scholarly journals Immunohistochemical Expression of AQP2 and HSP70 in Broiler Kidney Tissue Treated with Salix tetrasperma Roxb. Extract under Heat Exposure

2021 ◽  
Vol 2021 ◽  
pp. 1-9
Author(s):  
Sugito Sugito ◽  
Etriwati Etriwati ◽  
Muslim Akmal ◽  
Erdiansyah Rahmi ◽  
Mira Delima ◽  
...  

The administration of plant extracts to broilers may be a way to mitigate the effects of heat stress. The importance of AQP2 and HSP70 compounds in maintaining the homeostasis of the chicken body when it is subjected to heat stress is well established. This study aims to determine the effect of giving the ethanolic extract of the leaves of Salix tetrasperma Roxb. on the immunohistochemical expression of AQP2 and HSP70 in exposed and unexposed broiler kidney tissue. This study used 36 samples of 28-day-old chicken kidneys. Chickens were kept in individual cages, provided with feed and drinking water ad libitum. The design used was a completely randomized design with 6 treatments and 6 replications: (a) chickens were reared in conditions exposed to heat (HS + 0); (b) chickens were reared in conditions exposed to heat and given Salix extract at a dose of 50 mg/L drinking water (HS + 50); (c) chickens were reared under heat-exposed conditions and given Salix extract at a dose of 100 mg/L drinking water (HS + 100); (d) chickens were reared in conditions without exposure to heat (n-HS + 0); (e) chickens were reared in conditions without exposure to heat and given Salix extract at a dose of 50 mg/L drinking water (nHS + 50); and (f) chickens were reared in conditions exposed without exposure to heat and given 100 mg/L drinking water (nHS + 100) of Salix extract. Salix extract was given for 24 hours and was renewed every 6 hours. The results showed that giving Salix extract 100 mg/L in drinking water to chickens exposed to heat (HS + 100) reduced the value of the H/L ratio. Giving Salix extract 50–100 mg/L in drinking water caused an upregulated AQP2 expression; on the other hand, it downregulated HSP-70 expression, in chicken kidney tubules both exposed to heat stress and nonexposed to heat stress. In conclusion, exposure to heat stress in broiler chickens and giving Salix extract can increase the formation of aquaporin 2 compounds and suppress the formation of HSP70.

2010 ◽  
Vol 10 (2) ◽  
pp. 21-26
Author(s):  
Sugito Sugito ◽  
Erdiansyah Rahmi ◽  
Azhari Azhari ◽  
M. Isa

The gain body weight and Spoilage of meat broiler which giving jaloh extract to combine with chromiumABSTRACT. A study was conducted on broiler chickens under heat stress to evaluate the effect of combined jaloh (Salix tetrasperma Roxb) extract with chromium mineral in the body weight, value of feed ratio conversion, and time to meat to become decomposed. Twenty four of 20-day old Cobb female broiler chickens were randomly assigned and divided to 4 treatment groups. Completely randomized design was used in this study. The treatments were as follows: 1) chickens given no heat stress and jaloh extract nor chromium (tCp); 2) chickens under heat stress without given jaloh extract nor chromium (Cp); 3) chickens under heat stress and given jaloh extract in 1.000 mg/lt drinking water (Cp+Ej); and 4) chickens under heat stress, given jaloh extract in 1.000 mg/lt drinking water, and chromium in 1.000 ug/lt drinking water (Cp+Ej+Cr). Heat stress given was 33 ± 1oC of cage temperature during 4 hours per day within 15 days. Jaloh extract and chromium treatments in drinking water were given at 2 hour before cage temperature reaching 33 ± 1oC and were stopped being given after 1 hour, when cage temperature back to room temperature. On the 16th day of the study chickens were measured before slaughtered. Samples obtained were chicken meat that taken from breast part (musculus pectoralis). The result suggested that either giving jaloh extract per se at the dose of 1.000 mg/lt in drinking water or combine it with chromium that given two hours before cage temperature reaching 33 ± 1oC, can prevent chickens from decreasing their body weight, decreasing value of feed ratio convertion, and extending time to meat to become decomposed.


