scholarly journals Nanoelicitors Application Promote Antioxidant Capacity of Asparagus officinalis (In Vitro)

2021 ◽  
Vol 11 (3) ◽  
pp. 259-265
Author(s):  
Reza Mohammadhassan ◽  
◽  
Annahid Ferdosi ◽  
Shiva Malmir ◽  
Alexander Marcus Seifalian ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Side Effects ◽  
Medicinal Plant ◽  
Antioxidant Capacity ◽  
Natural Antioxidants ◽  
Selenium Nanoparticles ◽  
Asparagus Officinalis ◽  
Dpph Assay ◽  
Male And Female ◽  
Modern Agriculture

Nanoparticles recently play remarkable roles in modern agriculture and biotechnology due to specific exclusively physicochemical and biological characteristics of the particles. In recent years, nanoparticles are been using as fertilizers and elicitors to improve crops. Nutritionists are constantly seeking natural antioxidants without side effects to using for healthcare and treatment. Asparagus officinalis L. as medicinal plant treated by iron (0, 10, 50 and 100 mg/L) and selenium (0, 0.5,and 1 mg/L) nanoparticles as nano elicitors. Then the antioxidant capacity of A. officinalis L. was detected and measured by α, α-diphenyl-β-picrylhydrazyl (DPPH) assay, for assessment of the antioxidant activity. The iron nanoparticlesconcentration significantly increases the antioxidant activity of both male and female asparagus stem, as well as selenium nanoparticles. When combined iron and selenium used as nano elicitors then cause the antioxidant activity significantly decreases. But the integration of two nano elicitors (iron and selenium) decreased antioxidant capacity while the use of nano-selenium could enhance antioxidant capacity. The application of nano elicitor increased antioxidant capacity in the female stem than male.Nanoparticles recently play remarkable roles in modern agriculture and biotechnology due to specific exclusively physicochemical and biological characteristics of the particles. In recent years, nanoparticles are been using as fertilizers and elicitors to improve crops. Nutritionists are constantly seeking natural antioxidants without side effects to using for healthcare and treatment. Asparagus officinalis L. as medicinal plant treated by iron (0, 10, 50 and 100 mg/L) and selenium (0, 0.5,and 1 mg/L) nanoparticles as nano elicitors. Then the antioxidant capacity of A. officinalis L. was detected and measured by α, α-diphenyl-β-picrylhydrazyl (DPPH) assay, for assessment of the antioxidant activity. The iron nanoparticlesconcentration significantly increases the antioxidant activity of both male and female asparagus stem, as well as selenium nanoparticles. When combined iron and selenium used as nano elicitors then cause the antioxidant activity significantly decreases. But the integration of two nano elicitors (iron and selenium) decreased antioxidant capacity while the use of nano-selenium could enhance antioxidant capacity. The application of nano elicitor increased antioxidant capacity in the female stem than male.

scholarly journals Evaluation of Antioxidant and Cytotoxic Effect of Selenium Nanoparticles Synthesised Using Capparis decidua

2020 ◽  
pp. 60-66
Author(s):  
P. S. Thana Lakshme ◽  
Preetha S ◽  
M. Jeevitha ◽  
S. Rajeshkumar
Keyword(s):  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Antioxidant Capacity ◽  
Brine Shrimp ◽  
Cytotoxic Effect ◽  
Cost Effective ◽  
Selenium Nanoparticles ◽  
Inert Material ◽  
Dpph Assay ◽  
Capparis Decidua

Background: Among the nanoparticles, selenium nanoparticles (SeNP) are one of the most extensively studied as Selenium has zero oxidation state, non toxic and biologically inert material. This is the reason why Selenium is considered as a major nanoparticulate. In this study SeNPs were extracted from the fruit of Capparis decidua which is a xerophytic small herb. Aim: The aim of the present study is to evaluate the cytotoxic effect and antioxidant capacity of selenium nanoparticles. Materials and Methods: In this study SeNPs were extracted from the fruit of Capparis decidua which is a xerophytic small herb. The cytotoxic effect of SeNPs was evaluated using Brine Shrimp assay and the antioxidant activity was determined using DPPH assay considering ascorbic acid as the standard. Results: From the study of this assay the shrimps introduced  into the well were almost alive in different concentrations and this indicates that there is no cytotoxicity in selenium nanoparticles. The percentage inhibition of Selenium nanoparticles in 10 µl was 15.4 0.1, 20 µl was 38.36 0.15, 30 µl was 45.3 0.1, 40 µl was 59.6 0.15 and 50 µl was 65.6  0.1. It can be inferred that percentage inhibition increases with increase in concentration but it was less when compared to the percentage inhibition of the standard. Conclusion: The selenium nanoparticles extracted from Capparis decidua do not have any cytotoxic effect on shrimps. The SeNPs possessed significant antioxidant activity in increasing concentrations compared to the standard used. Thus SeNPs are biologically useful and can be used as eco-friendly, cost effective and efficient biomedical agents and therapeutics.

