2017 ◽  
Vol 12 (1) ◽  
pp. 62-75 ◽  
Author(s):  
Renata Bączek-Kwinta

AbstractSwailing as a part of agricultural practice is an illegal habit in many European countries. The indirect effect of swailing is the emission of volatiles (SGV), hence the aim of the study was to identify their impact to seeds of different species occurring or grown Europe. It was carried out on seeds of 29 species of 10 botanical families within the angiosperms. The response to SGV was more or less differentiated within a family, and even within the species, e.g. in the case of tomato. The stimulation of germination and/or increased seedling vigour was established in celery, green- and red-leafed basil, white and red cabbage, white clover and wild thyme. The same effect was noticed for the seeds of stratified broadleaf plantain and the positively photoblastic seeds of German chamomile germinated in darkness. The inhibition of seed germination and/ or reduced seedling vigour was demonstrated in case of caraway, dill and forget-me-not. Similar results were obtained in the experiments carried outin vitroand in the soil, hence it can be assumed that the indirect impact of SGV on plant habitat composition is likely. The interaction of SGV compounds with seed testa and seed phytohormones is discussed.


Author(s):  
Aleksandra A. Jovanović ◽  
Steva M. Lević ◽  
Vladimir B. Pavlovic ◽  
Smilja B. Markovic ◽  
Rada V. Pjanovic ◽  
...  

Freeze drying was compared with spray drying regarding feasibility to process wild thyme drug in order to obtain dry formulations at laboratory scale starting from liquid extracts produced by different extraction methods: maceration, heat-, ultrasound-, and microwave-assisted extractions. Higher powder yield (based on the dry weight prior to extraction) was achieved by freeze than spray drying and lower loss of total polyphenol content (TPC) and total flavonoid content (TFC) due to the drying process. Gelatin as a coating agent (5% w/w) provided better TPC recovery by 70% in case of lyophilization and higher powder yield in case of spray drying by diminishing material deposition on the wall of the drying chamber. The resulting gelatin-free and gelatin-containing powders carried polyphenols in amount ~190 and 53-75 mg gallic acid equivalents GAE/g of powder, respectively. Microwave-assisted extract formulation distinguished from others by higher content of polyphenols, proteins and sugars, higher bulk density and lower solubility. The type of the drying process affected mainly position of the gelatin-derived -OH and amide bands in FTIR spectra. Spray dried formulations compared to freeze dried expressed higher thermal stability as confirmed by differential scanning calorimetry analysis and higher diffusion coefficient; the last feature can be associated with the lower specific surface area of irregularly shaped freeze-dried particles (151-223 µm) compared to small microspheres (~8 µm) in spray-dried powder.


2014 ◽  
Vol 60 (2) ◽  
pp. 7-17 ◽  
Author(s):  
Aneta Wesołowska ◽  
Monika Grzeszczuk ◽  
Dorota Jadczak

Abstract The aim of the studies conducted in 2012-2013 was to compare the chemical composition of essential oils isolated from wild thyme (Thymus serpyllum L.) by hydrodistillation in Deryng and Clevenger apparatus. GC-MS analysis of the isolated oils revealed that carvacrol (42.81-45.24%), γ-terpinene (7.68-9.04%), β-caryophyllene (5.28-9.10%), β-bisabolene (5.76-6.91%) and carvacrol methyl ether (4.92-6.09%) were the major components of all the samples. On the basis of the obtained data it was proved that the type of distillation apparatus had no significant effect on the content of the main essential oil constituents of wild thyme. However, based on the means for both years of the study it was proved that hydrodistillation in Deryng apparatus was more effective for carvacrol concentration, while in Clevenger apparatus - for y-terpinene and carvacrol methyl ether concentration. The type of distillation apparatus had no significant effect on the content of the other essential oil constituents.


2014 ◽  
Vol 32 (No. 5) ◽  
pp. 477-484 ◽  
Author(s):  
R. Malbaša ◽  
J. Vitas ◽  
E. Lončar ◽  
J. Grahovac ◽  
S. Milanović

The antioxidant activity of fermented milk products obtained by using kombucha starter produced by fermentation on sweetened wild thyme extract was investigated. The starter was added to milk containing 0.8, 1.6, and 2.8% milk fat, at fermentation temperature of 37, 40, and 43°C. The fermentation process was terminated when the pH reached 4.5. Antioxidant activities to DPPH and hydroxyl radicals, the contents of MUFAs, PUFAs, vitamin C, and sensory mark, were monitored using the response surface methodology (RSM) and the method of desired function. Kombucha fermented milk products containing wild thyme (WT) showed opposite antioxidant response to DPPH and hydroxyl radicals in terms of milk fat. Optimum processing conditions for WT products in terms of antioxidant activity were: milk fat 2.78% and process temperature 37°C. In order to obtain WT products with a high sensory mark, these conditions are completely different (milk fat 1.10% and process temperature 43°C).  


