1-mediated cytokines (interferon gamma (IFN-γ), interleukin-2 (IL-2), and tumor necrosis factor alpha (TNF-α)) and inhibited the production of Th2-mediated cytokine interleukin-4 (IL-4) compared to cisplatin control group. These findings indicate that HemoHIM enhances immune activity by modulating immune cell activity and cytokine secretion in immune-suppressed mice.

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Nintedanib reduces corticosteroid resistance pulmonary fibrosis induced by bleomycin in mice by increasing the expression of β3 & β6 integrins

10.21203/rs.3.rs-899285/v1 ◽

2021 ◽

Author(s):

Doha O Alghamdi ◽

Hala S Abdel Kawy ◽

Zuhair A Damanhouri

Keyword(s):

Pulmonary Fibrosis ◽

Lung Tumor ◽

Histopathological Examination ◽

Interleukin 2 ◽

Interleukin 4 ◽

Control Group ◽

Lung Water ◽

Necrosis Factor Alpha ◽

Major Health Problem ◽

Corticosteroid Resistance

Abstract Background:Corticosteroid resistance pulmonary fibrosis is a major health problem. This study aimed to determine the effectiveness of nintedanib on corticosteroid resistance pulmonary fibrosis induced by bleomycin in mice.Methods:The mice were divided into five groups 12 mice each. control group, BLM group received single dose of bleomycin (BLM), BLM+MP group received BLM and methylprednisolone (MP), BLM+NIN group received BLM and nintedanib(NIN) and BLM + NIN + MP group. The lung tissues were obtained for biochemical analysis, gene expression and histopathological examination on day 7 and day 28.Results:After 7 days, both NIN groups showed a significant decrease in the levels of interleukin-2, interleukin-4, interferon-gamma, lung tumor necrosis factor-alpha, Malondialdehyde and lung water content with a significant increase in the Glutathione level in lung tissues compared to MP group. After 28 days, both NIN groups showed a significant reduction in hydroxyproline, and Trans-forming Growth Factor beta lung tissues contents compared to MP group, and they showed a positive effect on the expression of β 3 &β6 integrins compared to the negative effect of MP group. Histopathologically, both NIN groups showed significant improvement compared to MP group by H&E and Masson’s trichrome stains. Immunohistochemical staining revealed negative BCL-2 expression in the cytoplasm of bronchiolar epithelium in both NIN groups after 7 and 28 days of treatment. Lung tissue morphometric studies showed significant improvement of pathological changes induced by BLM in both NIN groups.Conclusion:Altogether, our data indicates that nintedanib overcame corticosteroid resistance pulmonary fibrosis induced by bleomycin.

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Impairment of Intramacrophagic Brucella suis Multiplication by Human Natural Killer Cells through a Contact-Dependent Mechanism

Infection and Immunity ◽

10.1128/iai.72.4.2303-2311.2004 ◽

2004 ◽

Vol 72 (4) ◽

pp. 2303-2311 ◽

Cited By ~ 13

Author(s):

Jacques Dornand ◽

Virginie Lafont ◽

Jane Oliaro ◽

Annie Terraza ◽

Elsa Castaneda-Roldan ◽

...

Keyword(s):

Nk Cells ◽

Nk Cell ◽

Interleukin 2 ◽

Cell Activity ◽

Human Brucellosis ◽

Cell Contact ◽

Necrosis Factor Alpha ◽

Brucella Suis ◽

Human Natural Killer Cells

ABSTRACT Brucella spp. are facultative intracellular bacteria that can establish themselves and cause chronic disease in humans and animals. NK cells play a key role in host defense. They are implicated in an early immune response to a variety of pathogens. However, it was shown that they do not control Brucella infection in mice. On the other hand, NK cell activity is impaired in patients with acute brucellosis, and recently it was demonstrated that human NK cells mediate the killing of intramacrophagic Mycobacterium tuberculosis in in vitro infection. Therefore, we have analyzed the behavior of Brucella suis infecting isolated human macrophages in the presence of syngeneic NK cells. We show that (i) NK cells impair the intramacrophagic development of B. suis, a phenomenon enhanced by NK cell activators, such as interleukin-2; (ii) NK cells cultured in the presence of infected macrophages are highly activated and secrete gamma interferon and tumor necrosis factor alpha; (iii) impairment of bacterial multiplication inside infected cells is marginally associated with the cytokines produced during the early phase of macrophage-NK cell cocultures; (iv) direct cell-to-cell contact is required for NK cells to mediate the inhibition of B. suis development; and (v) inhibition of B. suis development results from an induction of NK cell cytotoxicity against infected macrophages. Altogether, these findings show that NK cells could participate early in controlling the intramacrophagic development of B. suis in humans. It seems thus reasonable to hypothesize a role for NK cells in the control of human brucellosis. However, by impairing the activity of these cells in the acute phase of the illness, the pathogen should avoid this control.