2011 ◽  
Vol 11 (2) ◽  
pp. 8-15
Author(s):  
Sugito Sugito ◽  
Fakhrurrazi Fakhrurrazi ◽  
M. Isa

The effect of jaloh extract combined with probiotic and chromium on the haematology profiles and ND antibody vaccine of broiler given heat stressABSTRACT. The purpose of this study is to determine the respons of chicken broiler under heat stress that given drinking water contained extract jaloh combined with probiotic and Cr mineral in the profile of haematology and antibody respons of Newcastle Disease vaccine. Twenty four of 20-day old Cobb broiler chickens were randomly assigned and devided to 6 treatment groups. Completely randomized design was used in this study. The treatments were as follows: 1) chickens under heat stress, given jaloh extract, probiotic, and chromium (P1); 2. chickens under heat stress, given jaloh extract and probiotic (P2); 3. chickens under heat stress, given jaloh extract and chromium (P3); chickens under heat stress, given jaloh extract (P4); 5. chickens under heat stress without given jaloh extract nor chromium and probiotic (P5); and 6. chickens given no heat stress and jaloh extract nor chromium and probiotic (P6). Extract jaloh dose applied is 1.000 mg/lt water, dose of probiotic is 108 cfu/lt drinking water, and chromium given in the form of chloride chromium with dose 1000 ug/lt water. Local heat stress is provided by increasing cage temperature to the range of 33.0 ± 1°C during 5 hours per day u within 15 days. Jaloh extract, probiotic, and chromium treatments in drinking water were given at 2 hour before cage temperature reaching 33 ± 1oC and were stopped being given after 1 hour, when cage temperature back to room temperature. Blood was taken before chickens were ethanuatiated. The result suggested that giving jaloh extract to combine it with probiotic, and chromium has no significant effect in hematology profile. When combined extract jaloh with chromium (Cr), has potencial capability to stimulate improvement built of antibody (as immunomodulator) on broiler chicken heat stress.


2017 ◽  
Vol 5 (2) ◽  
pp. 100-105
Author(s):  
Ferdianus Nono ◽  
Dyah Lestari Yulianti ◽  
Aju Tjatur Nugroho Krisnaningsih