scholarly journals Comparative Characteristics of Determination of Antioxidant Activity of Sea Buckthorn Fruits by Various Methods

2019 ◽  
Vol 8 (4) ◽  
pp. 48-52
Author(s):  
O. V. Trineeva
Keyword(s):  
Antioxidant Activity ◽  
Medicinal Plant ◽  
Plant Material ◽  
Natural Antioxidants ◽  
Sea Buckthorn ◽  
In Vitro Tests ◽  
Medicinal Plant Material

Introduction. Recently, much attention has been paid to the primary assessment of the pharmacological effect of various drugs using in vivo and in vitro tests. It is known that such a medicinal plant as sea buckthorn, in its phytochemical composition is rich in natural antioxidants: carotenoids, tocopherols, flavonoids, ascorbic acid, etc. In some publications there is information about the antioxidant activity of sea buckthorn and fatty oil based on them. However, information on the comparative characteristics of the use of various methods for determining the antioxidant activity of this type of medicinal plant material and the results obtained are not found in the scientific literature.Aim. The aim of this work was a comparative determination of the antioxidant activity of medicinal plant material of buckthorn fruits of various species of buckthorn.Materials and methods. The total antioxidant activity of water and water-alcohol extracts from the fruits of sea buckthorn fruits was determined using various techniques recommended in the literature. The antioxidant activity of the extracts was determined by permanganometric titration, in vitro inhibition of adrenaline autooxidation, and also in a biological model, Parametium caudatum cell culture.Results and discussion. The effect of the extractant polarity on the value of antioxidant activity was studied. It was found that the highest content of antioxidants in the extraction is observed when using 96 % ethanol as an extractant.Conclusion. Using three methods, the prospects of using sea buckthorn fruits and preparations based on them as a source of antioxidants are shown. 

scholarly journals Physicochemical properties and antioxidant capacity of propolis of stingless bees (Meliponinae) and Apisfrom two regions of Tocantins, Brazil

2016 ◽  
Vol 46 (1) ◽  
pp. 61-68 ◽  
Author(s):  
Karuane Saturnino da Silva ARAÚJO ◽  
Joaquim Francisco dos SANTOS JÚNIOR ◽  
Marcello Otake SATO ◽  
Fernanda Dias Bartolomeu Abadio FINCO ◽  
Ilsamar Mendes SOARES ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Antioxidant Capacity ◽  
High Performance ◽  
Stingless Bees ◽  
Total Concentration ◽  
Natural Antioxidants ◽  
Physicochemical Characteristics ◽  
Phenol Compounds

ABSTRACTThe composition of propolis depends on time, vegetation and the location of the collection area. The objective of this study was to determine the physicochemical characteristics, the concentration of phenol compounds and the antioxidant capacity of propolis of native stingless bees (Meliponinae)and Apis from the State of Tocantins. Extraction with 80% ethanol (v/v) was performed in order to obtain the extracts. Parameters examined were: propolis mass loss by desiccation at 105 ºC, ashes, wax concentration and pH. Furthermore, the propolis antioxidant activity was measured, as well as the total concentration of phenol compounds. The extracts were also analyzed by high performance liquid chromatography. The total concentration of phenol compounds varied between 121.78 and 631.29 (mg GAE g-1). The antioxidant activity expressed by the value of CE50 varied between 29.81 and 845.38 µg mL-1. High performance liquid chromatography analysis allowed us to infer the existence of phenol compounds. The results indicated that the studied propolis samples constitute good sources of natural antioxidants. The variety of phenol compounds identified in this study, and the diverse biological functions reported in literature for these compounds indicated that this stingless bee propolis (Meliponinae) and Apis has a high pharmacological potential.