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Nintedanib reduces corticosteroid resistance pulmonary fibrosis induced by bleomycin in mice by increasing the expression of β3 & β6 integrins

10.21203/rs.3.rs-898520/v1 ◽

2021 ◽

Author(s):

doha omar alghamdi ◽

Hala S Abdel kawy ◽

Zhair A Damanhouri

Keyword(s):

Pulmonary Fibrosis ◽

Lung Tumor ◽

Histopathological Examination ◽

Interleukin 2 ◽

Interleukin 4 ◽

Control Group ◽

Lung Water ◽

Necrosis Factor Alpha ◽

Major Health Problem ◽

Corticosteroid Resistance

Abstract Background: Corticosteroid resistance pulmonary fibrosis is a major health problem. This study aimed to determine the effectiveness of nintedanib on corticosteroid resistance pulmonary fibrosis induced by bleomycin in mice. Methods: The mice were divided into five groups 12 mice each. control group, BLM group received single dose of bleomycin (BLM), BLM+MP group received BLM and methylprednisolone (MP), BLM+NIN group received BLM and nintedanib(NIN) and BLM + NIN + MP group. The lung tissues were obtained for biochemical analysis, gene expression and histopathological examination on day 7 and day 28. Results: after 7 days, both NIN groups showed a significant decrease in the levels of interleukin-2, interleukin-4, interferon-gamma, lung tumor necrosis factor-alpha, Malondialdehyde and lung water content with a significant increase in the Glutathione level in lung tissues compared to MP group. After 28 days, both NIN groups showed a significant reduction in hydroxyproline, and Trans-forming Growth Factor beta lung tissues contents compared to MP group, and they showed a positive effect on the expression of β 3 &β6 integrins compared to the negative effect of MP group. Histopathologically, both NIN groups showed significant improvement compared to MP group by H&E and Masson’s trichrome stains. Immunohistochemical staining revealed negative BCL-2 expression in the cytoplasm of bronchiolar epithelium in both NIN groups after 7 and 28 days of treatment. Lung tissue morphometric studies showed significant improvement of pathological changes induced by BLM in both NIN groups. Conclusion: Altogether, our data indicates that nintedanib overcame corticosteroid resistance pulmonary fibrosis induced by bleomycin.

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Influence of in vivo hypobaric hypoxia on function of lymphocytes, neutrocytes, natural killer cells, and cytokines

Journal of Applied Physiology ◽

10.1152/jappl.1993.74.3.1100 ◽

1993 ◽

Vol 74 (3) ◽

pp. 1100-1106 ◽

Cited By ~ 41

Author(s):

M. Klokker ◽

A. Kharazmi ◽

H. Galbo ◽

I. Bygbjerg ◽

B. K. Pedersen

Keyword(s):

Immune System ◽

Nk Cells ◽

Natural Killer ◽

Nk Cell ◽

Interleukin 2 ◽

Cell Activity ◽

Nk Cell Activity ◽

Cd8 Cells ◽

The Absolute

We have investigated the effects of short-term hypoxia in vivo on the human cellular immune system. Seven young healthy volunteers were placed in a decompression chamber (380 Torr) for 20 min with or without supplemental O2. The leukocyte concentration increased during hypobaric conditions because of an increased concentration of lymphocytes. The absolute and relative concentration of CD16+ natural killer (NK) cells increased markedly during hypoxia and returned to pretest values after 2 h of recovery. The NK cell activity of blood mononuclear cells (BMNC, %lysis/fixed no. of BMNC) boosted with interferon-alpha, interleukin-2 (IL-2), and indomethacin rose in parallel with unboosted NK cell activity during hypoxia. The percentage of CD4+ and CD8+ cells declined during hypoxia, whereas the absolute concentration of both CD8+ cells and CD14+ monocytes increased. Although the BMNC composition varied, the proliferative responses of BMNC after stimulation with phytohemagglutinin, purified derivative of tuberculin, and IL-2 did not change significantly. The in vitro production of interleukin-1 beta and IL-2 in supernatants obtained after stimulation of BMNC with phytohemagglutinin or lipopolysaccharide was not affected. The chemiluminescence response of neutrocytes increased 2 h after hypoxia. It was concluded that acute hypoxia induced marked alterations in the immune system and that the NK cells are especially sensitive to the hypoxic stimulus.