  ABSTRAK Tujuan penelitian ini adalah untuk mengetahui pengaruh dari penggunaan ramuan herbal terhadap income over feed cost  ayam broiler. Materi  yang digunakan pada  penelitian ini adalah: ayam broiler DOC. Penelitian ini dirancang dengan menggunakan Rancangan Acak Lengkap (RAL) yang terdiri dari 4 perlakuan dan masing-masing perlakuan terdiri dari 4 ulangan. Perlakuan yang digunakan dalam penelitian ini adalah penggunaan ramuan herbal dalam air minum dengan level penggunaan sebagai berikut : P0: tanpa penambahan ramuan herbal, P1:air + 10 % ramuan herbal, P2: air  + 15 % ramuan herbal, P3: air + 20% ramuan herbal. Variabel yang di amati  dalam penelitian ini adalah konsumsi pakan, biaya pakan, dan income over feed cost ayam broiler. Data yang diperoleh (income over feed cost) di analisis dengan menggunakan analisis ragam dengan rancangan Acak Lengkap (RAL). Hasil penelitian menunjukan bahwa biaya pakan tertinggi terdapat pada kelompok perlakuan P3 diikuti berturut-turut oleh P1, P2, P0 dan yang terendah adalah P0. Sedangkan pada P0 biaya pakan paling rendah karena tidak ada penggunaan ramuan herbal. Berdasarkan data penerimaan, penerimaan tertinggi terdapat pada kelompok kontrol P1 Rp 31.430,6  sedangkan penerimaan terandah terdapat pada kelompok P0 sebesar Rp 24.814,8.100 Rendahnya penerimaan dalam penelitian ini disebabkan oleh rendahnya bobot badan ayam broiler selama penelitian. Income Over Feed Cost selama penelitian yang tertinggi terdapat pada kelompok P3 (3.568), dan terendah terdapat pada perlakuan  P1 (979) P0 (1.964), P2 (1.937) karena biaya pakan lebih tinggi dari pada penerimaan.Ini berarti bahwa penggunaan ramuan herbal  dalam air minum berpengaruh sangat nyata (P< 0,01) terhadap Income Over Feed Cost. Berdasarkan hasil penelitian dapat disimpulkan bahwa penggunaan ramuan herbal sebagai feed additive  memberikan pengaruh sangat nyata terhadap IOFC ayam broiler.   Abstract The purpose of this study was to determine the effect of using herbal ingredients on income over feed costs of broiler chickens. The material used in this study was: broiler DOC. This study was designed using a Completely Randomized Design (CRD) consisting of 4 treatments and each treatment consisted of 4 replications. The treatment used in this study is the use of herbal ingredients in drinking water with the use level as follows: P0: without the addition of herbal ingredients, P1: water + 10% herbal ingredients, P2: water + 15% herbal ingredients, P3: water + 20% herbal concoctions. The variables observed in this study were feed consumption, feed costs, and income over feed costs for broiler chickens. The data obtained (income over feed cost) is analyzed using a variety of analysis with a completely randomized design (CRD). The results showed that the highest feed costs were found in the P3 treatment group followed by P1, P2, P0 and the lowest was P0. Whereas at P0 the cost of feed is lowest because there is no use of herbal ingredients. Based on acceptance data, the highest revenue was in the P1 control group of Rp. 31,430.6 while the lowest acceptance was in the P0 group of Rp. 24,814.8,100. The low acceptance in this study was caused by the low body weight of broiler chickens during the study. Income Over Feed Cost during the highest research was found in the P3 group (3,568), and the lowest was found in the treatment of P1 (979) P0 (1,964), P2 (1,937) because the cost of feed was higher than acceptance. drinking water has a very significant effect (P <0.01) on Income Over Feed Cost. Based on the results of the study it can be concluded that the use of herbal ingredients as additive feeds has a very significant effect on IOFC broiler chickens.


2021 ◽  
Vol 5 (1) ◽  
pp. 32-40
Author(s):  
andy andytriwibowo ◽  
◽  
Novi Eka Wati ◽  
Miki Suhadi ◽  
◽  
...  

ABSTRACT Herbal plants can be used as feed additive, on of which is the papaya plant (Carrica papaya L). The reseach was conducted on 11-27 September 2020 at street Wolter Monginsidi alley Nuri number 55, Teluk Betung North District, Bandar Lampung. This study aims to determine the effect of adding papaya leaf juice in drinking water to the performance broiler chickens. The material used was 96 broiler chickens ranging from 5-21 days of age. The method used was a Completely Randomized Design (CRD) with 4 treatments and 4 replications. The treatments given was control drinking water without additional (P0), control drinking water + 5 ml papaya leaf juice/liter of water (P1), control drinking water + 10 ml papaya leaf juice/liter of water (P2), control drinking water + 15 ml papaya leaf juice/liter of water (P3). The result showed that the addition of papaya leaf juice up to level 1,5% did not have a significant effect on the performance of broiler chickens at weeks 1, 2, and 3. Based on the reseach result it can be concluded that the addition of papaya leaf juce in drinking water up to level 1.5% has not had a significant effect (P>0,05) on performance broiler chicken. Keywords : Broiler Chicken, Papaya Leaf Juice, Drinking Water, Broiler Performance


2020 ◽  
Vol 18 (3) ◽  
pp. 74-80
Author(s):  
R Lestari ◽  
A Darmawan ◽  
Indah Wijayanti Wijayanti

