Abstract P45: White Blood Cell Counts in Aneurysmal Subarachnoid Hemorrhage Patients as a Predictor of Cardiac Injury

Introduction: Up to 50% of subarachnoid hemorrhage (SAH) patients develop cardiac injury. The relationship between early systemic inflammation and cardiac injury after SAH is unknown. Here we examined changes in blood leukocyte counts and their relationship to cardiac dysfunction. Methods: We reviewed the medical records of 288 SAH patients admitted to our Comprehensive Stroke Center. Patients were dichotomized based on elevated (≥0.04ng/mL) or normal (<0.04ng/mL) troponin I (TnI). Demographics and labs from admission were then compared among the two groups by Chi-Square or Mann-Whitney test. Ejection fraction (EF) was stratified into low (<50%), normal (50-70%), or high (>70%) from echocardiogram data. We performed univariate and multivariate logistic regression to establish the relationship between blood leukocyte counts and cardiac injury. Results: Of 288 SAH patients, 241 had TnI levels performed at the time of admission and 119 (49.4%) of these had elevated TnI on admission. Patients with elevated TnI were significantly older, had higher grade SAH, abnormal EF, and were more likely to have hypertension and dyslipidemia. 10 (4.1%) had low EF while 58 (24.1%) had high EF on admission echocardiogram. In univariate analysis, total leukocyte count (p<0.0001), absolute neutrophil count (p=0.037), absolute monocyte count (p=0.014), and neutrophil-lymphocyte ratio (p=0.010) were associated with elevated TnI. Multivariate analysis adjusting for covariates showed that only total leukocyte count remained a significant predictor of elevated TnI (OR = 1.104, 95% CI= 1.020 - 1.195; p=0.014). Receiver operating characteristic (ROC) analysis demonstrated that adjusted total leukocyte count distinguishes between SAH patients with normal and elevated TnI (area under the curve = 0.787, p=0.001), with the optimal cutoff being 0.521 (sensitivity of 67.0% and specificity of 80.6%). Conclusions: Blood total leukocyte count is an independent predictor of cardiac injury in SAH patients. This highlights the role of inflammation in mediating cardiac dysfunction after brain hemorrhage, and raises questions regarding the potential of anti-inflammatory therapy for cardioprotection in SAH.

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Immunostimulatory properties of bigroot geranium (Geranium macrorrhizum L.) extract

Medicina ◽

10.3390/medicina43010008 ◽

2007 ◽

Vol 43 (1) ◽

pp. 60 ◽

Cited By ~ 1

Author(s):

Vilma Jurkštienė ◽

Anatolijus Kondrotas ◽

Egidijus Kėvelaitis

Keyword(s):

T Lymphocytes ◽

B Lymphocytes ◽

Peripheral Blood ◽

Leukocyte Count ◽

Ethanol Extract ◽

Food Supplement ◽

Total Leukocyte Count ◽

Control Group ◽

Case Group ◽

Cell Counts

The aim of the study was to investigate the immunostimulatory properties of bigroot geranium. Material and methods. Possible nonspecific characteristics of bigroot geranium were evaluated by the total leukocyte count in the peripheral blood, and qualitative changes of blood were assessed using Shilling’s formula by evaluating changes in lymphocyte counts. In addition, we also studied changes in the counts of Tcell precursors in the thymus and B lymphocytes in the spleen. Ethanol extract of the leaves of bigroot geranium was produced at the Department of Food Technology, Kaunas University of Technology. Studies were performed on mice Bl 57 (n=21). The control group (n=7) received distilled water at a dose of 1 mL/day. The second and third groups received 1% and 10% extract of bigroot geranium, respectively, as a food supplement. Changes in cell counts were investigated after 4 weeks following the initiation of the trial. Results. After a 4-week administration of 1% extract of bigroot geranium (1 mL/day) (mice group, n=7), leukocyte count in the peripheral blood increased to 6.1×109 cells/L, and lymphocyte count – to 70%, but changes were not statistically significant. The other case group of mice (n=7) received 10% extract of bigroot geranium for 4 weeks at a dose of 1 mL/day. In this group, leukocyte count in the peripheral blood increased statistically significantly from 4.4×109 cells/L to 7.2×109 cells/L (p<0.01), and lymphocyte percentage – from 52% to 80% (p<0.001), as compared to control. Thymocyte (T lymphocytes) counts in thymus and splenocyte (B lymphocytes) counts in the spleen showed a tendency to increase after the administration of 1% and 10% extracts. After a 4-week administration of 1% extract of bigroot geranium, thymocyte and splenocyte counts increased from 0.342×106 cells to 0.372×106 cells per mg of tissue and from 0.395×106 cells to 0.405×106 cells per mg of tissue, respectively, as compared to control group (p>0.1). After the administration of 10% extract of bigroot geranium, thymocyte count increased to 0.488×106 cells per mg of tissue (p<0.01), and splenocyte count – to 0.504×106 cells per mg of tissue (p<0.01). Conclusion. The extracts of the leaves of bigroot geranium increased leukocyte count and lymphocyte percentage in the peripheral blood, and after a 4-week administration of 10% extract of bigroot geranium, a statistically significant increase in the counts of T lymphocytes (in the thymus) and B lymphocytes (in the spleen) was observed. The immunostimulatory effect depends on the dose of the extract.