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Effect of Pleuran (β-glucan from Pleurotus ostreatus) supplementation on cellular immune response after intensive exercise in elite athletes

Applied Physiology Nutrition and Metabolism ◽

10.1139/h10-070 ◽

2010 ◽

Vol 35 (6) ◽

pp. 755-762 ◽

Cited By ~ 35

Author(s):

Marián Bobovčák ◽

Renata Kuniaková ◽

Ján Gabriž ◽

Juraj Majtán

Keyword(s):

Immune System ◽

Pleurotus Ostreatus ◽

Immune Cell ◽

Nk Cell ◽

Elite Athletes ◽

Recovery Period ◽

Cell Activity ◽

Positive Effects ◽

Cell Counts ◽

Intensive Exercise

Excessive and exhausting physical loads depress the immune system. Carbohydrate consumption may minimize the postexercise suppression of the innate immune system. β-Glucan is a well-known immunomodulator, with positive effects on the functioning of immunocompetent cells. The goal of this study was to determine whether β-glucan dietary supplementation from the mushroom Pleurotus ostreatus decreases the suppressed immune system responses induced by short-term high-intensity exercise in humans. In this double-blind pilot study, 20 elite athletes were randomized to β-glucan (n = 9) or placebo (n = 11) groups; these groups consumed 100 mg of β-glucan (Imunoglukan) or placebo supplements, respectively, once a day for 2 months. Venous whole blood was collected before and after 2 months of supplementation (baseline), both immediately and 1 h after (recovery period) a 20-min intensive exercise bout at the end of the supplementation period. The blood samples were used to measure the cell counts of leukocytes, erythrocyte, and lymphocytes; subpopulations of lymphocytes, granulocytes, and monocytes; and natural killer (NK) cell activity (NKCA). A 28% reduction in NKCA (p < 0.01) below the baseline value was observed in the placebo group during the recovery period, whereas no significant reduction in NKCA was found in the β-glucan group. In addition, no significant decrease in NK cell count was measured in the β-glucan group during the recovery period. Immune cell counts did not differ significantly between the groups. These results indicate that insoluble β-glucan supplementation from P. ostreatus may play a role in modulating exercise-induced changes in NKCA in intensively training athletes.

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Nintedanib reduces corticosteroid resistance pulmonary fibrosis induced by bleomycin in mice by increasing the expression of β3 & β6 integrins

10.21203/rs.3.rs-899285/v2 ◽

2021 ◽

Author(s):

doha omar alghamdi ◽

Hala S Abdel Kawy ◽

zuhair A Damanhouri

Keyword(s):

Pulmonary Fibrosis ◽

Lung Tumor ◽

Histopathological Examination ◽

Interleukin 2 ◽

Interleukin 4 ◽

Control Group ◽

Lung Water ◽

Necrosis Factor Alpha ◽

Major Health Problem ◽

Corticosteroid Resistance

Abstract Background: Corticosteroid resistance pulmonary fibrosis is a major health problem. This study aimed to determine the effectiveness of nintedanib on corticosteroid resistance pulmonary fibrosis induced by bleomycin in mice. Methods: The mice were divided into five groups 12 mice each. control group, BLM group received single dose of bleomycin (BLM), BLM+MP group received BLM and methylprednisolone (MP), BLM+NIN group received BLM and nintedanib(NIN) and BLM + NIN + MP group. The lung tissues were obtained for biochemical analysis, gene expression and histopathological examination on day 7 and day 28. Results: after 7 days, both NIN groups showed a significant decrease in the levels of interleukin-2, interleukin-4, interferon-gamma, lung tumor necrosis factor-alpha, Malondialdehyde and lung water content with a significant increase in the Glutathione level in lung tissues compared to MP group. After 28 days, both NIN groups showed a significant reduction in hydroxyproline, and Trans-forming Growth Factor beta lung tissues contents compared to MP group, and they showed a positive effect on the expression of β 3 &β6 integrins compared to the negative effect of MP group. Histopathologically, both NIN groups showed significant improvement compared to MP group by H&E and Masson’s trichrome stains. Immunohistochemical staining revealed negative BCL-2 expression in the cytoplasm of bronchiolar epithelium in both NIN groups after 7 and 28 days of treatment. Lung tissue morphometric studies showed significant improvement of pathological changes induced by BLM in both NIN groups. Conclusions: Altogether, our data indicates that nintedanib overcame corticosteroid resistance pulmonary fibrosis induced by bleomycin.