This experiment was design to evaluate the effects of supplementation of Zn and Cu in inorganic or organic form on broiler’s percentage of giblets and abdominal fat of 35-day-old broiler chickens. The experiment used a completely randomized design with two treatments, 17 replicates and 32 birds per pen (816 female and 272 male). The treatments based on the type of mineral i.e. inorganic (T1) and organic (T2). The types of mineral did not affected the weight and percentage of weight of bursa fabricius, lymph, heart, bile gland, kidney, and abdominal fat. The types of mineral did not affected the weight  of thymus, liver, proventriculus, pancreas, and gizzard, but were affected their percentage of weight (p<0.05). The types of mineral were affected  the length and percentage of weight of duodenum, jejunum,and ileum (p<0.05) . The types of mineral were affected the length of secca, but did not affected its percentage of weight (p<0.05). The types of mineral did not affected the length of colon, but were affected its  percentage of weight (p<0.05). The supplementation of mineral Cu and Zn in organic form has better absorption of nutrient and positive effect to health of chickens in stress due to heat stress. Key words:        broiler, copper,  inorganik mineral, organik mineral,  zinc


Author(s):  
Rizki Tikadewi Noviani ◽  
Purnama Edy Santosa ◽  
Sri Suharyati ◽  
Siswanto Siswanto

The purpose of this study was to determine the description of the blood profile especially hemoglobin and hematocrit of male broiler chickens that given Nigella sativa (black cumin seed). This research was conducted on 18 December 2019-16 January 2020 at the Integrated Field Laboratory, Faculty of Agriculture, Lampung University and Pramitra Biolab Indonesia Clinical Laboratory, Bandarlampung. This study used a completely randomized design (CRD) with 4 treatments and 3 replications. The treatments given in this study were broiler chickens drinking water without Nigella sativa (P0), drinking water with 36 mg/kg BW/day of Nigella sativa (P1), drinking water with 72 mg/kg BW/day of Nigella sativa (P2), drinking water with 144 mg /kg BW/day of Nigella sativa (P3). Data were analyzed descriptively (histogram). The results showed that application of Nigella sativa at a dose of 144mg /kg BW/day (P3) was the optimal dose to increase hemoglobin levels and hematocrit values of male broiler chicken. Keywords: Black cumin, hemoglobin, hematocrit, immunomodulator, male broiler, Nigella sativa


Author(s):  
Ibrahim Hadist

Abstrak Penelitian bertujuan untuk mengetahui sampai sejauh mana pengaruh pemberian herbal yang mengandung probiotik dalam air minum terhadap konsumsi ransum segar,konsumsi bahan kering, konsumsi energi ransum, konsumsi protein ransum, dan konsumsi serat kasar ransum pada ternak ayam broiler. Penelitian ini menggunakan 100 ekor DOC broiler yang ditempatkan secara acak kedalam 20 kandang, dan setiap kandang terdiri dari 5 ekor ayam. Penelitian menggunakan metode experimental dengan Rancangan Acak Lengkap (RAL) dengan empat perlakuan air minum dan lima ulangan. Perlakuan air minum yang digunakan yaitu air minum yang tidak mengandung probiotik 0% (R1); mengandung vitamin dan antibiotik (R2); mengandung 1 ml herbal yang mengandung probiotik (R3); dan mengandung 2 ml herbal yang mengandung probiotik (R4). Hasil penelitian menunjukan bahwa penambahan herbal yang mengandung probiotik tidak berpengatuh terhadap palatabilitas ransum ayam broiler. Kata kunci : herbal, probiotik, air minum, palatabilitas, ayam broiler Abstract The object of study was evaluate the extent of the effect of herbs that contain probiotics in drinking water on feed consumption, dry matter intake, feed energy consumption, the consumption of dietary protein, and crude fiberconsumption in broiler chickens. This reseach used 100 broiler at one days of age, were randomly placed into 20 cages, each cage consisted of five chickens. The methode of research was experimental designed by completely randomized design (CRD) with four treatments and five replications of drinking water. The treatments consisted of drinking water that does not contain probiotics 0% (R1); drinking water were contain vitamins and antibiotics (R2); drinking water were contain 1 ml herbs with probiotics (R3); drinking water were contain 2 ml herbs with probiotics (R4). The results showed that the addition of herbs that contain probiotics do not affect the feed palatability of broiler chicken. Keywords : Herb, probiotic, drinking water, palatability, broiler chicken