Antioxidant activity, total phenolic and flavonoids contents of three herbs used as condiments and additives in pickles products

2013 ◽  
Vol 59 (3) ◽  
pp. 51-62 ◽  
Author(s):  
Abdollah Ghasemi Pirbalouti ◽  
Milad Setayesh ◽  
Amir Siahpoosh ◽  
Hamid Mashayekhi
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Capacity ◽  
Total Phenolic Content ◽  
Food Industry ◽  
Phenolic Content ◽  
Food Additives ◽  
Total Phenolic ◽  
Endemic Plants ◽  
Dpph Assay ◽  
Frap Assay

Abstract Heracleum lasiopetalum Boiss, Kelussia odoratissima Mozaff., and Echinophora platyloba DC. belong the Apiaceae family. They are Iranian endemic plants. These three herbs have been used as food additives in traditional preparations such as pickles. Antioxidant activity (AA) of methanol extracts (ME) of the plants was evaluated by three assays, including DPPH, FRAP, and TEAC. From all three assays, comparing all the MEs for their IC50 and EC1 values, E. platyloba had the highest AA. Total phenolic content (TPC) of the extracts ranged from 74 to 120 mg TAE/g. The extract of H. lasiopetalum exhibited the highest TPC. The flavonoids content (FC) of the extracts ranged from 7.63 to 14.52 mg RE/g, from which the extract of E. platyloba had the highest flavonoids concentration. A positive correlation between the FC and AA in DPPH assay was found. A significant correlation was also found between the TPC and AA in FRAP assay. These results suggested that the level of AA in these plants varied in a great extent. Our results indicated that extract of E. platyloba could be an important dietary source of flavonoids compounds with high antioxidant capacity. In addition, E. platyloba can be used as an alternative preservative and natural flavor instead of synthetic ones in food industry (especially pickles)

scholarly journals Effect of Brewing Water on the Antioxidant Capacity of Green Tea Infusion with DPPH Assay

2022 ◽  
Vol 2022 ◽  
pp. 1-8
Author(s):  
Qing-Qing Cao ◽  
Yan-Qing Fu ◽  
Cheng-Bin Zhang ◽  
Yan Zhu ◽  
Jun-Feng Yin ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Synergistic Effect ◽  
Metal Ions ◽  
Antioxidant Capacity ◽  
Green Tea ◽  
Ph Value ◽  
Major Contributor ◽  
Dpph Assay ◽  
Tea Infusion ◽  
Selection Of

Brewing water plays a crucial role in flavor and potential healthy functions of tea infusion. In this study, seven water samples with different physicochemical properties were selected to brew green tea. Results showed that the brewing water with higher minerals level and pH value would reduce the yield of catechins in tea infusion, which in turn caused the decrease of antioxidant activity to a large extent. Besides, it was found that EGCG, as a major contributor to the antioxidant activity of green tea infusion, was influenced differently by different metal ions, among which Ca2+/Mg2+ could enhance the antioxidant activity of EGCG solutions with different concentration through synergistic effect, particularly Ca2+, and the effect was more markable at a higher EGCG concentration. These results offered theoretical direction to the selection of tea brewing water for consumers and gave a new sight to the effects of metal ions on the antioxidant capacity of EGCG.

The Antioxidant Activity and Phytochemical Analysis of Medicinal Plant Veronica Biloba

2020 ◽  
Vol 5 (1) ◽  
Author(s):  
AMIR HASSAN ◽  
Himayat Ullah ◽  
Muhammad Israr
Keyword(s):  
Antioxidant Activity ◽  
Medicinal Plant ◽  
Ethyl Acetate ◽  
Biological Activities ◽  
Continuous Process ◽  
Natural Antioxidants ◽  
Positive Control ◽  
Phytochemical Analysis ◽  
Uniform Size ◽  
Health Related

