Development of an enzyme-immobilized support using a polyester woven fabric

2016 ◽  
Vol 87 (1) ◽  
pp. 3-14 ◽  
Author(s):  
Eui Jin Shim ◽  
So Hee Lee ◽  
Wha Soon Song ◽  
Hye Rim Kim
Keyword(s):  
Enzyme Immobilization ◽  
Development Process ◽  
Storage Stability ◽  
Treatment Time ◽  
Relative Activity ◽  
Woven Fabrics ◽  
Woven Fabric ◽  
Albumin Concentration ◽  
Bovine Serum Albumin Concentration ◽  
Crosslinking Process

In this study, we aimed to develop an enzyme-immobilized support using polyester woven fabrics and to optimize the development process. We obtained information about the storage stability and reusability of the enzyme and showed the applicability of the polyester woven fabric as an enzyme-immobilized support. In particular, the samples hydrolyzed by hydrogen chloride were treated with N,N’-dicyclohexylcarbodiimide and N-hydroxysuccinimide to activate the surfaces. We evaluated the relative activity of the enzyme immobilization processes, the introduction of spacers, crosslinking and enzyme immobilization and optimized these parameters. The introduction process was controlled to a bovine serum albumin concentration of 1.5% (w/v) and treatment time of 3 h. The crosslinking process was optimized to pH 10.0, a glutaraldehyde concentration of 3% (v/v) and a crosslinking time of 90 min. The immobilization conditions were maintained at pH 8.5, a temperature of 25℃, a time of 45 min and a trypsin concentration of 6% (o.w.f.).

Effect of enzymatic hydrolysis on developing support of polyamide woven fabric for enzyme immobilization

2018 ◽  
Vol 89 (7) ◽  
pp. 1345-1360 ◽  
Author(s):  
Ji Eun Song ◽  
Hye Rim Kim ◽  
So Hee Lee
Keyword(s):  
Enzymatic Hydrolysis ◽  
Enzyme Immobilization ◽  
Structural Changes ◽  
Low Cost ◽  
Relative Activity ◽  
Woven Fabric ◽  
Immobilized Laccase ◽  
Treatment Conditions ◽  
Polyamide Fabric ◽  
Enzyme Molecules

Polyamide fiber has been considered as a suitable support for enzyme immobilization because of its low cost, chemical and mechanical properties and ready availability in a number of different forms. In particular, polyamide fabric has highly specific surface and good elasticity. The present study aims to develop an immobilization support from polyamide fabric and to establish the optimum immobilization conditions for laccase. For this, the enzymatic hydrolysis process was introduced to the hydrolysis of polyamide, creating amino groups that immobilize enzyme molecules. When polyamide fabric was hydrolyzed by bromelain during enzymatic hydrolysis, the highest immobilization yield (68 ± 0.7%) and relative activity (95 ± 0.52%) of immobilized laccase were obtained. For successful enzyme immobilization, the optimal glutaraldehyde crosslinking conditions were pH of 9.0 with 10% (v/v) of glutaraldehyde concentration for 240 min at 45℃. The most favorable immobilization conditions were as follows: pH of 6.0 with 35% (owf) of laccase concentration for 600 min at 4℃. Under the optimum treatment conditions, the pH and thermal stability of immobilized laccase were improved. After 20 days of storage, the immobilized laccase on enzymatic hydrolyzed polyamide fabric retained approximately 30% of its initial activity. Furthermore, the immobilized laccase indicated potential reuse over 10 use cycles. The structural changes of polyamide fabric according to treatment processes were demonstrated by Fourier transform infrared spectroscopy. The changes of surface morphology were measured by scanning electron microscopy according to the multi treatment steps.

scholarly journals α-Cellulose Fibers of Paper-Waste Origin Surface-Modified with Fe3O4 and Thiolated-Chitosan for Efficacious Immobilization of Laccase

Polymers ◽  
2021 ◽  
Vol 13 (4) ◽  
pp. 581
Author(s):  
Gajanan S. Ghodake ◽  
Surendra K. Shinde ◽  
Ganesh D. Saratale ◽  
Rijuta G. Saratale ◽  
Min Kim ◽  
...  
Keyword(s):  
Enzyme Immobilization ◽  
Photoelectron Spectroscopy ◽  
Cellulose Fibers ◽  
Relative Activity ◽  
Significant Activity ◽  
X Ray Diffraction ◽  
X Ray ◽  
Transmission Electron ◽  
Laccase Immobilization ◽  
Surface Modified