2020 ◽  
Vol 4 (1) ◽  
pp. 42-50
Author(s):  
Jet Saartje Mandey ◽  
Cherly Joula Pontoh

The study was conducted to analyze the phytochemicals of basil leaves (Ocimum x citriodorum L.)and to evaluate the effect of basil leaves juice as an additive on nutrient digestibility of broilerchicken diet. This study used 20 male broilers (35 days old). The research parameters werephytochemical, proximate, and digestibility analysis. The data of basil leaves phytochemical andproximate analysis were analyzed using descriptive analysis. The digestibility were analyzed usinga Completely Randomized Design (CRD) by 4 treatments and 5 replications. The research wasconducted for 10 days consisting of 7 days for maintenance period and 3 days for total collectionperiod. The digestibility treatments consisted of 0 mL, 10 mL, 20 mL, and 30 mL basil leaves juice/Ldrinking water. The results showed that giving basil leaves juice had a good effect on nutrientdigestibility of broiler chickens and could be used up to 20 mL/L in drinking water.


2020 ◽  
Vol 46 (5) ◽  
Author(s):  
S. O. Omoikhoje ◽  
M. O. Okooza ◽  
R. O. Okeje ◽  
F. E. Peter-Paul

Carcass traits and internal organs of one hundred and twenty, one day-old broiler chicksmaintained on different dosages of coffee weed leaf ethanolic extract for eight weeks werestudied. Five dosages (0, 25, 50, 75 and 100mL) of coffee weed leaf ethanolic extract(CWLEE) per litre of drinking water (T1 T2 , T3 , T4 and T5 ) were used. The birds were allocated \to the various treatment groups in a completely randomized design (CRD) with each treatment replicated three times (8 birds per replicate). Data on carcass traits revealed that live weight (2.50kg/bird), defeathered (2.30kg/bird) and eviscerated (2.00kg/bird) weights as well as dressing percentage (81.69%) were significantly (P<0.5) higher among birds on 50mL of CWLEE compared to those on other treatment groups. Similarly, the relative weights of drumstick (16.60%) and thigh (22.28%) were also significantly (P<0.05) better in broiler chickens administered 50mL CWLEE. Relative organ weights of broiler showed that weight of heart, lungs, gizzard, bursa, spleen and kidney were significantly (P < 0.05) influenced by the treatments. It is therefore inferred that birds administered 50mL CWLEE gave better carcass quality and functioning of the vital organs and therefore can be recommended for broiler chickens.


2014 ◽  
Vol 14 (1) ◽  
pp. 71-75 ◽  
Author(s):  
Meisji Liana Sari ◽  
Fitri Nove Liya Lubis ◽  
Lia Dwi Jaya

(The Effect of Wood Vinegar Through to The Drinking Water on The Quality of Broiler Chicken Carcass)ABSTRACT. This aims of this research was to determine the percentage of carcass, commercial carcass parting and meat bone ratio (breast, thigh and wing) of broiler consumed wood vinegar. This research was assigned into used Completely Randomized Design (CRD) with 5 different treatments and each 3 replicates. The treatments were dose of wood vinegar, R0 = water + 0% wood vinegar, R1 = water + 0.25% wood vinegar, R2 = water + 0.5% wood vinegar, R3 = water + 0.75% wood vinegar, R0 = water + 1% wood vinegar. The observed parameters were percentage of carcass, commercial sliced carcass parting and meat bone ratio. The results showed that wood vinegar in the drinking water did gave not significantly (P0,05) effect to the percentage of carcass, commercial sliced carcass parting and meat bone ratio of broiler chickens. It is concluded that Liquid wood vinegar can be used for broiler chickens until 1%.


Sign in / Sign up

Export Citation Format

Share Document