scholarly journals Antiproliferative Activities of Methanolic Extract and Fractions of Tetrapleura Tetraptera Fruit

2021 ◽  
Vol 2021 ◽  
pp. 1-11
Author(s):  
Anastasia Rosebud Aikins ◽  
Peggy Afua Birikorang ◽  
Mary Chama ◽  
Eunice Dotse ◽  
Abigail Anning ◽  
...  
Keyword(s):  
Anticancer Agents ◽  
Methanolic Extract ◽  
Colorimetric Assay ◽  
Jurkat Cells ◽  
Mass Spectrometry Analysis ◽  
Spectrometry Analysis ◽  
Cancer Chemotherapeutics ◽  
Tetrapleura Tetraptera ◽  
Antiproliferative Activities ◽  
Mcf 7

Most of the current cancer chemotherapeutics are associated with harsh and undesirable side effects, including toxicity and chemoresistance, driving the need for safer and more effective alternatives. In this study, the antiproliferative activities of the methanolic extract of Tetrapleura tetraptera fruits and nine different fractions (C1–C9) from the column chromatographic separation of the extract against leukemia (Jurkat) and human breast cancer (MCF-7) cell lines were investigated using a tetrazolium-based colorimetric assay. Phytochemical screening of the extract and fractions found alkaloids, carbohydrates, flavonoids, glycosides, phenols, saponins, steroids, tannins, and terpenoids in the methanolic extract. Most of the fractions exhibited antiproliferative activity (>100 μg/mL) with the Jurkat cells being more susceptible than the MCF-7 cells. Four of the collected fractions C4, C3, C5, and C2 had good selective indices in decreasing order of activity, in the case of Jurkat cells. Liquid chromatography-mass spectrometry analysis of all samples (except for C4 and C9) revealed that C1, C2, C3, and C5 each had a single component. More importantly, fractions C2, C3, and C5, which were selective to Jurkat cells, also had the same retention time of 1.846 min. Fractions C6 and C8 had two components, with C7 having four components. This study serves as a basis for further work to isolate and characterize potential anticancer agents from the fractions of extracts of T. tetraptera fruits.

scholarly journals ENIGMATIC INDUCTION OF CYTOMIXIS IN ALLIUM CEPA ROOT MERISTEM BY AGLAIA EDULIS ROXB. LEAF EXTRACT AND ITS PHYTOCHEMICAL RATIONALE

2020 ◽  
pp. 168-171
Author(s):  
ARCHANA ELAMKULAM RAVINDRAN ◽  
JOHN ERNEST THOPPIL
Keyword(s):  
Allium Cepa ◽  
Leaf Extract ◽  
Root Meristem ◽  
Methanolic Extract ◽  
Synergistic Action ◽  
Mass Spectrometry Analysis ◽  
Gas Chromatography Mass Spectrometry ◽  
Phytochemical Analysis ◽  
Spectrometry Analysis ◽  
Dose Dependent

Objective: The present study aims to analyze the potential of Aglaia edulis Roxb. leaf extract to induce cytological aberrations in Allium cepa root meristem and to determine the phytoconstituents in the extract. Methods: Cytotoxicity evaluation of the leaf methanolic extract was done using Allium cepa assay using various concentrations. Volatile phytoconstituents in the extract were determined using gas chromatography–mass spectrometry analysis. Results: Considerable number of cytomictic cells along with other aberrations was observed. The occurrence of cytomixis was found to be dose dependent where it ranged from 6.58±0.35 to 29.45±0.45. The percentage of cytomictic cells among the total aberrant cells was observed between 35.19±1.67 and 77.39±1.39. The phytochemical analysis of the plant extract revealed the presence of active secondary metabolites. Conclusion: The synergistic action of the active compounds might have triggered the phenomenon of cytomixis which, in turn, could be exploited for the production of polyploids.