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Prediction of leukocyte counts during paediatric acute lymphoblastic leukaemia maintenance therapy

Scientific Reports ◽

10.1038/s41598-019-54492-5 ◽

2019 ◽

Vol 9 (1) ◽

Cited By ~ 1

Author(s):

Santeri Karppinen ◽

Olli Lohi ◽

Matti Vihola

Keyword(s):

Maintenance Therapy ◽

Acute Lymphoblastic Leukaemia ◽

Lymphoblastic Leukaemia ◽

Leukocyte Count ◽

Peripheral Blood Leukocyte ◽

Reactive Protein ◽

Leukocyte Counts ◽

Nonlinear State Space Models ◽

Blood Leukocyte ◽

The Individual

AbstractMaintenance chemotherapy with oral 6-mercaptopurine and methotrexate remains a cornerstone of modern therapy for acute lymphoblastic leukaemia. The dosage and intensity of therapy are based on surrogate markers such as peripheral blood leukocyte and neutrophil counts. Dosage based leukocyte count predictions could provide support for dosage decisions clinicians face trying to find and maintain an appropriate dosage for the individual patient. We present two Bayesian nonlinear state space models for predicting patient leukocyte counts during the maintenance therapy. The models simplify some aspects of previously proposed models but allow for some extra flexibility. Our second model is an extension which accounts for extra variation in the leukocyte count due to a treatment adversity, infections, using C-reactive protein as a surrogate. The predictive performances of our models are compared against a model from the literature using time series cross-validation with patient data. In our experiments, our simplified models appear more robust and deliver competitive results with the model from the literature.

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Comparative hematological analysis of Morada Nova and Santa Inês ewes in all reproductive stages

Pesquisa Veterinária Brasileira ◽

10.1590/s0100-736x2017000400017 ◽

2017 ◽

Vol 37 (4) ◽

pp. 408-414

Author(s):

Leílson R. Bezerra ◽

Wagner D.C. Oliveira ◽

Tairon P.D. Silva ◽

Jacira N.C. Torreão ◽

Carlo A.T. Marques ◽

...

Keyword(s):