A medicinal plant veronica genus has 450 well known species and found across both temperate and hemisphere region their 26 species are endemic and known in a total of 79 popular species and are widely utilized throughout the world due to important biological activities. In this study fully powdered uniform size specie veronica biloba plant taken in porous bag were manually subjected to soxhlet hot continuous process for cyclization of extraction using ethanol (300 mL) a concentrated dried extract obtained after solvent evaporation. Furthermore, liquid-liquid extracted fractions as water, dichloromethane, n-hexane, and ethyl acetate yields results founds polar fraction with highest percentage (water 47.51 %). The phytochemical screening of veronica biloba has shown all major compounds entirely present in extracts. One of the primitive phenolic compound flavonoid is present in plant and show potency towards antioxidants. All the extracted fractions of plant showed excellent antioxidant activity using a stable DPPH (1,1-diphenyl-2-picryl-hydrazyl) method at concentration (range from 31.25 to 500µg/mL). Primary our ethyl acetate extract fraction showed highest inhibition potential at IC50 = 1.70±0.05µg/mL which is much closer to a standard positive control Propyl gallate showed IC50 = 1.6±0.05µg/mL percent potential. The purification and isolation of these extract is important which can provide us help in novel antioxidants discovery also natural antioxidants currently in cosmetics products, food and therapeutics health related products significantly demanded because they are very effective, efficient and harmless as compared to synthetic one.

Antioxidant Activity In Vitro of Selenium Polysaccharide of Thlaspi caerulescens L. Rich in Selenium

2012 ◽  
Vol 518-523 ◽  
pp. 5518-5522 ◽  
Author(s):  
Chi Zhang ◽  
Xing Ping Liu
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Capacity ◽  
Natural Antioxidants ◽  
Mining Area ◽  
Thlaspi Caerulescens ◽  
Dose Effect ◽  
Atomic Fluorescence Spectrometry ◽  
Selenium Content ◽  
Total Selenium

The antioxidant capacity of the human body is closely related to its ability of disease resistance and anti-aging. It is a current research focus to find effective anti-oxidants used in medicine, food, health care, beauty make-up from natural plants. In this paper, we studied the content of selenium in selenium polysaccharide and its antioxidant activity in vitro of Thlaspi caerulescens L. produced from Yutangba of Enshi selenium mining area. The double tracts atomic fluorescence spectrometry is used to detect the total selenium content in the sample and that of selenium polysaccharide in the active ingredient of the sample. The salicylic acid method and pyrogallol autoxidation method are used to detect the scavenging activity of selenium polysaccharide on hydroxyl radicals and superoxide anion. Experiments show that the total amount of selenium in Thlaspi caerulescens L. is 708ug.g-1, while the amount of selenium in selenium polysaccharide is 98.1ug.g-1, accounting 13.86% of the total selenium content. Meanwhile, selenium polysaccharide in samples is with significant antioxidant capacity, and it shows a certain dose-effect relationship. Selenium polysaccharide has stronger anti-oxidation ability than pure polysaccharide compared with non-selenium polysaccharide. Therefore, plants rich in selenium are better natural antioxidants. This study will provide the necessary experimental basis for the effective and rational development and utilization of Thlaspi caerulescens L. rich in selenium

scholarly journals Antioxidant Capacity and Phenolic Content of four Myrtaceae Plants of the South of Brazil

2011 ◽  
Vol 6 (7) ◽  
pp. 1934578X1100600 ◽  
Author(s):  
Marcos José Salvador ◽  
Caroline C. de Lourenço ◽  
Nathalia Luiza Andreazza ◽  
Aislan C.R.F. Pascoal ◽  
Maria Élida Alves Stefanello
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Capacity ◽  
Transfer Reaction ◽  
Phenolic Content ◽  
Hydrogen Atom Transfer ◽  
Preliminary Evaluation ◽  
Dpph Assay ◽  
The South ◽  
Ethanolic Extracts ◽  
South Of Brazil