Anthonotha macrophylla P. Beauv (Caesalpiniaceae) aqueous extract exhibits antiestrogenic effects in vitro and in vivo

2019 ◽  
Vol 0 (0) ◽  
Author(s):  
Telesphore Nanbo Gueyo ◽  
Marie Alfrede Mvondo ◽  
Stéphane Zingue ◽  
Marius Trésor Kemegne Sipping ◽  
Larissa Vanelle Kenmogne ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Aqueous Extract ◽  
Mass Spectrometry Analysis ◽  
Spectrometry Analysis ◽  
Flight Mass Spectrometry ◽  
Estrogen Receptor Modulators ◽  
Mcf 7 ◽  
Antiestrogenic Effects

AbstractBackgroundPhytoestrogens are natural compounds known as natural selective estrogen receptor modulators used as alternatives against estrogen-dependent cancers. This study aims to evaluate the antiestrogenic effects of Anthonotha macrophylla, a plant used to treat cancer in Cameroon.MethodsThe estrogenic/antiestrogenic activities of A. macrophylla aqueous extract were evaluated in vitro using MCF-7 cell proliferation assay. Moreover, a classical uterotrophic test was carried out to evaluate the antiestrogenic effects of A. macrophylla in rats. Changes in the uterus, vagina, and mammary glands were used as endpoints of estrogenicity.ResultsAnthonotha macrophylla induced antiestrogenic effects in vitro at all the tested concentrations by inhibiting estradiol-induced MCF-7 cell proliferation (p < 0.001). In vivo, a coadministration of estradiol with A. macrophylla extract led to the decrease of uterine [150 (p < 0.05) and 300 (p < 0.01) mg/kg body weight (BW)] and vaginal [75 (p < 0.01) and 300 (p < 0.05) mg/kg BW] epithelial thickness. In addition, a reduction in the mammary gland acini lumen’s diameter was also observed at 75 and 150 mg/kg. Gas chromatography-time-of-flight-mass spectrometry analysis showed that phenolic acid derivatives are present in A. macrophylla extract, which are well known to be endowed with estrogenic/antiestrogenic properties. The LD50 of A. macrophylla was estimated to be less than 2000 mg/kg.ConclusionsAnthonotha macrophylla aqueous extract has antiestrogenic properties. This could promote more studies to explore its ability to prevent estrogen-dependent cancers.

scholarly journals Optimization of Formaldehyde Cross-Linking for Protein Interaction Analysis of Non-Tagged Integrinβ1

2010 ◽  
Vol 2010 ◽  
pp. 1-13 ◽  
Author(s):  
Cordula Klockenbusch ◽  
Juergen Kast
Keyword(s):  
Mass Spectrometry ◽  
Protein Interactions ◽  
Interaction Analysis ◽  
Protein Complexes ◽  
Human Platelets ◽  
Jurkat Cells ◽  
Mass Spectrometry Analysis ◽  
Cross Linking ◽  
High Molecular Weight Complex ◽  
Spectrometry Analysis

Formaldehyde cross-linking of protein complexes combined with immunoprecipitation and mass spectrometry analysis is a promising technique for analysing protein-protein interactions, including those of transient nature. Here we used integrinβ1 as a model to describe the application of formaldehyde cross-linking in detail, particularly focusing on the optimal parameters for cross-linking, the detection of formaldehyde cross-linked complexes, the utility of antibodies, and the identification of binding partners. Integrinβ1 was found in a high molecular weight complex after formaldehyde cross-linking. Eight different anti-integrinβ1 antibodies were used for pull-down experiments and no loss in precipitation efficiency after cross-linking was observed. However, two of the antibodies could not precipitate the complex, probably due to hidden epitopes. Formaldehyde cross-linked complexes, precipitated from Jurkat cells or human platelets and analyzed by mass spectrometry, were found to be composed of integrinβ1,α4 andα6 orβ1,α6,α2, andα5, respectively.

New Benzaldehyde and Benzopyran Compounds from the Endophytic Fungus Paraphaeosphaeria sp. F03 and Their Antimicrobial and Cytotoxic Activities

Planta Medica ◽  
2019 ◽  
Vol 85 (11/12) ◽  
pp. 957-964 ◽  
Author(s):  
Marcelo R. de Amorim ◽  
Felipe Hilário ◽  
Fernando M. dos Santos ◽  
João M. Batista ◽  
Tais M. Bauab ◽  
...  
Keyword(s):  
Endophytic Fungus ◽  
Inhibitory Concentration ◽  
High Resolution Mass Spectrometry ◽  
Mass Spectrometry Analysis ◽  
Racemic Mixture ◽  
Cytotoxic Activities ◽  
Spectrometry Analysis ◽  
Ic50 Values ◽  
Benzaldehyde Derivatives ◽  
Mcf 7