Blood Cell ◽

Leukocyte Count ◽

Animal Health ◽

Late Pregnancy ◽

Total Leukocyte Count ◽

Productive Capacity ◽

Important Indicator ◽

Cell Counts ◽

Hematological Analysis ◽

Santa Inês

ABSTRACT: Hematologic analysis provides reliable information on the health status of animals. It is an important variable in the assessment of adaptive and productive capacity of breeds under unfavorable environmental conditions. It is an assessment that combined with other genetic and environmental factors and management can become useful for the future sustainability of mainstream agriculture in a hot environment. Adaptive capacity is affected by some factors such as breed, pregnancy and lactation. Therefore, hematological profile is an important indicator of animal health and production. The objective of this study was to perform the comparative hematological analysis of Santa Inês and Morada Nova breeds (native ewes from Brazil) in all different reproductive stages. Twenty Santa Ines and 20 Morada Nova sheep distributed in a completely randomized design in a split-plot arrangement over time were used. To obtain blood counts, blood samples were collected by jugular venipuncture every 14 days, always in the morning, before the animals were released into the pasture. The Morada Nova breed had higher hemoglobin and total leukocyte count than Santa Inês breed. Regarding the influence of pregnancy and puerperium on the eritrogram, there was an increment in red blood cell, hemoglobin and packed cell volume of both breeds in middle pregnancy. However, had a reduction in late pregnancy. There were a reestablishment of the blood cell counts during the puerperium period. It was verified an increase on neutrophil: lymphocyte ratio indicated that ewes in the late pregnancy, postpartum and puerperium were under stress conditions compared to others reproductive stages. The total plasma proteins also increased during this period to compensate for the high nutritional requirements of the fetus, and these levels remained high until the end of the puerperal phase, when the lambs were weaned. The pregnancy and puerperium influenced all erythrocyte indices and changed the total leukocyte count.

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A Selective and Potent Oral Inhibitor of the JAK2 Tyrosine Kinase Reverses Polycythemia and Leukocytosis Induced by JAK2 V617F in a Mouse Model.

Blood ◽

10.1182/blood.v110.11.557.557 ◽

2007 ◽

Vol 110 (11) ◽

pp. 557-557 ◽

Cited By ~ 2

Author(s):

Virginia M. Zaleskas ◽

Wayne W. Chan ◽

Peter Evangelista ◽

Katherine Lazarides ◽

Rajesh Chopra ◽

...

Keyword(s):

Tyrosine Kinase ◽

Myeloid Leukemia ◽

Leukocyte Count ◽

Peripheral Blood Leukocyte ◽

Jak2 V617f ◽

Myeloproliferative Disease ◽

Short Term ◽

Jak2 Inhibitor ◽

Leukocyte Counts ◽

Blood Leukocyte

Abstract A somatic mutation (V617F) in the pseudokinase domain of the non-receptor tyrosine kinase JAK2 is found in virtually all patients with the myeloproliferative disease (MPD) polycythemia vera (PV), and about half of those with essential thrombocythemia and chronic idiopathic myelofibrosis. When expressed in mice by retroviral bone marrow transduction and transplantation, JAK2 V617F but not JAK2 wild-type induces leukocytosis with neutrophilia and recapitulates the entire erythroid phenotype of PV, with polycythemia, reticulocytosis, low plasma Epo, and endogenous erythroid colonies (Zaleskas et al., PLoS ONE2006;1:e18; Wernig et al., Blood2006;107:4274; Lacout et al., Blood2006;108:1652). By analogy to BCR-ABL in chronic myeloid leukemia, JAK2 V617F is therefore a rational target for therapy in PV and other JAK2 V617F+ MPDs. Previous studies with a nonselective and parenteral tyrphostin JAK2 inhibitor suggested that short-term inhibition of JAK2 could decrease reticulocytosis in recipients of JAK2 V617F-transduced marrow (Zaleskas et al., op cit.). Here, we studied one of a novel series of inhibitors of JAK2, AZ-01, which potently inhibited JAK2 kinase activity (IC50 < 1nM) and suppressed proliferation of cell lines carrying the JAK2 V617F mutation. We treated cohorts of Balb/c mice with JAK2 V617F-induced polycythemia and leukocytosis with AZ-01 at 10 mg/kg by oral gavage twice daily, or with vehicle alone. After 28 days of treatment, AZ-01-treated mice had significant reductions in hematocrit, hemoglobin, reticulocytes, and splenomegaly, as well as normalization of peripheral blood leukocyte counts (Figure 1). These results demonstrate that oral administration of a potent and selective JAK2 inhibitor can reverse the hematopoietic abnormalities of JAK2 V617F-induced MPD in mice, and validate JAK2 as a target for therapy of human JAK2 V617F+ MPD. Figure Figure An orally available JAK2 inhibitor decreases the leukocyte count, hematocrit, and reticulocyte count in mice with JAK2 V617F-induced MPD. Leukocyte count (left), hematocrit (center) and reticulocyte counts (right) of cohorts of mice with JAK2 V617F-induced MPD treated with vehicle or drug at 10 mg/kg BID. Blood counts were assessed at 15 and 28 days of treatment. Decreases in d28 hematocrit and retic count in the drug-treated cohorts were significant (p=0.008 and p<0.0001, respectively, t-tests).