The utilization of waste-paper-biomass for extraction of important α-cellulose biopolymer, and modification of extracted α-cellulose for application in enzyme immobilization can be extremely vital for green circular bio-economy. Thus, in this study, α-cellulose fibers were super-magnetized (Fe3O4), grafted with chitosan (CTNs), and thiol (-SH) modified for laccase immobilization. The developed material was characterized by high-resolution transmission electron microscopy (HR-TEM), HR-TEM energy dispersive X-ray spectroscopy (HR-TEM-EDS), X-ray diffraction (XRD), vibrating sample magnetometer (VSM), X-ray photoelectron spectroscopy (XPS), and Fourier transform infrared spectroscopy (FT-IR) analyses. Laccase immobilized on α-Cellulose-Fe3O4-CTNs (α-Cellulose-Fe3O4-CTNs-Laccase) gave significant activity recovery (99.16%) and laccase loading potential (169.36 mg/g). The α-Cellulose-Fe3O4-CTNs-Laccase displayed excellent stabilities for temperature, pH, and storage time. The α-Cellulose-Fe3O4-CTNs-Laccase applied in repeated cycles shown remarkable consistency of activity retention for 10 cycles. After the 10th cycle, α-Cellulose-Fe3O4-CTNs possessed 80.65% relative activity. Furthermore, α-Cellulose-Fe3O4-CTNs-Laccase shown excellent degradation of pharmaceutical contaminant sulfamethoxazole (SMX). The SMX degradation by α-Cellulose-Fe3O4-CTNs-Laccase was found optimum at incubation time (20 h), pH (3), temperatures (30 °C), and shaking conditions (200 rpm). Finally, α-Cellulose-Fe3O4-CTNs-Laccase gave repeated degradation of SMX. Thus, this study presents a novel, waste-derived, highly capable, and super-magnetic nanocomposite for enzyme immobilization applications.

scholarly journals Use of Extended Cover Factor Theory in UV Protection of Woven Fabric

Polymers ◽  
2021 ◽  
Vol 13 (8) ◽  
pp. 1188
Author(s):  
Klara Kostajnšek ◽  
Krste Dimitrovski
Keyword(s):  
Woven Fabrics ◽  
Uv Protection ◽  
Woven Fabric ◽  
Simplified Model ◽  
Light Penetration ◽  
Protective Properties ◽  
Cover Factor ◽  
Coefficient Of Reflection ◽  
Uv Protective Properties

The paper presents an extension of existed cover factor theory more suitable for the evaluation of light penetration through a net woven fabrics structure. It also introduces a new simplified model of predicting the ultraviolet (UV) protective properties of woven fabrics assuming that the coefficient of reflection (KR), transmission (KT), and absorption (KA) of constitutive yarns are known. Since usually they are not, the procedure of preparation of simulation of proper woven fabric samples without interlacing and with known constructional parameters is also presented. The procedure finishes with a fast and cheap detection of missed coefficient for any type of yarns. There are differences between theoretical and measured results, which are not particularly significant in regard to the purpose and demands of investigation.

Immobilization of urease in poly(1-vinyl imidazole)/poly(acrylic acid) network

2010 ◽  
Vol 64 (1) ◽  
pp. 1-7 ◽  
Author(s):  
Mehmet Şenel ◽  
Agah Coşkun ◽  
M. Fatih Abasıyanık ◽  
Ayhan Bozkurt
Keyword(s):  
Acrylic Acid ◽  
Enzyme Immobilization ◽  
Storage Stability ◽  
Polymer Network ◽  
Ft Ir ◽  
Basic Characteristics ◽  
And Storage ◽  
Menten Constant ◽  
Ft Ir Spectroscopy ◽  
Poly Acrylic Acid

AbstractIn this study, urease was immobilized in a polymer network obtained by complexation of poly(1-vinyl imidazole) (PVI) with poly(acrylic acid) (PAA). Preparation of the polymer network was monitored by FT-IR spectroscopy. Scanning electron microscopy (SEM) revealed that enzyme immobilization had a strong effect on film morphology. Proton conductivity of the PVI/PAA network was measured via impedance spectroscopy under humidified conditions. Values of the Michaelis-Menten constant (K M) for immobilized urease were higher than for the free enzyme, indicating a decreased affinity of the enzyme to its substrate. The basic characteristics (pHopt, pHstability, T opt, T stability, reusability, and storage stability) of immobilized urease were determined. The results show that the PAA/PVI polymer network is suitable for enzyme immobilization.