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Construction of Recombinant Lactococcus lactis Strain Expressing VP1 Fusion Protein of Duck Hepatitis A Virus Type 1 and Evaluation of Its Immune Effect

Vaccines ◽

10.3390/vaccines9121479 ◽

2021 ◽

Vol 9 (12) ◽

pp. 1479

Author(s):

Xiaoting Zhang ◽

Ruihua Zhang ◽

Jingyu Wang ◽

Nana Sui ◽

Guige Xu ◽

...

Keyword(s):

Immune System ◽

Fusion Protein ◽

Lactococcus Lactis ◽

Virus Type ◽

Hepatitis A ◽

Hepatitis A Virus ◽

Interleukin 2 ◽

Interleukin 4 ◽

Duck Hepatitis

With the continuous development of duck farming and the increasing breeding density, the incidence of duck hepatitis A virus type 1 (DHAV-1) has been on the rise, seriously endangering the development of duck farming. To reduce the use of antibiotics in duck breeding, susceptibility risks and mortality, and avoid virulence recovery and immune failure risk, this study aims to develop a new type of mucosal immune probiotics and make full use of molecular biology techniques, on the level of genetic engineering, to modify Lactococcus lactis (L. lactis). In this study, a secretory recombinant L. lactis named MG1363-VP1 with an enhanced Green Fluorescent Protein (eGFP) and translation enhancer T7g10L was constructed, which could express the VP1-eGFP fusion protein of DHAV-1. The animal experiment in ducklings was performed to detect the immune response and protection effect of oral microecologics by recombinant L. lactis. The results showed that oral L. lactis MG1363-VP1 significantly induced the body’s humoral immune system and mucosal immune system to produce specific anti-VP1 IgG antibodies and mucosal secretory immunoglobulin A (sIgA) for DHAV-1 in ducklings, and cytokines including interleukin-2 (IL-2), interleukin-4 (IL-4), interleukin-10 (IL-10), and interferon gamma (IFN-γ). The mortality rate was monitored simultaneously by the natural infestation in the process of production and breeding; notably, the ducklings vaccinated with L. lactis MG1363-VP1 were effectively protected against the nature infection of DHAV-1. The recombinant L. lactis MG1363-VP1 constructed in this study provides a new means of preventing and controlling DHAV-1 infection in the future.

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Immunomodulatory Effects of Perioperative Dexmedetomidine in Ovarian Cancer: An In Vitro and Xenograft Mouse Model Study

Frontiers in Oncology ◽

10.3389/fonc.2021.722743 ◽

2021 ◽

Vol 11 ◽

Author(s):

Seokyung Shin ◽

Ki Jun Kim ◽

Hye Jeong Hwang ◽

Sewon Noh ◽

Ju Eun Oh ◽

...

Keyword(s):

Cell Cycle ◽

Ovarian Cancer ◽

Mouse Model ◽

Cell Viability ◽

Nk Cell ◽

Cell Activity ◽

Tumor Burden ◽

Control Group ◽

Nk Cell Activity ◽

Dexmedetomidine Infusion

BackgroundThe surgical stress response (SSR) causes immunosuppression which may cause residual tumor growth and micrometastasis after cancer surgery. We investigated whether dexmedetomidine affects cancer cell behavior and immune function in an ovarian cancer xenograft mouse model.MethodsThe effect of dexmedetomidine on cell viability and cell cycle was assessed using SK-OV-3 cells at drug concentrations of 0.5, 0.1, 5, and 10 µg mL-1. BALB/c nude mice were used for the ovarian cancer model with the Dexmedetomidine group (n=6) undergoing surgery with dexmedetomidine infusion and the Control group (n=6) with saline infusion for 4 weeks. Natural killer (NK) cell activity, serum proinflammatory cytokines, and cortisol were measured at predetermined time points and tumor burden was assessed 4 weeks after surgery.ResultsDexmedetomidine had no effect on cell viability or cell cycle. Following a sharp decrease on postoperative day (POD) 1, NK cell activity recovered faster in the Dexmedetomidine group with significant difference vs. the Control group on POD 3 (P=0.028). In the Dexmedetomidine group, cortisol levels were lower on POD 3 (P=0.004) and TNF-α levels were lower at 4 weeks after surgery (P<0.001) compared to the Control group. The Dexmedetomidine group showed lower tumor burden at 4 weeks vs. the Control group as observed by both tumor weight (P<0.001) and the in vivo imaging system (P=0.03).ConclusionsDexmedetomidine infusion may improve ovarian cancer surgery outcome by suppressing the SSR and stress mediator release. Further studies are needed to elucidate the mechanisms by which dexmedetomidine acts on cancer and immune cells.