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Leukogram Profile and Clinical Status invivaxandfalciparumMalaria Patients from Colombia

Journal of Tropical Medicine ◽

10.1155/2015/796182 ◽

2015 ◽

Vol 2015 ◽

pp. 1-11 ◽

Cited By ~ 7

Author(s):

Alberto Tobón-Castaño ◽

Esteban Mesa-Echeverry ◽

Andrés Felipe Miranda-Arboleda

Keyword(s):

Hematological Parameters ◽

Clinical Status ◽

Clinical Complications ◽

Cell Counts ◽

Leukocyte Counts ◽

White Blood Cell Counts ◽

Hematologic Profile ◽

Monitoring Treatment ◽

The Relationship ◽

Hematological Alterations

Introduction. Hematological alterations are frequent in malaria patients; the relationship between alterations in white blood cell counts and clinical status in malaria is not well understood. In Colombia, with low endemicity and unstable transmission for malaria, with malariavivaxpredominance, the hematologic profile in malaria patients is not well characterized. The aim of this study was to characterize the leukogram in malaria patients and to analyze its alterations in relation to the clinical status.Methods. 888 leukogram profiles of malaria patients from different Colombian regions were studied: 556 withP. falciparuminfection (62.6%), 313 withP. vivaxinfection (35.2%), and 19 with mixed infection by these species (2.1%).Results. Leukocyte counts at diagnosis were within normal range in 79% of patients and 18% had leucopenia; the most frequent alteration was lymphopenia (54%) followed by monocytosis (11%); the differential granulocyte count in 298 patients revealed eosinophilia (15%) and high basophil counts (8%). Leukocytosis, eosinopenia, and neutrophilia were associated with clinical complications. The utility of changes in leukocyte counts as markers of severity should be explored in depth. A better understanding of these hematological parameters will allow their use in prompt diagnosis of malaria complications and monitoring treatment response.

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Shift Work and Leukocyte Count Changes among Workers in Bangkok

Annals of Work Exposures and Health ◽

10.1093/annweh/wxz039 ◽

2019 ◽

Vol 63 (6) ◽

pp. 689-700

Author(s):

Nitt Hanprathet ◽

Somrat Lertmaharit ◽

Vitool Lohsoonthorn ◽

Thanapoom Rattananupong ◽

Palanee Ammaranond ◽

...

Keyword(s):

Shift Work ◽

Immune Cell ◽

Leukocyte Count ◽

Total Leukocyte Count ◽

Percentage Increase ◽

Cross Sectional ◽

Work Exposure ◽

Leukocyte Counts ◽

Current Shift

Abstract Objectives Previous epidemiological evidence for the association of shift work exposure and increased leukocyte count is cross-sectional in nature, thus limiting cause-effect inference. We therefore used a longitudinal design to: (i) compare leukocyte counts at baseline between shift and day workers and (ii) examine the time trend of leukocyte counts over the follow-up period for these workers. Methods A retrospective cohort study was conducted among 6737 workers aged <60 years at two large organizations (a humanitarian organization and a university) in Bangkok, Thailand who had participated in at least two annual health check-ups during the period 2005–2016. Shift work exposure history was assessed by a self-administered questionnaire and categorized into day, former, and current shift workers. Data on leukocyte count were collected annually as part of worksite health screening during the observation period. Association of shift work exposure and increased leukocyte count was then examined cross-sectionally and longitudinally by using multiple linear regression and multilevel analysis of repeated measures data, respectively. In addition, trends for leukocyte count over the follow-up period and work years were examined using LOWESS smooth curves. Results Compared to day work, the current shift work was associated with increased leukocyte counts. The magnitude of percentage increase was the highest for basophil counts, followed by eosinophil and lymphocyte counts. Both cross-sectional and longitudinal evidence revealed this association, although it was less pronounced longitudinally. For total leukocyte count, the magnitude of difference was constant across the 11-year follow-up period. However, for lymphocyte and basophil counts, these discrepancies tapered over the work years until they no longer differed (for lymphocyte count) or even differed in the opposite direction (for basophil count) in later work years. Conclusion This study confirmed previous cross-sectional evidence that shift work exposure-increased leukocyte counts and that this was reversible. Whether this increase in immune cell count also results in an increased immune cell activity and serves as the intermediary in the association between shift work exposure and subsequent chronic disease development needs further investigation.