Prediction of Permeability Tensor for Plain Woven Fabric by Using Control Volume Finite Element Method

2003 ◽  
Vol 11 (6) ◽  
pp. 465-476 ◽  
Author(s):  
Y. S. Song ◽  
K. Chung ◽  
T. J. Kang ◽  
J. R. Youn
Keyword(s):  
Finite Element Method ◽  
Finite Element ◽  
Control Volume ◽  
Three Dimensional ◽  
Woven Fabrics ◽  
Permeability Tensor ◽  
Woven Fabric ◽  
Stacking Order ◽  
Control Volume Finite Element ◽  
Element Method

The complete prediction of the second order permeability tensor for a three dimensional multi-axial preform is critical if we are to model and design the manufacturing process for composites by considering resin flow through a multi-axial fiber structure. In this study, the in-plane and transverse permeabilities for a woven fabric were predicted numerically by the coupled flow model, which combines microscopic and macroscopic flows. The microscopic and macroscopic flows were calculated by using 3-D CVFEM(control volume finite element method) for micro and macro unit cells. To avoid a checkerboard pressure field and improve the efficiency of numerical computation, a new interpolation function for velocity is proposed on the basis of analytical solutions. The permeability of a plain woven fabric was measured by means of an unidirectional flow experiment and compared with the permeability calculated numerically. Reverse and simple stacking of plain woven fabrics were taken into account and the relationship between the permeability and the structures of the preform such as the fiber volume fraction and stacking order is identified. Unlike other studies, the current study was based on a more realistic three dimensional unit cell. It was observed that in-plane flow is more dominant than transverse flow within the woven perform, and the effect of the stacking order of a multi-layered preform was negligible.

Manufacturing Process and Property Evaluation of Functional Composite Yarn-Dyed Woven Fabrics Made from Bamboo Charcoal/Stainless Steel and TPU

2008 ◽  
Vol 55-57 ◽  
pp. 413-416 ◽  
Author(s):  
C.I. Huang ◽  
C.I. Su ◽  
Ching Wen Lou ◽  
Wen Hao Hsing ◽  
Jia Horng Lin
Keyword(s):  
Stainless Steel ◽  
Manufacturing Process ◽  
Far Infrared ◽  
Woven Fabrics ◽  
Woven Fabric ◽  
Weft Yarn ◽  
Bamboo Charcoal ◽  
Functional Composite ◽  
Composite Yarn ◽  
Composite Fabrics

Recently, development of technology increases human life quality and gradually raises the value of health protection in human’s concept. Bamboo has multi-functional including far infrared radiation, deodorization and anion generation. Therefore, bamboo charcoal has been widely used in textile industry. Moreover, development of technology also increased the electromagnetic hazard in human’s daily life. This study aims to develop a manufacturing process of functional composite yarn-dyed woven fabrics. In the manufacturing process, the materials included pure cotton yarn, stainless steel fiber(called metallic yarn) and viscose rayon yarn containing bamboo charcoal (called bamboo charcoal yarn) were used for making the bamboo charcoal/stainless steel composite woven fabric. The composite woven fabrics were woven by using same warp yarn and two kinds of weft yarn that contained bamboo charcoal and stainless steel. The composite fabrics had two different structures. Those fabrics were changed the order of bamboo charcoal yarn and metallic yarn. The ratios of weft yarn were 1 end of bamboo charcoal yarn to 1 end of metallic yarn and 3 ends of bamboo charcoal yarn to 1 end of metallic yarn. Furthermore, the fabrication of composite fabrics that included plain, 2/2 twill and dobby were changed. The composite woven fabrics were finished and laminated by TPU film to enhance the waterproof and vapor permeable functions. The laminated composite fabrics were evaluated by far-infrared coefficient, anion generation rate, water vapor permeability, water resistance, surface electric resistance and electromagnetic shelter property to obtained optimal manufacturing process.

scholarly journals Application of Natural Dyes and Sodium Alginate From Sargassum Sp. Sea weed In Coloring Bima Woven Fabric

2020 ◽  
Vol 36 (05) ◽  
pp. 964-967
Author(s):  
Agrippina Wiraningtyan ◽  
Ruslan Ruslan ◽  
Putri Ayu Mutmainnah ◽  
Magfirah Perkasa
Keyword(s):  
Surface Structure ◽  
Sodium Alginate ◽  
Morphological Analysis ◽  
Woven Fabrics ◽  
Natural Dyes ◽  
Woven Fabric ◽  
Dyeing Process ◽  
Wave Numbers ◽  
Absorption Pattern ◽  
Before And After