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Analysis of the Effect of Psychological Nursing Intervention on Perioperative Mood and NK Cell Activity in Breast Cancer Patients

Tobacco Regulatory Science ◽

10.18001/trs.7.5.1.104 ◽

2021 ◽

Vol 7 (5) ◽

pp. 3301-3314

Author(s):

Jihong Yuan ◽

Linghong Yuan ◽

Yi Hu ◽

Jingjing Gong ◽

Yang Tong ◽

...

Keyword(s):

Breast Cancer ◽

Cancer Patients ◽

Nk Cell ◽

Psychological Intervention ◽

Cell Activity ◽

Nursing Intervention ◽

Control Group ◽

Breast Cancer Patients ◽

Nk Cell Activity ◽

Intervention Measures

Breast cancer (Breast Cancer, BC) is one of the most common malignant tumors in women in the world. It is the first malignant tumor that causes the death of women in developing countries. It seriously threatens the lives and health of women and causes damage to the family, economy and society. Through psychological nursing intervention, it has a positive effect on the perioperative mood and NK cell activity of breast cancer patients. The purpose of this article is to explore the effects of psychological intervention on the perioperative mood and NK cell activity of breast cancer patients. This article is based on the concept and theory of psychological nursing intervention for female breast cancer patients, and establishes personalized intervention measures in conjunction with clinical practice to intervene the emotions of breast cancer patients during the perioperative period. This article analyzes the nature, intensity, and causes of negative psychology of the subject through in-depth understanding of the subject’s psychological emotions, coping ability, personality characteristics, past emotional experience, and social support, and discovers the different emotional characteristics of the patient, and formulates the personality psychological intervention measures to stimulate positive and optimistic attitudes and ease the degree of negative psychology. This article analyzes the psychological characteristics of several breast cancer patients. Take corresponding psychological intervention measures. Cope with a series of bad psychology caused by image damage caused by total mastectomy. The results and data in this article show that the incidence of anxiety and depression in the control group is higher than that of the psychological intervention group at 7 days after the operation, with P values of 0.0059 and 0. 0215.Psychological intervention reduces the incidence of negative emotions and reduces the negativeness of patients. The intensity of emotion has played a good clinical effect.

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Renal cell carcinoma: treatment with recombinant interleukin-2 plus beta-interferon.

Journal of Clinical Oncology ◽

10.1200/jco.1990.8.3.460 ◽

1990 ◽

Vol 8 (3) ◽

pp. 460-467 ◽

Cited By ~ 42

Author(s):

R L Krigel ◽

K A Padavic-Shaller ◽

A R Rudolph ◽

M Konrad ◽

E C Bradley ◽

...

Keyword(s):

Renal Cell Carcinoma ◽

Local Recurrence ◽

Cell Carcinoma ◽

Renal Cell ◽

Nk Cell ◽

Objective Response ◽

Interleukin 2 ◽

Cell Activity ◽

Nk Cell Activity ◽

Beta Interferon

Preclinical data have demonstrated synergy between interleukin-2 (IL-2) and beta-interferon (IFN-beta) in stimulating natural-killer (NK) cell activity and in increasing expression of IL-2 receptors. Based on results of a phase I trial, a combination of IL-2 and IFN-beta was administered three times weekly by intravenous (IV) bolus injection with 5 x 10(6) Cetus U/m2 of IL-2 and 6 x 10(6) U/m2 of IFN-beta to 24 patients with advanced renal cell carcinoma (RCC). Of 22 assessable patients there were six (27%) objective responses including one complete remission (CR) and five partial responses (PRs). There were three minor responses (MRs), 11 stable disease (SD), and two progressive disease (PD). Two of the objective responses have continued for almost 2 years. Response sites include lymph nodes, lungs, and bone. Toxicities requiring dose reduction include arthralgia, weight loss, fatigue, decreased performance status, depression, and hypotension. Five of 10 patients who had a prior nephrectomy without local recurrence achieved an objective response as compared with only one of 12 without a prior nephrectomy or with a local recurrence (P = .04). Mean peak lymphokine-activated killer (LAK) cell activity of the objective responders was 88 lytic units (LU) as compared with 4 LU in the nonresponders (P = .01). Mean peak NK cell activity was 288 LU in the objective responders as compared with 100 LU in the nonresponders (P = .10).(ABSTRACT TRUNCATED AT 250 WORDS)

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