Antioxidant compounds can be useful to prevent several degenerative diseases or as preservative in food and toiletries. Species of the Myrtaceae family are able to accumulate phenolic substances and those are closely related to the antioxidant activity due to their capacity to scavenge free radicals, protect against lipid peroxidation and quench reactive oxygen species. These facts prompted us to investigate the antioxidant capacity of the ethanolic extracts of the leaves of four Myrtaceae plants collected of the south of Brazil: Eugenia chlorophylla O. Berg., Eugenia pyriformis Cambess, Myrcia laruotteana Cambess and Myrcia obtecta (Berg) Kiacrsk. The antioxidant potential was performed using the DPPH (a single electron transfer reaction based assay) and ORAC (Oxygen Radical Absorbance Capacity, a hydrogen atom transfer reaction based assay) assays. Moreover, the total soluble phenolic content was also measured using the Folin-Ciocalteu reagent. A preliminary evaluation of the ethanolic extracts of these Myrtaceae plants revealed high levels of phenolic compounds (343.7-429.3 mg GAE) as well as high antioxidant activity according to both methods (1338 a 3785 μmol of TE/g of extract in ORAC and SC50 in the range of 1.70 and 33.7 μg/mL in the DPPH). The highest antioxidant activity obtained by DPPH assay was exhibited by ethanol extract of the leaves of E. pyriformis (1.70 μg/mL), followed by extracts of M. laruotteana (3.38 μg/mL) and M. obtecta (6.66 μg/mL). In comparison with controls, in the DPPH assay, the extract of E. pyriformis was more active than trolox (SC50 = 2.55 μg/mL), while the extracts of M. laruotteana and M. obtecta were more actives than quercetin (SC50 = 7.80 μg/mL). In the ORAC assay, all species also show good antioxidant capacity (>1000 μmol of TE/g). Initial HPLC-UV/DAD and ESIMS confirmed the presence of phenolic acids constituents in the ethanol extracts. The results indicate the presence of compounds possessing promising antioxidant/free-radical scavenging activity in the analyzed extracts of Myrcia and Eugenia plants of the south of Brazil.

scholarly journals Effect of flavonoids content on antioxidant activity of commercial cosmetic plant extracts

2013 ◽  
Vol 59 (3) ◽  
pp. 63-75 ◽  
Author(s):  
Paulina Malinowska
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Capacity ◽  
Plant Extracts ◽  
Natural Antioxidants ◽  
Trolox Equivalent Antioxidant Capacity ◽  
Antioxidant Power ◽  
Trolox Equivalent ◽  
Ferric Reducing Antioxidant Power ◽  
The Relationship ◽  
Herb Extracts

Abstract The aim of this work was to evaluate the antioxidant capacity and flavonoids content in 10 commercial cosmetic plant extracts used in cosmetics industry. Antioxidant activity of plant extracts were measured using two methods: FRAP (Ferric Reducing Antioxidant Power) and TEAC (Trolox Equivalent Antioxidant Capacity). The relationship between flavonoids content and antioxidant capacity of plant extracts were checked. As a result of this research it was found that FRAP and TEAC values of plant extracts significantly depend on the flavonoids content. The highest antioxidant activity, both in FRAP and TEAC assays, was observed for arnica flowers, hawthorn flowers and lungwort herb extracts. These extracts can be used as source of natural antioxidants for the prolongation of the oxidative stability of cosmetic products. Additionally, they can replace synthetic antioxidants

scholarly journals Impact of Fermentation on Phenolic Compounds and Antioxidant Capacity of Quinoa

Fermentation ◽  
2021 ◽  
Vol 7 (1) ◽  
pp. 20
Author(s):  
Francesca Melini ◽  
Valentina Melini
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Antioxidant Capacity ◽  
Phenolic Compound ◽  
Chenopodium Quinoa ◽  
Natural Antioxidants ◽  
Gluten Free ◽  
Good Source ◽  
Nutritional Profile ◽  
Seed Fermentation

Quinoa (Chenopodium quinoa Willd.) is increasingly singled out as a healthy food with an excellent nutritional profile. Besides being suitable for gluten-free diets, it is rich in proteins of excellent quality and is a good source of minerals and vitamins, as well as of natural antioxidants, such as phenolic compounds. The aim of this work is to present how fermentation can affect phenolic compound content and antioxidant capacity of quinoa. It emerged that fermentation can be used to increase phenolic compound content and antioxidant capacity in both quinoa seeds and flours. The use of fermented quinoa flours allowed obtaining bread and pasta richer in phenolic compounds and with a greater antioxidant capacity. Fungi are the main starters used in quinoa seed fermentation, while Lactobacillus strains have been applied to produce sourdoughs. Quinoa has been also fermented to obtain yogurt-like beverages with a higher content in phenolic compounds and a greater antioxidant activity. Strains of Lactobacillus sp. and Bifidobacterium sp. have been used as starters.

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