This study aims to extract dye and alginate from seaweed Sargassum sp. as a dye paste in the coloring of Bima woven fabric. The concentration of sodium alginate used was 0%; 1%; 3% and 5%. The results showed that the absorbance value of the dye extract from seaweed Sargassum sp at maximum λ = 203 nm obtained A = 3.899. The effect of variations in the concentration of sodium alginate in the dye paste was determined by comparing the FTIR absorption pattern of Bima woven fabrics. Based on the FTIR absorption pattern data, it was found that a mixture of dye and sodium alginate of 3% had a stronger intensity, namely the wave numbers 3448.72 cm-1 and 1635 cm-1; 2900.94 cm-1; 2337.72 cm-1; 1381.03 cm-1 and 1064.71 cm-1. The results of the morphological analysis showed significant differences in surface structure on Bima woven fabrics before and after the dyeing process.

scholarly journals Limitations of the CAD-CAM System in the Process of Weaving

2020 ◽  
Vol 0 (0) ◽  
Author(s):  
Stana Kovačević ◽  
Snježana Brnada ◽  
Irena Šabarić ◽  
Franka Karin
Keyword(s):  
Woven Fabrics ◽  
Virtual Simulation ◽  
Woven Fabric ◽  
Virtual Image ◽  
Computer Screen ◽  
Cad Cam ◽  
Fabric Structures

AbstractThe weaving process is constantly evolving in terms of productivity, quality, and possibilities of fabrication of different fabric structures and shapes. This article covers some issues that have still not been resolved and represents distracting factors in the woven fabrics production. In the development of woven fabric using the CAD technology, it is inevitably a deviation of the virtual image on the computer screen from the woven sample. According to comprehensive industry analyses, the findings of many authors who contributed to the resolution of these problems can be concluded that these problems are still present in the development and production of striped, checkered, and jacquard woven fabrics. In this article, jacquard, multicolor woven fabrics were investigated, with deviations in pattern sizes and shades of color in warp and weft systems compared to virtual simulation on the computer, as well as the tendency of the weft distortion arising from the weaving process leading to the pattern deformation.

scholarly journals Bentonite-supported catalase

2005 ◽  
Vol 70 (5) ◽  
pp. 721-726 ◽  
Author(s):  
S. Alkan ◽  
H. Ceylan ◽  
O. Arslan
Keyword(s):  
Ionic Strength ◽  
Kinetic Parameters ◽  
Enzyme Immobilization ◽  
Storage Stability ◽  
High Ionic Strength ◽  
Enzyme Inactivation ◽  
Valuable Method ◽  
And Storage

The properties of the clay bentonite as a support for enzyme immobilization were studied using the enzyme catalase. Such an immobilization does not result in enzyme inactivation and constitutes a valuable method for immobilizing catalase at high ionic strength. The bentonite-supported catalase was characterized in terms of pH and ionic strength dependencies, thermal and storage stability and kinetic parameters. These studies indicate that bentonite is a valuable support for the simple adsorption of enzymes. .

scholarly journals EFFECT OF SPANDEX DENIER OF WEFT CORE SPUN YARN ON PROPERTIES OF FINISHED STRETCH WOVEN FABRIC

2020 ◽  
Vol 7 (7) ◽  
pp. 21-32
Author(s):  
Sunny Pannu ◽  
Meenakshi Ahirwar ◽  
Rishi Jamdigni ◽  
B. K. Behera
Keyword(s):  
Research Work ◽  
Woven Fabrics ◽  
Woven Fabric ◽  
The Core ◽  
Spun Yarn ◽  
Spun Yarns ◽  
Recovery Percentage ◽  
Shape Retention ◽  
Dual Core ◽  
Made In

The woven fabrics containing cotton/spandex core spun yarns possesses very vital properties of stretch, recovery and thus shape retention from the view point of wearing comfort and garment appearance. Spandex present in the core of core spun yarn is the most essential performer behind these properties. An attempt is made in this research work to study the influence of changing spandex denier in core spun yarn on the stretch and functional properties of stretch woven fabrics. The sole objective of this study is to find out whether different stretch, shrinkage and physical properties of stretch woven fabrics depend upon changing spandex percentage achieved by means of change in spandex filament denier. It was observed that by increasing denier of spandex in core spun weft yarns the increase in weft shrinkage diminishes. Dual core weft with spandex provides good elongation percentage and recovery percentage. The fabric with higher denier spandex in yarn shows a decreasing total hand values trend for summer and winter. The results depicts that the fabrics have higher THV for winter suiting fabrics as compared to summer suiting thus are more suitable for the winter wear.

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