AbstractThree new benzaldehyde derivatives, sporulosaldeins A – C (1–3), and 3 new benzopyran derivatives, sporulosaldeins D – F (4–6), were discovered from an endophytic fungus, Paraphaeosphaeria sp. F03, which was isolated from Paepalanthus planifolius leaves. Compounds 1–6 were elucidated by 1- and 2-dimensional nuclear magnetic resonance experiments and high-resolution mass spectrometry analysis. The absolute configuration of compound 5 was determined through the comparison of experimental and calculated electronic circular dichroism data. Compounds 1–6 were found to exhibit antifungal activity with minimum inhibitory concentration (MIC) values of 7.8 – 250 µg/mL and racemic mixture of compound 6 exhibited weak cytotoxicity against MCF-7 and LM3 with IC50 values of 34.4 and 39.2 µM, respectively.

Methanolic extract of Woodfordia fruticosa Kurz flowers ameliorates carbon tetrachloride-induced chronic hepatic fibrosis in rats

2014 ◽  
Vol 32 (7) ◽  
pp. 1224-1236 ◽  
Author(s):  
A Nitha ◽  
SP Prabha ◽  
PN Ansil ◽  
MS Latha
Keyword(s):  
Carbon Tetrachloride ◽  
Hepatic Fibrosis ◽  
Methanolic Extract ◽  
Mass Spectrometry Analysis ◽  
Dependent Manner ◽  
Spectrometry Analysis ◽  
Collagen Iii ◽  
Male Wistar Rats ◽  
Matrix Accumulation ◽  
Woodfordia Fruticosa

Hepatic fibrosis, characterized by extracellular matrix accumulation, is the common cause of chronic liver failure and is a leading cause of morbidity and mortality worldwide. The aim of the present study was to evaluate the effect of dried flowers of Woodfordia fruticosa on carbon tetrachloride (CCl4)-induced hepatic fibrosis in rat model. Hepatic fibrosis was induced in male Wistar rats by CCl4 administration (150 μl/100 g rat weight, oral) twice a week for 10 weeks. In preventive model, administration of daily doses of methanolic extract of W. fruticosa (MEWF) at two different doses (100 mg/kg, body weight (b.w.) and 200 mg/kg, b.w.) was started 1 week before the onset of CCl4 administration and continued for 10 weeks. In curative model, MEWF at 100 and 200 mg/kg were given for last 2 weeks after the establishment of fibrosis. MEWF at a dose of 200 mg/kg was able to exert a more pronounced effect as evidenced histologically by significant reduction in fibrotic septa formation in liver tissue, immunohistochemically by abridged expression of collagen III, and also biochemically by serum and tissue antioxidant status, lipid peroxidation, and hydroxyproline level. Liquid chromatography–mass spectrometry analysis revealed the presence of confertin, quercetin methyl ether, ellagic acid, and stigmasterol in MEWF, which could be responsible for its antifibrotic activity. These results indicate the effective protection exerted by MEWF against CCl4-induced hepatic fibrosis in a dose-dependent manner.

scholarly journals GAS CHROMATOGRAPHY-MASS SPECTROMETRY ANALYSIS OF METHANOLIC LEAF EXTRACT OF CASSIA ANGUSTIFOLIA VAHL.

2016 ◽  
Vol 9 (9) ◽  
pp. 111 ◽  
Author(s):  
Shahina Parveen ◽  
Anwar Shahzad ◽  
Anamica Upadhyay ◽  
Vikas Yadav
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Vitamin E ◽  
Leaf Extract ◽  
Methanolic Extract ◽  
Mass Spectrometry Analysis ◽  
Gas Chromatography Mass Spectrometry ◽  
Spectrometry Analysis ◽  
Cassia Angustifolia ◽  
Chromatography Mass Spectrometry

ABSTRACTObjective: The leaves of Cassia angustifolia Vahl. are employed for the treatment of several human diseases. Therefore, the present study wasundertaken to determine the phytocomponents present in the methanolic extract of the leaves by gas chromatography-mass spectrometry (GC-MS)Methods: The collected leaf samples were dried and extracted in methanol. Screening of the extract was done by GC-MS which is an importanttechnique for the separation and identification of different phytochemicals.Results: The methanolic extract of the leaves of C. angustifolia revealed the presence of 45 different phytochemicals. The prevailing compounds were1 butanol, 3 methyl acetate (area % 7.47), 6, 6-dideutero-nonen-1-Ol-3 (area % 10.45), pentadecanoic acid (area % 9.22), and squalene (area %12.30). Vitamin E (area % 3.85) has also been found in the leaf extract. Some of the compounds possess biological activities.Conclusions: It can be concluded from the present study that some of the identified phytochemicals could be responsible for the medicinal value orbiological activity of the plant leaves.Keywords: Senna, Leaf extract, Methanol, Gas chromatography-mass spectrometry, Phytochemicals, Vitamin E.