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Leukocyte and Monocyte Counts as Predictors for Metabolic Syndrome, Hypertension and Type 2 Diabetes in Taiwan Citizens: a 9-year Longitudinal Study

10.21203/rs.3.rs-141932/v1 ◽

2021 ◽

Author(s):

Yen-Wei Li ◽

Tung-Wei Kao ◽

Chao-Yin Kuo ◽

Pi-Kai Chang ◽

Wei-Liang Chen ◽

...

Keyword(s):

Type 2 Diabetes ◽

Metabolic Syndrome ◽

Longitudinal Study ◽

Multivariate Regression ◽

Health Management ◽

Leukocyte Count ◽

Age Distribution ◽

Total Leukocyte Count ◽

Monocyte Count

Abstract Cardiovascular disease whose correlation with metabolic syndrome and diabetes has been well established. As an aspect of preventive medicine, we conducted a 9-year longitudinal study to identify the relationship between easily measured hematologic parameters, future metabolic syndrome (MetS), hypertension and type 2 diabetes mellitus (T2DM) according to age distribution. Divided into three groups according to age (young age: <40, middle age: ≧40 and <65 and old age: ≧65), 10,328 participants, receiving general medical inspection at the health management centre of Tri-Service General Hospital (TSGH) in Taiwan from 2007 to 2015, had been enrolled in the present study. Through multivariate regression analyses, the hazard ratio (HR) of total and subtype leukocyte count for MetS along with its components, hypertension and T2DM were demonstrated. All MetS components revealed significant association with total leukocyte count in multivariate regression models. Total leukocyte count was associated with future MetS, hypertension and T2DM despite of age at first, but failed in adjusted HR for the old group. Additionally, monocyte count showed significant association with all the aforementioned diseases only in middle-aged subjects but failed in the other two groups. Our results explore the promising value of total leukocyte count and monocyte count to identify the high-risk subjects, especially meddle-aged ones, having MetS, hypertension, and T2DM whether in the present or future.

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Drug-Abuse and Control Populations Differentiated by a Laboratory Profile

Clinical Chemistry ◽

10.1093/clinchem/20.2.172 ◽

1974 ◽

Vol 20 (2) ◽

pp. 172-176

Author(s):

Lewis W Mayron ◽

Ervin Kaplan ◽

Stearly Alling ◽

Jack Becktel

Keyword(s):

Drug Abuse ◽

Polymorphonuclear Leukocyte ◽

Aspartate Aminotransferase ◽

Leukocyte Count ◽

Total Leukocyte Count ◽

Control Group ◽

Drug Abusers ◽

Monocyte Count ◽

Individual Variables ◽

The Individual

Abstract By multivariate analysis of nine laboratory variables, a discriminant function value has been derived that differentiated between a group of 106 drug abusers and a group of 100 normal controls. The variables were serum phosphorus, glucose, lactate dehydrogenase, aspartate aminotransferase, and hematocrit, total leukocyte count, polymorphonuclear leukocyte count, lymphocyte count, and monocyte count. The data demonstrate that the two populations can be distinguished with 4% error in the control group and 14% error in the drug-abuse group. In addition, the individual variables for each group were compared by the t-test. The following were significantly different: serum calcium, phosphorus, blood urea nitrogen, cholesterol, albumin, total bilirubin, alkaline phosphatase, aspartate aminotransferase, and hemoglobin, hematocrit, leukocyte count, polymorphonuclear leukocyte count, stab cell count, and eosinophil count.