2020 ◽  
Vol 38 (15_suppl) ◽  
pp. e21657-e21657 ◽  
Author(s):  
Israa Adnan ◽  
Wamidh Talib

e21657 Background: The herbal drinks are highly prevalent in the Middle East and are consumed in large quantities as daily drinks. Few studies have evaluated the biological activities of the herbal drinks produced from some plants.The current study aims to evaluate the anticancer and immunomodulatory activities of five herbal drinks consumed in the Middle East. These drinks are Palestinian Zhourat, Lebanese Zhourat, Lemon and Ginger combination, Wild Thyme, and Marjoram. Methods: The selected herbal drinks as concentrated water extracts were tested separately for: A. Anti-cancer effect on the cell lines (MCF-7, MDA-MB231, HCT-116, A549, and Vero-normal cells) by assessing: - Antiproliferation activities using MTT assay. Apoptosis (using the caspase-3 assay) and vascular endothelial growth factor (VEGF) expression (using ELISA kit) for the most potent antiproliferative extract. B. Immunomodulatory effect by evaluating: - Splenocytes proliferation using the mitogen proliferation assay. Phagocytosis activity using the nitro blue tetrazolium assay. - Pinocytosis function using the neutral red method. Results: The Lemon and Ginger combination was the most potent against MDA-MB231, MCF-7 and A549l cell lines with IC50 3.5,4, 6.5mg/ml, respectively. Lemon and Ginger combination and Wild thyme separately showed high apoptosis induction and angiogenesis suppression on the MDA-MB231cell line at concentrations 3.5 and 4.4 mg/ml for both extracts, respectively. Wild thyme was the most potent stimulant lymphocyte proliferation (with index 4.7), and Marjoram has the highest percentage (314.4%) in phagocytosis activity, while moderate stimulation by Zhourat was noted. Lemon and Ginger combination, Wild thyme, Marjoram, and the Lebanese herbal drink "Zhourat" were the most active extracts in stimulating pinocytosis with absorbance value 0.5745,0.4645,0.461,0.4575 nm, respectively. Meanwhile, the Palestinian herbal drink "Zhourat" had a moderate effect. Conclusions: The consumption ofthe mentioned herbal drinks has various Anti-cancer and immunomodulatory effects. Lemon and ginger combination exhibits the most potent anticancer activities. Wild thyme and marjoram are potent stimulators of innate and acquired immunity. The result achieved in this study is very optimistic and encouraging to be considered for further clinical trials.


2017 ◽  
Vol 67 (4) ◽  
pp. 309-315 ◽  
Author(s):  
Michał Janiak ◽  
Adriana Slavova-Kazakova ◽  
Vessela Kancheva ◽  
Milena Ivanova ◽  
Tsvetelin Tsrunchev ◽  
...  

2016 ◽  
Vol 17 (4) ◽  
pp. 197-202 ◽  
Author(s):  
Milena Rusin ◽  
Janina Gospodarek ◽  
Barbara Biniaś

Molecules ◽  
2020 ◽  
Vol 25 (18) ◽  
pp. 4131
Author(s):  
Marinela Nutrizio ◽  
Gianpiero Pataro ◽  
Daniele Carullo ◽  
Serena Carpentieri ◽  
Luisa Mazza ◽  
...  

The objective of this study was to evaluate the potential of green solvents for extractions of bioactive compounds (BACs) and essential oils from wild thyme (Thymus serpyllum L.) using theoretical and experimental procedures. Theoretical prediction was assessed by Hansen solubility parameters (HSPs) and conductor-like screening model for realistic solvents (COSMO-RS), to predict the most suitable solvents for extraction of BACs. An experimental procedure was performed by nonthermal technology high voltage electrical discharge (HVED) and it was compared with modified conventional extraction (CE). Obtained extracts were analyzed for chemical and physical changes during the treatment. Theoretical results for solution of BACs in ethanol and water, as green solvents, were confirmed by experimental results, while more accurate data was given by COSMO-RS assessment than HSPs. Results confirmed high potential of HVED for extraction of BACs and volatile compounds from wild thyme, in average, 2.03 times higher yield of extraction in terms of total phenolic content was found compared to CE. The main phenolic compound found in wild thyme extracts was rosmarinic acid, while the predominant volatile compound was carvacrol. Obtained extracts are considered safe and high-quality source reach in BACs that could be further used in functional food production.


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