scholarly journals Investigation of the In-Vivo Cytotoxicity and the In Silico-Prediction of MDM2-p53 Inhibitor Potential of Euphorbia peplus Methanolic Extract in Rats

Toxins ◽  
2019 ◽  
Vol 11 (11) ◽  
pp. 642 ◽  
Author(s):  
Yasmina M. Abd-Elhakim ◽  
Mohamed Abdo Nassan ◽  
Gamal A. Salem ◽  
Abdelkarim Sasi ◽  
Adil Aldhahrani ◽  
...  
Keyword(s):  
In Silico ◽  
Methanolic Extract ◽  
P53 Protein ◽  
Underlying Mechanism ◽  
Mass Spectrometry Analysis ◽  
Gas Chromatography Mass Spectrometry ◽  
P53 Expression ◽  
Spectrometry Analysis ◽  
Euphorbia Peplus

This study explored the probable in vivo cardiac and renal toxicities together with in silico approaches for predicting the apoptogenic potential of Euphorbia peplus methanolic extract (EPME) in rats. Cardiac and renal injury biomarkers were estimated with histopathological and immunohistochemical evaluations of both kidney and heart. The probable underlying mechanism of E. peplus compounds to potentiate p53 activity is examined using Molecular Operating Environment (MOE) docking software and validated experimentally by immunohistochemical localization of p53 protein in the kidney and heart tissues. The gas chromatography/mass spectrometry analysis of E. peplus revealed the presence of nine different compounds dominated by di-(2-ethylhexyl) phthalate (DEHP). Significant elevations of troponin, creatine phosphokinase, creatine kinase–myocardium bound, lactate dehydrogenase, aspartate transaminase, alkaline phosphatase, urea, creatinine, and uric acid were evident in the EPME treated rats. The EPME treated rats showed strong renal and cardiac p53 expression and moderate cardiac TNF-α expression. Further, our in silico results predicted the higher affinity and good inhibition of DEHP, glyceryl linolenate, and lucenin 2 to the MDM2-p53 interface compared to the standard reference 15 a compound. Conclusively, EPME long-term exposure could adversely affect the cardiac and renal tissues probably due to their inflammatory and apoptotic activity. Moreover, the in silico study hypothesizes that EPME inhibits MDM2-mediated degradation of p53 suggesting possible anticancer potentials which confirmed experimental by strong p53 expression in renal and cardiac tissues.

scholarly journals GAS CHROMATOGRAPHY AND MASS SPECTROMETRY ANALYSIS OF BIOACTIVE COMPOUNDS OF DRYOPTERIS HIRTIPES (BLUMZE) KUNTZE

2020 ◽  
pp. 119-122
Author(s):  
VALARMATHI R ◽  
NATARAJAN D
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Bioactive Compounds ◽  
Methanolic Extract ◽  
Antimicrobial Property ◽  
Mass Spectrometry Analysis ◽  
Spectrometry Analysis ◽  
Whole Plant ◽  
Low Concentrations ◽  
Anti Cancer

Objective: The objective of this study was to analyze GC–MS analysis of whole plant methanolic extract of Dryopteris hirtipes from Dryopteridaceae family. Methods: Gas chromatography and mass spectrometry analysis of whole plant extract was carried out with instrument GC–MS. Results: The methanolic extract of D. hirtipes reveals to identify more known and unknown bioactive compounds. In this study, seven major bioactive compounds were identified such as Stigmast-5-en-3-ol(56.65%), Phytol (5.39%), Lanost -8-en-3-ol-(3 β)(3.18%), Neophytadiene(2.68%), Tri-o-trimethylsilyl N-heptaflurobutryl derivative of terbutaline(2.19%), 1H-Imidazole 2-methanol(1.28%), and 8A-(2,4-Dimethyl-1-nitrilo-pent-2-yl) dioxy)tocopherone(1.0%) and low concentrations of compounds like hexadecanoic acid(0.6%). Conclusion: These identified compounds are having active pharmacological properties such as antimicrobial property, hypotension, anti-inflammatory, anti-tumor, anti-cancer, anti-hepatitis, analgesic, and antipyretic properties. However, D. hirtipes is a rare pteridophyte and used to cure many diseases, and so there need further studies to isolate and identify the specific active compounds present in it.