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The effect of fermented goat milk (kefir) fortified with vitamin D3 on total leukocyte levels in diabetic Rattus norvegicus rats

Nutrition & Food Science ◽

10.1108/nfs-10-2018-0290 ◽

2019 ◽

Vol 50 (2) ◽

pp. 324-332 ◽

Cited By ~ 1

Author(s):

Reny Marlina ◽

Binar Panunggal ◽

Gemala Anjani

Keyword(s):

Blood Glucose ◽

Vitamin D3 ◽

Rattus Norvegicus ◽

Leukocyte Count ◽

Goat Milk ◽

Total Leukocyte Count ◽

Content Type ◽

Glucose Levels ◽

Blood Glucose Levels ◽

Leukocyte Counts

Purpose Hyperglycemia in type 2 diabetes mellitus increases inflammation through pancreatic beta-cell destruction. Goat milk (kefir) and vitamin D3 have antioxidant and anti-inflammatory properties that can reduce pancreatic beta cells. Increase in total leukocyte count is one of the inflammatory and complication markers of diabetes. This study aims to analyze the effect of fermented goat milk (kefir) fortified with vitamin D3 on total leukocyte counts in diabetic brown rat (Rattus norvegicus). Design/methodology/approach An experimental design with pre- and post-test methodology of control and experimental groups was used. A total of 24 male Wistar rats were divided into four groups: negative control (K−), positive control (K+), kefir treatment (P1) and kefir fortified with vitamin D3 treatment (P2). The control group consisted of healthy rats; the other groups consisted of rats which were given streptozotocin–nicotinamide injections. Before and after intervention, fasting blood glucose levels and total leukocytes counts were measured. Blood glucose levels were analyzed using the GOD–PAP method and leukocyte counts were analyzed using an automatic hematological analyzer. Findings There was a significant decrease in total leukocyte counts in the P2 group (from 26.5 ± 7.7 to 20.4 ± 10.4 [p < 0.05]). However, the decrease in leukocyte count in theP1 group was not significant (from 22.1 ± 9.3 to 16.5 ± 6.2). The decrease in blood glucose levels in theP2 group was −81.4 ± 203.0 but not significant. Originality/value Kefir with vitamin D3 fortification can significantly reduce total leukocyte counts in diabetes.

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Risk of Venous Thrombosis Associated with White Cell Count On Peripheral Blood and Its Interrelationship with Other Environmental Risk Factors

Blood ◽

10.1182/blood.v120.21.1149.1149 ◽

2012 ◽

Vol 120 (21) ◽

pp. 1149-1149

Author(s):

Suely M Rezende ◽

Willem M. Lijfering ◽

Frits R. Rosendaal ◽

Suzanne C. Cannegieter

Keyword(s):

Risk Factors ◽

High Risk ◽

Environmental Risk ◽

Leukocyte Count ◽

Risk Groups ◽

Environmental Risk Factors ◽

White Cell ◽

Patients With Cancer ◽

Leukocyte Counts ◽

Blood Leukocyte

Abstract Abstract 1149 Background: Antithrombotic prophylaxis needs to be balanced against bleeding risk. Therefore, risk stratification is useful to identify patients who would benefit most. In this study, we analyzed how VT risk relates to different leukocyte count. We also analyzed the effect of high leukocyte counts in groups with high risk of VT. Methods: In the MEGA case-control study, blood leukocyte count and information on environmental risk factors were collected from 2443 patients with VT and 1459 partner controls. Logistic regression analyses were adjusted for age and sex. Results: Population characteristics are detailed in Table 1. Risk of VT increased for measurements above the 97.5th percentile for total white cells, granulocytes, lymphocytes and monocytes. Adjusted odds ratio, [OR] for 99th percentile was 1.37 (95% confidence interval [CI], 0.73–2.56), 1.48 (95% CI, 0.79–2.77), 1.20 (95% CI, 0.63–2.31) and 1.88 (95% CI, 1.02–3.46), for total white cells, granulocytes lymphocytes and monocytes, respectively, compared to the reference percentile (5th-95th) (Table 2). We also analysed the effect of high leukocyte counts on VT in high risk groups, including surgical patients, hospitalized patients, and patients with cancer. Adjusted OR for high leukocyte counts (white cell, lymphocyte, monocyte or granulocyte counts > 97.5th percentile) was 6.3 (95% CI, 0.84–47.1) for groups who had surgery or were hospitalized and 2.2 (95% CI, 0.6–8.2) for patients with cancer, respectively, compared with cell counts within the percentile between 5th and 97.5th. Conclusions: High blood leukocyte count is associated with increased risk of VT. The risk is further increased in high risk groups. These results may assist in individually tailored thromboprophylaxis in high risk groups. Disclosures: No relevant conflicts of interest to declare.

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