scholarly journals Synthesis, X-Ray Crystal Structures, and Preliminary Antiproliferative Activities of New s-Triazine-hydroxybenzylidene Hydrazone Derivatives

2019 ◽  
Vol 2019 ◽  
pp. 1-10 ◽  
Author(s):  
Assem Barakat ◽  
Fardous F. El-Senduny ◽  
Zainab Almarhoon ◽  
Hessa H. Al-Rasheed ◽  
Farid A. Badria ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cell Lines ◽  
Anticancer Agents ◽  
Hydroxyl Group ◽  
X Ray ◽  
Synthetic Strategy ◽  
Weak Activity ◽  
Hct 116 ◽  
Antiproliferative Activities ◽  
Mcf 7

We herein report a new small library of Schiff-base compounds that encompasses s-triazine and (2 or 4)-hydroxylbenzylidene derivatives. These compounds were synthesized through a hydrazone linkage connecting both the s-triazine and hydroxybenzylidene derivatives. The synthetic strategy adopted allowed the synthesis of the target compounds with excellent yields and purities as observed from their NMR (1H and 13C) and elemental analysis. Furthermore, 4f, 5b, and 5f were further confirmed by X-ray single crystal diffraction technique. The preliminary antiproliferative activities for the synthesized compounds were tested against two different cancer cell lines including breast cancer (MCF-7) and colon cancer (HCT-116). From the eighteen compounds, which have been examined, only two derivatives having piperidine moiety showed more selectivity against the two cell lines MCF-7 and HCT-116, while the others showed very weak activity. The position of the hydroxyl group in the benzylidine ring and the substituent on the s-triazine moiety has great effect on the activity of the prepared compounds. The IC50 values for the two derivatives 4a and 5a evaluated against breast cancer cells, very close to those for the chemotherapeutic drug cisplatin, are 27 µM (13.3 µg/mL), 17 µM (8.4 µg/mL), and 20 µM (6 µg/mL) for 4a, 5a, and cisplatin, respectively. These results propose the preliminary antiproliferative activity of these two derivatives may deserve further consideration for development of new derivatives as potent anticancer agents.

Synthesis, Characterization, and Biological Evaluation of Novel Naringenin Derivatives as Anticancer Agents

2020 ◽  
Vol 16 (4) ◽  
pp. 442-448 ◽  
Author(s):  
Yogesh Murti ◽  
Pradeep Mishra
Keyword(s):  
Anticancer Activity ◽  
Anticancer Agents ◽  
Colorimetric Assay ◽  
Biological Evaluation ◽  
Proton Nuclear Magnetic Resonance ◽  
Sulforhodamine B ◽  
Ic50 Values ◽  
Grinding Technique ◽  
Ht 29 ◽  
Mcf 7

Background: In the present study, a series of substituted naringenin derivatives was synthesized by Claisen–Schmidt reaction using grinding technique. Methods: Synthesized compounds were characterized on the basis of Fourier-Transform Infrared Spectroscopy (FTIR), proton Nuclear Magnetic Resonance (1H NMR), Mass Spectroscopy (MS) and elemental analysis. These derivatives were screened for anticancer activity on breast (MCF-7) and colon (HT-29) cell lines using Sulforhodamine B (SRB) colorimetric assay. Results: Results displayed improved inhibitory concentration (IC50) values of naringenin derivatives. IC50 values of 3(4-chlorobenzylidene)-5,7-dihydroxy-2(4-hydroxyphenyl)chroman-4-one are 10.35 μM (MCF-7) & 12.03 μM (HT-29), which is most potent compound in the series. These finding confirms the suitability of 3-substituted naringenin in improving the anticancer effect. Conclusion: Due to the intense interest in the development of drugs capable of inhibiting cancerous cells, naringenin derivatives may represent important precursor molecules for the therapeutic armamentarium of colon and breast cancer. Further structural modification in these structures will be of interest and may result in compounds having a better anticancer activity.

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